CN104855291B - Tissue culture system - Google Patents
Tissue culture system Download PDFInfo
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- CN104855291B CN104855291B CN201510299391.6A CN201510299391A CN104855291B CN 104855291 B CN104855291 B CN 104855291B CN 201510299391 A CN201510299391 A CN 201510299391A CN 104855291 B CN104855291 B CN 104855291B
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- training seedling
- bottle
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- 238000012549 training Methods 0.000 claims abstract description 52
- 238000005286 illumination Methods 0.000 claims abstract description 22
- 238000012546 transfer Methods 0.000 claims abstract description 19
- 230000033001 locomotion Effects 0.000 claims abstract description 13
- 239000000945 filler Substances 0.000 claims abstract description 11
- 230000001360 synchronised effect Effects 0.000 claims abstract description 6
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 15
- 229910052782 aluminium Inorganic materials 0.000 claims description 15
- 238000005461 lubrication Methods 0.000 claims description 12
- 238000009423 ventilation Methods 0.000 claims description 11
- 229910000831 Steel Inorganic materials 0.000 claims description 9
- 239000010959 steel Substances 0.000 claims description 9
- 239000004411 aluminium Substances 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 238000009826 distribution Methods 0.000 claims description 4
- 229910000838 Al alloy Inorganic materials 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 238000006124 Pilkington process Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 239000002985 plastic film Substances 0.000 claims description 3
- 239000010453 quartz Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 238000003466 welding Methods 0.000 claims description 3
- 238000007664 blowing Methods 0.000 claims description 2
- 230000003595 spectral effect Effects 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000005096 rolling process Methods 0.000 claims 1
- 241000026010 Dendrobium candidum Species 0.000 description 12
- 230000012010 growth Effects 0.000 description 5
- 241001076416 Dendrobium tosaense Species 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
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- 230000035784 germination Effects 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 241001523681 Dendrobium Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000003749 cleanliness Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012869 germination medium Substances 0.000 description 1
- 231100000753 hepatic injury Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 238000011160 research Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a kind of tissue culture system, including totally enclosed tissue culture transfer room and artificial training seedling room, multiple LED light incubators are provided with artificial training seedling room；LED light incubator includes setting tissue culture bottle conveying device and multiple planar emission apparatus respectively in casing, casing；Tissue culture bottle conveying device includes multiple chains, the motion cycle of multiple chains is parallel to each other and is synchronized with the movement by the driving of same drive device, being set between adjacent chain multiple can place the loop bracket of tissue culture bottle, loop bracket includes filler ring and the connecting shaft being fixedly connected, tissue culture bottle is put into filler ring, one planar emission apparatus is arranged on the conveying circulation inside that multiple chains are formed, another planar emission apparatus is arranged on the conveying circulation outside that multiple chains are formed, and the two planar emission apparatus carry out illumination from the vertical plane circulated to the conveying respectively.This programme can conveniently adjust light quality ratio, intensity of illumination and the periodicity of illumination of red blue mixed light.
Description
Technical field
The present invention relates to a kind of tissue culture system for plants such as dendrobium candidums.
Background technology
Dendrobium candidum, is one of kind the most precious in China's orchid family Dendrobium Sw.Because there is enhancing body to exempt from for it
Epidemic disease power, antitumor, anti-aging and the effectively effect such as mitigation hepatic injury, in version in 2010《Pharmacopoeia of People's Republic of China》Middle coverlet
Medicinal material simply solely is classified as, due to artificial excessively excavation and the destruction of ecological environment, its own low reproduction rate, wild iron sheet stone in addition
Dry measure used in former times resource is increasingly exhausted, is classified as the rare or endangered species of two grades of focused protections by country.Now, artificial cultivation plantation iron sheet stone
Dry measure used in former times turns into the main path for meeting great market demand.Dendrobium candidum system orchid family Dendrobium Sw, seed is minimum, without endosperm,
Need to sprout with some mycosymbiosises under natural conditions, germination percentage only a ten thousandth in the case of nature, and cultivar is present
Degradation phenomena.
In recent years, some scientific research institutions and business unit of China are carried out to the tissue culture and artificial cultivation technique of dendrobium candidum
Substantial amounts of exploration and experiment, achieve some achievements, also make it that artificial cultivation dendrobium candidum yield increased in recent years.But
It is that the artificial culture of dendrobium candidum still has slow seeding propagation speed, training tissue culture seedling and transplanting survival rate is low, artificial growth
The problem of product active constituent content is low, seriously constrains industrialization, the large-scale development of dendrobium candidum.In addition, dendrobium candidum
General field planting can be with growth of weeds, and be vulnerable to pest and disease damage.
The light that Publication No. CN102057867A patent document discloses a kind of tissue cultures of seedlings of Dendrobium officinale is adjusted
Prosecutor method, including by Dendrobium officinale fruit sterilize after obtain powdered seed, powdered seed is trained by seed germination medium
Support, sprout the process for forming protocorm, it is characterised in that in the tissue culture procedures of seedlings of Dendrobium officinale, (semiconductor is sent out with LED
Optical diode) plant growth lighting system is as light source, according to the different phase of dendrobium candidum tissue culture, the different light of implementation
According to processing.Implement different photo-irradiation treatments by using different growth phases, seedlings of Dendrobium officinale is maximally utilised light
Its photosynthesis can be supplied, so as to obtain maximum growth amount.
In the tissue culture system of prior art, it is impossible to conveniently and efficiently adjust light quality ratio, the intensity of illumination of red blue mixed light
And periodicity of illumination.
The content of the invention
In order to solve above-mentioned technical problem, it is an object of the invention to provide a kind of tissue culture system, LED optical culture is inside set
Case, light quality ratio, intensity of illumination and the illumination week of red blue mixed light can be conveniently adjusted using planar emission apparatus
Phase.
To achieve the above object, present invention employs following technical scheme：
A kind of tissue culture system, including totally enclosed tissue culture transfer room and artificial training seedling room, described tissue culture transfer room and people
Work training seedling room or so is disposed adjacent, and the entrance of tissue culture transfer room and artificial training seedling room is connected with the outlet of Airshower chamber, described group
Train and multiple inoculation platforms and a conveyer belt are set in transfer room, the output end of conveyer belt is stretched into artificial training seedling room, described artificial
Train provided with training seedling interior room in seedling room, only by several ventilation window ventilating airs between artificial training seedling room and training seedling interior room, from
Artificial training seedling room is entered by a sliding door trains seedling interior room, indoor in training seedling to be trained provided with constant-temperature constant-humidity air-conditioner and multiple LED lamplights
Support case；Several ventilation fans with ft connection are installed, the outside peace of artificial training seedling room on the wall of the artificial training seedling room
Equipped with steel framework, sunshade net is laid at the top of steel framework, sunshade net is furled and deployed by drawstring, is provided with steel framework
For the pulley of drawstring traction；The LED light incubator includes setting tissue culture bottle conveying device in casing, casing respectively and many
Individual planar emission apparatus；Tissue culture bottle conveying device includes multiple chains, and each chain is arranged in casing and head and the tail by sprocket wheel
Connection forms the motion cycle in vertical plane, and the motion cycle of multiple chains is parallel to each other and is driven by same drive device same
Step motion, set between adjacent chain it is multiple can place the loop bracket of tissue culture bottle, so, multiple chains are just formed vertical
Face interior circulation conveys the structure for conveying of tissue culture bottle, and loop bracket includes being put into group in filler ring and the connecting shaft being fixedly connected, filler ring
Bottle is trained, tissue culture bottle is fixed in filler ring by the flange bearing on bottle or bottle cap circumference, the connecting shaft and chain of loop bracket
Link units connection, the link units include outer plate, inner plate, roller, sleeve and self lubrication bearing, and two inner plates lead to
Cross sleeve and be fixedly connected to form inner link, bush outer sleeve sets and self lubrication bearing is plugged in roller, sleeve, and self lubrication bearing passes set
The two ends of cylinder, which are arranged in two outer plates, self lubrication bearing, plugs connecting shaft, and the two ends that connecting shaft passes self lubrication bearing cover
Provided with plane bearing and self-locking nut, self-locking nut is threadedly coupled with connecting shaft and plane bearing is pressed on the outside of outer plate；It is flat
Surface illuminating device includes light guide plate, LED aluminum base plate and radiating fin plate, and LED aluminum base plate front is provided with the way of array distribution
Multiple red LED lamps and multiple blue LED lamps, the incidence surface of light guide plate press close to the front of aluminium base, and radiating fin plate is close to aluminium
The back side of substrate；In the conveying circulation that multiple chains that one planar emission apparatus is arranged on tissue culture bottle conveying device are formed
Portion, the conveying circulation outside that multiple chains that another planar emission apparatus is arranged on tissue culture bottle conveying device are formed, this two
Individual planar emission apparatus is defeated to this inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed respectively
A vertical plane of circulation is sent to carry out illumination.
Preferably, being provided with constant-temperature constant-humidity air-conditioner in the tissue culture transfer room.
Preferably, the inoculation platform includes cabinet, the front and rear sides of cabinet are transparent plastic sheet, the left and right sides of cabinet
Quartz burner is installed, cabinet inside is provided with temperature sensor, humidity sensor and welding rifle, cabinet inwall top on inwall
Provided with air blowing hole array, cabinet inwall bottom is provided with ventilation hole array.
Preferably, the tissue culture transfer room, artificial training seedling room and training seedling interior room are all white by aluminum alloy frame and float glass process
Glass is built.
Preferably, the tissue culture bottle includes the bottle and bottle cap of threaded connection, bottle cap center is provided with central tube, works as bottle
Sterile cotton is filled in when inside having culture medium, in central tube and by closing of sealing with wax.
Preferably, to the conveying inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed
Two planar emission apparatus that one vertical plane of circulation carries out illumination are separately fixed in two mobile bases, the two movements
Base is slidably connected with casing by track level, and bottom half sets up a screw mandrel, and screw mandrel passes through plus thread and left-hand thread respectively
Two feed screw nuts are connected with, two feed screw nuts are fixedly connected with two mobile bases respectively, a motor or runner driving silk
Bar is rotated, so as to drive the conveying circulation that two planar emission apparatus are formed from multiple chains of tissue culture bottle conveying device respectively
Inside and outside the synchronous vertical plane by illumination circulated closer or far from the conveying.
Preferably, the red LED lamp and blue LED lamp in the LED aluminum base plate distribute alternately, controller control is each
Red LED lamp and blue LED lamp are individually lighted or extinguished.Preferably, the power of the red LED lamp and blue LED lamp is equal
For 1W, the red spectral band of red LED lamp is 450 ± 20nm, and the blue wave band of blue LED lamp is 660 ± 20nm.Preferably,
The mobile base is provided with the radiating fin plate side frames of planar emission apparatus can promote the wind of casing interior air-flow circulation circulation
The ventilation fan that can carry out that air-flow is exchanged inside and outside casing is installed on fan, the casing.
Technical scheme of the present invention as a result of more than, inside sets LED light incubator, can using planar emission apparatus
Conveniently adjust light quality ratio, intensity of illumination and the periodicity of illumination of red blue mixed light.Connect by Airshower chamber into tissue culture
Plant room and artificial training seedling room ensure that the cleanliness factor of tissue culture room, by setting manually training seedling room and training seedling interior room to ensure that tissue culture room
Interior temperature and humidity, sets sunshade net and LED light incubator, dendrobium candidum group is ensure that by natural light and artificial light
Training seedling germination, growth and the illumination of hardening needs, so, can not only greatly improve the survival rate of dendrobium candidum, and save
The time cultivated, cultivation cost is reduced, improve economic benefit.
Brief description of the drawings
Fig. 1 is the structural representation of tissue culture system of the present invention；
Fig. 2 is the structural representation of LED light incubator；
Fig. 3 is the partial structural diagram of many chain conveying structures；
Fig. 4 is the cooperation schematic diagram of tissue culture bottle, loop bracket and link unit；
Fig. 5 is the moving structure schematic diagram of planar emission apparatus；
Fig. 6 is the aeration structure schematic diagram of planar emission apparatus.
Embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is shown in the drawings, wherein from beginning to end
Same or similar label represents same or similar element or the element with same or like function.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to for explaining the present invention, and be not considered as limiting the invention.
In the description of the invention, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", " on ", " under ", "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom " " interior ", " clockwise ", " inverse
The orientation or position relationship of the instructions such as hour hands " are, based on orientation shown in the drawings or position relationship, to be for only for ease of description originally
Invention and simplified description, rather than instruction or the signified device of hint or element must be with specific orientation, with specific square
Position construction and operation, therefore be not considered as limiting the invention.
Tissue culture system as shown in Figure 1, including totally enclosed tissue culture transfer room 2 and artificial training seedling room 3, described tissue culture connect
Kind of room 2 and artificial training seedling room 3 or so are disposed adjacent, and tissue culture transfer room 2 and are manually trained the entrance of seedling room 3 and are gone out with Airshower chamber 1
Multiple inoculation platforms 21 and a conveyer belt 22 are set in mouth connection, the tissue culture transfer room 2, the output end of conveyer belt 22 stretches into people
In work training seedling room 3, provided with training seedling interior room 4 in the artificial training seedling room 3, if manually between training seedling room 3 and training seedling interior room 4 only by
Dry ventilation window ventilating air, is entered in training seedling interior room 4, training seedling interior room 4 provided with perseverance from artificial training seedling room 3 by a sliding door
Constant temperature and humidity air-conditioning and multiple LED light incubators 41；It is provided with the wall of the artificial training seedling room 3 some with ft connection
Individual ventilation fan 31.The outside of artificial training seedling room 3 is provided with laying sunshade net at the top of steel framework, steel framework, and sunshade net passes through
Drawstring is furled and deployed, and the pulley for drawstring traction is provided with steel framework.Constant temperature is installed in the tissue culture transfer room 2
Constant wetting air conditioner.The inoculation platform 21 includes cabinet, and the front and rear sides of cabinet are on transparent plastic sheet, the left and right sides inwall of cabinet
Quartz burner is installed, cabinet inside is provided with temperature sensor, humidity sensor and welding rifle, and cabinet inwall top, which is provided with, blows
Stomata array, cabinet inwall bottom is provided with ventilation hole array.The tissue culture transfer room 2, artificial training seedling room 3 and training seedling interior room 4 are all
It is to be built by aluminum alloy frame and float glass process white glass.Tissue culture transfer room is entered by Airshower chamber and artificial training seedling room ensure that
The cleanliness factor of tissue culture room, by setting artificial training seedling room and training seedling interior room to ensure that the temperature and humidity in tissue culture room, sets and hides
Screened postive and LED light incubator, candidum tissue culturing seedling germination, growth and hardening are ensure that by natural light and artificial light
Illumination needs, and so, can not only greatly improve the survival rate of dendrobium candidum, and has saved the time cultivated, and reduces training
This is bred as, economic benefit is improved.
As shown in Fig. 2 the LED light incubator includes setting tissue culture bottle conveying dress in casing 411, casing 411 respectively
Put and multiple planar emission apparatus 412；Tissue culture bottle conveying device includes multiple chains 414, and each chain 414 is set by sprocket wheel
Simultaneously head and the tail connect the motion cycle formed in vertical plane in casing 411, and the motion cycle of multiple chains 414 is parallel to each other simultaneously
It is synchronized with the movement by the driving of same drive device, being set between adjacent chain 414 multiple can place the loop bracket of tissue culture bottle
415, so, multiple chains 414 just form the structure for conveying that tissue culture bottle is conveyed in vertical plane interior circulation, as shown in Figure 3, Figure 4, ring
Shape bracket 415 includes being put into tissue culture bottle in filler ring 4151 and the connecting shaft 4152 being fixedly connected, filler ring 4151, and tissue culture bottle passes through bottle
The supporting and fixing of flange 622 on body 61 or the circumference of bottle cap 62 is in filler ring 4151, the connecting shaft 4152 and chain of loop bracket 415
414 link units connection, the link units include outer plate 4141, inner plate 4142, roller 4143, sleeve 4145 and Zi Run
Slide-bar set 4147, two inner plates 4142 are fixedly connected to form outside inner link, sleeve 4145 by sleeve 4145 and are arranged roller
4143, plug self lubrication bearing 4147 in sleeve 4145, self lubrication bearing 4147 pass sleeve 4145 two ends be arranged two it is outer
Connecting shaft 4152 is plugged in carrier bar 4141, self lubrication bearing 4147, the two ends that connecting shaft 4152 passes self lubrication bearing 4147 are equal
It is arranged with plane bearing 4144 and self-locking nut 4146, self-locking nut 4146 is threadedly coupled with connecting shaft 4152 and by plane bearing
4144 are pressed in the outside of outer plate 4141；As shown in figure 5, planar emission apparatus 412 includes light guide plate 4121, LED aluminum base plate 4122
With radiating fin plate 4123, the front of LED aluminum base plate 4122 is provided with multiple red LED lamps and multiple blue lights in the way of array distribution
LED, the incidence surface of light guide plate 4121 presses close to the front of aluminium base 4122, and radiating fin plate 4123 is close to the back of the body of aluminium base 4122
Face, is projected in the form of light that light guide plate can send its bottom LED point light source carries out after Mixed adjustment with area source,
Uniform illumination and reddish blue light is mixed, cost is low；As shown in Fig. 2 to be arranged on tissue culture bottle defeated for a planar emission apparatus 412
The conveying circulation for sending multiple chains of device to be formed is internal, and another planar emission apparatus 412 is arranged on tissue culture bottle conveying dress
The conveying circulation outside that the multiple chains put are formed, the two planar emission apparatus 412 are respectively from tissue culture bottle conveying device
Illumination is carried out to the vertical plane that the conveying is circulated inside and outside the conveying circulation that multiple chains are formed.
In the present embodiment, as shown in figure 4, the tissue culture bottle is included in the bottle 61 and bottle cap 62 of threaded connection, bottle cap 62
Centre is provided with central tube 621, when having culture medium in bottle 61, sterile cotton 64 is filled in central tube 621 and by 63 closings of sealing with wax.
As shown in figure 5, the conveying is circulated inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed
Two planar emission apparatus 412 that one vertical plane carries out illumination are separately fixed in two mobile bases 413, the two movements
Base 413 is slidably connected with casing 411 by track level, and the bottom of casing 411 sets up a screw mandrel 416, and screw mandrel 416 passes through respectively
Plus thread and left-hand thread are connected with two feed screw nuts 417, and two feed screw nuts 417 fix with two mobile bases 413 respectively
Connection, a motor 418 or runner drive screw 416 are rotated, so as to drive two planar emission apparatus 412 defeated from tissue culture bottle respectively
Send inside and outside the conveying circulation that multiple chains of device are formed it is synchronous closer or far from the conveying circulate by illumination
Vertical plane.Spacing so by adjusting two planar emission apparatus, it becomes possible to adjust light of the planar emission apparatus to tissue culture face
According to intensity, equipment applicability is stronger, and cost is low.Motor control is such as used, and corresponding light sensor is set just in casing
Automatically controlling for intensity of illumination can be realized.The power of the red LED lamp and blue LED lamp is 1W, red LED lamp it is red
Optical band is 450 ± 20nm, and the blue wave band of blue LED lamp is 660 ± 20nm.Red-light LED in the LED aluminum base plate 4122
Lamp and blue LED lamp are distributed alternately with spacing 2cm × 2cm, and controller controls each red LED lamp and the independent point of blue LED lamp
It is bright or extinguish, so by controlling lighting quantity and distribution and just can easily adjusting plane for red LED lamp and blue LED lamp
The light quality ratio of light-emitting device.The mobile base 413 is provided with energy in the side frames of radiating fin plate 4123 of planar emission apparatus 412
Enough promote to be provided with the fan 419 of the interior air-flow of casing 411 circulation circulation, the casing 411 and can carry out outer gas in casing 411
Flow the ventilation fan 420 exchanged.So, LED can not only be helped to radiate, and LED can be maintained using these heats
Temperature in light incubator, when temperature is higher in LED light incubator, can also radiate to training seedling interior room etc..
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art is not departing from the principle and objective of the present invention
In the case of above-described embodiment can be changed within the scope of the invention, change, replace and modification.
Claims (7)
1. a kind of tissue culture system, it is characterised in that described including totally enclosed tissue culture transfer room (2) and artificial training seedling room (3)
Tissue culture transfer room (2) and artificial training seedling room (3) left and right are disposed adjacent, and the entrance of tissue culture transfer room (2) and artificial training seedling room (3) is equal
Outlet with Airshower chamber (1) is connected, and multiple inoculation platforms (21) and a conveyer belt (22) are set in the tissue culture transfer room (2),
The output end of conveyer belt (22) is stretched into artificial training seedling room (3), provided with training seedling interior room (4) in the artificial training seedling room (3), manually
Train between seedling room (3) and training seedling interior room (4) only by several ventilation window ventilating airs, one is passed through from artificial training seedling room (3)
Individual sliding door, which enters in training seedling interior room (4), training seedling interior room (4), is provided with constant-temperature constant-humidity air-conditioner and multiple LED light incubators (41)；Institute
Several ventilation fans (31) with ft connection are installed on the wall for stating artificial training seedling room (3), the outside of seedling room (3) is manually trained
Steel framework (5) is installed, laying sunshade net (51) at the top of steel framework (5), sunshade net (51) by drawstring (52) retracting and
Pulley for drawstring (52) traction is installed on expansion, steel framework (5)；The LED light incubator includes casing
(411) tissue culture bottle conveying device and multiple planar emission apparatus (412), are set respectively in casing (411)；Tissue culture bottle conveying device
Including multiple chains (414), each chain (414) is arranged in casing (411) by sprocket wheel and head and the tail are connected and formed vertical
Motion cycle in face, the motion cycle of multiple chains (414) is parallel to each other and is synchronized with the movement by the driving of same drive device, phase
Multiple loop brackets (415) that can place tissue culture bottle are set between adjacent chain (414), and so, multiple chains (414) are just formed
The structure for conveying of tissue culture bottle is conveyed in vertical plane interior circulation, loop bracket (415) includes the filler ring (4151) being fixedly connected and connected
Tissue culture bottle is put into spindle (4152), filler ring (4151), tissue culture bottle passes through the flange on bottle (61) or bottle cap (62) circumference
(622) supporting and fixing is in filler ring (4151), the connecting shaft (4152) of loop bracket (415) and the link units of chain (414)
Connection, the link units include outer plate (4141), inner plate (4142), roller (4143), sleeve (4145) and self-lubricating axle
Cover (4147), two inner plates (4142) are fixedly connected to form outside inner link, sleeve (4145) by sleeve (4145) and are arranged rolling
Self lubrication bearing (4147) is plugged in sub (4143), sleeve (4145), self lubrication bearing (4147) passes the two of sleeve (4145)
End, which is arranged in two outer plates (4141), self lubrication bearing (4147), plugs connecting shaft (4152), and connecting shaft (4152) is passed certainly
The two ends of lubricative axle sleeve (4147) are arranged with plane bearing (4144) and self-locking nut (4146), and self-locking nut (4146) is with connecting
Spindle (4152) is threadedly coupled and plane bearing (4144) is pressed on the outside of outer plate (4141)；Planar emission apparatus (412) is wrapped
Light guide plate (4121), LED aluminum base plate (4122) and radiating fin plate (4123) are included, LED aluminum base plate (4122) front is with array distribution
Mode be provided with multiple red LED lamps and multiple blue LED lamps, the incidence surface of light guide plate (4121) presses close to aluminium base (4122)
Front, radiating fin plate (4123) is close to the back side of aluminium base (4122)；One planar emission apparatus (412) is arranged on tissue culture
The conveying circulation that multiple chains of bottle conveying device are formed is internal, and another planar emission apparatus (412) is arranged on tissue culture bottle
The conveying circulation outside that multiple chains of conveying device are formed, the two planar emission apparatus (412) are defeated from tissue culture bottle respectively
The conveying for sending multiple chains of device to be formed carries out illumination inside and outside circulating to the vertical plane that the conveying is circulated；Institute
State in tissue culture transfer room (2) and constant-temperature constant-humidity air-conditioner is installed；The tissue culture transfer room (2), manually training seedling room (3) and training seedling interior room
(4) all it is to be built by aluminum alloy frame and float glass process white glass.
2. a kind of tissue culture system according to claim 1, it is characterised in that the inoculation platform (21) includes cabinet, cabinet
Front and rear sides be transparent plastic sheet, quartz burner is installed, cabinet inside is provided with temperature on the left and right sides inwall of cabinet
Air blowing hole array is provided with the top of sensor, humidity sensor and welding rifle, cabinet inwall, cabinet inwall bottom is provided with passage battle array
Row.
3. a kind of tissue culture system according to claim 1, it is characterised in that the tissue culture bottle includes the bottle of threaded connection
(61) and bottle cap (62), bottle cap (62) center is provided with central tube (621), when having culture medium in bottle (61), central tube (621)
Inside fill in sterile cotton (64) and by (63) closing of sealing with wax.
4. a kind of tissue culture system according to claim 1, it is characterised in that the feux rouges on the LED aluminum base plate (4122)
LED and blue LED lamp distribute alternately, and controller controls each red LED lamp and blue LED lamp individually to light or extinguish.
5. a kind of tissue culture system according to claim 1, it is characterised in that the work(of the red LED lamp and blue LED lamp
Rate is 1W, and the red spectral band of red LED lamp is 450 ± 20nm, and the blue wave band of blue LED lamp is 660 ± 20nm.
6. a kind of tissue culture system according to claim 1, it is characterised in that from multiple chain institutes of tissue culture bottle conveying device
Two planar emission apparatus of illumination are carried out inside and outside the conveying circulation of formation to the vertical plane that the conveying is circulated
(412) it is separately fixed on two mobile bases (413), the two mobile bases (413) pass through track level with casing (411)
It is slidably connected, casing (411) bottom sets up a screw mandrel (416), screw mandrel (416) is connected with two by plus thread and left-hand thread respectively
Individual feed screw nut (417), two feed screw nuts (417) are fixedly connected with two mobile bases (413) respectively, a motor (418)
Or runner drive screw (416) is rotated, so as to drive two planar emission apparatus (412) respectively from many of tissue culture bottle conveying device
The synchronous vertical plane by illumination circulated closer or far from the conveying inside and outside the conveying circulation that individual chain is formed.
7. a kind of tissue culture system according to claim 6, it is characterised in that mobile base (413) is in planar emission apparatus
(412) radiating fin plate (4123) side frames, which are provided with, can promote the fan (419) of casing (411) interior air-flow circulation circulation, institute
State the ventilation fan (420) for being provided with casing (411) and can carrying out that air-flow is exchanged inside and outside casing (411).
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610472548.5A CN105993957B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its led light incubator |
| CN201510299391.6A CN104855291B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system |
| CN201610472573.3A CN105918135B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its tissue culture bottle conveying device, LED light light incubator |
| CN201610442545.7A CN106035089B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its manually train seedling room |
| CN201610442519.4A CN106106146B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its planar emission apparatus, LED light light incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510299391.6A CN104855291B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system |
Related Child Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610472548.5A Division CN105993957B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its led light incubator |
| CN201610442545.7A Division CN106035089B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its manually train seedling room |
| CN201610472573.3A Division CN105918135B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its tissue culture bottle conveying device, LED light light incubator |
| CN201610442519.4A Division CN106106146B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its planar emission apparatus, LED light light incubator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104855291A CN104855291A (en) | 2015-08-26 |
| CN104855291B true CN104855291B (en) | 2017-11-07 |
Family
ID=53901823
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610472573.3A Expired - Fee Related CN105918135B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its tissue culture bottle conveying device, LED light light incubator |
| CN201510299391.6A Expired - Fee Related CN104855291B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system |
| CN201610442545.7A Expired - Fee Related CN106035089B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its manually train seedling room |
| CN201610472548.5A Expired - Fee Related CN105993957B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its led light incubator |
| CN201610442519.4A Active CN106106146B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its planar emission apparatus, LED light light incubator |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610472573.3A Expired - Fee Related CN105918135B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its tissue culture bottle conveying device, LED light light incubator |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610442545.7A Expired - Fee Related CN106035089B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its manually train seedling room |
| CN201610472548.5A Expired - Fee Related CN105993957B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its led light incubator |
| CN201610442519.4A Active CN106106146B (en) | 2015-06-04 | 2015-06-04 | Tissue culture system and its planar emission apparatus, LED light light incubator |
Country Status (1)
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|---|---|
| CN (5) | CN105918135B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123488B (en) * | 2015-08-28 | 2018-05-15 | 上海离草科技有限公司 | Plant tissue culture is taken root, the integrated culture apparatus of hardening and its cultural method |
| CN107101121B (en) * | 2017-06-14 | 2023-09-29 | 中国科学院工程热物理研究所 | LED device |
| CN110339871A (en) * | 2019-08-12 | 2019-10-18 | 大连天成嘉诺生物科技有限公司 | A kind of biotechnology exploitation multilayer propelling movement type constant temperature and humidity incubator |
| CN111011213A (en) * | 2019-12-29 | 2020-04-17 | 金华匠康金草生态农业科技有限公司 | Anoectochilus roxburghii culture method for improving drying rate |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1197439A (en) * | 1995-09-05 | 1998-10-28 | 欧罗普尔公司 | Device for suspended transport of containers possessing protruding collars |
| JPH1066464A (en) * | 1997-08-05 | 1998-03-10 | Tabai Espec Corp | Culture system for plant tissue culture seedling |
| CN101310591A (en) * | 2008-04-23 | 2008-11-26 | 孙以川 | Modern system agricultural industry system designed according to material and energy cycle |
| CN201225533Y (en) * | 2008-05-26 | 2009-04-22 | 中国农业科学院农业环境与可持续发展研究所 | Tissue culture used tubular LED light source device |
| CN201221695Y (en) * | 2008-05-28 | 2009-04-15 | 中国农业科学院农业环境与可持续发展研究所 | LED light source device aiming at tissue culture container |
| CN102057867B (en) * | 2009-11-14 | 2012-07-18 | 浙江农林大学 | Light control method for dendrobium officinale tissue culture |
| CN201762311U (en) * | 2010-08-13 | 2011-03-16 | 洛阳鸿安生化科技有限公司 | Germ culturing device |
| CN201932637U (en) * | 2011-01-06 | 2011-08-17 | 长安大学 | Chained vertical elevator |
| CN103486493A (en) * | 2012-06-13 | 2014-01-01 | 普罗斯电器（中国）有限公司 | Integrally packaged LED (light emitting diode) plant lighting module |
| CN103662627A (en) * | 2012-09-03 | 2014-03-26 | 仪征市福斯特机械制造有限责任公司 | Conveyor chain mechanism |
| CN202880308U (en) * | 2012-09-29 | 2013-04-17 | 江苏通达机械设备制造有限公司 | Chain-type elevator |
| US20140273197A1 (en) * | 2013-03-14 | 2014-09-18 | Cashido Corporation | Vibratable culture apparatus for providing plant cells with growth environment |
| JP6325771B2 (en) * | 2013-03-18 | 2018-05-16 | スタンレー電気株式会社 | Cultivation method using plant growing lighting device and plant growing lighting device |
| CN203498392U (en) * | 2013-09-09 | 2014-03-26 | 广西大学 | Lifting liquid intermittent-impregnation automatic-cyclic culture device |
| CN103651129A (en) * | 2013-11-26 | 2014-03-26 | 大连好地农业有限公司 | Acclimatization system of tissue culture seedlings |
| CN103891611B (en) * | 2014-01-25 | 2016-05-25 | 杭州峻鸣科技有限公司 | A kind of plant tissue culture bottle with LED light source |
| CN204014697U (en) * | 2014-01-25 | 2014-12-17 | 杭州峻鸣科技有限公司 | A kind of plant tissue culture bottle with LED light source |
| CN104014697B (en) * | 2014-05-20 | 2016-06-01 | 芜湖卓越线束***有限公司 | Wire harness cutting device |
| CN203912830U (en) * | 2014-05-29 | 2014-11-05 | 江苏益草堂石斛股份有限公司 | A kind of novel group of training chamber that utilizes natural daylight to cultivate dendrobium candidum |
| CN204047473U (en) * | 2014-10-14 | 2014-12-31 | 淮北师范大学 | A kind of incubator for tissue culture of Plant Tissue Breeding |
| CN204742126U (en) * | 2015-06-04 | 2015-11-11 | 陆培玉 | System of banking up with earth of group |
-
2015
- 2015-06-04 CN CN201610472573.3A patent/CN105918135B/en not_active Expired - Fee Related
- 2015-06-04 CN CN201510299391.6A patent/CN104855291B/en not_active Expired - Fee Related
- 2015-06-04 CN CN201610442545.7A patent/CN106035089B/en not_active Expired - Fee Related
- 2015-06-04 CN CN201610472548.5A patent/CN105993957B/en not_active Expired - Fee Related
- 2015-06-04 CN CN201610442519.4A patent/CN106106146B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN106035089B (en) | 2018-05-15 |
| CN105918135A (en) | 2016-09-07 |
| CN105918135B (en) | 2018-06-01 |
| CN106106146A (en) | 2016-11-16 |
| CN105993957B (en) | 2017-12-08 |
| CN106035089A (en) | 2016-10-26 |
| CN104855291A (en) | 2015-08-26 |
| CN105993957A (en) | 2016-10-12 |
| CN106106146B (en) | 2018-05-08 |
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