CN106069670A - Drupe fruit tree and the tender slightly cottage method of stock - Google Patents
Drupe fruit tree and the tender slightly cottage method of stock Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses drupe fruit tree and the tender slightly cottage method of stock, including cultivating, there is the cutting of strong meristematic capacity, the process of cutting, tender slightly cuttage.Cottage method of the present invention, first makes treelet and tender slightly holding plant tissue active state;By continuous pinching, spray the basic element of cell division and naphthalene acetic acid after pinching, and again spray before adopting fringe, make histiocyte maintain ' young ' active state, add tree somatic cell division cellulose content, easily form callus, increase content of material of taking root, make to take root easier, rootage duration shifts to an earlier date 5~7d, rooting rate has reached more than 97%, and emergence rate significantly improves, and economic benefit improves more than 80%;This method management difficulty is low, saves labour force, has the features such as simple to operate, easy popularization.
Description
Technical field
The present invention relates to a kind of cuttage seedling raising method, be specifically related to drupe fruit tree and the tender slightly cottage method of stock, belong to plant
Reproduction technique field.
Background technology
Cuttage is a traditional plant asexual multiplication technology, and Studies on Fruit Cutting Propagation just had begun to before 2000 should
With, domestic through development for many years, cuttage technique reaches its maturity, in recent years, along with auximone and auto-spraying system device
Application, its development is maked rapid progress.Tone fruit trees abundant species, including Fructus Persicae, Lee, Fructus Pruni, Fructus Pruni pseudocerasi etc., its main modes of reproduction is transferred
Connect breeding, cottage propagation etc..Cuttage is also used for stock breeding.At present, the cuttage research of China tone fruit trees and stock also exists
Constantly among progress, existing cottage method mainly uses semi-lignified cuttage, but has the disadvantage that
(1) the drupe fruit tree such as cottage propagation rooting rate such as Fructus Persicae, Fructus Pruni, Lee, Fructus Pruni pseudocerasi is low, and some kind is even difficult to take root.
(2) existing cuttage technique is mainly to cut branch and is cut into segment cuttage in substrate, different parts cuttage rooting difficulty or ease
Degree is different, causes cuttage rooting time difference relatively big, causes management difficulty to strengthen.
(3) existing cuttage technique cutting meristematic capacity is weak, and auxin content is low, takes root relatively slow, and the high temperature of environment is high
Wet it be easily caused in substrate the breeding quickening of various pathogen and cause cutting bottom to be rotted, have impact on cutting survival rate.
Summary of the invention
The technical problem to be solved in the present invention is for above not enough, it is provided that drupe fruit tree and the tender slightly cottage method of stock, logical
Cross continuous pinching and spray the series of measures such as the basic element of cell division and auxin, make tender slightly organize to be in all the time enliven mitogenetic shape
State, auxin content is high, it is achieved following goal of the invention:
(1) cottage method of the present invention, cottage propagation rooting rate is high;
(2) cottage method of the present invention, cuttage rooting concordance is good;
(3) cottage method of the present invention, shortens rootage duration, improves cutting propagation rooting rate and emergence rate, improves surviving of cutting
Rate;
(4) cottage method of the present invention, reduces seedling cost, improves market supply ability.
For solving above-mentioned technical problem, by the following technical solutions:
Drupe fruit tree and the tender slightly cottage method of stock, it is characterised in that: include cultivating there is the cutting of strong meristematic capacity, cutting
Process, tender slightly cuttage.
The following is and the further of technique scheme is improved:
Described cultivation has the cutting of strong meristematic capacity, chooses tissue culture seedling as adopting fringe strain;Described seedling branch growth
To pinching when 30~50cm, and pinching continuously.
Described cultivation has the cutting of strong meristematic capacity, spray in 1~3d after continuous pinching the basic element of cell division 20~
100ppm adds naphthalene acetic acid 50~150ppm mixed solution, sprays more than 2 times continuously.
It is described that to adopt fringe strain be 1~3 year raw tree.
Described cultivation has the cutting of strong meristematic capacity, and continuous pinching more than 2 times, young sprout length is to more than 30cm, then sprays
The basic element of cell division 20~100ppm adds naphthalene acetic acid 50~150ppm mixed solution, and after spraying, a 3~5d interior clip shoot apex are tender
Slightly as cutting.
The process of described cutting: the every segment length of cutting 15~25cm, retains top and top 5~6 blades, rest blade
Extracing, cutting lower cut is tiltedly cut domatic about the 1-2cm of reservation and lower end is cut flat;Base diameter 0.2-0.5cm of cutting.
The process of described cutting, the carbendazim that cutting mass ratio is 2:1 after cutting and thiophanate methyl wettable powder
Agent 800~2000 times of immersions bubble 5~8min carry out disinfection, then with concentration than be 2:1 NAA+IBA root-growing agent 300~
600ppm solution soaking cutting base portion is at 2~3cm 5~10min.
Described tender slightly cuttage, cuttage seeding row spacing is 10-15cm × 12-20cm, and the cuttage degree of depth is 6~8cm;After cuttage in canopy
Air humidity is maintained at 85%-95%, and temperature controls at 20-32 DEG C, less than less than 35 DEG C.
Described tender slightly cuttage, cutting medium uses perlite and the peat composed of rotten mosses, is mixed for 1:1 by volume, with 0.1%
Divide after potassium permanganate solution spray disinfectant in slotting machine, stromal thickness 15cm, irrigate with clear water.
Described tender slightly cuttage, cutting medium is with weight portion, including following components:
Meerschaum 30 parts, 10 parts of D-alkene, carboxymethyl cellulose 4 parts, star shellfish Sargassum fusiforme (Harv.) Setch 5 parts, Rhizoma amorphophalli powder 6 parts, amino-oligosaccharide
0.5 part, biochemical yellow humic acid 12 parts, Huai Goat 5 parts, graphene oxide 2 parts.
At present, cuttage technique uses cutting to be semi-lignified branch, and rooting rate is low, and this technology improves rooting rate and mainly arranges
Execute and be: 1. selecting tissue cultured seedling to build cutting orchard, only adopt fringe from 1~3 year raw tree, treelet can keep plant tissue active state;②
Pinching makes young sprout then maintain the tender and easy differentiation state of cell of children continuously;3. young sprout is used to replace original semi-wooden scion, newly
Tip tissue is in mitogenetic state, is conducive to taking root;4. spray the basic element of cell division and naphthalene acetic acid, and again spray before adopting fringe, make
Histiocyte maintains ' young ' active state, increases tree somatic cell division cellulose content, easily forms callus, increases thing of taking root
Matter content, makes to take root easier.Above-mentioned measure is with the use of embodying good result.
Compared with prior art, the invention has the beneficial effects as follows:
(1) cutting propagation of the present invention, rooting rate significantly improves, and as a example by Fructus Persicae new varieties ' the U.S. moon ', rooting rate reaches more than 97%, often
Rule semi-lignified cutting plantation is only 15%, improves 82 percentage points, and rooting rate improves more than 6 times;
(2) cutting propagation of the present invention, tender slightly tissue is in mitogenetic state of enlivening, and auxin content is high, it is achieved that cuttage rooting
Concordance, to 25d, major part cutting is taken root;
(3) cutting propagation of the present invention, shortens rootage duration, shortens 5~7d than the conventional semi-lignified cuttage root-taking time;
(4) cutting propagation of the present invention, emergence rate significantly improves, and spring in next year investigates emergence rate, reaches 97.7-98%, more wooden than half
Change cuttage and improve 40.7-41.5 percentage point;Economic benefit improves more than 80%;
(5) problem that present invention, avoiding that common cuttage root-taking is slow and cutting rots;
(6) method of the present invention, management difficulty is low, has the feature of popularization simple to operate, easy.
Detailed description of the invention
The detailed description of the invention of the present invention is described with concrete case study on implementation below:
Embodiment 1 drupe fruit tree and the tender slightly cottage method of stock
Illustrate as a example by peach variety ' the U.S. moon ', test by the present embodiment in proving ground, academy of agricultural sciences of Weifang City,
Test and set process group and matched group:
Process group: the cottage method of the employing present invention:
Comprise the following steps:
(1) cultivation has the cutting of strong meristematic capacity
Choose tissue culture's Seedling establishment cutting orchard, pinching during seedling branch growth to 30cm, and pinching 2 times continuously, pluck new
Tip top 5cm, sprays basic element of cell division 60ppm in 1~3d after pinching and adds naphthalene acetic acid 80ppm mixed solution, spray continuously 2 times,
The holding of tender slightly tissue is made to enliven mitogenetic state.
Pinching 2 times, the young sprout length that secondary grows to more than 30cm, spray basic element of cell division 60ppm add naphthalene acetic acid 80ppm mix
Closing solution, in spraying rear 3d, clip shoot apex is tender slightly as cutting, timely cuttage.
(2) the purchasing of slotting machine and substrate
Cuttage uses auto-spraying system system temperature control moisturizing in using hot house, canopy, covers the sunshade that shading rate is 70% outside canopy film
Net, slotting machine south-north direction, long 50m width 1.2m;Cutting medium uses perlite and the peat composed of rotten mosses, is mixed for 1:1 by volume, uses
Divide in slotting machine after the potassium permanganate solution spray disinfectant of 0.1%, stromal thickness 15cm, irrigate with clear water.
(3) process of cutting
Cutting every segment length 15cm, retains top and 5, top blade, and rest blade is extractd, and it is domatic that cutting lower cut tiltedly cuts reservation
About 1cm also cuts flat lower end.Cut rear cutting carbendazim and thiophanate methyl wettable powder (mass ratio is 2:1) 1500 times
Immersion bubble 5min carries out disinfection, and is then 2:1 with NAA+IBA(concentration ratio) root-growing agent 500ppm solution soaking cutting base portion is extremely
5min at 2cm;
Base diameter 0.2cm of cutting.
(4) cuttage and management
Cuttage seeding row spacing is 10cm × 12cm, and isosceles triangle is distributed, cuttage degree of depth 6cm;After cuttage, in canopy, air humidity is maintained at
85%-95%, temperature controls at 20-32 DEG C, less than 35 DEG C.
Matched group, traditional semi-lignified cuttage
Select to grow and enrich, give birth to semi-lignified branch then without pest and disease damage, cut the section of being cut into, every segment length 15~20cm, reservation 5~
6 blades;Its upper cut truncation, away from upper bud about 0.5~1cm, lower cut is cut sth. askew, and cutting carbendazim is wettable with thiophanate methyl
Property powder (mass ratio is 2:1) 1500 times of immersions bubble 6min carry out disinfection, be then 2:1 with NAA+IBA(concentration ratio) root-growing agent
500ppm solution soaking cutting base portion is to 8min at 2cm.After cuttage, in canopy, air humidity controls 75%~95%, and temperature controls
22 DEG C~32 DEG C, water content of substrate controls 50%~60%.Week about, to cutting 25% carbendazol wettable powder 300 times
Liquid and 70% 1500 times of liquid of first torr wettable powder alternately use pre-disease prevention.
Result of the test:
The cuttage seedling raising method cutting 6d using the present invention has callus to produce, and conventional semi-lignified cuttage has wound healing on the 8th day
Tissue produces;
16d part cutting bears adventitious root, compared with conventional semi-lignified cuttage, takes root and is advanced by 7d;
To 25d, cutting is taken root, and rooting rate has reached 97.4%, and rotting rate 1.2%, than the raising of semi-lignified cutting plantation
83 percentage points;
Spring in next year investigates emergence rate, reaches 98%, improves 41.5 percentage points than semi-lignified cuttage.
Embodiment 2 drupe fruit tree and the tender slightly cottage method of stock
Illustrate with peach variety ' Xia Dan ', test by the present embodiment in proving ground, academy of agricultural sciences of Weifang City, test
If process group and matched group:
Process group: the cuttage of the employing present invention:
(1) cultivation has the cutting of strong meristematic capacity
Choose tissue culture's Seedling establishment cutting orchard, pinching during seedling branch growth to 50cm, and pinching 2 times continuously, pluck new
Tip top 5cm, sprays basic element of cell division 50ppm and adds naphthalene acetic acid 80ppm mixed solution, spray continuously 2 times, make tender in 3d after pinching
Slightly tissue holding enlivens mitogenetic state.
Pinching 2 times, young sprout length, to more than 30cm, sprays basic element of cell division 50ppm and adds naphthalene acetic acid 80ppm mixed solution, spray
In executing rear 5d, clip shoot apex is tender slightly as cutting, timely cuttage.
(2) the purchasing of slotting machine and substrate
Cuttage uses auto-spraying system system temperature control moisturizing in using hot house, canopy, covers the sunshade that shading rate is 90% outside canopy film
Net, slotting machine south-north direction, long 50m width 1.2m;Cutting medium uses perlite and the peat composed of rotten mosses, is mixed for 1:1 by volume, uses
Divide in slotting machine after the potassium permanganate solution spray disinfectant of 0.1%, stromal thickness 15cm, irrigate with clear water.
(3) process of cutting
Cutting every segment length 25cm, retains top and 6, top blade, and rest blade is extractd, and it is domatic that cutting lower cut tiltedly cuts reservation
About 1-2cm also cuts flat lower end.Cut rear cutting carbendazim and thiophanate methyl wettable powder (mass ratio is 2:1) 1500
Times immersion bubble 8min carries out disinfection, and is then 2:1 with NAA+IBA(concentration ratio) root-growing agent 600ppm solution soaking cutting base portion is extremely
10min at 3cm;
Base diameter 0.5cm of cutting.
(4) cuttage and management
Cuttage seeding row spacing is 15cm × 20cm, and isosceles triangle is distributed, cuttage degree of depth 8cm;After cuttage, in canopy, air humidity is maintained at
More than 85%-95%, temperature controls at 20-32 DEG C, it is impossible to more than 35 DEG C.
Matched group, traditional semi-lignified cuttage
Select to grow and enrich, give birth to semi-lignified branch then without pest and disease damage, cut the section of being cut into, every segment length 15~20cm, reservation 5~
6 blades;Its upper cut truncation, away from upper bud about 0.5~1cm, lower cut is cut sth. askew, and cutting carbendazim is wettable with thiophanate methyl
Property powder (mass ratio is 2:1) 1500 times of immersions bubble 6min carry out disinfection, be then 2:1 with NAA+IBA(concentration ratio) root-growing agent
500ppm solution soaking cutting base portion is to 8min at 2cm.After cuttage, in canopy, air humidity controls 75%~95%, and temperature controls
22 DEG C~32 DEG C, water content of substrate controls 50%~60%.Week about, to cutting 25% carbendazol wettable powder 300 times
Liquid and 70% 1500 times of liquid of first torr wettable powder alternately use pre-disease prevention.
Result of the test:
The cuttage seedling raising method cutting 6d using the present invention has callus to produce, and conventional semi-lignified cuttage has wound healing on the 7th day
Tissue produces;
16d part cutting bears adventitious root, compared with conventional semi-lignified cuttage, takes root and is advanced by 5d;
To 25d, major part cutting is taken root, and rooting rate has reached 98.1%, and rotting rate 1.0%, than conventional semi-lignified cuttage
Rooting rate improves 82.5 percentage points;
Spring in next year investigates emergence rate, reaches 97.7%, improves 40.7 percentage points than common cuttage.
Tender slightly cuttage and seedling culture method described in embodiment 1,2, tender slightly tissue is in mitogenetic state of enlivening, auxin content
High, it is achieved that the concordance of cuttage rooting, early, rooting rate is high for rootage duration, it is to avoid common cuttage root-taking is slow and cutting rots
Problem;This method saves labour force, simple to operate, easily promote.
Embodiment 3 drupe fruit tree and the tender slightly cottage method of stock
Illustrate with peach variety ' Xia Dan ', test by the present embodiment in proving ground, academy of agricultural sciences of Weifang City, test
If process group and matched group:
Process group: the cuttage of the employing present invention:
(1) cultivation has the cutting of strong meristematic capacity
Choose 2 years raw Seedling establishment cutting orchards of peach tree tissue culture, pinching during seedling branch growth to 50cm, and pinching 2 continuously
Secondary, pluck young sprout top 5cm, spray basic element of cell division 50ppm after pinching in 3d and add naphthalene acetic acid 80ppm mixed solution, spray continuously
Execute 2 times, make the holding of tender slightly tissue enliven mitogenetic state.
Pinching 2 times, young sprout length, to more than 30cm, sprays basic element of cell division 50ppm and adds naphthalene acetic acid 80ppm mixed solution, spray
In executing rear 5d, clip shoot apex is tender slightly as cutting, timely cuttage.
(2) the purchasing of slotting machine and substrate
Cuttage uses auto-spraying system system temperature control moisturizing in using hot house, canopy, covers the sunshade that shading rate is 90% outside canopy film
Net, slotting machine south-north direction, long 50m width 1.2m;
Cutting medium, with weight portion, including following components:
Meerschaum 30 parts, 10 parts of D-alkene, carboxymethyl cellulose 4 parts, star shellfish Sargassum fusiforme (Harv.) Setch 5 parts, Rhizoma amorphophalli powder 6 parts, amino-oligosaccharide
0.5 part, biochemical yellow humic acid 12 parts, Huai Goat 5 parts, graphene oxide 2 parts.
Described meerschaum, 200 mesh, powder, specific surface area is 250m2/g, and pore volume is 0.385ml/g, and cation exchanges
Amount is every 100g dry ground about 35mg equivalent cation;Dioxide-containing silica is 58%, and content of magnesia is 23%, absorbs more than self
The water of weight 155%;
Described Rhizoma amorphophalli powder, glucomannoglycan content reaches 60%;
Described amino-oligosaccharide, active constituent content is 5%
Described Huai Goat, the content of rutin is 96%, the content of Quercetin is 2%, the content of rhamnose is 0.5%.
Described yellow humic acid, brownish-yellow powder, 100-200 mesh;In terms of butt, active constituent content >=90%;Water solublity >=
99%, amino acid content >=5%, GA content 0.05%, pH is 5.5, moisture≤1.8%;
Described graphene oxide, for pulverulence, specific surface area >=1800m2/g, aperture is 5-8nm, D10≤20 μm, D50≤
60 μm, D90≤80 μm, purity >=99wt%, ash≤0.5wt%, moisture≤0.6wt%.
The preparation method of described cutting medium, by star shellfish Sargassum fusiforme (Harv.) Setch, be soaked in water 1h, and the star shellfish sheep after then soaking dwells
Dish, dries, and carries out fermentation maturity, then with meerschaum, D-alkene, carboxymethyl cellulose, Rhizoma amorphophalli powder, amino-oligosaccharide, the Flos Sophorae Immaturus
The mix homogeneously such as extract, graphene oxide, prepares cutting medium.
Described cutting medium is divided in slotting machine with after the potassium permanganate solution spray disinfectant of 0.1%, stromal thickness
15cm, irrigates with clear water.
(3) process of cutting
Cutting every segment length 25cm, retains top and 6, top blade, and rest blade is extractd, and it is domatic that cutting lower cut tiltedly cuts reservation
About 1-2cm also cuts flat lower end;To take root disinfectant, be diluted to 600ppm solution, soak cutting base portion to 10min at 3cm;
Base diameter 0.5cm of cutting.
Described disinfectant of taking root, in parts by weight, including following components:
Naphthalene acetic acid, coptisine 4.2 parts, Huai Goat 13 parts, Aloe extract 6 parts, tea tree oil 5.5 parts, fulvic acid 3 parts, sulfur
Ferrous 1 part of acid, calcium amino acid chelate 4 parts, amino-oligosaccharide 3 parts.
Described calcium amino acid chelate, calcium content is 9-11%;Moisture≤5%, content of ashes≤2.5%, aspartic acid
Content be 2.1-2.3%, threonine content is 1.9-2.1%, and serine content is 2.6-2.8%, and content of glutamic acid is 5.2-
5.4%。
In described Huai Goat the content of rutin be 96%, the content of Quercetin be 2%, the content of rhamnose be 0.5%.
Described Aloe extract, the content of aloe-emodin is 96%, the content of Aloe chrysophanol is 1%, aloe saponaria glycosides
Content be 1%, the content of Aloearbonaside be 1%;
Described amino-oligosaccharide, active constituent content is 5%.
(4) cuttage and management
Cuttage seeding row spacing is 15cm × 20cm, and isosceles triangle is distributed, cuttage degree of depth 8cm;After cuttage, in canopy, air humidity is maintained at
More than 85%-95%, temperature controls at 20-32 DEG C, it is impossible to more than 35 DEG C.
Matched group, traditional semi-lignified cuttage
Select to grow and enrich, give birth to semi-lignified branch then without pest and disease damage, cut the section of being cut into, every segment length 15~20cm, reservation 5~
6 blades;Its upper cut truncation, away from upper bud about 0.5~1cm, lower cut is cut sth. askew, and cutting carbendazim is wettable with thiophanate methyl
Property powder (mass ratio is 2:1) 1500 times of immersions bubble 6min carry out disinfection, be then 2:1 with NAA+IBA(concentration ratio) root-growing agent
500ppm solution soaking cutting base portion is to 8min at 2cm.After cuttage, in canopy, air humidity controls 75%~95%, and temperature controls
22 DEG C~32 DEG C, water content of substrate controls 50%~60%.Week about, to cutting 25% carbendazol wettable powder 300 times
Liquid and 70% 1500 times of liquid of first torr wettable powder alternately use pre-disease prevention.
Result of the test:
The cuttage seedling raising method cutting 4d using the present invention has callus to produce, and conventional semi-lignified cuttage has wound healing on the 7th day
Tissue produces;
14d part cutting bears adventitious root, compared with conventional semi-lignified cuttage, takes root and is advanced by 7d;
To 25d, major part cutting is taken root, and rooting rate has reached 99.0%, and rotting rate is 0.5%;Adventitious root increasing number
20%;Main root length increases by 10%;
Spring in next year investigates emergence rate, reaches 98.9%;The sickness rate of the pest and disease damage of peach seedling is few,
The anthracnose of peach of peach seedling, peach brown rot crown gall sickness rate low, respectively 0.4%, 0.3%, 0.6%;
The insect pest of peach seedling is few, and peach leafminer noth, the incidence rate of black peach aphid are respectively 1.2%, 0.9%;
After cuttage 1 year, branch and leaf amount relatively compares increase by 30%;The plant height of cuttage peach seedling relatively compares increase 10cm, and base diameter increases
0.2cm。
1 year raw cutting seedling of the present invention, after transplanting, survival rate 99.7%, disease and insect resistance, pest and disease damage incidence rate is 0.5%, raw
Long speed is fast, and base diameter relatively compares, and growth rate improves 17%;Result time advance 15 days.
Unless specifically indicated, ratio of the present invention is mass ratio, described percent, is percent mass
Number.
Finally it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention,
Although being described in detail the present invention with reference to previous embodiment, for a person skilled in the art, it still may be used
So that the technical scheme described in foregoing embodiments to be modified, or wherein portion of techniques feature is carried out equivalent.
All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included in the present invention's
Within protection domain.
Claims (10)
1. drupe fruit tree and the tender slightly cottage method of stock, it is characterised in that: include cultivating there is the cutting of strong meristematic capacity, cutting
Process, tender slightly cuttage.
Drupe fruit tree the most according to claim 1 and the tender slightly cottage method of stock, it is characterised in that: described cultivation has by force
The cutting of meristematic capacity, chooses tissue culture seedling as adopting fringe strain;Described seedling branch growth to pinching when 30~50cm, and
Pinching continuously.
Drupe fruit tree the most according to claim 2 and the tender slightly cottage method of stock, it is characterised in that: described cultivation has by force
The cutting of meristematic capacity, sprays the basic element of cell division 20~100ppm in 1~3d and adds naphthalene acetic acid 50~150ppm and mix after continuous pinching
Close solution, spray more than 2 times continuously.
Drupe fruit tree the most according to claim 2 and the tender slightly cottage method of stock, it is characterised in that adopting fringe strain described in: is 1
~3 years raw trees.
Drupe fruit tree the most according to claim 2 and the tender slightly cottage method of stock, it is characterised in that: described cultivation has by force
The cutting of meristematic capacity, continuous pinching more than 2 times, young sprout length is to more than 30cm, then sprays the basic element of cell division 20~100ppm and add
Naphthalene acetic acid 50~150ppm mixed solution, after spraying, a 3~5d interior clip shoot apex are tender slightly as cutting.
Drupe fruit tree the most according to claim 1 and the tender slightly cottage method of stock, it is characterised in that: the place of described cutting
Reason: the every segment length of cutting 15~25cm, retains top and top 5~6 blades, and rest blade is extractd, and cutting lower cut tiltedly cuts guarantor
Stay domatic about 1-2cm and lower end is cut flat;Base diameter 0.2-0.5cm of cutting.
Drupe fruit tree the most according to claim 1 and the tender slightly cottage method of stock, it is characterised in that: the place of described cutting
Reason, the carbendazim that cutting mass ratio is 2:1 after cutting and thiophanate methyl wettable powder 800~2000 times of immersion bubbles 5~
8min carries out disinfection, then with concentration than be NAA+IBA the root-growing agent 300~600ppm solution soaking cutting base portion of 2:1 to 2~
At 3cm 5~10min.
Drupe fruit tree the most according to claim 1 and the tender slightly cottage method of stock, it is characterised in that: described tender slightly cuttage,
Cuttage seeding row spacing is 10-15cm × 12-20cm, and the cuttage degree of depth is 6~8cm;After cuttage, in canopy, air humidity is maintained at 85%-
95%, temperature controls at 20-32 DEG C, less than less than 35 DEG C.
Drupe fruit tree the most according to claim 1 and the tender slightly cottage method of stock, it is characterised in that: described tender slightly cuttage,
Cutting medium uses perlite and the peat composed of rotten mosses, is mixed for 1:1 by volume, after the potassium permanganate solution spray disinfectant of 0.1%
Divide in slotting machine, stromal thickness 15cm, irrigate with clear water.
Drupe fruit tree the most according to claim 1 and the tender slightly cottage method of stock, it is characterised in that: described tender slightly cuttage,
Cutting medium is with weight portion, including following components:
Meerschaum 30 parts, 10 parts of D-alkene, carboxymethyl cellulose 4 parts, star shellfish Sargassum fusiforme (Harv.) Setch 5 parts, Rhizoma amorphophalli powder 6 parts, amino-oligosaccharide
0.5 part, biochemical yellow humic acid 12 parts, Huai Goat 5 parts, graphene oxide 2 parts.
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