CN105858894A - Method for nitrogen abundance transformation treatment of wastewater with high ammonia nitrogen - Google Patents

Method for nitrogen abundance transformation treatment of wastewater with high ammonia nitrogen Download PDF

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CN105858894A
CN105858894A CN201610095104.4A CN201610095104A CN105858894A CN 105858894 A CN105858894 A CN 105858894A CN 201610095104 A CN201610095104 A CN 201610095104A CN 105858894 A CN105858894 A CN 105858894A
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周文广
阮榕生
王菁晗
黎俊
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Nanchang University
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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Abstract

The invention discloses a method for nitrogen abundance transformation treatment of wastewater with high ammonia nitrogen by utilizing mixotrophic microorganisms; with use of microbial cells, such as microalgae, photobacteria and other chemical energy bacteria, obtained from pre-culture or harvested from a culture system, mixotrophic microbial cells are subjected to rapid culture accumulation in an abundant nitrogen culture medium, and followed by mixotrophic microorganisms are harvested and are transferred to a nitrogen limited culture medium, are subjected to nitrogen limited culture for 2-4 days, finally are transferred into the wastewater with high ammonia nitrogen and are subjected to autotrophic growth under illumination conditions; because the mixotrophic microorganisms need to synthesize liposome for saving energy in the nitrogen limited process, and simultaneously degrade chloroplast and other organelles, in the abundant nitrogen transformation process under the illumination conditions, a large number of ammonia nitrogen nutrient sources are needed for synthesizing chlorophylls and other assemblies needed for photosynthesis, and thus the high-concentration ammonia nitrogen in the wastewater can be effectively removed, and the purpose of purifying the wastewater is achieved.

Description

A kind of method of nitrogen abundance conversion process high ammonia-nitrogen wastewater
Technical field
The present invention relates to technical field of environmental management, particularly to a kind of method utilizing mixotroph nitrogen abundance conversion process high ammonia-nitrogen wastewater, preculture is used to obtain the most exactly or the microbial cell such as microalgae of results from cultivating system, photobacteria and other change energy antibacterials etc., High Density Cultivation accumulation mixotroph cell is carried out in rich nitrogen culture medium, then gather in the crops this mixotroph and proceed to limit nitrogen culture medium carries out limiting nitrogen inducing culture 2-4 days, finally proceed to carry out in high ammonia-nitrogen wastewater and under illumination condition autophyting growth, thus efficiently remove the process of the high ammonia nitrogen in waste water.
Background technology
It is worldwide a great problem that high ammonia-nitrogen wastewater purifies, and traditional activated sludge process removes ammonia nitrogen by nitrification-denitrification, generally needs additional organic carbon source because of carbon source deficiency, and aerator construction operation, adding of organic carbon source all add cost for wastewater treatment.Ammonia nitrogen is the nitrogen source form that microalgae the most easily utilizes, and when microalgae is by photosynthesis autophyting growth, absorbs ammonia nitrogen and is not required to organic carbon source without aeration, with the obvious advantage compared with activated sludge process.Raising microalgae yield can reduce bio-fuel and produce cost；Improve microalgae inoculum density and can improve anti-shock loading and the ammonia nitrogen removal effect of sewage purification system.Autotrophy, hold concurrently support, heterotrophism is three kinds of Model of Nutritions of microalgae, most of microalgae can only autotrophy, part can be held concurrently and support and heterotrophism.Compared with tradition autotrophy, heterotrophism, double supporting generally are remarkably improved microalgae yield, have Great significance.
Nitrogen is the important element affecting micro algae growth, and during nitrogen hunger, in microalgae, the synthesis of nitrogen-containing compound such as protein, nucleic acid, chlorophyll etc. is suppressed, and growth slows down even stagnates, and carbon metablism approach is turned to lipid or Starch synthesis by protein synthesis simultaneously.
Nitrogen is the important nutrient affecting micro algae growth, generally speaking, under the conditions of nitrogen hunger, the synthesis of the nitrogen-containing compound needed for microalgae cell growth such as protein, nucleic acid, chlorophyll etc. is suppressed, and these materials grow for microalgae cell, division is necessary, cause micro algae growth to significantly slow and even stagnate.Meanwhile, the metabolic pathway of carbon is turned to lipid or Starch synthesis by protein synthesis, to lay in carbon source and energy.But, conventional research all concentrates on for the purpose of preparing bio-fuel about nitrogen hunger micro algae growth and oils and fats or the impact of starch accumulation.High crop and marine phytoplankton are recovered to rich nitrogen by nitrogen hunger, the tendency of more nitrogen is absorbed when having than the richest nitrogen, but study growth and the change of cell composition being concerned only with microalgae after nitrogen hunger, the growth of microalgae after returning to rich nitrogen from nitrogen hunger and nitrogen element absorption ability have but been rarely had and relates to.
Summary of the invention
It is an object of the invention to provide a kind of method utilizing mixotroph nitrogen abundance conversion process high ammonia-nitrogen wastewater.Owing to mixotroph is cultivated turn rich nitrogen incubation from limit nitrogen, need substantial amounts of ammonia nitrogen nutrient source synthesis chlorophyll and the assembly of other photosynthesis needs thus develop a kind of efficient process for treating high ammonia nitrogen waste water the most efficient, with low cost.
Mixotroph in the present invention belongs to can carry out the microorganism that photosynthesis can be able to act in carrying out under dark condition again, volume is small under illumination condition, owing to mixotroph needs synthetic fat plastid for preserving energy during limit nitrogen, the degraded organelle such as chloroplast simultaneously, therefore during turning rich nitrogen under illumination condition, need substantial amounts of ammonia nitrogen nutrient source for synthesizing the assembly of chlorophyll and other photosynthesis needs, it is thus possible to efficiently remove the ammonia nitrogen in high density in waste water, reach to purify the purpose of waste water.
The present invention adopts the following technical scheme that
The method of the nitrogen abundance conversion process high ammonia-nitrogen wastewater of the present invention is to use preculture to obtain or the mixotroph cell of results from cultivating system, join in rich nitrogen culture medium and carry out High Density Cultivation, then gather in the crops mixotroph cell and proceed to limit inducing culture 2-4 days in nitrogen culture medium, the cell of results proceeds in high ammonia-nitrogen wastewater again, and under illumination condition, carry out autophyting growth, thus remove the ammonia nitrogen in high density in waste water, arrive the purpose purifying waste water.
Described mixotroph is to utilize photosynthesis and inorganic carbon source to carry out autophyting growth, organic carbon source can be utilized to carry out heterotrophic growth again under dark condition, moreover it is possible to utilize the inorganic microorganism carrying out double health preserving length with organic carbon source under illumination condition simultaneously.
Described mixotroph is microalgae or photosynthetic bacteria.
Described microalgae is Chlorella (Chlorella sp.), barrel mast Trentepohlia (Cylindrotheca sp.), diatom (Diatom), rhombus algae (Nitzschia sp.), split kettle algae (Schizochytrium sp.), Dunaliella (Dunaliella), scenedesmus (Scenedesmus sp.), Nannochloropsis oceanica (Nannochloris sp.), Chlamydomonas (Chlamydomonas sp.), flat algae (Tetraselmis sp.) or empty ball Trentepohlia (Eudorina sp.)；Described photosynthetic bacteria is cyanobacteria (Cyanobacteria)；Described cyanobacteria (Cyanobacteria) is that Synechococcus belongs to (Synechococcus), quivers blue Pseudomonas (Oscillatoria), purple bacteria such as Rhodospirillum (Rhodospirillum), Rhodopseudomonas (Rhodopseudomonas) or Rhodomicrobium (Rhodomicrobium) greatly.
Described rich nitrogen culture medium is the nitrogen content culture medium at 0-4000mg/L；Described rich nitrogen culture medium is synthetic medium or rich nitrogen waste water；Described synthetic medium includes autotrophy culture medium, raises together with culture medium and Heterotrophic culture base, described in raise together with the organic carbon source concentration initial reduction sugar concentration range with Heterotrophic culture base be 0.01-200g.L-1.Described synthetic medium is preferably BG-11 culture medium, F/2 culture medium, walne culture medium or TAP culture medium.
Described high ammonia-nitrogen wastewater is municipal wastewater, industrial and agricultural wastewater, food wastewater, sanitary wastewater, waste water of paper mill, molasses containing waste water, beer brewery water, dairy food processing plants waste water, biogas fermentation waste water or animal wastes waste water.
The pH scope of described High Density Cultivation and inducing culture is 0.1-10；The temperature range of described cultivation is 4-45 DEG C.
Specifically comprising the following steps that of the method for the nitrogen abundance conversion process high ammonia-nitrogen wastewater of the present invention
(1) switching of mixotroph:
From-70 DEG C of refrigerators take out frozen mixotroph bacterial strains and with inoculating loop scraping a little to solid slope flat board illumination autotrophy, raise together with or Heterotrophic culture；
Described autotrophy condition of culture is as follows: temperature controls in the range of 20-45 DEG C, is optimal with 28 DEG C；The illumination cultivation nitrogen source such as initial concentration such as glycine, yeast extract between 1-15g.L-1, preferably 4g.L-1, be passed through air or air and CO2Mixed gas, ventilation 50-300L/h, preferably 80-120L/h；CO2Concentration 0.9-3%, uses 10-200 μm ol.m in incubation-2s-1Sun exposure, pH value controls, between 5-9, to be preferred with 7.0；Depending on total incubation time visual cell growing state, it is typically in the range of 50-400 hour, preferably 120-200 hour；
Described raising together with is as follows with Heterotrophic culture condition: organic carbon source such as concentration of glucose 0.01-200g.L-1, preferably 20g.L-1；It is passed through air, ventilation 100-400L/h, preferably 150-250L/h；Incubation uses 5-40 μm ol.m-2s-1Sun exposure, pH value controls, between 5-9, to be preferred with 7.0；Depending on total incubation time visual cell growing state, it is typically in the range of 72-200 hour, preferably 100-150 hour；
(2) mixotroph rich in organic carbon rich nitrogen waste water or autotrophy, raise together with and Heterotrophic culture base middle-high density Heterotrophic culture:
Plate culture is seeded to bioreactor culture, rich in the waste water of organic carbon or raise together with and Heterotrophic culture base middle-high density Heterotrophic culture；Biological reaction apparatus includes shaking flask, ventilation bottle, bioreactor, fermentation tank or open culturing pond；Until cell log trophophase, cell density reaches 106-1010Above；
Described raising together with is as follows with Heterotrophic culture condition: organic carbon source such as concentration of glucose 0.01-200g.L-1, preferably 20g.L-1；It is passed through air, ventilation 100-400L/h, preferably 150-250L/h；Incubation uses 5-40 μm ol.m-2s-1Sun exposure, pH value controls, between 5-9, to be preferred with 7.0；Depending on total incubation time visual cell growing state, it is typically in the range of 72-200 hour, preferably 100-150 hour；
(3) results of mixotroph cell:
Take the logarithm the heterotrophic cell of Later growth or stable phase, the slow-speed of revolution (800-2000g) harvested by centrifugation, the mixotroph cell of results aseptic water washing twice, the most again gathers in the crops；(4) the mixotroph cell gathered in the crops be transferred to limit nitrogen culture medium carry out limit nitrogen cultivate:
The mixotroph cell of results adds limit nitrogen culture medium in the ratio of 1:1 to 1:100 to carry out limiting nitrogen inducing culture 2-4 days；
Described limit nitrogen inducing culturing condition is as follows: temperature controls in the range of 20-45 DEG C, is optimal with 28 DEG C；Illumination cultivation nitrogen source is ammonia nitrogen, and its initial concentration is between 40-160mg.L-1, preferably 80mg.L-1, it is passed through air or air and CO2Mixed gas, ventilation 50-300L/h, preferably 80-120L/h；CO2Concentration 0.1-15%, uses 10-200 μm ol.m in incubation-2s-1Sun exposure, pH value controls, between 5-9, to be preferred with 7.0；Depending on total incubation time visual cell growing state, it is typically in the range of 4-400 hour, preferably 12-48 hour；
(5) limit the microalgae cell efficient absorption height ammonia nitrogen nutrient of nitrogen cultivation and purify waste water:
The limit nitrogen cell of results proceeds to high ammonia-nitrogen wastewater cultivate, absorb ammonia nitrogen in high density and purify waste water.
The positive effect of the present invention is as follows:
The present invention is by nitrogen abundance Changing Strategy, owing to mixotroph turns autotrophy rich nitrogen incubation from limit nitrogen inducing culture, need substantial amounts of ammonia nitrogen synthesis chlorophyll and the assembly of other photosynthesis needs thus develop a kind of biological high-efficiency the most efficient, with low cost and process high-concentration ammonia nitrogenous wastewater method.This technique can be used to meet heavy industrialization waste water further and processes and be substantially reduced cost.
The beneficial effects of the present invention is and invention introduces the method changing nitrogen abundance Nutrition and Metabolism approach, be used for processing high ammonia-nitrogen wastewater.The waste water processed can recycle again, meets microalgae industryization and processes the requirement of wastewater application, is an economy, the new way of microalgae sewage disposal the most processed.The microalgae cell of results can process the preparation for bioenergy and animal feed etc. further.
Accompanying drawing explanation
Fig. 1 is that chlorella (Cholorella sorokiniana) is in autotrophy, heterotrophism, the growth characteristics supported under (raising together with) of holding concurrently.
Fig. 1 a represents chlorella Cholorella sorokiniana and in autotrophy and raises together with growth curve chart in culture medium, and P represents autotrophy and cultivates, and M represents and raises together with cultivation, and M1-M50 represents the concentration of organic carbon when raising together with cultivation.From Fig. 1 a it can be seen that raise together with cellular biomass in the identical time to be significantly larger than autotrophy culture biomass.And organic carbon concentration is directly proportional to raising together with Biomass；
Fig. 1 b represents chlorella Cholorella sorokiniana growth curve chart in Heterotrophic culture base.Wherein H represents Heterotrophic culture, the concentration of organic carbon when H1-H50 represents Heterotrophic culture.From Fig. 1 b it can be seen that in the identical time autotrophic cell Biomass be significantly larger than autotrophy culture biomass, suitable with raising together with Biomass.And organic carbon concentration is directly proportional to autotroph amount；Therefore use to raise together with and test for next step with heterotrophism High Density Cultivation very Rapid Accumulation micro algae biomass.
Fig. 2 chlorella (Cholorella sorokiniana) autotrophy, heterotrophism, hold concurrently support under rich nitrogen and limit nitrogen cell transmission electron microscope picture；
A () original seed autotrophy NS, M group, wherein NS, M represents rich nitrogen and raises together with cultivation, and original seed autotrophy refers to that when initial incubation microalgae be autotrophy culture medium；B () original seed autotrophy NS, H group, wherein NS, H represents rich nitrogen Heterotrophic culture, and original seed autotrophy refers to that when initial incubation microalgae be autotrophy culture medium；C () original seed heterotrophism NS, M group, wherein NS, M represents rich nitrogen and raises together with cultivation, and original seed heterotrophism refers to that when initial incubation microalgae be Heterotrophic culture base；；D () original seed heterotrophism NS, H group, wherein NS, H represents rich nitrogen Heterotrophic culture, and original seed heterotrophism is supported and referred to that when initial incubation microalgae be Heterotrophic culture base；.Figure it is seen that in autotrophy incubation, it can be observed that chloroplast, lipid granule is the least and inconspicuous, is raising together with in Heterotrophic culture, and chloroplast disappears, and lipid granule is obvious, and it is observed that starch granules.Illustrate to use to raise together with and accumulate cell with Heterotrophic culture and use the method for limit nitrogen induction can quickly remove high ammonia-nitrogen wastewater.
NH in Fig. 3 nitrogen abundance conversion process4+The change of-N concentration；
A () raises together with and middle NH in heterotrophism high density and limit nitrogen incubation4+The change of-N concentration.Wherein NS, M are that rich nitrogen raises together with High Density Cultivation；NS, H are rich nitrogen heterotrophism High Density Cultivation；ND, the M nitrogen that is limited raises together with cultivation；ND, H are limited nitrogen Heterotrophic culture；(b) high NH4+The change of-N waste strength, initial NH4+-N concentration 80mg/L；(c) high NH4+The change of-N waste strength, initial NH4+-N concentration 160mg/L.From figure 3, it can be seen that use the nitrogen rich method changed ammonia nitrogen removal frank in initial concentration is into the ammonia nitrogen waste water of 80mg/L of the present invention to reach as high as 12.4mg/L/d；In the ammonia nitrogen waste water that initial concentration is 160mg/L, ammonia nitrogen removal frank reaches as high as 19.1mg/L/d.
Detailed description of the invention
The following examples are that the present invention is described in further detail.
Embodiment 1
The local screening of high ammonia nitrogen tolerance algae chlorella (Cholorella sorokiniana), this algae strain can be at photosynthetic autophyting growth, again can be at heterotrophic growth under dark condition, moreover it is possible to mixture growth the most under these conditions.Its autotrophy, heterotrophism and the growth characteristics under the conditions of raising together with are as shown in Figure 1.
Wherein autotrophy culture medium prescription is: K2HPO4·3H2O 0.04g/L, MgSO4·7H2O 0.075g/L, CaCl2·2H2O 0.036g/L, citric acid 0.006g/L, ferric ammonium citrate 0.006g/L, EDTA 0.001g/L, NaNO31.5g/L, Na2CO30.02g/L, A5 liquid microelement 1.5ml/L, wherein A5 liquid microelement composition: H3BO32.86g/L, MnCl2·4H2O 1.81g/L, ZnSO4·7H2O 0.222g/L, NaMoO4·2H2O 0.39g/L, CuSO4·5H2O 0.079g/L, and CoCl2·6H2O 0.05g/L. wherein NH4The concentration of Cl is 40mg/L, 80mg/L and 160mg/L.
Ibid, and to add different organic carbon source be 0.1-200g.L-1 to initial reduction sugar concentration to Heterotrophic culture based formulas, optimum 15g.L-1.
Raising together with culture medium prescription ibid, and add different organic carbon sources and inorganic carbon source, organic carbon source is 0.1-200g.L to initial reduction sugar concentration-1, optimum 15g.L-1.Inorganic carbon source is 0.001%-100%CO2, optimum 2%, or DIC NaHCO3Or Na2CO3Or both mixing, concentration is 0.1-100g.L-1, optimum 0.2g.L-1。
High ammonia-nitrogen wastewater formula is as follows: NaCl 0.007g/L, MgSO4·7H2O 0.002g/L, CaCl2·2H2O 0.004g/L, KH2PO40.0085g/L, K2HPO40.0217g/L, Na2HPO40.025g/L, Trace Metal solution 0.1ml/L, wherein A5 Trace Metal solution composition: H3BO35.7g/L, MnCl2·4H2O 2.5g/L, ZnSO4 7H2O 11g/L, FeSO4·7H2O 2.5g/L, Na2MoO4·2H2O 0.15g/L, Na2EDTA 25g/L, and CoCl2·6H2O 0.8g/L.
Finally regulation adds different amounts of NH4The concentration of Cl regulation culture medium ammonia nitrogen, makes the concentration of ammonia nitrogen at 80mg/L.
By the method described in step 1, inoculating during chlorella cells is cultivated to solid plate and activate algae strain, then cultivated to shaking table in 250mL shaking flask by the chlorella list colony inoculation being grown on solid medium in-70 DEG C of refrigerators, temperature controls at 28 DEG C ± 5 DEG C；After cell enters logarithmic growth latter stage, access rich nitrogen and in biological reaction apparatus, carry out high density autotrophy, heterotrophism rich in organic carbon waste water or synthetic medium and raise together with cultivation.Biological reaction apparatus includes shaking flask, ventilation bottle, various bioreactor, fermentation tank and open culturing pond etc..Cultivating at above-mentioned suitable condition, until cell log trophophase, cell density reaches (106-1010Above).Finally take the logarithm the microalgae cell of Later growth or stable phase, the slow-speed of revolution (＜ 8000r/min) harvested by centrifugation, the mixotroph cell of results aseptic water washing twice.The mixotroph cell of results adds limit nitrogen culture medium according to a certain percentage to carry out limiting nitrogen inducing culture 2-4 days, then gather in the crops, proceed to high ammonia-nitrogen wastewater is carried out autotrophy cultivation again, result display uses the method utilizing mixotroph rich nitrogen conversion process high ammonia-nitrogen wastewater of the present invention, and in the high ammonia-nitrogen wastewater that initial concentration is 80mg/L, ammonia nitrogen removal frank reaches as high as 12.4mg/L/d (as shown in Figure 3 c).
Embodiment 2
The high ammonia-nitrogen wastewater that Nanchang municipal wastewater treatment plant collects, its total nitrogen concentration is at 178mg/L, and ammonia nitrogen concentration is 160mg/L.Carry out successively initially with above method High Density Cultivation, gather in the crops, limit nitrogen cultivate and harvesting microalgae cell, proceed to high ammonia-nitrogen wastewater is carried out autotrophy cultivation the most again, result display uses the method utilizing mixotroph rich nitrogen conversion process high ammonia-nitrogen wastewater of the present invention, and in the high ammonia-nitrogen wastewater that initial concentration is 160mg/L, ammonia nitrogen removal frank reaches as high as 19.1mg/L/d (as shown in Figure 3 c).
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, being appreciated that and these embodiments can carry out multiple change without departing from the principles and spirit of the present invention, revise, replace and modification, the scope of the present invention be defined by the appended.

Claims (9)

1. the method for a nitrogen abundance conversion process high ammonia-nitrogen wastewater, it is characterized in that: described method is to use preculture to obtain or the mixotroph cell of results from cultivating system, join in rich nitrogen culture medium and carry out High Density Cultivation, then gather in the crops mixotroph cell and proceed to limit inducing culture 2-4 days in nitrogen culture medium, the cell of results proceeds in high ammonia-nitrogen wastewater again, and under illumination condition, carry out autophyting growth, thus remove the ammonia nitrogen in high density in waste water, arrive the purpose purifying waste water.
2. the method for nitrogen abundance conversion process high ammonia-nitrogen wastewater as claimed in claim 1, it is characterized in that: described mixotroph is to utilize photosynthesis and inorganic carbon source to carry out autophyting growth, organic carbon source can be utilized under dark condition to carry out heterotrophic growth again, moreover it is possible to utilize the inorganic microorganism carrying out double health preserving length with organic carbon source under illumination condition simultaneously.
3. the method for nitrogen abundance conversion process high ammonia-nitrogen wastewater as claimed in claim 1, it is characterised in that: described mixotroph is microalgae or photosynthetic bacteria.
null4. the method for nitrogen abundance conversion process high ammonia-nitrogen wastewater as claimed in claim 3，It is characterized in that: described microalgae is Chlorella (Chlorella sp.)、Barrel mast Trentepohlia (Cylindrotheca sp.)、Diatom (Diatom)、Rhombus algae (Nitzschia sp.)、Split kettle algae (Schizochytrium sp.)、Dunaliella (Dunaliella)、Scenedesmus (Scenedesmus sp.)、Nannochloropsis oceanica (Nannochloris sp.)、Chlamydomonas (Chlamydomonas sp.)、Flat algae (Tetraselmis sp.) or empty ball Trentepohlia (Eudorina sp.)；Described photosynthetic bacteria is cyanobacteria (Cyanobacteria)；Described cyanobacteria (Cyanobacteria) is that Synechococcus belongs to (Synechococcus), quivers blue Pseudomonas (Oscillatoria), purple bacteria such as Rhodospirillum (Rhodospirillum), Rhodopseudomonas (Rhodopseudomonas) or Rhodomicrobium (Rhodomicrobium) greatly.
5. the method for nitrogen abundance conversion process high ammonia-nitrogen wastewater as claimed in claim 1, it is characterised in that: described rich nitrogen culture medium is the nitrogen content culture medium at 0-4000mg/L；Described rich nitrogen culture medium is synthetic medium or rich nitrogen waste water；Described synthetic medium includes autotrophy culture medium, raises together with culture medium and Heterotrophic culture base, described in raise together with the organic carbon source concentration initial reduction sugar concentration range with Heterotrophic culture base be 0.01-200g.L-1。
6. the method for nitrogen abundance conversion process high ammonia-nitrogen wastewater as claimed in claim 5, it is characterised in that: described synthetic medium is BG-11 culture medium, F/2 culture medium, walne culture medium or TAP culture medium.
7. the method for nitrogen abundance conversion process high ammonia-nitrogen wastewater as claimed in claim 1, it is characterized in that: described high ammonia-nitrogen wastewater is municipal wastewater, industrial and agricultural wastewater, food wastewater, sanitary wastewater, waste water of paper mill, molasses containing waste water, beer brewery water, dairy food processing plants waste water, biogas fermentation waste water or animal wastes waste water.
8. the method for nitrogen abundance conversion process high ammonia-nitrogen wastewater as claimed in claim 1, it is characterised in that: the pH scope of described High Density Cultivation and inducing culture is 0.1-10；The temperature range of described cultivation is 4-45 DEG C.
9. the method for nitrogen abundance conversion process high ammonia-nitrogen wastewater as claimed in claim 1, it is characterised in that: specifically comprising the following steps that of described method
(1) switching of mixotroph:
From-70 DEG C of refrigerators take out frozen mixotroph bacterial strains and with inoculating loop scraping a little to solid slope flat board illumination autotrophy, raise together with or Heterotrophic culture；
Described autotrophy condition of culture is as follows: temperature controls in the range of 20-45 DEG C, is optimal with 28 DEG C；The illumination cultivation nitrogen source such as initial concentration such as glycine, yeast extract between 1-15g.L-1, preferably 4g.L-1, be passed through air or air and CO2Mixed gas, ventilation 50-300L/h, preferably 80-120L/h；CO2Concentration 0.9-3%, uses 10-200 μm ol.m in incubation-2s-1Sun exposure, pH value controls, between 5-9, to be preferred with 7.0；Depending on total incubation time visual cell growing state, it is typically in the range of 50-400 hour, preferably 120-200 hour；
Described raising together with is as follows with Heterotrophic culture condition: organic carbon source such as concentration of glucose 0.01-200g.L-1, preferably 20g.L-1；It is passed through air, ventilation 100-400L/h, preferably 150-250L/h；Incubation uses 5-40 μm ol.m-2s-1Sun exposure, pH value controls, between 5-9, to be preferred with 7.0；Depending on total incubation time visual cell growing state, it is typically in the range of 72-200 hour, preferably 100-150 hour；
(2) mixotroph rich in organic carbon rich nitrogen waste water or autotrophy, raise together with and Heterotrophic culture base middle-high density Heterotrophic culture:
Plate culture is seeded to bioreactor culture, rich in the waste water of organic carbon or raise together with and Heterotrophic culture base middle-high density Heterotrophic culture；Biological reaction apparatus includes shaking flask, ventilation bottle, bioreactor, fermentation tank or open culturing pond；Until cell log trophophase, cell density reaches 106-1010Above；
Described raising together with is as follows with Heterotrophic culture condition: organic carbon source such as concentration of glucose 0.01-200g.L-1, preferably 20g.L-1；It is passed through air, ventilation 100-400L/h, preferably 150-250L/h；Incubation uses 5-40 μm ol.m-2s-1Sun exposure, pH value controls, between 5-9, to be preferred with 7.0；Depending on total incubation time visual cell growing state, it is typically in the range of 72-200 hour, preferably 100-150 hour；
(3) results of mixotroph cell:
Take the logarithm the heterotrophic cell of Later growth or stable phase, the slow-speed of revolution (800-2000g) harvested by centrifugation, the mixotroph cell of results aseptic water washing twice, the most again gathers in the crops；
(4) the mixotroph cell gathered in the crops be transferred to limit nitrogen culture medium carry out limit nitrogen cultivate:
The mixotroph cell of results adds limit nitrogen culture medium in the ratio of 1:1 to 1:100 to carry out limiting nitrogen inducing culture 2-4 days；
Described limit nitrogen inducing culturing condition is as follows: temperature controls in the range of 20-45 DEG C, is optimal with 28 DEG C；Illumination cultivation nitrogen source is ammonia nitrogen, and its initial concentration is between 40-160mg.L-1, preferably 80mg.L-1, it is passed through air or air and CO2Mixed gas, ventilation 50-300L/h, preferably 80-120L/h；CO2Concentration 0.1-15%, uses 10-200 μm ol.m in incubation-2s-1Sun exposure, pH value controls, between 5-9, to be preferred with 7.0；Depending on total incubation time visual cell growing state, it is typically in the range of 4-400 hour, preferably 12-48 hour；
(5) limit the microalgae cell efficient absorption height ammonia nitrogen nutrient of nitrogen cultivation and purify waste water:
The limit nitrogen cell of results proceeds to high ammonia-nitrogen wastewater cultivate, absorb ammonia nitrogen in high density and purify waste water.
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CN116375210A (en) * 2023-03-13 2023-07-04 江门职业技术学院 Microbial fuel cell constructed wetland system and method for strengthening freshwater fishery culture tail water treatment
CN116375210B (en) * 2023-03-13 2023-09-08 江门职业技术学院 Microbial fuel cell constructed wetland system and method for strengthening freshwater fishery culture tail water treatment

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