CN106831233A - Biological organic soil that a kind of utilization compost product improvement foundation pit earth is obtained and preparation method thereof - Google Patents
Biological organic soil that a kind of utilization compost product improvement foundation pit earth is obtained and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
Biological organic soil obtained the invention provides a kind of utilization compost product improvement foundation pit earth and preparation method thereof.The present invention obtains biological organic soil by after the compost product that pig manure and garden waste are produced according to certain initial carbon-nitrogen ratio fermentation maturity and foundation pit earth mixing, inoculating into the strain with lignocellulose degradation.The present invention weighs improved effect by determining physical arrangement, chemical index, biological indicator etc. of biological organic soil, the formation of urban construction roach crumb structure is promoted to a certain extent and improves original barren nutrient and microbial biomass in urban construction roach, for the recycling of garden waste and urban construction roach provides a kind of practicable method, while proposing the concept of biological organic soil on the basis of native standard is transplanted in afforestation.
Description
Technical field
The invention belongs to urban construction roach and garden waste comprehensive utilization of resources field of engineering technology, and in particular to
Biological organic soil that a kind of utilization compost product improvement foundation pit earth is obtained and preparation method thereof.
Background technology
With the quickening of China's urban afforestation process, urban afforestation is increasing to the demand of soil, is fetched earth meeting from farmland
Destruction Agro-ecological Structure, constitutes a threat to normal agricultural production, produces substantial amounts of city to build in another aspect urban construction
If roach, itself nutrient content of these urban construction roach is low, and moisture holding capacity is low, arbitrarily topples over and can cause soil erosion, dirty
Dye environment, what urban construction roach had is used to be backfilling into greenbelt, easily causes plant growth malnutritive, and this is mainly city
City's construction roach porosity is few, and the content of organic matter is low, meets water easily flood stain, and chance is non-irrigated easily dry, and various nutriments are deficient, cause
Plant root growth is poor, and urban construction roach nutrition causes Soil Microorganism structure of community diversity while barren
Low, various effective bacterium activity are low, and various greenbelts and ornamental plant residuum the like waste cannot degrade for a long time after dropping to ground.
The content of the invention
It is an object of the invention to provide a kind of utilization compost product improvement foundation pit earth obtain biological organic soil and its
Preparation method, the present invention is main to utilize pig manure and garden waste（Deadwood leaf）According to initial carbon-nitrogen ratio 25:1-30:1 mixing
Afterwards, regulation water content is fermented to 60%-65%, is added in batches after the megathermal period a certain amount of with lignocellulose degradation
Strain.Mix by a certain percentage according to composting production and urban construction roach after compost fermentation maturation, and be inoculated with the micro- life of function
Thing strain, obtains biological organic soil.The biological organic soil organic matter content for obtaining enriches, and nutrient is comprehensively, various micro-
Secondary element is balanced, and unit weight is small, and water retention property is good, and porosity is big, and microbe colony is rational in infrastructure, lignocellulose degradation ability
By force, the formation for lasting maintenance soil organism high content and promotion crumb structure soil has great significance.
For achieving the above object, the present invention is achieved using following technical scheme：
The preparation method of the biological organic soil obtained the invention provides a kind of utilization compost product improvement foundation pit earth, the system
Preparation Method is comprised the following steps：
（1）The preparation of fermentation materials：By pig manure and garden waste according to C/N=25:1~30:1 well mixed obtains fermentate
Material, controls the water content of fermentation materials in 60%-65%, starts compost aerobic fermentation, keeps the megathermal period fermentation of 11-14 days；
（2）Subsequently into cooldown period, compost carry out within the 12-15 days it is inoculum fermented for the first time, access the flat lead fungi of yellow archespore enzyme,
Trametes versicolor and green spore streptomycete；
（3）Second inoculum fermented, access Coriolus Versicolor, aspergillus niger and trichoderma reesei are carried out within the 20-22 days in compost；Fermentation
To the 38-41 days of compost, the compost product that acquisition is become thoroughly decomposed；
（4）Biological organic soil processing：The compost product and foundation pit earth are compared into 0.4-0.5 according to weight:1 ratio is mixed
Close uniform, colloid bacillus cereus are sprayed to mixed soil surface, the biological organic soil is obtained after mixing.
Further：Megathermal period temperature described in composting fermentation process is 55 DEG C -70 DEG C, and cooldown period temperature is 40 DEG C -50
℃。
Further：The step（2）The density of middle strain is respectively：Green spore streptomycete is 0.8 × 1010CFU/1kg is wet
Material -1.5 × 1010CFU/1kg wet stocks, Phanerochaete chrysosporium is 0.8 × 1010CFU/1kg wet stocks -1.5 × 1010
CFU/1kg wet stocks, Trametes versicolor is 0.8 × 1010CFU/1kg wet stocks -1.5 × 1010CFU/1kg wet stocks.
Further：The step（3）The density of middle strain is respectively：Coriolus Versicolor is 0.8 × 1010The wet things of CFU/1kg
Material -1.5 × 1010CFU/1kg wet stocks, aspergillus niger is 0.8 × 1010CFU/1kg wet stocks -1.5 × 1010CFU/1kg is wet
Material, trichoderma reesei is 0.8 × 1010CFU/1kg wet stocks -1.5 × 1010CFU/1kg wet stocks.
Further：The step（4）The density of middle strain is：Colloid bacillus cereus 0.8 × 1010The wet samples of CFU/1kg
Soil -1.2 × 1010The wet sample soil of CFU/1kg.
Present invention also offers the biological organic soil that described preparation method is obtained.
Further：The biological organic soil weight is in 1.14-1.26Mg/m3。
Further：The biological organic P in soil H is 6.5-7.5.
Further：The biological organic soil organism is in >=30g/kg.
Further：The biological organic soil available phosphorus content reaches 10-50mg/kg, and quick-acting potassium content reaches 100-
250mg/kg, hydrolyzable nitrogen content reaches 150-200mg/kg.
Compared with prior art, advantages of the present invention and have the technical effect that：The original of the making compost product that the present invention is provided
Material wide material sources, composting process is simple to operate, easily manipulation, realizes agriculture and forestry organic waste material fast degradation, pig manure and agriculture and forestry organic waste material
Ferment together, especially after the garden waste abundant with the lignocellulose-containing such as wood chip fermentation, the part heavy metal in pig manure
Can be passivated, the biological effectiveness reduction of heavy metal, while pig manure and garden waste mixed fermentation and single pig manure fermentation phase
Reduce than nitrogen loss, be afterwards biological organic soil using the composting production improvement urban construction roach of production, realize city
Build roach（Foundation pit earth）Turn waste into wealth.
The preparation process of biological organic soil of the present invention is divided into two steps, is first compost fermentation, including water content debugging,
The control of temperature, ventilation（Oxygen content）Control, cooldown period inoculation lignocellulose degrading bacteria；Secondly, the composting production for becoming thoroughly decomposed
It is inoculated with according to a certain percentage with urban construction roach and corresponding function microorganism fungus kind, is held by determining biological organic soil
Weight, biological organic soil porosity, biological organic Cation Exchange Capacity in Soils, biological organic soil conductivity, biological organic soil
Earth organic matter, biological organic soil hydrolyzable nitrogen studies whether biological organic soil reaches standard.The method and other soil
Modification method compares the recycling for realizing garden waste and urban construction roach, while being vaccinated with wooden with degraded
The strain of cellulose,, to lasting improvement soil texture, the present invention is for improving soil organic matter content important in inhibiting for this.
Brief description of the drawings
Fig. 1 is the comparing result of the biological organic soil and urban construction roach (foundation pit earth) content of organic matter；
Fig. 2 is the comparing result in the biological organic soil and the nonactive space of foundation pit earth, hollow billet space and ventilation voidage；
Fig. 3 is the comparing result of the biological organic soil and foundation pit earth unit weight；
Fig. 4 is the biological organic soil and foundation pit earth cation exchange capacity (CEC)（CEC）Comparing result；
Fig. 5 is the comparing result of the biological organic soil and foundation pit earth soil conductivity；
Fig. 6 is water-disintegrable nitrogen content comparing result in the biological organic soil and foundation pit earth；
Fig. 7 is microorganism total amount and superior microorganism coenocorrelation result in the biological organic soil and foundation pit earth；
Fig. 8 is the comparing result of bracketplant bracketplant chlorophyll content under the biological organic soil and foundation pit earth planting conditions.
Specific embodiment
Technical scheme is further described in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
Utilization compost product improvement urban construction roach of the invention is that the preparation method of biological organic soil is comprised the following steps：
1st, the preparation of compost product
（1）By garden waste（Deadwood leaf）Material of the particle diameter in 0.1-5mm sizes is ground into, by determining fresh pig respectively
Nitrogen content, the phosphorus content of excrement and deadwood number leaf, according to C/N=25:1 is uniformly mixed so as to obtain fermentation materials, afterwards according to water content in fermentation
Setting, makes the water content of fermentation materials be maintained at 65% or so after moisturizing, compost fermentation is proceeded by, while every turning one in six days
It is secondary, it is ensured that compost is aerobic fermentation, carry out temperature analysis to compost fermentation, keep the megathermal period fermentation of 14 days, the 14th day from height
The warm phase slowly enters cooldown period.
（2）Into cooldown period, the strong strain of lignocellulose degradation ability, the strain of addition are added within the 15th day in compost
For：The yellow flat lead fungi of archespore enzyme（Purchase is numbered from Chinese industrial Microbiological Culture Collection administrative center：40299）, Trametes versicolor
（Purchase is numbered from Chinese industrial Microbiological Culture Collection administrative center：50001）, green spore streptomycete（Purchase is common micro- from China
Biological inoculum preservation administrative center, numbering：4.1770）, the density of strain is respectively：Green spore streptomycete（1×1010CFU）/1kg
Wet stock, Phanerochaete chrysosporium（1×1010CFU）/ 1kg wet stocks, Trametes versicolor（1×1010CFU）/ 1kg wet stocks,
Carry out plus bacterium fermentation.
（3）In the bacterium that connect for the second time for the 22nd day of compost, Coriolus Versicolor is accessed（Buy from Chinese agriculture microorganism fungus kind
Preservation administrative center, numbering：50435）, aspergillus niger（Purchase is numbered from Chinese industrial Microbiological Culture Collection administrative center：
2109）And trichoderma reesei（Purchase is numbered from Chinese industrial Microbiological Culture Collection administrative center：41494）, according to Coriolus Versicolor
（1×1010CFU）/ 1kg wet stocks, aspergillus niger（1×1010CFU）/ 1kg wet stocks, trichoderma reesei（1×1010CFU）/1kg
Wet stock carries out inoculum fermented.The 38th day fermented to compost, heap temperature drops to environment temperature, and compost becomes thoroughly decomposed substantially.
Regular turning or aeration are passed through oxygen in compost fermentation, are passed through amount of oxygen not significantly reduce heap temperature simultaneously
Compost can be allowed to be advisable according to aerobic fermentation again.
2nd, compost product and urban construction roach（Foundation pit earth）Mixing and microorganism inoculation
The underground soil that foundation pit earth is mainly in Process of Urban Development or takes out in architecture construction, these underground soil one
Aspect is interfered less by artificially, on the other hand because these soil property top layer vegetation sparses, or these soil are deeper, root system of plant
Typically be difficult to reach, act on less with root system of plant, thus its feature to be mainly nutrition barren, retain water and nutrients performance is poor, soil property
Densification, meets water and is easy to run off.
By the compost product and above-mentioned foundation pit earth of above-mentioned acquisition according to weight ratio be 3:7 mixed after soil property surface spray
Colloid bacillus cereus liquid is spilt, inoculum concentration is colloid bacillus cereus（Purchase is numbered from Guangdong Province's Culture Collection, GIM：
GIM1.16）（1×1010CFU）The wet sample soil of/1kg, obtains biological organic soil of the present invention after being mixed directly.
3rd, the biological organic soil and original foundation pit earth for obtaining the present invention are compared, and experimental result is as follows
Fig. 1 indicates the content of organic matter of foundation pit earth and biological organic soil, the biological organic soil organic matter content after improvement
31.2g/kg is reached, has larger improvement relative to the content of organic matter of the g/kg of foundation pit earth 13.2.
Fig. 2 indicates the space distribution situation of foundation pit earth and biological organic soil, and nonactive hole is more in foundation pit earth, reaches
To 11%, growth of the nonactive hole to plant is unfavorable, and root system of plant cannot permeate into obtain nutrient.And biology has
, 4%, capillary interstice is more, reaches 39% for the nonactive hole of machine soil, and this is conducive to soil water-reataining fertilizer-keeping, and plant absorption
Nutrient, while space of ventilating reaches 12%, this is conducive to the breathing of root system of plant.
Fig. 3 indicates foundation pit earth and biological organic soil weight situation, the small soil of unit weight, and the soil is porous, beneficial to root system
Stretch, while the biological organic soil weight is 0.73 or so, be conducive to plant growth to fix.
Fig. 4 indicates foundation pit earth and biological organic soil CEC, and the biological organic soil CEC of improvement reaches 15.6cmol/
Kg is stronger compared to foundation pit earth fertilizer-preserving ability.
Fig. 5 indicates the contrast of foundation pit earth and biological organic soil conductivity, and suitable soil conductivity can keep
The activity of microorganism, foundation pit earth one side nutrition is barren, and another aspect soil conductivity is not suitable for microbial activities, for biology
Organic soil, because microbe species are more, structure of community is complicated, while soil conductivity is in 0.9mScm-1Left and right, Ke Yiwei
Hold edaphon greater activity.
Fig. 6 indicates foundation pit earth and the water-disintegrable nitrogen content of biological organic soil, and nitrogen is a great number of elements of plant growth,
The fertility of soil is reflected to a certain extent, and the water-disintegrable nitrogen content of biological organic soil can fully expire in 189mg/kg or so
The nitrogen nutrition demand of sufficient plant growth.
Fig. 7 indicates foundation pit earth and biological organic edaphon species diversity（It is total with biological organic Soil Microorganism
It is unit one to measure）, total microbes biomass is less in foundation pit earth, and species is few, mainly some indigenous type microorganisms.And it is biological
Organic soil is typical bacteria type soil, and amonifying bacteria accounts for microorganism total amount 56% or so, while various points be inoculated with before
The microbe species of solution cellulose and lignin all have certain abundance, in addition to indigenous microorganism, one in biological organic soil
A little fermentative microorganism species are also very abundant, and structure of community is reasonable.Biological organic soil nutrient level can be reflected high.
Fig. 8 indicates bracketplant and is planted in two kinds of soil respectively, by after bimestrial growth, dividing in every kind of cultivating soil
Not Chou Qu same area measuring blade its chlorophyll content, the bracketplant transplanted in foundation pit earth is integrally more thin and weak, and leaf color is shallower, and
The bracketplant physiological performance in biological organic soil is planted for leaf color is dark green, Chlorophyll-a Content is in 0.25 mg/gFW, chlorophyll
In 1.11mg/gFW, chlorophyll total amount is 1.36mg/gFW to b contents.
The determination experiment of embodiment 2, compost product, biological organic soil and the various index parameters of bracketplant
First, the performance of the biological organic soil that the present invention is obtained using the test of potted plant pilot production.
Selecting the basically identical bracketplant seedling of upgrowth situation carries out potted plant, the soil sample dress basin that compost product and foundation pit earth are mixed
Afterwards, it is colloid bacillus cereus 10 according to inoculum concentration6The amount of the wet sample soil of cfu/g is during basin bowl-entering soil table sprays bacterium solution, plant growth
Soil moisture content is kept 30% or so.
The measure of the biological organic soil weight
After potted plant experiment starts 10 days, basin bowl-entering soil matter forms nature and builds knot state, starts to determine the soil weight.Assay method is
The measure core cutter method of the soil weight of LY/T 1215.
The measure of biological organic soil porosity
After potted plant experiment starts 10 days, basin bowl-entering soil matter forms nature and builds knot state, starts to determine soil porosity.Assay method is
The measure core cutter method of the soil porosities of LY/T 1215.
Biological organic Cation Exchange Capacity in Soils（CEC）Measure
Assay method is the measure of the forest soil cation exchange capacity (CEC)s of LY/T 1243, ammonium acetate exchange process.
The measure of biological organic soil conductivity
Assay method is the measure of the forest soil electrical conductivity of LY/T 1251, conductance method（Water and soil mass ratio 5:1）.
The measure of the biological organic soil organism
Assay method is the measure of the forest soil organic matters of LY/T 1237, potassium dichromate oxidation-Outside Heating Method.
The measure of biological organic soil hydrolyzable nitrogen
Assay method is the measure of the forest soil hydrolyzable nitrogens of LY/T 1229, alkaline hydrolysis-diffusion method.
Biological organic soil total biomass and biocenological measure
Pot experiment is determined after starting 30 days.Assay method is high-flux sequence method.
The measure of biological organic soil potted plant chlorophyll content
Potted plant experiment is determined after starting 60 days.0.5g materials are weighed, the filament that width is 1 mm is cut into；Above-mentioned material is put into 10
In mL tool plug test tubes, directly with dark place soak extraction one week or so at 4 DEG C of 10 mL80% acetone, to blade is colourless or white, so
Chlorophyll solution is determined afterwards in 663 nm, the absorbance of 645 nm.
Calculate：Ca (mg/L)=12.71A663-2.59A645；Cb (mg/L)=22.88A645-4.67A663 CT=Ca+ Cb。
2nd, by analysis result：It is as follows using the criterion that compost product improvement foundation pit earth is biological organic soil：
2.1 compost fermentation material proportions：Result shows the carbon-nitrogen ratio 25-30 of design pig manure and garden waste during compost:1 is
Preferably, carbon-nitrogen ratio is too low, causes the loss of nitrogen serious, and when carbon-nitrogen ratio is too high, compost fermentation is slow, and heap temperature is low.According to microorganism
1 part of nitrogen of assimilation is needed to constitute own cells body during 5 parts of carbon of assimilation, because microorganism itself carbon-nitrogen ratio is 5:1, while assimilation
（Absorb）4 parts of organic carbons of consumption are needed to obtain energy during 1 part of carbon, so microorganism needs consumption profit when absorbing 1 part of nitrogen
With 25 parts of organic carbons.
Compost fermentation temperature：Continuing for composting fermentation process high temperature phase, helps to kill roundworm egg and various cause of diseases is micro-
Biology, it is catathermal persistently contribute to compost in hardly degraded organic substance further degrade, promote compost maturity, wherein of the invention
Megathermal period is advisable with 55 DEG C -70 DEG C, and the temperature more than 70 DEG C can all have undesirable effect for nutrition in compost and microorganism, this
The cooldown period of invention is advisable with 40 DEG C -50 DEG C, for the fungi of lignocellulose degradation provides suitable temperature.
The biological organic soil weight：Unit weight reflects the density of soil, is the consolidated statement of the soil texture and soil porosity
Existing, sandy soil space is thick, and total pore space amount is few, and unit weight is big, and clay space is small, and total pore space amount is more, and unit weight is small, the soil of crumb structure
Unit weight than hardened soil is small, and the excessive soil porosity that can react to a certain extent of unit weight is small, be unfavorable for root system of plant it is interspersed and
Breathing, the too small then soil of unit weight is excessively loose, is unfavorable for that root system of plant is fixed, so the unit weight of biological organic soil of the present invention exists
1.14-1.26Mg/m3Optimum plant growth.
Biological organic soil porosity：Soil particle in good soil（Solid phase）, soil moisture（Liquid phase）, soil air
（Gas phase）Ratio is 4 for plant and microorganism:3:3, liquid and gas all represent the content of soil void, what liquid phase was represented
It is to maintain the areolate content of capillary moisture, and it is have to promote the big of air circulation and drainage that gas phase is represented
The amount of hole, so during the noncapillary poropsity 12%-16% of biological organic soil of the present invention, capillary porosity is in 40%-
Optimum plant growth when 50%.
Biological organic Cation Exchange Capacity in Soils：The size of CEC, represent soil can retainable nutrient quantity, that is, protect
The height of fertilizer.The size of cation exchange capacity (CEC), can be used as the index for evaluating soil fertilizer ability.Cation exchange capacity (CEC) is soil
The main source of shock-absorbing capacity, is the important evidence of improved soil and the rational application of fertilizer, when cation exchange capacity (CEC) is high, soil fertilizer
Ability is strong, while can play cushioning effect to the chemical fertilizer of high concentration, it is to avoid plant is burnt.Biological organic soil of the present invention
The cation exchange capacity (CEC) of earth is >=15cmol (+)/kg.
Biological organic P in soil H：PH is limitation geobiont and chemically active threshold value, the activity of microorganism, nutriment
Absorption be all to be carried out under certain PH, peracid or cross alkali all can be to edaphon vital movement and cultivated plant
Root system nutrition absorbs and has an immense impact on, and wherein neutral soil is adapted to the growth of overwhelming majority plant, so life of the present invention
The PH of thing organic soil is advisable with 6.5-7.5.
Biological organic soil conductivity：Electrical conductivity is the threshold value for limiting plant and microbial activity, biology of the present invention
The conductivity range of organic soil is：0.1-1.20mS.cm-1。
The biological organic soil organism：The humus that organic matter is produced during being decomposed by the microorganisms promotes soil group
The formation of granulation, occurs in that big and small hole in soil, improve the aeration of soil, is arranged with space when soil moisture is excessive
Walk, while the small space for producing has water-retaining property.After organic matter is improved simultaneously, the species of the geobiont with organic matter as bait
All it is significantly increased with quantity, biology becomes variation, so that more organic matters are degraded to humus, promotes that soil is benign to follow
Ring, so the soil with organic matter of biological organic soil of the present invention is high-quality soil in >=30g/kg.
Biological organic soil available phosphorus：Available phosphorus in soil is the phosphorus component that can be absorbed by plants in soil, including complete
Portion's water-soluble phosphorus, partial adsorbates state phosphorus and organic phosphorus, soil available phosphorus are the indexs of soil phosphorus nutrient supply level height,
Soil phosphorus content height is reflecting the storage of Phosphorus in Soil and deliverability, biological organic of the present invention to a certain degree
When the available phosphorus content of soil reaches 10-50mg/kg, high-quality soil standard is reached.
Biological organic soil available nitrogen：The potassium being easily absorbed and used by plants, including exchangeable potassium and water-soluble potassium, the present invention
The quick-acting potassium content of the biological organic soil reaches 100-250mg/kg for high-quality soil.
Biological organic soil hydrolyzable nitrogen：Also referred to as soil validity nitrogen, it is ammonium nitrogen, nitrate nitrogen, amino acid, acid amides
It is effective part of soil nitrogen with the summation of the proteinic nitrogen of facile hydrolysis, can preferably reflects soil nitrogen at no distant date
Supply situation, occupies an important position on soil nitrogen level of supply is weighed, the hydrolysis of biological organic soil of the present invention
Property nitrogen content be high-quality soil when reaching 150-200mg/kg.
Chad content：Chad in soil can influence the activity of edaphon and the interspersed growth of root system of plant.When this
Chad particle diameter >=the 30mm for inventing the biological organic soil accounts for relatively good during total soil quality fraction≤5%.
The various content of beary metal of biological organic soil are as shown in table 1：
The standard that the various content of beary metal of table 1 should reach is（mg/kg）：
| PH value | Total cadmium≤ | Total mercury≤ | Total lead≤ | Total chromium≤ | Total arsenic≤ | Total nickel≤ | Total zinc≤ | Total copper≤ |
| ＜ 6.5 | 0.4 | 0.4 | 200 | 150 | 35 | 50 | 250 | 150 |
| ≥6.5 | 0.6 | 1.0 | 300 | 200 | 60 | 80 | 300 | 200 |
2.14 biological organic soil seed germination indexs：Soil seed germination index is that have unrestraint plant to determine in soil
The material of growth is present, and when the seed germination index of biological organic soil of the present invention is more than 80%, represents unrestraint in soil
The material of plant growth is present.
Biological organic edaphon total amount：Geobiont is that have vital major part in soil, wherein big absolutely
Part is microorganism, and the biomass of microorganism represents participation regulation and control soil energy and Nutrient Cycling and organic substance is converted into
The quantity of corresponding microorganism, closely related with the content of organic matter, Soil Microorganism generates various amino by growth
Acid, vitamin, enzyme etc. have nutritious material to plant, while stickum, excreta, remains of microorganism generation etc. turn into
The binding agent of soil particle, wherein fungi promote the pellet of soil, while Soil Microorganism is to the plant that is manured into soil
The forming process of the decomposition, biochemical cycles and soil texture of residuum and the soil organism and other hazardous compounds plays regulation
Effect, microbial total weight accounts for 0.1 ‰ of soil weight or so, and microbial total weight accounts for organic matter weight 1% or so.
Biological organic soil microbial community：Soil Microorganism composition is complicated, and wherein most belongs to active but not
Can cultivation type, the soil bacteria of 99.5%-99.9% or so cannot be separated in use for laboratory culture medium, edaphon it is many
Reflect to sample performance sensitive the quality of soil quality, microbiologic population's composition and bio-diversity of stabilization are high-quality soil
The necessary factor that earth should have, good soil is presented " bacteria type ", i.e., the soil with relatively low fungi and Bacteria percentage is more
Health.Bacterium accounts for 80% or so of quantities of soil microbe amount in rich soil, and colony density is 2 × 106Individual/g soil is big absolutely
Majority is heterotroph, and amonifying bacteria accounts for 70% of bacteria total amount or so, and colony density is 1.5 × 106Individual/g soil is other thin
Strain class is mainly nitrobacteria, SRB etc., wherein color bacillus, bacillus, mycobacteria, mould, aspergillus
Grade mould with clump is indigenous mircoflora, and some zymogenous microfloras are changed more sensitive by the soil organism, such as without bud
Spore bacillus, strepto- category, head mold, aspergillus, trichoderma, reaping hook are mould etc..The bacterium of harmful species is due to by fungi dominant bacteria such as wood
The antagonism of mould category and content is relatively low；Occupy the major class of edaphon second is actinomycetes, accounts for quantities of soil microbe amount
6%~15% or so, dominant bacteria is mainly some kinds of streptomycete, and for density, fungi density exists fungi in general soil
104~105, because its mycelium is wide, the total biomass of fungi is few unlike actinomyces, and species is mainly algal fungi, sac fungus,
Basidiomycetes and some Fungi Imperfectis, Fungi Imperfecti in fungi quantity at most, other kinds of microorganism such as some algae and primary
Animal occupies less.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than is limited；Although with reference to foregoing reality
Example is applied to be described in detail the present invention, for the person of ordinary skill of the art, still can be to foregoing implementation
Technical scheme described in example is modified, or carries out equivalent to which part technical characteristic；And these are changed or replace
Change, do not make the spirit and scope of the essence disengaging claimed technical solution of the invention of appropriate technical solution.
Claims (10)
1. a kind of utilization compost product improves the preparation method of the biological organic soil that foundation pit earth is obtained, it is characterised in that described
Preparation method is comprised the following steps：
（1）The preparation of fermentation materials：By pig manure and garden waste according to C/N=25:1~30:1 well mixed obtains fermentate
Material, controls the water content of fermentation materials in 60%-65%, starts compost aerobic fermentation, keeps the megathermal period fermentation of 11-14 days；
（2）Subsequently into cooldown period, compost carry out within the 12-15 days it is inoculum fermented for the first time, access the flat lead fungi of yellow archespore enzyme,
Trametes versicolor and green spore streptomycete；
（3）Second inoculum fermented, access Coriolus Versicolor, aspergillus niger and trichoderma reesei are carried out within the 20-22 days in compost；Fermentation
To the 38-41 days of compost, the compost product that acquisition is become thoroughly decomposed；
（4）Biological organic soil processing：The compost product and foundation pit earth are compared into 0.4-0.5 according to weight:1 ratio is mixed
Close uniform, colloid bacillus cereus are sprayed to mixed soil surface, the biological organic soil is obtained after mixing.
2. utilization compost product according to claim 1 improves the preparation method of the biological organic soil that foundation pit earth is obtained,
It is characterized in that：Megathermal period temperature described in composting fermentation process is 55 DEG C -70 DEG C, and cooldown period temperature is 40 DEG C -50 DEG C.
3. utilization compost product according to claim 1 improves the preparation method of the biological organic soil that foundation pit earth is obtained,
It is characterized in that：The step（2）The density of middle strain is respectively：Green spore streptomycete is 0.8 × 1010CFU/1kg wet stocks-
1.5×1010CFU/1kg wet stocks, Phanerochaete chrysosporium is 0.8 × 1010CFU/1kg wet stocks -1.5 × 1010 CFU/
1kg wet stocks, Trametes versicolor is 0.8 × 1010CFU/1kg wet stocks -1.5 × 1010CFU/1kg wet stocks.
4. utilization compost product according to claim 1 improves the preparation method of the biological organic soil that foundation pit earth is obtained,
It is characterized in that：The step（3）The density of middle strain is respectively：Coriolus Versicolor is 0.8 × 1010CFU/1kg wet stocks -1.5
×1010CFU/1kg wet stocks, aspergillus niger is 0.8 × 1010CFU/1kg wet stocks -1.5 × 1010CFU/1kg wet stocks, it is inner
Family name's trichoderma is 0.8 × 1010CFU/1kg wet stocks -1.5 × 1010CFU/1kg wet stocks.
5. utilization compost product according to claim 1 improves the preparation method of the biological organic soil that foundation pit earth is obtained,
It is characterized in that：The step（4）The density of middle strain is：Colloid bacillus cereus 0.8 × 1010The wet sample soil -1.2 of CFU/1kg ×
1010The wet sample soil of CFU/1kg.
6. the biological organic soil that the preparation method described in any one of claim 1-5 is obtained.
7. biological organic soil according to claim 6, it is characterised in that：The biological organic soil weight is in 1.14-
1.26Mg/m3。
8. biological organic soil according to claim 6, it is characterised in that：The biological organic P in soil H is 6.5-7.5.
9. biological organic soil according to claim 6, it is characterised in that：The biological organic soil organism >=
30g/kg。
10. biological organic soil according to claim 6, it is characterised in that：The biological organic soil available phosphorus content
10-50mg/kg is reached, quick-acting potassium content reaches 100-250mg/kg, and hydrolyzable nitrogen content reaches 150-200mg/kg.
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