CN101695255A - Method for cultivating cordyceps sinensis stroma by using hirsutella sinensis - Google Patents
Method for cultivating cordyceps sinensis stroma by using hirsutella sinensis Download PDFInfo
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- CN101695255A CN101695255A CN200910075839A CN200910075839A CN101695255A CN 101695255 A CN101695255 A CN 101695255A CN 200910075839 A CN200910075839 A CN 200910075839A CN 200910075839 A CN200910075839 A CN 200910075839A CN 101695255 A CN101695255 A CN 101695255A
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- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 5
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- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 abstract description 5
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Abstract
The invention belongs to a method for cultivating cordyceps sinensis stroma, and particularly relates to a method for cultivating cordyceps sinensis stroma by using hirsutella sinensis. The method comprises the following steps: soaking maize pulp, soybean meal, wheat grains, rice grains, sorghum grains, silkworm chrysalis meal or crushed whole egg and beef peptone in water; mixing and filtering the soaked raw materials and auxin to obtain filter liquor; and inoculating the soaked materials to the hirsutella sinensis liquor, then cultivating the mixture in dark place, adding the filter liquor to the mixture, and cultivating the mixture in a greenhouse to obtain the cordyceps sinensis stroma. The method has the following advantages that: the asexual hirsutella sinensis strains are inoculated to a manually prepared culture medium so as to grow the stroma in batch, and the stroma can be cyclically grown. The biological conversion rate of the cultivated cordyceps sinensis stroma is about 40 percent, and the cordyceps sinensis stroma contains over 0.02 percent of adenosine C10H13N504 and over 0.10 percent of cordycepin C10H13N503.
Description
Technical field
The invention belongs to the cultural method of Cordyceps sinensis stroma, be specifically related to a kind of method of being cultivated Dietotherapy double-purpose Cordyceps sinensis stroma by the hair spore with China, be exactly that China is by hair spore (Hirsutlla sinensis), on the medium of artificial preparation, cultivate stroma and the method for the results that can circulate, just cultivated the Cordyceps sinensis method of sexual generation by the hair spore with Cordyceps sinensis asexual generation bacterial classification China.
Background technology
Asexual bacterial classification with Cordyceps sinensis, on the medium of artificial preparation, grow Chinese caterpillar fungus complex or stroma, and the results that can circulate, this is the particularly main target of Cordyceps sinensis brainstrust research of fungi worker, experts and scholars still are in research and experimental stage to this both at home and abroad at present.Be the nineteen eighty-three Qinghai herding Shen Nanying of institute professor the earliest, on the experiment base, cultivate the compound stroma of Cordyceps sinensis, be called as " it is the first that Chinese Chinese caterpillar fungus is cultivated stroma " with the isolated asexual bacterial classification of Chinese caterpillar fungus.This bacterial classification is established as China by a hair spore (Hirsutlla sinensis) in the meeting that in October, 2005 China bacteriology can be held in Beijing, be the asexual generation of Cordyceps sinensis C-sinensis.But so far, Shen professor China of no use is further turned out Cordyceps sinensis stroma (also being the sexual generation of Cordyceps sinensis) by the hair spore, and China is also had only asexual generation to pass to the descendant by the hair spore.Year has gone over surplus in the of 20, has thousands of people to study the artificial culture of Chinese caterpillar fungus complex or stroma, is grown stroma by a hair spore with China on the artificial preparation medium so far, and the results that can circulate, and does not appear in the newspapers.
Summary of the invention
The present invention provides an a kind of method with quilt hair spore cultivation Dietotherapy double-purpose Cordyceps sinensis stroma in order to solve the problem that China's quilt hair spore can not be turned out the Cordyceps sinensis stroma and be circulated and gather in the crops in the prior art.
The present invention adopts following technical scheme to realize:
Method with being cultivated Dietotherapy double-purpose Cordyceps sinensis stroma by the hair spore is characterized in that it more than height above sea level 1800m, and day and night temperature carries out in the environment of no industrial pollution more than or equal to 15 ℃, and step is as follows:
The umber of following each raw material is parts by weight.
1), get 30～50 parts of maize pulps, 20～30 parts of analysis for soybean powder, 10～20 parts of wheat berries, 5～10 parts of grain, 5～10 parts of sorghum grains, dried silkworm chrysalis meal or 15～20 parts of crushed whole egg, 15～20 parts of mixing of beef peptone, use mineral water with weight such as mixed materials to be soaked in and must soak material in the same container;
2), former raw material processing: 10～30 parts in former raw material are soaked in the mineral water that is twice in former raw material weight, then with 0.1～0.2 part of mixing of growth hormone, the refrigeration that filters to get filtrate after 20～25 hours is standby, and described growth hormone is formulated by 0.08～0.12 part of Cobastab, 0.08～0.12 part of vitamin E, 180～200 parts of glucose, 180～200 parts in yolk and 800～1200 parts of mineral waters;
3), with the packing of above-mentioned immersion material, seal, sterilize, be cooled to 30 ℃, connect by hair spore liquid strain, per 100 parts are soaked material and connect by in the hair spore liquid 20 parts, put 16～18 ℃ of following lucifuges and cultivate,
4), lucifuge cultivates after 28～33 days, under the condition of sterile working, adds 10 parts of above-mentioned filtrates, carries out hot-house culture then;
5), hot-house culture, keep 10～25 ℃, giving astigmatism stimulated 20 days, every day, the astigmatism irradiation was more than or equal to 10 hours, stroma can be gathered in the crops the first stubble seat after growing ascocarp, sprays 10 parts in growth hormone after collecting again, cultivates by the management method of first batch of temperature and illumination, collect the second stubble seat after 10 days again, can receive the 3rd batch again with quadrat method;
6), 60 ℃ of oven dry of Shou Huo stroma are preserved.
Soak in the glass jar or polypropylene vial that material is sub-packed in height * diameter=10 * 10 centimetre dress material 50 grams in every jar/bottle.
The illumination of astigmatism is 200LX～300LX during hot-house culture.
Maize pulp, analysis for soybean powder, wheat berry, grain, sorghum grain are that crop seeds is made, and must not have the product that chemical industry public hazards and agriculture chemical pollute, if contaminated soil, the product in the environment such as water, air definitely can not be used; Full egg is the whole pulverizing of mountain village peasant with the egg of natural by product and natural food picked from the fields (insect, green grass) raising chicken product; Beef peptone is meant the meat extract that the beef water is stewed, and is moisture less than 20%; Former raw material are meant the matrix material that soil that natural cs was grown or stroma were grown, former raw material can not high-temperature sterilization, can not chemical degerming, keep multiple activation of microorganism in its ecosystem, former first raw material must arrive the natural cs place of production and take, and the former raw material of follow-up use can adopt the matrix material of having turned out stroma; Growth hormone is meant the powder that commercially available vitamin etc. is mixed with; It is the 7-7.5 natural mineral water that mineral water requires PH.
A used bacterial classification China quilt hair spore Hirsutlla sinensis is the asexual generation of Cordyceps sinensis Cordyceps sinensis, can buy from institute of microbiology of the Chinese Academy of Sciences, rather than other bacterial classifications that belong to together, the stroma spore of turning out through the method for the invention is the sexual generation of Cordyceps sinensis.
The present invention requires imitative ecosystem to cultivate, and culture environment requires more than height above sea level 1800m, and day and night temperature is not less than 15 ℃, and ambient atmosphere, soil, water source etc. all do not have industrial pollution; With the canister cultivation, guarantee that subenvironment is nuisanceless.
The present invention has following beneficial effect: on the medium of artificial preparation, connects asexual bacterial classification, grows stroma in batches by the hair spore, and can cycling deposition.The Cordyceps sinensis stroma biological transformation ratio of being cultivated is about 40%, contain adenosine C10H13N5O4 greater than 0.02% (natural cs content is for being no less than 0.01%), contain cordycepin C10H13N5O3 greater than 0.10% (natural cs content trace), two important activity compositions of Chinese caterpillar fungus all are higher than the content of natural cs, Academy of Traditional Chinese Medicine, Shanxi Province pharmacodynamics test shows, this Chinese caterpillar fungus has antifatigue, improves immunity, relieving cough and reducing sputum, liver-protecting and blood fat-reducings etc. are suitable with the natural cs effect, zoopery this product can not surveyed LD50, shows that this product has no side effect.
Embodiment
Embodiment 1:
More than height above sea level 1800m, day and night temperature carries out in the environment of no industrial pollution more than or equal to 15 ℃, and step is as follows: the umber of following each raw material is parts by weight.
1), get 30 parts of maize pulps, 20 parts of analysis for soybean powder, 10 parts of wheat berries, 10 parts of grain, 5 parts of sorghum grains, 20 parts of dried silkworm chrysalis meals, 15 parts of mixing of beef peptone, use mineral water with weight such as mixed materials to be soaked in and must soak material in the same container;
2), former raw material processing: 10 parts in former raw material are soaked in the mineral water that is twice in former raw material weight, then with 0.1 part of mixing of growth hormone, the refrigeration that filters to get filtrate after 25 hours is standby, and described growth hormone is formulated by 0.08 part of Cobastab, 0.12 part of vitamin E, 200 parts of glucose, 180 parts in yolk and 1000 parts of mineral waters;
3), with the packing of above-mentioned immersion material, seal, sterilize, be cooled to 30 ℃, connect by hair spore liquid strain, per 100 parts are soaked material and connect by in the hair spore liquid 20 parts, put 18 ℃ of following lucifuges and cultivate,
4), lucifuge cultivates after 30 days, under the condition of sterile working, adds 10 parts of above-mentioned filtrates, carries out hot-house culture then;
5), hot-house culture, keep 20 ℃, giving astigmatism stimulated 20 days, illumination is 300LX, and astigmatism irradiation every day was more than or equal to 10 hours, and stroma can be gathered in the crops the first stubble seat after growing ascocarp, spray 10 parts in growth hormone after collecting again, management method by first batch of temperature and illumination is cultivated, and collects the second stubble seat after 10 days again, can receive the 3rd batch again with quadrat method;
6), 60 ℃ of oven dry of Shou Huo stroma are preserved.
Soak in the glass jar or polypropylene vial that material is sub-packed in height * diameter=10 * 10 centimetre dress material 50 grams in every jar/bottle.
The Cordyceps sinensis stroma biological transformation ratio of being cultivated 40%, containing adenosine C10H13N5O4 is 0.021%.Containing cordycepin C10H13N5O3 is 0.10%.
Embodiment 2:
More than height above sea level 1800m, day and night temperature carries out in the environment of no industrial pollution more than or equal to 15 ℃,
Step is as follows: the umber of following each raw material is parts by weight.
1), get 50 parts of maize pulps, 25 parts of analysis for soybean powder, 20 parts of wheat berries, 8 parts of grain, 8 parts of sorghum grains, 15 parts of dried silkworm chrysalis meals, 20 parts of mixing of beef peptone, use mineral water with weight such as mixed materials to be soaked in and must soak material in the same container;
2), former raw material processing: 20 parts in former raw material are soaked in the mineral water that is twice in former raw material weight, then with 0.15 part of mixing of growth hormone, the refrigeration that filters to get filtrate after 20 hours is standby, and described growth hormone is formulated by 0.10 part of Cobastab, 0.08 part of vitamin E, 180 parts of glucose, 190 parts in yolk and 800 parts of mineral waters;
3), with the packing of above-mentioned immersion material, seal, sterilize, be cooled to 30 ℃, connect by hair spore liquid strain, per 100 parts are soaked material and connect by in the hair spore liquid 20 parts, put 16 ℃ of following lucifuges and cultivate,
4), lucifuge cultivates after 33 days, under the condition of sterile working, adds 10 parts of above-mentioned filtrates, carries out hot-house culture then;
5), hot-house culture, keep 25 ℃, giving astigmatism stimulated 20 days, illumination is 200LX, and astigmatism irradiation every day was more than or equal to 10 hours, and stroma can be gathered in the crops the first stubble seat after growing ascocarp, spray 10 parts in growth hormone after collecting again, management method by first batch of temperature and illumination is cultivated, and collects the second stubble seat after 10 days again, can receive the 3rd batch again with quadrat method;
6), 60 ℃ of oven dry of Shou Huo stroma are preserved.
Soak in the glass jar or polypropylene vial that material is sub-packed in height * diameter=10 * 10 centimetre dress material 50 grams in every jar/bottle.
The Cordyceps sinensis stroma biological transformation ratio of being cultivated is 41%, and containing adenosine C10H13N5O4 is 0.025%.Containing cordycepin C10H13N5O3 is 0.15%.
Embodiment 3:
More than height above sea level 1800m, day and night temperature carries out in the environment of no industrial pollution more than or equal to 15 ℃, and step is as follows: the umber of following each raw material is parts by weight.
1), get 40 parts of maize pulps, 30 parts of analysis for soybean powder, 15 parts of wheat berries, 5 parts of grain, 10 parts of sorghum grains, 18 parts of crushed whole egg, 18 parts of mixing of beef peptone, use mineral water with weight such as mixed materials to be soaked in and must soak material in the same container;
2), former raw material processing: 30 parts in former raw material are soaked in the mineral water that is twice in former raw material weight, then with 0.2 part of mixing of growth hormone, the refrigeration that filters to get filtrate after 22 hours is standby, and described growth hormone is formulated by 0.12 part of Cobastab, 0.10 part of vitamin E, 190 parts of glucose, 200 parts in yolk and 1200 parts of mineral waters;
3), with the packing of above-mentioned immersion material, seal, sterilize, be cooled to 30 ℃, connect by hair spore liquid strain, per 100 parts are soaked material and connect by in the hair spore liquid 20 parts, put 17 ℃ of following lucifuges and cultivate,
4), lucifuge cultivates after 28 days, under the condition of sterile working, adds 10 parts of above-mentioned filtrates, carries out hot-house culture then;
5), hot-house culture, keep 10 ℃, giving astigmatism stimulated 20 days, illumination is 250LX, and astigmatism irradiation every day was more than or equal to 10 hours, and stroma can be gathered in the crops the first stubble seat after growing ascocarp, spray 10 parts in growth hormone after collecting again, management method by first batch of temperature and illumination is cultivated, and collects the second stubble seat after 10 days again, can receive the 3rd batch again with quadrat method;
6), 60 ℃ of oven dry of Shou Huo stroma are preserved.
Soak in the glass jar or polypropylene vial that material is sub-packed in height * diameter=10 * 10 centimetre dress material 50 grams in every jar/bottle.
The Cordyceps sinensis stroma biological transformation ratio of being cultivated is 40%, and containing adenosine C10H13N5O4 is 0.024%.Containing cordycepin C10H13N5O3 is 0.12%.
Claims (3)
1. a method of being cultivated the Cordyceps sinensis stroma by the hair spore with China is characterized in that it more than height above sea level 1800m, and day and night temperature carries out in the environment of no industrial pollution more than or equal to 15 ℃, and step is as follows:
The umber of following each raw material is parts by weight,
1), get 30～50 parts of maize pulps, 20～30 parts of analysis for soybean powder, 10～20 parts of wheat berries, 5～10 parts of grain, 5～10 parts of sorghum grains, dried silkworm chrysalis meal or 15～20 parts of crushed whole egg, 15～20 parts of mixing of beef peptone, use mineral water with weight such as mixed materials to be soaked in must to soak material in the same container;
2), former raw material processing: 10～30 parts in former raw material are soaked in the mineral water that is twice in former raw material weight, then with 0.1～0.2 part of mixing of growth hormone, the refrigeration that filters to get filtrate after 20～25 hours is standby, and described growth hormone is formulated by 0.08～0.12 part of Cobastab, 0.08～0.12 part of vitamin E, 180～200 parts of glucose, 180～200 parts in yolk and 800～1200 parts of mineral waters;
3), with the packing of above-mentioned immersion material, seal, sterilize, be cooled to 30 ℃, connect by hair spore liquid strain, per 100 parts are soaked material and connect by 20 parts of hair spore liquid strains, put 16～18 ℃ of following lucifuges and cultivate,
4), lucifuge cultivates after 28～33 days, under the condition of sterile working, adds 10 parts of above-mentioned filtrates, carries out hot-house culture then;
5), hot-house culture, keep 10～25 ℃, giving astigmatism stimulated 20 days, every day, the astigmatism irradiation was more than or equal to 10 hours, stroma can be gathered in the crops the first stubble seat after growing ascocarp, sprays 10 parts in growth hormone after collecting again, by the management method of first batch of temperature and illumination, collect the second stubble seat after 10 days again, can receive the 3rd batch again with quadrat method;
6), 60 ℃ of oven dry of Shou Huo stroma are preserved.
2. a method of being cultivated the Cordyceps sinensis stroma by the hair spore with China according to claim 1 is characterized in that described immersion material is sub-packed in the glass jar or polypropylene vial of height * diameter=10 * 10 centimetre, dress material 50 grams in every jar/bottle.
3. according to claim 1 or 2 described methods of being cultivated the Cordyceps sinensis stroma by the hair spore with China, the illumination of astigmatism is 200LX～300LX when it is characterized in that hot-house culture.
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| CN102742454A (en) * | 2012-06-05 | 2012-10-24 | 安徽农业大学 | Artificial culture method of cordyceps longissima sporocarp |
| CN102870600A (en) * | 2012-10-17 | 2013-01-16 | 山西万海澳生物科技有限责任公司 | Cordyceps militaris fruit body cultivation technology for stabilizing cordycepin content |
| CN103371050A (en) * | 2012-04-19 | 2013-10-30 | 薛刚 | Method for cultivating cordyceps sinensis |
| CN104145719A (en) * | 2014-09-04 | 2014-11-19 | 重庆市中药研究院 | Cordyceps sinensis mycelium fermentation production method |
| CN106613314A (en) * | 2016-10-10 | 2017-05-10 | 贵州高山六芝园种植有限公司 | Artificial cordyceps stroma ripening method |
-
2009
- 2009-10-26 CN CN2009100758390A patent/CN101695255B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103371050A (en) * | 2012-04-19 | 2013-10-30 | 薛刚 | Method for cultivating cordyceps sinensis |
| CN102742454A (en) * | 2012-06-05 | 2012-10-24 | 安徽农业大学 | Artificial culture method of cordyceps longissima sporocarp |
| CN102742454B (en) * | 2012-06-05 | 2013-09-04 | 安徽农业大学 | Artificial culture method of cordyceps longissima sporocarp |
| CN102870600A (en) * | 2012-10-17 | 2013-01-16 | 山西万海澳生物科技有限责任公司 | Cordyceps militaris fruit body cultivation technology for stabilizing cordycepin content |
| CN104145719A (en) * | 2014-09-04 | 2014-11-19 | 重庆市中药研究院 | Cordyceps sinensis mycelium fermentation production method |
| CN106613314A (en) * | 2016-10-10 | 2017-05-10 | 贵州高山六芝园种植有限公司 | Artificial cordyceps stroma ripening method |
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