CN105993957B - Tissue culture system and its led light incubator - Google Patents

Tissue culture system and its led light incubator Download PDF

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Publication number
CN105993957B
CN105993957B CN201610472548.5A CN201610472548A CN105993957B CN 105993957 B CN105993957 B CN 105993957B CN 201610472548 A CN201610472548 A CN 201610472548A CN 105993957 B CN105993957 B CN 105993957B
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China
Prior art keywords
tissue culture
conveying
bottle
circulation
culture bottle
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CN201610472548.5A
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Chinese (zh)
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CN105993957A (en
Inventor
孙建华
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Shaoxing Diyue door industry limited company
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Shaoxing Diyue Door Industry Ltd Co
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The invention discloses a kind of tissue culture system and its LED light incubator, the LED light incubator includes casing, and tissue culture bottle conveying device and multiple planar emission apparatus are set respectively in casing;Tissue culture bottle conveying device includes multiple chains, the motion cycle of multiple chains is parallel to each other and is synchronized with the movement by the driving of same drive device, multiple loop brackets that can place tissue culture bottle are set between adjacent chain, loop bracket includes filler ring and the connecting shaft being fixedly connected, tissue culture bottle is put into filler ring, it is internal that one planar emission apparatus is arranged on the conveying circulation that multiple chains are formed, another planar emission apparatus is arranged on the conveying circulation outside that multiple chains are formed, and the two planar emission apparatus carry out illumination from the vertical plane circulated to the conveying respectively.This programme can conveniently adjust light quality ratio, intensity of illumination and the periodicity of illumination of red blue mixed light.

Description

Tissue culture system and its LED light incubator
The application is
Application No.:201510299391.6
The applying date is:On June 4th, 2015
Apply entitled:Tissue culture system
Patent application divisional application.
Technical field
The present invention relates to a kind of tissue culture system for plants such as dendrobium candidums.
Background technology
Dendrobium candidum, it is one of kind the most precious in China's orchid family Dendrobium Sw.Because there is enhancing body to exempt from for it Epidemic disease power, antitumor, anti-aging and the effectively effect such as mitigation hepatic injury, in version in 2010《Pharmacopoeia of People's Republic of China》Middle coverlet Medicinal material simply solely is classified as, due to artificial excessively excavation and the destruction of ecological environment, its own low reproduction rate in addition, wild iron sheet stone Dry measure used in former times resource is increasingly exhausted, is classified as the rare or endangered species of two level focused protection by country.Now, artificial cultivation plantation iron sheet stone Dry measure used in former times turns into the main path for meeting great market demand.Dendrobium candidum system orchid family Dendrobium Sw, seed is minimum, without endosperm, Need to sprout with some mycosymbiosises under natural conditions, germination percentage only a ten thousandth in the case of nature, and cultivar is present Degradation phenomena.
In recent years, some scientific research institutions in China and business unit are carried out to the tissue culture and artificial cultivation technique of dendrobium candidum Substantial amounts of exploration and experiment, achieve some achievements, also make it that artificial cultivation dendrobium candidum yield increased in recent years.But It is that the artificial culture of dendrobium candidum still has that seeding propagation speed is slow, training tissue culture seedling and transplanting survival rate are low, artificial growth The problem of product active constituent content is low, seriously constrain industrialization, the large-scale development of dendrobium candidum.In addition, dendrobium candidum General field planting can be with growth of weeds, and be vulnerable to pest and disease damage.
The light that Publication No. CN102057867A patent document discloses a kind of tissue cultures of seedlings of Dendrobium officinale is adjusted Prosecutor method, including powdered seed is obtained after being sterilized by Dendrobium officinale fruit, powdered seed is trained by seed germination medium Support, sprout the process for forming protocorm, it is characterised in that in the tissue culture procedures of seedlings of Dendrobium officinale, (semiconductor is sent out with LED Optical diode) plant growth lighting system as light source, according to the different phase of dendrobium candidum tissue culture, implements different light According to processing.Implement different photo-irradiation treatments by using different growth phases, seedlings of Dendrobium officinale is maximally utilised light Its photosynthesis can be supplied, so as to obtain maximum growth amount.
In the tissue culture system of prior art, light quality ratio, the intensity of illumination of red blue mixed light can not be conveniently and efficiently adjusted And periodicity of illumination.
The content of the invention
In order to solve above-mentioned technical problem, it is an object of the invention to provide a kind of tissue culture system, LED optical culture is inside set Case, light quality ratio, intensity of illumination and the illumination week of red blue mixed light can be conveniently adjusted using planar emission apparatus Phase.
To achieve the above object, present invention employs following technical scheme:
A kind of tissue culture system, including totally enclosed tissue culture transfer room and artificial training seedling room, described tissue culture transfer room and people Work training seedling room or so is disposed adjacent, and the entrance of tissue culture transfer room and artificial training seedling room is connected with the outlet of Airshower chamber, described group Train and multiple vaccination stations and a conveyer belt are set in transfer room, the output end of conveyer belt is stretched into artificial training seedling room, described artificial Train and training seedling interior room is provided with seedling room, it is artificial to train between seedling room and training seedling interior room only by several ventilation window ventilating airs, from It is artificial to train seedling room by a sliding door into training seedling interior room, train indoor provided with constant-temperature constant-humidity air-conditioner and the training of multiple LED lamplights in seedling Support case;Several ventilation fans with ft connection, the artificial outside peace for training seedling room are installed on the wall of the artificial training seedling room Equipped with steel framework, sunshade net is laid at the top of steel framework, sunshade net is furled and deployed by drawstring, is provided with steel framework For the pulley of drawstring traction;The LED light incubator includes casing, sets tissue culture bottle conveying device and more in casing respectively Individual planar emission apparatus;Tissue culture bottle conveying device includes multiple chains, and each chain is arranged on by sprocket wheel in casing and head and the tail Connection forms the motion cycle in vertical plane, and the motion cycle of multiple chains is parallel to each other and is driven by same drive device same Step is moved, and sets multiple loop brackets that can place tissue culture bottle between adjacent chain, so, multiple chains are just formed vertical The structure for conveying of circulation conveying tissue culture bottle in face, loop bracket include filler ring and the connecting shaft being fixedly connected, group are put into filler ring Bottle is trained, tissue culture bottle is fixed in filler ring by the flange bearing on bottle or bottle cap circumference, the connecting shaft and chain of loop bracket Link units connection, the link units include outer plate, inner plate, roller, sleeve and self lubrication bearing, and two inner plates lead to Cross sleeve and be fixedly connected to form inner link, bush outer sleeve sets roller, and self lubrication bearing is plugged in sleeve, and self lubrication bearing passes set The both ends of cylinder are arranged two outer plates, connecting shaft are plugged in self lubrication bearing, the both ends that connecting shaft passes self lubrication bearing cover Provided with plane bearing and self-locking nut, self-locking nut is threadedly coupled with connecting shaft and plane bearing is pressed on the outside of outer plate;It is flat Surface illuminating device includes light guide plate, LED aluminum base plate and radiating wing plate, and LED aluminum base plate front is provided with a manner of array distribution Multiple red LED lamps and multiple blue LED lamps, the incidence surface of light guide plate press close to the front of aluminium base, and radiating wing plate is close to aluminium The back side of substrate;In the conveying circulation that multiple chains that one planar emission apparatus is arranged on tissue culture bottle conveying device are formed Portion, the conveying circulation outside that multiple chains that another planar emission apparatus is arranged on tissue culture bottle conveying device are formed, this two Individual planar emission apparatus is defeated to this inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed respectively A vertical plane of circulation is sent to carry out illumination.
Preferably, constant-temperature constant-humidity air-conditioner is installed in the tissue culture transfer room.
Preferably, the vaccination station includes cabinet, the front and rear sides of cabinet are transparent plastic sheet, the left and right sides of cabinet Quartz burner is installed, cabinet inside is provided with temperature sensor, humidity sensor and welding rifle, cabinet inwall top on inwall Provided with air blowing hole array, cabinet inwall bottom is provided with ventilation hole array.
Preferably, the tissue culture transfer room, artificial training seedling room and training seedling interior room are all white by aluminum alloy frame and float glass process Glass is built.
Preferably, the tissue culture bottle includes the bottle and bottle cap of threaded connection, bottle cap center is provided with central tube, works as bottle When inside having culture medium, sterile cotton is filled in central tube and by closing of sealing with wax.
Preferably, to the conveying inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed Two planar emission apparatus that one vertical plane of circulation carries out illumination are separately fixed in two mobile bases, the two movements Base is slidably connected with casing by track level, and bottom half sets up a screw mandrel, and screw mandrel passes through plus thread and left-hand thread respectively Two feed screw nuts are connected with, two feed screw nuts are fixedly connected with two mobile bases respectively, a motor or runner driving silk Bar rotates, so as to drive the conveying circulation that two planar emission apparatus are formed from multiple chains of tissue culture bottle conveying device respectively Inside and outside synchronously closer or far from the conveying circulation the vertical plane by illumination.
Preferably, the red LED lamp and blue LED lamp in the LED aluminum base plate distribute alternately, controller control is each Red LED lamp and blue LED lamp are individually lighted or extinguished.Preferably, the power of the red LED lamp and blue LED lamp is equal For 1W, the red spectral band of red LED lamp is 450 ± 20nm, and the blue wave band of blue LED lamp is 660 ± 20nm.Preferably, The mobile base is provided with the wind that can promote the circulation circulation of casing interior air-flow in the radiating wing plate side frames of planar emission apparatus Fan, the ventilation fan that can carry out that air-flow exchanges inside and outside casing is installed on the casing.
Technical scheme of the present invention as a result of more than, inside sets LED light incubator, can using planar emission apparatus Conveniently adjust light quality ratio, intensity of illumination and the periodicity of illumination of red blue mixed light.Connect by Airshower chamber into tissue culture Kind room and artificial training seedling room ensure that the cleanliness factor of tissue culture room, by setting manually training seedling room and training seedling interior room to ensure that tissue culture room Interior temperature and humidity, sunshade net and LED light incubator are set, dendrobium candidum group ensure that by natural light and artificial light Training seedling germination, growth and the illumination of hardening needs, and so, can not only greatly improve the survival rate of dendrobium candidum, and save The time cultivated, cultivation cost is reduced, improve economic benefit.
Brief description of the drawings
Fig. 1 is the structural representation of tissue culture system of the present invention;
Fig. 2 is the structural representation of LED light incubator;
Fig. 3 is the partial structural diagram of more chain conveying structures;
Fig. 4 is the cooperation schematic diagram of tissue culture bottle, loop bracket and link unit;
Fig. 5 is the moving structure schematic diagram of planar emission apparatus;
Fig. 6 is the aeration structure schematic diagram of planar emission apparatus.
Embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is shown in the drawings, wherein from beginning to end Same or similar label represents same or similar element or the element with same or like function.Below with reference to attached The embodiment of figure description is exemplary, it is intended to for explaining the present invention, and is not considered as limiting the invention.
In the description of the invention, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " length ", " width ", " thickness ", " on ", " under ", "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom " " interior ", " clockwise ", " inverse The orientation or position relationship of the instructions such as hour hands " are based on orientation shown in the drawings or position relationship, are for only for ease of description originally Invent and simplify description, rather than the device or element of instruction or hint meaning there must be specific orientation, with specific side Position construction and operation, therefore be not considered as limiting the invention.
Tissue culture system as shown in Figure 1, including totally enclosed tissue culture transfer room 2 and artificial training seedling room 3, described tissue culture connect Kind of room 2 and artificial training seedling room 3 or so are disposed adjacent, and tissue culture transfer room 2 and are manually trained the entrance of seedling room 3 and are gone out with Airshower chamber 1 Mouth connection, the tissue culture transfer room 2 is interior to set multiple vaccination stations 21 and a conveyer belt 22, the output end of conveyer belt 22 to stretch into people In work training seedling room 3, training seedling interior room 4 is provided with the artificial training seedling room 3, if manually between training seedling room 3 and training seedling interior room 4 only by Dry ventilation window ventilating air, enter training seedling interior room 4 from artificial training seedling room 3 by a sliding door, perseverance is provided with training seedling interior room 4 Constant temperature and humidity air-conditioning and multiple LED light incubators 41;It is provided with the wall of the artificial training seedling room 3 some with ft connection Individual ventilation fan 31.The outside of artificial training seedling room 3 is provided with steel framework, lays sunshade net at the top of steel framework, sunshade net passes through Drawstring furls and expansion, and the pulley for drawstring traction is provided with steel framework.Constant temperature is installed in the tissue culture transfer room 2 Constant wetting air conditioner.The vaccination station 21 includes cabinet, and the front and rear sides of cabinet are transparent plastic sheet, on the left and right sides inwall of cabinet Quartz burner is installed, cabinet inside is provided with temperature sensor, humidity sensor and welding rifle, is provided with and blows at the top of cabinet inwall Stomata array, cabinet inwall bottom are provided with ventilation hole array.The tissue culture transfer room 2, artificial training seedling room 3 and training seedling interior room 4 are all It is to be built by aluminum alloy frame and float glass process white glass.Tissue culture transfer room is entered by Airshower chamber and artificial training seedling room ensure that The cleanliness factor of tissue culture room, the temperature and humidity in tissue culture room is ensure that by the artificial training seedling room of setting and training seedling interior room, and setting hides Screened postive and LED light incubator, candidum tissue culturing seedling germination, growth and hardening ensure that by natural light and artificial light Illumination needs, and so, can not only greatly improve the survival rate of dendrobium candidum, and has saved the time cultivated, and reduces training This is bred as, improves economic benefit.
As shown in Fig. 2 the LED light incubator includes casing 411, tissue culture bottle conveying dress is set respectively in casing 411 Put and multiple planar emission apparatus 412;Tissue culture bottle conveying device includes multiple chains 414, and each chain 414 is set by sprocket wheel In casing 411 and head and the tail connect the motion cycle formed in vertical plane, and the motion cycle of multiple chains 414 is parallel to each other simultaneously It is synchronized with the movement by the driving of same drive device, multiple loop brackets that can place tissue culture bottle is set between adjacent chain 414 415, so, multiple chains 414 just form the structure for conveying of the circulation conveying tissue culture bottle in vertical plane, as shown in Figure 3, Figure 4, ring Shape bracket 415 includes filler ring 4151 and the connecting shaft 4152 being fixedly connected, and is put into tissue culture bottle in filler ring 4151, tissue culture bottle passes through bottle The supporting and fixing of flange 622 on body 61 or the circumference of bottle cap 62 is in filler ring 4151, the connecting shaft 4152 and chain of loop bracket 415 414 link units connection, the link units include outer plate 4141, inner plate 4142, roller 4143, sleeve 4145 and Zi Run Slide-bar set 4147, two inner plates 4142 are fixedly connected to form inner link by sleeve 4145, roller are arranged outside sleeve 4145 4143, plug self lubrication bearing 4147 in sleeve 4145, self lubrication bearing 4147 pass sleeve 4145 both ends be arranged two it is outer Carrier bar 4141, connecting shaft 4152 is plugged in self lubrication bearing 4147, the both ends that connecting shaft 4152 passes self lubrication bearing 4147 are equal It is arranged with plane bearing 4144 and self-locking nut 4146, self-locking nut 4146 is threadedly coupled with connecting shaft 4152 and by plane bearing 4144 are pressed in the outside of outer plate 4141;As shown in figure 5, planar emission apparatus 412 includes light guide plate 4121, LED aluminum base plate 4122 With radiating wing plate 4123, the front of LED aluminum base plate 4122 is provided with multiple red LED lamps and multiple blue lights in a manner of array distribution LED, the incidence surface of light guide plate 4121 press close to the front of aluminium base 4122, and radiating wing plate 4123 is close to the back of the body of aluminium base 4122 Face, projected after using light guide plate that the light that its bottom LED point light source is sent can be carried out to Mixed adjustment in the form of area source, Uniform illumination and reddish blue light mixes, cost is low;As shown in Fig. 2 to be arranged on tissue culture bottle defeated for a planar emission apparatus 412 The conveying circulation for sending multiple chains of device to be formed is internal, and another planar emission apparatus 412 is arranged on tissue culture bottle conveying dress The conveying circulation outside that the multiple chains put are formed, the two planar emission apparatus 412 are respectively from tissue culture bottle conveying device Illumination is carried out to a vertical plane of conveying circulation inside and outside the conveying circulation that multiple chains are formed.
In the present embodiment, as shown in figure 4, the tissue culture bottle includes the bottle 61 and bottle cap 62 of threaded connection, in bottle cap 62 Centre is provided with central tube 621, when having culture medium in bottle 61, sterile cotton 64 is filled in central tube 621 and by 63 closings of sealing with wax. As shown in figure 5, to conveying circulation inside and outside the conveying circulation that multiple chains of tissue culture bottle conveying device are formed Two planar emission apparatus 412 that one vertical plane carries out illumination are separately fixed in two mobile bases 413, the two movements Base 413 is slidably connected with casing 411 by track level, and the bottom of casing 411 sets up a screw mandrel 416, and screw mandrel 416 passes through respectively Plus thread and left-hand thread are connected with two feed screw nuts 417, and two feed screw nuts 417 fix with two mobile bases 413 respectively Connection, a motor 418 or runner drive screw 416 rotate, so as to drive two planar emission apparatus 412 defeated from tissue culture bottle respectively Send inside and outside the conveying circulation that multiple chains of device are formed synchronously closer or far from conveying circulation by illumination Vertical plane.Spacing so by adjusting two planar emission apparatus, it becomes possible to adjust light of the planar emission apparatus to tissue culture face According to intensity, equipment applicability is stronger, and cost is low.Motor control is such as used, and corresponding light sensor is set just in casing Automatically controlling for intensity of illumination can be realized.The power of the red LED lamp and blue LED lamp is 1W, red LED lamp it is red Optical band is 450 ± 20nm, and the blue wave band of blue LED lamp is 660 ± 20nm.Red-light LED in the LED aluminum base plate 4122 Lamp and blue LED lamp are distributed alternately with spacing 2cm × 2cm, and controller controls each red LED lamp and the independent point of blue LED lamp It is bright or extinguish, so by controlling lighting quantity and distribution and just can easily adjusting plane for red LED lamp and blue LED lamp The light quality ratio of light-emitting device.The mobile base 413 is provided with energy in the side frames of radiating wing plate 4123 of planar emission apparatus 412 Enough promote the fan 419 of the interior air-flow of casing 411 circulation circulation, outer gas in casing 411 can be carried out by being provided with the casing 411 Flow the ventilation fan 420 exchanged.So, LED can not only be helped to radiate, and LED can be maintained using these heats Temperature in light incubator, when temperature is higher in LED light incubator, it can also radiate to training seedling interior room etc..
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art is not departing from the principle and objective of the present invention In the case of above-described embodiment can be changed within the scope of the invention, change, replace and modification.

Claims (4)

1. a kind of LED light incubator, including casing (411), it is characterised in that tissue culture is set respectively in the casing (411) Bottle conveying device and multiple planar emission apparatus (412);Tissue culture bottle conveying device includes multiple chains (414), each chain (414) it is arranged on by sprocket wheel in casing (411) and head and the tail connects the motion cycle formed in vertical plane, multiple chains (414) motion cycle is parallel to each other and is synchronized with the movement by the driving of same drive device, is set between adjacent chain (414) multiple The loop bracket (415) of tissue culture bottle can be placed, so, multiple chains (414) just form the circulation conveying tissue culture in vertical plane The structure for conveying of bottle, loop bracket (415) include filler ring (4151) and the connecting shaft (4152) being fixedly connected, and filler ring (4151) is interior Tissue culture bottle is put into, tissue culture bottle is by flange (622) supporting and fixing on bottle (61) or bottle cap (62) circumference in filler ring (4151) Interior, the connecting shaft (4152) of loop bracket (415) is connected with the link units of chain (414), and the link units include outer plate (4141), inner plate (4142), roller (4143), sleeve (4145) and self lubrication bearing (4147), two inner plates (4142) Inner link is fixedly connected to form by sleeve (4145), roller (4143) is arranged outside sleeve (4145), is plugged in sleeve (4145) Self lubrication bearing (4147), the both ends that self lubrication bearing (4147) passes sleeve (4145) are arranged two outer plates (4141), from Connecting shaft (4152) is plugged in lubricative axle sleeve (4147), the both ends that connecting shaft (4152) passes self lubrication bearing (4147) are arranged There are a plane bearing (4144) and self-locking nut (4146), self-locking nut (4146) is threadedly coupled with connecting shaft (4152) and by plane Bearing (4144) is pressed on the outside of outer plate (4141);Planar emission apparatus (412) includes light guide plate (4121), LED aluminum base plate (4122) and radiating wing plate (4123), LED aluminum base plate (4122) front are provided with multiple red LED lamps in a manner of array distribution With multiple blue LED lamps, the incidence surface of light guide plate (4121) presses close to the front of aluminium base (4122), and radiating wing plate (4123) is close to At the back side of aluminium base (4122);One planar emission apparatus (412) is arranged on multiple chain institutes shape of tissue culture bottle conveying device Into conveying circulate internal, multiple chains that another planar emission apparatus (412) is arranged on tissue culture bottle conveying device are formed Conveying circulation outside, what the two planar emission apparatus (412) were formed from multiple chains of tissue culture bottle conveying device respectively Illumination is carried out to a vertical plane of conveying circulation inside and outside conveying circulation;The tissue culture bottle includes the bottle of threaded connection Body (61) and bottle cap (62), bottle cap (62) center are provided with central tube (621), when having culture medium in bottle (61), central tube (621) sterile cotton (64) is filled in and by (63) closing of sealing with wax;Formed from multiple chains of tissue culture bottle conveying device defeated Send two planar emission apparatus (412) of the vertical plane progress illumination inside and outside circulation to conveying circulation solid respectively It is scheduled on two mobile bases (413), the two mobile bases (413) are slidably connected with casing (411) by track level, case Body (411) bottom sets up a screw mandrel (416), and screw mandrel (416) is connected with two feed screw nuts by plus thread and left-hand thread respectively (417), two feed screw nuts (417) are fixedly connected with two mobile bases (413) respectively, a motor (418) or runner driving Screw mandrel (416) rotates, so as to drive multiple chain institutes shape of two planar emission apparatus (412) respectively from tissue culture bottle conveying device Into conveying circulation inside and outside synchronously closer or far from the conveying circulation the vertical plane by illumination.
2. a kind of LED light incubator according to claim 1, it is characterised in that on the LED aluminum base plate (4122) Red LED lamp and blue LED lamp distribute alternately, and controller controls each red LED lamp and blue LED lamp individually to light or put out Go out.
3. a kind of LED light incubator according to claim 1, it is characterised in that the red LED lamp and blue-ray LED The power of lamp is 1W, and the red spectral band of red LED lamp is 450 ± 20nm, and the blue wave band of blue LED lamp is 660 ± 20nm.
4. a kind of LED light incubator according to claim 1, it is characterised in that the mobile base (413) is in plane Radiating wing plate (4123) side frames of light-emitting device (412) are provided with the fan that can promote the circulation circulation of casing (411) interior air-flow (419) ventilation fan (420) that can carry out that air-flow exchanges inside and outside casing (411), is installed on the casing (411).
CN201610472548.5A 2015-06-04 2015-06-04 Tissue culture system and its led light incubator Expired - Fee Related CN105993957B (en)

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CN201610442545.7A Expired - Fee Related CN106035089B (en) 2015-06-04 2015-06-04 Tissue culture system and its manually train seedling room
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