CN106010550A - Soil improving agent - Google Patents

Soil improving agent Download PDF

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Publication number
CN106010550A
CN106010550A CN201610343160.5A CN201610343160A CN106010550A CN 106010550 A CN106010550 A CN 106010550A CN 201610343160 A CN201610343160 A CN 201610343160A CN 106010550 A CN106010550 A CN 106010550A
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China
Prior art keywords
soil
weight portions
ingredient
mycopowder
aqueous suspension
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CN201610343160.5A
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Chinese (zh)
Inventor
汤长富
谢林君
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Individual
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Priority to CN201610343160.5A priority Critical patent/CN106010550A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/32Yeast
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00Agricultural use

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Pest Control & Pesticides (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Soil Sciences (AREA)
  • Plant Pathology (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Materials Engineering (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)
  • Fertilizers (AREA)

Abstract

The invention relates to a soil improving agent, which consists of an aqueous suspension ingredient and a microbe ingredient, wherein the aqueous suspension ingredient contains montmorillonoid and activated clay; the microbe ingredient consists of bacteria powder of bacillus licheniformis, bacillus subtilis, pichia pastoris, chromafium okenii and streptomyces microflavus. In a short time before the use, the aqueous suspension ingredient is mixed with the microbe ingredient; in the obtained suspension, the living bacteria count of each kind of bacteria is more than or equal to 1.00*10<9>/ml. The soil improving agent has good fertilizer maintenance capability on planting land, so that the soil can still maintain the identical or higher contents of most nutrition elements after the planting. In the next round of planting, the land subjected to application of the soil improving agent has the obviously higher basic fertility, so that the application of chemical fertilizers is reduced. In addition, in the soil subjected to the application of the soil improving agent, the total quantity of organic matters and microbes is obviously increased; the soil quality is improved; the soil hardening is prevented; the sustainable development of the soil is facilitated.

Description

A kind of soil conditioner
Technical field
The present invention relates to soil improvement field, more specifically it relates to a kind of soil conditioner.
Background technology
China is large agricultural country, 2,000,000,000 mu of existing arable land, and year, with chemical fertilizer 1.5 hundred million tons, is chemical fertilizer importer the biggest in the world With nitrogen fertilizer production state.But, in terms of the utilization rate of chemical fertilizer, however it remains the space of raising.Department's measuring and calculating according to the study, the most entirely The average utilization rate of nitrogen fertilizer in state various places is 30-35%, phosphate fertilizer 20-30%, potash fertilizer 30-50%.According to estimates, the whole nation is every year because blindly making With waste chemical fertilizer about 1,000,000 tons, cause huge economic loss.
Additionally, after experiencing repeatedly crop cycle, the nutrient that in soil, available plant growth is required is depleted.Agriculture The people often use chemical fertilizer to increase fertility to soil, and owing to using chemical fertilizer of simple substance in a large number for a long time, microorganism in soil and organic Matter is consumed, soil compaction, and loamy texture deteriorates.
Accordingly, it would be desirable to a kind of soil conditioner improves the content of microorganisms in soil, improves the soil conditioner of loamy texture, with Improve water conservation and the fertilizer-preserving ability of soil.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of soil conditioner, its by aqueous suspension component and Microbial components forms, and wherein said aqueous suspension component comprises montmorillonite and active hargil, and described microbial components is by ground Clothing bacillus cereus (Bacillus licheniformis), bacillus subtilis (Bacillus subtilis), Pichia sp. (Pichia pastoris), Ovshinsky chomophoric bacterium (Chromatium okenii), streptomyces microflavus (Streptomyces Microflavus) mycopowder composition, immediately before use, mixes described aqueous suspension component with described microbial components, In the suspensoid obtained, in described Bacillus licheniformis, bacillus subtilis, Pichia sp., Ovshinsky chomophoric bacterium, streptomyces microflavus Each of living bacteria count >=1.00 × 109/ml。
Further, described aqueous suspension component is by the water of 70 weight portions, the montmorillonite of 7 weight portions and 6 weight portions Active hargil forms.
Further, described microbial components is by the Bacillus licheniformis powder of 4 weight portions, the hay spore of 2 weight portions Bacillus mycopowder, the Pichia sp. mycopowder of 2 weight portions, the Ovshinsky chomophoric bacterium mycopowder of 3 weight portions, the streptomyces microflavus bacterium of 2 weight portions Powder forms.
The using of montmorillonite and active hargil can increase the quantity of soil agreegate, reduce the soil weight, increase soil Earth porosity, had both improved the water retention property of soil, reduces and oozes under moisture, save irrigation water, reduces and water man-hour, and prolongation is poured water Interval, reduces soil erosion.Additionally, montmorillonite also provides attaching substratum for the existence of microorganism and breeding, make microorganism not To external diffusion.
Organic matter in bacillus subtilis and Bacillus licheniformis decomposable asymmetric choice net soil, discharges a large amount of enzyme, aminoacid, and CO is also discharged in metabolic process2Promote that the root system of plant is to fertilizer and the absorption of inorganic ions.
Streptomyces microflavus can secrete the important Producer of some row bioactive substances such as antibiotic, at diseases and pests of agronomic crop Biological control aspect has important function.Can be with synthetic starch enzyme, cellulase, aminoacid urgency vitamin etc. is multiple useful Metabolite.
Pichia sp. is important nutrient functional antibacterial, can be effective to including in the various matrix environment such as soil, water body Nutrient reasonably converts and high efficiency absorption;Also ferment decomposition, growth metabolism can be formed multiple B race dimension Raw element and biological active substances, strengthen the activity of above microorganism.
Ovshinsky chomophoric bacterium can carry out photosynthesis, for providing organic matter in dead soil, and provides for above microorganism Portion of energy and material source, and the metabolism of the oxygen promotion mentioned microorganism discharged.
Present invention also offers a kind of method improveing soil, be applied to described soil including by above-mentioned soil conditioner Step.
Further, said method comprising the steps of:
1) with water by described soil improvement dilution agent 100 times;
2) soil conditioner of dilution is watered in described soil with the amount of every square metre of 500ml.
Detailed description of the invention
Being described principle and the feature of the present invention below in conjunction with embodiment, example is served only for explaining the present invention, It is not intended to limit the scope of the present invention.
1. the formula of the soil conditioner of the present invention
Aqueous suspension component:
Montmorillonite 700g
Active hargil 600g
Water 7kg
Microbial components:
Wherein, above-mentioned mycopowder obtains by the following method: Bacillus licheniformis, bacillus subtilis, Pichia sp. are divided Be not inoculated in equipped with in the 200L fermentation tank of LB fluid medium, Ovshinsky chomophoric bacterium is inoculated in equipped with by 1000 weight parts waters, 5 Weight portion yeast extract, 3 parts by weight acetic acid sodium, 0.5 weight portion ammonia chloride, 0.5 parts by weight of phosphoric acid potassium dihydrogen and 0.3 parts sulfuric acid In the 200L fermentation tank of the culture medium of magnesium composition, streptomyces microflavus is inoculated in and synthesizes a culture medium 200L fermentation equipped with Gao Shi In tank, cultivate 30 hours in 37 DEG C, cultivate and obtain five kinds of cultures, use the cell in each culture of determination of cell count Number, centrifugal, abandon supernatant, collect bacterium mud.
2. the use example of soil conditioner
This example is biological by assembled a crowd power group Cephalanthera falcata (Thunb) Lindl moral of Inst of Economical Crops breeding and Cultivation, Sichuan Academy of Agricultur Science and Technology Ltd. entrusts and performs, and uses the soil conditioner of the present invention, to observe its effect on Semen arachidis hypogaeae.
1. experimental plot overview
This test is carried out through work proving ground, bluish white river in academy of agricultural sciences of Sichuan Province, and soil is the flat field of mortise, and soil property earth is inclined Mud, fertility is preferable.Trial zone Semen arachidis hypogaeae strain is 1503, and isolation area strain is colored Semen arachidis hypogaeae C5.On April 28th, 2015 is sowed.
Using ridge planting, random district group arranges, and community is wide 2.4 meters, long 8 meters, plot area 19.2 square metres, wide-and narrow-row (0.5 and 0.3 meter), nest away from 0.20 meter, 6 row districts, often row 40 nest, every community 240 nest, every nest stays double strain, density be 8333 nests/ Mu.Field uses the group arrangement of random district, repeats for 3 times, Gong36Ge community.In the soil activation agent of the present invention used by test Comprising live organism, meeting automatic moving in soil, for the accuracy tested, field each two arranges a sky between processing Bai little Qu plants other Semen arachidis hypogaeae materials and makees to isolate, blank totally 33, community, aisle 70cm between repetition, and minizone does not set aisle, and four Protection row is set in week, tests floor space about 2.0 mu.
2. soil treatment
Base fertilizer Sa Kefu compound fertilizer, purchased from China-Arab Fertilizer Co., Ltd (English abbreviation SACF), mainly contains Amount is N-P2O5-K2O (16:6:18), application process is for spreading fertilizer over the fields solid fertilizer.The application process of the soil conditioner of the present invention is: incite somebody to action this The soil conditioner of invention loads in the container of spraying liquid, dilute with water 100 times, by the liquid after dilution according to every square metre The consumption of 500 milliliters is sprayed in soil uniformly.In the trial zone using this bacterial manure, within 15 days, any killing can not be used Microbial inoculum or insecticide.Each process is as shown in table 1:
Table 1. soil treatment
3. treatment effect
Carrying out 3 peanut growth condition surveys in peanut growth phase, florescence, lower pin fruiting period, main investigation Semen arachidis hypogaeae is raw The projects such as growing way, regularity, incidence, carry out induction-arrangement by survey data.Result shows, from the point of view of variable rate technology, and 5 The peanut growth situation of process process group is all preferable, and morbidity is few, and cell portion is due to cultivation reason, and weeds are more, and growth is subject to Certain impact.
Table 2 shows the economic benefit statistics of these five process groups.
The economic benefit of table 2. each process group
From the point of view of the output value and economic benefit, process 1 (Sa Kefu 37.5), process 3 (Sa Kefu 37.5, soil conditioners 3.5) performance is the most similar.Process mixed with soil conditioner of Sa Kefu shows preferably on peanut production, soil conditioner Should not be administered alone, a certain amount of chemical fertilizer or fertilizer need to be coordinated to be used together.
Carry out 3 soil testings work in the peanut growth phase, and the microorganism in soil is counted, analyze soil and change The good dose of impact on soil microorganism, uses the soil test instrument of Sol company of the U.S. to survey soil trace element simultaneously Examination.Table 3 shows processing 3 (Sa Kefu 37.5, soil conditioners 3.5) and processing three surveys that 1 (Sa Kefu 37.5) is carried out The overview of soil microorganism, main nutrient elements and organic matter that examination obtains, table 4 shows processing 3 (Sa Kefu 37.5, soil Soil improver 3.5) and process the overview of the soil trace element that twice test carrying out of 1 (Sa Kefu 37.5) obtains.
Microorganism in table 3. soil and the overview of main nutrient elements
The overview of the trace element in table 4. soil
Be can be seen that by table 3 and table 4, when the testing time is for sowing, after the lower pin phase of blooming has gathered in the crops, owing to July is In the period that peanut growth is the most vigorous, nutrients all kinds of to soil consume relatively big, the therefore test in July, tri-kinds of elements of N, P, K All occur in that bigger decline.As can be seen from the table, employ the soil of the soil conditioner of the present invention, its nitrogen, phosphorus, potassium etc. The content of main nutrient elements is above blank, wherein, has declined, reduce by 13.2% before and after nitrogen content plantation, and empty White comparison reduces 33.8%;Potassium content is basicly stable before and after planting, and adds 3.4%, and blank reduces 13.0%; Test phosphorus content occurs in that bigger amplification, adds 28%, and blank reduces 8.1%, it can be said that open-birth thing Bacterial manure effectively N, P, K content in improving soil has bigger potentiality, can effectively reduce the forfeiture of fertility in soil, it is achieved Gu fertile The purpose of volume increase.Adding 10.2% before and after content of organic matter plantation in testing soil, microbial count increases by 33.3%, and empty White comparison organic matter have dropped 13.3%, and microbial count reduces 11.1%, illustrates that in bio-bacterial manure, antibacterial has stronger Vitality, the growth to promotion Soil Microorganism, rich soil, the content adding soil with organic matter has relatively your writing With.Owing to the cost of trace elements determination is relatively big, the most only measures twice, application of the soil of the soil conditioner of the present invention In, in trace element, ferrum, manganese, sulfur content occur in that bigger lifting, and each trace element of blank all occurs in that different journey The decline of degree.Declining occur in Cr, Ca, V, and wherein the range of decrease of Cr and Ca is similar with blank, and the reduction of V is right less than blank According to.These results indicate that the soil conditioner of the present invention has significant fertilizer-preserving ability to plantation land used, effectively reduce plantation The fertility of land used is lost, and even can improve the content of some nutrients.
By result above, the soil conditioner of the present invention has good fertilizer-preserving ability to plantation land used so that soil Earth can after planting major part nutrient remain in that with plant front same or higher.Although First Year is at volume increase and chemical fertilizer Minimizing aspect has no positive effect, however, it is possible to it is envisioned that in the plantation of next round, used the soil conditioner of the present invention Land used have significantly higher basic fertility than the land used of the soil conditioner of not using the present invention, therefore on fertilizer amount Thus it is minimized, thus reduces chemical fertilizer application.Additionally, application of in the soil of the soil conditioner of the present invention, organic and Microbial count significantly raises, and improves loamy texture, keeps soil from packing together, beneficially the sustainable development of soil.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and Within principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.

Claims (5)

1. a soil conditioner, it is characterised in that be made up of aqueous suspension component and microbial components, wherein said aqueous Suspensoid component comprises montmorillonite and active hargil, and described microbial components is by Bacillus licheniformis, bacillus subtilis, Bi Chi Yeast, Ovshinsky chomophoric bacterium, the mycopowder composition of streptomyces microflavus, immediately before use, by described aqueous suspension component with described Microbial components mixes, in the suspensoid obtained, and described Bacillus licheniformis, bacillus subtilis, Pichia sp., Ovshinsky coloring Living bacteria count >=1.00 × 10 each of in bacterium, streptomyces microflavus9/mL。
Soil conditioner the most according to claim 1, it is characterised in that described aqueous suspension component is by 70 weight portions The active hargil composition of water, the montmorillonite of 7 weight portions and 6 weight portions.
Soil conditioner the most according to claim 1, it is characterised in that described microbial components is by the lichens of 4 weight portions Bacillus cereus mycopowder, the bacillus subtilis mycopowder of 2 weight portions, the Pichia sp. mycopowder of 2 weight portions, the Ovshinsky of 3 weight portions Color bacterium mycopowder, the streptomyces microflavus mycopowder composition of 2 weight portions.
4. the method improveing soil, it is characterised in that include the soil conditioner described in claim 1-3 is applied to institute State the step of soil.
Method the most according to claim 4, it is characterised in that comprise the following steps:
1) with water by described soil improvement dilution agent 100 times;
2) soil conditioner of dilution is watered in described soil with the amount of every square metre of 500ml.
CN201610343160.5A 2016-05-20 2016-05-20 Soil improving agent Pending CN106010550A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108293345A (en) * 2017-01-12 2018-07-20 喜施倍全球股份有限公司 Microbe soil enhances
CN108947673A (en) * 2018-08-14 2018-12-07 侯希波 A kind of soil improvement Natural Circulation microbial bacterial agent and preparation method thereof
EP3657950A4 (en) * 2017-07-27 2021-08-11 Locus Agriculture IP Company, LLC Efficient production of pichia yeasts and their use for enhancing plant and animal health
US11447430B2 (en) 2018-05-08 2022-09-20 Locus Agriculture Ip Company, Llc Microbe-based products for enhancing plant root and immune health

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Publication number Priority date Publication date Assignee Title
CN100448943C (en) * 2007-01-09 2009-01-07 山东省农业科学院土壤肥料研究所 Vegetable field soil improving agent and its preparation process and application
KR100914763B1 (en) * 2008-11-24 2009-08-31 (주)메덱스에프씨 Quality of soil improvement material and manufacturing method for the same
CN101760202A (en) * 2008-12-25 2010-06-30 上海拜森生物技术有限公司 Compound biological soil improver and preparation method thereof
CN102061176A (en) * 2011-01-06 2011-05-18 四川大学 Biologic modifier special for side slope artificial soil
CN102656971A (en) * 2012-04-13 2012-09-12 郝国成 Production method and application of sandy land conditioner made from bentonite
CN102766464A (en) * 2012-07-05 2012-11-07 昆明豪原特自控有限公司 Biological soil conditioner and preparation method thereof
CN102876576A (en) * 2012-09-24 2013-01-16 湖南泰谷生物科技有限责任公司 Acidic soil conditioner, and preparation method and application thereof
CN103468267A (en) * 2013-09-18 2013-12-25 南阳市福来石油化学有限公司 Composite mineral soil repair agent
CN104498040A (en) * 2014-11-10 2015-04-08 杨凌绿都生物科技有限公司 Application of Bacillus amyloliquefaciens as soil conditioner
CN104629766A (en) * 2014-12-16 2015-05-20 镇江拜因诺生物科技有限公司 Microorganism soil remediation agent

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100448943C (en) * 2007-01-09 2009-01-07 山东省农业科学院土壤肥料研究所 Vegetable field soil improving agent and its preparation process and application
KR100914763B1 (en) * 2008-11-24 2009-08-31 (주)메덱스에프씨 Quality of soil improvement material and manufacturing method for the same
CN101760202A (en) * 2008-12-25 2010-06-30 上海拜森生物技术有限公司 Compound biological soil improver and preparation method thereof
CN102061176A (en) * 2011-01-06 2011-05-18 四川大学 Biologic modifier special for side slope artificial soil
CN102656971A (en) * 2012-04-13 2012-09-12 郝国成 Production method and application of sandy land conditioner made from bentonite
CN102766464A (en) * 2012-07-05 2012-11-07 昆明豪原特自控有限公司 Biological soil conditioner and preparation method thereof
CN102876576A (en) * 2012-09-24 2013-01-16 湖南泰谷生物科技有限责任公司 Acidic soil conditioner, and preparation method and application thereof
CN103468267A (en) * 2013-09-18 2013-12-25 南阳市福来石油化学有限公司 Composite mineral soil repair agent
CN104498040A (en) * 2014-11-10 2015-04-08 杨凌绿都生物科技有限公司 Application of Bacillus amyloliquefaciens as soil conditioner
CN104629766A (en) * 2014-12-16 2015-05-20 镇江拜因诺生物科技有限公司 Microorganism soil remediation agent

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108293345A (en) * 2017-01-12 2018-07-20 喜施倍全球股份有限公司 Microbe soil enhances
EP3657950A4 (en) * 2017-07-27 2021-08-11 Locus Agriculture IP Company, LLC Efficient production of pichia yeasts and their use for enhancing plant and animal health
US11324224B2 (en) 2017-07-27 2022-05-10 Locus Agriculture Ip Company, Llc Efficient production of Pichia yeasts and their use for enhancing plant and animal health
IL271792B (en) * 2017-07-27 2022-12-01 Locus Agriculture Ip Co Llc Efficient production of pichia yeasts and their use for enhancing plant and animal health
TWI796345B (en) * 2017-07-27 2023-03-21 美商路卡斯農業智慧財產有限責任公司 Efficient production of pichia yeasts and their use for enhancing plant and animal health
IL271792B2 (en) * 2017-07-27 2023-04-01 Locus Agriculture Ip Co Llc Efficient production of pichia yeasts and their use for enhancing plant and animal health
US11447430B2 (en) 2018-05-08 2022-09-20 Locus Agriculture Ip Company, Llc Microbe-based products for enhancing plant root and immune health
CN108947673A (en) * 2018-08-14 2018-12-07 侯希波 A kind of soil improvement Natural Circulation microbial bacterial agent and preparation method thereof

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Application publication date: 20161012