CN105850267B - Improve the method that alfalfa seed is sprouted under acid-aluminum stress - Google Patents
Improve the method that alfalfa seed is sprouted under acid-aluminum stress Download PDFInfo
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- CN105850267B CN105850267B CN201610221593.3A CN201610221593A CN105850267B CN 105850267 B CN105850267 B CN 105850267B CN 201610221593 A CN201610221593 A CN 201610221593A CN 105850267 B CN105850267 B CN 105850267B
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- alfalfa seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Environmental Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The method that alfalfa seed is sprouted under acid-aluminum stress is improved the invention discloses a kind of, is related to grass industry and administrative skill field.This method is：1. being sterilized to alfalfa seed, at ambient temperature, alfalfa seed is soaked in 0.15% liquor potassic permanganate and sterilizes 5min；2. with epiphysin solution seed soaking, alfalfa seed is impregnated into 8h under the conditions of room temperature is protected from light；3. seed is promoted to sprout, 25 ± 1 DEG C are placed in, cultivates 10 days in 10h illumination/14h dark seed is promoted to sprout.The epiphysin that the present invention uses is a kind of amine bormones, nontoxic, and dosage is few, economical when handling seed, simple and practicable, quick, operability is strong, can improve the sprouting quality of alfalfa seed in a short time；It can effectively solve the problems, such as that alfalfa seed germination rate in acid soil is low；The popularizing planting for being alfalfa in acid soil in the south provides technical support.
Description
Technical field
The present invention relates to alfalfa kinds under grass industry and administrative skill field more particularly to a kind of raising acid-aluminum stress
The method that son is sprouted.
Background technology
Acid soil refers to the soil that pH value is less than 7, and for agricultural production, topsoil pH value is less than 6.6
As acid soil.Acid soil account for the whole world can farming soil 40%, account for about the 21% of land area in China, it is widely distributed
In southern Red-yellow, and as the area of the deterioration acid soil of environment is constantly increasing.Aluminium is that content is most rich in the earth's crust
Rich metallic element, accounts for 7% of earth crustal mass or so.Under field conditions (factors), it mainly exists in the form of aluminosilicate, and biology can
Usability very little.Soil acidification is made due to the use of agrochemical and Acid Rain Effect in recent years, a large amount of indissolubles in acid soil
The aluminium of state is dissolved out with ionic state, and activated state is presented, and soluble aluminum concentration rises, and destroys the Physiology and biochemistry in crops body
Process, it is suppressed that the growth of crop reduces crop yield.Therefore, it is raw to have become the whole world for crop aluminium poison caused by soil acidification
Object and the hot spot of environmental science concern.
Alfalfa is that a kind of pulse family clover belongs to perennial high quality forage, and well developed root system, main root is coarse, has stronger resistance to
Cold, drought resisting and power of regeneration, nutritive value is high, and palatability is good, and various livestocks all compared with eating, are known as " King of Pasture ".According to
The ﹪ and lysine content 1.05-1.38% of 15-25 containing thick protein in alfalfa dry matter are measured, it is higher 1- than corn respectively
1.5 times and 4-5 times.Alfalfa is both the important plant improved the crop of soil and conserved water and soil.The root system of alfalfa
It buries deep and broad, rhizobium can fix the nitrogen in air, dramatically increase soil nitrogenous amount；Moreover it is possible to it stays in the soil big
Root stubble is measured, increases a large amount of organic matters for soil, Soil building improves the yield of succession crop.Alfalfa has very long flower
Phase and a kind of important nectariferous plant.
Due to the development of domestic animal husbandry, the cultivated area of China clover constantly expands in recent years, but is concentrated mainly on north
Side area, and southern area cultivated area is relatively fewer.To find out its cause, it is more sensitive to acid mainly due to alfalfa, be in pH
Soil-grown between 6.5-7.5 is convenient, and when soil pH is less than 6.0, the growing way of alfalfa is affected, and causes
A large amount of rhizobium normally cannot breed and survive.When soil pH is less than 4.5, alfalfa normal growth is heavily suppressed,
Plant death is even resulted in, therefore, it is difficult to plantation is widely applied on acid soil.Recently as southern area animal husbandry
Fast development, especially dairy fast development, the demand to high protein high quality forage alfalfa also gradually increases, but
Clover has seriously affected plantation of the alfalfa in southern area to acid soil sensitivity.Seed sprouting is in plant life history
Crucial transition stage (from seed to seedling), it decides the living environment faced after plant germination and Natural selection pressure
Power.Influence initial reaction of the activated aluminum horizontal and higher the relatively low pH of acid soil to clover is to inhibit sprouting for alfalfa seed
Hair and seedling growth and development, and then inhibit clover root system growth, and thus inhibit moisture, nutrient absorption, for a long time
Acid-Al stress can cause aerial growth to be obstructed.
The alumite ability required period that clover is improved using breeding technique is long, is difficult in adapt to clover in a short time in south
The needs of square acid soil popularizing planting and animal husbandry development.By reasonable employment allogenic material, with reference to management such as cultivation managements
Measure improves the alumite ability of clover, has the features such as easy to operate, quick, is easily accepted by the public, have it is wide should
Use prospect.
Invention content
The influence sprouted the purpose of the present invention is being directed to acid-aluminum stress to alfalfa seed provides a kind of acid aluminium that improves and coerces
Compel the method that lower alfalfa seed is sprouted.
The object of the present invention is achieved like this：
The sprouting ability of alfalfa seed is effectively improved in a short time using epiphysin solution seed soaking, to give full play to
China acid soil area has abundant hydrothermal condition to carry out leguminous forage production, for China acid soil area scale
Changing development herbage and animal husbandry industry all has very high realistic meaning.
Gather specifically, improving the method (abbreviation method) that alfalfa seed is sprouted under acid-aluminum stress including following step：
1. alfalfa seed is sterilized
At ambient temperature, alfalfa seed is soaked in 0.15% liquor potassic permanganate and sterilizes 5min, then
Distilled water flushing is used after seed is removed filter dry moisture 3 times, is dried；
2. with epiphysin solution seed soaking
Epiphysin is made into the optium concentration of 20 μm of ol/L, alfalfa seed is impregnated into 8h under the conditions of room temperature is protected from light,
Then it removes filter dry；
3. the sprouting of alfalfa seed
Two layers of filter paper is layed in culture dish, with pH value 4.5 and to soak filter paper containing 100 μm of aluminum ions solution of ol/L
Wet, the alfalfa seed being filtered dry after then epiphysin is impregnated uniformly is sowed on filter paper, is placed in 25 ± 1 DEG C, 10h illumination/14h
Being cultivated 10 days in dark promotes seed to sprout.
The present invention has following advantages and good effect：
1. epiphysin is a kind of amine bormones, nontoxic, dosage is few, economical when handling seed, simple and practicable, take effect
Soon, operability is strong, can improve the sprouting quality of alfalfa seed in a short time；
2. it can effectively solve the problems, such as that alfalfa seed germination rate in acid soil is low；
3. the popularizing planting for being alfalfa in acid soil in the south provides technical support.
Description of the drawings
Fig. 1 is influence coordinate diagram of the epiphysin to alfalfa seed germination percentage under acid-aluminum stress；
Fig. 2 is influence coordinate diagram of the epiphysin to alfalfa seed germinating energy under acid-aluminum stress.
Specific embodiment
It is described in detail with reference to embodiment and attached drawing：
First, embodiment 1
Epiphysin processing improves alfalfa seed germination test under acid-aluminum stress under manual control environment, and key step is such as
Under：
1st, alfalfa seed is sterilized
It is material to select 995 ' seeds of commercial alfalfa cultivars ' huge energy.Alfalfa seed is soaked in and is equipped with
5min is sterilized in the beaker of 0.15% liquor potassic permanganate, then removes filter dry seed, with being dried after distilled water flushing 3 times.
2nd, optimal use concentration and soaking time determine
To determine the optium concentration and optimal soak time of epiphysin solution, epiphysin solution is set into 0,10,20,40 and
Soaking time is set 0,4,8 and 12h, tetra- soaking time gradients, seed to be pulled out after impregnating by 80 μm of ol/L, five concentration gradients
It is filtered dry；Two layers of filter paper is layed in culture dish, with pH value 4.5 and to soak filter paper containing 100 μm of aluminum ions solution of ol/L,
Then the alfalfa seed being filtered dry after epiphysin is impregnated uniformly is sowed on filter paper, be placed in 25 ± 1 DEG C, 10h illumination/14h it is black
Cultivating 10 days in the dark promotes seed to sprout, and counts the germination of alfalfa seed daily；The result shows that clover kind under acid-aluminum stress
The germination percentage and germinating energy of son are significantly affected, such as table 1, table 2.
The influence (%) of table 1 different epiphysin concentration and seed soaking time to percentage of seedgermination
The influence (%) of table 2 different epiphysin concentration and seed soaking time to potentiality of seed
It is significantly improved under acid-aluminum stress than unsoaked percentage of seedgermination through the alfalfa seed that epiphysin solution impregnated,
And different seed soaking times promotes the effect that seed is sprouted also different, wherein to be soaked under the solution concentration of 20 μm of ol/L
The kind percentage of seedgermination of 8h and germinating energy highest show the epiphysin solution seed soaking 8h of 20 μm of ol/L to improving alfalfa seed
Sprouting effect under acid-aluminum stress is best.
2nd, embodiment 2
Epiphysin processing improves alfalfa seed germination test under acid-aluminum stress under the natural environment of crop field, and operating procedure is such as
Under：
1st, alfalfa seed is sterilized
It is material to select 995 ' seeds of commercial alfalfa cultivars ' huge energy.Alfalfa seed is soaked in and is equipped with
5min is sterilized in the beaker of 0.15% liquor potassic permanganate, then removes filter dry seed, with being dried after distilled water flushing 3 times.
2nd, with epiphysin solution seed soaking
Epiphysin is made into the best solution concentration of 20 μm of ol/L, alfalfa seed is soaked seed under the conditions of room temperature is protected from light
Then 8h is removed filter dry；
3rd, it sows：
It is experimental field yellow earth, pH value is 5.5~6.0.Ground is ploughed deeply before sowing, applies 35kg/ mus of calcium superphosphate and carbonic acid
45kg/ mus of hydrogen ammonium is as base manure, then with mechanical rotary tillage, and with 3kg/ mus of 3% Furadan, 75% fenaminosulf wettable powder
1.5kg/ even spread fertilizer over the fields in soil per mu carries out disinfection, then that ground is smooth while spray preemergence herbicide；September part is broadcast in the fall
Kind, 995 ' seed broadcastings of alfalfa commercial breed ' huge energy are selected, every square metre of 1.0g after planting covers fine earth about 0.5cm.It broadcasts
It is kind initial stage, diligent to water, ground moistening is kept to germinate with sharp；In 10 days after planting, germination situation, statistics hair are investigated daily
Bud rate and germinating energy；The result shows that：As shown in Figure 1, Figure 2, in the acid yellow soil for being 5.5~6.0 or so in pH, alfalfa seed
It germinates significantly affected, shows as germination percentage and germinating energy declines；But the epiphysin solution through 20 μm of ol/L is kept away in room temperature
Alfalfa seed is soaked seed 8h under the conditions of light, significantly improves its germination percentage and germinating energy.
Claims (1)
1. a kind of improve the method that alfalfa seed is sprouted under acid-aluminum stress, it is characterised in that：
1. alfalfa seed is sterilized
At ambient temperature, alfalfa seed is soaked in 0.15% liquor potassic permanganate and sterilizes 5min, then by seed
Distilled water flushing is used after removing filter dry moisture 3 times, is dried；
2. with epiphysin solution seed soaking
Epiphysin is made into the optium concentration of 20 μm of ol/L, alfalfa seed is impregnated into 8h under the conditions of room temperature is protected from light, then
It removes filter dry；
3. seed is promoted to sprout
Two layers of filter paper is layed in culture dish, with pH value 4.5 and to soak filter paper containing 100 μm of aluminum ions solution of ol/L,
Then the alfalfa seed being filtered dry after epiphysin is impregnated uniformly is sowed on filter paper, be placed in 25 ± 1 DEG C, 10h illumination/14h it is black
Cultivating 10 days in the dark promotes seed to sprout.
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| CN106258079B (en) * | 2016-08-19 | 2019-11-08 | 四川农业大学 | A kind of Kiwi berry seedling breeding method |
| CN106922515A (en) * | 2017-05-09 | 2017-07-07 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of indoor fast breeding method of waste vetch |
| CN108713368B (en) * | 2018-05-29 | 2021-03-02 | 湖南农业大学 | Method for promoting germination of alfalfa seeds and healthy growth of root systems under acid copper stress |
| CN110999592A (en) * | 2020-02-11 | 2020-04-14 | 山西农业大学 | Device and method for concentrated sulfuric acid delinting and vitality restoration of white sheep grass seeds |
| CN112449790A (en) * | 2020-12-11 | 2021-03-09 | 菏泽学院 | Method for improving salt stress resistance of lawn grass germination by using salicylic acid-soaked seeds |
| CN115280932B (en) * | 2022-09-15 | 2023-11-24 | 西北农林科技大学 | Seed initiation method for improving salt tolerance of alfalfa |
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| CN104604864A (en) * | 2015-02-13 | 2015-05-13 | 中国农业大学 | Melatonin solution capable of improving anti-stress capability of plant |
| CN104823738A (en) * | 2015-04-13 | 2015-08-12 | 浙江大学 | Application of melatonin in reducing cadmium accumulation in plants |
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