A kind of fluid medium packaging device
Technical field
This utility model relates to technical field of cell culture, particularly relates to a kind of fluid medium packaging device.
Background technology
Tissue Culture Plate, as the important tool in high flux culture technique, the most more and more applies to biomedicine field.When to Tissue Culture Plate subpackage fluid medium, traditional mode is to be operated by bottleneck liquor separator or liquid-transfering gun, but bottleneck liquor separator, liquid-transfering gun are single channel dispensing apparatus, and subpackage efficiency is low, time length, microbiological contamination probability are big.Thus, multichannel liquid-transfering gun arises at the historic moment, it is capable of to Tissue Culture Plate quickly, subpackage fluid medium abreast, but multichannel liquid-transfering gun is on the one hand little due to its range, subpackage efficiency more still has much room for improvement, on the other hand, it must configure the loading slot adapted with multichannel rifle head, for guaranteeing that multichannel liquid-transfering gun can accurately, effectively draw fluid medium, the fluid medium poured in loading slot is all above actual demand amount, this will cause the waste of fluid medium, and experimental cost is high.
Utility model content
For the problems referred to above, this utility model provides a kind of fluid medium packaging device, its can not only efficiently, rapidly to Tissue Culture Plate subpackage fluid medium, and can effectively reduce the waste of fluid medium, reduce experimental cost.
Its technical scheme is such, and it includes bottleneck liquor separator, it is characterised in that: described packaging device also includes connecting tube, liquid cyclone and support;The liquid outlet of the liquid inlet of described liquid cyclone and described bottleneck liquor separator is by described connecting tube UNICOM, the separating liquid of described liquid cyclone goes out mouth down and arranges, its quantity is equal with the quantity of the line of Tissue Culture Plate or the cell culture well of file, described liquid cyclone is supported on described support, Tissue Culture Plate is positioned over below described liquid cyclone, described liquid cyclone or described Tissue Culture Plate can slide along being perpendicular to liquid cyclone each separating liquid outlet line direction level, make the different lines of Tissue Culture Plate or the cell culture well of file can export corresponding with the separating liquid of liquid cyclone.
Further, described support is provided with the chute run through, described chute is horizontally disposed with, it extends perpendicularly to each liquid outlet line of described liquid cyclone, described liquid cyclone or Tissue Culture Plate are supported on described chute and can be along slide so that the different lines of Tissue Culture Plate or the cell culture well of file can be corresponding with the outlet of the separating liquid of liquid cyclone.
Further, described packaging device also includes that Tissue Culture Plate fixes seat, described Tissue Culture Plate is fixed the upper surface of seat and is provided with the locating slot adapted with Tissue Culture Plate profile, described support is provided with the chute run through, described chute is horizontally disposed with, it extends perpendicularly to each liquid outlet line of described liquid cyclone, described liquid cyclone or described Tissue Culture Plate fix seat supports on described chute, and can be along slide, make the different lines of the Tissue Culture Plate being positioned in its locating slot or the cell culture well of file can export corresponding with the separating liquid of liquid cyclone.
Further, some described chutes are vertically distributed on described support.
Further, described support includes that base and supporter, described supporter are fixed on described base, and described supporter is provided with described chute.
Preferably, described supporter includes a pair support beam, it is installed with support beam to described base lateral symmetry, described chute includes the first paired chute being symmetrically disposed on the medial surface that two support beams are relative, the two ends of described liquid cyclone are individually fixed in a pair support beam, described Tissue Culture Plate fixes seating between a pair support beam, be provided with flange to its lateral symmetry, and described flange is connected with described first slide.
It is further preferable that the second chute on described base upper surface, described second chute extends perpendicularly to each liquid outlet line of described liquid cyclone, and described Tissue Culture Plate is fixed the bottom of seat and is connected with described second slide.
More preferably, support beam is positioned on the position above described first chute and is provided with draw-in groove symmetrically by this, described support beam is vertically distributed several to draw-in groove, the position corresponding with draw-in groove, described liquid cyclone both sides is installed with fixture block, described fixture block is installed in described draw-in groove so that described liquid cyclone is fixed in described support beam.
Leg, described base or described support beam is had to be provided with level indicator it is further preferable that the corner of the lower surface of described base is the most threaded.
Described bottleneck liquor separator can bear 121 DEG C, 20 minutes sterilization treatment, and does not interferes with the precision moving liquid;Described liquid cyclone can bear 121 DEG C, 20 minutes sterilization treatment, and does not interferes with the precision of shunting.
After using this utility model, liquid cyclone is supported on support, Tissue Culture Plate is positioned over below liquid cyclone, liquid cyclone or Tissue Culture Plate can slide along being perpendicular to liquid cyclone each separating liquid outlet line direction level, make the different lines of Tissue Culture Plate or the cell culture well of file can export corresponding with the separating liquid of liquid cyclone, then by operation bottleneck liquor separator, fluid medium is made to draw out and through connecting tube from wide mouthed bottle, each separating liquid outlet of liquid cyclone is delivered in each cell culture well of this line or file, that can effectively reduce that cell culture well exports with separating liquid is directed at difficulty and reduces the arms swing amplitude of operator, it is thus possible to efficiently, rapidly to Tissue Culture Plate subpackage fluid medium;And owing to bottleneck liquor separator is directly connected with wide mouthed bottle, it is to avoid the use of loading slot such that it is able to effectively reduce the waste of fluid medium, reduce experimental cost.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is structural representation after partial enlargement at A in Fig. 1.
Detailed description of the invention
As shown in Figure 1 and Figure 2, a kind of fluid medium packaging device, it includes bottleneck liquor separator 3, connecting tube 5, liquid cyclone 2 and support 1;The liquid inlet 21 of liquid cyclone 2 and the liquid outlet 31 of bottleneck liquor separator 3 are by connecting tube 5 UNICOM, the separating liquid outlet 22 of liquid cyclone 2 is arranged down, its quantity is equal with the quantity of the line of Tissue Culture Plate or the cell culture well of file, liquid cyclone 2 is supported on support 1, Tissue Culture Plate is positioned over below liquid cyclone 2, Tissue Culture Plate can slide along being perpendicular to liquid cyclone each separating liquid outlet line direction level, make the different lines of Tissue Culture Plate or the cell culture well of file can export corresponding with the separating liquid of liquid cyclone.
Packaging device also includes that Tissue Culture Plate fixes seat 13, Tissue Culture Plate is fixed the upper surface of seat 13 and is provided with the locating slot 132 adapted with Tissue Culture Plate profile, support 1 is provided with the chute run through, chute is horizontally disposed with, it extends perpendicularly to each liquid outlet line of liquid cyclone, in this embodiment, Tissue Culture Plate is fixed seat 13 and is supported on chute and can be along slide so that the different lines of the Tissue Culture Plate being positioned in its locating slot or the cell culture well of file can be corresponding with the outlet of the separating liquid of liquid cyclone.Preferably, support includes base 11 and supporter, supporter includes a pair support beam 12, it is installed with support beam 12 to base 11 lateral symmetry, chute includes a pair first chutes 121 being symmetrically disposed on the medial surface that two support beams are relative, the two ends of liquid cyclone 2 are individually fixed in a pair support beam 12, and Tissue Culture Plate is fixed seat 13 and is placed between a pair support beam 12, is provided with to its lateral symmetry flange 131, and flange 131 is dynamically connected with the first chute sliding 121.It is further preferable that the second chute 111 on base 11 upper surface, the second chute 111 extends perpendicularly to each liquid outlet line of liquid cyclone, and Tissue Culture Plate is fixed the bottom of seat 13 and is slidably connected with the second chute 111;Support beam 12 is positioned on the position above the first chute 121 and is provided with draw-in groove 122 symmetrically by this, support beam 12 is vertically distributed several to draw-in groove 122, the position corresponding with draw-in groove, liquid cyclone 2 both sides is installed with fixture block, fixture block is installed in draw-in groove 122 so that liquid cyclone is fixed in support beam;The corner of the lower surface of base 11 is the most threaded leg 14, and support beam 12 is provided with level indicator 15.
Support beam 12 is vertically distributed several to draw-in groove 122, make liquid cyclone 2 can be fixed on differing heights position in support beam 12, to adapt to the Tissue Culture Plate of differing heights, in other embodiments, some the first chutes being vertically distributed can be set on support 1, fix the relative position of seat to adapt to liquid cyclone by changing Tissue Culture Plate, also can reach same effect;Second chute 111 cooperates with the first chute 121 so that Tissue Culture Plate is fixed seat and slided steadily and can prevent it from offseting.
In accompanying drawing 1,4 is the wide mouthed bottle equipped with fluid medium;Wherein, liquid cyclone 2 and bottleneck liquor separator 3 are prior art, the most more know its principle, repeat no more.
Operation principle of the present utility model is as follows: 1, calculate the interpolation total amount of fluid medium needed for Tissue Culture Plate line or file cell culture well;2, the output knob of bottleneck liquor separator 3 is regulated so that it is the fluid medium of output is equal with adding total amount every time;3, Tissue Culture Plate is promoted gently so that on Tissue Culture Plate, need each line of adding liquid culture medium or the cell culture well separating liquid each with liquid cyclone of file to export corresponding;4, starting bottleneck liquor separator, first get rid of the air in connecting tube and liquid cyclone, the waste liquid produced during being somebody's turn to do can enter in the locating slot that Tissue Culture Plate is fixed on seat;5, continuing to start bottleneck liquor separator, fluid medium flows in each cell culture well successively from wide mouthed bottle after connecting tube, liquid cyclone;6, promote Tissue Culture Plate, continue the cell culture well adding liquid culture medium to next line or file.