CN105878875A - Traditional Chinese medicine ultra-micro powder granules for preventing and treating necrotic enteritis of poultry and preparation method thereof - Google Patents
Traditional Chinese medicine ultra-micro powder granules for preventing and treating necrotic enteritis of poultry and preparation method thereof Download PDFInfo
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Abstract
The invention discloses traditional Chinese medicine ultra-micro powder granules for preventing and treating necrotic enteritis of poultry and a preparation method thereof, and aims to provide a traditional Chinese medicine composition for preventing and treating necrotic enteritis of chickens caused by clostridium welchii. The traditional Chinese medicine ultra-micro powder granules comprise the following components in mass fraction: common andrographis herb, berberis root, purslane, myrobalam, stemona root, dark plum, astragalus root, licorice, medicated leaven and chicory. The components are pure natural traditional Chinese medicinal materials, resources are wide and sufficient, the preparation is easy, and the cost is low. After the components are subjected to ultra-micro grinding and prepared into the granules, the palatability can be increased. After the granules are mixed or taken by drinking water, the medical effects of the traditional Chinese medicines are increased, and the intestinal flora is regulated, so that the granules have a better curative effect on necrotic enteritis of poultry. Compared with currently common medicines, the granules have the advantages of high safety, good curative effect, no residue and the like and guarantee the animal-derived food safety and human body health, so that the granules are good products for treating necrotic enteritis of poultry.
Description
Technical field
The invention belongs to field of veterinary, relate to a kind of Chinese medicine ultra-fine powder granular preparation, a kind of Chinese medicine ultra micro for preventing and treating fowl necrotic enteritis
Powder particles agent and preparation method thereof.
Background technology
Bacillus perfringens, also known as clostridieum welchii, is a kind of Gram-positive anaerobic spore-bearing bacilli, widely exists in the fowl such as soil, water, air
In the surrounding enviroment of class life.Bacillus perfringens is that normal in chicken intestinal can be separated to this antibacterial in flora, the chicken body of any age in days,
Even can also isolated from chicken feather, eggshell.Under normal circumstances, its existence can't be fallen ill among intestinal, but when intestinal environment changes
In the case of change or intestinal suffer damage, normal intestinal flora destroys, and bacillus perfringens will substantial amounts of be bred, and produces the outer poison of clostridieum welchii
Element, makes infection animal be poisoned to death.
The clostridieum welchii of infected poultry mainly belongs to A type, secondly also has c-type.Birds infect clostridieum welchii can occur clinical type disease (NE) and
Subclinical disease.The chickling of clinical type necrotic enteritis (NE) main harm 2-5 week old, Clinical symptoms is acute death and high mortality, gives
Aviculture brings huge economic loss.And Subclinical disease mainly shows as intestinal mucosa focal necrosis, hepatitis or cholangiohepatitis, this will
Affect the processing of poultry product.It addition, clostridieum welchii can also cause human foods transmissible disease and cause other animals to fall ill.
At present vaccine can not effective prevention and control fowl necrotic enteritis, antibacterials preventing and treating is still the Main Means of such disease of control, as sulphuric acid is the most mould
Element, metronidazole, LIJUNJING, gentamycin etc..But, current country limits the use of the medicine such as metronidazole, LIJUNJING, other antibacterials
Due to abuse and incorrect use, the generation of drug resistance has made many antibiotic reduce or lose therapeutical effect, adds the Reusability of overdose
Antibiotic, it is easy to cause the dysbacteriosis of body, resistance declines, and occurs in that multiple antibiotic resistant strain and drug residue problem are more and more prominent.
Therefore, Chinese medicine is low with its residual hazard, can regulate human body immune function and be not likely to produce the advantages such as drug resistance and effect in disease preventing and treating manifests day by day.
Chinese medicine antibacterial, antiinflammatory, heat-clearing and toxic substances removing are probably the treatment more efficiently way of fowl necrotic enteritis.
The various diseases history of existing more than 2000 year of China application Chinese herb prevention people and animals, have accumulated abundant knowledge of TCM and clinical experience.In
Medicine belongs to pure natural substance, compared with antibiotic, chemical synthetic drug, have that toxic and side effects is little, be difficult to residual, free from environmental pollution, drug resistance not
The feature such as substantially.Micronizing is the new and high technology developed rapidly in recent years.Utilize advanced superfine communication technique, develop for animals
Natural drug ultrafine preparation, such preparation compared with Chinese medicine powder, have that effective ingredient rate of release is fast, bioavailability is high, clinical should
With effect is notable, unit kg body weight consumption is few, reduce the features such as cost of material.Chinese medicine can directly be pelletized after micronizing, contributes to developing beast
Granule, capsule, tablet etc., and pelletizing press sheet technique can be simplified.The present invention according to Chinese medical theory and fowl necrotic enteritis pathogenesis etiology,
A kind of prevention that Appropriate application Chinese herbal medicine function cures mainly compatibility and develops and the herbal medicine and preparation method thereof for the treatment of fowl necrotic enteritis.This invention
Medicine is Chinese medicine ultra-fine powder granule, has steady quality, transports, stores, carries, applies the features such as the most more convenient.
Summary of the invention
The present invention is the problems such as drug residue, drug resistance and the drug effect in order to solve antibiotic and chemical synthetic drug may cause be low, it is provided that Yi Zhongneng
Enough pharmaceutical compositions rapid, effectively preventing and treating fowl necrotic enteritis.
Second object of the present invention be in order to overcome Chinese medicine powder consumption greatly, costly, problem that drug effect can not be made to be not fully exerted, carry
For a kind of natural, no drug residue, have no side effect, dose reduces, the eutherapeutic Chinese medicine composition having notable prevention effect to fowl necrotic enteritis
The preparation method of ultrafine powder particle.
Third object of the present invention is to provide the application of a kind of Chinese medicine composition ultrafine powder particle preventing and treating fowl necrotic enteritis.
The present invention is achieved through the following technical solutions: a kind of Chinese medicine ultra-fine powder granule for preventing and treating fowl necrotic enteritis, by following weight portion
Active component is prepared from: Herba Andrographis 80-130 part, Radix Berberidis 50-80 part, Herba Portulacae 50-80 part, Fructus Chebulae's 50-80 part, Radix Stemonae 50-80
Part, Fructus Mume 30-50 part, Radix Astragali 30-50 part, Radix Glycyrrhizae 20-40 part, Massa Medicata Fermentata 20-40 part, Herba Cichorii 20-40 part.
Preferably, the described Chinese medicine ultra-fine powder granule for preventing and treating fowl necrotic enteritis, the active component of following weight portion it is prepared from: wear
Heart lotus 90-120 part, Radix Berberidis 50-70 part, Herba Portulacae 50-70 part, Fructus Chebulae's 50-70 part, Radix Stemonae 50-70 part, Fructus Mume 35-45 part is yellow
Stilbene 35-45 part, Radix Glycyrrhizae 20-30 part, Massa Medicata Fermentata 25-35 part, Herba Cichorii 25-35 part.
A kind of for Chinese medicine ultra-fine powder granule preventing and treating fowl necrotic enteritis and preparation method thereof, step is:
(1) by the various compositions in prescription respectively after primary comminution, in adding super micron mill, it is crushed to 500 mesh sieve percent of pass >=95%, system
Obtain the superfine powder of various components.
(2) each component superfine powder is weighed by weight, Herba Andrographis 90-120 part, Radix Berberidis 50-70 part, Herba Portulacae 50-70 part, Fructus Chebulae 50-70
Part, Radix Stemonae 50-70 part, mix 5-15 minute to uniformly.
(3) each component superfine powder is weighed by weight, Fructus Mume 35-45 part, Radix Astragali 35-45 part, Radix Glycyrrhizae 20-30 part, Massa Medicata Fermentata 25-35 part,
Herba Cichorii 25-35 part, mixes 5-15 minute to uniformly.
(4) above-mentioned (2), (3) step are prepared mixture and be incorporated in mixer, mix 5-15 minute to uniformly；Add Icing Sugar (about to make
The 1/3-1/2 of each amount) and other adjuvant add appropriate 70% ethanol while stirring, after soft material is qualified, by oscillating granulator (12-14 mesh)
Extrusion granulator, after drying, granulate, pack, obtain grain products.
According to monarch in Chinese veterinarian, minister, the principles of formulating prescriptions helping, make, Herba Andrographis has heat clearing away, removing toxic substances, antibacterial, effect of antiinflammatory, to escherichia coli,
The various intestinal bacteria such as Salmonella, clostridieum welchii, dysentery bacterium, necrobacillus and virus all have inhibitory or killing effect, cure mainly bacillary dysentery, enteritis
Deng, for the monarch drug in side.Radix Berberidis, Herba Portulacae are used in conjunction, i.e. clearing away heat-damp and promoting diuresis removing pathogenic heat from blood and toxic substance from the body again, to dysentery bacterium, escherichia coli, Bacillus typhi,
Salmonella, clostridieum welchii etc. have inhibitory action, cure mainly intestinal infection and bloody dysentery, bacillary dysentery etc.；Fructus Chebulae is to various dysentery bacteriums, escherichia coli, wound
Cold bacillus effectively outside, tannin contained by it has astriction to intestinal.The Radix Stemonae has antibacterial, nourishing the lung to arrest cough, insecticidal action；Four medicines share and can help monarch
Medicine heat-clearing and toxic substances removing, antibacterial, dampness-eliminating and dysentery-stopping, be accessory drugs.The adjuvant drugs such as Fructus Mume, the Radix Astragali, Herba Cichorii, Massa Medicata Fermentata be used in combination have astringe the lung, astringing intestine to stop diarrhea,
Antidiarrheal, promote the production of body fluid, invigorating QI to consolidate the body surface resistance, expelling pus and promoting granulation, invigorating the spleen and regulating the stomach, help digestion adjust in, improve immunity, adjust intestinal microbial population effect.Radix Glycyrrhizae conduct
Make medicine, there is invigorating the spleen and replenishing QI and middle emergency, the function of coordinating the actions of various ingredients in a prescription.
Advantage:
The present invention has the advantage that compared to existing technology and the invention provides a kind of Chinese medicine ultra-fine powder granule system for preventing and treating fowl necrotic enteritis
Preparation Method, this Chinese medicine ultra-fine powder granule is to utilize Study on Supermicro-pulverization of Traditional Chinese Drugs technology and add the granule that proper auxiliary materials is made.Micronizing can make medical material
Contained effective ingredient is significantly better than that ordinary powder on stripping quantity and dissolution rate, adds after adjuvant makes granule, also has the advantage that
1, water solublity improves: owing to Chinese medicine particle diameter after micronizing diminishes, swims on the water surface when entering water, is difficult to stir, is unfavorable for moving
Thing waterline is drunk water or gavages administration.Superfine powder after pelletizing, can enter rapidly water and can in the suspension being evenly distributed in water, convenient by drinking-water or
Gavage administration.
2, it is prone to spice use: owing to the little quality of the particle diameter of superfine powder is light, easily dispelled, is not easy to mix all with poultry particulate material conventional at present
Even.Being prone to mix homogeneously with particulate material after pelletizing, therefore superfine powder is made granule and is facilitated spice to use.
3, palatability is improved: all bitter in the mouth such as Herba Andrographis, Radix Berberidis, the Radix Stemonae, effective ingredient stripping quantity also increases while increasing after micronizing
Bitterness, spice or drinking water administration all may result in poultry feed intakes to be reduced.Pelletize after adding Icing Sugar and other auxiliary and condiment, medicine can be improved and fit
Mouth property, does not affect feed intake.
4, it is beneficial to preserve: in prescription, some drugs is after micronizing, increases hygroscopicity because its surface area is big, and some directly come out
Composition may suffer that extraneous factor affects, and as oxidation, volatilization etc. destroy, superfine powder is beneficial to the protection of effective ingredient after making granule.
Therefore this product is made ultrafine powder particle agent by us.
Detailed description of the invention
Being further described the present invention below by specific embodiment, following example are illustrative, are not determinate, it is impossible to limit with this
Determine protection scope of the present invention.
Embodiment 1:
A kind of for Chinese medicine composition ultrafine powder particle agent preventing and treating fowl necrotic enteritis and preparation method thereof, step is:
(1) take the various composition decoction pieces in prescription, be respectively crushed into 100 mesh fine powders, then pulverize through super micron mill, to 500 mesh sieve percent of pass >=95%,
Prepare the superfine powder of various components.
(2) each component superfine powder is weighed by weight, Herba Andrographis 120g, Radix Berberidis 65g, Herba Portulacae 65g, Fructus Chebulae 60g, Radix Stemonae 60g, mix 5-15
Minute to uniformly.
(3) weighing each component superfine powder by weight, Fructus Mume 30g, Radix Astragali 30g, Herba Cichorii 25g, Massa Medicata Fermentata 25g, Radix Glycyrrhizae 20g, mixing 5-15 divides
Clock is to uniformly.
(4) above-mentioned (2), (3) step are prepared mixture and be incorporated in mixer, add Icing Sugar 450g, flavoring agent 50g, mix 5-15 minute extremely
Uniformly, add appropriate 70% ethanol, after soft material is qualified, by oscillating granulator (12-14 mesh) extrusion granulator, after drying, granulate,
Pack, obtains grain products.
Clinical effectiveness excess syndrome:
Example one: the treatment tentative clinical trial of chicken necrotizing enterocolitis
1 materials and methods
1.1 for reagent product
Chinese medicine ultra-fine powder granule of the present invention, it is recommended that dosage 1kg is watered 200kg, or 1kg spice 100kg；Control drug: 6.5% polygynax
Soluble powder.
1.2 experimental animal
450 healthy white plumage broiler chicks of 1 age in days, purchased from certain chicken house of Shenyang, are transported to our company's laboratory pouity dwelling place and raise in cages, and Routine Management feeds
To 10 ages in days, there is not the bacterial diseasees such as escherichia coli through test in laboratory in complete feedstuff, i.e. can be considered that chicken is used in test.Wherein 100 are used for
Doing counteracting toxic substances dosage preliminary experiment, remaining 350 test healthy chicken is divided into 7 groups, and wherein 1,2,3 groups are respectively counteracting toxic substances group, and are medicine of the present invention
High, medium and low dosage treatment group, 4 groups is polygynax soluble powder matched group, and 5 groups is that (health that counteracting toxic substances is not treated is right for negative control group
According to group), 6 groups is positive controls (counteracting toxic substances does not treat matched group), and safe matched group (is used in health according to 5 times of medicine recommended dose of the present invention
Test chicken).
The determination of 1.3 clostridieum welchii artificial challenge's dosage
1.3.1 strain separating is identified
The intestinal contents of aseptic collection clinical disease chicken, is inoculated in 42 DEG C of Anaerobic culturel 24h in anaerobism liver bouillon, then culture is inoculated in purple cattle
42 DEG C of Anaerobic culturel 6h-9h in breast culture fluid, take its anaerobism liver bouillon culture to the sample that purple Lac Bovis seu Bubali explosion fermentation reaction is positive and are inoculated in TSC
42 DEG C of Anaerobic culturel 48h of agar culture medium, choose single doubtful bacterium colony purification and cultivate, take a little isolated strains pure culture smear Gram's staining microscopy.
Take isolated strains pure culture and carry out biochemical test qualification.Extract clostridieum welchii pure culture and the genomic DNA of reference strain of separated qualification,
Carry out PCR qualification.
1.3.2 the preparation of bacterium solution is tested
The clostridieum welchii identified through PCR being inoculated in sterile vegetative meat soup carry out increasing after bacterium, gram stain microscopy is without varied bacteria growing, as test organisms
Liquid, and carry out count plate with plate dilution method and be about 6 × 109CFU/ml。
1.3.3 counteracting toxic substances dosage preliminary experiment
100 healthy chickens, are divided into 5 groups, often group 20.1-4 group is infected group, and every chicken inoculation bacterium solution 0.5ml of each group, bacterium amount is respectively 1.5
×109Individual, 1.2 × 109Individual, 0.9 × 109Individual, 0.6 × 109Individual；5 groups of matched group intramuscular injection 0.5ml normal saline.According to morbidity, pathological changes and cause
Dead situation, finally determines inoculation 2.4 × 109CFU/ml bacterium solution 0.5ml is test counteracting toxic substances amount.
1.4 test method
In addition to negative control group and safe matched group, every chicken intramuscular injection 1.2 × 109Individual clostridieum welchii.Counteracting toxic substances 8 hours, chickling performance lassitude,
Happiness is crouched, is reluctant to stand, stop eating, and major part occurs that dysentery, severe patient feces are mixed with blood, cuts open that the sick chicken small intestinal intestinal swelling of inspection is congested, enteral has bubble
Foam shape liquid, the intestinal mucosa symptom such as have petechia, liver and spleen enlargement hemorrhage.Pathological tissues contact is dyeed, is diagnosed as clostridieum welchii.And horse
Upper medication, uses 5 days continuously, observes therapeutic effect.
Table 1 test packet, drug treating and experiment process
Note: Chinese medicine ultra-fine powder granule crude drug content of the present invention is 50%.
1.5 efficacy determination
Cure: during testing, after for oral administration administration, the chicken mental status, appetite recover normal, and feces preferably and shapes, and bronzing coal tar no longer occurs
The clinical symptoms such as shape loose stool, all belong to healing.
Effective: during testing after for oral administration administration, the chicken cured completely and do not have death but clinical symptom relief person, it is effectively；At the end of experiment,
Often organizing the chicken number of survival and accounting for the percentage rate of test chicken number is effective percentage.
Dead: to occur the classical symptom of chicken clostridieum welchii during testing and still dead after drug treatment for oral administration, necropsy has typical characteristics of lesion,
And go out clostridieum welchii from liver, heart separation and Culture, it is determined that for infecting this bacterium and dead.
Weightening finish: respectively organize chicken body weight during according to on-test and off-test, calculates the average weight gain of often group test chicken.Body weight increase rate is by each medication
Treatment group calculates with the ratio of the weightening finish of normal healthy controls group, and wherein normal healthy controls group chicken rate of body weight gain is set to 100%.
2 result of the tests
After medicine of the present invention and other drug administrations, comparing with infecting matched group, each group medicine all can alleviate more rapidly clinical symptoms.Start to take orally to
After medicine, each group cure rate of chicken, effective percentage, mortality rate and weightening finish are shown in Table 2.
The medicine comparitive study to chicken clostridieum welchii respectively organized by table 2
2.1 cure rates and effective percentage
The medication of each group compares, and medicine of the present invention dosage high, middle group cure rate is higher, all more than 75%；Effective percentage is the highest, all more than 80%.
Low dose group of the present invention, the cure rate of drug control group (polygynax soluble powder), effective percentage are the most relatively low.
2.2 mortality rate
Medicine of the present invention high, medium and low dosage group, and the mortality rate of drug control group is below the mortality rate of positive controls.And medicine of the present invention is high,
Middle dosage group mortality rate is significantly lower than low dose group and drug control group.
2.3 rate of body weight gain
The rate of body weight gain of medicine of the present invention dosage group high, middle is apparently higher than low dose group of the present invention and drug control group.
3 conclusions
3.1 curative effect of medication of the present invention
Medicine high dose group of the present invention and middle dosage group are variant to treatment fowl clostridieum welchii disease curative effect, but inconspicuous, it is contemplated that the economic profit of raiser
Benefit, it is proposed that in medicine of the present invention, dosage i.e. can be applicable to clinical treatment.
3.2 safety testing
During safe matched group medication, the chicken mental status, search for food, feces all normal, cut open internal organs and tissue no abnormality seen, rate of body weight gain after inspection
With negative control group no significant difference, illustrate that medicine of the present invention is safe to target animals chicken.
Example two: curative effect and the impact of ileum flora thereof on clinical infection chicken necrotizing enterocolitis
1 materials and methods
1.1 for reagent product
Chinese medicine ultra-fine powder granule of the present invention, it is recommended that dosage 1kg is watered 200kg, or 1kg spice 100kg；Control drug: Chinese medicine composition of the present invention
The powder (100 mesh fine powders are mixed) of thing, it is recommended that dosage 1kg spice 100kg；6.5% polygynax soluble powder.
1.2 experimental animal
Certain broiler breeding field, area, Liaozhong County, Liaoning Shenyang City, the commercial generation white meat-type chickens of 15 age in days natural infection morbidities, through cuing open inspection and laboratory diagnosis
For chicken necrotizing enterocolitis.
1.3 test method
The isolation of ill chicken at the ill broiler of 0.4-0.5kg, is individually raised by Clinical Selection 330 body weight of this chicken house, feeds complete feed, and with
Machine is divided into 6 groups, often group 55, and feeding environment is identical.Test packet and drug treating see table 1.
Table 1 test packet and drug treating
Note: Chinese medicine ultra-fine powder granule crude drug content of the present invention is 50%.
1.4 testing index and method
1.4.1 weightening finish: the early morning before on-test is administered and during off-test claims each group of chicken body weight before feeding, calculates the average weight gain of often group test chicken.
1.4.2 ileum flora measures
Early morning before on-test is administered and during off-test before feeding often group randomly draw 5 chickens, put to death, open abdominal cavity, ligation contains relatively
The ileum two ends of many feces are also cut, and put into cold preservation in aseptic 20ml centrifuge tube to be measured.Aseptic in aseptic operating platform weigh ileal contents 0.5g,
Adding physiological saline solution 4.5ml, vibrate 3-5min, makes 10-1Diluent, and carry out 10 successively-1～10-7Dilution again.Use pour plate method
To escherichia coli, lactobacillus, bacillus bifidus, clostridieum welchii counting in ileum.Escherichia coli utilize Mai Kangkai culture medium to count, lactobacillus profit
Counting by MRS culture medium, bacillus bifidus utilizes TPY culture medium to count, and bacillus perfringens TSC agar culture medium counts.Converse every gram
Ileal contents clump count represents with log10 (cfu/g) content, and computing formula is as follows: every gram of ileal contents clump count=log10 (bacterium colony
Number × extension rate × every time dilute sample volume)/(inoculation sample volume × sample quality).
1.5 efficacy determination
Cure: during testing, after for oral administration administration, the chicken mental status, appetite recover normal, and feces preferably and shapes, and bronzing coal tar no longer occurs
The clinical symptoms such as shape loose stool, all belong to healing.
Effective: during testing after for oral administration administration, the chicken cured completely and do not have death but clinical symptom relief person, it is effectively；At the end of experiment,
Often organizing the chicken number of survival and accounting for the percentage rate of test chicken number is effective percentage.
Dead: to occur the classical symptom of chicken clostridieum welchii during testing and still dead after drug treatment for oral administration, necropsy has typical characteristics of lesion,
And go out clostridieum welchii from liver, heart separation and Culture, it is determined that for infecting this bacterium and dead.
2 result of the tests
2.1 respectively organize cure rate, effective percentage, mortality rate and weightening finish compares
Comparing with blank group, each group medicine all can alleviate more rapidly clinical symptoms.After administration terminates, medicine high dose group of the present invention and recommendation agent
The cure rate of amount group, effective percentage, weightening finish are above Chinese powder medicine high dose group, recommended dose group and polygynax matched group.Chinese powder medicine height agent
Amount group, recommended dose group cure rate, effective percentage, weightening finish are above polygynax matched group.Refer to table 2.
The comparitive study of medicine respectively organized by table 2
2.2 respectively organize ileum flora compares
Table 3 is respectively organized chicken ileum flora and is compared
Ileum Flora dynamics trend before and after chicken is administered respectively organized by table 4
Respectively group probiotics quantity compares
Medicine of the present invention height, recommended dose group and the lactobacillus of Chinese powder medicine high dose group, bifidobacteria all ratios increase before medication, and remaining is each
Organize the most on a declining curve.Medicine recommended dose group lactobacillus of the present invention, bifidobacteria increase than two groups of Chinese powder medicine and polygynax group
Amplitude is big.
Respectively group harmful bacteria quantity compares
Each medicine group escherichia coli all than reduce before medication, reduce amplitude with medicine high dose group of the present invention bigger.Medicine of the present invention is high, recommended dose
It is relatively big that group clostridieum welchii quantity reduces amplitude, and 4 Chinese drug-treated group are all big than polygynax group clostridieum welchii quantity reduces amplitude.Medicine of the present invention pushes away
Recommend dosage group escherichia coli, that clostridieum welchii quantity reduces amplitude than two groups of Chinese powder medicine and polygynax group is big.
3 conclusions
The fowl that medicine of the present invention is high, recommended dose group is higher than Chinese powder medicine, treatment clostridieum welchii is caused by recommended dose group and polygynax group is downright bad
Property enteritis curative effect is variant, and medicine recommended dose group the most of the present invention i.e. can reach, recommended dose group higher than Chinese powder medicine and polygynax group is more preferable
Curative effect, and for increase intestinal beneficial bacterium quantity, reduce harmful bacteria quantity effect also superior to Chinese powder medicine high dose group, recommended dose group and sulfur
Acid neomycin group.
Medicine recommended dose 1% (containing crude drug 0.5%) spice of the present invention i.e. can reach the powder high dose group (2%) than present invention Chinese medicine, recommended dose
Group (1%) the treatment more preferable effect of fowl necrotic enteritis, illustrates that after Chinese medicine composition of the present invention makes ultrafine powder particle, it reduces by 50% dose and can arrive
Reach drug effect, and cost-saved.
Example three: treatment chicken necrotizing enterocolitis expands clinical trial
1 morbidity chicken public sentiment condition and diagnosis
Certain broiler breeding community, area, Bears high mountain, Liaoning Yingkou City, this Animal Raising Zone has 6 hen houses, about 4000 chickens of every hen house, raises to 13
After age in days carries out fabricius bursa Attenuate vaccine immunity, wherein during 2 hen house 16 ages in days, slowly or material reducing, lassitude, row are thin to occur increasing material successively
Excrement, has mucus, the incidence of the blood streak (block) in feces after element.During 18 age in days, mortality rate suddenly increases to about 2%.It is visible that the chicken that dies of illness cuts open inspection
Small intestinal posterior segment extremely swells, and inside has gas, and intestinal wall is thin and brittle, and outward appearance is shown in that intestinal wall has hemorrhage speckle, intestinal mucosa necrosis to come off, and enteral is full of brown and causes
Close pseudomembrane, and have rufous blood sample thing or the pastel containing blood clot, there is bad smell.Aseptic scraping pathological changes intestinal mucosa position smear, gram
The tubbiness Gram-positive bacillus of the blunt circle in two ends seen from microscopy after dyeing, independent or one-tenth way double-line.According to chicken group's incidence and cut open inspection symptom, in conjunction with
Laboratory diagnosis, meets the symptom that chicken necrotizing enterocolitis infects.
2 test methods
The most repeatedly there is this type of disease in the past in this plant, uses antibiotics to treat traditionally, can control to a certain extent
Dead continues to increase, but has produced drug resistance and drug residue, and later stage death rate is bigger.Through with this factory technical staff's communication and consultation after, right
The hen house of morbidity carries out curative effect contrast test.Wherein, a hen house uses the most used conventional polygynax soluble powder as a control group；Another
Hen house, is randomly divided into two experimental grouies, uses medicine ultrafine powder particle of the present invention, the powder of Chinese medicine composition of the present invention respectively.Experimental group is with right
According to the mortality rate in group all one course for the treatment of (5 days) of statistics.
3 result of the tests:
Note: Chinese medicine ultra-fine powder granule crude drug content of the present invention is 50%.
As a result, the polygynax group feces morning on the 4th starts to improve, and mortality rate has declined, medication always to the 5th day, the most ill chicken of hen house
The phenomena of mortality.Medicine ultrafine powder particle group of the present invention feces afternoon on the 2nd starts to improve, and after medication the 4th day, occurs the phenomena of mortality the most again.
Drug powder of the present invention feces afternoon on the 3rd starts to improve, and after medication the 5th day, indivedual chicken phenomena of mortality occurs.Result above shows, this
Bright medicine ultrafine powder particle is better than drug powder group of the present invention and antibiotic group to the therapeutic effect of chicken necrotizing enterocolitis.
The requirement of Hou Ying plant, polygynax group also uses medicine ultrafine powder particle of the present invention treatment, effectively controls death after being used in conjunction 2 days.
Other hen houses prevent with medicine ultrafine powder particle spice of the present invention, and dosage halves, 0.5% spice, are used in conjunction 5 days, do not occur before delivering for sale to 45 ages in days
This type of disease, demonstrates the effect of this Chinese medicine further.
By above-mentioned 2 Clinic Cases, compared with Chinese medicine powder, the present invention is in the case of preventing and treating fowl necrotic enteritis dosage reduces 50%
Can reach more preferable therapeutic effect, and in use without any untoward reaction.Test prove the present invention medicine the most safely, have
Effect, noresidue, especially more preferable to adjusting intestinal microbial population effect, and dosage minimizing, reduce cost, there is preferable economic benefit and social benefit.
Claims (2)
1., for preventing and treating a Chinese medicine ultra-fine powder granule for fowl necrotic enteritis, it is characterized in that being formed by following components mixed preparing by weight: wear
Heart lotus 80-130 part, Radix Berberidis 50-80 part, Herba Portulacae 50-80 part, Fructus Chebulae's 50-80 part, Radix Stemonae 50-80 part, Fructus Mume 30-50 part,
Radix Astragali 30-50 part, Radix Glycyrrhizae 20-40 part, Massa Medicata Fermentata 20-40 part, Herba Cichorii 20-40 part.Preparation method is: by above-mentioned raw materials through superfine powder
Granular preparation it is mixed and made into after broken.
2. the Chinese medicine ultra-fine powder granular preparation being used for preventing and treating fowl necrotic enteritis, it is characterised in that: it is for preventing and treating fowl necrotic enteritis
Disease.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410775060.0A CN105878875A (en) | 2014-12-15 | 2014-12-15 | Traditional Chinese medicine ultra-micro powder granules for preventing and treating necrotic enteritis of poultry and preparation method thereof |
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| CN113116956A (en) * | 2021-04-14 | 2021-07-16 | 爱迪森(北京)生物科技有限公司 | Traditional Chinese medicine premix and preparation method and application thereof |
| CN113317273A (en) * | 2021-06-11 | 2021-08-31 | 四川省畜牧科学研究院 | Construction method of bacterial enteritis model of young rabbits |
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| CN113116956A (en) * | 2021-04-14 | 2021-07-16 | 爱迪森(北京)生物科技有限公司 | Traditional Chinese medicine premix and preparation method and application thereof |
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