CN105660867B - A kind of glutathione Yoghourt peptide piece and preparation method thereof - Google Patents

A kind of glutathione Yoghourt peptide piece and preparation method thereof Download PDF

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Publication number
CN105660867B
CN105660867B CN201610090504.6A CN201610090504A CN105660867B CN 105660867 B CN105660867 B CN 105660867B CN 201610090504 A CN201610090504 A CN 201610090504A CN 105660867 B CN105660867 B CN 105660867B
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peptide
glutathione
fermentation
yoghourt
milk
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CN105660867A (en
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叶春江
刘月辉
王元秀
李慧杰
王凯
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University of Jinan
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University of Jinan
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/18Milk in dried and compressed or semi-solid form
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1307Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1322Inorganic compounds; Minerals, including organic salts thereof, oligo-elements; Amino-acids, peptides, protein-hydrolysates or derivatives; Nucleic acids or derivatives; Yeast extract or autolysate; Vitamins; Antibiotics; Bacteriocins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A kind of glutathione Yoghourt peptide piece, it is prepared by following raw materials: milk 300~500, soybean peptide 5~10, corn peptide 5~10, maltodextrin 5~10, white granulated sugar 5 ~ 8, glutathione 2~10, vitamin C 4~15, cysteine 0.1~1, lipoic acid 0.2~2, simultaneously, provide preparation method, Yoghourt peptide piece prepared by the present invention not only nutrition more comprehensively, while also have it is anti-oxidant, removing toxic substances and deferring senility.

Description

A kind of glutathione Yoghourt peptide piece and preparation method thereof
Technical field
The present invention relates to functional food manufacture fields, and in particular to a kind of glutathione Yoghourt peptide piece and preparation method thereof.
Background technique
With the quickening of social rhythm and the increase of people's operating pressure, many pathogenetic trend of disease are more and more younger Change, this variation is related to eating habit with the long-term life of people to a certain extent.Such as it is long-term stay up late, excessively fatigue and Dietary structure is single, excessive fast food diet etc..Therefore, how fast, easily to supplement it is balanced nutritious, and with normal diet Or the active factors of snack food regularity intake tool healthcare function are the projects for being worth further investigation and exploitation.
The tissue of 80% or more human body is to be made of protein, therefore proteins and peptides are nutrition important in human body Element can supplement rapidly needed by human body nutrition and regain one's strength.Related product such as soy-bean peptide, corn peptides products and pure Milk, flavor cream, Yoghourt (piece) etc., are all the nutrient protein replenishers being widely appreciated by consumers.Simultaneously also containing weight in polypeptide Want the active peptide of bioactivity.Such as high F value oligopeptide, corn peptide, antibacterial peptide, glutathione, the peptide that relieves the effect of alcohol have blood pressure lowering, drop blood It rouge, liver protection and relieves the effect of alcohol and other effects.Human body can absorb the nutritional ingredients such as amino acid in the form of polypeptide simultaneously, thus polypeptide is ratio The faster nutritional ingredient of protein adsorption, to reach the health purpose for relieving fatigue, supplementing the nutrients.Currently, being with dairy products The function intensified novel dairy product of carrier exploitation nutrition and health care has become the important developing direction in one, dairy products field.
But lack a kind of Yoghourt peptide flake products for combining glutathione and Yoghourt currently on the market, it is vast to facilitate Consumer mends the approach of food glutathione, meanwhile, effectively improve the taste and healthcare function of fermented dairy product.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of glutathione Yoghourt peptide pieces, meanwhile, provide its system Preparation Method.Yoghourt peptide piece provided by the invention not only nutrition more comprehensively, while also have it is anti-oxidant, removing toxic substances and anti-aging function Energy.
Technical scheme is as follows:
A kind of glutathione Yoghourt peptide piece, is prepared by following raw materials: milk 300~500, soybean peptide 5~10, corn Peptide 5~10, maltodextrin 5~10, white granulated sugar 5 ~ 8, glutathione 2~10, vitamin C 4~15, cysteine 0.1~1, Lipoic acid 0.2~2.
Sweetener flavoring can also be added in the present invention, and sweetener is fructose syrup, antierythrite, xylitol, honey, white sand One of sugar etc. are a variety of.
The dry matter content of the milk is 11%.
The preparation method of the glutathione Yoghourt peptide piece, includes the following steps:
(1) basestocks mix: soybean peptide, corn peptide, maltodextrin, white granulated sugar are added to milk (dry matter content 11%) it, stirs, boils 10 minutes, with sterilization, be cooled to room temperature;
(2) yeast aerobic fermentation: to 1) in system in inoculation 5% (OD600=300) Saccharomyces cerevisiae or saccharomyces cerevisiae Brewer's wort culture, aerobic fermentation 5~8 hours in shaking table or reactor, temperature are 28~30 DEG C;
(3) lactic acid bacteria anaerobic fermentation: aseptically to 2) in culture in inoculation 10% lactic acid bacteria agent, temperature 42~45 DEG C of degree, anaerobic fermentation 8~12 hours, condenses system;Fermentation is that 4.0 or so terminations are fermented to pH;Adding citric acid makes Its pH is 3.8;
(4) after-ripening: by 3) obtained in fermentation material in 4~8 DEG C it is cold set 8~12 hours, obtain flavor substance therein Adequately accumulation;
(5) be concentrated: by 4) in fermentation material be concentrated by evaporation to its Solid content and reach for 60~80 DEG C under vacuum or reduced pressure 35~50%;
(6) it adds anti-oxidant combination: adding glutathione, VC, cysteine, lipoic acid in Xiang Shangshu concentrate, with stirring Mix machine mixing;
(7) homogeneous: by 6) in the material that mixes carry out homogeneous, 30~40MPa of pressure keeps material more uniform and thin It is sliding;
(8) it sterilizes: the system after homogeneous being heated to 80~90 DEG C, and is kept for 5~10 minutes;
(9) it is freeze-dried: the material after sterilizing is placed in vacuum freeze drier, drying to its water content reaches 3.8% Below;
(10) tabletting.
Double fermentation is used in the present invention, is yeast fermentation first, and one side yeast is a kind of safe and healthy albumen Matter source, while the zymosan and B family vitamin wherein contained all has exact health-care effect to human body;Another aspect yeast Some Esters are produced in fermentation process, are conducive to the raising of product special flavour and are enriched.Double fermentation is detested using lactic acid bacteria Aerobe fermentation, one side lactic acid bacteria are probiotics, and to adjusting, intestinal health is helpful.Meanwhile lactic acid caused by lactobacillus-fermented Together with the citric acid of addition, so that the pH of Yoghourt peptide sheet articles is reached 3.8~3.9, acidic environment is formed, to effectively protect The stability for protecting GSH, makes glutathione be in reducing condition.Meanwhile acid pH is conducive to the stabilization of product, has anti-corrosion function Energy.
Not only be compounded in Yoghourt peptide piece prepared by the present invention the nutrition such as cow's milk, soybean peptide, corn peptide, maltodextrin at Point, wherein also containing anti-oxidant and alleviation aging using glutathione as the composition of core.Long-term consumption not only can be used as Snack food, supplement and the nutrition for strengthening protein, amino acid.Meanwhile conditioning body may be implemented, it boosts metabolism, clearly Manage the toxic components such as interior free yl, discharge toxic heavy metal.Especially for male, can relieve fatigue, mitigate smoking and It drinks to injury caused by body;For women, this anti-oxidant combination can remove intracorporal free radical, inhibit the heavy of pigment , long-term consumption can protect liver, further achieve the effect that skin whitening.
Specific embodiment
Following embodiment is only intended to illustrate the present invention, application range not for the purpose of limiting the invention.
Embodiment 1
A kind of glutathione Yoghourt peptide piece is made of by weight following raw materials:
The preparation method for preparing 1Kg glutathione Yoghourt peptide piece, includes the following steps:
(1) basestocks mix: toward addition 80g soybean peptide in 3800mL raw milk (dry matter content 11%), 70g corn peptide, In 70g maltodextrin, and 66g white granulated sugar is added;And stir, it boils 10 minutes, with sterilization, is cooled to room Temperature;
(2) yeast aerobic fermentation: to 1) in system in be inoculated with 5% (190mL) Saccharomyces cerevisiae (OD600=300) brewer's wort Culture, aerobic fermentation 8 hours in shaking table or reactor, temperature are 28 DEG C;
(3) lactic acid bacteria anaerobic fermentation: aseptically to 2) in culture in inoculation 10% lactic acid bacteria agent (such as Yoghourt), 42 DEG C of temperature, anaerobic fermentation 10 hours, condense system.Fermentation is that 4.1 or so terminations are fermented to pH.Add appropriate lemon Acid makes its pH reach 3.8 or so;
(4) after-ripening: by 3) obtained in fermentation material in 5 DEG C it is cold set 10 hours, obtain flavor substance therein adequately Accumulation;
(5) be concentrated: by 4) in fermentation material be concentrated by evaporation in lower 60 DEG C of vacuum or reduced pressure to wherein Solid content and reach 40%;
(6) it adds anti-oxidant combination: adding 5% glutathione (50g), 6% vitamin C in Xiang Shangshu concentrate (60g), 0.8% cysteine (8g), 0.8% lipoic acid (8g), are mixed with blender;
(7) homogeneous: by 6) in the material that mixes carry out homogeneous, pressure 35MPa, spent material is more uniform and fine and smooth;
(8) it sterilizes: the system after homogeneous being heated to 90 DEG C, and is kept for 6 minutes;
(9) it is freeze-dried: the material after sterilizing is placed in vacuum freeze drier, drying to its water content reaches 3.8% Below.Material after drying is crushed with mechanical crusher, 80 meshes is crossed, obtains powdered material;
(10) tabletting: will mix after crushing material with appropriate magnesium stearate, carry out dry powder direct tabletting.Tablet press machine is advanced Disinfection and vegetable oil carry out the lubrication of mechanical part.Tableting pressure is 16MPa;
(11) pack: milk tablet is packed using aluminum foil plate or PET bottle.
Embodiment 2
A kind of glutathione Yoghourt peptide piece, preparation method include the following steps:
(1) basestocks mix: 120g soybean peptide, 100g corn being added into 8400mL raw milk (dry matter content 11%) In peptide, 100g maltodextrin, and 138g white granulated sugar is added;And stir, it boils 10 minutes, it is cold with sterilization But to room temperature;
(2) yeast aerobic fermentation: to 1) in system in be inoculated with 5% (420mL) saccharomyces cerevisiae brewer's wort culture, in shaking Aerobic fermentation 8 hours in bed or reactor, then anaerobic fermentation 2 hours, temperature are 28 DEG C;
(3) lactic acid bacteria anaerobic fermentation: aseptically to 2) in culture in inoculation 8% lactic acid bacteria agent (as acid Milk), 42 DEG C of temperature, anaerobic fermentation 9 hours, condense system.Fermentation is that 4.2 or so terminations are fermented to pH.Add appropriate citric acid Make its pH 3.7;
(4) after-ripening: by 3) obtained in fermentation material in 4 DEG C it is cold set 12 hours, accumulate flavor substance therein sufficiently It is tired;
(5) be concentrated: by 4) in fermentation material be concentrated by evaporation in lower 70 DEG C of vacuum or reduced pressure to its Solid content and reach 45%;
(6) it adds antioxidant combination: adding 4% glutathione (80g), 7% vitamin C in Xiang Shangshu concentrate (140g), 0.7% cysteine (14g), 1.2% lipoic acid (24g), are mixed with blender;
(7) homogeneous: by 6) in the material that mixes carry out homogeneous, pressure 38MPa, spent material is more uniform and fine and smooth;
(8) it sterilizes: the system after homogeneous being heated to 85 DEG C, and is kept for 10 minutes;
(9) it is freeze-dried: the material after sterilizing is placed in vacuum freeze drier, drying to its water content reaches 3.8% Below.Material after drying is crushed with mechanical crusher, 80 meshes is crossed, obtains powdered material;
(10) tabletting: will mix after crushing material with appropriate magnesium stearate, carry out dry powder direct tabletting.Tablet press machine is advanced Disinfection and vegetable oil carry out the lubrication of mechanical part.Tableting pressure is 18MPa;
(11) pack: milk tablet is using aluminum foil plate or HDPE bottles of packagings.
Embodiment 3
A kind of glutathione Yoghourt peptide piece is prepared by following raw materials:
450mL milk, 7g soybean peptide, 9g corn peptide, 8g maltodextrin, 5.4g white granulated sugar;4g GSH,8g VC,0.6g Cysteine, 1.5g lipoic acid.
Preparation method is same as above.
Yoghourt peptide tablet raw material selection of the invention has sufficient scientific basis, while taste is sour-sweet.Wherein contain plant Source, animal sources and microbial source a variety of nutrition and health care factors;In particular, in this Yoghourt peptide piece containing it is efficient it is anti-oxidant, Removing toxic substances combination, being eaten for a long time has the double action for removing free radical and anti-aging to human body.
This product is suitable for many people, and balanced nutrition can be provided for children and adolescents;For the alleviation of vast male friend The injury of free radical and the toxic factor caused by smoking, drinking.For women, long-term consumption more can be by eliminating certainly By the effect for realizing skin-whitening the effects of base, protection liver, removing toxic substances.
Present invention gives Yoghourt peptide pieces to make us pleasing function, i.e., anti-oxidant, removing toxic substances and anti-aging.Simultaneously Change the ways and means that people take conventional glutathione health care product.Special specific health-care products are changed to a kind of leisure Food;Functional component is dissolved among snack food, keeps people micro by way of snack food and lasting Take in anti-oxidant, function of detoxification ingredient.On the one hand make the intake of health care product composition more convenient, taste is more pleasant.On the other hand it also drops It is low take health care product excessively concentrate and caused by liver and kidney burden.The absorption and utilization of health-care components are not only improved, simultaneously It makes it easier to be accepted by people.
The specification of this product, 0.5~2g/ piece, 4~1 every time, daily 3~4 times, it is proposed that chew and take.
The sequencing of above embodiments is not only for ease of description, represent the advantages or disadvantages of the embodiments.
It is worth noting that: the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;To the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, and those skilled in the art should manage Solution: it is still possible to modify the technical solutions described in the foregoing embodiments, or to part of technical characteristic into Row equivalent replacement;And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution The spirit and scope of scheme.

Claims (1)

1. a kind of glutathione Yoghourt peptide piece, which is characterized in that be prepared by following raw materials: 300~500mL of milk, soybean 5~10g of peptide, 5~10g of corn peptide, 5~10g of maltodextrin, 5 ~ 8g of white granulated sugar, 2~10g of glutathione, vitamin C 4~ 15g, 0.1~1g of cysteine, 0.2~2g of lipoic acid;
The glutathione Yoghourt peptide piece, preparation method include the following steps:
(1) basestocks mix: soybean peptide, corn peptide, maltodextrin, white granulated sugar being added to milk and stirred, is boiled Boiling 10 minutes, is cooled to room temperature;The dry matter content 11% of the milk;
(2) yeast aerobic fermentation: to 1) in system in inoculation 5% Saccharomyces cerevisiae brewer's wort culture or saccharomyces cerevisiae malt Juice culture, aerobic fermentation 5~8 hours in shaking table or reactor, temperature are 28~30 DEG C;
(3) lactic acid bacteria anaerobic fermentation: aseptically to 2) in culture in inoculation 10% lactic acid bacteria agent, temperature 42 ~45 DEG C, anaerobic fermentation 8~12 hours, condense system;Fermentation is that 4.0 terminations are fermented to pH;Adding citric acid makes its pH 3.8;
(4) after-ripening: by 3) obtained in fermentation material cold set 8~12 hours in 4~8 DEG C;
(5) be concentrated: by 4) in fermentation material under vacuum or reduced pressure 60~80 DEG C be concentrated by evaporation to its Solid content reach 35~ 50%;
(6) it adds anti-oxidant combination: adding glutathione, VC, cysteine, lipoic acid in Xiang Shangshu concentrate, use blender It mixes;
(7) homogeneous: by 6) in the material that mixes carry out homogeneous, 30~40MPa of pressure;
(8) it sterilizes: the system after homogeneous being heated to 80~90 DEG C, and is kept for 5~10 minutes;
(9) be freeze-dried: the material after sterilizing be placed in vacuum freeze drier, it is dry to its water content reach 3.8% with Under;
(10) tabletting.
CN201610090504.6A 2016-02-18 2016-02-18 A kind of glutathione Yoghourt peptide piece and preparation method thereof Active CN105660867B (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0375852A1 (en) * 1988-12-23 1990-07-04 Immunotec Research Corporation Ltd. Biologically active whey protein composition, a method for producing it and use of the composition
CN102599405A (en) * 2012-03-15 2012-07-25 河南省***(集团)有限公司 Binary composite polypeptide chewable tablet
CN103549015A (en) * 2013-11-18 2014-02-05 北京三力源食品商贸有限公司 Ugine sport nutrition milk tea powder and preparation method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0375852A1 (en) * 1988-12-23 1990-07-04 Immunotec Research Corporation Ltd. Biologically active whey protein composition, a method for producing it and use of the composition
CN102599405A (en) * 2012-03-15 2012-07-25 河南省***(集团)有限公司 Binary composite polypeptide chewable tablet
CN103549015A (en) * 2013-11-18 2014-02-05 北京三力源食品商贸有限公司 Ugine sport nutrition milk tea powder and preparation method thereof

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