CN105850267A - Method for improving germination of medicago sativa seeds under acid-aluminum stress - Google Patents

Abstract

The invention discloses a method for improving germination of medicago sativa seeds under acid-aluminum stress and relates to the technical field of production and management forage grass. The method comprises the following steps: (1) disinfecting the medicago sativa seeds, namely immersing the medicago sativa seeds into a 0.15% potassium permanganate solution under a room-temperature condition for disinfecting for 5 minutes; (2) immersing the seeds in a melatonin solution, namely immersing the medicago sativa seeds under a room-temperature dark condition for 8 hours; and (3) promoting the germination of the seeds, namely culturing the seeds under the conditions that the temperature is 25+/-1 DEG C and the seeds are illuminated for 10 hours and then are put into a dark place for 14 hours alternately for 10 days to promote the germination of the seeds. Melatonin used by the invention is an amine type hormone and has no toxin and harms; the dosage of the melatonin is less when the melatonin is used for treating the seeds so that the treatment is economical; the method is simple and feasible, has rapid effect and is strong in operability; the germination quality of the medicago sativa seeds can be improved in short time; the problem that the germination rate of the medicago sativa seeds in acidic soil is low can be effectively solved; and technical supports are provided for popularizing and planting medicago sativa in Southern acid soil.

Description

Improve the method that under acid-aluminum stress, alfalfa seed is sprouted

Technical field

The present invention relates to grass industry and management technique field, particularly relate to alfalfa kind under a kind of raising acid-aluminum stress The method that son is sprouted.

Background technology

Acid ground refers to the pH value soil less than 7, and for agricultural production, topsoil pH value is less than 6.6 It is acid ground.Acid ground account for the whole world can farming soil 40%, account for the 21% of land area in China, be distributed widely in Red-yellow, south, and along with the area deteriorating acid ground of environment is constantly increasing.Aluminum is that in the earth's crust, content is the abundantest Metallic element, account for about the 7% of earth crustal mass.Under field conditions (factors), it mainly exists with aluminosilicate form, and biology can profit The least by property.Soil acidification is made in recent years, a large amount of indissoluble shapes in acid ground due to use and the Acid Rain Effect of agrochemical The aluminum of state, with ionic state dissolution, presents activated state, and soluble aluminum concentration rises, and destroys the Physiology and biochemistry mistake in crops body Journey, it is suppressed that the growth of crop, reduces crop yield.Therefore, it is biological that the crop aluminum poison that soil acidification causes has become the whole world Learn and the focus of environmental science concern.

Alfalfa is a kind of perennial high quality forage of pulse family Medicago, well developed root system, and main root is thick, has stronger resistance to Cold, drought resisting and regeneration capacity, be of high nutritive value, and good palatability, various domestic animals the most relatively eating, is described as " King of Pasture ".According to Measure containing thick protein 15-25 and lysine content 1.05-1.38% in alfalfa dry, higher 1-than Semen Maydis respectively 1.5 times and 4-5 times.Alfalfa is the crop of improvement soil, is also to maintain the important plant of water and soil.The root system of alfalfa Burying deep and broad, its root nodule bacteria can fix the nitrogen in air, dramatically increases soil nitrogenous amount；Moreover it is possible to stay big in soil Amount root stubble, increases a large amount of organic for soil, and Soil building improves the yield of succession crop.Alfalfa has the longest flower Phase, is also a kind of important nectariferous plant.

Due to the development of domestic animal husbandry, the cultivated area of China Herba Medicaginis constantly expands in recent years, but is concentrated mainly on north Area, side, and southern area cultivated area is relatively fewer.Trace it to its cause, more sensitive to acid mainly due to alfalfa, at pH be Conveniently, when soil pH is less than 6.0, the growing way of alfalfa is affected soil-grown between 6.5-7.5, and A large amount of root nodule bacteria are caused can not normally to breed and survive.When soil pH is less than 4.5, alfalfa normal growth is seriously pressed down System, even results in plant dead, therefore, it is difficult to spread plantation on acid ground.Recently as southern area herding The fast development of the fast development of industry, especially dairy, is also gradually increased the demand of high protein high quality forage alfalfa, But Herba Medicaginis is sensitive to acid ground, has had a strong impact on the alfalfa plantation in southern area.Seed germination is plant life history In crucial transition stage (from seed to seedling), living environment that it is faced after decide plant germination and Natural selection pressure Power.PH level and higher activated aluminum that acid ground is relatively low inhibit alfalfa seed to the initial reaction that affects of Herba Medicaginis Sprout and the growth promoter of seedling, and then the growth of suppression Herba Medicaginis root system, and thus suppress moisture, the absorption of nutrient, for a long time Acid-Al stress aerial growth can be caused to be obstructed.

Cycle required for using the alumite ability of breeding technique raising Herba Medicaginis is long, is difficult in adapt to Herba Medicaginis in a short time in south Side's acid ground popularizing planting and the needs of animal husbandry development.By reasonable employment allogenic material, manage in conjunction with cultivation management etc. The features such as measure improves the alumite ability of Herba Medicaginis, has easily operation, instant effect, the most accepted by the public, there is wide answering Use prospect.

Summary of the invention

It is an object of the invention to impact alfalfa seed sprouted for acid-aluminum stress, it is provided that a kind of acid aluminum that improves is coerced Compel the method that lower alfalfa seed is sprouted.

The object of the present invention is achieved like this:

Melatonin solution seed soaking is utilized to be effectively improved the sprouting ability of alfalfa seed in a short time, to China can be given full play to Acid ground area possesses abundant hydrothermal condition to carry out leguminous forage production, sends out for the area scale of China's acid ground Exhibition herbage and animal husbandry industry all have the highest realistic meaning.

Specifically, improve the method (abbreviation method) that under acid-aluminum stress, alfalfa seed is sprouted and include that following step is gathered:

1. alfalfa seed is sterilized

At ambient temperature, alfalfa seed is soaked in the potassium permanganate solution of 0.15% sterilization 5min, then by seed Use distilled water flushing 3 times after removing filter dry moisture, dry；

2. melatonin solution seed soaking is used

Melatonin is made into the optium concentration of 20 μm ol/L, under the conditions of room temperature lucifuge, alfalfa seed is soaked 8h, then Remove filter dry；

3. the sprouting of alfalfa seed

Two layers of filter paper is layed in culture dish, is 4.5 with pH value and filter paper is soaked containing 100 mol/L aluminum ions solution, Then after being soaked by melatonin, the alfalfa seed that is filtered dry uniformly is sowed on filter paper, be placed in 25 ± 1 DEG C, 10h illumination/14h black Cultivate in the dark and promote seed germination in 10 days.

The present invention has following advantages and a good effect:

1. melatonin is a kind of amine bormones, nontoxic, and when processing seed, consumption is few, economical, simple, instant effect, can Strong operability, can improve the sprouting quality of alfalfa seed in a short time；

2. the problem that alfalfa seed germination rate in acid ground is low can effectively be solved；

3. technical support is provided for alfalfa popularizing planting in acid soil in the south.

Accompanying drawing explanation

Fig. 1 be melatonin under acid-aluminum stress alfalfa seed germination percentage affect coordinate diagram；

Fig. 2 be melatonin under acid-aluminum stress alfalfa seed germinating energy affect coordinate diagram.

Detailed description of the invention

Describe in detail below in conjunction with embodiment and accompanying drawing:

One, embodiment 1

Under manual control environment, melatonin processes and improves alfalfa seed sprouting test under acid-aluminum stress, and key step is as follows:

1, alfalfa seed is sterilized

' huge energy 995 ' seed is material to select commercial alfalfa cultivars.Alfalfa seed is soaked in equipped with 0.15% Gao Meng Sterilize in the beaker of acid potassium solution 5min, is then removed filter dry by seed, dries with after distilled water flushing 3 times.

2, optimal concentration and the determination of soak time

For determining the optium concentration of melatonin solution and optimal soak time, melatonin solution is arranged 0,5,10,20 and 40 μ Five Concentraton gradient of mol/L, arrange 0,4,8 and 12h tetra-soak time gradients by soak time, and seed pulls filter out after soaking Dry；Two layers of filter paper is layed in culture dish, is 4.5 with pH value and filter paper is soaked, so containing 100 mol/L aluminum ions solution After melatonin is soaked after the alfalfa seed that is filtered dry uniformly be sowed on filter paper, be placed in 25 ± 1 DEG C, 10h illumination/14h dark Middle cultivation promotes the germination that seed germination, every day add up alfalfa seed for 10 days；Result shows, alfalfa seed under acid-aluminum stress Germination percentage and germinating energy significantly affected, such as table 1, table 2.

The alfalfa seed crossed through melatonin solution soaking at acid-aluminum stress under significantly improve than unsoaked percentage of seedgermination, and Different seed soaking times, it promotes that the effect of seed germination is the most different, wherein with the 8h that soaks seed under the solution concentration of 20 μm ol/L Percentage of seedgermination and germinating energy the highest, show that the melatonin solution seed soaking 8h of 20 μm ol/L exists to improving alfalfa seed Sprouting effect under acid-aluminum stress is best.

Two, embodiment 2

Under the natural environment of land for growing field crops, melatonin processes and improves alfalfa seed sprouting test under acid-aluminum stress, and operating procedure is as follows:

1, alfalfa seed is sterilized

' huge energy 995 ' seed is material to select commercial alfalfa cultivars.Alfalfa seed is soaked in equipped with 0.15% Gao Meng Sterilize in the beaker of acid potassium solution 5min, is then removed filter dry by seed, dries with after distilled water flushing 3 times.

2, melatonin solution seed soaking is used

Melatonin is made into the optimal solution concentration of 20 μm ol/L, and soak seed under the conditions of room temperature lucifuge 8h by alfalfa seed, Then remove filter dry；

3, sowing:

Being experimental field yellow earth, pH value is 5.5～6.0.Before Bo Zhong, ground is ploughed deeply, apply calcium superphosphate 35kg/ mu and ammonium hydrogen carbonate 45 kg/ mus are as base manure, then with machinery rotary tillage, and with 3% Furadan 3kg/ mu, 75% fenaminosulf wettable powder 1.5kg/ even spreading fertilizer over the fields in soil per mu carries out disinfection, and then sprays preemergence herbicide by smooth for ground simultaneously；JIUYUE is broadcast in the fall Kind, ' huge energy 995 ' seed broadcasting, every square metre of 1.0g, after planting cover fine earth about 0.5cm to select alfalfa commercial breed.Broadcast At the initial stage of kind, duty is watered, and keeps ground moistening in order to germination；In 10 days after planting, investigating germination situation every day, statistics is sent out Bud rate and germinating energy；Result shows: as shown in Figure 1, Figure 2, in the acid yellow soil that pH is 5.5～about 6.0, alfalfa seed Germinate significantly affected, show as germination percentage and germinating energy declines；But keep away in room temperature through the melatonin solution of 20 μm ol/L Soak seed under optical condition 8 h by alfalfa seed, significantly improves its germination percentage and germinating energy.

Claims (1)

1. one kind is improved the method that under acid-aluminum stress, alfalfa seed is sprouted, it is characterised in that:

1. alfalfa seed is sterilized

At ambient temperature, alfalfa seed is soaked in the potassium permanganate solution of 0.15% sterilization 5min, then by seed Use distilled water flushing 3 times after removing filter dry moisture, dry；

2. melatonin solution seed soaking is used

Melatonin is made into the optium concentration of 20 μm ol/L, under the conditions of room temperature lucifuge, alfalfa seed is soaked 8h, then Remove filter dry；

3. seed germination is promoted

Two layers of filter paper is layed in culture dish, is 4.5 with pH value and filter paper is soaked containing 100 mol/L aluminum ions solution, Then after being soaked by melatonin, the alfalfa seed that is filtered dry uniformly is sowed on filter paper, be placed in 25 ± 1 DEG C, 10h illumination/14h black Cultivate in the dark and promote seed germination in 10 days.