CN105638405A - Papaya tissue culture seedling transplanting method - Google Patents
Papaya tissue culture seedling transplanting method Download PDFInfo
- Publication number
- CN105638405A CN105638405A CN201511018537.1A CN201511018537A CN105638405A CN 105638405 A CN105638405 A CN 105638405A CN 201511018537 A CN201511018537 A CN 201511018537A CN 105638405 A CN105638405 A CN 105638405A
- Authority
- CN
- China
- Prior art keywords
- papaya
- tissue culture
- sprout
- transplanting
- culture seedlings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the technical field of fruit tree growing, and particularly relates to a papaya tissue culture seedling transplanting method. The method comprises the steps of 1, taking well cultured papaya tissue culture seedlings out of a culture vessel by clumps, placing the papaya tissue culture seedlings in a container with the bottom and periphery hollowed out, and determining the number of the papaya tissue culture seedlings arranged in the container according to the size of the container; 2, soaking the roots of the papaya tissue culture seedlings in the container completely in disinfectant for 5-10 min, and then taking the papaya tissue culture seedlings out; 3, planting the papaya tissue culture seedlings which are taken out in a culture medium by clumps. By the adoption of the method, the transplantation survival rate of the papaya tissue culture seedlings can be increased, and growth recovery of the papaya tissue culture seedlings is quick; the formula of the disinfectant is reasonable, the possibility of generation of damping-off pathogenic bacteria in the transplanting period of the papaya tissue culture seedlings can be reduced, and growth of roots of the transplanted papaya tissue culture seedlings is facilitated; the adopted culture medium has air permeability and a water retention function, so that survival and growth of the papaya tissue culture seedlings are facilitated.
Description
Technical field
The invention belongs to fruit tree cultivation technical field, be specifically related to the method for transplanting of a kind of papaya sprout.
Background technology
Fructus Caricae (CaricapapayaL.) is also known as Fructus Chaenomelis, breast melon, papaw, and for the torrid zone, the large-scale herbaceos perennial of subtropical evergreen soft wood, evergreen soft wood dungarunga, up to 8-10 rice, tool milk; Stem not branch or sometimes in injury region branch, the stipule trace of tool helical arrangement. Fruit is longer than on tree, outer image melon, therefore named Fructus Chaenomelis. The milk of Fructus Caricae is the Main Ingredients and Appearance making meat tenderizer.
Fructus Caricae all has distribution in tropical and subtropical region, the world. Originate in Mexico south and contiguous middle part, America, be now distributed mainly on the Malaysia in Southeast Asia, Philippine, Thailand, Vietnam, Burma, Indonesia and India and Sri Lanka; In, South America, the West Indies, the Florida of the U.S., Hawaii, Cuba and Australia. China is mainly distributed on the ground such as Guangdong, Hainan, Guangxi, Yunnan, Fujian, Taiwan.
The conventionally employed seed growing of Fructus Caricae, the shortcoming that seed growing has trait depression, survival rate is low. Tissue cultured seedling technology is used widely in Fructus Caricae is planted as a kind of emerging biotechnology. Papaya sprout, in transplanting process, owing to being transplanted to the substrate of planting carried disease germs from aseptic culture medium, owing to substrate is unholiness, carries disease germs to some extent, easily cause the morbidity of root pathogenic bacteria cause rotten, rotten Seedling and reduce the transplanting survival rate of tissue cultured seedling; Traditional method needs before transplanting, through washing Seedling removal culture medium, major part root system damage to be caused to cause plant root dysfunction, and then affect the growth of papaya sprout; The commonly used earth of traditional cultivation substrate, its breathability and water-retaining property are poor, it is difficult to provide suitable moisture and nutrition for papaya sprout, thus affecting the growth of papaya sprout.
It is disclosed in the information of this background section and is merely intended to increase the understanding of the general background to the present invention, and be not construed as admitting or imply in any form that this information structure is for prior art that persons skilled in the art are known.
Summary of the invention
Defect for prior art, it is an object of the invention to provide the method for transplanting of a kind of papaya sprout, the inventive method is transplanted not to be needed Fructus Caricae Seedling is washed Seedling operation, tissue cultured seedling is dipped disinfectant solution, then being colonizated in and take root in substrate, the papaya method for transplanting of the present invention can improve the survival rate of papaya sprout.
The present invention is achieved through the following technical solutions:
The method for transplanting of a kind of papaya sprout, comprises the following steps:
(1) the papaya sprout Cheng Congcong culture vessel cultivated will take out, be placed in the container of bottom and surrounding hollow out, determine, according to container size, the quantity put;
(2) being completely immersed in disinfectant solution by the root of the papaya sprout in whole container, the immersion time is 5��10min, then takes out;
(3) the papaya sprout Cheng Cong further taken out is colonizated in cultivation matrix.
As preferably, the papaya sprout taken out in step (1) does not remove the culture medium of root.
As preferably, the disinfectant solution described in step (2) contains following components by mass percentage: carbendazim 30��40%, agricultural streptomycin 20��40% and 30��40% hymexazol.
As preferably, described disinfectant solution extension rate in use is 1000��2000 times.
As preferably, the cultivation matrix by volume percentage ratio described in step (3) contains following components: peat soil 90%, perlite 5% and Vermiculitum 5%.
Compared with prior art, there is advantages that
1. the method for transplanting of the papaya sprout of the present invention need not wash Seedling when tissue cultured seedling field planting, can saving of work and time, raise labour efficiency.
2. the method for transplanting of the papaya sprout of the present invention can improve the transplanting survival rate of papaya sprout, and tissue cultured seedling growth recovery is fast.
3. the disinfectant solution formula used in the inventive method is reasonable, has both been possible to prevent the generation of papaya sprout nursery stage disease, can promote that again Fructus Caricae is accelerated to take root.
4. the cultivation matrix used in the inventive method has effect of ventilative water conservation, it is easy to the surviving and grow of Fructus Caricae Seedling.
Detailed description of the invention
Below in conjunction with embodiment, the specific embodiment of the present invention is described in detail, it is to be understood that protection scope of the present invention is not by the restriction of detailed description of the invention.
Explicitly indicate that unless otherwise other, otherwise in entire disclosure and claims, term " includes " or its conversion will be understood to comprise the element or ingredient stated at such as " comprising " or " including " etc., and does not get rid of other element or other ingredient.
Embodiment 1:
The method for transplanting of a kind of papaya sprout, comprises the following steps:
(1) by taking out in the papaya sprout Cheng Congcong culture vessel cultivated, being placed in the container of bottom and surrounding hollow out, container is permeable basket, and every basket fills 30 clumps; The papaya sprout taken out does not remove the culture medium of root;
(2) being completely immersed in disinfectant solution by the root of the papaya sprout in whole container, the immersion time is 5min, then takes out; Described disinfectant solution contains following components by mass percentage: carbendazim 30%, agricultural streptomycin 40% and 30% hymexazol;Described disinfectant solution extension rate in use is 1000 times;
(3) the papaya sprout Cheng Cong further taken out is colonizated in cultivation matrix; Described cultivation matrix by volume percentage ratio contains following components: peat soil 90%, perlite 5% and Vermiculitum 5%.
Embodiment 2:
The method for transplanting of a kind of papaya sprout, comprises the following steps:
(1) by taking out in the papaya sprout Cheng Congcong culture vessel cultivated, being placed in the container of bottom and surrounding hollow out, container is permeable basket, and every basket fills 35 clumps; The papaya sprout taken out does not remove the culture medium of root;
(2) being completely immersed in disinfectant solution by the root of the papaya sprout in whole container, the immersion time is 10min, then takes out; Described disinfectant solution contains following components by mass percentage: carbendazim 40%, agricultural streptomycin 20% and 40% hymexazol; Described disinfectant solution extension rate in use is 2000 times;
(3) the papaya sprout Cheng Cong further taken out is colonizated in cultivation matrix; Described cultivation matrix by volume percentage ratio contains following components: peat soil 90%, perlite 5% and Vermiculitum 5%.
Embodiment 3:
The method for transplanting of a kind of papaya sprout, comprises the following steps:
(1) by taking out in the papaya sprout Cheng Congcong culture vessel cultivated, being placed in the container of bottom and surrounding hollow out, container is permeable basket, and every basket fills 25 clumps; The papaya sprout taken out does not remove the culture medium of root;
(2) being completely immersed in disinfectant solution by the root of the papaya sprout in whole container, the immersion time is 8min, then takes out; Described disinfectant solution contains following components by mass percentage: carbendazim 35%, agricultural streptomycin 30% and 35% hymexazol; Described disinfectant solution extension rate in use is 1500 times;
(3) the papaya sprout Cheng Cong further taken out is colonizated in cultivation matrix; Described cultivation matrix by volume percentage ratio contains following components: peat soil 90%, perlite 5% and Vermiculitum 5%.
The papaya sprout that method described in embodiment 1-3 is transplanted, matched group 1-3 be common method for transplanting transplant papaya sprout (matched group 1,2,3 respectively adopt carbendazim, agricultural streptomycin and hymexazol disinfectant solution 1000 times sterilization 30min, then adopting clear water to transplant after carrying out washing Seedling, comparison substrate is common soil). Measure the content of organic matter transplanting cultivation matrix after papaya sprout and saturation moisture capacity by experiment, and the papaya sprout transplanting survival rate after transplanting and Fructus Caricae damping-off sickness rate are investigated, added up, the results detailed in Table 1.
Table 1 use the inventive method to the performance measurement after papaya transplantation of seedlings
As shown in Table 1, the content of organic matter in the cultivation matrix after the method transplanting of the present invention, saturation moisture capacity is used to be above comparison; Use the transplanting survival rate of the papaya sprout after the method transplanting of the present invention far above comparison; Use the damping-off sickness rate of the papaya sprout after the method transplanting of the present invention far below comparison.
In sum, the method for transplanting of the papaya sprout of the present invention can improve the transplanting survival rate of papaya sprout; The disinfectant solution formula that the present invention uses is reasonable, has both been possible to prevent the generation of Fructus Caricae damping-off, can promote that again Fructus Caricae accelerates to take root; The cultivation matrix that the present invention uses has effect of ventilative water conservation, it is easy to the surviving and grow of Fructus Caricae Seedling, and the method for the present invention has highly application value.
The aforementioned description to the specific illustrative embodiment of the present invention illustrates that and the purpose of illustration. These describe and are not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to above-mentioned instruction, it is possible to carry out much changing and change. Purpose exemplary embodiment selected and describe is in that to explain the certain principles of the present invention and practical application thereof, so that those skilled in the art is capable of and utilizes the various different exemplary of the present invention and various different selection and change. The scope of the present invention is intended to be limited by claims and equivalents thereof.
Claims (5)
1. the method for transplanting of a papaya sprout, it is characterised in that comprise the following steps:
(1) the papaya sprout Cheng Congcong culture vessel cultivated will take out, be placed in the container of bottom and surrounding hollow out, determine, according to container size, the quantity put;
(2) being completely immersed in disinfectant solution by the root of the papaya sprout in whole container, the immersion time is 5��10min, then takes out;
(3) the papaya sprout Cheng Cong further taken out is colonizated in cultivation matrix.
2. the method for transplanting of papaya sprout according to claim 1, it is characterised in that the papaya sprout taken out in step (1) does not remove the culture medium of root.
3. the method for transplanting of papaya sprout according to claim 1, it is characterized in that, the disinfectant solution described in step (2) contains following components by mass percentage: carbendazim 30��40%, agricultural streptomycin 20��40% and 30��40% hymexazol.
4. the method for transplanting of papaya sprout according to claim 3, it is characterised in that described disinfectant solution extension rate in use is 1000��2000 times.
5. the method for transplanting of papaya sprout according to claim 1, it is characterised in that the cultivation matrix by volume percentage ratio described in step (3) contains following components: peat soil 90%, perlite 5% and Vermiculitum 5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511018537.1A CN105638405A (en) | 2015-12-30 | 2015-12-30 | Papaya tissue culture seedling transplanting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511018537.1A CN105638405A (en) | 2015-12-30 | 2015-12-30 | Papaya tissue culture seedling transplanting method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105638405A true CN105638405A (en) | 2016-06-08 |
Family
ID=56478397
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201511018537.1A Pending CN105638405A (en) | 2015-12-30 | 2015-12-30 | Papaya tissue culture seedling transplanting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105638405A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111657126A (en) * | 2020-05-09 | 2020-09-15 | 福建省农业科学院果树研究所 | Substrate for improving seedling rate of papaya seedlings and seedling method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739341A (en) * | 2004-08-24 | 2006-03-01 | 易自力 | Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum |
| CN1911000A (en) * | 2006-08-24 | 2007-02-14 | 广州市果树科学研究所 | Method for speedly breeding new variety of papaya |
| CN101578960A (en) * | 2009-06-24 | 2009-11-18 | 华中农业大学 | Method for reducing aerial root of tissue culture seedling of hydrangea |
| CN103733932A (en) * | 2013-12-04 | 2014-04-23 | 张家港市塘桥果艺园 | Papaya seedling culturing and planting method |
| CN104920056A (en) * | 2015-06-30 | 2015-09-23 | 蒋清校 | Cultivation method for improving papaya quality |
| CN105104053A (en) * | 2015-07-24 | 2015-12-02 | 太仓市丰缘农场专业合作社 | Pawpaw high-yield planting method |
-
2015
- 2015-12-30 CN CN201511018537.1A patent/CN105638405A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739341A (en) * | 2004-08-24 | 2006-03-01 | 易自力 | Aseptic seedling tissue culturing and test tube seedling hardening off and transplating technology for anthurium andraeanum |
| CN1911000A (en) * | 2006-08-24 | 2007-02-14 | 广州市果树科学研究所 | Method for speedly breeding new variety of papaya |
| CN101578960A (en) * | 2009-06-24 | 2009-11-18 | 华中农业大学 | Method for reducing aerial root of tissue culture seedling of hydrangea |
| CN103733932A (en) * | 2013-12-04 | 2014-04-23 | 张家港市塘桥果艺园 | Papaya seedling culturing and planting method |
| CN104920056A (en) * | 2015-06-30 | 2015-09-23 | 蒋清校 | Cultivation method for improving papaya quality |
| CN105104053A (en) * | 2015-07-24 | 2015-12-02 | 太仓市丰缘农场专业合作社 | Pawpaw high-yield planting method |
Non-Patent Citations (1)
| Title |
|---|
| 胡颂平: "《植物细胞组织培养技术》", 31 December 2014 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111657126A (en) * | 2020-05-09 | 2020-09-15 | 福建省农业科学院果树研究所 | Substrate for improving seedling rate of papaya seedlings and seedling method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105103856B (en) | A kind of is suitable for the method for cultivating Moringa oleifera in northern area | |
| CN101790912B (en) | Method for sprouting oleaster seeds and raising seedlings | |
| CN103609223A (en) | Method Paris polyphylla var.chinensis through gibberellin and wet sand stratification | |
| CN110089336B (en) | Tending method for endangered mangrove plant sonneratia pseudosea seedling | |
| CN104380994A (en) | Bletilla striata large-scale high-yield cultivation method | |
| CN105103928A (en) | Method for acclimatizing and transplanting bletilla striata tissue culture seedlings | |
| CN104041412A (en) | Rapid propagation method for tissue culture of Guizhou hemiboea cavaleriei | |
| CN105230484A (en) | Rapid breeding method of rhizoma polygonati | |
| CN103340046B (en) | Method for promoting halogeton sativus seeds to rapidly germinate | |
| CN105144905A (en) | Acer ginnala seed germination treatment method | |
| CN104871679A (en) | Balance dormancy breaking and seedling cultivation method of cerasus campanulata seeds | |
| CN102783415A (en) | Method for conservation in vitro of cassava germplasm resources with stability and high efficiency | |
| CN105830698A (en) | Seedling hardening method for sweet potato tissue-cultured test-tube plantlets | |
| CN103782785B (en) | A kind of plum blossom seeds cultivation method | |
| CN103444309B (en) | Gibberellin and filter paper combine the method making paris polyphylla seed quick-speed germination | |
| CN105594500A (en) | Palm container seedling culture method | |
| CN103125393A (en) | Aseptic seeding and rapid tissue-culture propagation method of Callicarpa nudiflora Hook.ex Am | |
| CN107996242A (en) | A kind of Moringa container seedling culture method | |
| CN106879275A (en) | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination | |
| CN110122302A (en) | A kind of oat seedlings breeding method | |
| CN105493951A (en) | Transplanting method of tissue-culture derived banana plantlets | |
| CN104542221A (en) | Method for cultivating manchurian ash light-medium bagged seedlings by discarded agaric mushroom sticks | |
| CN105103710A (en) | Method for treating seeds for promoting germination of oilseed rape and improving cold resistance of oilseed rape | |
| CN105638405A (en) | Papaya tissue culture seedling transplanting method | |
| CN106171366A (en) | A kind of hair cuttage rapid propagating method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160608 |
|
| RJ01 | Rejection of invention patent application after publication |