CN106106146B - Tissue culture system and its planar emission apparatus, LED light light incubator - Google Patents

Tissue culture system and its planar emission apparatus, LED light light incubator Download PDF

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Publication number
CN106106146B
CN106106146B CN201610442519.4A CN201610442519A CN106106146B CN 106106146 B CN106106146 B CN 106106146B CN 201610442519 A CN201610442519 A CN 201610442519A CN 106106146 B CN106106146 B CN 106106146B
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tissue culture
emission apparatus
conveying
light
planar emission
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CN106106146A (en
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朱孔海
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Zhejiang Haixing Pharmaceutical Co ltd
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ZHEJIANG HAIXING BITECHNOLOGY Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The invention discloses tissue culture system and its planar emission apparatus, LED light light incubator, the planar emission apparatus includes light guide plate, LED aluminum base plate and heat dissipation wing plate, LED aluminum base plate front is provided with multiple red LED lamps and multiple blue LED lamps in a manner of array distribution, the incidence surface of light guide plate is close to the back side of aluminum substrate close to the front of aluminum substrate, heat dissipation wing plate;One planar emission apparatus is located at chain conveying circulation inside, another planar emission apparatus is located at chain conveying circulation outside, their vertical plane progress illumination inside and outside to conveying circulation respectively;And both are separately fixed in two mobile bases, two mobile bases are slidably connected with babinet by track level, and drive two planar emission apparatus respectively inside and outside synchronously closer or far from the vertical plane by illumination of conveying circulation by screw.This programme can conveniently adjust light quality ratio, intensity of illumination and the periodicity of illumination of red blue mixed light.

Description

Tissue culture system and its planar emission apparatus, LED light light incubator
The application is
Application No.:201510299391.6
The applying date is:On June 4th, 2015
Apply entitled:Tissue culture system
Patent application divisional application.
Technical field
The present invention relates to a kind of tissue culture system for plants such as dendrobium candidums.
Background technology
Dendrobium candidum, is one of kind the most precious in China's orchid family Dendrobium Sw.Because there is enhancing body to exempt from for it Epidemic disease power, antitumor, anti-aging and the effectively effect such as mitigation hepatic injury, in version in 2010《Pharmacopoeia of People's Republic of China》Middle coverlet Medicinal material simply solely is classified as, due to artificial excessively excavation and the destruction of ecological environment, its own low reproduction rate in addition, wild iron sheet stone Dry measure used in former times resource is increasingly exhausted, is classified as the rare or endangered species of two level focused protection by country.Now, artificial cultivation plantation iron sheet stone Dry measure used in former times becomes the main path for meeting great market demand.Dendrobium candidum system orchid family Dendrobium Sw, seed is minimum, without endosperm, Need to sprout with some mycosymbiosises under natural conditions, germination percentage only a ten thousandth in the case of nature, and cultivar exists Degradation phenomena.
In recent years, some scientific research institutions in China and business unit carry out the tissue culture and artificial cultivation technique of dendrobium candidum Substantial amounts of exploration and experiment, achieve some achievements, also so that artificial cultivation dendrobium candidum yield increased in recent years.But It is that the artificial culture of dendrobium candidum still has that seeding propagation speed is slow, training tissue culture seedling and transplanting survival rate are low, artificial growth The problem of product active constituent content is low, seriously constrains industrialization, the large-scale development of dendrobium candidum.In addition, dendrobium candidum General field planting can be with growth of weeds, and be vulnerable to pest and disease damage.
The patent document of Publication No. CN102057867A discloses a kind of light tune of the tissue cultures of seedlings of Dendrobium officinale Prosecutor method, including powdered seed is obtained after being sterilized by Dendrobium officinale fruit, powdered seed is trained by seed germination medium Support, sprout the process for forming protocorm, it is characterised in that in the tissue culture procedures of seedlings of Dendrobium officinale, (semiconductor is sent out with LED Optical diode) plant growth lighting system as light source, according to the different phase of dendrobium candidum tissue culture, implements different light According to processing.Implement different photo-irradiation treatments by using different stages of growth, seedlings of Dendrobium officinale is maximally utilised light Its photosynthesis can be supplied, so as to obtain maximum growth amount.
In the tissue culture system of the prior art, light quality ratio, the intensity of illumination of red blue mixed light can not be conveniently and efficiently adjusted And periodicity of illumination.
The content of the invention
In order to solve above-mentioned technical problem, the object of the present invention is to provide a kind of tissue culture system, LED light optical culture is inside set Case, the light quality ratio, intensity of illumination and illumination week of red blue mixed light can be conveniently adjusted using planar emission apparatus Phase.
To achieve the above object, present invention employs following technical solution:
A kind of tissue culture system, including totally enclosed tissue culture transfer room and artificial training seedling room, the tissue culture transfer room and people Work training seedling room or so is disposed adjacent, and the entrance of tissue culture transfer room and artificial training seedling room is connected with the outlet of Airshower chamber, described group Multiple vaccination stations and a conveyer belt are set in training transfer room, and the output terminal of conveyer belt is stretched into manually in training seedling room, described artificial Train and training seedling interior room is equipped with seedling room, manually train between seedling room and training seedling interior room only by several ventilation window ventilating airs, from Seedling room is manually trained by a sliding door into training seedling interior room, is trained indoor equipped with constant-temperature constant-humidity air-conditioner and the training of multiple LED lamplights in seedling Support case;Several ventilation fans connected with outside are installed on the wall of the manually training seedling room, manually train the outside peace of seedling room Equipped with steel pipe framework, sunshade net is laid with the top of steel pipe framework, sunshade net is furled and is unfolded by drawstring, is provided with steel pipe framework For the pulley of drawstring traction;The LED light light incubator includes babinet, sets tissue culture bottle conveying device and more in babinet respectively A planar emission apparatus;Tissue culture bottle conveying device includes multiple chains, and each chain is arranged on by sprocket wheel in babinet and head and the tail Connection is formed in the motion cycle in vertical plane, and the motion cycle of multiple chains is parallel to each other and is driven by same driving device same Step is moved, and multiple loop brackets that can place tissue culture bottle is set between adjacent chain, in this way, multiple chains are just formed in vertically The structure for conveying of circulation conveying tissue culture bottle in face, loop bracket include filler ring and the connecting shaft being fixedly connected, group are put into filler ring Bottle is trained, tissue culture bottle is fixed in filler ring by the flange bearing on bottle or bottle cap circumference, the connecting shaft and chain of loop bracket Link units connection, which includes outer plate, inner plate, roller, sleeve and self lubrication bearing, and two inner plates lead to Cross sleeve and be fixedly connected to form inner link, bush outer sleeve sets roller, and self lubrication bearing is plugged in sleeve, and self lubrication bearing is pierced by set The both ends of cylinder are arranged two outer plates, connecting shaft are plugged in self lubrication bearing, the both ends that connecting shaft is pierced by self lubrication bearing cover Equipped with plane bearing and self-locking nut, self-locking nut is threadedly coupled with connecting shaft and plane bearing is pressed on the outside of outer plate;It is flat Surface illuminating device includes light guide plate, LED aluminum base plate and heat dissipation wing plate, and LED aluminum base plate front is provided with a manner of array distribution Multiple red LED lamps and multiple blue LED lamps, the incidence surface of light guide plate are close to aluminium close to the front of aluminum substrate, heat dissipation wing plate The back side of substrate;In the conveying circulation that multiple chains that one planar emission apparatus is arranged on tissue culture bottle conveying device are formed Portion, the conveying circulation outside that multiple chains that another planar emission apparatus is arranged on tissue culture bottle conveying device are formed, this two A planar emission apparatus is defeated to this inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed respectively A vertical plane of circulation is sent to carry out illumination.
Preferably, constant-temperature constant-humidity air-conditioner is installed in the tissue culture transfer room.
Preferably, the vaccination station includes cabinet, the front and rear sides of cabinet are transparent plastic sheet, the left and right sides of cabinet Quartz burner is installed, cabinet inside is equipped with temperature sensor, humidity sensor and welding rifle, cabinet inner wall top on inner wall Equipped with air blowing hole array, cabinet inner wall bottom is equipped with ventilation hole array.
Preferably, the tissue culture transfer room, manually training seedling room and training seedling interior room are all white by aluminum alloy frame and float glass process Glass is built.
Preferably, the tissue culture bottle includes the bottle and bottle cap being threadedly coupled, bottle cap center is equipped with central tube, works as bottle When inside having culture medium, sterile cotton is filled in central tube and by closing of sealing with wax.
Preferably, to the conveying inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed Two planar emission apparatus that one vertical plane of circulation carries out illumination are separately fixed in two mobile bases, the two movements Base is slidably connected with babinet by track level, and the bottom of box sets up a screw, and screw passes through plus thread and left-hand thread respectively Two feed screw nuts are connected with, two feed screw nuts are fixedly connected with two mobile bases respectively, a motor or runner driving silk Bar rotates, so as to drive the conveying circulation that two planar emission apparatus are formed from multiple chains of tissue culture bottle conveying device respectively Inside and outside synchronously closer or far from the conveying circulation the vertical plane by illumination.
Preferably, the red LED lamp and blue LED lamp in the LED aluminum base plate distribute alternately, controller control is each Red LED lamp and blue LED lamp are individually lighted or extinguished.Preferably, the power of the red LED lamp and blue LED lamp is equal For 1W, the red spectral band of red LED lamp is 450 ± 20nm, and the blue wave band of blue LED lamp is 660 ± 20nm.Preferably, The mobile base is equipped with the wind that can promote the circulation circulation of babinet interior air-flow in the heat dissipation wing plate side frames of planar emission apparatus Fan, the ventilation fan that can carry out that air-flow exchanges inside and outside babinet is installed on the babinet.
Technical solution of the present invention as a result of more than, inside sets LED light light incubator, can using planar emission apparatus Conveniently adjust light quality ratio, intensity of illumination and the periodicity of illumination of red blue mixed light.Connect by Airshower chamber into tissue culture Kind room and artificial training seedling room ensure that the cleanliness factor of tissue culture room, by setting artificial training seedling room to ensure that tissue culture room with training seedling interior room Interior temperature and humidity, sets sunshade net and LED light light incubator, dendrobium candidum group is ensure that by natural light and artificial light Training seedling germination, growth and the illumination of hardening needs, in this way, the survival rate of dendrobium candidum can not only be greatly improved, and saves The time cultivated, cultivation cost is reduced, improve economic benefit.
Brief description of the drawings
Fig. 1 is the structure diagram of tissue culture system of the present invention;
Fig. 2 is the structure diagram of LED light light incubator;
Fig. 3 is the partial structural diagram of more chain conveying structures;
Fig. 4 is the cooperation schematic diagram of tissue culture bottle, loop bracket and link unit;
Fig. 5 is the moving structure schematic diagram of planar emission apparatus;
Fig. 6 is the aeration structure schematic diagram of planar emission apparatus.
Embodiment
The embodiment of the present invention is described below in detail, the example of the embodiment is shown in the drawings, wherein from beginning to end Same or similar label represents same or similar element or has the function of same or like element.Below with reference to attached The embodiment of figure description is exemplary, it is intended to for explaining the present invention, and is not considered as limiting the invention.
In the description of the present invention, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " length ", " width ", " thickness ", " on ", " under ", "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom " " interior ", " clockwise ", " inverse The orientation or position relationship of the instructions such as hour hands " are based on orientation shown in the drawings or position relationship, are for only for ease of description originally Invent and simplify description, rather than the device or element of instruction or hint meaning there must be specific orientation, with specific side Position construction and operation, therefore be not considered as limiting the invention.
Tissue culture system as shown in Figure 1, including totally enclosed tissue culture transfer room 2 and artificial training seedling room 3, the tissue culture connect Kind of room 2 and manually training seedling room 3 or so is disposed adjacent, tissue culture transfer room 2 and manually trains the entrance of seedling room 3 and goes out with Airshower chamber 1 Mouth connection, the tissue culture transfer room 2 is interior to set multiple vaccination stations 21 and a conveyer belt 22, the output terminal of conveyer belt 22 to stretch into people In work training seedling room 3, training seedling interior room 4 is equipped with the manually training seedling room 3, if manually between training seedling room 3 and training seedling interior room 4 only by Dry ventilation window ventilating air, enters training seedling interior room 4 from artificial training seedling room 3 by a sliding door, perseverance is equipped with training seedling interior room 4 Constant temperature and humidity air-conditioning and multiple LED light light incubators 41;Be provided with the wall of the manually training seedling room 3 with it is exterior connect it is some A ventilation fan 31.Manually the outside of training seedling room 3 is provided with steel pipe framework, is laid with sunshade net at the top of steel pipe framework, sunshade net passes through Drawstring furls and expansion, and the pulley for drawstring traction is provided with steel pipe framework.Constant temperature is installed in the tissue culture transfer room 2 Constant wetting air conditioner.The vaccination station 21 includes cabinet, and the front and rear sides of cabinet are transparent plastic sheet, on the left and right sides inner wall of cabinet Quartz burner is installed, cabinet inside is equipped with temperature sensor, humidity sensor and welding rifle, is equipped with and blows at the top of cabinet inner wall Stomata array, cabinet inner wall bottom are equipped with ventilation hole array.The tissue culture transfer room 2, manually training seedling room 3 and training seedling interior room 4 are all It is to be built by aluminum alloy frame and float glass process white glass.Tissue culture transfer room is entered by Airshower chamber and manually training seedling room ensure that The cleanliness factor of tissue culture room, by setting manually training seedling room and training seedling interior room to ensure that the indoor temperature and humidity of tissue culture, sets and hides Screened postive and LED light light incubator, candidum tissue culturing seedling germination, growth and hardening are ensure that by natural light and artificial light Illumination needs, in this way, the survival rate of dendrobium candidum can not only be greatly improved, and has saved the time cultivated, has reduced training This is bred as, improves economic benefit.
As shown in Fig. 2, the LED light light incubator includes babinet 411, tissue culture bottle conveying dress is set respectively in babinet 411 Put and multiple planar emission apparatus 412;Tissue culture bottle conveying device includes multiple chains 414, and each chain 414 is set by sprocket wheel In babinet 411 and head and the tail connect the motion cycle being formed in vertical plane, and the motion cycle of multiple chains 414 is parallel to each other simultaneously It is synchronized with the movement by the driving of same driving device, multiple loop brackets that can place tissue culture bottle is set between adjacent chain 414 415, in this way, multiple chains 414 are just formed in the structure for conveying of circulation conveying tissue culture bottle in vertical plane, as shown in Figure 3, Figure 4, ring Shape bracket 415 includes filler ring 4151 and the connecting shaft 4152 being fixedly connected, and is put into tissue culture bottle in filler ring 4151, tissue culture bottle passes through bottle 622 supporting and fixing of flange on 62 circumference of body 61 or bottle cap is in filler ring 4151, the connecting shaft 4152 and chain of loop bracket 415 414 link units connection, the link units include outer plate 4141, inner plate 4142, roller 4143, sleeve 4145 and Zi Run Slide-bar set 4147, two inner plates 4142 are fixedly connected to form inner link by sleeve 4145, roller are arranged outside sleeve 4145 4143, plug self lubrication bearing 4147 in sleeve 4145, self lubrication bearing 4147 be pierced by sleeve 4145 both ends be arranged two it is outer Carrier bar 4141, plugs connecting shaft 4152 in self lubrication bearing 4147, the both ends that connecting shaft 4152 is pierced by self lubrication bearing 4147 are equal It is arranged with plane bearing 4144 and self-locking nut 4146, self-locking nut 4146 is threadedly coupled with connecting shaft 4152 and by plane bearing 4144 are pressed in the outside of outer plate 4141;As shown in figure 5, planar emission apparatus 412 includes light guide plate 4121, LED aluminum base plate 4122 With heat dissipation wing plate 4123, the front of LED aluminum base plate 4122 is provided with multiple red LED lamps and multiple blue lights in a manner of array distribution LED light, the incidence surface of light guide plate 4121 are close to the back of the body of aluminum substrate 4122 close to the front of aluminum substrate 4122, heat dissipation wing plate 4123 Face, is projected after using light guide plate that the light that its bottom LED point light source is sent can be carried out to Mixed adjustment in the form of area source, Uniform illumination and reddish blue light mixes, cost is low;As shown in Fig. 2, to be arranged on tissue culture bottle defeated for a planar emission apparatus 412 Send the conveying circulation that multiple chains of device are formed internal, another planar emission apparatus 412 is arranged on tissue culture bottle conveying dress The conveying circulation outside that the multiple chains put are formed, the two planar emission apparatus 412 are respectively from tissue culture bottle conveying device Illumination is carried out to a vertical plane of conveying circulation inside and outside the conveying circulation that multiple chains are formed.
In the present embodiment, as shown in figure 4, the tissue culture bottle includes the bottle 61 and bottle cap 62 being threadedly coupled, in bottle cap 62 Centre is equipped with central tube 621, when having culture medium in bottle 61, sterile cotton 64 is filled in central tube 621 and by 63 closings of sealing with wax. As shown in figure 5, to conveying circulation inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed Two planar emission apparatus 412 that one vertical plane carries out illumination are separately fixed in two mobile bases 413, the two movements Base 413 is slidably connected with babinet 411 by track level, and 411 bottom of babinet sets up a screw 416, and screw 416 passes through respectively Plus thread and left-hand thread are connected with two feed screw nuts 417, and two feed screw nuts 417 fix with two mobile bases 413 respectively Connection, a motor 418 or runner driving screw 416 rotate, so as to drive two planar emission apparatus 412 defeated from tissue culture bottle respectively Send inside and outside the conveying circulation that multiple chains of device are formed synchronously closer or far from conveying circulation by illumination Vertical plane.So by adjusting the spacing of two planar emission apparatus, it becomes possible to adjust light of the planar emission apparatus to tissue culture face According to intensity, equipment applicability is stronger, and cost is low.Such as controlled using motor, and corresponding photosensitive sensor is set just in babinet It can realize automatically controlling for intensity of illumination.The power of the red LED lamp and blue LED lamp is 1W, red LED lamp it is red Optical band is 450 ± 20nm, and the blue wave band of blue LED lamp is 660 ± 20nm.Red-light LED in the LED aluminum base plate 4122 Lamp and blue LED lamp are distributed alternately with spacing 2cm × 2cm, and controller controls each red LED lamp and the independent point of blue LED lamp It is bright or extinguish, so by controlling lighting quantity and distribution and just can easily adjusting plane for red LED lamp and blue LED lamp The light quality ratio of light-emitting device.The mobile base 413 is equipped with energy in 4123 side frames of heat dissipation wing plate of planar emission apparatus 412 Enough promote the fan 419 of 411 interior air-flow of babinet circulation circulation, outer gas in babinet 411 can be carried out by being provided with the babinet 411 Flow the ventilation fan 420 exchanged.In this way, LED light can not only be helped to radiate, and LED light can be maintained using these heats Temperature in light incubator, when temperature is higher in LED light light incubator, can also radiate to training seedling interior room etc..
Although the embodiment of the present invention has been shown and described above, it is to be understood that above-described embodiment is example Property, it is impossible to limitation of the present invention is interpreted as, those of ordinary skill in the art are not departing from the principle of the present invention and objective In the case of above-described embodiment can be changed within the scope of the invention, change, replace and modification.

Claims (1)

1. the planar emission apparatus for LED light light incubator, it is characterised in that including light guide plate (4121), LED aluminum base plate (4122) and heat dissipation wing plate (4123), LED aluminum base plate (4122) front are provided with multiple red LED lamps in a manner of array distribution With multiple blue LED lamps, close to the front of aluminum substrate (4122), heat dissipation wing plate (4123) is close to for the incidence surface of light guide plate (4121) At the back side of aluminum substrate (4122);Also, a planar emission apparatus (412) is arranged on multiple chains of tissue culture bottle conveying device The conveying circulation formed is internal, another planar emission apparatus (412) is arranged on multiple chain institutes of tissue culture bottle conveying device The conveying circulation outside of formation, the two planar emission apparatus (412) are respectively from multiple chain institutes shape of tissue culture bottle conveying device Into conveying circulation inside and outside to the conveying circulation a vertical plane carry out illumination;From the multiple of tissue culture bottle conveying device Carry out illumination inside and outside the conveying circulation that chain is formed to the vertical plane that the conveying circulates two are flat luminous Device (412) is separately fixed on two mobile bases (413), the two mobile bases (413) pass through track with babinet (411) Level is slidably connected, and babinet (411) bottom sets up a screw (416), and screw (416) is connected by plus thread with left-hand thread respectively There are two feed screw nuts (417), two feed screw nuts (417) are fixedly connected with two mobile bases (413) respectively, a motor (418) or runner driving screw (416) rotates, and is filled so as to drive two planar emission apparatus (412) to be conveyed respectively from tissue culture bottle Synchronously closer or far from conveying circulation by the vertical of illumination inside and outside the conveying circulation that the multiple chains put are formed Face;Red LED lamp and blue LED lamp on the LED aluminum base plate (4122) distribute alternately, and controller controls each red-light LED Lamp and blue LED lamp are individually lighted or extinguished;The power of the red LED lamp and blue LED lamp is 1W, red LED lamp Red spectral band is 450 ± 20nm, and the blue wave band of blue LED lamp is 660 ± 20nm;The tissue culture bottle conveying device includes multiple Chain (414), each chain (414) is arranged on by sprocket wheel in babinet (411) and head and the tail connect the fortune being formed in vertical plane Dynamic circulation, the motion cycle of multiple chains (414) is parallel to each other and is synchronized with the movement by the driving of same driving device, adjacent chain (414) multiple loop brackets (415) that can place tissue culture bottle are set between, in this way, multiple chains (414) are just formed in vertically The structure for conveying of circulation conveying tissue culture bottle in face, loop bracket (415) include filler ring (4151) and the connecting shaft being fixedly connected (4152), tissue culture bottle is put into filler ring (4151), tissue culture bottle is propped up by the flange (622) on bottle (61) or bottle cap (62) circumference Hold and be fixed in filler ring (4151), the connecting shaft (4152) of loop bracket (415) is connected with the link units of chain (414), should Link units include outer plate (4141), inner plate (4142), roller (4143), sleeve (4145) and self lubrication bearing (4147), two inner plates (4142) are fixedly connected to form inner link by sleeve (4145), and roller is arranged outside sleeve (4145) (4143), self lubrication bearing (4147) is plugged in sleeve (4145), self lubrication bearing (4147) is pierced by the both ends of sleeve (4145) Two outer plates (4141) are arranged, connecting shaft (4152) is plugged in self lubrication bearing (4147), connecting shaft (4152) is pierced by from profit The both ends of slide-bar set (4147) are arranged with plane bearing (4144) and self-locking nut (4146), and self-locking nut (4146) is with being connected Axis (4152) is threadedly coupled and plane bearing (4144) is pressed on the outside of outer plate (4141);Tissue culture bottle includes the bottle being threadedly coupled Body (61) and bottle cap (62), bottle cap (62) center are equipped with central tube (621), when having culture medium in bottle (61), central tube (621) sterile cotton (64) is filled in and by (63) closing of sealing with wax.
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