CN102845221A - Fermentation method of Cordyceps sinensis - Google Patents
Fermentation method of Cordyceps sinensis Download PDFInfo
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Abstract
The invention relates to a fermentation method of Cordyceps sinensis. The method simulates growth environments and conditions of wild Cordyceps sinensis to produce fermented Cordyceps sinensis mycelia and products the same as those of the wild Cordyceps sinensis. The method includes: inoculating Hiresutella sinensis strain to a fermentation tank for fermentation after slant culture, and determining medium for culture and fermentation according to nutrients of larvae of host Hepialus armoricanus. The Hiresutella sinensis strain is subjected to slant culture, shaking culture, seed tank culture and fermenter culture, and the medium used for all culture contains larva powder of yellow mealworm, which is used as a medium nitrogen source added with vitamins B1 and B2. The fermented mycelia produced by the fermentation method is the same as or similar to that of the natural wild Cordyceps sinensis in terms of appearance and effective ingredients.
Description
Technical background
Cordyceps sinensis mainly is grown near 3000 meters snow lines to the alpine meadow shrub zone of 5000m of Qinghai-Tibet height above sea level the forest grassy marshland or careless slope.It is that aweto by Ascomycota Clavicipitaceae Cordyceps parasitizes the bat moth larvae in the alpine meadow soil, absorb the nutriment of bat moth larvae in-vivo tissue, larva is ossify, under optimum conditions, go out long bar-shaped stroma and form (being the fruit body of aweto and the complex that bombys batryticatus sclerotium (larva corpse) consists of) by bombys batryticatus head end pumping summer.
Cordyceps sinensis is that polypide links to each other with stroma and forms, 9～12 centimetres of total lengths, and the colour cast crineous, there are many horizontal wrinkles at the back side, and polypide is light and crisp, frangibility, section white, little flexible; Stroma grows from the head of polypide, is 4～7Cm, and base portion is coated with the head of polypide, is long bar-shaped, upper coarse and lower fine, taupe or pitchy, and matter is pliable and tough, and section mostly is hollow.The tool straw mushroom fragrance of smelling, it is sweet to distinguish the flavor of.Stroma Dan Sheng, elongated cylindrical, long 4 to 7 centimetres, 3 millimeters of diameters; The surface dark-brown has thin vertical wrinkle to sepia, and slightly expand on top, and matter is pliable and tough, the section off-white color.The little raw meat of gas, mildly bitter flavor.
The pharmacological effect position of Cordyceps sinensis is stroma and sclerotium (being host's dry polypide), 1757 the Wu Yi Lip river " new compilation of materia medica " begin to see on the books: " labor of reducing phlegm is had a rest for Gan Pingbao lung, kidney-nourishing hemostasis." traditional Chinese medical science is as Yang-strengthening drug, control phlegm and retained fluid, breathe heavily cough, void is breathed heavily, consumptive disease is coughed, spitting of blood, spontaneous sweating, impotence and seminal emission, soreness of waist and knee joint, void is multiple etc. for a long time after being ill.The Pharmacopoeia of the People's Republic of China (version in 2000, one one) is put down in writing its function and is: " invigorate the lung and the kidney hemostasis and phlegm.Be used for that chronic cough void is breathed heavily, consumptive disease is coughed spitting of blood, impotence and seminal emission, soreness of waist and knee joint.”。
Low owing to narrow, the natural parasitic rate of wild cordyceps Distribution Area, harsh to the living environment conditional request, so resource own is more limited.Again because Cordyceps sinensis main product ground ecotope suffers that the people is heavy damage, a large amount of blindly unreasonable excavating cause resource increasingly to reduce in recent years, and output descends year by year.And Cordyceps sinensis is because can medicine-food two-purpose, and people constantly find the pharmacological action that it is new, thus a plurality of country to its multiplication that needs, price goes up year by year, the international market is day by day in short supply.
CN 10215061A discloses a kind of breeding method of cordyceps sinensis, is yellow mealworm and the earthworm that lives are broken into slurries with beater, joins in the fresh milk, make the nutrition base, add again brewer's yeast, corn starch, potato starch, Semen Ginkgo powder, band fish meal, the loach digested tankage, grape powder, growth hormone, lysine, aspartoyl, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, inferior western sour sodium, Cobastab 1 grade is cultivated worm grass product.But this technology is not the acquisition cordyceps mycelia just for obtaining cordyceps militaris sporocarp, and the medium component complexity, and the cordyceps sporophore of producing is in chemical composition and active substance and natural worm summer difference to some extent.
Hirsutella sinensis (Hirsutella sinensis) bacterial classification is to separate to obtain from the fresh Cordyceps sinensis of Yushu district, Qinghai, through biological reducing check, is uniquely so far can finish the Cordyceps sinensis bacteria strain of whole history of life under artificial environment.
CN 101530042A discloses a kind of hirsutella sinensis fungal powder fermentation manufacturing technique, and its activated protein medium comprises eggs, milk, potato fruit, carrot juice, magnesium sulfate; CN 101423799A discloses the production method of another kind of hirsutella sinensis fungal powder, it is characterized in that being included in the medium that is comprised of cereal, beans, water, and the access bacterial classification is cultivated; Adding the liquid nutrient medium that is comprised of egg, milk, potato, carrot juice, peptone cultivates; Culture is dry, pulverize to get hirsutella sinensis fungal powder.
Put down in writing as above-mentioned two pieces of documents, at present domestic many individuals and unit research and open Chinese caterpillar fungus culture medium and fermentation method for producing, but, these technology adopt microbial fermentation to commonly use medium more, basically with egg, milk, potato as fermentation food source, lack process control in the fermenting and producing, do not adopt the required nutriment of aweto strain spontaneous growth fully.The selection of medium component, although all be according to microbiological the principle of fermentation bacterial classification to be grown, the difference in food source must cause to some extent difference of mycelial character.In addition, the aweto mycelium of producing adopts drying mode dry mostly, because causing the change of mycelium chemical composition and bioactivator, high-temperature drying partly loses, the Cordyceps sinensis fermented product of producing is variant at chemical composition and active substance and natural cordyceps, therefore, fermentation Cordyceps sinensis of the prior art is not thought in academia and pharmaceuticals industry and can be substituted wild cordyceps.
Technology contents
The objective of the invention is to simulate fully with industrial method growing environment and the condition of wild cordyceps, produce and the akin fermented aweto hypha of wild cordyceps and product, the active ingredient of the fermentation mycelium of producing with the inventive method is identical or close with the active ingredient of wild Cordyceps sinensis, can become the substitute of wild cordyceps.
Therefore, the invention provides a kind of Cordyceps sinensis fermentation method for producing, the method comprise Hirsutella sinensis bacterial classification inclined-plane cultivated after the access fermentation tank ferments, wherein cultivate and the used medium that ferments is determined according to the nutrient component of its host bat moth larvae self.
In a specific embodiment, Cordyceps sinensis fermentation method for producing of the present invention, comprise the Hirsutella sinensis bacterial classification is carried out inclined-plane cultivation, shaking flask cultivation, seed tank culture and fermentation tank culture, contain the Yellow meal worm larva powder as culture media nitrogen source in the used medium of wherein inclined-plane cultivation, shaking flask cultivation, seed tank culture and fermentation tank culture, and add vitamin B1 and B2.
Yellow mealworm cries again the bread worm, is under the jurisdiction of coleoptera at entomological taxonomy, Tenebrionidae, and the mealworm first belongs to.The yellow mealworm dry product is fatty 30%, contains protein up to more than 50%, contains in addition macroelement and the various trace elements such as phosphorus, potassium, iron, sodium, aluminium.Contain protein about 40% in the dry Yellow meal worm larva, be described as " protein feeds treasure-house ".A lot of to the research of nutrient component in the Yellow meal worm larva in the prior art, the Yellow meal worm larva powder also is can obtain in the prior art.
The inventor finds that moisture, fat, protein, ash content, amino acid and other trace elements contained in the Yellow meal worm larva powder are near bat moth larvae idiotrophic composition.Because approximate food source can keep the quality of fermented hypha near wild cordyceps.Therefore, the present invention adopts the Yellow meal worm larva powder as the nitrogenous source of Hirsutella sinensis cultivation and medium, and add aweto mycelium grow necessary vitamin B1 and B2, its purpose is exactly to realize that the growth nutriment that obtains and the nutriment that wild cordyceps obtains in pin main body are similar to.
Key of the present invention is to contain the Yellow meal worm larva powder as carbon source and vitamin B1 and B2 in medium.Except the Yellow meal worm larva powder, other compositions in the cultivation of Hirsutella sinensis bacterial classification inclined-plane, shaking flask cultivation, seed tank culture and the fermentation tank culture used medium can to known in the art, same as the prior art or similar, be applicable to carbon source, nitrogenous source composition, trace element and nutriment that Cordyceps sinensis is cultivated.
In a preferred embodiment, the slant medium prescription of Hirsutella sinensis bacterial classification contains Yellow meal worm larva powder, fermented bean drink, glucose, ground rice, agar, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and vitamin B2.The shaking flask culture medium prescription contains Yellow meal worm larva powder, peptone, fermented bean drink, glucose, ground rice, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and B2.Seed tank culture base and fermentation tank culture based formulas contain Yellow meal worm larva powder, ground rice, glucose, fermented bean drink, fish peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and vitamin B2 separately.
The content of Yellow meal worm larva powder in each medium can be 0.5%-2%, preferred 1.0%; Vitamin B1 and the B2 content in each medium can be respectively 0.005-0.02%, preferred 0.01%.Other medium component is not key of the present invention, can be the scope of this area routine, and those skilled in the art can just can determine by simple selection the content of other compositions of medium.
Cordyceps sinensis fermentation method for producing of the present invention, wherein the inclined-plane is cultivated and can be comprised the one-level cultivation, and namely the test tube slant is cultivated; Also can comprise the secondary cultivation, namely the test tube slant is cultivated and the flask cultivation, and the flask cultivation is the inclined-plane cultivation to the further expansion of test tube slant cultivation, so that the shaking flask that more is conducive to is subsequently cultivated.Flask can be selected eggplant-shape bottle (eggplant bottle), and it is the same with general flask, only makes the drainage that eggplant shape is conducive to liquid, and it does not have special purposes, and other flasks also can be used as the inclined-plane culture vessel.Used medium component all was mutually the same during secondary was cultivated.
Cordyceps sinensis fermentation method for producing of the present invention, wherein seed tank culture can comprise the first class seed pot cultivation, also can comprise the cultivation of secondary seed tank, perhaps three grades of seed tank culture.Secondary or three grades of seed tank culture are that the further expansion that first class seed pot is cultivated is cultivated, in order to more be conducive to fermented and cultured subsequently.Used medium component all is mutually the same in secondary or the three grades of cultivations.
No matter be that the inclined-plane is cultivated, or seed tank culture, it all is preferred selecting multistage cultivation, for making the finer and closely woven growth of mycelium, be more suitable in large-scale production, and nonessential process, small-scale production (for example, laboratory cultures) one-level is cultivated just is enough to reach purpose of the present invention.
The process conditions that stages is cultivated comprise temperature, pH value, incubation time etc., all are the selections of this area routine, are that those skilled in the art can be just confirmable by simple selection.
Preferably, the cultivation of stages (comprising inclined-plane cultivation, seed tank culture, fermentation tank culture), its cultivation temperature is 10-20 ℃, and the pH value is pH 6-8; Particularly preferably 15 ℃ ± 2 ℃, pH 7-7.5.Inclined-plane incubation time 30-35 days, each the stage incubation time in shaking flask cultivation, seed tank culture and the fermentation tank culture was 8-10 days.
Chinese caterpillar fungus bacterial filament by fermentation process of the present invention is produced is analyzed with natural cordyceps, can find, natural cordyceps polypide and stroma section color are white, and be little flexible, the little raw meat of gas, mildly bitter flavor.Adopt the inventive method to produce the fermented aweto hypha color and also be white, smell and taste and natural cordyceps have akin little raw meat, little bitterness shape.
Chinese caterpillar fungus bacterial filament to fermentation process production of the present invention, analyze with the chemical composition of natural cordyceps, wherein aminoacid ingredient all approaches with content or is identical, comprise aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, cysteine, methionine, isoleucine, leucine, TYR, aminotoluene acid, lysine, histidine, the content of other nutrient component protein, Cordyceps sinensis polysaccharide, cordycepic acid, cordycepin, adenosine and mannitol also approaches with identical.
In the fermentation process of the present invention, after fermented and cultured finishes, collect mycelia, for example medium centrifugal can be collected, perhaps collect mycelia with the rotating drum vacuum filtration method; Then the mycelia of collecting is carried out drying, such as oven dry or microwave drying etc.; Afterwards ferment cordyceps sinensis mycelia finished product is pulverized, made to the mycelia of drying.
Preferably, the drying of mycelia can adopt the vacuum and low temperature freeze drying, and temperature is controlled at-40～-60 ℃, in order to keep the biologically active of bacterium powder, is difficult for causing variation and the active component forfeiture of the oxidation of bacterium powder and chemical property.
By the Chinese caterpillar fungus bacterial filament product that fermentation process of the present invention is produced, can become the substitute of wild cordyceps product.
Description of drawings
Fig. 1 is Cordyceps sinensis fermentation method for producing process chart of the present invention, has shown the embodiment of the preparation ferment cordyceps sinensis mycelia finished product of a first-selection.
Embodiment
Below in conjunction with accompanying drawing, the concrete industrial processes of a first-selected embodiment of the present invention are described in detail, this embodiment is just in order to illustrate embodiments of the present invention, protection scope of the present invention is not limited to this.Except as otherwise noted, the percentage number average among the present invention refers to percentage by weight.
Embodiment 1
One, preparation medium
1, slant medium prescription:
| The Yellow meal worm larva powder | 1.0％ |
| Fermented bean drink | 1.0％ |
| Glucose | 2.5％ |
| Ground rice | 1.0％ |
| Agar | 1.0％ |
| Potassium dihydrogen phosphate | 0.02％ |
| Magnesium sulfate | 0.01％ |
| Vitamin B1 and vitamin B2 | Each is 0.01% years old |
All the other are water.
2, shaking flask culture medium prescription:
| The Yellow meal worm larva powder | 1.0％ |
| Peptone | 0.2％ |
| Fermented bean drink | 1.0％ |
| Glucose | 3.0％ |
| Ground rice | 1.5％ |
| Potassium dihydrogen phosphate | 0.02％ |
| Magnesium sulfate | 0.01％ |
| Vitamin B1 and B2 | Each is 0.01% years old |
All the other are water.
3, seeding tank and fermentation tank culture based formulas:
| The Yellow meal worm larva powder | 1.0％ |
| Ground rice | 1.5％ |
| Glucose | 3％ |
| Fermented bean drink | 2.0％ |
| The fish peptone | 0.2％ |
| Potassium dihydrogen phosphate | 0.02％ |
| Magnesium sulfate | 0.01％ |
| Vitamin B1 and vitamin B2 | Each is 0.01% years old |
| Defoamer | Trace |
All the other are water.
Each medium is regulated pH=7-7.5.
Two, production method
(1) Yellow meal worm larva powder, ground rice are weighed after, add water by required dosage, then autoclaving leaves standstill a night.Filtered through gauze is got clear liquid, adds glucose, fermented bean drink and KH
2PO
4And MgSO
4, dissolving uses the NaOH adjust pH between the 7-7.5.
Wherein the preparation of fermented bean drink can be: select fresh soyabean or soya bean, the imbibition of make suring that is soaked in water is pulled out and water cleans, and then adds clear water and boils and note moisturizing when 2～5h boils beans, filters, and namely gets fermented bean drink.Every 100g beans can make 5 ° of Be ' fermented bean drink 100mL.
Use prescription and expand the eggplant bottle from the test tube slant to.The cultivation temperature that the inclined-plane is cultivated is 15 ℃ ± 2 ℃, keeps the incubator temperature, opens case every day and ventilates half an hour.Cultivated 30-45 days.Change over to after the eggplant bottle is cultivated in 2-8 ℃ the refrigerator and preserve.
(2) the seed shaking flask is cultivated
With the large triangular flask of 5000ml, it is 1000-1500ml that dress is cultivated base unit weight, each triangular flask inoculation two eggplant bottle (solid), and shaking speed is 120 right sides of turning left.
Preparation: compound method is cultivated with the inclined-plane, and inoculation was shaken 8-10 days, 15 ℃ ± 2 ℃ of cultivation temperature, and at this moment, bacterium liquid turns thick, treats mycelial concentration＞20%, namely can be used as the tank bacterial classification.
(3) seed tank culture
The medium compound method is the same.The canned culture fluid 30-40 of the first order seed of 60 liters liter connects one bottle of the triangular flask of 1500ml loading amount.First class seed pot is cultivated the secondary seed tank that entered 700 liters in 8-10 days, and the amplification quantity of medium is 5-10 times of inoculation liquid.The secondary seed tank is cultivated and was entered three grades of seeding tanks of 2.5 tons in 8-10 days, and the amplification quantity of medium is 5-10 times of inoculation liquid.
Condition of culture: canned amount is below 70%, 15 ℃ ± 2 ℃ of cultivation temperature, mixing speed 110-120r/min, tank pressure 0.065-0.075MPa, air mass flow 0.04-0.06m
3/ h.Incubation time 8-10 days.
(4) fermentation tank culture
The same seeding tank of Medium Proportion.Fermentation culture conditions: cultivation temperature: 15 ℃ ± 2 ℃, tank pressure: 0.04-0.1MPa was controlled at 0.03-0.04m in air mass flow: 1-4 days
3/ h was controlled at 0.05-0.06m in 4-6 days
3/ h was controlled at 0.03-0.05m in 6-10 days
3/ h.Atmospheric density is controlled at every cubic metre of 0.774-0.903 kilogram.Be controlled at 10%, 4-6 days in dissolved oxygen amount: 1-4 days and be controlled at 20%, 6-10 days and be controlled at 15%.
Mixing speed rotating speed: 1-4 days 100-110r/min, 4-6 days 110-120r/min, 6-10 days 90-105r/min.Incubation time: 8-10 days.
Obtain Base in Mycelia of Cordyceps.
Embodiment 2
Process is basically with embodiment 1, just seed tank culture is carried out the secondary cultivation, does not enter three grades of seeding tanks, also adds yeast extract 0.2% in the medium in each stage, and in the fermentation tank culture process, add gradually 0.2% glycerine (sugar alcohol alcohols material).
The tank body that will ferment is sandwich design, passes into the interior temperature of condensed water control tank of different temperatures, realizes that by agitator the zymotic fluid temperature is even in the tank.
Embodiment 3
Process is with embodiment 1, and in incubation, seeding tank sampling in two days detects once, and fermentation tank once a day.Survey the pH value; Survey reducing sugar, use Pei Linfa; Survey nitrogen, survey amino nitrogen with formaldehyde method; Survey concentration, get even sample liquid 10ml, see precipitation quantity with 4000 rev/mins centrifuge after centrifugal 20 minutes, concentration reaches more than 15% for moving the tank standard, can reach 35% in the time of preferably.Survey dry weight, the filter paper of will take a sample numbering oven dry (110 ℃, 60 minutes) is weighed, and measures 10ml sample liquid and pours on the filter paper after weighing in the Buchner funnel.The vacuum drying post-drying is weighed, and it is heavy to deduct paper, is the mycelia dry weight.Dry weight＞0.4% can be put tank.
At this moment, mycelial concentration increases slowly, and the part mycelia has autolysis.Amino nitrogen content drops to about 200 (mg/ml); Reducing sugar drops to about 1-1.5%; The pH value is down to 5.5-5.8.Mycelial concentration 25-35%, corresponding dry weight is 0.4-0.8%.
Embodiment 4
Process is with embodiment 3, after liquid fermentation and culture finishes, open discharging opening, take out culture fluid, collect mycelia with the rotating drum vacuum filtration method, then the mycelia of collecting is carried out the vacuum and low temperature freeze drying, temperature is controlled at-40～-60 ℃, the biologically active that keeps the bacterium powder fully, the drying means of other technologies are oven dry or microwave drying etc., cause easily variation and the active component forfeiture of the oxidation of bacterium powder and chemical property.
Embodiment 5
The fermented hypha that embodiment 4 is made and natural cordyceps outward appearance and composition compare:
(1) outward appearance contrast: Cordyceps sinensis polypide and stroma section color are white, and be little flexible, the little raw meat of gas, mildly bitter flavor.Adopt this method to produce the fermented aweto hypha color and also be white, smell and taste and natural cordyceps have akin little raw meat, little bitterness shape.
(2) chemical composition is relatively:
Measure (indices all differs less than 1%) by this area conventional detection method.
Active ingredient is (indices all differs less than 1%) relatively
| Sample | Natural cordyceps (%) | Fermented cordyceps sinensis mycelia (%) | Difference |
| Protein | 29.60 | 30.00 | 0.4 |
| Cordyceps sinensis polysaccharide | 4.12 | 5.00 | 0.88 |
| Cordycepic acid | 5.49 | 5.03 | 0.46 |
| Cordycepin | 0.0053 | 0.01 | 0.0047 |
| Adenosine | 0.028 | 0.037 | 0.009 |
| Mannitol | 0.07 | 0.08 | 0.01 |
This shows, by the Chinese caterpillar fungus bacterial filament that fermentation process of the present invention is produced, can become the substitute of wild cordyceps.
Claims (9)
1. a Cordyceps sinensis fermentation method for producing is characterized in that, the method comprise Hirsutella sinensis bacterial classification inclined-plane cultivated after the access fermentation tank ferments, wherein cultivate and the used medium that ferments is determined according to the nutrient component of its host bat moth larvae self.
2. according to claim 1 Cordyceps sinensis fermentation method for producing, it is characterized in that, the method comprises carries out inclined-plane cultivation, shaking flask cultivation, seed tank culture and fermentation tank culture with the Hirsutella sinensis bacterial classification, contain the Yellow meal worm larva powder as culture media nitrogen source in the used medium of wherein inclined-plane cultivation, shaking flask cultivation, seed tank culture and fermentation tank culture, and add vitamin B1 and B2.
3. according to claim 2 Cordyceps sinensis fermentation method for producing, it is characterized in that, the slant medium prescription of Hirsutella sinensis bacterial classification contains Yellow meal worm larva powder, fermented bean drink, glucose, ground rice, agar, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and vitamin B2; The shaking flask culture medium prescription contains Yellow meal worm larva powder, peptone, fermented bean drink, glucose, ground rice, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and B2; Seed tank culture base and fermentation tank culture based formulas contain Yellow meal worm larva powder, ground rice, glucose, fermented bean drink, fish peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and vitamin B2 separately.
4. according to claim 2 or 3 Cordyceps sinensis fermentation method for producing, it is characterized in that the inclined-plane is cultivated and comprised that one-level cultivates, namely the test tube slant is cultivated; Perhaps comprise the secondary cultivation, namely the test tube slant is cultivated and the flask cultivation, and used medium component all was mutually the same during secondary was cultivated.
5. according to claim 2,3 or 4 Cordyceps sinensis fermentation method for producing, it is characterized in that seed tank culture comprises the first class seed pot cultivation, perhaps comprise the cultivation of secondary seed tank, or three grades of seed tank culture, used medium component all is mutually the same in secondary or the three grades of cultivations.
6. each Cordyceps sinensis fermentation method for producing is characterized in that according to claim 2-5, and the content of Yellow meal worm larva powder in each medium is 0.5%-2%, preferred 1.0%; Vitamin B1 and the B2 content in each medium is respectively 0.005-0.02%, preferred 0.01%.
7. each Cordyceps sinensis fermentation method for producing according to claim 2-5, the cultivation temperature that each stage cultivates is 10-20 ℃, the pH value is pH 6-8, incubation time is that the inclined-plane was cultivated 30-35 days, and each the stage incubation time in shaking flask cultivation, seed tank culture and the fermentation tank culture is 8-10 days.
8. according to claim 1 and 2 Cordyceps sinensis fermentation method for producing after fermented and cultured finishes, is collected mycelia, then the mycelia of collecting is carried out drying, makes ferment cordyceps sinensis mycelia finished product.
9. according to claim 8 Cordyceps sinensis fermentation method for producing, wherein the drying of mycelia adopts the vacuum and low temperature freeze drying, and temperature is controlled at-40～-60 ℃.
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| CN103444434A (en) * | 2013-08-27 | 2013-12-18 | 王辉 | Fermentation raw material treatment method for improving yield and quality of cordyceps sinensis |
| CN103444435A (en) * | 2013-09-05 | 2013-12-18 | 东方中科生命科学有限责任公司 | Method for breeding high-quality cordyceps sinensis strain |
| CN103483037A (en) * | 2013-09-05 | 2014-01-01 | 东方中科生命科学有限责任公司 | Cordyceps liquid culture medium and application thereof |
| CN103688760A (en) * | 2013-12-20 | 2014-04-02 | 凌中鑫 | Method for culturing artificial cordyceps sinensis by using yellow mealworms as carriers |
| CN104145719A (en) * | 2014-09-04 | 2014-11-19 | 重庆市中药研究院 | Cordyceps sinensis mycelium fermentation production method |
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| CN108739050A (en) * | 2018-05-25 | 2018-11-06 | 广东省生物资源应用研究所 | A kind of aweto fluid nutrient medium and the efficient method for obtaining host of Cordyceps sinensis insect infection blastopore |
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| CN103444434B (en) * | 2013-08-27 | 2015-03-18 | 王辉 | Fermentation raw material treatment method for improving yield and quality of cordyceps sinensis |
| CN103444434A (en) * | 2013-08-27 | 2013-12-18 | 王辉 | Fermentation raw material treatment method for improving yield and quality of cordyceps sinensis |
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| CN112501212A (en) * | 2020-12-22 | 2021-03-16 | 刘随记 | Cordyceps sinensis fermentation process |
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| CN116458647A (en) * | 2023-06-15 | 2023-07-21 | 中国远大集团有限责任公司 | Cordyceps sinensis fermentation composition |
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