CN103918430B

CN103918430B - A kind of styrax tonkinensis Craib ex Hart cuttage breeding method

Info

Publication number: CN103918430B
Application number: CN201410113329.9A
Authority: CN
Inventors: 李因刚; 柳新红; 朱光权; 石从广
Original assignee: Zhejiang Academy of Forestry
Current assignee: Zhejiang Academy of Forestry
Priority date: 2014-03-25
Filing date: 2014-03-25
Publication date: 2016-06-22
Anticipated expiration: 2034-03-25
Also published as: CN103918430A

Abstract

A kind of styrax tonkinensis Craib ex Hart cuttage breeding method, relates to a kind of cottage method。At present, styrax tonkinensis Craib ex Hart cuttage survival rate is low, have impact on the breeding of the high-volume of styrax tonkinensis Craib ex Hart high quality seedling。The present invention comprises the following steps: 1) fringe bar gathers and processes: to before sprouting, taking the branch of 1 year liftoff more than 0.2m of raw nursery stock, wipe out side shoot and only retain trunk, be cut to the brachyplast of 0.4～0.5m length depending on beam length, brachyplast two ends mouth of sealing with wax after fallen leaves；2) fringe bar cold preservation: after the binding of many fringe bars, wrap up with absorbent cotton, makes absorbent cotton moisten with aerosol apparatus water spray, is put into by the fringe bar wrapping up in outward absorbent cotton afterwards without fail in plastic bag and seal, be placed in 2～5 DEG C of low-temperature conservation cabinets are stored standby；3) seedbed prepares；4) cuttage cutting；5) control measures。The technical program cuttage survival rate is high, can reach 70%, and cost is low, it is easy to operation, it may be achieved the high-volume breeding of styrax tonkinensis Craib ex Hart high-quality clone nursery stock。

Description

A kind of styrax tonkinensis Craib ex Hart cuttage breeding method

Technical field

The present invention relates to a kind of cottage method。

Background technology

Styrax tonkinensis Craib ex Hart (Styraxtonkinensis) belongs to Styracaceae (Styracaceae) Styraax (Styrax) deciduous tree, heliophilous species, natural distributed is in China south China, southwest and extends to Vietnam and Laos, spans 3 temperature band such as north tropic, south subtropics and middle subtropical zone。Rapidly, the phase of becoming a useful person is short in growth, is the desirable seeds of pulpwood in ultrashort period of felling in turn；Seed kernel oil is based on unsaturated fatty acid, and total content reaches 85.4%, can produce biodiesel；Tree-like grace, floral white bunchiness, time in full bloom, full-blown flowers are like snow, great ornamental value；Its resin claims " Benzoinum ", has important medical value。Styrax tonkinensis Craib ex Hart as logging with, oil with, medicinal and the various value seeds such as view and admire, research was on the increase both at home and abroad in recent years。In breeding and cultivation, solve the technical problem such as seed seedling-raising, artificial forest cultivation, but vegetative propagation technique not yet obtains breakthrough。Asexual propagation can keep the merit of parent, plays key foundation effect in Forest tree cultivation and genetic improvement。Cuttage is as a kind of low cost, workable styrax tonkinensis Craib ex Hart vegetative propagation technique, not yet by systematic study and proposition, have impact on the breeding of the high-volume of styrax tonkinensis Craib ex Hart high quality seedling to a certain extent。

Summary of the invention

The technical assignment of the technical problem to be solved in the present invention and proposition is to carry out improving to prior art and improve, it is provided that a kind of styrax tonkinensis Craib ex Hart cuttage breeding method, to reach to improve the purpose of cuttage success rate。For this, the present invention takes techniques below scheme。

A kind of styrax tonkinensis Craib ex Hart cuttage breeding method, it is characterised in that comprise the following steps:

1) fringe bar gathers and processes: to before sprouting, taking the branch of 1 year liftoff more than 0.2m of raw nursery stock, wipe out side shoot and only retain trunk, be cut to the brachyplast of 0.4～0.5m length depending on beam length, brachyplast two ends mouth of sealing with wax after fallen leaves；

2) fringe bar cold preservation: after the binding of many fringe bars, wrap up with absorbent cotton, makes absorbent cotton moisten with aerosol apparatus water spray, is then put into by the fringe bar wrapping up in outward absorbent cotton in plastic bag and seal, be placed in 2～5 DEG C of low-temperature conservation cabinets are stored standby；

3) seedbed prepares: with cement pit for cuttage seedbed, and in bed, the paving thickness perlite more than 15cm is as substrate, peripheral hardware shed, and shed thin film covers, with the uniform spray disinfectant of potassium permanganate solution before cuttage；

4) cuttage cutting: mid-April, when mean temperature reaches more than 15 DEG C, takes out the fringe bar of storage, is cut into length 10cm～12cm, cutting with more than 1 bud；Bud is positioned at the upper position of cutting, cutting top lateral shear, lower end oblique shear；It is one group by some cutting, ties and be placed in the mixed solution containing GGR6, IBA prepared and soak 4-8 hour, soaking depth 4～5cm；First punch in substrate with the bamboo let thicker than cutting before cuttage, the jack degree of depth 8～10cm, density be 10cm × 10cm or more than；Putting into compacting substrate after cutting, after having inserted, water spray is saturated to substrate, is then built by shed；

5) control measures: keep the corresponding humidity of substrate after cuttage, when temperature reaches 30 DEG C, ventilation and heat is opened at shed two；Transplant after having more fibrous root to grow。

Carry out cuttage when mid-April, now temperature is high, and cytoactive is strong, and callus and root growth time can effectively shorten, and reduces the mildew and rot phenomenon of cutting that in management process, unfavorable factor causes。Take off and put in freezer before cutting is sprouted so that it is in a dormant state。

As improving further and supplementing technique scheme, present invention additionally comprises following additional technical feature。

The mass percent concentration of GGR6, IBA respectively 300mg L in mixed solution^-1、100mg·L^-1。GGR6, IBA are all mainly used in taking root；Both mixing improve the rooting rate of cutting。

Cut-off footpath is that the cutting of 0.41～1.0cm is for cuttage。

Cutting soaks 8 hours in mixed solution。

In mid-February, take 1 year raw nursery stock branch。Reduce cold preservation time, improve activity, meanwhile, reduce cold preservation cost。

Beneficial effect: the technical program has filled up the technological gap of styrax tonkinensis Craib ex Hart cottage propagation, cuttage survival rate is high, can reach 70%, and cost is low, it is easy to operation, it may be achieved the high-volume breeding of styrax tonkinensis Craib ex Hart high-quality clone nursery stock。

Detailed description of the invention

Below in conjunction with Figure of description, technical scheme is described in further detail。

Embodiment one:

The technical program comprises the following steps:

Fringe bar gathers and processes: mid-February, gather the branch of more than aerial parts 0.2m, wipe out side shoot and only retain trunk, be then cut to 0.4～0.5m length, two ends mouth of sealing with wax depending on beam length before within 1 year, raw nursery stock sprouts；

Fringe bar cold preservation: wrap up the fringe bar sealed with wax with moistening absorbent cotton, be then placed in sealed plastic bag, be placed in 2～5 DEG C of low-temperature conservation cabinets are stored standby；

Cutting time: select fair weather to carry out mid-April；

Seedbed prepares: with 1m width, cement pit high for 25cm for cuttage seedbed, and in bed, paving is using perlite thick for 20cm as substrate, and 1m is that spacing takes Small plastic shed, uses covered rearing with plastic film。With the uniform spray disinfectant of potassium permanganate solution before cuttage；

Cutting prepares and processes: is taken out the fringe bar of storage, is cut into about length 10cm, cutting with more than 1 bud, diameter 0.2～0.4cm。Bud is positioned at the upper position of cutting, cutting top lateral shear, lower end oblique shear。Configuration mixed solution, the mass percent concentration of GGR6, IBA respectively 300mg L in mixed solution^-1、50mg·L^-1。It is one group by ready some cutting bundle, ties and be placed in the mixed solution prepared and soak 4 hours, soaking depth 4～5cm。First punching in substrate with the bamboo let more bigger than cutting rugosity before cuttage, the jack degree of depth 8～10cm, density is 10cm × 10cm。Putting into compacting after cutting, after having inserted, water spray is saturated to substrate, is then built by shed；Control measures: after cuttage, every day observes, and moisturizing of suitably spraying water, as opened ventilation and heat by shed two when temperature reaches 30 DEG C。Transplant after having more fibrous root to grow。

Embodiment two: exist together with embodiment one phase and repeat no more, different places are in that: the mass percent concentration of GGR6, IBA respectively 300mg L in mixed solution^-1、100mg·L^-1；Soak time in mixed liquor is 6 hours。

Embodiment three: exist together with embodiment one phase and repeat no more, different places are in that: the mass percent concentration of GGR6, IBA respectively 300mg L in mixed solution^-1、150mg·L^-1, the soak time in mixed liquor is 8 hours。

Embodiment four: exist together with embodiment one phase and repeat no more, different places are in that: selecting diameter is the cutting of 0.41～1.0cm, and cutting soak time in mixed liquor is 6 hours。

Embodiment five: exist together with embodiment one phase and repeat no more, different places are in that: selecting diameter is the cutting of 0.41～1.0cm, the mass percent concentration of GGR6, IBA respectively 300mg L in mixed solution^-1、100mg·L^-1；Soak time in mixed liquor is 8 hours。

Embodiment six: exist together with embodiment one phase and repeat no more, different places are in that: selecting diameter is the cutting of 0.41～1.0cm, the mass percent concentration of GGR6, IBA respectively 300mg L in mixed solution^-1、150mg·L^-1, the soak time in mixed liquor is 4 hours。

Embodiment seven: exist together with embodiment one phase and repeat no more, different places are in that: selecting the diameter cutting more than 1.0cm, cutting soak time in mixed liquor is 8 hours。

Embodiment eight: exist together with embodiment one phase and repeat no more, different places are in that: select the diameter cutting more than 1.0cm, the mass percent concentration of GGR6, IBA respectively 300mg L in mixed solution^-1、100mg·L^-1；Soak time in mixed liquor is 8 hours。

Embodiment nine: exist together with embodiment one phase and repeat no more, different places are in that: select the diameter cutting more than 1.0cm, the mass percent concentration of GGR6, IBA respectively 300mg L in mixed solution^-1、150mg·L^-1；Soak time in mixed liquor is 6 hours。

The Cutting test of embodiment 1 to 9 processes and interpretation of result is as shown in the table:

By upper table result it can be seen that when adopting embodiment 5, styrax tonkinensis Craib ex Hart cuttage survival rate has and significantly promotes, and its cuttage survival rate is up to more than 70%。

A kind of styrax tonkinensis Craib ex Hart cuttage breeding method is specific embodiments of the invention; embodied substantive distinguishing features of the present invention and progress, can according to actual use needs, under the enlightenment of the present invention; it is carried out the equivalent modifications of the aspect such as shape, structure, all at the row of the protection domain of this programme。

Claims

1. a styrax tonkinensis Craib ex Hart cuttage breeding method, it is characterised in that comprise the following steps:

4) cuttage cutting: mid-April, when mean temperature reaches more than 15 DEG C, takes out the fringe bar of storage, is cut into length 10cm～12cm, cutting with more than 1 bud；Bud is positioned at the upper position of cutting, cutting top lateral shear, lower end oblique shear；It is one group by some cutting, ties and be placed in the mixed solution containing GGR6, IBA prepared and soak 8 hours, the mass percent concentration of GGR6, IBA respectively 300mg L in mixed solution^-1、100mg·L^-1；Soaking depth 4～5cm；First punching in substrate with the bamboo let thicker than cutting before cuttage, the jack degree of depth 8～10cm, density is 10cm × more than 10cm；Cut-off footpath be the cutting of 0.41～1.0cm for cuttage, put into compacting substrate after cutting, after having inserted, water spray is saturated to substrate, is then built by shed；

2. a kind of styrax tonkinensis Craib ex Hart cuttage breeding method according to claim 1, it is characterised in that: in mid or late Febuary, take 1 year raw nursery stock branch。