CN105660387A - Breeding method of sterile line of three-line hybrid rice - Google Patents
Breeding method of sterile line of three-line hybrid rice Download PDFInfo
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- CN105660387A CN105660387A CN201610068407.7A CN201610068407A CN105660387A CN 105660387 A CN105660387 A CN 105660387A CN 201610068407 A CN201610068407 A CN 201610068407A CN 105660387 A CN105660387 A CN 105660387A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a breeding method for transferring a sterile line of three-line hybrid rice by using a Basmati fragrant rice material. According to the method, excellent germplasm resources in the South Asia region are introduced, planting in different regions and different seasons is conducted, tracking improvement is insisted, and the material Pusa derived from an Indian Basmati fragrant rice group is discovered to have good field plant leaf form, good quality, high resistance and certain holding capacity for a wild abortive type sterile line; through continuous multi-generation system selection, a method for searching individual genotype difference from groups is adopted, the screening pressure on large segregation groups is increased, minor restoring genes are eliminated by generations over 8 years, a high-quality maintainer line material Pusa167-12 is identified from the fragrant rice resources in combination with rice quality early-generation screening and taste evaluation, and through continuous pairing backcross with Zhenshan 97A, a high-quality fragrant sterile line Zhongzhe A which has new plant type, good resistance and quality up to ministerial high-quality rice Class 1-2 standards is successfully transferred.
Description
Technical field
The invention belongs to technique for breeding of hybridized rice field, be specifically related to a kind of series of three-series hybrid rice sterile line breeding method.
Background technology
In recent years, along with rice does the raising of development and the people's living standard produced, Rice Quality Problem causes the concern of people day by day, and improving rice quality is pendulum vital task in face of us. According to China's high quality paddy standard, the rice Geological Problems main manifestations of current series of three-series hybrid rice is that head rice rate is low, chalky grain rate is high, chalkiness degree is big, gel consistence is low, Cooking Quality is poor, therefore commercially lacks competitiveness. Big quantity research shows, the main cause affecting hybrid rice quality is sterile line insufficient quality. Current China major part series of three-series hybrid rice is to utilize CMS line Zhenshan 97a, gold-32A, Gang No.46A, five rich A and Xieqingzao A etc. formulated, but the problems such as the Hybrid Rice Combination ubiquity rice quality of these sterile lines preparation is not good enough, peasant's high yield does not increase income, have far from adapted to the development of agricultural production and the living needs of the people. Therefore the practical sterile line that selection-breeding comprehensive agronomy character is excellent, rice is of fine quality, combining ability is strong, good stress resistance, the breeding processed that is prone to produce is the trend of series of three-series hybrid rice development.
Geographical distribution according to Yebai sterile gene, the Yangtze river basin one band most early, middle Xian kind " open country is lost " sterile line is had the holding capacity of sterility. Thus, most domestic breeder utilizes the Yangtze river basin one direct transformation of Xian kind morning with plantation to become sterile line, or keeps system by artificial guarantor's method selection cross processed. The eighties in last century, International Rice Research Institute was bred as the series good quality male sterile lines such as IR58025A, and made cross combination success at country in Southeast Asia's Plant experimental demonstration. But still have following defects that
1, owing to Xian variety and quality most of morning is not good enough, the sterile line rice quality that therefore new transformation becomes is still poor;
2, due to long-term heredity domestication, most rice varieties in low latitudes rice district, South Asia carry Restore gene or minor effect Restore gene, and colony is difficult in Phenotype containing sterile gene or polygenic individual plant, add work difficulty and the intensity of offspring's testcross and screening;
3, male sterile line in ternary hybrid rice hereditary basis relatively narrower, heredity intension do not enrich, lack comprehensive agronomy character is excellent, rice is of fine quality, combining ability is strong, good stress resistance, be prone to crossbreeding production sterile line.
Summary of the invention
The present invention is directed to above-mentioned Problems existing and make improvement, namely it is an object of the invention to provide a kind of series of three-series hybrid rice sterile line breeding method, this method is by utilizing South Asia high-quality resource to capture the transformation halfway technical barrier of sterile line fertility, and then widen the selection-breeding scope and the genetic background of abundant sterile line that keep system, finally select that rice quality is excellent, Comprehensive Traits well particularly produces upper applicable good quality male sterile line.
In order to solve above-mentioned technical problem, the present invention proposes such a series of three-series hybrid rice sterile line breeding method, and the method specifically includes following steps:
Step 1, introduce the quality germplasm of South Asia region, purpose is had to carry out its strange land, the plantation of different season, and adhere to the innovation of long-term breeding material and follow the tracks of improvement, select that not only field strain Leaf pattern is good, quality is excellent, resistance, and wild abortion type sterile series is had certain holding capacity come from India Basmati perfume (or spice) rice group material Pusa;
Step 2, by the Systematic selection to Pusa continuous multi-generation, the method taking to find idiotype difference in colony, strengthen separating big colony screening pressure, minor effect Restore gene is eliminated by generation, in conjunction with field rice food flavor evaluation, from 11767 parts of resources, identify extra best best keep based material Pusa167-12;
Step 3, by the continuous pairs backcross transformation of Pusa167-12 Yu Zhenshan 97a, final transformation goes out Zhejiang A in the high-quality odor type sterile line that plant type is new, better resistance, main quality reach superior rice 1-2 grade standard promulgated by the ministries or commissions of the Central Government.
Further, described step 2 is specially and with coming from India Basmati perfume (or spice) rice group material Pusa hybridization, Zhenshan 97a is obtained first filial generation F1, from F1In generation, selects, no less than 20000 pairs of paired test crosses of individual plant material, continuous test cross 8 years, eliminate minor effect by generation and recover individual plant, finally select Pusa167-12.
Synchronizing improvement by sterile line and restorer quality, bound fraction indica-japonica heterosis utilizes, the Good quality hybrid rice of selection-breeding " straight after front pine " novel strain type. Achievement innovation and practicality is strong, efficiently solves the contradiction of hybrid paddy rice high yield and high-quality.
Beneficial effects of the present invention:
First: the breeding technique of middle Zhejiang A, break the Regional Distribution traditional view of three-line indica hybrid rice Rescued virus, overcome and utilize low latitudes rice varieties transformation sterile line to bring the not thorough technical barrier of offspring's fertility, middle Zhejiang A sterile plant rate is 100%, pollen sterility degree reaches 99.99%, and bagging self-fruitful rate is 0.012%. Being confined to for a long time from the Yangtze river basin early Xian kind, screening keeps the range expansion of system to South Asia low latitudes, enriches the genetic background of sterile line;
Second: in high-quality odor type sterile line, Zhejiang A is domestic first and utilizes the sterile line that South Asia India Basmati odor type grain quality rice parent is material transformation. Owing to having imported the fine quality character of Basmati perfume (or spice) rice, 10 rice matter indexs of middle Zhejiang A (B) are formed sediment except straight chain part content (14.2%) and portion mark high-quality secondary standard (15%) quite except, all other indexs all portions of meeting or exceeding mark superior rice 1-2 grade standard; In affecting the processing quality (head rice rate) of rice, exterior quality (transparency and chalkiness degree) and Cooking Quality (gel consistence) etc., more existing main CMS line improves significantly, and particularly " gel consistence " index closely-related with rice eating-quality obtains bigger lifting compared with domestic similar sterile line;
3rd: flowering habit is good, stigma exposing ratio is high. Stigma white, stigma exposing ratio 71.32%, wherein double; two exposing ratios 37.37%, generally outcrossing seed-setting rate is more than 45%;
4th: middle Zhejiang A belongs to the slow ripe type of Xian in temperature sensitive type, duration from seeding to heading 87-91 days, and strain Leaf pattern is excellent, be suitable in preparation, evening New Hybrid Rice Combination. Blast resisting in middle Zhejiang A, average 3.0 grades of leaf pestilence is the highest 5.0 grades; Average 2.0 grades of fringe pestilence is the highest 5.0 grades. Middle sense bacterial leaf-blight is average 6.0 grades, the highest 7.0 grades. Through the popularization and application of various places in recent years, land for growing field crops pest and disease damage occurs light, and the resistance of banded sclerotial blight, brown paddy plant hopper is better;
5th: the excellent series of combination in middle Zhejiang achieves Zhejiang Province and the update of periphery province Hybrid Rice Varieties, improve the quality that China three is Indica Hybrid rice, it has also become the domestic ternary hybrid rice combination relatively having power of influence.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.
Fig. 1 is the technology of the present invention route map;
Detailed description of the invention
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments. Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art obtain under not making creative work premise, broadly fall into the scope of protection of the invention.
As it is shown in figure 1, be a kind of embodiment of the present invention, a kind of series of three-series hybrid rice sterile line breeding method, the method specifically includes following steps:
Step 1, introduce the quality germplasm of south east asia, purpose is had to carry out its strange land, the plantation of different season, and adhere to the innovation of long-term breeding material and follow the tracks of improvement, select that not only field strain Leaf pattern is good, quality is excellent, resistance, and wild abortion type sterile series is had certain holding capacity come from India Basmati perfume (or spice) rice group material Pusa;
Step 2, obtains first filial generation F by Zhenshan 97a with coming from India Basmati perfume (or spice) rice group material Pusa hybridization1, from F1In generation, selects, no less than 20000 pairs of paired test crosses of individual plant material, continuous test cross 8 years, eliminate minor effect Restore gene by generation, in conjunction with field rice food flavor evaluation, from 11767 parts of resources, identify extra best best keep based material Pusa167-12;
Step 3, by the continuous pairs backcross transformation of Pusa167-12 Yu Zhenshan 97a, final transformation goes out Zhejiang A in the high-quality odor type sterile line that plant type is new, better resistance, main quality reach superior rice 1-2 grade standard promulgated by the ministries or commissions of the Central Government.
The breeding technique of middle Zhejiang A, break the Regional Distribution traditional view of three-line indica hybrid rice Rescued virus, overcome and utilize low latitudes rice varieties transformation sterile line to bring the not thorough technical barrier of offspring's fertility, middle Zhejiang A sterile plant rate is 100%, pollen sterility degree reaches 99.99%, and bagging self-fruitful rate is 0.012%. Being confined to for a long time from the Yangtze river basin early Xian kind, screening keeps the range expansion of system to South Asia low latitudes, enriches the genetic background of sterile line;
Zhejiang A in high-quality odor type sterile line, is domestic first and utilizes the sterile line that South Asia India Basmati odor type grain quality rice parent is material transformation. Owing to having imported the fine quality character of India perfume (or spice) rice, 10 rice matter indexs of middle Zhejiang A (B) are formed sediment except straight chain part content (14.2%) and portion mark high-quality secondary standard (15%) quite except, all other indexs all portions of meeting or exceeding mark superior rice 1-2 grade standard;In affecting the processing quality (head rice rate) of rice, exterior quality (transparency and chalkiness degree) and Cooking Quality (gel consistence) etc., more existing main CMS line improves significantly, and particularly " gel consistence " index closely-related with rice eating-quality obtains bigger lifting (table 1) compared with domestic similar sterile line;
The comparison of Zhejiang A and existing main CMS line Major Grain matter index in table 1
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any amendment of making, equivalent replacement, improvement etc., should be included within protection scope of the present invention.
Claims (2)
1. a series of three-series hybrid rice sterile line breeding method, it is characterised in that the method specifically includes following steps:
Step 1, introduce the quality germplasm of South Asia region, purpose is had to carry out its strange land, the plantation of different season, and adhere to the innovation of long-term breeding material and follow the tracks of improvement, select that not only field strain Leaf pattern is good, quality is excellent, resistance, and wild abortion type sterile series is had certain holding capacity come from India Basmati perfume (or spice) rice group material Pusa;
Step 2, by the Systematic selection to Pusa continuous multi-generation, the method taking to find idiotype difference in colony, strengthen separating big colony screening pressure, minor effect Restore gene is eliminated by generation, in conjunction with field rice food flavor evaluation, from 11767 parts of resources, identify extra best best keep based material Pusa167-12;
Step 3, by the continuous pairs backcross transformation of Pusa167 Yu Zhenshan 97a, final transformation goes out Zhejiang A in the high-quality odor type sterile line that plant type is new, better resistance, main quality reach superior rice 1-2 grade standard promulgated by the ministries or commissions of the Central Government.
2. a kind of series of three-series hybrid rice sterile line breeding method according to claim 1, it is characterised in that described step 2 is particularly as follows: obtain first filial generation F by Zhenshan 97a with coming from India Basmati perfume (or spice) rice group material Pusa hybridization1, from F1Select 20000 to the above paired test cross of individual plant material, continuous test cross 8 years generation, eliminate minor effect by generation and recover individual plant, finally select Pusa167-12.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106508663A (en) * | 2016-10-26 | 2017-03-22 | 内江杂交水稻科技开发中心 | Method for comprehensive cultivation of sterile line of three-line hybrid rice |
| CN106818457A (en) * | 2017-01-22 | 2017-06-13 | 福建农林大学 | A kind of selection of loose panicle type perfume rice CMS line |
Citations (2)
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|---|---|---|---|---|
| CN101595840A (en) * | 2009-07-01 | 2009-12-09 | 中国水稻研究所 | A kind of allusion quotation loses the selection of the wide affine male sterile line of type high stigma exposure rate japonica rice rice |
| CN104686320A (en) * | 2015-04-01 | 2015-06-10 | 内江杂交水稻科技开发中心 | Cultivation method of aromatic high-quality rice sterile line |
-
2016
- 2016-01-29 CN CN201610068407.7A patent/CN105660387A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101595840A (en) * | 2009-07-01 | 2009-12-09 | 中国水稻研究所 | A kind of allusion quotation loses the selection of the wide affine male sterile line of type high stigma exposure rate japonica rice rice |
| CN104686320A (en) * | 2015-04-01 | 2015-06-10 | 内江杂交水稻科技开发中心 | Cultivation method of aromatic high-quality rice sterile line |
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| 童汉华: ""中国水稻所育成米质优、株型新的三系不育系中浙A"", 《HYBRID RICE》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106508663A (en) * | 2016-10-26 | 2017-03-22 | 内江杂交水稻科技开发中心 | Method for comprehensive cultivation of sterile line of three-line hybrid rice |
| CN106818457A (en) * | 2017-01-22 | 2017-06-13 | 福建农林大学 | A kind of selection of loose panicle type perfume rice CMS line |
| CN106818457B (en) * | 2017-01-22 | 2018-10-23 | 福建农林大学 | A kind of selection of loose panicle type perfume (or spice) rice CMS line |
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Application publication date: 20160615 |