CN106106087A - A kind of high degeneration-resistant Fructus Fragariae Ananssae culture matrix - Google Patents
A kind of high degeneration-resistant Fructus Fragariae Ananssae culture matrix Download PDFInfo
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- CN106106087A CN106106087A CN201610499555.4A CN201610499555A CN106106087A CN 106106087 A CN106106087 A CN 106106087A CN 201610499555 A CN201610499555 A CN 201610499555A CN 106106087 A CN106106087 A CN 106106087A
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- fructus fragariae
- fragariae ananssae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
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- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention discloses a kind of high degeneration-resistant Fructus Fragariae Ananssae culture matrix, be prepared by the raw materials in: plant ash 26 29, residue of monosodium glutamate 15 18, potassium carbonate 78, ammonium dihydrogen phosphate 46, calcium acetate 24, SILICA FUME 13, trichoderma microbial inoculum 1.2 1.6, sunflower plate granulated slag 36 38, Periostracum Serpentis waste material 35 37, pepsin preparation 23,1 (3 dimethylamino-propyl) 3 ethyl-carbodiimide hydrochloride 1.4 1.6, N N-Hydroxysuccinimide 0.3 0.5,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate；The culture matrix of the present invention utilizes the biological antagonist activity of trichoderma, by with pathogen competition for nutrients and growing space and reach to suppress the purpose of growth of pathogen, calcium acetate collocation simultaneously SILICA FUME, potassium carbonate etc. can realize the efficient absorption of Fructus Fragariae Ananssae centering trace element by the complexation of element, serve raising plant vigor, strengthen the advantageous effects of Fructus Fragariae Ananssae resistance.
Description
Technical field
The present invention relates to nutrient matrix technical field, particularly relate to a kind of high degeneration-resistant Fructus Fragariae Ananssae culture matrix.
Background technology
Substrate culture is a kind of important cultural method in the current agriculture of China, woods, flower planting industry, is that one passes through base
Matter absorbs nutritional solution and a kind of Soilless Culture Methods of oxygen, owing to it is low to soil environment requirement, equipment simple, water-saving saves fertilizer etc.
Advantage, is adopted application by vast plant development person.And continuous along with the development of China's plant husbandry and cultivated area
Expanding, the usage amount of cultivation matrix the most constantly increases, and traditional uses the single raw material such as the peat composed of rotten mosses, Vermiculitum or be simply mixed preparation
Substrate can not meet the demand of plantation, and the universal nutrition of present stage substrate simultaneously is single, causes sending out of plant in cultivation
Bud rate is low, survival rate is low, the physical instability of substrate during simultaneously using, and water absorption is poor, easily subside, surcharge
Low, it is impossible to realize higher using value for specific environment, affect the growth of plantation economy benefit.
Collagen protein, as a kind of biomacromolecule, has good pore structure after being modified, the most raw
Thing degradability, mechanical strength and biocompatibility, have good cell growth interface, can become a kind of cultivation matrix and build
In quality raw materials.China Serpentis aboundresources simultaneously, owing to the decorative pattern of Periostracum Serpentis outward appearance is the most gorgeous, pattern is unique, is used for making
The artware of various finenesses, but due to the backwardness of Periostracum Serpentis Technology, the course of processing creates a large amount of waste material, account for complete
The 20-30% of Periostracum Serpentis, if not being effectively treated, not only polluting environment, also wasting substantial amounts of raw material, containing substantial amounts of in Periostracum Serpentis
Collagen protein, this also applies for it and provides cheap raw material basis in substrate preparation.
The application for a patent for invention of Publication No. CN1702061A discloses the full nutrient matrix of a kind of botanical seedling culturing and cultivation,
This nutrient matrix use weight ratio be the peat of 50-70%, the perlite of 20-25%, the Vermiculitum of 10-15 and active substance,
Nutrient substance mixes, and has more comprehensively nutrients structure, but the cultivation matrix of this disclosure of the invention is due to size not
One, easily cause sedimentation after watering, cause the blocking of air in substrate, the crude fiber content in substrate is low simultaneously, needs extra battalion
Support the interpolation of material, improve production cost, and not for the additional use value of the real needs raising substrate cultivated, limit
Make the scale application of product.
Summary of the invention
The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of high degeneration-resistant Fructus Fragariae Ananssae culture matrix.
The present invention is achieved by the following technical solutions:
A kind of high degeneration-resistant Fructus Fragariae Ananssae culture matrix, is prepared by the raw materials in: plant ash 26-29, residue of monosodium glutamate 15-
18, potassium carbonate 7-8, ammonium dihydrogen phosphate 4-6, calcium acetate 2-4, SILICA FUME 1-3, trichoderma microbial inoculum 1.2-1.6, sunflower plate granulated slag
36-38, Periostracum Serpentis waste material 35-37, pepsin preparation 2-3,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride
1.4-1.6, N-hydroxy-succinamide 0.3-0.5,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is appropriate, water
In right amount.
Specifically comprising the following steps that of described height degeneration-resistant Fructus Fragariae Ananssae culture matrix preparation method
(1) plant ash, residue of monosodium glutamate, the mixing of trichoderma microbial inoculum are placed in fermentation vat, add water to ferment heap maximum water holding capacity
76-81%, ferments 23-26 days, by fermentation material squeeze and filter after having fermented, obtains fermentation liquid and ferment slag is standby, simultaneously will be to day
Certain herbaceous plants with big flowers dish cuts off, it is standby to pulverize as smalls；
(2) 4% sodium chloride solution is added by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour
Centrifugal 12-15 minute with 5800-6200 rev/min, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with
0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C,
Insulation is extracted 18-20 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 8 minutes, then with 5000-5500 rev/min of centrifugal 10-
12 minutes, collect the supernatant, obtain collagen solution standby；
(3) in step 1 gained fermentation liquid, add potassium carbonate, ammonium dihydrogen phosphate, calcium acetate, be heated to 55-60 DEG C, reaction
After 23-27 minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, SILICA FUME mixing for standby use；
(4) step 2 gained collagen solution is joined in step 3 gained material, is placed under 4-5 DEG C of low temperature environment,
After stirring 5-10 minute add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide with
And other leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-33 DEG C of drying, obtains substrate of the present invention.
The invention have the advantage that
Substrate of the present invention is by the extraction to Periostracum Serpentis, and by having reduced immunogenicity, the collagen protein of biodegradability should
Use in the preparation of cultivation matrix, utilize the mechanical performance of cross-linking modified raising albumen, it is achieved its support in substrate builds
Effect, can not only provide certain support and growth interface, the collagenous fiber bundle phase within collagen protein simultaneously for seed seedling
Be staggered porous network structure mutually that formed, is also beneficial to beneficial microorganism and carries and the extension of root cell, and is
It provides sufficient oxygen and nutrient substance, promotes the growth of plant, and culture matrix plays fixation, convenient transport.
The culture matrix of the present invention utilizes the biological antagonist activity of trichoderma, by empty with pathogen competition for nutrients and growth
Between and reach to suppress the purpose of growth of pathogen, calcium acetate collocation simultaneously SILICA FUME, potassium carbonate etc. can be by the complexations of element
Realize the efficient absorption of Fructus Fragariae Ananssae centering trace element, serve raising plant vigor, strengthen the advantageous effects of Fructus Fragariae Ananssae resistance.
Detailed description of the invention
A kind of high degeneration-resistant Fructus Fragariae Ananssae culture matrix, is made up of the raw material of following weight portion (kg): plant ash 26, residue of monosodium glutamate 15,
Potassium carbonate 7, ammonium dihydrogen phosphate 4, calcium acetate 2, SILICA FUME 1, trichoderma microbial inoculum 1.2, sunflower plate granulated slag 36, Periostracum Serpentis waste material 35,
Pepsin preparation 2,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride 1.4, N N-Hydroxysuccinimide 0.3,
4% sodium chloride solution is appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate.
Specifically comprising the following steps that of described height degeneration-resistant Fructus Fragariae Ananssae culture matrix preparation method
(1) plant ash, residue of monosodium glutamate, the mixing of trichoderma microbial inoculum are placed in fermentation vat, add water to ferment heap maximum water holding capacity
76%, ferment 23 days, by fermentation material squeeze and filter after having fermented, obtain fermentation liquid and ferment slag is standby, sunflower plate is cut simultaneously
Disconnected, pulverizing is that smalls is standby；
(2) add 4% sodium chloride solution by 1:16g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6 hours with
5800 revs/min are centrifuged 12 minutes, take precipitation clear water and clean and dry at 30 DEG C, then will precipitation and 0.15mol/L citric acid
Solution presses 1:8g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30 DEG C, and insulation is extracted 18 hours, after completing
It is heated to 85 DEG C, is incubated 8 minutes, then be centrifuged 10 minutes with 5000 revs/min, collect the supernatant, obtain collagen solution
Standby；
(3) in step 1 gained fermentation liquid, add potassium carbonate, ammonium dihydrogen phosphate, calcium acetate, be heated to 55 DEG C, react 23
After minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, SILICA FUME mixing for standby use；
(4) step 2 gained collagen solution is joined in step 3 gained material, be placed under 4 DEG C of low temperature environments, stir
After mixing 5 minutes add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N N-Hydroxysuccinimide and other
Leftover materials, cross-linking reaction 8 hours, after completing, material is warming up to 30 DEG C of drying, obtains substrate of the present invention.
In order to further illustrate the using value of the present invention, inventor have chosen respectively substrate of the present invention and commercially available commonly
Culture matrix carries out the cultivating and growing of Fructus Fragariae Ananssae, and during plantation, management method is identical, and during plantation, the physical and chemical index to substrate is carried out
Detection, result is as follows:
| Full nitrogen (%) | Full phosphorus (%) | Full potassium (%) | Total porosity (%) | Saturation moisture content (%) | |
| Substrate of the present invention | 0.958 | 0.12 | 0.45 | 89.02 | 897 |
| Commercially available generic media | 0.732 | 0.11 | 0.41 | 85.83 | 437 |
Substrate the most of the present invention plantation gained Yield of Strawberry relatively generic media improves more than 10%, and disease incidence reduces
Within 3%, it can be seen that substrate of the present invention relatively generic media is the most sufficient, simultaneously stable in physicochemical property, structure
Excellent, there is preferable prospect of the application.
Claims (2)
1. one kind high degeneration-resistant Fructus Fragariae Ananssae culture matrix, it is characterised in that be prepared by the raw materials in: plant ash 26-29, taste
Essence slag 15-18, potassium carbonate 7-8, ammonium dihydrogen phosphate 4-6, calcium acetate 2-4, SILICA FUME 1-3, trichoderma microbial inoculum 1.2-1.6, Xiang
Certain herbaceous plants with big flowers dish granulated slag 36-38, Periostracum Serpentis waste material 35-37, pepsin preparation 2-3,1-(3-dimethylamino-propyl)-3-ethyl carbodiimide
Hydrochlorate 1.4-1.6, N-hydroxy-succinamide 0.3-0.5,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is fitted
Amount, water are appropriate.
2. according to the height degeneration-resistant Fructus Fragariae Ananssae culture matrix described in claims 1, it is characterised in that the concrete steps of preparation method are such as
Under:
(1) plant ash, residue of monosodium glutamate, the mixing of trichoderma microbial inoculum are placed in fermentation vat, add water to the 76-of ferment heap maximum water holding capacity
81%, ferment 23-26 days, by fermentation material squeeze and filter after having fermented, obtain fermentation liquid and ferment slag is standby, simultaneously by sunflower plate
Cut off, pulverize as smalls standby；
(2) add 4% sodium chloride solution by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour with
5800-6200 rev/min is centrifugal 12-15 minute, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with
0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C,
Insulation is extracted 18-20 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 8 minutes, then with 5000-5500 rev/min of centrifugal 10-
12 minutes, collect the supernatant, obtain collagen solution standby；
(3) in step 1 gained fermentation liquid, add potassium carbonate, ammonium dihydrogen phosphate, calcium acetate, be heated to 55-60 DEG C, react 23-
After 27 minutes cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, SILICA FUME mixing for standby use；
(4) step 2 gained collagen solution is joined in step 3 gained material, be placed under 4-5 DEG C of low temperature environment, stirring
After 5-10 minute add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide and its
His leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-33 DEG C of drying, obtains substrate of the present invention.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610499555.4A CN106106087A (en) | 2016-06-28 | 2016-06-28 | A kind of high degeneration-resistant Fructus Fragariae Ananssae culture matrix |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610499555.4A CN106106087A (en) | 2016-06-28 | 2016-06-28 | A kind of high degeneration-resistant Fructus Fragariae Ananssae culture matrix |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102716516A (en) * | 2012-05-11 | 2012-10-10 | 天津大学 | Polydatin modified collagen scaffold, and preparation method and application thereof |
| CN103159967A (en) * | 2013-04-11 | 2013-06-19 | 江南大学 | Preparation method of collagen-based sponge wound dressing with self-anti-inflammatory function |
| CN103627761A (en) * | 2013-10-22 | 2014-03-12 | 浙江省海洋开发研究院 | Method for preparing collagen peptide rich in hydroxyproline |
| CN103961749A (en) * | 2014-05-07 | 2014-08-06 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein/silica membrane double-layer stent |
| CN104447092A (en) * | 2014-12-03 | 2015-03-25 | 大新县生产力促进中心 | Special composite fertilizer for broadleaf holly leaves and production method of composite fertilizer |
| CN104744107A (en) * | 2013-12-25 | 2015-07-01 | 上海宇强贸易有限公司 | Biological seedling growing matrix |
| CN104825385A (en) * | 2015-04-22 | 2015-08-12 | 天津大学 | Collagen-based nanometer silver antibacterial support and its preparation method and use |
-
2016
- 2016-06-28 CN CN201610499555.4A patent/CN106106087A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102716516A (en) * | 2012-05-11 | 2012-10-10 | 天津大学 | Polydatin modified collagen scaffold, and preparation method and application thereof |
| CN103159967A (en) * | 2013-04-11 | 2013-06-19 | 江南大学 | Preparation method of collagen-based sponge wound dressing with self-anti-inflammatory function |
| CN103627761A (en) * | 2013-10-22 | 2014-03-12 | 浙江省海洋开发研究院 | Method for preparing collagen peptide rich in hydroxyproline |
| CN104744107A (en) * | 2013-12-25 | 2015-07-01 | 上海宇强贸易有限公司 | Biological seedling growing matrix |
| CN103961749A (en) * | 2014-05-07 | 2014-08-06 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein/silica membrane double-layer stent |
| CN104447092A (en) * | 2014-12-03 | 2015-03-25 | 大新县生产力促进中心 | Special composite fertilizer for broadleaf holly leaves and production method of composite fertilizer |
| CN104825385A (en) * | 2015-04-22 | 2015-08-12 | 天津大学 | Collagen-based nanometer silver antibacterial support and its preparation method and use |
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| CB03 | Change of inventor or designer information |
Inventor after: Zhang Dianxing Inventor after: Huo Mingqin Inventor after: Qian Xiaoqiang Inventor after: Lan Wei Inventor after: Zhang Haitao Inventor before: Zhang Dianxing Inventor before: Huo Mingqin |
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| COR | Change of bibliographic data | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161116 |
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| RJ01 | Rejection of invention patent application after publication |