CN105983004A - Composition capable of alleviating asthenopia and preparation method - Google Patents
Composition capable of alleviating asthenopia and preparation method Download PDFInfo
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Abstract
The invention discloses a composition capable of alleviating asthenopia and a preparation method, belonging to the field of health food. The composition capable of alleviating asthenopia is prepared from cranberries, xanthophylls, chrysanthemum flowers and the like. The composition and the preparation method have the advantages that the formula is reasonable, the process is scientific, the extract is good in stability and can be used for preparing various preparations for oral administration, the dose is small, and the effect in alleviating asthenopia is obvious.
Description
Technical field
The present invention relates to a kind of composition for relieving asthenopia and preparation method, belong to field of health care food.
Background technology
Asthenopia is due to bad different regarding working environment, visual organ dysfunction, whole body organic disease and psychology
Often etc. factor is single or one group of syndrome caused by interacting, persistently watches attentively after distance objective, makes eyestrain go out
The oculopathy of the symptoms such as swollen, ophthalmalgia, dizziness, eye socket distending pain of losing face.
Asthenopic symptom has that eyestrain, eye are dry and astringent, foreign body sensation, eyelid sense of heaviness, blurred vision, photophobia are shed tears,
Ophthalmic bloated pain and eye are congested etc., severe patient it may also occur that headache, giddy, nauseating, lethargy, absent minded,
Hypomnesis, inappetence and the whole body syndrome such as neck shoulder aching pain in waist and back and articulations digitorum manus numbness, small number of patients can
Diplopia, stereoscopic vision dysfunction, intraocular pressure rising, corneal injury etc. occur, teenager can also occur myopia or
Deepen original myopic degree.Have glaucoma, eye surface or anterior ocular segment disease patient also can cause because of the fatigue of eye or
Increase the weight of original oculopathy.Expert claims, and under normal circumstances, intra tear can form a tunic to people, covers at cornea
With the surface of conjunctiva, referred to as tear film.After people blinks, one layer of tear film can be formed every time, eyes can be kept to moisten
With comfortable, therefore it is not likely to produce the symptoms such as eye is dry, tired.If but staring at a target for a long time (as with computer, seen
Book), the persistent period is oversize, and winking reflex is fewer, it is impossible to form tear film in time, may result in eye surface and is dried, draws
Play asthenopia.
By treatment function, ophthalmic remedy is divided into that resisting fatigue is dry and astringent, cataract, infection three major types and other class (include resisting
Scorching antiallergic, glaucoma, mydriasis medication), wherein resisting fatigue dry and astringent account for 47.69% the market share.Resisting fatigue is done
Puckery ophthalmic product is mainly based on chemical drugs, and accounting 79.45%, next account for the Chinese patent medicine in 1/4th markets exactly.
It is little that health product occupy share, only accounts for 0.02%, and other classes are also only 0.16%.In the dry and astringent product of resisting fatigue, change
Learn medicine class and be sure to occupy first, be mostly based on compositions such as Aspartic Acid, the quick dimension of naphthalene, hyaluronate sodium, chondroitin sulfate.
But containing preservative in most of eye drops, ocular may be injured, need to use with caution.And depend on medicament for the eyes unduly
The blinding oculopathy such as water or select unaccommodated collyrium may increase the weight of xerophthalmia, even induction keratitis.Additionally,
If long-time, altofrequency ground uses and more can damage cell slimy in ocular, destroy the normal physiological merit of tear
Can, ultimately result in ocular surface injury and increase the weight of xerophthalmia.
Summary of the invention
First technical problem that the invention solves the problems that is to provide a kind of composition for relieving asthenopia.
Another technical problem that the invention solves the problems that is to provide the preparation method of above-mentioned composition for relieving asthenopia.
For achieving the above object, the present invention is by the following technical solutions:
A kind of composition for relieving asthenopia, is made up of the raw material of following parts by weight: Pericarpium Citri tangerinae 200-400 part, Wan Shou
Chrysanthemum 20-60 part, Flos Chrysanthemi Indici 10-20 part, Fructus Lycii 10-30 part.
Described composition for relieving asthenopia is made up of the raw material of following parts by weight: Pericarpium Citri tangerinae 300 parts, Flos Tagetis Erectae 40
Part, Flos Chrysanthemi Indici 15 parts, Fructus Lycii 20 parts.
Pericarpium Citri tangerinae is the mature fruit of ericad Pericarpium Citri tangerinae Vaccinium vitis-idaea L., containing anthocyanin, pectin,
The Multiple components such as tannin, arbutin, vitamin C and vitamin B group.In Pericarpium Citri tangerinae rich in anthocyanidin be potent anti-
Oxidant, can suppress to destroy the enzyme of ocular cell, remove the free radical of infringement ocular angiogenesis, maintains normal cell even
Connect, stablize blood vessel, prevent various ophthalmic, to glaucoma, cataract, retinal hemorrhage, diabetic retinopathy,
The ocular disease such as pigmentosa retina and nyctalopia, macula lutea hardening, retinal hemorrhage have specially good effect.Pericarpium Citri tangerinae can improve eye blood
Liquid circulates, and strengthens microvascular maintenance, recovers normal intraocular tension, the supply of the sufficient nutrient substance of offer.To near-sighted, false
The property ocular disease effect such as myopia, amblyopia is obvious.Pericarpium Citri tangerinae can accelerate the ability that rhodopsin is lived again, rhodopsin night just
Necessary to vision.Also can improve dark adaptation disease, improve the recovery capability of eyestrain.
Phylloxanthin is a kind of natural materials being widely present in the plants such as vegetable, flowers, fruit, has another name called " plant Huang
Voxel ", it is amphiblestroid primary pigments composition, medical experiment proves that natural carotenol is the antioxidation of a kind of excellent performance
Agent, Main Function includes: absorbs harmful light such as blue light and protects the eyes from phototoxis, delay the aging of eyes and
Prevent pathological changes；Protection, enhancement vision, promote the regeneration of rhodopsin in retina cell, prevent laser high myopia and regard
Ablatio retinae；Alleviate asthenopia symptom；Improve macular pigment optical density, protect macula lutea, promote that macula lutea is grown；Prevention Huang
Speckle degeneration and retinitis pigmentosa；Reduce the generation etc. of glass-film wart.Adding a certain amount of phylloxanthin in food can
Prevention cell ageing and biological organs are old and feeble, the most also can prevent the vision that senile eyeball retina macular degeneration causes
Decline with blind.
Fructus Lycii has significant function of improving eyesight, and be particularly good at improving eyesight, so being also commonly called as " bright eye ".Ancient Chinese medicine doctor is controlled
Treat blurring of vision and nyctalopia that deficiency of liver-blood, deficiency of kidney-YIN cause.
Flos Chrysanthemi is the dry capitulum of feverfew chrysanthemum.It is usually used in anemopyretic cold, dizziness of having a headache, conjunctival congestion and swelling pain, eye
Blurred vision flower, sore and toxic.
The present invention uses modern extraction technique, and above-mentioned medical material is carried out reasonable compatibility and extraction, the active component that obtains and
Its preparation is respectively provided with the asthenopic effect of good alleviation.
Described composition for relieving asthenopia is adopted and is prepared with the following method:
(1) each raw material is weighed by proportioning raw materials, standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 2-3 time respectively, each 8-10 times amount extraction 1-2 hour, extracting solution is respectively
Merging and concentrate, at being concentrated into 50-60 DEG C, relative density is 1.05-1.30, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds acid extraction 2-3 time of pH=0.5-5, and each 8-15 times amount is extracted 0.5-1 hour, merges
Extracting solution, crossing aperture is the NF membrane of 0.5-3nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.05-1.30,
It is spray-dried, obtains fine powder C；
(4) Flos Tagetis Erectae adds aqueous solution 30-50 DEG C containing 0.1-3% yeast and extracts 24-120 hour, adds volume basis
Than for 40-90% ethanol extraction 3 times, each 6-8 times amount is extracted 1-2 hour, and extracting solution concentrates, and is concentrated into 50-60
DEG C time relative density be 1.05-1.30, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
Described composition for relieving asthenopia preferably employs following method and prepares:
(1) each raw material is weighed by proportioning raw materials, standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 2-3 time respectively, each 8-10 times amount extraction 1-2 hour, extracting solution is respectively
Merging and concentrate, at being concentrated into 50-60 DEG C, relative density is 1.10-1.20, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds acid extraction 2-3 time of pH=1-3, and each 8-15 times amount is extracted 0.5-1 hour, and merging carries
Taking liquid, crossing aperture is the NF membrane of 1-2nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.10-1.20, spraying
It is dried, obtains fine powder C；
(4) Flos Tagetis Erectae adds aqueous solution 35-45 DEG C containing 0.5-2% yeast and extracts 48-96 hour, adds percent by volume
For 50-80% ethanol extraction 3 times, each 6-8 times amount is extracted 1-2 hour, and extracting solution concentrates, and is concentrated into 50-60 DEG C
Time relative density be 1.10-1.20, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
Described composition for relieving asthenopia is most preferably adopted and is prepared with the following method:
(1) each raw material is weighed by proportioning raw materials, standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 3 times respectively, each 10 times amount extraction 1 hour, extracting solution merges respectively
Concentrating, at being concentrated into 50-60 DEG C, relative density is 1.15, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds the acid extraction 2 times of pH=1.5, and each 15 times amount are extracted 1 hour, united extraction liquid, mistake
Aperture is the NF membrane of 1nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.10, is spray-dried, obtains fine powder
C；
(4) Flos Tagetis Erectae adds aqueous solution 35 DEG C containing 1% yeast and extracts 72 hours, and adding percent by volume is 70% second
Alcohol extraction 3 times, each 6 times amount extraction 2 hours, extracting solution concentrates, and when being concentrated into 50-60 DEG C, relative density is 1.10,
Lyophilization, obtains dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
Described composition for relieving asthenopia is possibly together with adjuvant.
Described adjuvant is selected from starch, microcrystalline Cellulose, sucrose, aspartame, dextrin, lactose, glucose, chlorination
Sodium, sodium carboxymethyl cellulose, crospolyvinylpyrrolidone, magnesium stearate, micropowder silica gel, vitamin C, half Guang
In propylhomoserin, citric acid, mannitol, vertical compression lactose, malic acid, xylitol, maltodextrin, essence and sodium sulfite
Any one or a few.
Described composition for relieving asthenopia is granule, powder, tablet, capsule or oral liquid.
The preparation method of a kind of composition for relieving asthenopia, comprises the following steps:
(1) Pericarpium Citri tangerinae 200-400 part, Flos Tagetis Erectae 20-60 part, Flos Chrysanthemi Indici 10-20 part, Fructus Lycii 10-30 part are weighed standby
With；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 2-3 time respectively, each 8-10 times amount extraction 1-2 hour, extracting solution is respectively
Merging and concentrate, at being concentrated into 50-60 DEG C, relative density is 1.05-1.30, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds acid extraction 2-3 time of pH=0.5-5, and each 8-15 times amount is extracted 0.5-1 hour, merges
Extracting solution, crossing aperture is the NF membrane of 0.5-3nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.05-1.30,
It is spray-dried, obtains fine powder C；
(4) Flos Tagetis Erectae adds aqueous solution 30-50 DEG C containing 0.1-3% yeast and extracts 24-120 hour, adds volume basis
Than for 40-90% ethanol extraction 3 times, each 6-8 times amount is extracted 1-2 hour, and extracting solution concentrates, and is concentrated into 50-60
DEG C time relative density be 1.05-1.30, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
The preparation method of preferred a kind of composition for relieving asthenopia, comprises the following steps:
(1) Pericarpium Citri tangerinae 300 parts, Flos Tagetis Erectae 40 parts, Flos Chrysanthemi Indici 15 parts, Fructus Lycii 20 parts are weighed standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 2-3 time respectively, each 8-10 times amount extraction 1-2 hour, extracting solution is respectively
Merging and concentrate, at being concentrated into 50-60 DEG C, relative density is 1.10-1.20, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds acid extraction 2-3 time of pH=1-3, and each 8-15 times amount is extracted 0.5-1 hour, and merging carries
Taking liquid, crossing aperture is the NF membrane of 1-2nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.10-1.20, spraying
It is dried, obtains fine powder C；
(4) Flos Tagetis Erectae adds aqueous solution 35-45 DEG C containing 0.5-2% yeast and extracts 48-96 hour, adds percent by volume
For 50-80% ethanol extraction 3 times, each 6-8 times amount is extracted 1-2 hour, and extracting solution concentrates, and is concentrated into 50-60 DEG C
Time relative density be 1.10-1.20, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
It is excellent that the present invention uses optimization technique to extract acquisition active constituents of medicine, the such as Pericarpium Citri tangerinae stability in acid flux material
In neutral or basic solvent, acid extraction is used to improve stability and extraction ratio；It is purified by NF membrane technique,
Go the removal of impurity, improve the content of effective ingredient；The traditional extraction process of phylloxanthin is organic reagent heating extraction, reagent
, there is potential safety hazard in inflammable, explosive and high volatility, the present invention uses yeast to extract operation safety, work in production
Skill is simple, extraction ratio is high, low cost.
Cyclic nucleotide is that a class is widely present in the intracellular important active substances of all living things, cAMP therein,
CGMP by the effect of its " second message,second messenger ", regulates the information transmission of many hormones and neurotransmitter, ginseng especially
The cellular metabolism link important with many.CAMP Yu eGMP is a pair antagonistic substance, participates in the regulation and control of cell effect,
The two is contrary to the regulation effect of cellular metabolism reaction and physiological function, and concentration change is correlated with mutually on the contrary.Excessive eye,
Producing asthenopia symptom, make ciliary muscle generation spastic contractions, in ciliary muscle, cAMP reduces, and cGMP increases.Real
Testing research to show: in asthenopia model black eye rabbit corpus ciliare, cAMP content declines, cGMP content raises.
Use the present composition, cAMP in asthenopia Rabbits Models ciliary muscle can be significantly inhibited and reduce and cGMP
The effect increased, presents significant dose-effect relationship trend.Additionally, the present composition is to xerophthalmia rabbit lacrimal secretion
There is facilitation, thus alleviate asthenopic symptom.
The invention have the advantage that reasonable recipe of the present invention, preparation method is simple, craft science, and extract good stability can
For preparing various oral formulations, take safety, have no side effect, alleviate asthenopia effect notable.
Describe technical solution of the present invention by the following examples in detail, do not limit the practical range of the present invention with this.
Detailed description of the invention
Embodiment 1: preparation composition for relieving asthenopia
One. prescription:
Pericarpium Citri tangerinae 300g, Flos Tagetis Erectae 40g, Flos Chrysanthemi Indici 15g, Fructus Lycii 20g
Two. preparation method:
(1) Pericarpium Citri tangerinae 300g, Flos Tagetis Erectae 40g, Flos Chrysanthemi Indici 15g, Fructus Lycii 20g are weighed, standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 3 times respectively, each 10 times amount extraction 1 hour, extracting solution merges respectively
Concentrating, at being concentrated into 50-60 DEG C, relative density is 1.15, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds the acid extraction 2 times of pH=1.5, and each 15 times amount are extracted 1 hour, united extraction liquid, mistake
Aperture is the NF membrane of 1nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.10, is spray-dried, obtains fine powder
C；
(4) Flos Tagetis Erectae adds aqueous solution 35 DEG C containing 1% yeast and extracts 72 hours, and adding percent by volume is 70% second
Alcohol extraction 3 times, each 6 times amount extraction 2 hours, extracting solution concentrates, and when being concentrated into 50-60 DEG C, relative density is 1.10,
Lyophilization, obtains dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
Finally give dried object I 25g, dried object II 5g, active component 30g.
Embodiment 2: preparation composition for relieving asthenopia
One. prescription: Pericarpium Citri tangerinae 3000g, Flos Tagetis Erectae 400g, Flos Chrysanthemi Indici 150g, Fructus Lycii 200g
Two. preparation method:
(1) Pericarpium Citri tangerinae 3000g, Flos Tagetis Erectae 400g, Flos Chrysanthemi Indici 150g, Fructus Lycii 200g are weighed standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 2 times respectively, 10 times amount that add water for the first time are extracted 2 hours, add for the second time
Water 8 times amount is extracted 1.5 hours, and extracting solution merges concentration respectively, and at being concentrated into 50-60 DEG C, relative density is 1.18, subtracts
Pressure dry, pulverize, and obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds the acid extraction 3 times of pH=1, and each 10 times amount are extracted 0.5 hour, united extraction liquid, mistake
Aperture is the NF membrane of 2nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.10, is spray-dried, obtains fine powder
C；
(4) Flos Tagetis Erectae adds aqueous solution 40 DEG C containing 1.5% yeast and extracts 96 hours, and adding percent by volume is 60%
Ethanol extraction 3 times, each 8 times amount extraction 1.5 hours, extracting solution concentrates, and when being concentrated into 50-60 DEG C, relative density is
1.15, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
Finally give dried object I 260g, dried object II 60g, active component 320g.
Embodiment 3: preparation composition for relieving asthenopia
One. prescription: Pericarpium Citri tangerinae 200g, Flos Tagetis Erectae 60g, Flos Chrysanthemi Indici 10g, Fructus Lycii 10g
Two. preparation method:
(1) Pericarpium Citri tangerinae 200g, Flos Tagetis Erectae 60g, Flos Chrysanthemi Indici 10g, Fructus Lycii 10g are weighed standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 2 times respectively, each 8 times amount extraction 1 hour, extracting solution merges dense respectively
Contracting, at being concentrated into 50-60 DEG C, relative density is 1.05, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds the acid extraction 2 times of pH=0.5, and each 8 times amount are extracted 0.5 hour, united extraction liquid,
Crossing the NF membrane that aperture is 0.5nm, when filtrate is concentrated into 50-60 DEG C, relative density is 1.05, is spray-dried, obtains
Fine powder C；
(4) Flos Tagetis Erectae adds aqueous solution 30-50 DEG C containing 0.1% yeast and extracts 24 hours, and adding percent by volume is 40%
Ethanol extraction 3 times, each 6 times amount extraction 1 hour, extracting solution concentrates, and when being concentrated into 50-60 DEG C, relative density is
1.05, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
Embodiment 4: preparation composition for relieving asthenopia
One. prescription: Pericarpium Citri tangerinae 400g, Flos Tagetis Erectae 20g, Flos Chrysanthemi Indici 20g, Fructus Lycii 30g
Two. preparation method:
(1) Pericarpium Citri tangerinae 400g, Flos Tagetis Erectae 20g, Flos Chrysanthemi Indici 20g, Fructus Lycii 30g are weighed standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 3 times respectively, each 10 times amount extraction 2 hours, extracting solution merges respectively
Concentrating, at being concentrated into 50-60 DEG C, relative density is 1.30, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds the acid extraction 3 times of pH=5, and each 15 times amount are extracted 1 hour, united extraction liquid, mistake
Aperture is the NF membrane of 3nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.30, is spray-dried, obtains fine powder
C；
(4) Flos Tagetis Erectae adds aqueous solution 30-50 DEG C containing 3% yeast and extracts 120 hours, and adding percent by volume is 90%
Ethanol extraction 3 times, each 8 times amount extraction 2 hours, extracting solution concentrates, and when being concentrated into 50-60 DEG C, relative density is
1.30, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
Embodiment 5: preparation composition for relieving asthenopia
One. prescription: Pericarpium Citri tangerinae 300g, Flos Tagetis Erectae 30g, Flos Chrysanthemi Indici 10g, Fructus Lycii 20g
Two. preparation method:
(1) Pericarpium Citri tangerinae 300g, Flos Tagetis Erectae 30g, Flos Chrysanthemi Indici 10g, Fructus Lycii 20g are weighed standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 2 times respectively, each 8 times amount extraction 1 hour, extracting solution merges dense respectively
Contracting, at being concentrated into 50-60 DEG C, relative density is 1.20, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds the acid extraction 3 times of pH=3, and each 10 times amount are extracted 0.5 hour, united extraction liquid, mistake
Aperture is the NF membrane of 2nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.20, is spray-dried, obtains fine powder
C；
(4) Flos Tagetis Erectae adds aqueous solution 35 DEG C containing 0.5% yeast and extracts 48 hours, and adding percent by volume is 50%
Ethanol extraction 3 times, each 6 times amount extraction 2 hours, extracting solution concentrates, and when being concentrated into 50-60 DEG C, relative density is
1.20, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
Embodiment 6: preparation composition for relieving asthenopia
One. prescription: Pericarpium Citri tangerinae 250g, Flos Tagetis Erectae 50g, Flos Chrysanthemi Indici 20g, Fructus Lycii 30g
Two. preparation method:
(1) Pericarpium Citri tangerinae 250g, Flos Tagetis Erectae 50g, Flos Chrysanthemi Indici 20g, Fructus Lycii 30g are weighed standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 3 times respectively, each 10 times amount extraction 2 hours, extracting solution merges respectively
Concentrating, at being concentrated into 50-60 DEG C, relative density is 1.15, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds the acid extraction 3 times of pH=2, and each 12 times amount are extracted 1 hour, united extraction liquid, mistake
Aperture is the NF membrane of 2nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.15, is spray-dried, obtains fine powder
C；
(4) Flos Tagetis Erectae adds aqueous solution 45 DEG C containing 2% yeast and extracts 96 hours, and adding percent by volume is 80% second
Alcohol extraction 3 times, each 7 times amount extraction 1.5 hours, extracting solution concentrates, and when being concentrated into 50-60 DEG C, relative density is
1.15, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
Embodiment 7: preparation composition for relieving asthenopia
One. prescription: Pericarpium Citri tangerinae 300g, Flos Tagetis Erectae 25g, Flos Chrysanthemi Indici 15g, Fructus Lycii 10g
Two. preparation method:
(1) Pericarpium Citri tangerinae 300g, Flos Tagetis Erectae 25g, Flos Chrysanthemi Indici 15g, Fructus Lycii 10g are weighed standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 3 times respectively, each 10 times amount extraction 1.5 hours, extracting solution closes respectively
And concentrate, at being concentrated into 50-60 DEG C, relative density is 1.17, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds the acid extraction 2 times of pH=2, and each 12 times amount are extracted 0.5 hour, united extraction liquid, mistake
Aperture is the NF membrane of 1nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.15, is spray-dried, obtains fine powder
C；
(4) Flos Tagetis Erectae adds aqueous solution 35-45 DEG C containing 2.5% yeast and extracts 60 hours, and adding percent by volume is 70%
Ethanol extraction 3 times, each 6 times amount extraction 1 hour, extracting solution concentrates, and when being concentrated into 50-60 DEG C, relative density is
1.13, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
Embodiment 8: prepare capsule
The dried object I 25g that embodiment 1 obtains, adds dextrin 10g, wet granulation, adds dried object II 5g mixing
Uniformly, encapsulating capsule, obtain capsule, every dress 0.4g.
Embodiment 9: prepare tablet
Embodiment 2 obtains active component 320g, adds lactose 180g, maltodextrin 100g, stevioside 0.5%, indigo plant
Certain kind of berries essence 0.01%, dry granulation, tabletting, obtain buccal tablet, every tablet weight 1.2g.
Embodiment 10: zoopery
One. test material
1. laboratory animal:
Black eye rabbit, male, cleaning grade, Zhejiang University of Traditional Chinese Medicine's Experimental Animal Center provides.
New zealand rabbit, male, cleaning grade, Zhejiang University of Traditional Chinese Medicine's Experimental Animal Center provides.
2. medicine and reagent:
Alleviate asthenopia extract, prepare by embodiment 1, Zhongxin Innova Laboratories.
Lycium-rehmannia sheet, Xiuzheng Pharmaceutical Group Co., Ltd.
Rabbit cyclic adenosine monophosphate (cAMP) ELISA detection kit, Shanghai history is auspicious can bio tech ltd.
Rabbit cyclic guanosine monophosphate (cGMP) ELISA detection kit, Shanghai history is auspicious can bio tech ltd.
Atropine injection, 0.5mg/ml, Tianjin KingYork Amino Acid Co., Ltd..
Schirmer tests, Eagle Vision.
3. instrument:
High speed refrigerated centrifuge, Contifuge 17RS, Heraeus.
Microplate reader, IEC Micro CL 17 Thermo ELECTRON CORPORATION.
Two. method and result
1. the impact on asthenopia Rabbits Models corpus ciliare tissue cAMP Yu cGMP
Select healthy without hindering black eye rabbit, the most male, in addition to Normal group, light cycle the most round the clock, daytime 30lux,
Night, 0lux, continuous 6d made asthenopia model.By animal pattern equilibrium packet: model control group, Lycium-rehmannia sheet group,
Alleviating asthenopia extract group (2,4,8g crude drug/kg), by body weight gastric infusion, it is pure that matched group gives equal-volume
Water, successive administration three weeks.After last is administered 24 hours, sacrificed by decapitation, rapidly extraction bilateral eyeball, micro-in operation
Mirror bearing segmentation from corpus ciliare, homogenate, centrifugal, take supernatant, euzymelinked immunosorbent assay (ELISA) (ELISA) measure cAMP and
CGMP content.Use SPSS software to carry out one factor analysis of variance and data are carried out statistical procedures.Result of the test
Refer to table 1.
Table 1 alleviates the impact on asthenopia Rabbits Models corpus ciliare tissue cAMP Yu cGMP of the asthenopia extract
Compared with Normal group, ##P < 0.01；Compared with model control group, * P < 0.05；**P<0.01
Clinic shows, excessively with eye, makes ciliary muscle generation spastic contractions, and in ciliary muscle, cAMP reduces, and cGMP
Increase.Result of the test shows: compared with Normal group, in model control group black eye rabbit corpus ciliare under cAMP content
Fall, cGMP raises, prompting modeling success；Comparing with model control group, 2g crude drug/kg and the alleviation of above dosage regard
Tired extract can significantly inhibit cAMP in asthenopia Rabbits Models ciliary muscle to be reduced and cGMP increase, presents aobvious
The dose-effect relationship trend write.
2. Tiredness model rabbit lacrimal secretion dry on eye impact
Select healthy new zealand rabbit, the most male, by body weight equilibrium packet: Normal group, Lycium-rehmannia sheet group,
Alleviating asthenopia extract group (2,4,8g crude drug/kg), by body weight gastric infusion, it is pure that matched group gives equal-volume
Water, successive administration three weeks.3rd week first day, the method with reference to Burgalassi lachrymal gland injecting atropine carried out tear
Secrete the modeling that low eye is dry tired, eyes equal glandula lacrimalis inferior injecting atropine, 0.25ml/ eye, every day 4 times, continuous 7
My god.Monitoring index is the measurement of lacrimal secretion, and the Schirmer test determination time is 2 minutes.Use SPSS software
Carry out one factor analysis of variance and data are carried out statistical procedures.Result of the test refers to table 2.
Table 2 alleviates the impact on dry Tiredness model rabbit eyes Schirmer of the asthenopia extract
Compared with model control group, * P < 0.05；**P<0.01
Result shows, 4g crude drug/kg and above dosage alleviate asthenopia extract can significantly inhibit induced by atropine
The minimizing of rabbit eyes Schirmer, and present significant dose-effect relationship trend.It is dry to eye that asthenopia extract is alleviated in prompting
Tiredness model rabbit lacrimal secretion has certain facilitation, thus alleviates asthenopic symptom.
Claims (10)
1. a composition for relieving asthenopia, it is characterised in that be made up of the raw material of following parts by weight: Pericarpium Citri tangerinae
200-400 part, Flos Tagetis Erectae 20-60 part, Flos Chrysanthemi Indici 10-20 part, Fructus Lycii 10-30 part.
A kind of composition for relieving asthenopia the most according to claim 1, it is characterised in that by following parts by weight
Raw material make: Pericarpium Citri tangerinae 300 parts, Flos Tagetis Erectae 40 parts, Flos Chrysanthemi Indici 15 parts, Fructus Lycii 20 parts.
A kind of composition for relieving asthenopia the most according to claim 1 and 2, it is characterised in that described alleviation regards
Tired compositions is adopted and is prepared with the following method:
(1) each raw material is weighed by proportioning raw materials, standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 2-3 time respectively, each 8-10 times amount extraction 1-2 hour, extracting solution is respectively
Merging and concentrate, at being concentrated into 50-60 DEG C, relative density is 1.05-1.30, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds acid extraction 2-3 time of pH=0.5-5, and each 8-15 times amount is extracted 0.5-1 hour, merges
Extracting solution, crossing aperture is the NF membrane of 0.5-3nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.05-1.30,
It is spray-dried, obtains fine powder C；
(4) Flos Tagetis Erectae adds aqueous solution 30-50 DEG C containing 0.1-3% yeast and extracts 24-120 hour, adds volume basis
Than for 40-90% ethanol extraction 3 times, each 6-8 times amount is extracted 1-2 hour, and extracting solution concentrates, and is concentrated into 50-60
DEG C time relative density be 1.05-1.30, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
A kind of composition for relieving asthenopia the most according to claim 3, it is characterised in that: described alleviation regards tired
The compositions of labor is adopted and is prepared with the following method:
(1) each raw material is weighed by proportioning raw materials, standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 2-3 time respectively, each 8-10 times amount extraction 1-2 hour, extracting solution is respectively
Merging and concentrate, at being concentrated into 50-60 DEG C, relative density is 1.10-1.20, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds acid extraction 2-3 time of pH=1-3, and each 8-15 times amount is extracted 0.5-1 hour, and merging carries
Taking liquid, crossing aperture is the NF membrane of 1-2nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.10-1.20, spraying
It is dried, obtains fine powder C；
(4) Flos Tagetis Erectae adds aqueous solution 35-45 DEG C containing 0.5-2% yeast and extracts 48-96 hour, adds percent by volume
For 50-80% ethanol extraction 3 times, each 6-8 times amount is extracted 1-2 hour, and extracting solution concentrates, and is concentrated into 50-60 DEG C
Time relative density be 1.10-1.20, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
A kind of composition for relieving asthenopia the most according to claim 4, it is characterised in that: described alleviation regards tired
The compositions of labor is adopted and is prepared with the following method:
(1) each raw material is weighed by proportioning raw materials, standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 3 times respectively, each 10 times amount extraction 1 hour, extracting solution merges respectively
Concentrating, at being concentrated into 50-60 DEG C, relative density is 1.15, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds the acid extraction 2 times of pH=1.5, and each 15 times amount are extracted 1 hour, united extraction liquid, mistake
Aperture is the NF membrane of 1nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.10, is spray-dried, obtains fine powder
C；
(4) Flos Tagetis Erectae adds aqueous solution 35 DEG C containing 1% yeast and extracts 72 hours, and adding percent by volume is 70% second
Alcohol extraction 3 times, each 6 times amount extraction 2 hours, extracting solution concentrates, and when being concentrated into 50-60 DEG C, relative density is 1.10,
Lyophilization, obtains dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
A kind of composition for relieving asthenopia the most according to claim 3, it is characterised in that: described alleviation regards tired
The compositions of labor is possibly together with adjuvant.
A kind of composition for relieving asthenopia the most according to claim 3, it is characterised in that: described adjuvant is selected from
Starch, microcrystalline Cellulose, sucrose, aspartame, dextrin, lactose, glucose, sodium chloride, carboxymethyl cellulose
Sodium, crospolyvinylpyrrolidone, magnesium stearate, micropowder silica gel, vitamin C, cysteine, citric acid, sweet
Any one or a few in dew alcohol, vertical compression lactose, malic acid, xylitol, maltodextrin, essence and sodium sulfite.
A kind of composition for relieving asthenopia the most according to claim 3, it is characterised in that: described alleviation regards
Tired compositions is granule, powder, tablet, capsule or oral liquid.
9. the preparation method of a composition for relieving asthenopia, it is characterised in that comprise the following steps:
(1) Pericarpium Citri tangerinae 200-400 part, Flos Tagetis Erectae 20-60 part, Flos Chrysanthemi Indici 10-20 part, Fructus Lycii 10-30 part are weighed standby
With；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 2-3 time respectively, each 8-10 times amount extraction 1-2 hour, extracting solution is respectively
Merging and concentrate, at being concentrated into 50-60 DEG C, relative density is 1.05-1.30, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds acid extraction 2-3 time of pH=0.5-5, and each 8-15 times amount is extracted 0.5-1 hour, merges
Extracting solution, crossing aperture is the NF membrane of 0.5-3nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.05-1.30,
It is spray-dried, obtains fine powder C；
(4) Flos Tagetis Erectae adds aqueous solution 30-50 DEG C containing 0.1-3% yeast and extracts 24-120 hour, adds volume basis
Than for 40-90% ethanol extraction 3 times, each 6-8 times amount is extracted 1-2 hour, and extracting solution concentrates, and is concentrated into 50-60
DEG C time relative density be 1.05-1.30, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
The preparation method of a kind of composition for relieving asthenopia the most according to claim 8, it is characterised in that bag
Include following steps:
(1) Pericarpium Citri tangerinae 300 parts, Flos Tagetis Erectae 40 parts, Flos Chrysanthemi Indici 15 parts, Fructus Lycii 20 parts are weighed standby；
(2) Flos Chrysanthemi, Fructus Lycii extracting in water 2-3 time respectively, each 8-10 times amount extraction 1-2 hour, extracting solution is respectively
Merging and concentrate, at being concentrated into 50-60 DEG C, relative density is 1.10-1.20, drying under reduced pressure, pulverizes, obtains fine powder A, B；
(3) Pericarpium Citri tangerinae adds acid extraction 2-3 time of pH=1-3, and each 8-15 times amount is extracted 0.5-1 hour, and merging carries
Taking liquid, crossing aperture is the NF membrane of 1-2nm, and when filtrate is concentrated into 50-60 DEG C, relative density is 1.10-1.20, spraying
It is dried, obtains fine powder C；
(4) Flos Tagetis Erectae adds aqueous solution 35-45 DEG C containing 0.5-2% yeast and extracts 48-96 hour, adds percent by volume
For 50-80% ethanol extraction 3 times, each 6-8 times amount is extracted 1-2 hour, and extracting solution concentrates, and is concentrated into 50-60 DEG C
Time relative density be 1.10-1.20, lyophilization, obtain dried object I；
(5) by fine powder A, B, C mix homogeneously, dried object II is obtained；
(6) dried object I, II are mixed, be active component.
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