CN105766314A - Novel method for improving catalpa bungei cuttage propagation efficiency - Google Patents
Novel method for improving catalpa bungei cuttage propagation efficiency Download PDFInfo
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- CN105766314A CN105766314A CN201610154933.5A CN201610154933A CN105766314A CN 105766314 A CN105766314 A CN 105766314A CN 201610154933 A CN201610154933 A CN 201610154933A CN 105766314 A CN105766314 A CN 105766314A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a novel method for improving catalpa bungei cuttage propagation efficiency.The method includes the first step of construction of a cutting orchard, the second step of preparation of a cuttage seedbed, the third step of preparation of a cuttage matrix, the fourth step of cultivation and harvesting of cuttings, the fifth step of cuttage, the sixth step of daily management after cuttage, and the seventh step of seedling hardening and transplanting.According to the transpiration and rooting rules of plants, tender branches low in lignification degree are obtained in a shading mode, cuttings are subjected to shading treatment through a shading method, the catalpa bungei cuttage survival rate is greatly increased and as high as 92%, inconvenience caused by catalpa bungei seedling propagation by means of tissue culture, grafting and other technologies is overcome, nursery cultivation time is greatly shortened, operation is easy, and the method is convenient to control and good in practicality.
Description
Technical field
The invention belongs to Chinese catalpa seedling breeding technical field, be specifically related to a kind of new method improving Chinese catalpa cottage propagation efficiency.
Background technology
Chinese catalpa (Catalpabungei) it is the deciduous tree of Bignoniaceae Catalpa, originate in China, be mainly distributed on the Yellow River and the Yangtze river basin.Chinese catalpa just has begun to large-area plantation in Han dynasty, its preventing erosion, protect the fields, build Timber stands, garden is viewed and admired and has important using value in roadside greening etc..Along with constantly advancing and the requirement of industrial economy development of China's Construction of Eco-urban, the demand for Chinese catalpa improved seeds will constantly expand.But current Chinese catalpa seedling breeding technology still suffers from numerous deficiencies, it is mainly manifested in the following aspects: 1) no matter hard branch or twig, cuttage survival rate is all very low, and what in existing report, effect was best is also only about 50%；2) seed propagation is limited to seed production, and it cannot be guaranteed that the filial generation shape of excellent strain；3) grafting easily occurs that rhizosphere nematode endangers；4) tissue culture is with high costs, and technical sophistication is loaded down with trivial details.Therefore, invent and a kind of can the new method of improved seeds breeding Chinese catalpa rapidly and efficiently in a large number tool be of great significance.
Summary of the invention
Goal of the invention: for the deficiencies in the prior art, it is an object of the invention to provide a kind of new method improving Chinese catalpa cottage propagation efficiency, for improving the efficiency of Chinese catalpa seedling breeding, reduce Chinese catalpa seedling cost, the expanding propagation cycle shortening Chinese catalpa breeding the shape-stable ensureing excellent strain heredity, the factory culturing for Chinese catalpa breeding lays the foundation.
Technical scheme: in order to realize foregoing invention purpose, the technical solution used in the present invention is:
A kind of new method improving Chinese catalpa cottage propagation efficiency, comprises the following steps:
1) foundation of cutting orchard: female parent is the raw seedling of 1-2, in late autumn or field planting in early spring, cuts bar, in order to inspire more side shoot, use sufficient fertilizer the daily management that carries out sheltering from heat or light in liftoff 30cm place after field planting；
2) preparation in cuttage seedbed: seedbed is high with brick one-tenth 20cm, 100cm width, ground, length visual field and fixed substrate grooves, stay the management passage of 60cm between groove；After filling substrate, set up Small plastic shed again, mop floor with surrounding after cover film, can be as the criterion with brick compacting；
3) preparation of cutting medium: peat, perlite, Vermiculitum are stirred according to the volume ratio of 60:20:20, inserts in ready cuttage groove, and thickness 20cm waters permeable before cuttage, water permeable after guarantee more than hypothallus 15cm；
4) cultivation of fringe bar with gather: fringe bar is cultured to late May at the beginning of 6 months, starts first time and adopts fringe, adopts fringe and start to shelter from heat or light for first 10 days, it is ensured that intensity of illumination, less than 8000LX, inspires excessive growth new slightly；Shade net is packed up after adopting fringe, fertilising, water, after new slightly 20cm, enter next one circulation；
5) cuttage: fringe bar is transferred to indoor immediately and cuts into cutting after gathering, base portion otch is immediately inserted into after dipping root-inducing powder in ready seedbed, waters permeable after being plugged, and builds film, seals canopy moisturizing, enters follow-up schedule management link；
6) daily management after inserting: temperature maintains 28 DEG C-35 DEG C, strengthens vegetable active, promotes early Fa Xingen；Opening thin film after 7 days, check seedbed moisture, cutting survives situation, sprays 1000 times of state's light brute force root-growing agents once, again builds；Within 8-10 days, starting to take root, when 20 days, rooting rate is the highest；
7) seedling exercising and transplanting: after cuttage 2 weeks, spot-check rooting rate, when reaching 50%, starts seedling exercising management of leaking informaton；When cutting seedling second time lifts growth, root system is how plentiful, now can transplant.
In step 1), building and set up retractable movable shade, height 2.5m by 4m × 4m or 4m × 6m when cutting orchard is set up, shading rate requires to reach 90-95%.
In step 4), late autumn arranges trench digging buriing farm manure or fertilizer, every 5kg between female parents two after fallen leaves, enter winter forward sight soil moisture content water permeable once；At the beginning of 3 months beginning of springs depending on soil moisture content water permeable once, at the beginning of 5 months leaves launch after buriing carbamide once, 100 grams every, after adopting fringe later every time mend execute once, water depending on soil moisture content；
In step 4), choose mounted blade when adopting fringe, branch that branch is delicate is cut, under the place's of cutting branch answer hands to pinch namely to break, the heart should be solid, green herein.
In step 5), cutting stem segment length's 10-15cm, 1-2 bud, retain full leaf, a cutting slightly can be individually done on top, and base portion otch is immediately inserted into after dipping root-inducing powder in ready seedbed, and cutting density is 10cm × 8cm, is advisable in the slightly unearthed face of the above pulvinus of the degree of depth.
In step 7), the seedling exercising that leaks informaton management particularly as follows: first 3 day daytime normal management, at 5 in afternoon opened shed two to next day 8, ventilation wet down.Within 3 days, for daytime, shed two being opened, ventilation wet down, at 5 in afternoon all opened shed to next day 8, ventilation wet down again.Remove thin film afterwards, water 1 ‰ carbamide rich water once.
Beneficial effect: compared with prior art, the new method improving Chinese catalpa cottage propagation efficiency of the present invention, the technical system bred of epicormic branch cuttage being material with Chinese catalpa twig, cuttage survival rate is high；Can guarantee that the filial generation shape invariance of excellent strain；Cost is low, simple to operate；Shorten the expanding propagation cycle of Chinese catalpa breeding；Adapt to the requirement of the factory culturing of Chinese catalpa breeding seedling, the problem solving the Chinese catalpa large-scale industrialized Cultivating techniques of breeding seedling, reduce production cost, there is good practicality.Show through test in continuous 2 years for many years, the twig using Chinese catalpa carries out cuttage and combines process of sheltering from heat or light accordingly, can so that the cuttage survival rate of Chinese catalpa reaches more than 85%, shorten the expanding propagation cycle of Chinese catalpa breeding, reduce reproductive-cost, simple to operate, and can guarantee that the filial generation shape invariance of excellent strain, factory culturing for Chinese catalpa breeding seedling lays the foundation, and may advantageously facilitate the development of Chinese catalpa industry.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further illustrated.The following stated is only the good embodiment of the present invention, is used only for describing the present invention, it is impossible to be interpreted as the restriction to the scope of the present invention.It should be pointed out that, all any amendment, equivalent replacement and improvement etc. made within the spirit and principles in the present invention, should be included within protection scope of the present invention.
Embodiment 1
A kind of new method improving Chinese catalpa cottage propagation efficiency, comprises the following steps:
1) foundation of cutting orchard: select prepartion of land, irrigation and drainage good, the plot that soil property is fertile, the good sandy loam of drainage is more preferably.By the retractable movable shade of 4m × 6m erection, height about 2.5m, shading rate requires to reach 95%.Apply farm manure by the amount of 4000kg/ mu or fertilizer makes base fertilizer, plough deeply and make ridge, ridge face width 1.7m, drainage trenching between ridge, enough wide 0.3m, deep 0.4m.Female parent is 2 years raw seedlings of Chinese catalpa (8611), and in late autumn or field planting in early spring, seeding row spacing is 1m × 1m, and namely every ridge two is arranged, spacing in the rows 1m.Bar is cut in liftoff 30cm place, in order to inspire more side shoot after field planting.
2) preparation in cuttage seedbed: the primary condition that place, cuttage seedbed should possess: draining, supply water good, stable firmly shade net can be set up；Seedbed is high with brick one-tenth 20cm, 100cm width, ground, length visual field and fixed substrate grooves, stays the management passage of 60cm between groove.After filling substrate, set up Small plastic shed again, mop floor with surrounding after cover film, can be as the criterion with brick compacting.
3) preparation of cutting medium: peat, perlite, Vermiculitum are stirred according to the volume ratio of 60:20:20, inserts in ready cuttage groove, and thickness 20cm waters permeable before cuttage, water permeable after guarantee more than hypothallus 15cm.
4) cultivation of fringe bar and gathering: late autumn arranges trench digging buriing farm manure or fertilizer, every 5kg between female parents two after fallen leaves, water before entering the winter permeable once.At the beginning of 3 months beginning of springs depending on soil moisture content water permeable once, at the beginning of 5 months leaves launch after buriing carbamide once, 100 grams every, after adopting fringe later every time mend execute once, water depending on soil moisture content.Late May adopts fringe to beginning first time at the beginning of 6 months, adopts fringe and starts to shelter from heat or light for first 10 days, it is ensured that intensity of illumination, less than 8000LX, inspires excessive growth new slightly.Shade net is packed up after adopting fringe, fertilising, water, after new slightly 20cm, enter next one circulation.Choose mounted blade when adopting fringe, branch that branch is delicate is cut, under the place's of cutting branch answer hands to pinch namely to break, the heart should be solid, green herein.
5) cuttage process: fringe bar is transferred to indoor immediately and cuts into cutting after gathering, axil is cut up and down, cutting stem segment length 10-15cm, 1-2 bud, retaining full leaf, a cutting slightly can be individually done on top, and base portion otch is immediately inserted into after dipping autogamy root-inducing powder in ready seedbed, cutting density is 10cm × 8cm, is advisable in the slightly unearthed face of the above pulvinus of the degree of depth.Water permeable after being plugged, build film, seal canopy moisturizing, enter follow-up schedule management link.
6) daily management after inserting: ensure the sealing of shed, thus ensureing the humidity in seedbed.Adopting spray pattern above shed, strong temperature maintains 28 DEG C-35 DEG C.Open thin film after 7 days, spray 1000 times of state's light brute force root-growing agents once, again build.After 20 days, rooting rate is up to more than 92%.
7) seedling exercising and transplanting: after cuttage 2 weeks, namely start the seedling exercising that leaks informaton, first 3 day daytime normal management, at 5 in afternoon opened shed two to next day 8, ventilation wet down.Within 3 days, for daytime, shed two being opened, ventilation wet down, at 5 in afternoon all opened shed to next day 8, ventilation wet down again.Remove thin film afterwards, water 1 ‰ carbamide rich water once.When cutting seedling second time lifts growth, root system is how plentiful, now can transplant.Transplanting Density is 50cm × 40cm.
Embodiment 2
The method adopting embodiment 1, in March, 2014 in July, 2015 launches field experiment in base, Nanjing Forestry University Baima, test material is Chinese catalpa 8611 and two kinds of common Chinese catalpa, every kind cutting that cuttage 1000 processed by embodiment 1 method respectively, and choose 1000 common hard stem cutting fringes respectively and without the twig cutting processed that shelters from heat or light as comparison, the experimental data of continuous 2 years shows: use the survival rate carrying out testing without the twig cutting processed that shelters from heat or light to be more than 20%, the survival rate that common hard stem cutting fringe carries out cuttage experiment is used to be only about 5%, but use the Chinese catalpa cutting survival rate that processed of this method respectively 92%, 90% and 85%, 87%, the Chinese catalpa cuttage survival rate being has increased significantly.
Claims (6)
1. the new method improving Chinese catalpa cottage propagation efficiency, it is characterised in that comprise the following steps:
1) foundation of cutting orchard: female parent is the raw seedling of 1-2, in late autumn or field planting in early spring, cuts bar, in order to inspire more side shoot, use sufficient fertilizer the daily management that carries out sheltering from heat or light in liftoff 30cm place after field planting；
2) preparation in cuttage seedbed: seedbed is high with brick one-tenth 20cm, 100cm width, ground, length visual field and fixed substrate grooves, stay the management passage of 60cm between groove；After filling substrate, set up Small plastic shed again, mop floor with surrounding after cover film, can be as the criterion with brick compacting；
3) preparation of cutting medium: peat, perlite, Vermiculitum are stirred according to the volume ratio of 60:20:20, inserts in ready cuttage groove, and thickness 20cm waters permeable before cuttage, water permeable after guarantee more than hypothallus 15cm；
4) cultivation of fringe bar with gather: fringe bar is cultured to late May at the beginning of 6 months, starts first time and adopts fringe, adopts fringe and start to shelter from heat or light for first 10 days, it is ensured that intensity of illumination, less than 8000LX, inspires excessive growth new slightly；Shade net is packed up after adopting fringe, fertilising, water, after new slightly 20cm, enter next one circulation；
5) cuttage: fringe bar is transferred to indoor immediately and cuts into cutting after gathering, base portion otch is immediately inserted into after dipping root-inducing powder in ready seedbed, waters permeable after being plugged, and builds film, seals canopy moisturizing, enters follow-up schedule management link；
6) daily management after inserting: temperature maintains 28 DEG C-35 DEG C, strengthens vegetable active, promotes early Fa Xingen；Opening thin film after 7 days, check seedbed moisture, cutting survives situation, sprays 1000 times of state's light brute force root-growing agents once, again builds；Within 8-10 days, starting to take root, when 20 days, rooting rate is the highest；
7) seedling exercising and transplanting: after cuttage 2 weeks, spot-check rooting rate, when reaching 50%, starts seedling exercising management of leaking informaton；When cutting seedling second time lifts growth, root system is how plentiful, now can transplant.
2. Flos Osmanthi Fragrantis nursery stock quick-growing cultivation method according to claim 1, it is characterised in that: in step 1), building and set up retractable movable shade, height 2.5m by 4m × 4m or 4m × 6m when cutting orchard is set up, shading rate requires to reach 90-95%.
3. Flos Osmanthi Fragrantis nursery stock quick-growing cultivation method according to claim 1, it is characterised in that: in step 4), late autumn arranges trench digging buriing farm manure or fertilizer, every 5kg between female parents two after fallen leaves, enter winter forward sight soil moisture content water permeable once；At the beginning of 3 months beginning of springs depending on soil moisture content water permeable once, at the beginning of 5 months leaves launch after buriing carbamide once, 100 grams every, after adopting fringe later every time mend execute once, water depending on soil moisture content.
4. Flos Osmanthi Fragrantis nursery stock quick-growing cultivation method according to claim 1, it is characterised in that: in step 4), choose mounted blade when adopting fringe, branch that branch is delicate is cut, under the place's of cutting branch answer hands to pinch namely to break, the heart should be solid, green herein.
5. Flos Osmanthi Fragrantis nursery stock quick-growing cultivation method according to claim 1, it is characterized in that: in step 5), cutting stem segment length 10-15cm, 1-2 bud, retaining full leaf, a cutting slightly can be individually done on top, and base portion otch is immediately inserted into after dipping root-inducing powder in ready seedbed, cutting density is 10cm × 8cm, is advisable in the slightly unearthed face of the above pulvinus of the degree of depth.
6. Flos Osmanthi Fragrantis nursery stock quick-growing cultivation method according to claim 1, it is characterised in that: in step 7), the seedling exercising that leaks informaton management particularly as follows: first 3 day daytime normal management, at 5 in afternoon opened shed two to next day 8, ventilation wet down；Within 3 days, for daytime, shed two being opened, ventilation wet down, at 5 in afternoon all opened shed to next day 8, ventilation wet down again；Remove thin film afterwards, water 1 ‰ carbamide rich water once.
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| CN106613961A (en) * | 2016-11-09 | 2017-05-10 | 湖北省林业科学研究院 | Cultivation method for micropropagation of catalpa bungei |
| CN107926315A (en) * | 2017-12-19 | 2018-04-20 | 安徽泓森高科林业股份有限公司 | Chinese catalpa bud cutting method for culturing seedlings |
| CN108076874A (en) * | 2017-12-26 | 2018-05-29 | 湖北省林业科学研究院 | A kind of Chinese catalpa softwood cutting propagation method |
| CN108713440A (en) * | 2018-05-29 | 2018-10-30 | 南京林业大学 | A kind of Chinese catalpa section stubble seedling breeding method |
| CN109005967A (en) * | 2018-07-18 | 2018-12-18 | 南京林业大学 | A kind of Chinese catalpa cottage method and Chinese catalpa seedling |
| CN109355236A (en) * | 2018-12-07 | 2019-02-19 | 山东土木启生物科技有限公司 | A kind of complex micro organism fungicide and preparation method with prevention and treatment nematode |
| CN110402749A (en) * | 2019-08-27 | 2019-11-05 | 中国林业科学研究院林业研究所 | A kind of construction method of ' Lip river Chinese catalpa No. 1 ' grafting cutting orchard |
| CN112470938A (en) * | 2021-01-14 | 2021-03-12 | 河南省农业科学院园艺研究所 | Micro-cutting seedling raising and rapid propagation method for catalpa bungei tissue culture seedlings |
| CN114902883A (en) * | 2022-05-16 | 2022-08-16 | 南京林业大学 | Method for intensively producing cutting shoots and twigs of catalpa bungei |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613961A (en) * | 2016-11-09 | 2017-05-10 | 湖北省林业科学研究院 | Cultivation method for micropropagation of catalpa bungei |
| CN106613961B (en) * | 2016-11-09 | 2018-11-02 | 湖北省林业科学研究院 | A kind of breeding method of Chinese catalpa micropropagation |
| CN107926315A (en) * | 2017-12-19 | 2018-04-20 | 安徽泓森高科林业股份有限公司 | Chinese catalpa bud cutting method for culturing seedlings |
| CN108076874A (en) * | 2017-12-26 | 2018-05-29 | 湖北省林业科学研究院 | A kind of Chinese catalpa softwood cutting propagation method |
| CN108713440A (en) * | 2018-05-29 | 2018-10-30 | 南京林业大学 | A kind of Chinese catalpa section stubble seedling breeding method |
| CN109005967A (en) * | 2018-07-18 | 2018-12-18 | 南京林业大学 | A kind of Chinese catalpa cottage method and Chinese catalpa seedling |
| CN109355236A (en) * | 2018-12-07 | 2019-02-19 | 山东土木启生物科技有限公司 | A kind of complex micro organism fungicide and preparation method with prevention and treatment nematode |
| CN110402749A (en) * | 2019-08-27 | 2019-11-05 | 中国林业科学研究院林业研究所 | A kind of construction method of ' Lip river Chinese catalpa No. 1 ' grafting cutting orchard |
| CN110402749B (en) * | 2019-08-27 | 2022-04-22 | 中国林业科学研究院林业研究所 | Construction method of grafting cutting orchard for' luohonia 1 |
| CN112470938A (en) * | 2021-01-14 | 2021-03-12 | 河南省农业科学院园艺研究所 | Micro-cutting seedling raising and rapid propagation method for catalpa bungei tissue culture seedlings |
| CN114902883A (en) * | 2022-05-16 | 2022-08-16 | 南京林业大学 | Method for intensively producing cutting shoots and twigs of catalpa bungei |
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Application publication date: 20160720 Assignee: Jiangsu Qinghao Landscape Horticulture Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: X2019320000325 Denomination of invention: Novel method for improving catalpa bungei cuttage propagation efficiency Granted publication date: 20190611 License type: Common License Record date: 20191203 Application publication date: 20160720 Assignee: Jurong Nengda family farm Co., Ltd Assignor: Nanjing Forestry University Contract record no.: X2019320000324 Denomination of invention: Novel method for improving catalpa bungei cuttage propagation efficiency Granted publication date: 20190611 License type: Common License Record date: 20191203 Application publication date: 20160720 Assignee: Meitao fruit professional cooperative, houbai Town, Jurong City Assignor: Nanjing Forestry University Contract record no.: X2019320000323 Denomination of invention: Novel method for improving catalpa bungei cuttage propagation efficiency Granted publication date: 20190611 License type: Common License Record date: 20191203 |
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