CN105853743A - Traditional Chinese medicine preparation for removing chloasma as well as preparation method and application thereof - Google Patents
Traditional Chinese medicine preparation for removing chloasma as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN105853743A CN105853743A CN201610377077.XA CN201610377077A CN105853743A CN 105853743 A CN105853743 A CN 105853743A CN 201610377077 A CN201610377077 A CN 201610377077A CN 105853743 A CN105853743 A CN 105853743A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- parts
- medicine preparation
- preparation
- concentrated solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/716—Clematis (leather flower)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a traditional Chinese medicine preparation for removing chloasma as well as a preparation method and application thereof. The traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 3-10 parts of sesame oil, 18-26 parts of ardisia pusilla, 8-16 parts of clematis root, 10-18 parts of peach kernels, 10-18 parts of Chinese pholidota herb and 1-8 parts of brucea fruits. The preparation method of the traditional Chinese medicine preparation comprises the following steps: smashing and sieving the ardisia pusilla, Chinese pholidota herb and brucea fruits, adding anhydrous alcohol, carrying out sealed heat insulation processing, ultrasonic processing and centrifugal separation, taking supernatant liquid, and concentrating the supernatant liquid to obtain a concentrated solution A; grinding, smashing and sieving the peach kernels, carrying out extraction with hot water for three times, mixing extraction solutions, and concentrating the mixed solution to obtain a concentrated solution B; grinding, smashing and sieving the clematis root, adding vinegar for baking, adding water for decocting, filtering the decoction, and then taking filtrate; and carrying out mixed heat insulation processing on the concentrated solution A, the concentrated solution B and the filtrate, adding sesame oil, and carrying out ultrasonic processing and concentration to obtain extract, namely the traditional Chinese medicine preparation. The traditional Chinese medicine preparation is capable of improving skin chloasma and removing moles, has obvious curative effects, and has the characteristics of being convenient to smear, low in medication cost, high in effect taking speed, free of toxic or side effect and capable of treating both symptoms and root causes.
Description
Technical field
The present invention relates to technical field of pharmaceuticals, Chinese medicine preparation of a kind of speckle dispelling and its preparation method and application.
Background technology
Chloasma is the filbert or dark brown of a kind of obscure boundary Chu betided around buccal, forehead, eye, nose and lip
Patch shape dermatosis, is commonly called as " butterfly spot ", " chloasma hepaticum " or " cyasma ", mostly is symmetry, relevant with endocrine,
Especially relevant with the estrogen level of women, menoxenia, gestation, take contraceptive or liver function is bad and chronic nephropathy
All it is likely to occur chloasma.In addition Exposure to Sunlight and Nervous and Mental Factors also can increase the weight of primary disease.Anemia of pregnant woman usually occurs in gestation for 3 months later
Chloasma, majority are gradually disappeared when after childbirth, menstruation recovery is normal.If do not moved back for a long time, need to treat.
Doctor trained in Western medicine is for its main pathology in treatment, and preventing and treating is smeared based on medicine for external use, suitably coordinates the side such as oral medicine, massage
Method, the most conventional medicine for external use has 3% Hydrochinone Cream etc., and effect is unsatisfactory.Some whitening cosmetics are had to contain on the market
There are the whitening compositions such as arbutin, vitamin C/E and derivant thereof, Flavonoids and Polyphenols extract, nicotiamide, really
The treatment such as skin and vitamin C iontophoresis is changed in acid, and oral JIAWEI XIAOYAO SAN, TAOHONG SIWU TANG etc., external used medicine can not be dispelled
The cause of disease, easily recurs, it is impossible to take effect in a short time.The treatment of chloasma is relatively difficult in a word, takes effect slow, and a lot of medicines face
Bed weak curative effect, and treatment time is long, and side effect is big, and modal is exactly injury of gastrointestinal tract, the feature such as the most repeatedly.
Black naevus is medically referred to as " nevus cell nevus ", is the benign tumor of skin, by neural cellular differentiation out, different
Age, dissimilarity others all may long black naevus.Doctor trained in Western medicine mainly use freezing, fulgerize, the physical therapy treatment such as laser.In
Doctor then uses oral Chinese medicine, treats discharging internal virus.Physical therapy treatment can not reach the curative effect of 100%, and
The Chinese traditional treatment oral Chinese medicine length course for the treatment of, effect are undesirable.
Summary of the invention
It is an object of the invention to provide that a kind of coating is convenient, drug cost is low, medication instant effect, have no side effect, specimen
Chinese medicine preparation of speckle dispelling taken into account and its preparation method and application, with the problem solving to propose in above-mentioned background technology.
For achieving the above object, the present invention provides following technical scheme:
The Chinese medicine preparation of a kind of speckle dispelling, is made up of according to the raw material of weight portion following: Oleum sesami 3-10 part, A.pusilla A.DC. 18-26
Part, Radix Clematidis 8-16 part, Semen Persicae 10-18 part, Pseudobulbus Pholidotae Chinensis 10-18 part, Fructus Bruceae 1-8 part.
As the further scheme of the present invention: the Chinese medicine preparation of described speckle dispelling, it is made up of according to the raw material of weight portion following: sesame
Oleum Sesami 5-8 part, A.pusilla A.DC. 20-24 part, Radix Clematidis 10-14 part, Semen Persicae 12-16 part, Pseudobulbus Pholidotae Chinensis 12-16 part, Fructus Bruceae
3-6 part.
As the further scheme of the present invention: the Chinese medicine preparation of described speckle dispelling, it is made up of according to the raw material of weight portion following: sesame
7 parts of Oleum Sesami, A.pusilla A.DC. 22 parts, Radix Clematidis 12 parts, 14 parts of Semen Persicae, Pseudobulbus Pholidotae Chinensis 14 parts, Fructus Bruceae 5 parts.
The preparation method of the Chinese medicine preparation of described speckle dispelling, comprises the steps of:
1) A.pusilla A.DC., Pseudobulbus Pholidotae Chinensis, Fructus Bruceae are pulverized, are crossed 100 mesh sieves, add the dehydrated alcohol of three's mass 12-14 times,
At 60-65 DEG C of lower seal isothermal holding 1.5-2h, at 60-65 DEG C, then carry out supersound process 1-1.2h, sonification power
For 900W, carry out centrifugation after supersound process, take supernatant, be then concentrated into the 20-25% of original volume, prepare and concentrate
Liquid A;
2) Semen Persicae ground, cross 200 mesh sieves, then by the hot water extraction 3 times of 70-75 DEG C, each hot water addition
For 6-8 times of Semen Persicae quality, extraction time is 2-2.5h, merges lixiviating solution, is concentrated into the 20-25% of original volume, prepares dense
Contracting liquid B;
3) Radix Clematidis ground, cross 100 mesh sieves, be subsequently adding the vinegar of its quality 1-1.2 times, at 70-75 DEG C
Baking 1-1.2h, is subsequently adding the water of its quality 8-10 times, and intense fire is boiled, slow fire boiling 1.2-1.5h;Filtrate is taken after filtration;
4) by concentrated solution A, concentrated solution B, filtrate mixing, it is warming up to 85-90 DEG C, and isothermal holding 0.5-0.8h at such a temperature,
Being subsequently adding Oleum sesami, and carry out supersound process 0.5-0.8h at 60-65 DEG C, sonification power is 900W, is condensed into
Relative density be 1.15 extractum i.e. obtain Chinese medicine preparation.
The application in treatment medicine for removing speckle of the described Chinese medicine preparation.
Lecithin in Oleum sesami not only skin care in the present invention, and can be with speckle dispelling, it is particularly possible to dispel senile plaque.
A.pusilla A.DC. expelling wind and removing dampness, promoting blood circulation and stopping pain.Dispelling the wind and dampness pathogens pain, treating swelling and pain by traumatic injury, cough haematemesis, abdominal pain due to cold.
Radix Clematidis expelling wind and removing dampness, removing obstruction in the collateral to relieve pain.For rheumatic arthralgia, numb limbs and tense tendons, muscle arteries and veins contracture, joint stuffiness, bone is choked
Throat.
Semen Persicae has blood circulation promoting and blood stasis dispelling, loosening bowel to relieve constipation, effect of relieving cough and asthma.For amenorrhea dysmenorrhea, abdominal mass abdominal mass mass in the abdomen, lung abscess intestinal
Carbuncle, injury from falling down, dryness of the intestine constipation, cough and asthma.
Fructus Bruceae heat-clearing and toxic substances removing, preventing the attack (or recurrence) of malaria, dysentery relieving;External corrosion wart.Cure mainly dysentery, malaria;External treatment wart, clavus.
Pseudobulbus Pholidotae Chinensis has nourishing YIN and moistening the lung, heat-clearing and toxic substances removing, dampness removing, effect of repercussive.It is usually used in cough due to lung-heat, hemoptysis, spits blood,
Dizziness, headache, nocturnal emission, laryngopharynx swelling and pain, rheumatalgia, damp and hot edema, dysentery, leucorrhea, infantile malnutrition, scrofula, traumatic injury damages
Wound.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention is proved by a large amount of zooperies, has the pharmacodynamic action of whitening and speckle dispelling, has melanoma cells and substantially presses down
Make and use.The present invention is formed by multiple prepared from traditional Chinese medicines, can dredging the meridian, benefiting qi and nourishing blood under the synergism of each raw material
Change addiction, skin chloasma can be improved, dispel black naevus, evident in efficacy.Medicine of the present invention also has coating convenience, drug cost
Low, medication instant effect, have no side effect, the feature of giving consideration to both the incidental and fundamental.The present invention can suppress the development of the state of an illness, makes skin reply
Level in the past, and can substantially shorten the course of disease, therapeutic effect is obvious, is worthy of popularization.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, aobvious
So, described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, all
Belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, the Chinese medicine preparation of a kind of speckle dispelling, it is made up of according to the raw material of weight portion following: Oleum sesami 3 parts,
A.pusilla A.DC. 18 parts, Radix Clematidis 8 parts, 10 parts of Semen Persicae, Pseudobulbus Pholidotae Chinensis 10 parts, Fructus Bruceae 1 part.
A.pusilla A.DC., Pseudobulbus Pholidotae Chinensis, Fructus Bruceae are pulverized, crossed 100 mesh sieves, adds the dehydrated alcohol of three's mass 12 times,
60 DEG C of lower seal isothermal holding 1.5h, then carry out supersound process 1h at 60 DEG C, and sonification power is 900W, ultrasonic
Carry out centrifugation after process, take supernatant, be then concentrated into the 20% of original volume, prepare concentrated solution A.Semen Persicae is ground
Pulverizing, cross 200 mesh sieves, then with the hot water extraction 3 times of 70 DEG C, each hot water addition is 6 times of Semen Persicae quality, soaks
The time of carrying is 2h, merges lixiviating solution, is concentrated into the 20% of original volume, prepares concentrated solution B.Radix Clematidis is ground, mistake
100 mesh sieves, are subsequently adding the vinegar of its quality 1 times, toast 1h, be subsequently adding the water of its quality 8 times at 70 DEG C, military
Fire is boiled, slow fire boiling 1.2h;Filtrate is taken after filtration.By concentrated solution A, concentrated solution B, filtrate mixing, it is warming up to 85 DEG C,
And isothermal holding 0.5h at such a temperature, it is subsequently adding Oleum sesami, and at 60 DEG C, carries out supersound process 0.5h, supersound process
Power is 900W, is condensed into the extractum that relative density is 1.15 and i.e. obtains Chinese medicine preparation.
Embodiment 2
In the embodiment of the present invention, the Chinese medicine preparation of a kind of speckle dispelling, it is made up of according to the raw material of weight portion following: Oleum sesami 10
Part, A.pusilla A.DC. 26 parts, Radix Clematidis 16 parts, 18 parts of Semen Persicae, Pseudobulbus Pholidotae Chinensis 18 parts, Fructus Bruceae 8 parts.
A.pusilla A.DC., Pseudobulbus Pholidotae Chinensis, Fructus Bruceae are pulverized, crossed 100 mesh sieves, adds the dehydrated alcohol of three's mass 14 times,
65 DEG C of lower seal isothermal holding 2h, then carry out supersound process 1.2h at 65 DEG C, and sonification power is 900W, ultrasonic
Carry out centrifugation after process, take supernatant, be then concentrated into the 25% of original volume, prepare concentrated solution A.Semen Persicae is ground
Pulverizing, cross 200 mesh sieves, then with the hot water extraction 3 times of 75 DEG C, each hot water addition is 8 times of Semen Persicae quality, soaks
The time of carrying is 2.5h, merges lixiviating solution, is concentrated into the 25% of original volume, prepares concentrated solution B.Radix Clematidis is ground,
Cross 100 mesh sieves, be subsequently adding the vinegar of its quality 1.2 times, at 75 DEG C, toast 1.2h, be subsequently adding its quality 10 times
Water, intense fire boils, slow fire boiling 1.5h;Filtrate is taken after filtration.By concentrated solution A, concentrated solution B, filtrate mixing, rise
Temperature is to 90 DEG C, and isothermal holding 0.8h at such a temperature, is subsequently adding Oleum sesami, and carries out supersound process 0.8h at 65 DEG C,
Sonification power is 900W, is condensed into the extractum that relative density is 1.15 and i.e. obtains Chinese medicine preparation.
Embodiment 3
In the embodiment of the present invention, the Chinese medicine preparation of a kind of speckle dispelling, it is made up of according to the raw material of weight portion following: Oleum sesami 5 parts,
A.pusilla A.DC. 20 parts, Radix Clematidis 10 parts, 12 parts of Semen Persicae, Pseudobulbus Pholidotae Chinensis 12 parts, Fructus Bruceae 3 parts.
A.pusilla A.DC., Pseudobulbus Pholidotae Chinensis, Fructus Bruceae are pulverized, crossed 100 mesh sieves, adds the dehydrated alcohol of three's mass 13 times,
62 DEG C of lower seal isothermal holding 1.8h, then carry out supersound process 1.1h at 62 DEG C, and sonification power is 900W, super
Carry out centrifugation after sonication, take supernatant, be then concentrated into the 22% of original volume, prepare concentrated solution A.Semen Persicae is ground
Pulverizing is broken, cross 200 mesh sieves, and then with the hot water extraction 3 times of 72 DEG C, each hot water addition is 7 times of Semen Persicae quality,
Extraction time is 2.2h, merges lixiviating solution, is concentrated into the 22% of original volume, prepares concentrated solution B.Radix Clematidis is ground,
Cross 100 mesh sieves, be subsequently adding the vinegar of its quality 1.1 times, at 72 DEG C, toast 1.1h, be subsequently adding its quality 9 times
Water, intense fire boils, slow fire boiling 1.4h;Filtrate is taken after filtration.By concentrated solution A, concentrated solution B, filtrate mixing, heat up
To 88 DEG C, and isothermal holding 0.6h at such a temperature, it is subsequently adding Oleum sesami, and at 62 DEG C, carries out supersound process 0.6h,
Sonification power is 900W, is condensed into the extractum that relative density is 1.15 and i.e. obtains Chinese medicine preparation.
Embodiment 4
In the embodiment of the present invention, the Chinese medicine preparation of a kind of speckle dispelling, it is made up of according to the raw material of weight portion following: Oleum sesami 8 parts,
A.pusilla A.DC. 24 parts, Radix Clematidis 14 parts, 16 parts of Semen Persicae, Pseudobulbus Pholidotae Chinensis 16 parts, Fructus Bruceae 6 parts.
A.pusilla A.DC., Pseudobulbus Pholidotae Chinensis, Fructus Bruceae are pulverized, crossed 100 mesh sieves, adds the dehydrated alcohol of three's mass 13 times,
62 DEG C of lower seal isothermal holding 1.8h, then carry out supersound process 1.1h at 62 DEG C, and sonification power is 900W, super
Carry out centrifugation after sonication, take supernatant, be then concentrated into the 22% of original volume, prepare concentrated solution A.Semen Persicae is ground
Pulverizing is broken, cross 200 mesh sieves, and then with the hot water extraction 3 times of 72 DEG C, each hot water addition is 7 times of Semen Persicae quality,
Extraction time is 2.2h, merges lixiviating solution, is concentrated into the 22% of original volume, prepares concentrated solution B.Radix Clematidis is ground,
Cross 100 mesh sieves, be subsequently adding the vinegar of its quality 1.1 times, at 72 DEG C, toast 1.1h, be subsequently adding its quality 9 times
Water, intense fire boils, slow fire boiling 1.4h;Filtrate is taken after filtration.By concentrated solution A, concentrated solution B, filtrate mixing, heat up
To 88 DEG C, and isothermal holding 0.6h at such a temperature, it is subsequently adding Oleum sesami, and at 62 DEG C, carries out supersound process 0.6h,
Sonification power is 900W, is condensed into the extractum that relative density is 1.15 and i.e. obtains Chinese medicine preparation.
Embodiment 5
In the embodiment of the present invention, the Chinese medicine preparation of a kind of speckle dispelling, it is made up of according to the raw material of weight portion following: Oleum sesami 7 parts,
A.pusilla A.DC. 22 parts, Radix Clematidis 12 parts, 14 parts of Semen Persicae, Pseudobulbus Pholidotae Chinensis 14 parts, Fructus Bruceae 5 parts.
A.pusilla A.DC., Pseudobulbus Pholidotae Chinensis, Fructus Bruceae are pulverized, crossed 100 mesh sieves, adds the dehydrated alcohol of three's mass 13 times,
62 DEG C of lower seal isothermal holding 1.8h, then carry out supersound process 1.1h at 62 DEG C, and sonification power is 900W, super
Carry out centrifugation after sonication, take supernatant, be then concentrated into the 22% of original volume, prepare concentrated solution A.Semen Persicae is ground
Pulverizing is broken, cross 200 mesh sieves, and then with the hot water extraction 3 times of 72 DEG C, each hot water addition is 7 times of Semen Persicae quality,
Extraction time is 2.2h, merges lixiviating solution, is concentrated into the 22% of original volume, prepares concentrated solution B.Radix Clematidis is ground,
Cross 100 mesh sieves, be subsequently adding the vinegar of its quality 1.1 times, at 72 DEG C, toast 1.1h, be subsequently adding its quality 9 times
Water, intense fire boils, slow fire boiling 1.4h;Filtrate is taken after filtration.By concentrated solution A, concentrated solution B, filtrate mixing, heat up
To 88 DEG C, and isothermal holding 0.6h at such a temperature, it is subsequently adding Oleum sesami, and at 62 DEG C, carries out supersound process 0.6h,
Sonification power is 900W, is condensed into the extractum that relative density is 1.15 and i.e. obtains Chinese medicine preparation.
Comparative example 1
In addition to not containing A.pusilla A.DC., its formula and preparation process are consistent with embodiment 5.
Comparative example 2
Containing only A.pusilla A.DC., its preparation process is consistent with embodiment 5.
Experimental example 6
With the Chinese medicine preparation of embodiment 5 as experimental subject, its whitening and speckle dispelling effect by animal experiment study.
1. the preparation of mice B-16 melanoma cells is by primary cell strain with 0.25% trypsinization 5-8min, adds
RP.MI 1640 culture medium, is separately added into Ka Shi culture bottle Secondary Culture, is prepared as cell suspension standby after treating cell confluency.
2. the cell suspension of preparation is adjusted cell concentration by the mensuration (mtt assay) of cell proliferation is 5x104/ ml, adds 96 hole trainings
Support plate, every hole 100 μ L, then be separately added into variable concentrations medicine (final concentration of 0.50,1.00,2.00,3.00,4.00mg ml-1),
Each concentration sets up 3 multiple holes, averages.And to arrange culture fluid be matched group.Culture plate cell culture incubator will be cultivated
64h, before terminating, 4h adds MTT liquid (5mg ml-1) 10 μ l/ holes, continue to cultivate 5h, with 0.05mol L-1Hydrochloric acid is different
Propanol (100 μ L/ hole) solution terminates reaction, concussion, puts microplate reader and surveys A value, and wavelength is 570nm.The results are shown in Table 1.Knot
Fruit finds that Chinese medicine preparation group has inhibitory action compared with blank group, and optimal inhibition concentration is 1.00g ml-1, Chinese medicine is described
Preparation has obvious inhibiting effect to melanoma cells.
Table 1 mtt assay measures trap A value and suppression ratio
3. tyrosinase activity measures and the cell suspension of preparation adds 96 well culture plates, and every hole 100 μ l adds containing Chinese medicine system
Agent 1.00mg ml-1(15 μ l/ hole) and 2.00mg ml-1Abandoning culture medium after (30/ hole) culture medium culturing 48h, every hole adds
1%TritonX-100 solution 50 μ l, puts into rapidly-30 DEG C of frozen 1h of cryogenic refrigerator, and room temperature thawing subsequently makes cell the most broken
Split, add 1%L-DOPA solution (100 μ L/ hole), 37 DEG C of reaction 2h after 37 DEG C of pre-temperature, measure and absorb angle value, with culture medium
Liquid is matched group.Each concentration sets up 3 multiple holes, averages.The results are shown in Table 2.Table 2 illustrates, Chinese medicine preparation is being made
Obvious inhibitory action is had by activity to tryrosinase after 48 hours, and at 1.00mg ml-1Concentration under act on and being better than
2.00mg·ml-1。
The table 2 impact (A value) on tyrosinase activity
The most intracellular melanin content measures and cell suspension is added culture plate, every hole 1ml, sets up 3 multiple holes, then every hole
Being separately added into Chinese medicine 10,50,100 μ L, with culture fluid matched group.Culture plate is put and cultivates 4d, training of inclining with incubator
Support base, wash 2 times with normal saline, 0.25% trypsinization, collect cell, then process cell with Ultrasonic Pulverization instrument,
Centrifugal, precipitation absolute ethanol washing, centrifugal 5min, incline supernatant, is eventually adding 1mol L-1Sodium hydroxide 100 μ L
After 80 DEG C of water bath heat preservation 2h, survey A value in 470nm, average.The results are shown in Table 3.Show after Chinese medicine preparation effect thin
Intracellular melanocyte substantially reduces.
The A value of table 3 intracellular melanin content
Embodiment 7 acute toxicity maximum resistance test
The Chinese medicine preparation that according to embodiments of the present invention prepared by 1-5 carries out acute toxicity maximum resistance test in animal body, to little
The day maximum tolerated dose that Mus coating is administered is 420 times of the clinical consumption per day of adult, does not observes that the present invention provides under this dosage
The toxic reaction of Chinese medicine preparation made of preparation method.
Embodiment 8 long term toxicity test
The Chinese medicine preparation that according to embodiments of the present invention prepared by 1-5 carries out 6 months long term toxicity tests in animal body, gives every day
Rat coating is administered, and dosage is respectively equivalent to 100 times, 50 times, 25 times of adult's clinic consumption per day every day.Observation item
Include General observation and the complete detection that includes including hematological examination 29.Result shows, three dosages are the most not
Obvious adverse reaction occurs, shows that the Chinese medicine preparation that according to embodiments of the present invention prepared by 1-5 is safe for Clinical practice.
Embodiment 9 long-term clinical trials
The Chinese medicine preparation that embodiment of the present invention 1-5 for oral administration provides, it is possible to the problem significantly improving skin chloasma and black naevus.
Chinese medicine preparation prepared by embodiment of the present invention 1-5 for oral administration and comparative example 1-2, primary coating medicine thickness 0.3-0.5mm,
Once-a-day, it serve on one week, clinical observation, do not find obvious adverse reaction.Curative effect determinate standard is with reference to " new drug clinic is ground
Study carefully guideline " version performed 2002 years.
Curative effect determinate standard:
Basic cure: naked eyes disappear > 90% depending on mottle area, and color disappears substantially, and skin is the most glossy, and black naevus dispels area
> 90%, point system declines index >=0.8 after calculating treatment;
It is effective: naked eyes disappear > 60% depending on mottle area, and color is the most thin out, and skin is more glossy, and black naevus dispels area > 60%,
Point system declines index >=0.5 after calculating treatment;
Take a turn for the better: naked eyes disappear > 30% depending on mottle area, and color is thin out, and skin is glossy, and black naevus dispels area > 30%, comments
Index >=0.3 is declined after calculation of group dividing treatment;
Invalid: naked eyes disappear depending on mottle area, and < 30%, color change is inconspicuous, and black naevus dispels area, and < 30%, point system calculates
Index >=0 is declined after treatment;
Effective percentage=(substantially curing number of cases+effective number of cases+improvement number of cases)/total number of cases * 100%
Obvious effective rate=(substantially curing number of cases+effective number of cases)/total number of cases * 100%
Methods of marking and standard are shown in Table 4:
Table 4
Methods of marking declines the computational methods of index:
Decline score value * 100% before index=(score value after score value one treatment before treatment)/treatment
State of an illness scoring data and Comparison of therapeutic use SPSS statistical software to carry out statistical procedures, use X 2 test, with
P < 0.05 has statistical significance for significant difference, curative effect such as table 5-6.
Table 5 chloasma therapeutic outcome
Group | Total number of cases | Recovery from illness | Effective | Take a turn for the better | Invalid | Obvious effective rate % | Effective percentage % |
Embodiment 1 | 100 | 47 | 38 | 14 | 1 | 85 | 99 |
Embodiment 2 | 100 | 49 | 37 | 13 | 1 | 86 | 99 |
Embodiment 3 | 100 | 78 | 18 | 4 | 0 | 96 | 100 |
Embodiment 4 | 100 | 83 | 12 | 5 | 0 | 95 | 100 |
Embodiment 5 | 100 | 95 | 5 | 0 | 0 | 100 | 100 |
Comparative example 1 | 100 | 6 | 15 | 30 | 49 | 21 | 51 |
Comparative example 2 | 100 | 0 | 1 | 15 | 84 | 1 | 16 |
Table 6 black naevus therapeutic outcome
Clinical therapeutic efficacy shows, embodiment of the present invention 1-5 provide Chinese medicine preparation treatment chloasma obvious effective rate 95% with
On, wherein embodiment 3-5 is preferable, and obvious effective rate is more than 95%, and embodiment 5 is optimum.Dispel the obvious effective rate of black naevus 84%
Above, wherein embodiment 3-5 is preferable, and obvious effective rate is more than 95, and embodiment 5 is optimum.Embodiment 5 and comparative example 1-2 phase
Relatively, it is known that, the present invention is performance effect under the synergism of each raw material.
Model case
Case 1: Wang, female, 28 years old, time conceived, face length had pregnant occasion speckle, used a lot of speckle dispelling product after production, put effect
Undesirable, coating the embodiment of the present invention 5 Chinese medicine preparation after one week cyasma thin out, use after two months cyasma disappear, skin
Skin gloss white wise man.
Case 2: Lee, female, 35 years old, face is grown the concurrent chloasma of freckle, a lot of speckle dispelling product of the black Huang of skin of face,
Effect is undesirable, and the coating embodiment of the present invention 4 Chinese medicine preparation one week, freckle, chloasma disappear, and blee bleaches.
Case 3: Song, female, long on the face have comedo and dust, uses multiple product for resolving poxes to lose effect.Rear coating is originally
Inventive embodiments 3 Chinese medicine preparation after 7 days comedo start to disappear, and do not continue to long;After 10 days, substantially disappear;After again
Continuously coating 10 days, nowadays skin gloss white wise man, then without any speckle, acne etc..
Case 4: health, man, suffers from ruffian.With embodiment 5 Chinese medicine preparation one week, black naevus knot was crazy about the self falling when 7 days,
Red marks after coming off recovers normal after two weeks.Without recurrence when following up a case by regular visits to after 1 year.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, and do not carrying on the back
In the case of the spirit or essential attributes of the present invention, it is possible to realize the present invention in other specific forms.Therefore, no matter from
From the point of view of which point, all should regard embodiment as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all by fall in the implication of equivalency and scope of claim
Change is included in the present invention.
Although moreover, it will be appreciated that this specification is been described by according to embodiment, but the most each embodiment only comprises
One independent technical scheme, this narrating mode of description is only the most for clarity sake, and those skilled in the art should be by
Description is as an entirety, and the technical scheme in each embodiment can also be through appropriately combined, and forming those skilled in the art can
With other embodiments understood.
Claims (5)
1. the Chinese medicine preparation of a speckle dispelling, it is characterised in that be made up of according to the raw material of weight portion following: Oleum sesami 3-10 part,
A.pusilla A.DC. 18-26 part, Radix Clematidis 8-16 part, Semen Persicae 10-18 part, Pseudobulbus Pholidotae Chinensis 10-18 part, Fructus Bruceae 1-8 part.
The Chinese medicine preparation of speckle dispelling the most according to claim 1, it is characterised in that by the following raw material group according to weight portion
Become: Oleum sesami 5-8 part, A.pusilla A.DC. 20-24 part, Radix Clematidis 10-14 part, Semen Persicae 12-16 part, Pseudobulbus Pholidotae Chinensis 12-16 part,
Fructus Bruceae 3-6 part.
The Chinese medicine preparation of speckle dispelling the most according to claim 2, it is characterised in that by the following raw material group according to weight portion
Become: Oleum sesami 7 parts, A.pusilla A.DC. 22 parts, Radix Clematidis 12 parts, 14 parts of Semen Persicae, Pseudobulbus Pholidotae Chinensis 14 parts, Fructus Bruceae 5 parts.
4. the preparation method of the Chinese medicine preparation of the speckle dispelling as described in claim 1-3 is arbitrary, it is characterised in that by following
Step forms:
1) A.pusilla A.DC., Pseudobulbus Pholidotae Chinensis, Fructus Bruceae are pulverized, are crossed 100 mesh sieves, add the dehydrated alcohol of three's mass 12-14 times,
At 60-65 DEG C of lower seal isothermal holding 1.5-2h, at 60-65 DEG C, then carry out supersound process 1-1.2h, sonification power
For 900W, carry out centrifugation after supersound process, take supernatant, be then concentrated into the 20-25% of original volume, prepare and concentrate
Liquid A;
2) Semen Persicae ground, cross 200 mesh sieves, then by the hot water extraction 3 times of 70-75 DEG C, each hot water addition
For 6-8 times of Semen Persicae quality, extraction time is 2-2.5h, merges lixiviating solution, is concentrated into the 20-25% of original volume, prepares dense
Contracting liquid B;
3) Radix Clematidis ground, cross 100 mesh sieves, be subsequently adding the vinegar of its quality 1-1.2 times, at 70-75 DEG C
Baking 1-1.2h, is subsequently adding the water of its quality 8-10 times, and intense fire is boiled, slow fire boiling 1.2-1.5h;Filtrate is taken after filtration;
4) by concentrated solution A, concentrated solution B, filtrate mixing, it is warming up to 85-90 DEG C, and isothermal holding 0.5-0.8h at such a temperature,
Being subsequently adding Oleum sesami, and carry out supersound process 0.5-0.8h at 60-65 DEG C, sonification power is 900W, is condensed into
Relative density be 1.15 extractum i.e. obtain Chinese medicine preparation.
5. the application in treatment medicine for removing speckle of the Chinese medicine preparation as described in claim 1-3 is arbitrary.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610377077.XA CN105853743A (en) | 2016-05-31 | 2016-05-31 | Traditional Chinese medicine preparation for removing chloasma as well as preparation method and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610377077.XA CN105853743A (en) | 2016-05-31 | 2016-05-31 | Traditional Chinese medicine preparation for removing chloasma as well as preparation method and application thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN105853743A true CN105853743A (en) | 2016-08-17 |
Family
ID=56642824
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610377077.XA Pending CN105853743A (en) | 2016-05-31 | 2016-05-31 | Traditional Chinese medicine preparation for removing chloasma as well as preparation method and application thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105853743A (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103142856A (en) * | 2013-03-11 | 2013-06-12 | 温志民 | Traditional Chinese medicine whitening and freckle removing liquid |
CN103599455A (en) * | 2013-10-21 | 2014-02-26 | 张霞 | Traditional Chinese medicine capsule for removing freckle |
CN103610926A (en) * | 2013-11-27 | 2014-03-05 | 成都大医精诚投资管理有限公司 | Chinese medicinal anti-freckle composition, Chinese medicinal anti-freckle preparation and Chinese medicinal anti-freckle mask |
CN103690609A (en) * | 2013-12-31 | 2014-04-02 | 南方医科大学 | Traditional Chinese medicine combination for whitening skin and removing freckles and preparing method of traditional Chinese medicine combination |
CN104208411A (en) * | 2014-08-08 | 2014-12-17 | 兰州古驰生物科技有限公司 | Traditional Chinese medicine preparation for removing yellow spot |
-
2016
- 2016-05-31 CN CN201610377077.XA patent/CN105853743A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103142856A (en) * | 2013-03-11 | 2013-06-12 | 温志民 | Traditional Chinese medicine whitening and freckle removing liquid |
CN103599455A (en) * | 2013-10-21 | 2014-02-26 | 张霞 | Traditional Chinese medicine capsule for removing freckle |
CN103610926A (en) * | 2013-11-27 | 2014-03-05 | 成都大医精诚投资管理有限公司 | Chinese medicinal anti-freckle composition, Chinese medicinal anti-freckle preparation and Chinese medicinal anti-freckle mask |
CN103690609A (en) * | 2013-12-31 | 2014-04-02 | 南方医科大学 | Traditional Chinese medicine combination for whitening skin and removing freckles and preparing method of traditional Chinese medicine combination |
CN104208411A (en) * | 2014-08-08 | 2014-12-17 | 兰州古驰生物科技有限公司 | Traditional Chinese medicine preparation for removing yellow spot |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103961636B (en) | Orthopedic disease treatment external application traditional Chinese medicine composition, preparation method and application thereof | |
CN104524335B (en) | A kind of pill and preparation method for female endocrine dyscrasia conditioning | |
CN102784244B (en) | Externally applied traditional Chinese medicine for treating breast lumps | |
CN103736017A (en) | Traditional Chinese medicine composition for treating intercostal neuralgia and preparation method of traditional Chinese medicine composition | |
CN104436145B (en) | It is a kind of to prevent and/or treatment allergic rhinitis and the pharmaceutical composition of allergic asthma | |
CN103027978A (en) | Medicine composition for treating traumatic injury and preparation method of medicine composition | |
CN104147540A (en) | Traditional Chinese medicine composition for treating malignant tumor | |
CN104324081A (en) | Corydalis tuber superfine powder and preparation method and application thereof | |
CN105853743A (en) | Traditional Chinese medicine preparation for removing chloasma as well as preparation method and application thereof | |
CN106039174A (en) | Traditional Chinese medicinal liquor for treating osteoarthrosis | |
CN110478416A (en) | The composition and its preparation method and application for treating rheumatic rheumatoid arthritis | |
CN105343660A (en) | Traditional Chinese medicinal preparation for treating peripheral vertigo and preparation method of traditional Chinese medicinal preparation | |
CN101690746B (en) | Chinese herbal preparation for treating soft tissue injuries and fractures | |
CN105147956A (en) | Traditional Chinese medicine for treating acute peptic ulcer bleeding and preparation method of traditional Chinese medicine | |
CN104758358A (en) | Medicine composite used for eczema and preparation method thereof | |
CN104225259A (en) | Traditional Chinese medicine for treating stagnant heat type angiitis and preparation method thereof | |
CN104706562B (en) | A kind of Chinese herbal medicine whitening cream and preparation method thereof | |
CN103877557B (en) | A kind of Chinese medicine ointment and preparation method for the treatment of tinea unguium | |
CN103655822B (en) | Anti-tumor drug as well as preparation method and application thereof | |
CN103690902B (en) | For preventing the Chinese medicine preparation of nasopharyngeal carcinoma radiation side reaction | |
CN106237004A (en) | A kind of Integrated TCM preparation treating lichen chronicus simplex and preparation method | |
CN105288016B (en) | External traditional Chinese medicine emulsion for preventing and treating knee osteoarthritis and preparation method thereof | |
CN104689260B (en) | It is a kind of to treat breast cancer and the Chinese medicine preparation of endocrinotherapy for breast cancer side effect be improved | |
CN105998218A (en) | Pharmaceutical composition for treating female chloasma | |
CN104116795B (en) | A kind of have Chinese medicine composition of anti-skin carcinoma activity and uses thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160817 |