CN105942493A - Chinese wolfberry fruit and poria cocos capsules and preparation method thereof - Google Patents
Chinese wolfberry fruit and poria cocos capsules and preparation method thereof Download PDFInfo
- Publication number
- CN105942493A CN105942493A CN201610272873.7A CN201610272873A CN105942493A CN 105942493 A CN105942493 A CN 105942493A CN 201610272873 A CN201610272873 A CN 201610272873A CN 105942493 A CN105942493 A CN 105942493A
- Authority
- CN
- China
- Prior art keywords
- poria
- medlar
- capsule
- extract
- capsules
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002775 capsule Substances 0.000 title claims abstract description 74
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 235000013399 edible fruits Nutrition 0.000 title abstract description 5
- 244000241872 Lycium chinense Species 0.000 title abstract 3
- 235000015468 Lycium chinense Nutrition 0.000 title abstract 3
- 235000008599 Poria cocos Nutrition 0.000 title abstract 3
- 244000197580 Poria cocos Species 0.000 title abstract 3
- 239000000284 extract Substances 0.000 claims abstract description 60
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims abstract description 36
- 210000000582 semen Anatomy 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 20
- 229960003080 taurine Drugs 0.000 claims abstract description 18
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 claims abstract description 13
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 claims abstract description 13
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 claims abstract description 12
- 235000013734 beta-carotene Nutrition 0.000 claims abstract description 12
- 239000011648 beta-carotene Substances 0.000 claims abstract description 12
- 229960002747 betacarotene Drugs 0.000 claims abstract description 12
- 239000000314 lubricant Substances 0.000 claims abstract description 11
- 239000008187 granular material Substances 0.000 claims abstract description 6
- 241001619461 Poria <basidiomycete fungus> Species 0.000 claims description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 15
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 14
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical group [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 12
- 239000008518 lycium barbarum polysaccharide Substances 0.000 claims description 10
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 9
- 239000000377 silicon dioxide Substances 0.000 claims description 7
- 235000012239 silicon dioxide Nutrition 0.000 claims description 7
- 229930003944 flavone Natural products 0.000 claims description 6
- 235000011949 flavones Nutrition 0.000 claims description 6
- 235000019359 magnesium stearate Nutrition 0.000 claims description 6
- 238000005453 pelletization Methods 0.000 claims description 6
- 150000002213 flavones Chemical class 0.000 claims description 5
- 238000012805 post-processing Methods 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 36
- 230000036039 immunity Effects 0.000 abstract description 22
- 230000003647 oxidation Effects 0.000 abstract description 11
- 238000007254 oxidation reaction Methods 0.000 abstract description 11
- 239000002994 raw material Substances 0.000 abstract description 9
- 238000001035 drying Methods 0.000 abstract description 4
- 238000012856 packing Methods 0.000 abstract description 4
- 238000011049 filling Methods 0.000 abstract description 3
- 238000005498 polishing Methods 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
- 238000004154 testing of material Methods 0.000 abstract 1
- 238000005303 weighing Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 description 41
- 241000699666 Mus <mouse, genus> Species 0.000 description 29
- 241000699670 Mus sp. Species 0.000 description 29
- 230000006870 function Effects 0.000 description 25
- 238000012360 testing method Methods 0.000 description 23
- 230000037396 body weight Effects 0.000 description 19
- 210000002966 serum Anatomy 0.000 description 18
- 230000003078 antioxidant effect Effects 0.000 description 16
- 239000013642 negative control Substances 0.000 description 14
- 230000036541 health Effects 0.000 description 13
- 150000003254 radicals Chemical class 0.000 description 13
- 241000700159 Rattus Species 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 12
- 230000002708 enhancing effect Effects 0.000 description 12
- 241001465754 Metazoa Species 0.000 description 11
- 238000010171 animal model Methods 0.000 description 11
- 239000000047 product Substances 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 10
- 230000003064 anti-oxidating effect Effects 0.000 description 9
- 210000000952 spleen Anatomy 0.000 description 9
- 238000012109 statistical procedure Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 206010020751 Hypersensitivity Diseases 0.000 description 7
- 208000026935 allergic disease Diseases 0.000 description 7
- 230000007815 allergy Effects 0.000 description 7
- 230000003111 delayed effect Effects 0.000 description 7
- 230000001965 increasing effect Effects 0.000 description 7
- 230000000242 pagocytic effect Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 238000003304 gavage Methods 0.000 description 6
- 230000036737 immune function Effects 0.000 description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 5
- 230000032683 aging Effects 0.000 description 5
- 239000003963 antioxidant agent Substances 0.000 description 5
- 230000006378 damage Effects 0.000 description 5
- -1 decolours Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 239000007902 hard capsule Substances 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 5
- 210000001541 thymus gland Anatomy 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 108010062580 Concanavalin A Proteins 0.000 description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 210000002683 foot Anatomy 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000011068 loading method Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 239000002671 adjuvant Substances 0.000 description 3
- 230000003712 anti-aging effect Effects 0.000 description 3
- 235000006708 antioxidants Nutrition 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 235000013402 health food Nutrition 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 238000010348 incorporation Methods 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000000976 ink Substances 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 210000002540 macrophage Anatomy 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 210000001616 monocyte Anatomy 0.000 description 3
- 210000000822 natural killer cell Anatomy 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000010992 reflux Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000002000 scavenging effect Effects 0.000 description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 description 3
- 210000001835 viscera Anatomy 0.000 description 3
- 239000000080 wetting agent Substances 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 235000002710 Ilex cornuta Nutrition 0.000 description 2
- 241001310146 Ilex cornuta Species 0.000 description 2
- 235000010326 Osmanthus heterophyllus Nutrition 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 229960003067 cystine Drugs 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000004438 eyesight Effects 0.000 description 2
- 238000000556 factor analysis Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- 208000033065 inborn errors of immunity Diseases 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000003340 mental effect Effects 0.000 description 2
- 210000002864 mononuclear phagocyte Anatomy 0.000 description 2
- 230000036542 oxidative stress Effects 0.000 description 2
- 238000005502 peroxidation Methods 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 208000028529 primary immunodeficiency disease Diseases 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 239000003826 tablet Substances 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- LIFNDDBLJFPEAN-BPSSIEEOSA-N (2s)-4-amino-2-[[(2s)-2-[[2-[[2-[[(2s)-5-amino-2-[[(2s)-2-[[(2s)-6-amino-2-[[(2s)-2-[[(2s)-5-oxopyrrolidine-2-carbonyl]amino]propanoyl]amino]hexanoyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]acetyl]amino]acetyl]amino]-3-hydroxypropanoyl]amino Chemical compound NC(=O)C[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@@H]1CCC(=O)N1 LIFNDDBLJFPEAN-BPSSIEEOSA-N 0.000 description 1
- YHVQIDWAIRCSOQ-UHFFFAOYSA-N 1-nitrotetrazol-2-ium chloride Chemical compound [Cl-].[O-][N+](=O)N1C=[NH+]N=N1 YHVQIDWAIRCSOQ-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 101710186708 Agglutinin Proteins 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 102100024222 B-lymphocyte antigen CD19 Human genes 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- 230000005778 DNA damage Effects 0.000 description 1
- 231100000277 DNA damage Toxicity 0.000 description 1
- 241000255601 Drosophila melanogaster Species 0.000 description 1
- 241000628997 Flos Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 101000980825 Homo sapiens B-lymphocyte antigen CD19 Proteins 0.000 description 1
- 101710146024 Horcolin Proteins 0.000 description 1
- 206010020852 Hypertonia Diseases 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 241000581650 Ivesia Species 0.000 description 1
- 101710189395 Lectin Proteins 0.000 description 1
- 244000241838 Lycium barbarum Species 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 101710179758 Mannose-specific lectin Proteins 0.000 description 1
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 1
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 1
- 101000981253 Mus musculus GPI-linked NAD(P)(+)-arginine ADP-ribosyltransferase 1 Proteins 0.000 description 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241000283898 Ovis Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 241000700157 Rattus norvegicus Species 0.000 description 1
- 241000576755 Sclerotia Species 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 101710185318 Thymic factor Proteins 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000910 agglutinin Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 229960004397 cyclophosphamide Drugs 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000003195 fascia Anatomy 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- YAGKRVSRTSUGEY-UHFFFAOYSA-N ferricyanide Chemical compound [Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YAGKRVSRTSUGEY-UHFFFAOYSA-N 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 239000000745 gonadal hormone Substances 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000001497 healthy food Nutrition 0.000 description 1
- 235000009200 high fat diet Nutrition 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229960001438 immunostimulant agent Drugs 0.000 description 1
- 239000003022 immunostimulating agent Substances 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 239000011812 mixed powder Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 229950006238 nadide Drugs 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 150000002926 oxygen Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 229960002275 pentobarbital sodium Drugs 0.000 description 1
- 210000003024 peritoneal macrophage Anatomy 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- NNFCIKHAZHQZJG-UHFFFAOYSA-N potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000007348 radical reaction Methods 0.000 description 1
- 230000007420 reactivation Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 235000021067 refined food Nutrition 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000005464 sample preparation method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035943 smell Effects 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 235000011088 sodium lactate Nutrition 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009747 swallowing Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 210000003371 toe Anatomy 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 230000005760 tumorsuppression Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to Chinese wolfberry fruit and poria cocos capsules and a preparation method thereof. The capsules comprise hollow capsules and contents enclosed in the hollow capsules. The contents are prepared from the following components in parts by weight: 80-120 parts of Chinese wolfberry fruit extract, 80-120 parts of poria cocos extract, 80-120 parts of semen cuscutae extract, 70-100 parts of taurine, 3-7 parts of beta-carotene, 6-10 parts of a glidant, and 2-6 parts of a lubricant. The preparation of the capsules consists of the processes as follows: raw material testing, sieving, weighing, granulating, drying, granule separating, filling, polishing, inner packing, outer packing, storing, etc. Compared with the prior art, the capsules can enhance immunity and prevent oxidation at the same time, and are quick in release, good in absorption effect, convenient in use, simple in processes and low in costs.
Description
Technical field
The present invention relates to a kind of health-related capsule, especially relate to a kind of Chinese holly with enhancing immunity and anti-oxidation function
Qi Indian buead capsule.
Background technology
Immunity refers to a kind of specific physiological reaction of body contact antigenicity foreign body or dissident's composition, and it is that body is entering
A kind of important physiological function identifying self, pushing aside or excluding persons of different views obtained during change.Immunologic function is to maintain body health, defence
External effective barrier with the various harmful species in inherence.Keep the normal immunological state of body, to preventing various diseases be
Vital.Body's immunity can constantly be affected by various factors, such as physics harmful in living environment, chemicals
Matter effect, the invasion and attack etc. of bacterial virus etc..Additionally, the Nervous and Mental Factors of self, such as spirit hypertonicity, fatigue, work pressure
The increasing of power, people generally feel tired, do not have enough sleep, and work energy is the most vigorous, and body's immunity all can be caused to lack of proper care,
Reduce the resistivity to disease, panimmunity Indexes Abnormality in body.Show as internal natural killer cell (NK) function to press down
System and quantity reduce, monocyte function obstacle, and immunoglobulin synthesis is suppressed.Research shows, the Asia of about 31% is good for
Kang Renqun exists IgG, IgM, IgD, IgA to be reduced.
Owing to social competition is more and more fierce, increasing from the pressure of every aspect, it is in sub-health population continuous
Increasing, " subhealth state " is referred to as the threat of 21 century human maximum.According to World Health Organization (WHO) one global investigation display, full generation
The people of boundary's true health only has 5%, and the most ill people the most only about 20%, and the people of 75% is in sub-health state.
Domestic have survey data to show, meets health standards person and accounts for about the 15% of total crowd, is diagnosed as disease, no in crowd
Healthy person also accounts for about 15%, and sub-health population accounts for about the 60% of total crowd, the most mainly middle-aged population, accounts for
48-59%, high level intellectual, company manager's subhealth state incidence rate may be up to 70%.
Human body generation pathological changes and aging complicated mechanism, scientific circles propose all old and feeble theoretical, " tissue oxidation " i.e.
It is one of them.It is thought that the body of human body is in metabolic processes, peroxide, hydrogen peroxide, hydroxyl can be generated too much
The materials such as group, and then cause a large amount of generations of free radical.Free radical then can damage each structure histiocytic, such as cell
Film, Cytoplasm and endonuclear many materials.Many studies have shown that, radical reaction is the pathology base of numerous disease and damage
Plinth, is also the major reason accelerating body aging.Under normal circumstances, there is many in body and plant the thing of defence radical damage
Matter, i.e. antioxidant reductase and antioxidant so that interior free yl is constantly eliminated, to protect body cell not damaged by free radical
Wound.When senility of humanbody, remove the reduced capability of free radical.Superfluous free radical initiation toxic action a series of biology, tissue
The function of organ progressively gets muddled and obstacle etc..
Since it is old and feeble relevant with oxidation, then to manage to change the oxidation states of human body, just can prevent and reduce disease undoubtedly,
Slow down aging.
Scientific research finds, setting up in human body has a kind of special Antioxidative Defense System, here it is be full of the anti-of human body
The only various antioxidative enzymes of free radical harm, they occur DNA impaired and carry out in time in where in constantly inspection human body
Repair.Due to the existence of these ferment, human body always maintains basic antioxidative functional.But, life also exists again many
The oxidation of cell can be accelerated, strengthen the factor of Free Radical, such as ultraviolet and radiation.Hobby tobacco and wine, high fat diet,
Processed food is edible too much, excessive movement, mental burden etc., and these factors can be helped make it from bad to worse, and makes free radical produce in a large number and more
Add active.Therefore, the Antioxidation Mechanism of human body itself is only leaned on can't effectively to prevent and stop the oxidation of body.On the one hand,
People should pay attention to avoiding the oxidation of body.On the one hand, should actively seek foreign aid, this foreign aid's strength is exactly that people are daily to be contacted
Food, there is the food of antioxidation.
Along with improving constantly of living standards of the people, health is increasingly paid attention to by people, sees a doctor from the ill of past
Be changed into anosis prevention, increasing people by supplement in good time some targetedly health product improve self immune merit
Can, to reach the health secured good health, based on this, society needs a kind of health care food with enhancing immunity and anti-oxidation function badly
Product.
Summary of the invention
Defect that the purpose of the present invention is contemplated to overcome above-mentioned prior art to exist and provide one to have and strengthen simultaneously
Immunity and anti-oxidation function, and discharge fast, good absorbing effect, easy to use and preparation technology simple, the Chinese holly of low cost
Qi Indian buead capsule and preparation method thereof.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of medlar-poria capsule, this capsule includes Capsules and encloses the content in Capsules, and described is interior
The tolerant component by following weight portion content is made:
Preferably, described content is made up of the component of following weight portion content:
Described Fructus Lycii extract contains the lycium barbarum polysaccharide of 30～40wt%.
This Fructus Lycii extract uses following methods to prepare: by Fructus Lycii crushed after being dried, add volumetric concentration be 75～
The ethanol solution of 95%, reflux, extract, 2～5 times, united extraction liquid, decolours, concentrates, prepares Fructus Lycii extract after drying.
Described Poria extract contains the pachyman of 20～30wt%.
This Poria extract uses following methods to prepare: by Poria crushed after being dried, and adding volumetric concentration is 75～95%
Ethanol solution, reflux, extract, 2～5 times, united extraction liquid, decolour, concentrate, prepare Poria extract after drying.
Described Semen Cuscutae extract contains the total flavones of 10～20wt%.
This Semen Cuscutae extract uses following methods to prepare: by Semen Cuscutae crushed after being dried, add volumetric concentration be 75～
The ethanol solution of 95%, reflux, extract, 2～5 times, united extraction liquid, decolours, concentrates, prepares Semen Cuscutae extract after drying.
Described fluidizer is silicon dioxide, and described lubricant is magnesium stearate.
Adding above-mentioned adjuvant (fluidizer and lubricant) can make capsule 's content have good mobility, it is ensured that medicated powder is fast
Insert capsule fastly, and ensure the stability of content uniformity.
Affect normal operating and the mobility that principal element is implant of loading amount accuracy that hard capsule is filled, and comment
The simplest method of valency mobility represented with angle of repose.Angle of repose is the least, illustrates that frictional force is the least, and mobility is the best, the easiest
In filling capsule.Capsule can be met during general α≤40 ° angle of repose and fill the demand of mobility in production process.Hard determining
When fatty acid magnesium and the concrete consumption of silicon dioxide, we have done the following test being numbered 1～4, and have measured angle of repose, result respectively
It is shown in Table 1:
Table 1 fluidizer, lubricant quantity select
From above-mentioned experimental result, adding the silicon dioxide of 8g and the magnesium stearate of 4g in every 1000 formula, it is stopped
Only angle α is less than 40 °, and the good fluidity of material can meet medicated powder and insert capsule rapidly, and ensure stablizing of content uniformity
Property.
For verifying above experimental result, filling continuously with capsule filler 30 minutes, randomization 3 times, detection loading amount is poor
Different, every time sampling 10, detects content uniformity, the results are shown in Table 2:
Table 2 capsule loading amount investigates test
From the above results, loading amount is stable.
A kind of preparation method of medlar-poria capsule, the method comprises the following steps:
(1) by Fructus Lycii extract, Poria extract, Semen Cuscutae extract, taurine and beta-carotene according to proportioning mistake
Sieve, weigh and mix；
(2) add mass fraction be 90～95% ethanol water pelletize；
(3) through being dried, adding lubricant and fluidizer by proportioning after granulate after pelletizing, the most always mix, fill also
Medlar-poria capsule is obtained through post processing.
In the described ethanol water in step (2), the mass fraction of ethanol is 95%.
It, as granulation wetting agent, is investigated by ethanol water by being numbered the experiment of 1～3, result such as table 3:
The screening of table 3 wetting agent
| Numbering | Mixed powder amount | Wetting agent | Phenomenon |
| 1 | 390g | 75% ethanol | Viscous sieve, is difficult to pelletize |
| 2 | 390g | 85% ethanol | Viscous sieve, is difficult to pelletize |
| 3 | 390g | 95% ethanol | Easily pelletizing, granularity is preferable |
According to result above, the sample of numbering 3 is pelletized easily, and not viscous sieve, granularity is preferable, second in corresponding ethanol water
The mass fraction of alcohol is 95%.
Always doing time in described step (3) is 20 minutes.
Described post processing includes the techniques such as the polishing on Capsules surface, inner packing, outer package.
When determining total incorporation time, we have done following test, incorporation time are set 10 minutes, 20 minutes, 30 points
Clock, separately sampled 3 times, measures significant composition crude polysaccharides content, the results are shown in Table 4:
Table 4 is always done time selection
In terms of above-mentioned RSD index result, mixing 20 minutes and mix 30 minutes, RSD value is close, in order to save the energy, really
Determining incorporation time is 20 minutes, can reach to mix the purpose all having.
First have to ensure this product no side effects as health food, the health care declared in conjunction with this product, really
This product fixed by Fructus Lycii extract, Poria extract, Semen Cuscutae extract, taurine, beta-carotene be Effect raw material, with
Magnesium stearate, silicon dioxide are adjuvant, and Fructus Lycii, Poria, Semen Cuscutae are that " Ministry of Public Health is about further specification healthy food material
The notice of management " (defend method prison send out [2002] No. 51) announce " can be used for the article list of health food ", " be i.e. that food is again
The article list of medicine " in medicine；Taurine, beta-carotene are the raw material that can be used for health food.Modern pharmacological research is demonstrate,proved
Bright, above-mentioned raw materials has enhancing immunity, antioxidative function, and the quality standard of above-mentioned raw materials and meets company standard Appendix B
Requirement.Adjuvant magnesium stearate, silicon dioxide are as fluidizer, and for allowing the raw material used in GB2760, its quality standard accords with
Close enterprise's mark appendix C.
Fructus Lycii is the dry mature fruit of plant of Solanaceae lycium barbarum Lycium barbarum L., and nature and flavor are sweet flat, return liver
Kidney channel.There is the function of nourishing the liver and kidney, replenishing vital essence to improve eyesight.Fructus Lycii described in Compendium of Material Medica " hard muscles and bones, remove asthenia, mend vital essence ".
Lycium barbarum polysaccharide is primary bioactive components therein, has immunomodulating, antitumor, anti-ageing various biological effect of waiting for a long time.
Experiment shows, lycium barbarum polysaccharide can increase total T cell and TH subgroup percentage ratio, improves lymhocyte transformation rate；To cyclophosphamide
The immunologic hypofunction reaction induced, lycium barbarum polysaccharide also has return action, makes total T cell of reduction, TH, TH/Ts and pouring turn
Rate is returned to normally or close to normal control values.Lycium barbarum polysaccharide does not has the work of proliferative induction to the most activated lymphocyte
With；Lymphopoiesis for activating through PHA and PMA respectively has significant facilitation, and this shows Fructus Lycii T, B to activation
The propagation of lymphocyte has facilitation.And lycium barbarum polysaccharide is remarkably improved internal activities of antioxidant enzymes, scavenging activated oxygen
And anti-lipid peroxidation.In sum, Fructus Lycii extract has enhancing immunity and antioxidative function.
Poria is the dry sclerotia that porous section transverse hole fungus belongs to fungus Poria, and its medical value is the most on the books in Ancient Times in China.
Poria main chemical compositions is polysaccharide and triterpene, and effect is quite varied.It is many that Poria has tumor suppression, immunostimulant, antiinflammatory etc.
The pharmacological action of aspect.Experiment shows, Poria extract can significantly improve the cytophagous phagocytic activity of hypoimmunity mice, energy
Stimulate spleen, the increase of thymic factor D injection, the delayed allergy of hypoimmunity mice can be remarkably promoted, mice can be significantly improved
Antibody-producting cell number, and mice serum agglutinin level can be obviously enhanced.Oral pachyman can significantly resist
The change of CD3+, CD19+ cell proportion in PPs, MLNs, especially obvious to PPs effect, intestinal is glued by prompting pachyman
The immune effect of film is better than effect immune to periphery.Pachyman extract has stronger oxidation resistance,
In the system removing DPPH and in reducing power system, the Scavenging activity of sample, all more than VC, shows that it has stronger resisting
Oxidability.Pachyman can improve the oxidation resistance of fruit bat, anti-lipid peroxidation, extends life span of drosophila melanogaster.To sum up institute
Stating, Poria extract has enhancing immunity and antioxidative function
Semen Cuscutae extract is traditional traditional tonic medicine, the compound such as the resinous glycoside of chemical composition master, saccharide, flavonoid,
There is antagonism body free radical produce and strengthen the effects such as immunologic function.Experiment shows, Semen Cuscutae can be remarkably reinforced aging model
The hematid immunity function of mice, has delaying senility function.Semen Cuscutae can promote that mouse immune organ spleen, thymus increase,
And improve macrophage phagocytic function；Promote lymphproliferation response；Induction interleukin produces.Semen Cuscutae extract can make little
The Y-Shaped electric maze of Mus is tested correct number of times and is significantly reduced, and can improve Mice brain tissues SOD, GSH-PX vigor, reduces containing of MDA
Amount, its anti-aging effects may reduce lipid peroxide MDA contain by improving cerebral tissue antioxidase SOD, GSH-PX vigor
Measure and realize.The antioxidant activity of the flavone in Semen Cuscutae extract is better than Vc and BHT, is that a kind of natural effective free radical is clear
Except agent.Semen Cuscutae extract can improve the activity of aging rat neurocyte antioxidant enzyme, reduces Radical Metabolism product
Content, there is certain anti-aging effects.In sum, Semen Cuscutae extract has enhancing immunity and antioxidative merit
Energy.
Taurine is a kind of nonprotein amino acid, is not involved in the biosynthesis of vivo protein matter, but with cystine, half
The metabolism of cystine is closely related.Taurine is the important substance of regulation body normal physiological function, can improve the spy of body
The opposite sex and non-specific immunity.Experiment shows, taurine can strengthen the phagocytic function of mononuclear phagocyte, improves body fluid and exempts from
Epidemic disease ability, has certain promotion to immune function of mice；The spleen index of immunosuppressed mice, thymus index can be dramatically increased,
Promote spleen lymphocyte proliferation, improve antibody-producting cell number and half hemolysis value, strengthen the phagocytic function of mononuclear phagocyte.
Taurine can strengthen the oxidation resistance of DM renal cortex of rats, alleviates DM renal cortex of rats oxidative stress (OS)；Reduce DM rat
Serum uric acid level, has part protective effect to its renal function.Ultra-oxygen anion free radical and hydroxy radical are had by taurine
Stronger scavenging action, and increase with the increase of taurine addition within the specific limits.DNA damage is repaiied by taurine
Reactivation power increases with the age and reduces, but is all remarkably higher than matched group at the repair ability of each age group.In sum, taurine
There is enhancing immunity and antioxidative function.
Beta-carotene is that a class can be by plant and Microbe synthesis, but the material that can not synthesize in animal body. it is dynamic
Having multiple effect in object, beta-carotene, as vitamin a source, has the maintenance normal vision of animal, protective epithelium tissue
(skin and mucosa) complete, promotes the formation of gonadal hormone, and protection body tissue or cell are from free radical and lipid peroxidation
The effects such as damage.Test shows, adds optimal dose beta-carotin microcapsule and can significantly improve piglet in sow heavy in pig daily ration
Nascent quality, strengthen the immunologic function of in-pig and total antioxidant capacity.The LPO level of experimental mice declines and SOD
Rise with GSH-Px activity.In sum, carotene has enhancing immunity and antioxidative function.
This product forms selects hard capsule, and hard capsule preparation can cover raw material discomfort flavour and abnormal smells from the patient, and outward appearance is neat and artistic,
Easily swallowing, hard capsule and tablet, pill are different, can be not added with pressure, so disintegrate release is fast in intestines and stomach, inhale during preparation
Receive effective, taking convenience, health, it is simple to carry, storage period long, and production technology is simple, low cost.Therefore this product choosing
Select hard capsule preparation.
Compared with prior art, the present invention selects Fructus Lycii extract, Poria extract, Semen Cuscutae extract, taurine
And beta-carotene is as effective ingredient, this mutual compatibility of several materials, it is possible to work in coordination with and play enhancing immunity and antioxidative work
With.Rapid-action, effect is notable, side effect is little, effect stability, and preparation technology is simple.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
A kind of medlar-poria capsule, this capsule includes Capsules and encloses the content in Capsules, content by
Following components is made: Fructus Lycii extract, Poria extract, Semen Cuscutae extract, taurine, beta-carotene, fluidizer and profit
Lubrication prescription, wherein, fluidizer is silicon dioxide, and lubricant is magnesium stearate.According to raw material, prepare three batches of products, three batches of products
Raw material composition and lot number are as shown in table 5.
Table 5
The preparation method of this medlar-poria capsule, comprises the following steps:
(1) by Fructus Lycii extract, Poria extract, Semen Cuscutae extract, taurine and beta-carotene according to proportioning mistake
Sieve, weigh and mix；
(2) add the ethanol water that mass fraction is 95% to pelletize；
(3) through being dried after pelletizing, add lubricant and fluidizer by proportioning after granulate, total mixed 20 minutes, fill and warp
Cross the techniques such as the polishing on Capsules surface, inner packing, outer package and obtain medlar-poria capsule.
The medlar-poria capsule preparing the present embodiment detects, and result is as follows.
One, enhancing immunity Function detection
1. materials and methods
1.1 samples: the medlar-poria capsule that the present embodiment prepares.
1.2 laboratory animal
The clear great floss ICR male mice that Changchun hundred million this laboratory animal technology Co., Ltd provides, production licence
Number: SCXK-(lucky) 2011-0004.Feedstuff is provided by Changchun hundred million this laboratory animal technology Co., Ltd, production licence
Number: SCXK-(lucky) 2010-0001.This laboratory animal environmental facility quality certification, lucky moving sets word 10-1005, and laboratory animal is with being permitted
Can the number of card: SYXK-(lucky) 2010-0011.Select healthy male mice 48, body weight 18g～22g, be divided into 4 groups, often group 12, make
For immune one group, carry out internal organs/weight ratio pH-value determination pH, half hemolysis value (HC50) mensuration and antibody-producting cell detection；Choosing is strong
Health male mice 48, body weight 18g～22g, it is divided into 4 groups, often group 12, as immune two groups, carries out carbonic clearance experiment；Choosing is strong
Health male mice 48, body weight 18g-22g, it is divided into 4 groups, often group 12, as immune three groups, the mice carrying out ConA induction is drenched
B cell transformation experiment and NK cytoactive detection；Select healthy male mice 48, body weight 18g～22g, be divided into 4 groups, often organize 12
Only, delayed allergy experiment is carried out as immune four g groups；Select healthy male mice 48, body weight 18g～22g, be divided into 4
Group, often group 12, as immune five groups, carry out Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell experiment.
1.3 dosage choice and preparation
Recommending taking dose is 2/day, 2 tablets/time, 0.402 gram/, i.e. 1.6080g/60kg BW, Xiang Ziyu
0.0268/kg BW, arranges given low with 1,10 and 30 times of day recommended intake, i.e. 0.0268,0.2680,0.8040g/
Kg BW, each dosage group dilutes with pure water.Making negative control group with pure water, each group mouse stomach amount is 0.2mL/10g BW, even
Continuous gavage surveyed every immune indexes after 30 days.
1.4 instruments and reagent:
Electronic balance (0.1g), analytical balance, clean bench, titanium dioxide dish incubator, centrifuge, 722 spectrophotometrics
Meter, water bath with thermostatic control, microplate reader, microscope etc.；Sterile surgical instrument, slide gauge (precision 0.02mm), microsyringe (25
μ L) cell counter, the flat Tissue Culture Plate in 24 holes and 96 holes, 96 hole U-shaped cells cultivate pull, glass dish, gauze, test tube,
Slide frame, 200 eye mesh screens, timer, hemoglobin pipet, load glass sheet etc.；Sheep red blood cell (SRBC) (SRBC), normal saline, Hank ' s
Liquid (pH7.2～7.4), RPMI1640 culture fluid, calf serum, mycillin, concanavalin A, Con A (ConA), 1% glacial acetic acid,
The HCl solution of 1mol/L, acid isopropyl alcohol (96mL isopropanol adds 4mL hydrochloric acid), MTT, PBS (pH7.2～7.4), benefit
(carbonic acid gently takes 1.0g, high-potassium ferricyanide 0.2g, potassium cyanide for body (guinea pig serum), SA buffer, agarose, all formula reagent
0.05g, adds distilled water to 1000mL), YAC-1 cell, sodium lactate, nitro tetrazolium chloride, PMS, oxidation
Type nadide, the Tris-HCL buffer (pH8.2) of 0.2mol/L, 1%NP40, india ink, Na2CO3, chicken red blood cell, methanol,
Giemsa dye liquor etc..
1.5 experimental technique
1.5.1 internal organs/weight ratio pH-value determination pH
After mouse weights, dislocation is put to death, and takes spleen and thymus, removes most fascia, blots organ surface blood stains with filter paper, weigh,
Calculate spleen/body weight ratio and thymus/body weight ratio.
1.5.2 delayed allergy (DTH) (the foot sole of the foot thickens method)
Taking Sanguis caprae seu ovis, brine, mice 2% (v/v) SRBC peritoneal immunity, every injected in mice 0.2mL/ is only.
Latter 4 days of immunity, measures left back sufficient sole of the foot portion thickness, and measuring point subcutaneous injection 20% (v/v) SRBC (the 20 every Mus of μ l/), after injection
24h measures left back sufficient sole of the foot portion thickness three times, calculates meansigma methods.
1.5.3 mice carbonic clearance is tested
Mouse tail vein injection, with the india ink of normal saline dilution 4 times, is injected at once, in injecting after prepared Chinese ink the 2nd,
10min, takes blood 20 μ L from angular vein clump respectively, joins 2ml 0.1%Na2CO3In solution, with Na2CO3Compare, use
722 type spectrophotometers colorimetric densitometric value (OD at 600nm wavelength600nm).By sacrifice, take liver, spleen, weigh, meter
Calculation phagocytic index a:
K=(lgOD1-lgOD2)/(t2-t1)
1.6 data statistics
One factor analysis of variance in SPSS 11.5 statistical software is used to carry out average ratio relatively, when variance is neat, between each group two-by-two
Relatively use LSD method, during heterogeneity of variance, compare two-by-two between each group and use Tamhane method.
2 results
The impact on Mouse Weight of 2.1 tested materials
Table 6
P value: each experimental group compares with negative control group
From table 6, through statistical procedures, per os gives tested material 30 days, and each metering group Mouse Weight, weightening finish are with negative
Matched group comparing difference is without significance (P > 0.05).Mouse Weight, weightening finish are had no significant effect by i.e. tested material.
Table 7 tested material is on immune two groups of Mouse Weights impact
P value: each experimental group compares with negative control group
From table 7, through statistical procedures, per os gives tested material 30 days, and each metering group Mouse Weight, weightening finish are with negative
Matched group comparing difference is without significance (P > 0.05).Mouse Weight, weightening finish are had no significant effect by i.e. tested material.
Table 8 tested material is on immune three groups of Mouse Weights impact
P value: each experimental group compares with negative control group
From table 8, through statistical procedures, per os gives tested material 30 days, and each metering group Mouse Weight, weightening finish are with negative
Matched group comparing difference is without significance (P > 0.05).Mouse Weight, weightening finish are had no significant effect by i.e. tested material.
Table 9 tested material is on immune three groups of Mouse Weights impact
P value: each experimental group compares with negative control group
From table 9, through statistical procedures, per os gives tested material 30 days, and each metering group Mouse Weight, weightening finish are with negative
Matched group comparing difference is without significance (P > 0.05).Mouse Weight, weightening finish are had no significant effect by i.e. tested material.
Table 10 tested material is on immune three groups of Mouse Weights impact
P value: each experimental group compares with negative control group
From table 10, through statistical procedures, per os gives tested material 30 days, and each metering group Mouse Weight, weightening finish are with cloudy
Property matched group comparing difference is without significance (P > 0.05).Mouse Weight, weightening finish are had no significant effect by i.e. tested material.
The impact on mice organs/body weight ratio of 2.2 tested materials
The impact on mice organs/body weight ratio of table 11 tested material
Spleen/body weight ratio P1Value: each experimental group compares with negative control group；
Thymus/body weight ratio P2Value: each experimental group compares with negative control group；
From table 11, through statistical procedures, per os gives tested material 30 days, each metering group mouse spleen body weight and breast
Gland/body weight ratio and negative control group comparing difference are without significance (P > 0.05).I.e. tested material to mice organs/body weight ratio without
Significantly affect.
The impact on mouse cell immunologic function of 2.3 tested materials
2.3.1 the tested material impact on mice delayed allergy (DTH)
The impact on mice delayed allergy (DTH) of table 12 tested material
P value: each experimental group compares with negative control group；* P < 0.05, * * P < 0.01
From table 12, through statistical procedures, per os gives tested material 30 days, low dose group toes and negative control group ratio
Relatively no significant difference (P>0.05), middle and high dosage group and negative control group comparing difference have significance (P<0.05).In i.e.,
The tested material of high dose can strengthen the delayed allergy of mice.
The impact on mouse monokaryon-macrophage phagocytic function of 2.4 tested materials
The impact on mouse monokaryon-macrophage phagocytic function of table 13 tested material
P value: each experimental group compares with negative control group；* P < 0.05, * * P < 0.01
From table 13, through statistical procedures, per os gives tested material 30 days, and low dose group carbonic clearance work can be right with feminine gender
Significance (P<0.05) is had without significance (P>0.05), middle and high dosage group and negative control group comparing difference according to group comparing difference.
The tested material of the most middle and high dosage can improve the carbonic clearance ability of the monocytes/macrophages of mice.
3 brief summaries
Per os gives the tested material 30 days of mice various dose, can strengthen mice delayed allergy, can strengthen thin
Born of the same parents' immunologic function, can improve the carbonic clearance ability of mouse monokaryon-macrophage, i.e. strengthen monocytes/macrophages function, to mice
Body weight growth, internal organs/body weight ratio etc., without impact, have the function strengthening immunity.
Two, anti-oxidation function detection
1. material and method
1.1 samples: the medlar-poria capsule that the present embodiment prepares.
1.2 experimental animals:
1.2.1 source: the SPF level animal that Changchun hundred million this laboratory animal technology Co., Ltd provides.
1.2.2 strain: SPF level Wistar rat, male 40, approval card number: SCXK-(lucky) 2011-0004, Mus is age
12 months.
1.2.3 body weight: 360.0～480.0g
1.2.4 rearing conditions: this experimental animal environmental facility quality certification, the lucky dynamic word 10-1005 that sets, experimental animal use is permitted
Can the number of card: SYXK-(lucky) 2010-0011；Temperature 20～22 DEG C, humidity 55～65%；Feedstuff is by this laboratory animal of Changchun hundred million
Technology Co., Ltd provides, the quality certification number: SCXK-(lucky) 2010-0001.
1.3 dosage choice: test sets by 30 times, 10 times, 5 times of Coming-of-Age Day intake i.e. 1.6080g/60kg BW
0.804,0.268, tri-dosage groups of 0.134g/kg BW, separately set aged controls group.
1.4 sample preparations: design requirement according to test dose, each dose of test thing distilled water is prepared, take 8.04 respectively,
2.68,1.34g is dissolved in distilled water to 100mL, mixes constant volume, uses per os gavage mode to give, and each test group gavage amount is
1.0mL/100g BW, aged controls group gives same volume distilled water, every day gavage once.
1.5 test methods: animal is raised 3 days after buying, and before test, is divided at random by old-age group Mus according to MDA content in serum
Be four groups, i.e. aged controls group, 0.804g/kg BW dosetest group, 0.268g/kg BW dosetest group and 0.134g/kg
BW dosetest group.The continuous gavage of each test group 30 days, in the 31st day with 1% pentobarbital sodium normal saline solution, by 5mL/
After kg BW intraperitoneal injection of anesthesia, postcava is taken a blood sample, and measures MDA content and GDH-Px, SOD activity in serum.
1.6 test data is added up:
One factor analysis of variance in SPSS 11.5 statistical software is used to carry out average ratio relatively, when variance is neat, between each group two-by-two
Relatively use LSD method, during heterogeneity of variance, compare two-by-two between each group and use Tamhane method.
2 results
Before 2.1 on-tests, taking tail blood and survey MDA content, (one old by MDA content, experimental animal to be randomly divided into four groups
Age matched group, three test group).Measurement result is shown in Table 14.
MDA content in front aged male rats serum tested by table 14
P value: each test group compares with aged controls group.
From table 14, before test, in each test group and aged controls group serum MDA content do not have significant difference (P >
0.05)
In 2.2 processs of the test, weigh weekly, to adjust gavage amount, and calculate weightening finish, the results are shown in Table 2.
The impact on aged male rats body weight of table 15 sample
P value: each test group compares with aged controls group.
From table 15, difference that each test group starting weight, eventually weight and weightening finish compare with aged controls group that there are no significant (P >
0.05), illustrate that male elder rats body weight is had no by this sample to significantly affect.
2.3 give sample 30 days continuously, and postcava is taken a blood sample, and measure SOD activity in serum, the results are shown in Table 16.
Table 16 sample is on the impact of SOD activity in aged male rats serum
P value: each test group compares with aged controls group, * compares P < 0.05 with aged controls group.
As shown in Table 16,0.263 it is poor that, 0.134g/kg BW dosage group SOD content compares without significance with aged controls group
Different；0.804g/kg BW dosage group SOD content compares with aged controls group significant difference, has SOD in raising serum and lives
The effect of property.
2.4 give tested material 30 days continuously, and postcava is taken a blood sample, and measure MDA content and GSH-Px activity, result in serum
It is shown in Table 17,18.
Table 17 sample is on the impact of MDA content in aged male rats serum
P value: each test group compares with aged controls group, * compares P < 0.05 with aged controls group.
From table 17,0.268, that 0.134g/kg BW dosage group MDA content compares without significance with aged controls group is poor
Different；0.804g/kg BW dosage group MDA content compares with aged controls group significant difference, has MDA in reduction serum and contains
The effect of amount.
Table 18 sample is on the impact of GSH-Px activity in aged male rats serum
P value: each test group compares with aged controls group, * compares P < 0.05 with aged controls group.
As shown in Table 5,0.268,0.134g/kg BW dosage group GSH-Px activity compares without significance with aged controls group
Difference；0.804g/kg BW dosage group GSH-Px activity compares with aged controls group significant difference, has in increasing serum
The effect of GSH-Px activity.
3 brief summaries
Per os give the medlar-poria capsule 0.268 of rat the present embodiment, 0.134,0.804g/kg BW (respectively this sample
Product are grown up the 30 of recommended intake, 10,5 times) 30 days, have MDA content in reduction Aged Male Rat (12 months ages of Mus) serum,
The effect of antioxidase SOD, GSH-Px activity in increasing serum.Medlar-poria capsule prepared by this embodiment has antioxidation merit
Energy.
Embodiment 2
A kind of medlar-poria capsule, this capsule includes Capsules and encloses the content in Capsules, wherein, content
Thing is made up of the component of following weight portion content:
The preparation method of this medlar-poria capsule, comprises the following steps:
(1) by Fructus Lycii extract, Poria extract, Semen Cuscutae extract, taurine and beta-carotene according to proportioning mistake
Sieve, weigh and mix；
(2) add the ethanol water that mass fraction is 90% to pelletize；
(3) through being dried, adding lubricant and fluidizer by proportioning after granulate after pelletizing, always mix 30min, fill and pass through
Post processing obtains medlar-poria capsule.
This medlar-poria capsule has simultaneously enhancing immunity and an anti-oxidation function, and discharges fast, good absorbing effect, makes
Use convenient advantage.
Embodiment 3
A kind of medlar-poria capsule, this capsule includes Capsules and encloses the content in Capsules, wherein content
Thing is made up of the component of following weight portion content:
Wherein, Fructus Lycii extract contains the lycium barbarum polysaccharide of 30wt%, and Poria extract contains the pachyman of 30wt%,
Semen Cuscutae extract contains the total flavones of 10wt%.
The preparation method of this medlar-poria capsule, comprises the following steps:
(1) by Fructus Lycii extract, Poria extract, Semen Cuscutae extract, taurine and beta-carotene according to proportioning mistake
Sieve, weigh and mix；
(2) add the ethanol water that mass fraction is 93% to pelletize；
(3) through being dried, adding lubricant and fluidizer by proportioning after granulate after pelletizing, always mix 25min, fill and pass through
Post processing obtains medlar-poria capsule.
This medlar-poria capsule has simultaneously enhancing immunity and an anti-oxidation function, and discharges fast, good absorbing effect, makes
Use convenient advantage.
Embodiment 4
A kind of medlar-poria capsule, this capsule includes Capsules and encloses the content in Capsules, wherein content
Thing is made up of the component of following weight portion content:
Wherein, Fructus Lycii extract contains the lycium barbarum polysaccharide of 40wt%, and Poria extract contains the pachyman of 20wt%,
Semen Cuscutae extract contains the total flavones of 20wt%.
The preparation method of the medlar-poria capsule of this embodiment is essentially identical with embodiment, and difference is, this enforcement
The proportioning of each material of the content of example.
Embodiment 5
A kind of medlar-poria capsule, this capsule includes Capsules and encloses the content in Capsules, wherein content
Thing is made up of the component of following weight portion content:
Wherein, Fructus Lycii extract contains the lycium barbarum polysaccharide of 35wt%, and Poria extract contains the pachyman of 25wt%,
Semen Cuscutae extract contains the total flavones of 15wt%.
The preparation method of the medlar-poria capsule of this embodiment is essentially identical with embodiment, and difference is, this enforcement
The proportioning of each material of the content of example.
Claims (9)
1. a medlar-poria capsule, this capsule includes Capsules and encloses the content in Capsules, it is characterised in that
Described content is made up of the component of following weight portion content:
A kind of medlar-poria capsule the most according to claim 1, it is characterised in that described content is by following weight portion
The component of content is made:
A kind of medlar-poria capsule the most according to claim 1, it is characterised in that described Fructus Lycii extract contains 30
～the lycium barbarum polysaccharide of 40wt%.
A kind of medlar-poria capsule the most according to claim 1, it is characterised in that described Poria extract contain 20～
The pachyman of 30wt%.
A kind of medlar-poria capsule the most according to claim 1, it is characterised in that described Semen Cuscutae extract contains 10
～the total flavones of 20wt%.
A kind of medlar-poria capsule the most according to claim 1, it is characterised in that described fluidizer is silicon dioxide,
Described lubricant is magnesium stearate.
The preparation method of a kind of medlar-poria capsule the most as claimed in claim 1, it is characterised in that the method includes following step
Rapid:
(1) Fructus Lycii extract, Poria extract, Semen Cuscutae extract, taurine and beta-carotene are sieved according to proportioning,
Weigh and mix；
(2) add mass fraction be 90～95% ethanol water pelletize；
(3) through being dried, adding lubricant and fluidizer by proportioning after granulate after pelletizing, the most always mix, fill and pass through
Post processing obtains medlar-poria capsule.
The preparation method of a kind of medlar-poria capsule the most according to claim 1, it is characterised in that described step (2)
In ethanol water in the mass fraction of ethanol be 95%.
The preparation method of a kind of medlar-poria capsule the most according to claim 1, it is characterised in that described step (3)
In always to do time be 20 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610272873.7A CN105942493A (en) | 2016-04-28 | 2016-04-28 | Chinese wolfberry fruit and poria cocos capsules and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610272873.7A CN105942493A (en) | 2016-04-28 | 2016-04-28 | Chinese wolfberry fruit and poria cocos capsules and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105942493A true CN105942493A (en) | 2016-09-21 |
Family
ID=56915997
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610272873.7A Pending CN105942493A (en) | 2016-04-28 | 2016-04-28 | Chinese wolfberry fruit and poria cocos capsules and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105942493A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109566826A (en) * | 2018-10-15 | 2019-04-05 | 河南省锐达医药科技有限公司 | A kind of anti-aging improves the composition of immunity beauty |
-
2016
- 2016-04-28 CN CN201610272873.7A patent/CN105942493A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109566826A (en) * | 2018-10-15 | 2019-04-05 | 河南省锐达医药科技有限公司 | A kind of anti-aging improves the composition of immunity beauty |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11166998B2 (en) | Eurycoma longifolia extract and its use in enhancing and/or stimulating immune system | |
| CN103169079A (en) | Health-care food with anti-fatigue and immunity-enhancing effects, and preparation method thereof | |
| CN103800390A (en) | Health-care product with immunity-reinforcing and liver-protecting functions, preparation method and application thereof | |
| CN106729136A (en) | A kind of functional food for adjusting immunity | |
| CN110664868A (en) | Composition with anti-glycosylation effect and application thereof | |
| CN101248880B (en) | Fatigue resistant sport drink and method of preparing the same | |
| CN109123612A (en) | A kind of composition with strengthen immunity, health food and preparation method thereof | |
| CN103585400A (en) | Composition having immunity enhancing and fatigue alleviating effects, and preparation method thereof | |
| CN108991529A (en) | The food compositions and preparation method thereof of member are supported in a kind of invigorating the spleen | |
| CN102524799A (en) | Protein powder for improving organism immunity and preparation method thereof | |
| CN102429150A (en) | Vitamin C chewable tablets for enhancing human immunity | |
| CN101684445A (en) | Deer horn blood wine and processing method thereof | |
| CN105942493A (en) | Chinese wolfberry fruit and poria cocos capsules and preparation method thereof | |
| CN102860496A (en) | Oxidation-resisting health-care food for improving immunity and preparation method thereof | |
| CN104585778A (en) | Albumen powder for improving organism immunity and preparation method of albumen powder | |
| CN107281317A (en) | A kind of oral liquid for improving body immunity and preparation method thereof | |
| CN101143203B (en) | Compound oral liquid with liver-protecting and stomach-nourishing function | |
| CN106880784A (en) | It is a kind of with fatigue-relieving, the Chinese medicine composition of anti-aging and its application | |
| CN102885305A (en) | Health food composition for enhancing immunity and preparation method thereof | |
| CN103039977A (en) | Healthcare food with functions of immunity improvement and hypoxia tolerance and preparation method thereof | |
| CN113209217A (en) | Traditional Chinese medicine composition for resisting physical fatigue and preparation method thereof | |
| CN102406172B (en) | Health-care food for enhancing immunity and preparation method thereof | |
| CN101940317B (en) | Health food for improving immunity of human bodies and preparation method thereof | |
| CN104189038A (en) | Traditional Chinese medicine preparation for regulating female physical health and immunity | |
| CN101015345A (en) | Health-care food with hypoxia-resistant and immunity-enhancing functions and its preparing process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160921 |
|
| WD01 | Invention patent application deemed withdrawn after publication |