CN109251866A - One chlamydomonas strain and its application in biogas slurry purification - Google Patents
One chlamydomonas strain and its application in biogas slurry purification Download PDFInfo
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Abstract
The present invention proposes a chlamydomonas strain, deposit number are as follows: CGMCC NO.15497.The present invention also proposes application of the chlamydomonas strain in biogas slurry purification.Deposit number proposed by the present invention is the chlamydomonas of CGMCC NO.15497, under suitable condition of culture, it can be grown in pig manure biogas slurry, using the pollutant ammonia nitrogen in pig manure biogas slurry as own growth nitrogen source, using the pollutant total phosphorus in pig manure biogas slurry as own growth phosphorus source, the catharsis of denitrogenation dephosphorizing is played to pig manure biogas slurry.Compared with the chlamydomonas strain of other business algae libraries purchase, deposit number of the invention is that the chlamydomonas of CGMCC NO.15497 all has a clear superiority either in the accumulation of biomass or in the effect to pig manure biogas slurry denitrogenation dephosphorizing.
Description
Technical field
The invention belongs to microorganism fields, and in particular to a kind of chlamydomonas and its application in biogas slurry purification.
Background technique
It takes methane engineering technology to handle poultry waste, while harvesting the energy, realizes pollution control, can obtain
The multiple benefits such as environmental protection and economy, are the effective means for solving the problems, such as Animal manure loss.But the hair of large and medium-sized biogas project
Exhibition itself is also faced with many environmental problems, and how the biogas slurry generated after livestock and poultry feces anaerobic fermentation among these handles asking of utilizing
It inscribes especially pronounced.The biogas slurry amount that large and medium-sized biogas project generates daily is very considerable, only the biogas slurry Jing Guo Anaerobic Treatment, wherein still
Containing nutritional ingredients such as a large amount of nitrogen, phosphorus, it is directly discharged into water body and easily causes eutrophication, cause secondary pollution.Meanwhile it is most of
Large and medium-sized biogas project majority build suburbs in, and the on-site elimination of biogas slurry has certain difficulty, and disposable consumption is far more than farming
The universal law of object fertilising, if long-distance sand transport, and in the presence of energy consumption is high, problem at high cost.Therefore, how on the spot to biogas slurry into
Row low cost, depth processing is the large and medium-sized biogas project of development one of main problem urgently to be resolved.
Biogas slurry is residual liquid after anaerobic fermentation, the main organic and inorganic salt including decomposing release in fermentation process,
Such as ammonium salt, sylvite, phosphate solable matter, total solids content is less than 1%.Compared with biogas residue, the nutrient in biogas slurry is main
It is quick-acting nutrient, shows according to the study, in livestock and poultry feces biogas slurry not only in nitrogen rich in, phosphorus, potassium, calcium, magnesium, iron, manganese etc.
Microelement, also containing plant growth regulating substances and quinolinone, carbohydrate, dimensions such as heteroauxin, the basic element of cell division, gibberellin
The bioactive ingredients such as raw element, polyamines, therefore, although biogas slurry is a kind of waste water in itself, if be rationally used, it
It is a kind of resource of nutritional ingredient very abundant.
Microalgae be it is a kind of can fast-growth breeding photoautotrophy aquatic microorganisms, its photosynthetic efficiency is high, eucaryotic cell structure letter
Single, the ability for adapting to environment is strong, high to nutrient utilization rate in environment, and cultivates microalgae and do not need the area that occupies cultivated land.It is micro-
Algal biomass both can be used as the refinement original of bio-fuel-oil, biological natural pigment (carotenoid, astaxanthin, phycocyanin etc.)
Material, and the health food and animal feed, aquatic feed of the mankind can be processed into, additionally it is possible to soil is imposed on as organic slow-release fertilizer
Middle promotion plant growth, thus be that a kind of added value is high, widely used microorganism raw material.Artificial culture microalgae, which needs to contain, fills
The fluid nutrient medium of the nutrients such as carbon, nitrogen, the phosphorus of foot, and the nutritional ingredient in some organic wastewaters and microdisk electrode base class
Seemingly, thus using waste water culture microalgae the hot spot in Microalgae biotechnology research has been rapidly become.Microdisk electrode is big in addition to needing
Other than the nutritive salt of amount, it is also necessary to consume a large amount of water resource, the cost of micro-algae culture medium accounts for microdisk electrode totle drilling cost
30%-60%.Using biogas slurry culture microalgae, microalgae can be absorbed the nutrients such as carbon, nitrogen, phosphorus in biogas slurry and promote itself to give birth to
It is long, play the role of purification to biogas slurry in the process.
Livestock/poultry biogas slurry is purified using microalgae, the important technological difficulties of one of them are, finding has for specific biogas slurry
The microalgae algae strain of good tolerability, algae strain can grow in the specific biogas slurry within the scope of a certain concentration, absorb in biogas slurry
Nitrogen and phosphorus pollutants are and toxic to ammonia nitrogen, pathogenic microorganism, breeding feed medicament residue in biogas slurry etc. as itself nutrient source
Harmful substance has tolerance.
Summary of the invention
Aiming at the problem that mentioning in above-mentioned background, the present invention separates from the soil environment polluted by pig manure biogas slurry, is pure
Change and obtain one plant of wild microalgae, this plant of microalgae can be grown in pig manure biogas slurry, and absorb the dirts such as the nitrogen phosphorus in pig manure biogas slurry
Dye object promotes algae somatic growth as the nitrogen and phosphorus source of itself, while the work well purified can be played to pig manure biogas slurry
With.Meanwhile there is certain tolerance to the poisonous and harmful substance in pig manure biogas slurry；That is, the first purpose of this invention is to mention
A kind of chlamydomonas strain out.
Second object of the present invention is to propose the application of the chlamydomonas strain.
Realize the technical solution of the object of the invention are as follows:
One plant of chlamydomonas (Chlamydomonas sp.) algae strain, deposit number are as follows: CGMCC NO.15497.
Above-mentioned chlamydomonas strain is preserved in Institute of Microorganism, Academia Sinica China Microbiological bacterium on March 29th, 2018
Kind preservation administration committee common micro-organisms center (CGMCC) (Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3).
Application of the chlamydomonas strain in biogas slurry purification.
A kind of method of biogas slurry purification, using the chlamydomonas strain, comprising:
The culture solution of the chlamydomonas strain is inoculated in biogas slurry with the inoculum concentration of 0.01~0.1g/L of algae dry weight, it is described
The COD of biogas slurry be 200~800mg/L, ammonia nitrogen be 40~150mg/L, total phosphorus be 10~50mg/L, suspended solid be 0~
500mg/L, pH value are 7.5~8.5.
In inoculum concentration, g refers to the dry weight of microalgae, can be measured with specific gravity in oven dry method；L refers to the volume of culture of microalgae, liquid
Ingredient is mainly biogas slurry, and microalgae cell may be regarded as solid particle.
Further, the culture solution of the chlamydomonas strain is inoculated in the inoculum concentration of 0.02~0.05g/L of algae dry weight
In biogas slurry.
Pure culture is carried out to microalgae if it is in artificial medium, then in order to obtain more biomass as far as possible, generally
Inoculum concentration is in 0.02~0.03g/L or so.But it is inoculated into inside biogas slurry, it is contemplated that various complex materials are to microalgae in biogas slurry
Impact, need to properly increase inoculum concentration, and not so inoculum concentration is likely to that microalgae is resistance to can't stand biogas slurry just in the dust as pure culture.
The optimal value that we determine after many experiments is in 0.03~0.05g/L or so.
Wherein, the culture solution of the chlamydomonas strain is cultivated the chlamydomonas strain in liquid medium to logarithm life
Obtained by long-term；Inoculum concentration is 0.02~0.025g/L fluid nutrient medium；And/or the fluid nutrient medium is BG11.
Wherein, the condition that the chlamydomonas strain is cultivated in liquid medium are as follows: shining intensity is 100~300 μm of ol/m2/
S, photoperiod are Light To Dark Ratio (12~18): (6~12), and temperature is 15~30 DEG C.
Preferably, the condition that the chlamydomonas strain is cultivated in liquid medium are as follows: intensity of illumination is 170~190 μm of ol/
㎡/s, photoperiod are Light To Dark Ratio 18:6, and temperature is 26 ± 0.5 DEG C.
Wherein, it when the chlamydomonas strain is cultivated in liquid medium, is shaken every day culture vessel 2~4 times.
A kind of optimal technical scheme of the present invention is, the method comprising steps of
1) pig manure biogas slurry reaches 200~800mg/L of COD by pretreatment, 40~150mg/L of ammonia nitrogen, and total phosphorus 10~
50mg/L, 0~500mg/L of suspended solid, the condition that pH value is 7.5~8.5；
2) culture solution that the chlamydomonas strain is added is grown, the condition of growth are as follows: intensity of illumination is 100~200 μ
Mol/ ㎡/s, photoperiod are Light To Dark Ratio (12~20): (4~12), and temperature is 24~28 DEG C.
Wherein, it is described pretreatment for flocculation and air-flotation process, pig manure biogas slurry by pretreatment, reach COD 300~
450mg/L, 840~120mg/L of ammonia nitrogen, 20~30mg/L of total phosphorus, 0~300mg/L of suspended solid, the item that pH value is 8.0~8.5
Part.
The existing processing mode in this field can be used in flocculation and air-flotation process.
The beneficial effects of the present invention are:
Deposit number proposed by the present invention is that the chlamydomonas of CGMCC NO.15497 can be in pig under suitable condition of culture
It is grown in excrement biogas slurry, using the pollutant ammonia nitrogen in pig manure biogas slurry as own growth nitrogen source, utilizes the pollution in pig manure biogas slurry
Object total phosphorus plays the catharsis of denitrogenation dephosphorizing to pig manure biogas slurry as own growth phosphorus source.It is bought with other business algae libraries
Chlamydomonas strain compare, deposit number of the invention be CGMCC NO.15497 chlamydomonas either in the accumulation of biomass also
It is all to have a clear superiority in the effect to pig manure biogas slurry denitrogenation dephosphorizing.
Detailed description of the invention
Fig. 1 be chlamydomonas strain CGMCC NO.15497 of the present invention under an optical microscope cellular morphology (10 times of eyepiece ×
40 times of object lens).
Fig. 2 is the phylogenetic tree based on chlamydomonas strain CGMCC NO.15497 building in the present invention.
Fig. 3 is that the present invention isolates and purifies the chlamydomonas strain CGMCC NO.15497 of acquisition with other two plants from business algae library
Growth pair of Chlamydomonas reinhardtii algae the strain FACHB-265 and chlamydomonas strain FACHB-1935 of stochastic buying in pig manure biogas slurry in 20 days
Than figure.
Fig. 4 is that the present invention isolates and purifies the chlamydomonas strain CGMCC NO.15497 of acquisition with other two plants from business algae library
Chlamydomonas reinhardtii algae strain two plants of microalgaes of FACHB-265 and chlamydomonas strain FACHB-1935 of stochastic buying are in pig manure biogas slurry in 20 days
Comparison to ammonia nitrogen removal.
Fig. 5 is that the present invention isolates and purifies the chlamydomonas strain CGMCC NO.15497 of acquisition with other two plants from business algae library
Chlamydomonas reinhardtii algae strain two plants of microalgaes of FACHB-265 and chlamydomonas strain FACHB-1935 of stochastic buying are in pig manure biogas slurry in 20 days
Comparison to total phosphorus ligands.
Specific embodiment
With reference to the accompanying drawings and examples, a specific embodiment of the invention is described in further detail.
Those skilled in the art should know following embodiment is merely to illustrate the present invention, but is not limited to the present invention
Range.
Embodiment 1: separation, purifying and the preservation of algae strain
(1) sample acquires
Algae strain according to the present invention is out of Pinggu district Daxing the village village Zhen Xi Bai Dian biogas service station through pig manure
It is sampled in the soil that anaerobic digestion biogas slurry polluted isolated.Soil sample is acquired from siting upper soll layer, is placed in sealing
In bag, it is placed in 4 DEG C of refrigerators and saves backup.
(2) algae strain separation, purifying and algae strain culture
It takes about 0.5g pedotheque in 24 hole tissue culture dishes, and BG11 fluid nutrient medium (BG11 culture medium tool is added
Body composition is as shown in table 1) it is placed in progress algae enrichment culture in the adjustable illumination box of environmental parameter, after culture 3-4 days,
Culture medium color, which can clearly be seen that from colourless, becomes light green.Then BG11 solid plate culture medium (sterilized processing
BG11 fluid nutrient medium be added 1.5%-2% agar powder be prepared) on isolated and purified using plate streak, until
Algae falls single in plate, and then the sterile single algae of picking, which falls, is inoculated into liquid B G11 culture medium, sets and trains in the light incubator
It supports.With the microalgae algae strain of optical microscopy observation after cultivation, whether cellular morphology is consistent, achievees the purpose that separation if consistent,
The work of plate streaking is repeated if inconsistent, until cellular morphology is single.
Culture vessel used in algae strain cultivation stage is 100mL conical flask (effective volume of culture is 50mL), BG11 liquid
Body culture medium, condition of culture are 28 ± 0.5 DEG C of temperature, 150 μm of ol/m of intensity of illumination2/ s, periodicity of illumination 12:12, it is artificial daily
Shaking flask is three times.
1 BG11 culture medium prescription of table and dosage
The identification of algae strain
The identification of algae strain carries out in two steps, first progress morphology preliminary observation, then carries out molecular biology again
Identification.The microalgae algae strain isolated and purified is observed using optical microscopy and takes a picture (10 times of eyepiece × 40 times object lens).It sees
Examine the features such as cellular morphology, size, the structure of algae strain.Shown in algae plant shape state attached drawing 1 under optical microphotograph sem observation.
The present invention isolates and purifies this plant of microalgae of acquisition, observes its biological characteristics under the microscope and is, frond is in green,
Unicellular, cell is spherical in shape or oval, and there are two isometric flagellums in front end, can move about.
Molecular biology identification is carried out to the microalgae, it is soft using DNAMAN and Primer 5.0 using the DNA of extraction as template
Part design primer, (primer sequence forward direction 5'-TCCGTAGGTGAACCTGCGG-3', reversed 5'-TCCTCCGCTTATTGATATGC-
3').PCR amplification is carried out to ITS gene, establishing PCR reaction system is 50 μ L.Wherein 1 μ L, positive each 1 μ of anti-primer of DNA profiling
L, dNTP4 μ L, 5 × Q5 react 10 μ L, Q5DNA polymerase of buffer solution, 0.5 μ L, 5 × Q5High GC Enhancer, 10 μ L.
ITS gene PCR amplification program are as follows: 94 DEG C of initial denaturation 30s, then 98 DEG C of denaturation 5s, 52 DEG C of annealing 30s, 68 DEG C of extension 75s, total
30 circulations, last 68 DEG C of extensions 5min.The process entrusts Beijing Ai Puxilong Biotechnology Co., Ltd to be sequenced.By gained sequence
It is listed in GenBank database (http://www.ncbi.nlm.nih.gov/) and carries out homologous detection with BLAST, in algae library
Existing algae gene is compared, and finally determines its kind.After carrying out PCR amplification to algae strain, the segment of ITS is obtained.After sequencing
The length for obtaining its ITS segment is 694bp.This sequence and ncbi database are subjected to homology analysis, find itself and chlamydomonas
The affiliation of (Chlamydomonas sp.) is nearest, and homology reaches 99%.ITS sequence is constructed using MEGA5.10 and is
System development tree, as a result as shown in Figure 2.
The preservation of algae strain
The above-mentioned chlamydomonas strain for being isolated and purified acquisition and being identified, it is micro- to be preserved in the Chinese Academy of Sciences on March 29th, 2018
China Committee for Culture Collection of Microorganisms's common micro-organisms center of biological study institute (CGMCC) (Chaoyang District, Beijing City North Star
The institute 3 of West Road 1), deposit number is CGMCC NO.15497.
Embodiment 2 algae strain optimal culture conditions are probed into
After the above-mentioned microalgae algae for obtaining enough biomass, to probe into its suitable condition of culture, the present invention is to illumination
The common technical parameter of four intensity, photoperiod, temperature, inoculum concentration microdisk electrodes has carried out single factor experiment-orthogonal test-
150,200,250,300 the series of optimum research such as response surface experiments, the parameter of setting are intensities of illumination: 100, (μm ol/ ㎡/
S), photoperiod (light dark ratio): 12/12,14/10,16/8,18/6；Temperature: 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C；Pure culture inoculation
Measure (culture of algae culture medium): 0.015g/L, 0.020g/L, 0.025g/L, 0.030g/L have found that optimal is 0.025g/L.Together
Test method, the setting biogas slurry culture inoculum concentration (culture that algae is used to purify pig manure biogas slurry) of sample: 0.020g/L, 0.030g/
L, 0.040g/L, 0.050g/L, test find that optimal inoculum concentration is 0.041g/L.
It is 180 μm of ol/ ㎡/s, photoperiod 18:6 that the optimal culture conditions for finally obtaining this plant of microalgae, which are intensity of illumination,
(light: dark), temperature is 26 ± 0.5 DEG C, inoculum concentration 0.022g/L.The culture vessel that the stage uses is that 250mL conical flask (has
Effect volume of culture is 150mL), BG11 fluid nutrient medium is placed in the adjustable illumination box of environmental parameter, daily artificial shaking flask
Three times.
To probe into the chlamydomonas strain CGMCC NO.15497 of above-mentioned separation, purifying, preservation in terms of purifying pig manure biogas slurry
Potentiality, the present invention have carried out the experimental study using true pig manure biogas slurry culture chlamydomonas strain CGMCC NO.15497.In the examination
Test in research, other than chlamydomonas CGMCC NO.15497 algae of the present invention strain, be investigated other two plants it is (comparative example 1, right
Ratio 2) ability for the chlamydomonas strain purification pig manure biogas slurry bought is randomly choosed from business algae library and its in pig manure biogas slurry
Upgrowth situation, to be compared with algae strain of the present invention.
In embodiment 3 and comparative example, the culture solution measurement biomass and water quality of 6mL are sampled daily.All test groups are all provided with
Set three repetitions.
Embodiment 3: pig manure biogas slurry is purified using chlamydomonas strain CGMCC NO.15497
(1) test material
Pig manure biogas slurry used in this test is derived from the village the village Zhen Xi Bai Dian biogas service station biogas slurry storage of Pinggu district Daxing
In tank, because of the ammonia nitrogen (> 900mg/L) and a large amount of suspended solid (700- in the former biogas slurry in the holding vessel containing higher concentration
800 mg/L), flocculate to former biogas slurry-pneumatically supported pretreatment, so that ammonia nitrogen concentration and suspended sediment concentration drop to microalgae
The degree being resistant to.Water quality by pretreated pig manure biogas slurry is as shown in table 2.
The pig manure biogas slurry water quality after pretreatment of table 2
The algae strain of this test is the chlamydomonas strain CGMCCNO.15497 of above-mentioned present invention separation, purifying, using implementation
The optimal culture conditions that example 2 optimizes are used for this experimental study in BG11 culture medium after culture to logarithmic growth phase.
(2) test method
Microalgae in logarithmic growth phase is added in 250mL conical flask (effective volume of culture 150mL) and adds pig manure
Biogas slurry is placed in the adjustable illumination box of environmental parameter and cultivates as micro-algae culture medium, specific condition of culture are as follows: inoculum concentration
150 μm of ol/m of 0.041g/L intensity of illumination2/ s, photoperiod 18:6 (light: dark), 26 ± 0.5 DEG C of temperature.All test groups co-culture
20 days, daily artificial shaking flask was three times.
Comparative example 1
The algae strain of this comparative example is the Rhein bought from the random selection of the aquatic institute's fresh water algae library in Chinese Academy of Sciences Wuhan
Chlamydomonas strain FACHB-265 is cultivated in BG11 culture medium to logarithmic growth phase using the optimal culture conditions of the optimization of embodiment 2
It is used for this experimental study afterwards.
Comparative example 2
The algae strain of this comparative example is the chlamydomonas bought from the random selection of the aquatic institute's fresh water algae library in Chinese Academy of Sciences Wuhan
Algae strain FACHB-1935.It is cultivated in BG11 culture medium to logarithmic growth phase using the optimal culture conditions optimized with embodiment 2
It is used for this experimental study afterwards.
Test result
Upgrowth situation of three plants of microalgaes in pig manure biogas slurry is as shown in Figure 3.In 20 days incubation times, Chlamydomonas reinhardtii algae strain
FACHB-265 can be grown with the chlamydomonas strain CGMCC NO.15497 of the invention for separating, purifying in pig manure biogas slurry, still
Growth ability difference is obvious.Wherein, growth result of the chlamydomonas strain CGMCC NO.15497 in pig manure biogas slurry is more preferable, and the 20th day
Biomass can reach 0.583g/L, and Chlamydomonas reinhardtii algae strain FACHB-265 was only capable of reaching 0.450g/L in the 20th day biomass.Clothing
Algae algae strain FACHB-1935 is then significantly inhibited in pig manure biogas slurry, and Initial stage of culture still can slowly be grown, from the 9th of test the
It starts, and cell starts to taper off, and biomass also declines therewith.Test result, which shows to randomly choose with business algae library, to be purchased
The chlamydomonas strain bought is compared, and it is best that the present invention isolates and purifies tolerance of the chlamydomonas strain of acquisition in pig manure biogas slurry, accumulation life
The ability of substance is also most strong.
The variation of ammonia nitrogen is as shown in Figure 4 in pig manure biogas slurry.In 20 days incubation times, three plants of microalgaes are in pig manure biogas slurry
The removal ability difference of ammonia nitrogen is obvious.Wherein, the chlamydomonas strain CGMCC NO.15497 of present invention separation, purifying is to pig manure biogas slurry
The removal ability of middle ammonia nitrogen is most strong, and the removal rate of 20 days ammonia nitrogens is up to 97.79%.FACHB-265 was at 20 days for Chlamydomonas reinhardtii algae strain
The interior ammonia nitrogen removal frank to pig manure biogas slurry is 81.33%.And due to chlamydomonas strain FACHB-1935 since the 9th day of culture by
Degradation is died, thus it is finally only 20.11% to the removal of ammonia nitrogen in pig manure biogas slurry.
The variation of total phosphorus is as shown in Figure 5 in pig manure biogas slurry.In 20 days incubation times, the present invention separation, purifying chlamydomonas
Algae strain CGMCC NO.15497 is 90.36% to the removal rate of total phosphorus in pig manure biogas slurry, and FACHB-265 is 20 for Chlamydomonas reinhardtii algae strain
It is 79.53% to the removal rate of total phosphorus in it, and since chlamydomonas strain FACHB-1935 gradually declined since the 9th day of culture
It dies, thus it is finally only 18.90% to the removal of ammonia nitrogen in pig manure biogas slurry.
In conclusion the chlamydomonas strain CGMCC NO.15497 that present invention separation, purifying obtain is to certain density pig manure
Biogas slurry has good tolerance, using pig manure biogas slurry this plant of microalgae of culture, good purification can be played to pig manure biogas slurry and is made
With the significant effect of denitrogenation dephosphorizing.It is of the present invention this from certainly compared with two plants of chlamydomonas of business algae library stochastic buying
The wild algae strain separated in right environment, the growth ability in pig manure biogas slurry is more preferable, thus is a kind of reason for handling pig manure biogas slurry
Think microbial material, has broad application prospects.
Above embodiment be only preferred embodiments of the present invention will be described, not to the scope of the present invention into
Row limits, and without departing from the spirit of the design of the present invention, this field ordinary engineering and technical personnel is to technical side of the invention
The all variations and modifications that case is made, should fall within the scope of protection determined by the claims of the present invention.
Sequence table
<110>China Agricultural University
<120>one chlamydomonas strains and its application in biogas slurry purification
<130> KHP181115747.5
<141> 2018-10-10
<160> 2
<170> SIPOSequenceListing 1.0
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<211> 19
<212> DNA
<213> Artificial sequence
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tccgtaggtg aacctgcgg 19
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<212> DNA
<213> Artificial sequence
<400> 2
tcctccgctt attgatatgc 20
Claims (10)
1. a chlamydomonas strain, which is characterized in that deposit number are as follows: CGMCC NO.15497.
2. application of the chlamydomonas strain described in claim 1 in biogas slurry purification.
3. a kind of method of biogas slurry purification, using chlamydomonas strain described in claim 1 characterized by comprising
The culture solution of the chlamydomonas strain is inoculated in biogas slurry with the inoculum concentration of 0.01~0.1g/L of algae dry weight, the biogas slurry
COD be 200~800mg/L, ammonia nitrogen be 40~150mg/L, total phosphorus be 10~50mg/L, suspended solid be 0~500mg/L,
PH value is 7.5~8.5.
4. the method for biogas slurry purification according to claim 3, which is characterized in that with connecing for 0.02~0.05g/L of algae dry weight
The culture solution of the chlamydomonas strain is inoculated in biogas slurry by kind amount.
5. the method for biogas slurry purification according to claim 3, which is characterized in that the culture solution of the chlamydomonas strain is by institute
Chlamydomonas strain is stated to be cultivated in liquid medium to obtained by logarithmic growth phase；Inoculum concentration is 0.02~0.025g/L Liquid Culture
Base；And/or the fluid nutrient medium is BG11.
6. the method for biogas slurry purification according to claim 5, which is characterized in that the chlamydomonas strain is trained in liquid medium
Feeding condition are as follows: shining intensity is 100~300 μm of ol/m2/ s, photoperiod are Light To Dark Ratio (12~18): (6~12), temperature 15
~30 DEG C.
7. the method for biogas slurry purification according to claim 6, which is characterized in that the chlamydomonas strain is trained in liquid medium
Feeding condition are as follows: intensity of illumination is 170~190 μm of ol/ ㎡/s, and the photoperiod is Light To Dark Ratio 18:6, and temperature is 26 ± 0.5 DEG C.
8. according to the method for any one of the claim 4~7 biogas slurry purification, which is characterized in that the chlamydomonas strain is in liquid
When being cultivated in culture medium, it is shaken every day culture vessel 2~4 times.
9. according to the method for any one of the claim 3~7 biogas slurry purification, which is characterized in that comprising steps of
1) pig manure biogas slurry reaches 200~800mg/L of COD, 40~150mg/L of ammonia nitrogen, 10~50mg/L of total phosphorus by pretreatment,
0~500mg/L of suspended solid, the condition that pH value is 7.5~8.5；
2) culture solution that the chlamydomonas strain is added is grown, the condition of growth are as follows: intensity of illumination is 100~200 μm of ol/m2/
S, photoperiod are Light To Dark Ratio (12~20): (4~12), and temperature is 24~28 DEG C.
10. according to claim 9 biogas slurry purification method, which is characterized in that it is described pretreatment for flocculation and air-flotation process,
Pig manure biogas slurry reaches 300~450mg/L of COD, 840~120mg/L of ammonia nitrogen, 20~30mg/L of total phosphorus by pretreatment, suspends
0~300mg/L of solid, the condition that pH value is 8.0~8.5.
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| CN114606131A (en) * | 2022-03-30 | 2022-06-10 | 福建师范大学 | Green alga strain and application thereof in rare earth ammonia nitrogen wastewater treatment |
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