Tissue culture system and its LED light incubator
The application is
Application No.:201510299391.6
The applying date is:On June 4th, 2015
Apply entitled:Tissue culture system
Patent application divisional application.
Technical field
The present invention relates to a kind of tissue culture system for plants such as dendrobium candidums.
Background technology
Dendrobium candidum, it is one of kind the most precious in China's orchid family Dendrobium Sw.Because there is enhancing body to exempt from for it
Epidemic disease power, antitumor, anti-aging and the effectively effect such as mitigation hepatic injury, in version in 2010《Pharmacopoeia of People's Republic of China》Middle coverlet
Medicinal material simply solely is classified as, due to artificial excessively excavation and the destruction of ecological environment, its own low reproduction rate in addition, wild iron sheet stone
Dry measure used in former times resource is increasingly exhausted, is classified as the rare or endangered species of two level focused protection by country.Now, artificial cultivation plantation iron sheet stone
Dry measure used in former times turns into the main path for meeting great market demand.Dendrobium candidum system orchid family Dendrobium Sw, seed is minimum, without endosperm,
Need to sprout with some mycosymbiosises under natural conditions, germination percentage only a ten thousandth in the case of nature, and cultivar is present
Degradation phenomena.
In recent years, some scientific research institutions in China and business unit are carried out to the tissue culture and artificial cultivation technique of dendrobium candidum
Substantial amounts of exploration and experiment, achieve some achievements, also make it that artificial cultivation dendrobium candidum yield increased in recent years.But
It is that the artificial culture of dendrobium candidum still has that seeding propagation speed is slow, training tissue culture seedling and transplanting survival rate are low, artificial growth
The problem of product active constituent content is low, seriously constrain industrialization, the large-scale development of dendrobium candidum.In addition, dendrobium candidum
General field planting can be with growth of weeds, and be vulnerable to pest and disease damage.
The light that Publication No. CN102057867A patent document discloses a kind of tissue cultures of seedlings of Dendrobium officinale is adjusted
Prosecutor method, including powdered seed is obtained after being sterilized by Dendrobium officinale fruit, powdered seed is trained by seed germination medium
Support, sprout the process for forming protocorm, it is characterised in that in the tissue culture procedures of seedlings of Dendrobium officinale, (semiconductor is sent out with LED
Optical diode) plant growth lighting system as light source, according to the different phase of dendrobium candidum tissue culture, implements different light
According to processing.Implement different photo-irradiation treatments by using different growth phases, seedlings of Dendrobium officinale is maximally utilised light
Its photosynthesis can be supplied, so as to obtain maximum growth amount.
In the tissue culture system of prior art, light quality ratio, the intensity of illumination of red blue mixed light can not be conveniently and efficiently adjusted
And periodicity of illumination.
The content of the invention
In order to solve above-mentioned technical problem, it is an object of the invention to provide a kind of tissue culture system, LED optical culture is inside set
Case, light quality ratio, intensity of illumination and the illumination week of red blue mixed light can be conveniently adjusted using planar emission apparatus
Phase.
To achieve the above object, present invention employs following technical scheme:
A kind of tissue culture system, including totally enclosed tissue culture transfer room and artificial training seedling room, described tissue culture transfer room and people
Work training seedling room or so is disposed adjacent, and the entrance of tissue culture transfer room and artificial training seedling room is connected with the outlet of Airshower chamber, described group
Train and multiple vaccination stations and a conveyer belt are set in transfer room, the output end of conveyer belt is stretched into artificial training seedling room, described artificial
Train and training seedling interior room is provided with seedling room, it is artificial to train between seedling room and training seedling interior room only by several ventilation window ventilating airs, from
It is artificial to train seedling room by a sliding door into training seedling interior room, train indoor provided with constant-temperature constant-humidity air-conditioner and the training of multiple LED lamplights in seedling
Support case;Several ventilation fans with ft connection, the artificial outside peace for training seedling room are installed on the wall of the artificial training seedling room
Equipped with steel framework, sunshade net is laid at the top of steel framework, sunshade net is furled and deployed by drawstring, is provided with steel framework
For the pulley of drawstring traction;The LED light incubator includes casing, sets tissue culture bottle conveying device and more in casing respectively
Individual planar emission apparatus;Tissue culture bottle conveying device includes multiple chains, and each chain is arranged on by sprocket wheel in casing and head and the tail
Connection forms the motion cycle in vertical plane, and the motion cycle of multiple chains is parallel to each other and is driven by same drive device same
Step is moved, and sets multiple loop brackets that can place tissue culture bottle between adjacent chain, so, multiple chains are just formed vertical
The structure for conveying of circulation conveying tissue culture bottle in face, loop bracket include filler ring and the connecting shaft being fixedly connected, group are put into filler ring
Bottle is trained, tissue culture bottle is fixed in filler ring by the flange bearing on bottle or bottle cap circumference, the connecting shaft and chain of loop bracket
Link units connection, the link units include outer plate, inner plate, roller, sleeve and self lubrication bearing, and two inner plates lead to
Cross sleeve and be fixedly connected to form inner link, bush outer sleeve sets roller, and self lubrication bearing is plugged in sleeve, and self lubrication bearing passes set
The both ends of cylinder are arranged two outer plates, connecting shaft are plugged in self lubrication bearing, the both ends that connecting shaft passes self lubrication bearing cover
Provided with plane bearing and self-locking nut, self-locking nut is threadedly coupled with connecting shaft and plane bearing is pressed on the outside of outer plate;It is flat
Surface illuminating device includes light guide plate, LED aluminum base plate and radiating wing plate, and LED aluminum base plate front is provided with a manner of array distribution
Multiple red LED lamps and multiple blue LED lamps, the incidence surface of light guide plate press close to the front of aluminium base, and radiating wing plate is close to aluminium
The back side of substrate;In the conveying circulation that multiple chains that one planar emission apparatus is arranged on tissue culture bottle conveying device are formed
Portion, the conveying circulation outside that multiple chains that another planar emission apparatus is arranged on tissue culture bottle conveying device are formed, this two
Individual planar emission apparatus is defeated to this inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed respectively
A vertical plane of circulation is sent to carry out illumination.
Preferably, constant-temperature constant-humidity air-conditioner is installed in the tissue culture transfer room.
Preferably, the vaccination station includes cabinet, the front and rear sides of cabinet are transparent plastic sheet, the left and right sides of cabinet
Quartz burner is installed, cabinet inside is provided with temperature sensor, humidity sensor and welding rifle, cabinet inwall top on inwall
Provided with air blowing hole array, cabinet inwall bottom is provided with ventilation hole array.
Preferably, the tissue culture transfer room, artificial training seedling room and training seedling interior room are all white by aluminum alloy frame and float glass process
Glass is built.
Preferably, the tissue culture bottle includes the bottle and bottle cap of threaded connection, bottle cap center is provided with central tube, works as bottle
When inside having culture medium, sterile cotton is filled in central tube and by closing of sealing with wax.
Preferably, to the conveying inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed
Two planar emission apparatus that one vertical plane of circulation carries out illumination are separately fixed in two mobile bases, the two movements
Base is slidably connected with casing by track level, and bottom half sets up a screw mandrel, and screw mandrel passes through plus thread and left-hand thread respectively
Two feed screw nuts are connected with, two feed screw nuts are fixedly connected with two mobile bases respectively, a motor or runner driving silk
Bar rotates, so as to drive the conveying circulation that two planar emission apparatus are formed from multiple chains of tissue culture bottle conveying device respectively
Inside and outside synchronously closer or far from the conveying circulation the vertical plane by illumination.
Preferably, the red LED lamp and blue LED lamp in the LED aluminum base plate distribute alternately, controller control is each
Red LED lamp and blue LED lamp are individually lighted or extinguished.Preferably, the power of the red LED lamp and blue LED lamp is equal
For 1W, the red spectral band of red LED lamp is 450 ± 20nm, and the blue wave band of blue LED lamp is 660 ± 20nm.Preferably,
The mobile base is provided with the wind that can promote the circulation circulation of casing interior air-flow in the radiating wing plate side frames of planar emission apparatus
Fan, the ventilation fan that can carry out that air-flow exchanges inside and outside casing is installed on the casing.
Technical scheme of the present invention as a result of more than, inside sets LED light incubator, can using planar emission apparatus
Conveniently adjust light quality ratio, intensity of illumination and the periodicity of illumination of red blue mixed light.Connect by Airshower chamber into tissue culture
Kind room and artificial training seedling room ensure that the cleanliness factor of tissue culture room, by setting manually training seedling room and training seedling interior room to ensure that tissue culture room
Interior temperature and humidity, sunshade net and LED light incubator are set, dendrobium candidum group ensure that by natural light and artificial light
Training seedling germination, growth and the illumination of hardening needs, and so, can not only greatly improve the survival rate of dendrobium candidum, and save
The time cultivated, cultivation cost is reduced, improve economic benefit.
Brief description of the drawings
Fig. 1 is the structural representation of tissue culture system of the present invention;
Fig. 2 is the structural representation of LED light incubator;
Fig. 3 is the partial structural diagram of more chain conveying structures;
Fig. 4 is the cooperation schematic diagram of tissue culture bottle, loop bracket and link unit;
Fig. 5 is the moving structure schematic diagram of planar emission apparatus;
Fig. 6 is the aeration structure schematic diagram of planar emission apparatus.
Embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is shown in the drawings, wherein from beginning to end
Same or similar label represents same or similar element or the element with same or like function.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to for explaining the present invention, and is not considered as limiting the invention.
In the description of the invention, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", " on ", " under ", "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom " " interior ", " clockwise ", " inverse
The orientation or position relationship of the instructions such as hour hands " are based on orientation shown in the drawings or position relationship, are for only for ease of description originally
Invent and simplify description, rather than the device or element of instruction or hint meaning there must be specific orientation, with specific side
Position construction and operation, therefore be not considered as limiting the invention.
Tissue culture system as shown in Figure 1, including totally enclosed tissue culture transfer room 2 and artificial training seedling room 3, described tissue culture connect
Kind of room 2 and artificial training seedling room 3 or so are disposed adjacent, and tissue culture transfer room 2 and are manually trained the entrance of seedling room 3 and are gone out with Airshower chamber 1
Mouth connection, the tissue culture transfer room 2 is interior to set multiple vaccination stations 21 and a conveyer belt 22, the output end of conveyer belt 22 to stretch into people
In work training seedling room 3, training seedling interior room 4 is provided with the artificial training seedling room 3, if manually between training seedling room 3 and training seedling interior room 4 only by
Dry ventilation window ventilating air, enter training seedling interior room 4 from artificial training seedling room 3 by a sliding door, perseverance is provided with training seedling interior room 4
Constant temperature and humidity air-conditioning and multiple LED light incubators 41;It is provided with the wall of the artificial training seedling room 3 some with ft connection
Individual ventilation fan 31.The outside of artificial training seedling room 3 is provided with steel framework, lays sunshade net at the top of steel framework, sunshade net passes through
Drawstring furls and expansion, and the pulley for drawstring traction is provided with steel framework.Constant temperature is installed in the tissue culture transfer room 2
Constant wetting air conditioner.The vaccination station 21 includes cabinet, and the front and rear sides of cabinet are transparent plastic sheet, on the left and right sides inwall of cabinet
Quartz burner is installed, cabinet inside is provided with temperature sensor, humidity sensor and welding rifle, is provided with and blows at the top of cabinet inwall
Stomata array, cabinet inwall bottom are provided with ventilation hole array.The tissue culture transfer room 2, artificial training seedling room 3 and training seedling interior room 4 are all
It is to be built by aluminum alloy frame and float glass process white glass.Tissue culture transfer room is entered by Airshower chamber and artificial training seedling room ensure that
The cleanliness factor of tissue culture room, the temperature and humidity in tissue culture room is ensure that by the artificial training seedling room of setting and training seedling interior room, and setting hides
Screened postive and LED light incubator, candidum tissue culturing seedling germination, growth and hardening ensure that by natural light and artificial light
Illumination needs, and so, can not only greatly improve the survival rate of dendrobium candidum, and has saved the time cultivated, and reduces training
This is bred as, improves economic benefit.
As shown in Fig. 2 the LED light incubator includes casing 411, tissue culture bottle conveying dress is set respectively in casing 411
Put and multiple planar emission apparatus 412;Tissue culture bottle conveying device includes multiple chains 414, and each chain 414 is set by sprocket wheel
In casing 411 and head and the tail connect the motion cycle formed in vertical plane, and the motion cycle of multiple chains 414 is parallel to each other simultaneously
It is synchronized with the movement by the driving of same drive device, multiple loop brackets that can place tissue culture bottle is set between adjacent chain 414
415, so, multiple chains 414 just form the structure for conveying of the circulation conveying tissue culture bottle in vertical plane, as shown in Figure 3, Figure 4, ring
Shape bracket 415 includes filler ring 4151 and the connecting shaft 4152 being fixedly connected, and is put into tissue culture bottle in filler ring 4151, tissue culture bottle passes through bottle
The supporting and fixing of flange 622 on body 61 or the circumference of bottle cap 62 is in filler ring 4151, the connecting shaft 4152 and chain of loop bracket 415
414 link units connection, the link units include outer plate 4141, inner plate 4142, roller 4143, sleeve 4145 and Zi Run
Slide-bar set 4147, two inner plates 4142 are fixedly connected to form inner link by sleeve 4145, roller are arranged outside sleeve 4145
4143, plug self lubrication bearing 4147 in sleeve 4145, self lubrication bearing 4147 pass sleeve 4145 both ends be arranged two it is outer
Carrier bar 4141, connecting shaft 4152 is plugged in self lubrication bearing 4147, the both ends that connecting shaft 4152 passes self lubrication bearing 4147 are equal
It is arranged with plane bearing 4144 and self-locking nut 4146, self-locking nut 4146 is threadedly coupled with connecting shaft 4152 and by plane bearing
4144 are pressed in the outside of outer plate 4141;As shown in figure 5, planar emission apparatus 412 includes light guide plate 4121, LED aluminum base plate 4122
With radiating wing plate 4123, the front of LED aluminum base plate 4122 is provided with multiple red LED lamps and multiple blue lights in a manner of array distribution
LED, the incidence surface of light guide plate 4121 press close to the front of aluminium base 4122, and radiating wing plate 4123 is close to the back of the body of aluminium base 4122
Face, projected after using light guide plate that the light that its bottom LED point light source is sent can be carried out to Mixed adjustment in the form of area source,
Uniform illumination and reddish blue light mixes, cost is low;As shown in Fig. 2 to be arranged on tissue culture bottle defeated for a planar emission apparatus 412
The conveying circulation for sending multiple chains of device to be formed is internal, and another planar emission apparatus 412 is arranged on tissue culture bottle conveying dress
The conveying circulation outside that the multiple chains put are formed, the two planar emission apparatus 412 are respectively from tissue culture bottle conveying device
Illumination is carried out to a vertical plane of conveying circulation inside and outside the conveying circulation that multiple chains are formed.
In the present embodiment, as shown in figure 4, the tissue culture bottle includes the bottle 61 and bottle cap 62 of threaded connection, in bottle cap 62
Centre is provided with central tube 621, when having culture medium in bottle 61, sterile cotton 64 is filled in central tube 621 and by 63 closings of sealing with wax.
As shown in figure 5, to conveying circulation inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed
Two planar emission apparatus 412 that one vertical plane carries out illumination are separately fixed in two mobile bases 413, the two movements
Base 413 is slidably connected with casing 411 by track level, and the bottom of casing 411 sets up a screw mandrel 416, and screw mandrel 416 passes through respectively
Plus thread and left-hand thread are connected with two feed screw nuts 417, and two feed screw nuts 417 fix with two mobile bases 413 respectively
Connection, a motor 418 or runner drive screw 416 rotate, so as to drive two planar emission apparatus 412 defeated from tissue culture bottle respectively
Send inside and outside the conveying circulation that multiple chains of device are formed synchronously closer or far from conveying circulation by illumination
Vertical plane.Spacing so by adjusting two planar emission apparatus, it becomes possible to adjust light of the planar emission apparatus to tissue culture face
According to intensity, equipment applicability is stronger, and cost is low.Motor control is such as used, and corresponding light sensor is set just in casing
Automatically controlling for intensity of illumination can be realized.The power of the red LED lamp and blue LED lamp is 1W, red LED lamp it is red
Optical band is 450 ± 20nm, and the blue wave band of blue LED lamp is 660 ± 20nm.Red-light LED in the LED aluminum base plate 4122
Lamp and blue LED lamp are distributed alternately with spacing 2cm × 2cm, and controller controls each red LED lamp and the independent point of blue LED lamp
It is bright or extinguish, so by controlling lighting quantity and distribution and just can easily adjusting plane for red LED lamp and blue LED lamp
The light quality ratio of light-emitting device.The mobile base 413 is provided with energy in the side frames of radiating wing plate 4123 of planar emission apparatus 412
Enough promote the fan 419 of the interior air-flow of casing 411 circulation circulation, outer gas in casing 411 can be carried out by being provided with the casing 411
Flow the ventilation fan 420 exchanged.So, LED can not only be helped to radiate, and LED can be maintained using these heats
Temperature in light incubator, when temperature is higher in LED light incubator, it can also radiate to training seedling interior room etc..
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art is not departing from the principle and objective of the present invention
In the case of above-described embodiment can be changed within the scope of the invention, change, replace and modification.