CN105670919A - Cover-pressing type disposable sperm counting chamber - Google Patents
Cover-pressing type disposable sperm counting chamber Download PDFInfo
- Publication number
- CN105670919A CN105670919A CN201610005803.5A CN201610005803A CN105670919A CN 105670919 A CN105670919 A CN 105670919A CN 201610005803 A CN201610005803 A CN 201610005803A CN 105670919 A CN105670919 A CN 105670919A
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- China
- Prior art keywords
- coverslip
- sperm
- support post
- sample platform
- pressure type
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Sampling And Sample Adjustment (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Microscoopes, Condenser (AREA)
- Analytical Chemistry (AREA)
Abstract
The invention provides a cover-pressing type disposable sperm counting chamber including a glass slide, a coverslip, supporting stand columns and a sample adding stage; the glass slide is provided with the supporting stand columns and the sample adding stage; when the coverslip is arranged on the glass slide, the lower side face of the coverslip and the upper end faces of the supporting stand columns are laminated; a sperm storage space is formed between the sample adding stage and the coverslip. A plurality of the supporting stand columns are arranged and are respectively distributed in the circumferential direction of the sample adding stage; the sample adding stage is arranged among the plurality of the supporting stand columns. A sample is firstly added, and then the coverslip is covered, so that a special viscous sperm sample is convenient to add, the sample adding speed and uniformity of the special specimen are improved, and subsequent performing of sperm image analysis is facilitated.
Description
Technical field
The present invention relates to cytology's count tool that field of medical examination laboratory uses, in particular it relates to a kind of disposable sperm count pond of lid pressure type.
Background technology
Sperm count pond is the use of the sample process before being used to human sperm quality analysis. Existing MACRO ruby (seminal fluid) sperm count pond is widely used in routine examination and the metering test of the humoral specimens such as seminal fluid, prostate, cervical mucus. Sperm count pond is used primarily on all kinds of biological microscope containing video camera and microscopic imaging device, this kind of instrument bag is containing light source, object stage, composition lens, camera interface and video camera, sperm in counting chamber images on video camera through micro-amplification, shooting function by the sperm Projection Display in imaging region in counting chamber out, carries out sperm image procossing for follow-up.
Through the retrieval to prior art, it has been found that application number is 2011202208595, and name is called a kind of disposable cell counting count board of the disclosure of the invention of a kind of disposable cell counting count board, its microscope slide is stained with two groups of support drop groups side by side; Having 4 support drops spaced apart in each group of support drop group, every supports the siliceous glass pearl containing multiple control counting chamber degree of depth in drop. During use, take microscope slide one, specimen is added on one group and supports between drop group, covered also presses lightly on downwards coverslip to extreme lower position with finger abdominal part, namely counting chamber it is assembled into, can support the use with biological microscope, carry out cell counting for Clinical Laboratory mechanism or other experimenters and specimen is observed, be particularly well-suited to the grain count that density is relatively low. Such as can be used for Mucus interaction in vitro tests and semen white blood cell detection (Goodpasture's staining) etc. But in the invention, observed object, not in same focal plane, need to be focused and just can see, sperm motility, not a focal plane, can cause spermatozoon track to lose, be unfavorable for motility of sperm classification.
Summary of the invention
For defect of the prior art, it is an object of the invention to provide a kind of disposable sperm count pond of lid pressure type.
According to the disposable sperm count pond of lid pressure type provided by the invention, including microscope slide, coverslip, support post and application of sample platform;
Described microscope slide is provided with described support post and described application of sample platform;
When described coverslip is arranged on described microscope slide, fit in the upper surface of the downside of described coverslip and described support post, forms sperm parking space between described application of sample platform and described coverslip.
Preferably, the quantity of described support post is multiple;
Multiple described support posts are distinguished along the circumference of described application of sample platform; Described application of sample platform is arranged between multiple described support post.
Preferably, the quantity of described support post is 3.
Preferably, described microscope slide adopts lucite to make;
Described coverslip adopts optical glass to make.
Preferably, the diameter of described application of sample platform is 6mm;
Described sperm parking space is of a size of, and diameter is 6mm, and the degree of depth is 10um, 20um, 100um, and wherein, the degree of depth is that the sperm parking space of 10um is for conventional sense; The degree of depth is that the sperm parking space of 20um is for few essence specimen; The degree of depth is that the sperm parking space of 100um is for azoospermia Samples detection.
Preferably, described microscope slide is provided with holding tank; The size of described holding tank and described coverslip matches;
The bottom land of described holding tank states support post and described application of sample platform described in being provided with.
Preferably, the two ends of described holding tank opening's edge are provided with the first fin and the second fin;
Wherein, described first fin and described second fin are L-shaped; Described holding tank is rectangular.
Compared with prior art, the present invention has following beneficial effect:
1, elder generation of the present invention application of sample coverslip again, it is simple to special dense thick sperm sample application, improves the feed rate of special specimen, uniformity, facilitates and follow-up carries out sperm graphical analysis;
2, the counting chamber sperm parking space in the present invention is of a size of diameter is 6mm, the degree of depth is that 10um can form monolayer, independent spermatid, do not overlap, the phenomenons such as accumulation, standard pond accurately guarantees that sperm is in together in focal length aspect deeply, more can guarantee that to sperm quantity, density, vigor Accurate Analysis;
3, the present invention does not need the medical disposable material of Cleaning count plate, alleviates working strength, operation cleaning simplicity, it is possible to prevent cross-contamination.
Accompanying drawing explanation
By reading detailed description non-limiting example made with reference to the following drawings, the other features, objects and advantages of the present invention will become more apparent upon:
Fig. 1 is a kind of structural representation of the present invention;
Fig. 2 is the another kind of structural representation of the present invention.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail. Following example will assist in those skilled in the art and are further appreciated by the present invention, but do not limit the present invention in any form. It should be pointed out that, to those skilled in the art, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement. These broadly fall into protection scope of the present invention.
At the present embodiment, the disposable sperm count pond of lid pressure type provided by the invention, including microscope slide, coverslip, support post and application of sample platform;
Described microscope slide is provided with described support post and described application of sample platform;
When described coverslip is arranged on described microscope slide, fit in the upper surface of the downside of described coverslip and described support post, forms sperm parking space between described application of sample platform and described coverslip.
The quantity of described support post is multiple; Multiple described support posts are distinguished along the circumference of described application of sample platform; Described application of sample platform is arranged between multiple described support post.
The quantity of described support post is 3.
Described microscope slide adopts lucite to make; Described coverslip adopts optical glass to make.
The diameter of described application of sample platform is 6mm; Described sperm parking space is of a size of, and diameter is 6mm, and the degree of depth is 10um, 20um, 100um, and wherein, the degree of depth is that the sperm parking space of 10um is for conventional sense;The degree of depth is that the sperm parking space of 20um is for few essence specimen; The degree of depth is that the sperm parking space of 100um is for azoospermia Samples detection.
Described microscope slide is provided with holding tank; The size of described holding tank and described coverslip matches;
The bottom land of described holding tank states support post and described application of sample platform described in being provided with.
The two ends of described holding tank opening's edge are provided with the first fin and the second fin;
Wherein, described first fin and described second fin are L-shaped; Described holding tank is rectangular.
Requirement according to " WHO human seminal fluid checks and treatment of laboratory handbook (the 5th edition) ", by as follows with the contrast of MACRO ruby (seminal fluid) sperm count pond for the present invention:
It follows that use invention to carry out the analysis pre-treatment of human sperm cell sample, working strength can be alleviated, it is prevented that cross-contamination.
In the present embodiment, elder generation of the present invention application of sample coverslip again, it is simple to special dense thick sperm sample application, improves the feed rate of special specimen, uniformity, facilitates and follow-up carries out sperm graphical analysis; It is 6mm that counting chamber sperm parking space in the present invention is of a size of diameter, the degree of depth is that 10um can form monolayer, independent spermatid, do not overlap, the phenomenons such as accumulation, standard pond accurately guarantees that sperm is in together in focal length aspect deeply, more can guarantee that to sperm quantity, density, vigor Accurate Analysis; The present invention does not need the medical disposable material of Cleaning count plate, alleviates working strength, operation cleaning simplicity, it is possible to prevent cross-contamination.
Above specific embodiments of the invention are described. It is to be appreciated that the invention is not limited in above-mentioned particular implementation, those skilled in the art can make various deformation or amendment within the scope of the claims, and this has no effect on the flesh and blood of the present invention.
Claims (7)
1. the disposable sperm count pond of lid pressure type, it is characterised in that include microscope slide, coverslip, support post and application of sample platform;
Described microscope slide is provided with described support post and described application of sample platform;
When described coverslip is arranged on described microscope slide, fit in the upper surface of the downside of described coverslip and described support post, forms sperm parking space between described application of sample platform and described coverslip.
2. the disposable sperm count pond of lid pressure type according to claim 1, it is characterised in that the quantity of described support post is multiple;
Multiple described support posts are distinguished along the circumference of described application of sample platform; Described application of sample platform is arranged between multiple described support post.
3. the disposable sperm count pond of lid pressure type according to claim 2, it is characterised in that the quantity of described support post is 3.
4. the disposable sperm count pond of lid pressure type according to claim 1, it is characterised in that described microscope slide adopts lucite to make;
Described coverslip adopts optical glass to make.
5. the disposable sperm count pond of lid pressure type according to claim 1, it is characterised in that the diameter of described application of sample platform is 6mm;
Described sperm parking space is of a size of, and diameter is 6mm, and the degree of depth is 10um, 20um, 100um.
6. the disposable sperm count pond of lid pressure type according to claim 1, it is characterised in that described microscope slide is provided with holding tank; The size of described holding tank and described coverslip matches;
The bottom land of described holding tank states support post and described application of sample platform described in being provided with.
7. the disposable sperm count pond of lid pressure type according to claim 6, it is characterised in that the two ends of described holding tank opening's edge are provided with the first fin and the second fin;
Wherein, described first fin and described second fin are L-shaped;Described holding tank is rectangular.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610005803.5A CN105670919A (en) | 2016-01-05 | 2016-01-05 | Cover-pressing type disposable sperm counting chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610005803.5A CN105670919A (en) | 2016-01-05 | 2016-01-05 | Cover-pressing type disposable sperm counting chamber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105670919A true CN105670919A (en) | 2016-06-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610005803.5A Pending CN105670919A (en) | 2016-01-05 | 2016-01-05 | Cover-pressing type disposable sperm counting chamber |
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| CN (1) | CN105670919A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769804A (en) * | 2017-02-26 | 2017-05-31 | 济南继德生物科技有限公司 | Disposable multifunction cassette counting chamber |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2297731Y (en) * | 1997-05-28 | 1998-11-18 | 徐元诚 | Counting plate for sperm |
| CN101477024A (en) * | 2008-12-31 | 2009-07-08 | 刘瑜 | Visible component counting pool, its production method and use method |
| CN202075277U (en) * | 2011-05-10 | 2011-12-14 | 北京华方神火科技有限公司 | Sperm detecting room |
| CN202093554U (en) * | 2011-06-27 | 2011-12-28 | 深圳市博锐德生物科技有限公司 | Disposable cell counting plate |
| CN202710459U (en) * | 2012-08-10 | 2013-01-30 | 徐元诚 | Double-pool sperm counting plate |
| CN204848871U (en) * | 2015-06-09 | 2015-12-09 | 上海北昂精密光电仪器股份有限公司 | Sperm count pond |
-
2016
- 2016-01-05 CN CN201610005803.5A patent/CN105670919A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2297731Y (en) * | 1997-05-28 | 1998-11-18 | 徐元诚 | Counting plate for sperm |
| CN101477024A (en) * | 2008-12-31 | 2009-07-08 | 刘瑜 | Visible component counting pool, its production method and use method |
| CN202075277U (en) * | 2011-05-10 | 2011-12-14 | 北京华方神火科技有限公司 | Sperm detecting room |
| CN202093554U (en) * | 2011-06-27 | 2011-12-28 | 深圳市博锐德生物科技有限公司 | Disposable cell counting plate |
| CN202710459U (en) * | 2012-08-10 | 2013-01-30 | 徐元诚 | Double-pool sperm counting plate |
| CN204848871U (en) * | 2015-06-09 | 2015-12-09 | 上海北昂精密光电仪器股份有限公司 | Sperm count pond |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769804A (en) * | 2017-02-26 | 2017-05-31 | 济南继德生物科技有限公司 | Disposable multifunction cassette counting chamber |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 200090 Shanghai city Yangpu District Zhoujiazui Road No. 3805 room 4017 Applicant after: Shanghai north medicine Polytron Technologies Inc Address before: 200071 Shanghai city Yangpu District Zhoujiazui Road No. 3805 room 4017 Applicant before: The high accurate photoelectric device limited company in north, Shanghai |
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160615 |