CN103571900A - Preparation and efficacy of low molecular weight soybean peptide - Google Patents
Preparation and efficacy of low molecular weight soybean peptide Download PDFInfo
- Publication number
- CN103571900A CN103571900A CN201210252517.0A CN201210252517A CN103571900A CN 103571900 A CN103571900 A CN 103571900A CN 201210252517 A CN201210252517 A CN 201210252517A CN 103571900 A CN103571900 A CN 103571900A
- Authority
- CN
- China
- Prior art keywords
- soybean
- molecular weight
- soybean peptide
- preparation
- soybean peptides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Peptides Or Proteins (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention belongs to the field of soybean protein deep processing technology, and specifically relates to a method used for extracting soybean peptide via enzyme-membrane method. The method comprises following steps: commercially available soybean isolate protein is taken as a raw material, and is subjected to alkali dissolution, sterilization processing, and enzyme hydrolysis using protease so as to obtain a crude soybean peptide liquid; the crude soybean peptide liquid is processed via novel membrane treatment technology and nanofiltration, and a filtrate containing peptide is obtained via filtration, wherein the molecular weight of most of the peptide is less than 1000D; and the filtrate is subjected to sterilization, and spray drying so as to obtain a soybean peptide powder, wherein more than 80% of the soybean peptide powder is the soybean peptide with the molecular weight less than 1000D, a solution of the soybean peptide powder is clear, and no turbidity is caused by refrigeration of the soybean peptide powder in acid solution. The soybean peptide powder can be used in acid food and various beverages, and possesses excellent flavors and excellent characteristics such as easy absorption.
Description
Technical field
The present invention relates to soybean protein deep process technology field, be specifically related to a kind of enzyme membrane method and extract the method for soybean peptides, be a kind ofly to take commercially available soybean protein isolate as raw material, utilize biological enzyme enzymolysis and through ultrafiltration, nanofiltration separation, prepare molecular weight lower than the soybean peptides of 1000D.
Background technology
Soybean peptides, with respect to soybean protein and amino acid, more easily absorb, and absorption rate is faster.Soybean Toplink improves body metabolism speed, reduces body fat content; Picked-up soybean peptides, has the temperature that increases health, the effect that ameliorate body is cold.Soybean peptides has been proved to be has the stress of alleviating, and improves the effect of attention.For mental worker and student Chang You feel sleepy, the problem such as hypomnesis, human thinking are blunt, soybean peptides health-nutrition product can be eliminated brain fag fast, improve attention, keep resourceful, alleviate stress.Soybean Toplink alleviates body muscle damage, promotes the recovery of physical fatigue.The crowd who often drinks for need of work, soybean Toplink reduces blood alcohol concentration, alleviates after drinking the malaise symptoms such as headache.
The raw material sources of soybean peptides is more extensive, has de-fatted soybean dregs, defatted soy flour, soybean protein isolate etc.; So far, the precursor soybean protein isolate of soybean peptides is prepared by following method conventionally: in skimmed soy beans, add water to make defatted soymilk slurry, with separated " bean dregs " removed wherein of centrifugal method, in the water-soluble portion (defatted soymilk liquid) generating, add acid to adjust its pH value to the iso-electric point of soybean protein, make soybean protein precipitation, separation and removal supernatant liquor (soya-bean milk is clear), thus acid soya-bean milk liquid obtained; Add alkali and neutralize this acid soya-bean milk liquid, by the method such as dry of spraying, be dried and obtain described " soybean protein isolate (protein content is more than 90%).Soybean peptides production technique also generally adopts following steps, and soybean protein isolate is dry through dissolving, enzymolysis, centrifugation, sterilization, flash distillation deodorization, spraying, stdn, packaging final prod.Gained soybean peptides molecular weight product is large, and does not concentrate, and is difficult for fully absorbing, and may form precipitation, the inadequate clear of gained soybean peptides solution in acidic solution.The new membrane treatment technology that we adopt is different from traditional centrifugal separation technique, is characterized in obtained soybean peptides solution clear, is easily absorbed by the body.
Alcalase proteolytic enzyme, claims again Bacillus licheniformis proteolytic enzyme; It is by the extract after microorganism Bacillus licheniformis (Bacillus licheniformis) fermentation, is one of inscribe proteolytic ferment.Zymetology classification (EC): 3.4.21.62.Be used for transesterification (base) or turn peptide (base) reaction and using milk casein as raw material preparation is as bioactive peptide---phosphopeptide protective foods, that include 25～37 amino-acid residues.Unit of enzyme activity definition: under 7.5,37 ℃ of conditions of pH value, per minute discharges 1 μ mol from casein can make amino acid that forint (Folin) reagent is positive and the enzyme Liang Weiyige unit of enzyme activity of peptide (in tyrosine).UHT,
ultra High Temperature treatedthe instantaneous processing of ultrahigh-temperature, Ultra High Temperature Short Time temperature is 135-140 ℃, is incubated for 4 seconds, to reach commercially sterilised requirement, is a kind of sterilization process of fresh milk, cereal beverage, beverage processing, can be without preserving at normal temperatures, the quality guaranteed period can reach 1-6 month.
Summary of the invention
The object of the invention is to provide a kind of method of preparing lower molecular weight soybean peptides by enzymic hydrolysis and new membrane treatment technology.The soybean peptides peptide content obtaining is high, and molecular weight is relatively concentrated, and assimilated efficiency significantly improves.
The preparation of lower molecular weight soybean peptides, the steps include:
(a) take commercially available soybean protein isolate as raw material, at 20 ℃～45 ℃, soybean protein isolate is carried out to alkali molten, add saturated NaOH solution, adjusting pH is 5～9;
(b) above-mentioned soybean protein isolate alkaline solution temperature is remained on to 40 ℃～70 ℃, according to 1 ‰ of soybean protein isolate weight～10 ‰, add Alcalase proteolytic enzyme, stir enzymolysis 1h～8h;
(c) at room temperature thick soybean peptides liquid is carried out to new membrane and process operation, the new membrane aperture of employing is 0.05 μ m～0.20 μ m, and leaching molecular weight is 300～3000D, and pressure is 0.2MPa～1.0MPa, collects and leaches soybean peptides mixed solution; At room temperature soybean peptides mixed solution is carried out to nanofiltration operation, the molecular weight cut-off of the nanofiltration membrane of employing is 150～300D, and pressure is 0.3MPa～0.8MPa, collects trapped fluid;
(d) trapped fluid of collecting after nanofiltration is carried out to spray drying treatment after by UHT sterilizing, obtain pulverous soybean peptides, wherein more than 80% peptide molecular weight is less than 1000D, solution clear.
Adopt method of the present invention, the soybean peptides of preparation, lower molecular weight soybean peptides lower than 1000D accounts for more than 80%, its solution clear, in acidic solution, refrigeration does not form muddiness yet, can be applicable to, in acidic food and various drink, to have good taste, this product has the good characteristics such as easy absorption.
Embodiment
Illustrate below and realize implementation of the present invention: (a) take commercially available soybean protein isolate as raw material, at 20 ℃～45 ℃, soybean protein isolate is carried out to alkali molten, add saturated NaOH solution, adjusting pH is 5～9;
(b) above-mentioned soybean protein isolate alkaline solution temperature is remained on to 40 ℃～70 ℃, according to 1 ‰ of soybean protein isolate weight～10 ‰, add Alcalase proteolytic enzyme, stir enzymolysis 1h～8h;
(c) at room temperature thick soybean peptides liquid is carried out to new membrane and process operation, the new membrane aperture of employing is 0.05 μ m～0.20 μ m, and leaching molecular weight is 300～3000D, and pressure is 0.2MPa～1.0MPa, collects and leaches soybean peptides mixed solution; At room temperature soybean peptides mixed solution is carried out to nanofiltration operation, the molecular weight cut-off of the nanofiltration membrane of employing is 150～300D, and pressure is 0.3MPa～0.8MPa, collects trapped fluid;
(d) trapped fluid of collecting after nanofiltration is carried out to spray drying treatment after by UHT sterilizing, obtain pulverous soybean peptides, wherein more than 80% peptide molecular weight is less than 1000D, solution clear.
The enzyme that above-mentioned steps is used is Sumizyme MP or neutral protease; Comprise endo-type and circumscribed-type; Wherein, (b) temperature range in step, according to the reference range of temperature of enzyme used, carries out the screening of temperature condition; PH value in method, carries out conditional filtering according to the reference pH scope of enzyme used; (c) the molecular weight 300-3000D that the new membrane technology of using in step is held back; Wherein, the molecular-weight average of soybean peptide mixture is lower than 1000D.
Below enumerate embodiment and further explain the present invention, but the present invention is not subject to the restriction of these specific embodiments.
Embodiment 1: take soybean protein isolate as raw material, add alkalescence or neutral protease, under fixed temperature condition, select soybean peptides molecular weight distribution that different PH draw and the clarity of solution, we evaluate the clarity of solution according to the size of OD610nm value.
Embodiment 2: take soybean protein isolate as raw material, add alkalescence or neutral protease, under fixed temperature condition, select soybean peptides molecular weight distribution that differing temps draws and the clarity of solution.
Embodiment 3: take soybean protein isolate as raw material, add alkalescence or neutral protease, 40～70 ℃ of temperature, enzymolysis 1h～8h under the condition of PH5～9, by aperture, be the film of 0.1 μ m～0.45 μ m, collecting the filtrate obtaining is the soybean peptides mixed solution that we need; Peptide molecular weight and filtrate clarity result that the method for traditional separation method and membrane filtration of the present invention of contrasting draws are as follows:
What more than enumerate is only to realize several specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, can also have many distortion, repeat no more herein.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (5)
1. the preparation of lower molecular weight soybean peptides, is characterized in that:
(a) take commercially available soybean protein isolate as raw material, at 20 ℃～45 ℃, soybean protein isolate is carried out to alkali molten, add saturated NaOH solution, adjusting pH is 5～9;
(b) above-mentioned soybean protein isolate alkaline solution temperature is remained on to 40 ℃～70 ℃, according to 1 ‰ of soybean protein isolate weight～10 ‰, add Alcalase proteolytic enzyme, stir enzymolysis 30min～8h;
(c) at room temperature thick soybean peptides liquid is carried out to new membrane and process operation, the new membrane aperture of employing is 0.05 μ m～0.20 μ m, and leaching molecular weight is 300～3000D, and pressure is 0.2MPa～1.0MPa, collects and leaches soybean peptides mixed solution; At room temperature soybean peptides mixed solution is carried out to nanofiltration operation, the molecular weight cut-off of the nanofiltration membrane of employing is 150～300D, and pressure is 0.3MPa～0.8MPa, collects trapped fluid;
(d) trapped fluid of collecting after nanofiltration is carried out to spray drying treatment after by UHT sterilizing, obtain pulverous soybean peptides, wherein more than 80% peptide molecular weight is less than 1000D, solution clear.
2. the preparation of lower molecular weight soybean peptides according to claim 1, is characterized in that: the enzyme using in described method steps is Sumizyme MP or neutral protease; Comprise endo-type and circumscribed-type.
3. the preparation of lower molecular weight soybean peptides according to claim 1, is characterized in that: temperature range in described method steps, according to the reference range of temperature of enzyme used, carry out the screening of temperature condition; According to the reference pH scope of enzyme used, carry out pH conditional filtering.
4. the preparation of lower molecular weight soybean peptides according to claim 1, is characterized in that: the molecular weight that the new membrane technology that described method steps is used is held back is 300～3000D.
5. the preparation of lower molecular weight soybean peptides according to claim 1, is characterized in that: in described method steps, the molecular-weight average of soybean peptide mixture is lower than 1000D.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210252517.0A CN103571900A (en) | 2012-07-20 | 2012-07-20 | Preparation and efficacy of low molecular weight soybean peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210252517.0A CN103571900A (en) | 2012-07-20 | 2012-07-20 | Preparation and efficacy of low molecular weight soybean peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103571900A true CN103571900A (en) | 2014-02-12 |
Family
ID=50044610
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210252517.0A Pending CN103571900A (en) | 2012-07-20 | 2012-07-20 | Preparation and efficacy of low molecular weight soybean peptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103571900A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222493A (en) * | 2014-07-25 | 2014-12-24 | 青岛尚德生物技术有限公司 | Compound probiotic peptide as well as preparation method and application thereof |
| CN105614899A (en) * | 2016-01-13 | 2016-06-01 | 刘新旗 | Low-molecular soybean peptide and preparation method thereof |
| CN105949274A (en) * | 2016-07-08 | 2016-09-21 | 刘新旗 | Novel soybean peptide separating and refining method |
| CN106858236A (en) * | 2017-01-05 | 2017-06-20 | 东北农业大学 | A kind of method that aqueous enzymatic method Soy hydrolysate prepares polypeptide compound beverage |
| CN107674900A (en) * | 2017-06-12 | 2018-02-09 | 诺利如（安阳）生物科技有限公司 | A kind of process using series connection film concentration Soybean Peptide |
| CN108208309A (en) * | 2017-12-28 | 2018-06-29 | 保龄宝生物股份有限公司 | The production method of low ash content soybean peptide containing profitable probliotics |
| CN110623137A (en) * | 2019-10-30 | 2019-12-31 | 创客商盟有限公司 | Soybean peptide product with small molecular weight unit and preparation method thereof |
| CN112640986A (en) * | 2020-08-12 | 2021-04-13 | 邹鹏 | Production process of soybean oligopeptide |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102094059A (en) * | 2009-12-10 | 2011-06-15 | 陈栋梁 | Method for preparing soybean polypeptide by microwave-promoted hydrolysis of soybean protein |
-
2012
- 2012-07-20 CN CN201210252517.0A patent/CN103571900A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102094059A (en) * | 2009-12-10 | 2011-06-15 | 陈栋梁 | Method for preparing soybean polypeptide by microwave-promoted hydrolysis of soybean protein |
Non-Patent Citations (1)
| Title |
|---|
| 卢鹤等: "高效液相色谱法分离大豆蛋白肽", 《齐齐哈尔大学学报》, vol. 22, no. 2, 31 March 2006 (2006-03-31), pages 1 - 3 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104222493A (en) * | 2014-07-25 | 2014-12-24 | 青岛尚德生物技术有限公司 | Compound probiotic peptide as well as preparation method and application thereof |
| CN104222493B (en) * | 2014-07-25 | 2016-08-31 | 青岛尚德生物技术有限公司 | A kind of compound benefit bacterium peptide and its preparation method and application |
| CN105614899A (en) * | 2016-01-13 | 2016-06-01 | 刘新旗 | Low-molecular soybean peptide and preparation method thereof |
| CN105614899B (en) * | 2016-01-13 | 2019-02-01 | 刘新旗 | A kind of low molecule soybean peptide and preparation method thereof |
| CN105949274A (en) * | 2016-07-08 | 2016-09-21 | 刘新旗 | Novel soybean peptide separating and refining method |
| CN106858236A (en) * | 2017-01-05 | 2017-06-20 | 东北农业大学 | A kind of method that aqueous enzymatic method Soy hydrolysate prepares polypeptide compound beverage |
| CN107674900A (en) * | 2017-06-12 | 2018-02-09 | 诺利如（安阳）生物科技有限公司 | A kind of process using series connection film concentration Soybean Peptide |
| CN107674900B (en) * | 2017-06-12 | 2021-05-14 | 诺利如一（安阳）生物科技有限公司 | Process method for concentrating soybean peptide by adopting tandem membrane |
| CN108208309A (en) * | 2017-12-28 | 2018-06-29 | 保龄宝生物股份有限公司 | The production method of low ash content soybean peptide containing profitable probliotics |
| CN110623137A (en) * | 2019-10-30 | 2019-12-31 | 创客商盟有限公司 | Soybean peptide product with small molecular weight unit and preparation method thereof |
| CN112640986A (en) * | 2020-08-12 | 2021-04-13 | 邹鹏 | Production process of soybean oligopeptide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103571900A (en) | Preparation and efficacy of low molecular weight soybean peptide | |
| JP2020073578A (en) | Plant extracts containing diketopiperazines and methods of making | |
| CN102048022B (en) | Soybean polypeptide without hydrolysis and bitter tastes as well as preparation method and application thereof | |
| CN102696767B (en) | A kind of deep working method fully utilizing walnut kernel production low fat Walnut Milk, active peptide, albumen powder | |
| CN100548150C (en) | The preparation method of coconut extractive | |
| CN101133813B (en) | Concentrated cucumber clear juice and method for producing the same | |
| CN104621253A (en) | Activated yellow slurry water lactic acid beverage and production process thereof | |
| CN101455428B (en) | Walnut peptide nutrient food and preparation method thereof | |
| CN103053984B (en) | Kelp seasoning wine and preparation method thereof | |
| CN101766253B (en) | Method for preparing rice protein polypeptide powder from rice residue protein | |
| CN102640796A (en) | Peptide-containing low molecular plant protein milk and production method thereof | |
| CN101288437B (en) | Method for producing soy protein peptides and dietary fiber from defated soybean pulp | |
| CN102994598B (en) | Weak bitter corn oligopeptide with high content of alanine and leucine, and preparation method thereof | |
| CN101731683B (en) | Technique for preparing almond peptide sports beverage and separating almond oil by enzyme process | |
| CN110089574A (en) | A kind of preparation method and its prepared soya-bean milk, underflow and bean powder of the full beans bean powder containing peptide | |
| CN102106581A (en) | Method for processing weak alkali pine needle beverage | |
| AU2020103351A4 (en) | Preparation method of green plum essence | |
| CN102835504B (en) | Oil orange green tea drink | |
| CN103519296A (en) | Processing method of clear cucumber juice with good taste | |
| CN105777926A (en) | Method for comprehensively extracting effective components from walnuts | |
| CN106820113A (en) | Full clover peptide nutrient food and preparation method thereof | |
| CN106472758A (en) | A kind of processing technique of green tea beverage | |
| CN103005597A (en) | Mulberry leaf water drink and method for preparing same | |
| CN101099532A (en) | High purity instant protein powder producing method | |
| CN102273517A (en) | Trichosanthes kirilowii Maxim seed plant protein beverage and production method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140212 |