CN105754840A - Liquid culture medium subpackaging device - Google Patents
Liquid culture medium subpackaging device Download PDFInfo
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- CN105754840A CN105754840A CN201610252752.6A CN201610252752A CN105754840A CN 105754840 A CN105754840 A CN 105754840A CN 201610252752 A CN201610252752 A CN 201610252752A CN 105754840 A CN105754840 A CN 105754840A
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- liquid
- liquid cyclone
- culture plate
- tissue culture
- chute
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
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Abstract
Disclosed is a liquid culture medium subpackaging device.The liquid culture medium subpackaging device comprises a dispensette and is characterized by further comprising a connecting hose, a liquid cyclone and a support, wherein a liquid inlet of the liquid cyclone is communicated with a liquid outlet of the dispensette through the connecting hose, liquid split-flow outlets of the liquid cyclone face downwards and are identical with transversely-arranged or longitudinally-arranged cell culture holes of a cell culture plate in number, the liquid cyclone is supported on the support, the cell culture plate is disposed below the liquid cyclone, and the liquid cyclone or the cell culture plate can slide horizontally along a direction perpendicular to a connecting line of the liquid split-flow outlets of the liquid cyclone, so that different transversely-arranged or longitudinally-arranged cell culture holes of the cell culture plate can correspond to the liquid split-flow outlets of the liquid cyclone.The liquid culture medium subpackaging device is capable of not only subpackaging liquid culture medium into the cell culture plate efficiently and quickly but also reducing waste of the liquid culture medium and experiment cost.
Description
Technical field
The present invention relates to technical field of cell culture, particularly relate to fluid medium packaging device.
Background technology
Tissue Culture Plate, as the important tool in high flux culture technique, has more and more applied to biomedicine field.When to Tissue Culture Plate subpackage fluid medium, traditional mode is to be operated by bottleneck liquor separator or liquid-transfering gun, but bottleneck liquor separator, liquid-transfering gun are single channel dispensing apparatus, and subpackage efficiency is low, time length, microbiological contamination probability are big.Thus, multichannel liquid-transfering gun arises at the historic moment, it is capable of to Tissue Culture Plate quickly, subpackage fluid medium abreast, but multichannel liquid-transfering gun is on the one hand owing to its range is little, subpackage efficiency more still has much room for improvement, on the other hand, it must configure the loading slot adapted with multichannel rifle head, for guaranteeing that multichannel liquid-transfering gun can accurately, effectively imbitition culture medium, the fluid medium poured in loading slot is all above actual demand amount, this will cause the waste of fluid medium, and experimental cost is high.
Summary of the invention
For the problems referred to above, the invention provides fluid medium packaging device, its can not only efficiently, rapidly to Tissue Culture Plate subpackage fluid medium, and can effectively reduce the waste of fluid medium, reduce experimental cost.
Its technical scheme is such, and it includes bottleneck liquor separator, it is characterised in that: described packaging device also includes connecting tube, liquid cyclone and support；The liquid outlet of the liquid inlet of described liquid cyclone and described bottleneck liquor separator is by described connecting tube UNICOM, the separating liquid of described liquid cyclone goes out mouth down and arranges, its quantity is equal with the quantity of the line of Tissue Culture Plate or the cell culture well of file, described liquid cyclone is supported on described support, Tissue Culture Plate is positioned over below described liquid cyclone, described liquid cyclone or described Tissue Culture Plate can slide along being perpendicular to liquid cyclone each separating liquid outlet line direction level, make the different lines of Tissue Culture Plate or the cell culture well of file can export corresponding with the separating liquid of liquid cyclone.
Further, the chute being provided with through on described support, described chute is horizontally disposed with, it extends perpendicularly to each liquid outlet line of described liquid cyclone, described liquid cyclone or Tissue Culture Plate are supported on described chute and can along slide so that the different lines of Tissue Culture Plate or the cell culture well of file can be corresponding with the outlet of the separating liquid of liquid cyclone.
Further, described packaging device also includes the fixing seat of Tissue Culture Plate, the upper surface of the fixing seat of described Tissue Culture Plate is provided with the locating slot adapted with Tissue Culture Plate profile, the chute being provided with through on described support, described chute is horizontally disposed with, it extends perpendicularly to each liquid outlet line of described liquid cyclone, described liquid cyclone or the fixing seat supports of described Tissue Culture Plate are on described chute, and can along slide, make the different lines of the Tissue Culture Plate being positioned in its locating slot or the cell culture well of file can export corresponding with the separating liquid of liquid cyclone.
Further, some described chutes are vertically distributed on described support.
Further, described support includes base and supporter, and described supporter is fixed on described base, and described supporter is provided with described chute.
Preferably, described supporter includes a pair support beam, it is installed with support beam to described base lateral symmetry, described chute includes the first paired chute being symmetrically disposed on the medial surface that two support beams are relative, the two ends of described liquid cyclone are individually fixed on a pair support beam, the fixing seating of described Tissue Culture Plate is in supporting between beam a pair, being provided with flange to its lateral symmetry, and described flange is connected with described first slide.
It is further preferable that the second chute on described base upper surface, described second chute extends perpendicularly to each liquid outlet line of described liquid cyclone, and the bottom of the fixing seat of described Tissue Culture Plate is connected with described second slide.
More preferably, this is positioned on the position above described first chute is provided with draw-in groove symmetrically to supporting beam, described support beam be vertically distributed have several to draw-in groove, the position corresponding with draw-in groove, described liquid cyclone both sides is installed with fixture block, described fixture block is installed in described draw-in groove so that described liquid cyclone is fixed on described support beam.
Leg, described base or described support beam is had to be provided with level indicator it is further preferable that the corner of the lower surface of described base is threaded respectively.
Described bottleneck liquor separator can bear 121 DEG C, 20 minutes sterilization treatment, and without influence on moving the precision of liquid；Described liquid cyclone can bear 121 DEG C, 20 minutes sterilization treatment, and without influence on the precision of shunting.
After adopting the present invention, liquid cyclone is supported on support, Tissue Culture Plate is positioned over below liquid cyclone, liquid cyclone or Tissue Culture Plate can slide along being perpendicular to liquid cyclone each separating liquid outlet line direction level, make the different lines of Tissue Culture Plate or the cell culture well of file can export corresponding with the separating liquid of liquid cyclone, then pass through operation bottleneck liquor separator, fluid medium is made to draw out from wide mouthed bottle and be connected pipe, each separating liquid outlet of liquid cyclone is delivered in each cell culture well of this line or file, that can effectively reduce that cell culture well exports with separating liquid is directed at difficulty and reduces the arms swing amplitude of operator, it is thus possible to efficiently, rapidly to Tissue Culture Plate subpackage fluid medium；And owing to bottleneck liquor separator is directly connected with wide mouthed bottle, it is to avoid the use of loading slot such that it is able to effectively reduce the waste of fluid medium, reduce experimental cost.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention.
Fig. 2 is the structural representation in Fig. 1 after A place partial enlargement.
Detailed description of the invention
As shown in Figure 1 and Figure 2, fluid medium packaging device, it includes bottleneck liquor separator 3, connecting tube 5, liquid cyclone 2 and support 1；The liquid inlet 21 of liquid cyclone 2 and the liquid outlet 31 of bottleneck liquor separator 3 are by connecting tube 5 UNICOM, the separating liquid outlet 22 of liquid cyclone 2 is arranged down, its quantity is equal with the quantity of the line of Tissue Culture Plate or the cell culture well of file, liquid cyclone 2 is supported on support 1, Tissue Culture Plate is positioned over below liquid cyclone 2, Tissue Culture Plate can slide along being perpendicular to liquid cyclone each separating liquid outlet line direction level, make the different lines of Tissue Culture Plate or the cell culture well of file can export corresponding with the separating liquid of liquid cyclone.
Packaging device also includes the fixing seat 13 of Tissue Culture Plate, the upper surface of the fixing seat 13 of Tissue Culture Plate is provided with the locating slot 132 adapted with Tissue Culture Plate profile, the chute being provided with through on support 1, chute is horizontally disposed with, it extends perpendicularly to each liquid outlet line of liquid cyclone, in this embodiment, the fixing seat 13 of Tissue Culture Plate is supported on chute and can along slide so that the different lines of the Tissue Culture Plate being positioned in its locating slot or the cell culture well of file can be corresponding with the outlet of the separating liquid of liquid cyclone.Preferably, support includes base 11 and supporter, supporter includes a pair support beam 12, base 11 lateral symmetry it is installed with support beam 12, chute includes a pair first chutes 121 being symmetrically disposed on the medial surface that two support beams are relative, the two ends of liquid cyclone 2 are individually fixed on a pair support beam 12, and the fixing seat 13 of Tissue Culture Plate is placed between a pair support beam 12, is provided with to its lateral symmetry flange 131, and flange 131 and the first chute sliding 121 are dynamically connected.It is further preferable that the second chute 111 on base 11 upper surface, the second chute 111 extends perpendicularly to each liquid outlet line of liquid cyclone, and the bottom of the fixing seat 13 of Tissue Culture Plate is slidably connected with the second chute 111；This is positioned on the position above the first chute 121 is provided with draw-in groove 122 symmetrically to supporting beam 12, support beam 12 be vertically distributed have several to draw-in groove 122, the position corresponding with draw-in groove, liquid cyclone 2 both sides is installed with fixture block, fixture block is installed in draw-in groove 122 so that liquid cyclone is fixed on support beam；The corner of the lower surface of base 11 is threaded respectively leg 14, supports beam 12 and is provided with level indicator 15.
Support beam 12 be vertically distributed have several to draw-in groove 122, make liquid cyclone 2 can be fixed on differing heights position on support beam 12, to adapt to the Tissue Culture Plate of differing heights, in other embodiments, some the first chutes being vertically distributed can be set on support 1, fix the relative position of seat to adapt to liquid cyclone by changing Tissue Culture Plate, also can reach same effect；Second chute 111 and the first chute 121 cooperate so that the fixing seat of Tissue Culture Plate slides steadily and can prevent it from offseting.
In accompanying drawing 1,4 is the wide mouthed bottle equipped with fluid medium；Wherein, liquid cyclone 2 and bottleneck liquor separator 3 are prior art, its principle is comparatively known for a person skilled in the art, repeats no more.
The operation principle of the present invention is as follows: 1, calculate the interpolation total amount of fluid medium needed for Tissue Culture Plate line or file cell culture well；2, the output knob of bottleneck liquor separator 3 is regulated so that it is the fluid medium of output is equal with adding total amount every time；3, Tissue Culture Plate is promoted gently so that Tissue Culture Plate needs each line of adding liquid culture medium or the cell culture well of file corresponding with the outlet of liquid cyclone each separating liquid；4, starting bottleneck liquor separator, first get rid of the air in connecting tube and liquid cyclone, the waste liquid produced in this process can enter in the locating slot on the fixing seat of Tissue Culture Plate；5, continuing to start bottleneck liquor separator, fluid medium is connected after pipe, liquid cyclone successively from wide mouthed bottle and flows in each cell culture well；6, promote Tissue Culture Plate, continue the cell culture well adding liquid culture medium to next line or file.
Claims (10)
1. fluid medium packaging device, it includes bottleneck liquor separator, it is characterised in that: described packaging device also includes connecting tube, liquid cyclone and support；The liquid outlet of the liquid inlet of described liquid cyclone and described bottleneck liquor separator is by described connecting tube UNICOM, the separating liquid of described liquid cyclone goes out mouth down and arranges, its quantity is equal with the quantity of the line of Tissue Culture Plate or the cell culture well of file, described liquid cyclone is supported on described support, Tissue Culture Plate is positioned over below described liquid cyclone, described liquid cyclone or described Tissue Culture Plate can slide along being perpendicular to liquid cyclone each separating liquid outlet line direction level, make the different lines of Tissue Culture Plate or the cell culture well of file can export corresponding with the separating liquid of liquid cyclone.
2. fluid medium packaging device according to claim 1, it is characterized in that: the chute being provided with through on described support, described chute is horizontally disposed with, it extends perpendicularly to each liquid outlet line of described liquid cyclone, described liquid cyclone or Tissue Culture Plate are supported on described chute and can along slide so that the different lines of Tissue Culture Plate or the cell culture well of file can be corresponding with the outlet of the separating liquid of liquid cyclone.
3. fluid medium packaging device according to claim 1, it is characterized in that: described packaging device also includes the fixing seat of Tissue Culture Plate, the upper surface of the fixing seat of described Tissue Culture Plate is provided with the locating slot adapted with Tissue Culture Plate profile, the chute being provided with through on described support, described chute is horizontally disposed with, it extends perpendicularly to each liquid outlet line of described liquid cyclone, described liquid cyclone or the fixing seat supports of described Tissue Culture Plate are on described chute, and can along slide, make the different lines of the Tissue Culture Plate being positioned in its locating slot or the cell culture well of file can export corresponding with the separating liquid of liquid cyclone.
4. the fluid medium packaging device according to Claims 2 or 3, it is characterised in that: some described chutes are vertically distributed on described support.
5. fluid medium packaging device according to claim 3, it is characterised in that: described support includes base and supporter, and described supporter is fixed on described base, and described supporter is provided with described chute.
6. fluid medium packaging device according to claim 5, it is characterized in that: described supporter includes a pair support beam, it is installed with support beam to described base lateral symmetry, described chute includes the first paired chute being symmetrically disposed on the medial surface that two support beams are relative, the two ends of described liquid cyclone are individually fixed on a pair support beam, the fixing seating of described Tissue Culture Plate is in supporting between beam a pair, being provided with flange to its lateral symmetry, and described flange is connected with described first slide.
7. fluid medium packaging device according to claim 6, it is characterized in that: the second chute on described base upper surface, described second chute extends perpendicularly to each liquid outlet line of described liquid cyclone, and the bottom of the fixing seat of described Tissue Culture Plate is connected with described second slide.
8. fluid medium packaging device according to claim 6, it is characterized in that: this is positioned on the position above described first chute is provided with draw-in groove symmetrically to supporting beam, described support beam be vertically distributed have several to draw-in groove, the position corresponding with draw-in groove, described liquid cyclone both sides is installed with fixture block, described fixture block is installed in described draw-in groove so that described liquid cyclone is fixed on described support beam.
9. according to described fluid medium packaging device arbitrary in claim 5～8, it is characterised in that: the corner of the lower surface of described base is threaded respectively has leg, described base or described support beam to be provided with level indicator.
10. according to claims 1 to 3, arbitrary described fluid medium packaging device in 5～8, it is characterised in that: described bottleneck liquor separator can bear 121 DEG C, 20 minutes sterilization treatment, and without influence on moving the precision of liquid；Described liquid cyclone can bear 121 DEG C, 20 minutes sterilization treatment, and without influence on the precision of shunting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610252752.6A CN105754840B (en) | 2016-04-22 | 2016-04-22 | Fluid nutrient medium packaging device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610252752.6A CN105754840B (en) | 2016-04-22 | 2016-04-22 | Fluid nutrient medium packaging device |
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| Publication Number | Publication Date |
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| CN105754840A true CN105754840A (en) | 2016-07-13 |
| CN105754840B CN105754840B (en) | 2018-06-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610252752.6A Active CN105754840B (en) | 2016-04-22 | 2016-04-22 | Fluid nutrient medium packaging device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109197589A (en) * | 2018-10-10 | 2019-01-15 | 江西省科学院生物资源研究所 | A kind of solid medium preparation method of improvement |
| CN113697146A (en) * | 2021-08-20 | 2021-11-26 | 苏州博腾生物制药有限公司 | Cell subpackaging method in cell bank building process |
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Patent Citations (6)
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| Publication number | Publication date |
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| CN105754840B (en) | 2018-06-26 |
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Effective date of registration: 20210824 Address after: 610000 South Sixth Road 669, Chengdu Economic and Technological Development Zone, Sichuan Province Patentee after: CHENGDU YINGDE BIO-MEDICAL EQUIPMENT TECHNOLOGY Co.,Ltd. Patentee after: Jiangnan University Address before: 213000 d3010, No.26 Huashan Middle Road, Xinbei District, Changzhou City, Jiangsu Province Patentee before: CHANGZHOU YINGDE BIOTECHNOLOGY Co.,Ltd. Patentee before: Jiangnan University |