CN102523787B - Culture method for increasing number and activity of alfalfa root nodules - Google Patents
Culture method for increasing number and activity of alfalfa root nodules Download PDFInfo
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- CN102523787B CN102523787B CN201210024784.2A CN201210024784A CN102523787B CN 102523787 B CN102523787 B CN 102523787B CN 201210024784 A CN201210024784 A CN 201210024784A CN 102523787 B CN102523787 B CN 102523787B
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- alfalfa
- nutrient solution
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses a culture method for increasing the number and activity of alfalfa root nodules. The culture method includes the following steps: after being disinfected, alfalfa seeds are placed into a culture dish with a piece of filter paper, and are germinated for 3 to 4 days in an illuminating incubator, the seedlings are then transplanted into a culture bowl filled up with quartz sand, rhizobia are inoculated during transplantation, nutrient solution is poured in the culture bowl, rhizobia are inoculated once again when the seedlings are grown to about 10cm, the nitrogen concentration of the nutrient solution is then decreased to less than 5mM, the nutrient solution is poured for one day, and water is poured for another day. The culture method can greatly increase the activity of alfalfa root nodules and the number of rhizobia and sufficiently exert the effect of root nodules in the growth of alfalfa, thus increasing the yield of alfalfa and improving the quality of alfalfa.
Description
Technical field
The invention belongs to agriculture field, in particular to a kind of alfalfa Phylloxera quantity and active cultural method of improving.
Background technology
Alfalfa (Medicago sativa L.) has another name called clover, lucerne, have the laudatory title of " King of Pasture ", be to cultivate in the world the earliest, distribute the widest, and be also the best, most important a kind of high-quality legume forage.Alfalfa is at existing more than 2000 cultivation history of China, and there is plantation all parts of the country, but are mainly distributed in northwest and North China.The hat of in the majority kind of leguminous forage of alfalfa hay output, and nutrient component is complete, is rich in protein, trace element and multivitamin, has the potentiality that are developed to health products, food additives and biological agent raw materials for production.Owing to thering is flourishing root system and the characteristic of biological nitrogen fixation, alfalfa or a kind of well soil melioration and water and soil conservation plant, establishing in large scale can improve soil nutrient, prevent the salinization of soil and desertification.The activity of rhizobium and how many production on clover have very important impact.At present, conventional alfalfa Phylloxera cultural method is earth culture, and this kind of method can be turned out root nodule, but the root nodule dross number of cultivating is few, active low, thereby has reduced the advantage of root nodule in alfalfa growing, has affected the yield and quality of clover thereupon.
Summary of the invention
The present invention seeks to the activity in order to improve alfalfa Phylloxera, increase rhizobium quantity, give full play to the effect of root nodule in alfalfa growing, thereby increase alfalfa output, improve alfalfa quality.In order to realize object of the present invention, intend adopting following technical scheme:
One aspect of the present invention relates to a kind of alfalfa Phylloxera quantity and active cultural method of improving, it comprises the steps: after alfalfa seed sterilization, be placed in the culture dish that is covered with filter paper, in illumination box, germinate, after germinateing 3-4 days, transplant to filling up in the culturing pot of quartz sand, when transplanting, carry out the inoculation of rhizobium, water with nutrient solution, growth of seedling carries out a legume inoculation during to 10cm left and right again, then, reduce the nitrogen concentration of nutrient solution to 5mM, carry out the alternately property pouring of one day nutrient solution one sky and water, described nutrient solution is formed by major constituents and microcomponent configuration, described major constituents comprises Ca (NO
3)
2, KCl, KH
2pO
4, KNO
3, K
2sO
4, MgSO
4and NH
4h
2pO
4, described microcomponent comprises H
3bO
3, CuSO
4, MnSO
4, (NH
4)
6mo
7o
24, ZnSO
4, FeSO
4and EDTA.
In a preferred embodiment of the present invention, the proportioning of described major constituents is 4723-5723: 956-1256: 676-976: 1516-2016: 1233-1533: 4314-5314: 1437-2037.
In a preferred embodiment of the present invention, the proportioning of described microcomponent is 4.24-6.24: 0.20-0.40: 3.80-5.80: 0.43-0.63: 0.49-0.70: 5570-7570: 7450-9450.
The present invention can increase substantially the activity of alfalfa Phylloxera, increases rhizobium quantity, gives full play to the effect of root nodule in alfalfa growing, thereby increases alfalfa output, improves alfalfa quality.
Embodiment:
In order to further illustrate technical scheme of the present invention, adopt following concrete scheme to cultivate alfalfa Phylloxera, it comprises the steps:
By alfalfa seed sterilization, (5% clorox soaks 5 minutes, 70% alcohol-pickled 10 seconds) after, be placed in the culture dish (100 seeds of each culture dish) that is covered with filter paper, in illumination box, (25 ℃ of light, dark 25 ℃ of 12h/, 12h) germinates, and notes during this time keeping the skin wet.After germinateing 3-4 days, transplant to filling up in the culturing pot of quartz sand.When transplanting, carry out the inoculation of rhizobium.After this, with full nitrogen nutrition liquid (table 1), water.
Table 1 nutrient solution prescription
During to growth of seedling to 10cm left and right, carry out again a legume inoculation.Then, the nitrogen concentration of nutrient solution is reduced to below 5mM/L, carries out the alternately property pouring of one day nutrient solution one sky and water.So continue, in the time of 50 days, just can obtain pink and have the rhizobium of nitrogenase activity.Repeat above experiment three times, detect the output of rhizobium etc., result is as shown in table 2.
Table 2 rhizobium cultivation results
| Method | Dross number/strain | Output g/m 2 | Average growth rate cm/ days |
| Conventional method | 0～200 | 4511 | 1.07 |
| Embodiment method | 300～500 | 5089 | 1.47 |
When understanding, above-mentioned embodiment is only illustrative explanation, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (1)
1. the cultural method of an alfalfa Phylloxera, it comprises the steps: after alfalfa seed sterilization, be placed in the culture dish that is covered with filter paper, in illumination box, germinate, after germinateing 3-4 days, transplant to filling up in the culturing pot of quartz sand, when transplanting, carry out the inoculation of rhizobium, water with nutrient solution, growth of seedling carries out a legume inoculation during to 10cm left and right again, then, reduce the nitrogen concentration of nutrient solution to 5mM, carry out the alternately property pouring of one day nutrient solution one sky and water, described nutrient solution is formed by major constituents and microcomponent configuration, wherein the content of major constituents and microcomponent is
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| CN201210024784.2A CN102523787B (en) | 2012-02-06 | 2012-02-06 | Culture method for increasing number and activity of alfalfa root nodules |
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| CN201210024784.2A CN102523787B (en) | 2012-02-06 | 2012-02-06 | Culture method for increasing number and activity of alfalfa root nodules |
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| CN102523787A CN102523787A (en) | 2012-07-04 |
| CN102523787B true CN102523787B (en) | 2014-04-16 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105144911B (en) * | 2015-09-28 | 2017-10-17 | 江苏农林职业技术学院 | A kind of straw or like vegetable cultural method |
| CN105660108A (en) * | 2016-01-08 | 2016-06-15 | 山西省农业科学院高寒区作物研究所 | High-yield cultivation method for alfalfa in semi-arid region |
| CN105950520B (en) * | 2016-07-18 | 2019-05-10 | 武汉市农业科学技术研究院作物科学研究所 | A kind of rhizobium of efficient phosphate-solubilizing and its application |
| CN115474521B (en) * | 2022-09-22 | 2023-07-25 | 中国农业科学院草原研究所 | Alfalfa root system cultivation method based on nitrogen form |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0242474A2 (en) * | 1986-04-21 | 1987-10-28 | W.R. Grace & Co.-Conn. | Rhizobium inoculants |
| CN101194567A (en) * | 2008-01-03 | 2008-06-11 | 中国农业大学 | Method for improving production volume and quality of alfalfa seed |
-
2012
- 2012-02-06 CN CN201210024784.2A patent/CN102523787B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0242474A2 (en) * | 1986-04-21 | 1987-10-28 | W.R. Grace & Co.-Conn. | Rhizobium inoculants |
| CN101194567A (en) * | 2008-01-03 | 2008-06-11 | 中国农业大学 | Method for improving production volume and quality of alfalfa seed |
Non-Patent Citations (6)
| Title |
|---|
| 刘丽馥.苜蓿根瘤菌在不同基质中与紫花苜蓿共生匹配的研究.《东北农业大学硕士学位论文》.2008, |
| 刘彦江 等.紫花苜蓿应用根瘤菌剂和稀土肥料效果试验.《草业科学》.2005,第22卷(第7期), |
| 李旭军 等.紫花苜蓿专用根瘤菌剂使用技术研究.《草业科学》.2005,第22卷(第5期), |
| 紫花苜蓿专用根瘤菌剂使用技术研究;李旭军 等;《草业科学》;20050531;第22卷(第5期);第28-31页 * |
| 紫花苜蓿应用根瘤菌剂和稀土肥料效果试验;刘彦江 等;《草业科学》;20050731;第22卷(第7期);第27-28页 * |
| 苜蓿根瘤菌在不同基质中与紫花苜蓿共生匹配的研究;刘丽馥;《东北农业大学硕士学位论文》;20080229;第14页、第15页和第60页附录A，第51页第1段 * |
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