CN105612996B - A kind of Chinese torreya cuttage breeding method - Google Patents
A kind of Chinese torreya cuttage breeding method Download PDFInfo
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Abstract
A kind of method of Chinese torreya cutting propagation, technical field of plant asexual propagation, it is characterised in that: mainly include the following steps: 1) slotting machine preparation；2) cuttings acquires；3) cuttings processing and cuttage；4) cuttage final-period management；5) it transplants；6) final-period management is transplanted.The technical solution provided through the invention, Chinese torreya cutting plantation is up to 96.67%, and lateral root number is more, well developed root system；It is counted within 14 months after cuttage transplantation of seedlings, survival rate is up to 91.67%, shoot length 4.53cm, well developed root system, solve the problems, such as that Chinese torreya cuttage is not easy period length of taking root, take root, method is simple and easy, at low cost, can be realized Chinese torreya fast and effectively vegetative propagation, promotes to quickening Chinese torreya superior genotypes, reasonable utilize of its child care and afforestation popularization and resource is promoted to have very important realistic meaning.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, specially a kind of method of Chinese torreya cutting propagation.
Background technique
Chinese torreya (Torreya grandis' Merrillii ') be taxaceae (Taxaceae) Chinese torreya category (Torreya
Arn.) Chinese torreya (Torreya grandisFort. ex Lindl) in a cultivated form, be that China is distinctive rare dry
Fruit, resource scarcity, life cycle is long, is the multiduty high-quality warps such as collection fruit is used, oil is used, is medicinal, material is used, the ornamental, ecology of greening
Help tree species monoid.The edible nutritive value of Chinese torreya seed is very high, there is protein abundant and various trace elements；It is processed into dry
Fruit can be used as ticbit, while be also rare traditional Chinese medicine, and sweet in flavor and neutral in nature, being used as medicine has effects that desinsection, anti-inflammatory moistening lung.This
Outside, the bark of Chinese torreya can obtain through refining industrial tannin extract.Its grain of wood is straight and hardness is moderate, is shipbuilding, building, sleeper, furniture
And the excellent material of technique engraving.Chinese torreya landscape effect is good, and the four seasons are evergreen, tree performance is graceful；Ecological functions are strong, and seedlings or saplings are resistance to
Yin is suitable in thin underwood planting, and afforestation is few to original vegetation destruction, is the fine tree species of Lower value stand transformation.Chinese torreya tree crown
Dense, hayashishita fallen leaves thickness, leaf is easy to rot, all significant to water conservation, improvement soil.
The development that the high economic value of Chinese torreya seed has pushed Chinese torreya to produce at present, planting scale increases considerably, right
The demand of nursery stock increasingly increases, but the more propagations by grafiting of Chinese torreya, and only making stock with Chinese torreya will at least cultivate 2 years, commonly uses 2+2(2
The culture of+2 years graftings of stock culture) grafting nursery stock, the production cycle is relatively long, and has becoming for culture tree transplanted at present
Gesture, and cutting propagation is not yet widely applied in production.
In recent years, the economic value high due to Chinese torreya, the research report in relation to Chinese torreya is quite a few, primarily focuses on cell
It learns and technology, tissue cultures etc. is fostered in histology, molecular labeling, constituent analysis, propagation by grafiting technology, cultivation.With regard to Chinese torreya cuttage
Technology also has relevant report, such as: patent document (publication number: CN 104838829A) discloses a kind of nothing of high-quality edible Chinese torreya
Property quick-breeding method, directly with choose branch make cuttings, cuttings cuttage is cultivated to seedling on seedbed, eliminates traditional Chinese torreya
The process of grafting vernalization breeding grafting, substantially reduces seedling cost, and shorten the result time of nursery stock.Chen Shumao
(2001) the raw hardwood cutting of Chinese torreya 1a, next year survival rate 0% are carried out mid-April；Late June cuttage, next year survival rate 65%.Guo Wei
Magnificent (2002) are on July, the middle ten days carries out cuttage, use 1000mgL-1ABT6 root-inducing powder (GGR6) processing, works as annual survival rate
92%, next year retention 83%.Wan little Jin etc. (2006) is the study found that spring Chinese torreya 1a life branch cutting, survival rate 62%；Summer
With current year raw semi-lignified branch cuttage, 0.05% ABT1 root-inducing powder of cardinal extremity or methyl α-naphthyl acetate (NAA) 1000mgL-1Solution
10s is dipped in, 55d or so is taken root, planting percent 80%.These tests have obtained certain PRELIMINARY RESULTS, have centainly to Chinese torreya cuttage
Good effect, but Chinese torreya cuttage root-taking difficulty could not be solved preferably always as the technical problem of this field, in fact
Because how undeveloped Chinese torreya cuttage seeding root system is, it is especially a lack of healthy and strong lateral root, cuttage survival rate usually will cause a kind of " illusion ",
I.e. branch survives on surface, and substantial branch lower part does not take root or seldom takes root, when using this cuttage transplantation of seedlings
Also easily dead.
Summary of the invention
For technological deficiency existing in the prior art, the object of the present invention is to provide a kind of sides of Chinese torreya cutting propagation
Method solves the technical problem that cuttage root-taking is difficult, transplanting survival rate is low.
The technical solution adopted in the present invention is as follows:
A kind of Chinese torreya cuttage breeding method, it is characterised in that: mainly include the following steps:
1) slotting machine prepares: building slotting machine in greenhouse, the sunshade net of light transmittance 50% is laid with above cutting bed, slotting machine is built-in
Fill out cutting medium；
Slotting machine is 0.3m (W) × 1.1m (L) × 0.2m (H) foam tank composition, is fixed with iron wire, and bottom has rhone
With drainage hole；0.03% liquor potassic permanganate is sprayed in foam rooved face before cuttage and covers diaphragm seal disinfection 14d；
The matrix loaded in slotting machine is the mixed-matrix of peat, perlite and vermiculite, volume proportion are as follows: peat: perlite:
Vermiculite=1:1:1；Slotting machine bottom first spreads the haydite drainage blanket that one layer of 3cm thickness radius is 1-2cm when loading matrix；2d sprays before cuttage
0.125% carbendazim (25% effective content) solution is applied, is carried out disinfection to matrix；
2) cuttings acquires: summer acquires current year raw semi-lignified branch and one section of base portion band from 10-12a raw Chinese torreya female plant
The branch (hereinafter referred to as band heel branch) of the raw branch section of 3cm long 2a makees cuttings, notch is wrapped up with wet towel immediately, to prevent cuttings from losing
Water, oxidation, it is desirable that the branch of robust growth, no disease and pests harm and mechanical damage；Remove the blade in the raw branch section of 2a；Entire cuttings leaching
Bubble sterilizes about 2min in 0.125% carbendazim solution, and it is spare to be placed on shady place after draining；Used band heel branch is raw for 2a
Retain the current-year branch of 3 tool terminal buds in branch section；
Preferably, cuttings specification is preferably rugosity 0.2-0.3cm, length 13-15cm, wherein long 14cm, thick 0.27cm
Effect is best；
3) cuttings processing and cuttage: using sharp cutter truncation cuttings base portion before cuttage, pay attention to keeping smooth cut smooth, use
2.5mg·L-1Marking cuttage after the dedicated taking root liquid of 3721 cuttage of allusion quotation impregnates cuttings base portion 15s, in the matrix of slotting machine, cuttage depth is
The raw branch section of 2a is placed in matrix；It waters immediately after cuttage, guarantees that cuttings base portion comes into full contact with matrix；
4) cuttage final-period management: mainly including water management, fertilising and the prevention and control of plant diseases, pest control；
Water management method is as follows: cuttage initial stage, carries out moisturizing temperature control to cuttings using auto spraying equipment and sunshade net,
Spray intervals are that spraying 30s stops 5min, runing time 8:30-17:00, keep slotting machine humidity 70-90%, 33 DEG C of temperature or less；
After cuttings callus forms and is flourishing, adjustment spray intervals are that spraying 30s stops 20min, runing time 8:30-17:00；
It applies fertilizer as follows with pest control method: 0.125% carbendazim solution is sprayed every 10-15d, with 0.01% maneb
Zinc wheel is used, to prevent generating resistance；After callus forms and is flourishing, 0.2% potassium dihydrogen phosphate and the mixing of 0.15% urea are sprayed
Liquid is as foliar fertilizer, interval 10-15d；
5) it transplants: cuttage seeding being transplanted in 5 months after cuttage；Matrix is peat: perlite: vermiculite=1:1:1(V/V/
V), container is non-woven fabrics Seedling bag；Preferably, preferably carrying out point processing of being completely cured before transplanting；
6) it transplants final-period management: applying No. 5 Controlled Release Fertilizer (n-p-k ratios of Osmocote (OSMOCOTE) after transplanting March in next year
Example is 14-13-13), every basin about 2g.
Using above technical scheme, Chinese torreya cutting plantation is up to 96.67%, and lateral root number is more, well developed root system；Cuttage transplantation of seedlings
It is counted within 14 months afterwards, survival rate is up to 91.67%, shoot length 4.53cm, well developed root system.The present invention solves Chinese torreya skewer
The slotting problem for being not easy to take root, the period of taking root is long, method is simple and easy, at low cost, and it is fast and effectively asexual numerous to can be realized Chinese torreya
It grows, quickening Chinese torreya superior genotypes is promoted, its child care is promoted to utilize with afforestation popularization and the reasonable of resource with extremely important
Realistic meaning.
Specific embodiment
The present invention will be further described in detail below with reference to specific embodiments.Mentioned concentration not specified (NS),
For the conventional concentration representation method of this field, i.e. mass percentage concentration.
Embodiment 1
Chinese torreya cuttage mainly includes the following steps:
1) slotting machine prepares: cuttage place is located at Linan City of Zhejiang Province (29 ° of 56'-30 ° of 23' of north latitude, 118 ° of 51'- of east longitude
119 ° of 52') Zhejiang A & F University's subtropical forest cultivate National Key Laboratory base greenhouse.It is built on greenhouse cutting bed
1.8m high brandreth is laid with the sunshade net of light transmittance about 50%, is equipped with auto spraying equipment.Slotting machine be 0.3m (W) × 1.1m (L) ×
The foam tank of 0.2m (H) forms, and is fixed with iron wire, and bottom has rhone and drainage hole.0.03% is sprayed in foam rooved face
Liquor potassic permanganate simultaneously covers diaphragm seal disinfection 14d；
2) cutting medium: matrix is peat (0-10mm, Denmark Pindstrup), vermiculite (5-8mm), perlite (2-
4mm), by peat: perlite: vermiculite=1:1:1(V/V/V) mixing match.Slotting machine bottom first spreads one layer of 3cm thickness when loading matrix
Radius is the haydite drainage blanket of 1-2cm.2d sprays 0.125% carbendazim (25% effective content) solution before cuttage, carries out to matrix
Disinfection.The disinfection of slotting machine and matrix can be reduced the generation of cuttings pest and disease damage, guarantee the survival rate of Chinese torreya cuttage；
3) cuttings acquisition and production: in morning early July or at dusk in the Chinese torreya cultivation base in Zhejiang A & F University campus
Ground acquires from 10-12a raw Chinese torreya female plant and does the cuttings (annual shoots of 3 tool terminal buds of reservation in the raw branch section of 2a with heel branch
Item is about 14cm, slightly about 0.27cm), it is desirable that the branch of robust growth, no disease and pests harm and mechanical damage.The processing of cuttings is as far as possible
It is completed in 2h after acquisition, and wraps up cuttings with wet towel at any time in treatment process, to prevent cuttings dehydration, oxidation.Removal heel
The following blade in portion.Entire cuttings, which is immersed in 0.125% carbendazim (25% effective content) solution, sterilizes about 2min, is placed on after draining
Shady place is spare；
4) cuttage: using sharp cutter truncation cuttings base portion before cuttage, pays attention to keeping smooth cut smooth, notch is exposed to sky
The shorter the time the better in gas, uses 2.5mgL-1It marks the dedicated taking root liquid of 3721 cuttage of allusion quotation (Zhengzhou Biao Dian Science and Technology Development Co., Ltd.)
Cuttage after immersion cuttings base portion 15s.Cuttage depth unsuitable too deep, about 3cm, 6 × 6cm of spacing, and compress around compacting cuttings
Matrix is watered immediately after cuttage, guarantees that cuttings base portion comes into full contact with matrix；
5) cuttage final-period management:
Water management: cuttage initial stage carries out moisturizing temperature control, spray intervals to cuttings using auto spraying equipment and sunshade net
Stop 5min, runing time 8:30-17:00 for spraying 30s, keeps slotting machine humidity 70-90%, 33 DEG C of temperature or less.Cuttings callus
After organizing the formation of simultaneously flourishing (about 1 month), adjustment spray intervals are that spraying 30s stops 20min, runing time 8:30-17:00；
Fertilising and the prevention and control of plant diseases, pest control: 0.125% carbendazim (25% effective content) solution is sprayed every 10-15d, with 0.01%
Mancozeb wheel is used, to prevent generating resistance.Plum rain season will be reinforced preventing and treating, and divulge information in time, prevent cuttings pest and disease damage from occurring.More
Injured tissue forms and after flourishing (about 1 month), sprays 0.2% potassium dihydrogen phosphate and 0.15% urea mixed solution as foliar fertilizer,
It is 10-15d every the phase, has and improve cuttage seeding resistance, promotes the effect of growth；
6) transplant: (December current year) transplants cuttage seeding 5 months after cuttage.Matrix is peat: perlite: vermiculite=
1:1:1(V/V/V), container is 7cm × 15cm non-woven fabrics Seedling bag (beauty plants bag).Point processing of being completely cured is carried out before transplanting；
7) it transplants final-period management: applying No. 5 Controlled Release Fertilizer (n-p-k ratios of Osmocote (OSMOCOTE) after transplanting March in next year
Example is 14-13-13).
Using the program, Chinese torreya cutting plantation is up to 96.67%, and lateral root number is more, well developed root system；14 after cuttage transplantation of seedlings
It month is counted, survival rate is up to 91.67%, shoot length 4.53cm, well developed root system.
Embodiment 2
1 experimental field overview
Experimental field it is located at Linan City of Zhejiang Province (29 ° of 56'-30 ° of 23' of north latitude, 118 ° of 51'-119 ° of 52' of east longitude) Zhejiang agricultural
The greenhouse and laboratory in university's subtropical forest cultivation National Key Laboratory base.1.8m high-speed rail is built on greenhouse cutting bed
Frame is laid with the sunshade net of light transmittance about 50%, is equipped with auto spraying equipment.
2 materials and methods
2.1 test materials and processing
Morning or dusk in the Chinese torreya cultivation base in Zhejiang A & F University campus, acquire from 10-12a raw Chinese torreya female plant
Current year raw robust growth, the branch (hereinafter referred to as common branch) of no disease and pests harm and mechanical damage.The processing of cuttings is being adopted as far as possible
It is completed in 2h after collection, and wraps up cuttings with wet towel at any time in treatment process, to prevent cuttings dehydration, oxidation.Cuttings rugosity
0.2-0.4cm, length 13-15cm, degree of lignification is close, and removes the 1/3 of the total blade of base portion.Entire cuttings is immersed in
About 2min is sterilized in 0.125% carbendazim (25% effective content) solution, it is spare to be placed on shady place after draining.With sharp knife before cuttage
Piece chamfers cuttings base portion, pays attention to keeping smooth cut smooth, and the shorter the time the better in air for notch exposure, and it is raw to carry out external source
Long regulator processing.Cuttage depth be cuttings total length 1/3, generally 5cm, 4 × 5cm of spacing, and compress compacting cuttings around
Matrix, guarantee cuttings base portion come into full contact with matrix.
2.2 test method
2.2.1 influence of the cuttage season to Chinese torreya cuttage root-taking
Four seasons cuttage, spring cuttage were carried out on April 2nd, 2015, and summer cuttage was carried out on July 8th, 2014, autumn skewer
It is inserted in progress on October 6th, 2014, cuttage in winter was carried out on January 30th, 2015.It is unified to use using current-year branch
300mg·L-1GGR6(also known as double gill root-inducing powders；The production of Beijing Ai Bidi Biotechnology Co., Ltd) impregnate 20min.Often
A season is a processing, and every processing is 30 plants, is repeated 3 times.
2.2.2 exogeneous growth regulator handles the influence to Chinese torreya cuttage
Summer acquires Chinese torreya semi-wooden scion and carries out cuttage, with (1) GGR6；(2) α-naphthylacetic acid (α-
Naphthaleneacetic, NAA)；(3) 3 kinds of exogeneous growths of indolebutyric acid (Indole-3-butyric acid, IBA) are adjusted
Agent (A) is handled, and every kind of hormone uses 3 kinds of concentration (B), i.e. (1) 100mgL-1；(2) 200mgL-1；(3) 500mgL-1.Cuttings handles 20min, and impregnating cuttings 20min with (CK) clear water is control.Share 10 processing combinations, every processing combination 30
Strain, is repeated 3 times.
2.2.3 influence of the selection of cuttings with processing to Chinese torreya cuttage
Summer is acquired respectively with heel branch (with embodiment 1), the raw semi-lignified branch of the current year from Chinese torreya tree crown top
(being about 8.4cm, slightly about 0.24cm) makees cuttings, carries out cuttage in greenhouse；Summer acquire respectively band heel branch (be about 11.3cm,
Thick about 0.22cm), current year raw common semi-lignified branch make cuttings (being about 10.5cm, slightly about 0.27cm), in the controlled environment chamber
Interior carry out cuttage.All cuttings 2.5mgL-1It marks the dedicated taking root liquid of 3721 cuttage of allusion quotation and (marks the limited public affairs of allusion quotation scientific and technological development in Zhengzhou
Department) impregnate cuttage after cuttings base portion 15s.Every processing is 10 plants, 3 repetitions.
2.2.4 influence of the different substrates to Chinese torreya cuttage root-taking
Autumn acquires current year Chinese torreya stiff wood and carries out cuttage, cuttings specification almost the same (long 14-15cm, thick 0.24-
0.30cm), there are peat (0-10mm, Denmark Pindstrup), vermiculite (5-8mm), perlite (2-4mm), rice husk for test matrix
Charcoal (Dalian ninety percent).It matches for totally five kinds, i.e. (A) peat: perlite: vermiculite=1:1:1(V/V/V, similarly hereinafter)；(B) peat: pearl
Rock: vermiculite=1:2:1；(C) peat: perlite: vermiculite=1:4:1；(D) peat: perlite: vermiculite=1:8:1；(E) peat: precious
Zhu Yan: vermiculite: rice hull carbon=1:3:1:1.Matrix was prepared on October 5th, 2014, then carried out cuttage.One is managed during cuttage
It causes, such as sprays at the same time.Every kind of substrate composition is regarded as 1 processing, every processing are 30 plants, 3 repetitions.Use agricultural
Environment monitor (TNHY-D-16, Hangzhou Instrument Ltd. of Top, precision 3%) is about 3.5 months after substrate preparation cuttage
Water supply in media assay is carried out when 2:00 in afternoon.
2.2.5 different lower cuts handle the influence to Chinese torreya cuttage root-taking
Spray is acquired in summer, truncation, chamfering process are carried out to cuttings base portion respectively, uniformly use 300mgL-1GGR6
Cuttage after immersion 20min.
2.2.6 separate sources branch cultivates the influence to Chinese torreya growth of cuttage seedlings
By (A) band heel branch, (B) tree crown top branch, (C) tree crown middle and lower part branch, (D) common branch cutting breeding 5
A month and the more consistent cuttage seeding of growth are transplanted with no treatment, and transplanting medium and container are with embodiment 1, to 9
It is total to the nursery stock cultivated 14 months after month namely since the cuttage and carries out Root morphology, biomass, increment measurement, and counts nursery stock
Storage rate.Every seedling randomly selects 1 plant, 3 repetitions.
The measurement of 2.3 rooting inhibitors and statistics
After cuttage, state observation of taking root is carried out every 7-10d, records callus apparition, adventitious root apparition, every time
Every processing takes 3 plants, carefully plants back after observation.It takes root in largely processing, main root extends and carries out system of taking root when there is lateral root
Meter, investigation statistics rooting rate, callus rate, the death rate.The adventitious root of 5 plants of representative each cuttings of statistics is selected in every processing
Number, i.e. main root number, lateral root number, 3 repetitions, totally 15 plants；With ruler measurement, main root is long, lateral root is long, and calculates Rooting index and (take root
Rate × mean elements × average root long), average total root long (cm) of a length of single plant of average root.
Root morphology observation: taking each processing to cultivate the cuttage transplanted seedling that the time is 14 months, and cuttings root system is carried out number
Change scanning (Epson perfection V700 photo, Japan) to obtain complete root system image, then with matched
It is analysis system software WinRHIZO_ProV2007b(Regent Instrument Inc. Canada) analysis Root morphology,
Obtain the parameters such as root long, root surface area, root volume.
Biomass estimation is based on the dry biomass measurement after drying (80 DEG C, 72 h to constant weight), and every processing choosing has generation
5 plants of table are measured.Root/shoot ratio=Underground biomass dry weight/Aboveground Biomass of Young dry weight.
2.4 data statistics and analysis
Institute's measured data is arranged using Excel2003, data are for statistical analysis with SPSS18.0 software.Percentage
It carries out square root arcsin transformation and square root conversion respectively with enumeration data, then carries out variance analysis；Use duncan's new multiple range method
(Duncan method) carries out Multiple range test to the result of variance analysis significant difference.
3 results and analysis
Influence of the 3.1 cuttage seasons to Chinese torreya cuttage root-taking
The cuttage of the current-year branch four seasons starts statistics of taking root after growing lateral root.Spring 163d after cuttage, summer is in cuttage
170d afterwards, autumn 339d after cuttage, winter, the 225d after cuttage carried out statistics of taking root.Observation shows Chinese torreya four seasons
(table 1) can be taken root by carrying out cuttage.Spring and summer carry out cuttage, and the cuttage rooting time is less than fall and winter.Spring and summer
The cuttings root elongation phase in season is respectively 83d, 65d after cuttage, and autumn, winter root elongation occur in 168d, 145d respectively
(table 1).
It is perhaps survived after cuttings cuttage or dead, the cuttings survived has again takes root and only form callus and non-shape
The case where at root, the sum of percentage of three are 100%.In one-way analysis of variance, there are done on the basis of significant difference
Multiple range test shows that spring and summer cutting plantation are significantly higher than fall and winter (P < 0.05), and spring rooting rate is
31.67%, summer 31.11%, respectively higher than fall and winter 8.34%, 23.34% and 7.78%, 22.78%(table 2).It only forms
Callus is callus rate without the cuttings statistics taken root.Summer cuttage cuttings callus rate is significantly higher than three season of winter in spring and autumn (P <
0.05), up to 61.11%, higher than other three seasons 56.66%, 45.55%, 56.11%(table 2 respectively).Survival rate be rooting rate and
The sum of callus rate, summer (92.22%) > autumn (38.89) > spring (36.67%) > winter (13.33%).The death rate then winter
(86.67%) in > spring (63.33%) > autumn (61.11%) > summer (7.78%), spring and autumn is close, with the death rate of summer cuttage
It is minimum.
The main root number of each cuttings of four seasons cuttage is without apparent difference, but fall and winter cuttage lateral root number is significantly higher than the spring
Season and summer (table 2).Spring and Autumn Cutting, Rooting index are significantly higher than winter (P < 0.05) (table 2).But from when cultivating
Between on from the point of view of, spring cuttage is less than autumn, and Autumn Cutting seedling cultivates time 176d(table 2 more than spring).It is dry from aerial part
From the point of view of quality, spring to winter, cuttings aerial part dry mass is gradually incremented by, winter highest, reaches 1.14g, and spring is minimum, is
0.62g, two season of summer and autumn is respectively 0.87g, 0.99g.Spring cuttage, root/shoot ratio are significantly higher than other the third quaters, up to 0.14, divide
Not than improving 133.33%, 40%, 180%(P < 0.05 in three season of summer and autumn winter).
It can be seen that four seasons cuttage, summer cuttage is preferable, and survival rate is significantly higher than other seasons, and the death rate is low, when taking root
Between it is shorter, rooting rate is higher.
Influence of the 3.2 exogeneous growth regulators processing to Chinese torreya cuttage root-taking
Two-way analysis of variance the result shows that, exogeneous growth regulator type (A), concentration (B) are raw to Chinese torreya summer cuttage
Root has a significant impact (P < 0.05) (table 3).A is to rooting rate, callus rate, the main root number of each cuttings, lateral root number, finger of taking root
Number, root dry mass, root/shoot ratio have extremely significant influence (P < 0.01) (table 3).Side of the B to rooting rate, callus rate, each cuttings
Radical has extremely significant influence (P < 0.01) (table 3).The interaction (A × B) of dual factors to the rooting rate of cuttings, callus rate,
Upper part dry mass, root/shoot ratio have extremely significant influence (P < 0.01), have a significant impact (P < to the main root number of each cuttings
0.05), remaining each index does not make significant difference (table 3) then.
Exogeneous growth regulator type shows rooting rate NAA > IBA > to the Multiple range test that Chinese torreya cuttage rooting influences
GGR6(P < 0.05) (table 4), callus rate GGR6 > IBA > NAA is inversely proportional with rooting rate.The main root number of each cuttings, lateral root
Number, Rooting index NAA > IBA > GGR6.Root dry mass, root/shoot ratio NAA > IBA > GGR6, the wherein close (P of NAA and IBA
< 0.05) (table 4).Therefore, Chinese torreya summer cuttage exogeneous growth regulator type selection NAA is preferable.
Exogeneous growth modifier concentration shows rooting rate and callus rate to the Multiple range test that Chinese torreya cuttage rooting influences
100mg·L-1With 200 mgL-1Processing is significantly higher than 500mgL-1It handles (P < 0.05) (table 5).The lateral root of each cuttings
Several 200 > 100mgL of then 500 >-1It handles (P < 0.05).Root/shoot ratio 100mgL-1Processing is significantly higher than 500mgL-1Place
It manages (P < 0.05), then difference is not significant (P < 0.05) (table 5) for remaining each index.The exogeneous growth regulator rooting rate of low concentration
With root/shoot ratio height, callus rate is also high, but lateral root number is substantially less than the exogeneous growth regulator of high concentration.Therefore, Chinese torreya summer skewer
It inserts, exogeneous growth modifier concentration selects 100mgL-1Or 200mgL-1Processing is preferable.
Comprehensive analysis, 100mgL-1Or 200mgL-1NAA processing Chinese torreya cuttings it is preferable, rooting rate is respectively
64.44%, 55.56%, it is higher by 25.18%, 16.3% respectively than clear water control (CK), the main root number of each cuttings, is taken root at lateral root number
Index is higher (table 6).
Influence of the selection of 3.3 cuttings with processing to Chinese torreya cuttage
One-way analysis of variance the result shows that, different type cuttings has remarkable effect (P < to summer Chinese torreya cuttage root-taking
0.05) (table 7) makees cuttings cuttage, lateral root number, Rooting index, ground and the root of each cuttings with heel branch under greenhouse experiment
Portion's dry mass is significantly higher than the branch (P < 0.05) from Chinese torreya tree crown top, remaining each index such as rooting rate, death rate is then not
It is not significant (P < 0.05) (table 7) with difference between cuttings.Use band heel branch cutting preferable in greenhouse, rooting rate 96.67%, side
Radical is 13.83, and root dry mass is high, and root growth is preferable.
Influence of 3.4 different substrates to Chinese torreya cuttage root-taking
One-way analysis of variance shows that the water content difference of different ratio matrix is significant (P < 0.05) (table 8), A > E > B
> C > D；With the increase of perlite ratio, water penetration gradually increases A to D, and water supply in media content gradually decreases；A water supply in media
Content highest is that 19.53%, D is minimum, is 4.83%.
One-way analysis of variance also indicates that simultaneously, different ratio matrix to Chinese torreya cutting plantation, callus rate, take out tip rate,
Cuttings lateral root number, Rooting index, root dry mass, root/shoot ratio influence significant difference (P < 0.05) (table 9).Rooting rate handles A
It is significantly higher than other processing (P < 0.05), A > D > C > B > E, wherein C and D is close；Callus rate A and C are significantly higher than its elsewhere
It manages (P < 0.05), C > A > B > D > E, wherein A and C is close；Survival rate A > C > D > B > E；The death rate then with survival rate phase
Instead；It takes out tip rate A and is significantly higher than other processing (P < 0.05), A > C > D > B > E, wherein C and D is close.Lateral root number D is significantly high
In other processing, and difference is not significant between the processing of the main root number of each cuttings.Rooting index A and D are significantly higher than B and C(P <
0.05).Root dry mass and root/shoot ratio processing D are significantly higher than other processing, and difference is not shown between the processing of aerial part dry mass
It writes (P < 0.05).
Therefore, processing A is best, and rooting rate, survival rate, pumping tip rate, lateral root number, Rooting index are higher, and handle D and take root
Index and root cap are relatively high, handle E rooting rate and survival rate is minimum.
The processing test of 3.5 cutting base portions
One-way analysis of variance shows the truncation of cuttings base portion and beveling, the main root of rooting rate, callus rate, each cuttings
Significant difference (P < 0.05) (table 10) between number, the processing of lateral root number, Rooting index, root dry mass, root/shoot ratio.Truncation cuttage root-taking
Rate is 61.67%, improves 30.56% than beveling, the lateral root number on the main root number of each cuttings, each main root, Rooting index point
97.42%, 106.57%, 1049.50% is not improved, and survival rate beveling is higher than truncation.Truncation is improved compared to beveling, root/shoot ratio
50%.Therefore, better than beveling, rooting rate, the main root number of each cuttings, lateral root number, Rooting index, root/shoot ratio are aobvious for base portion truncation
It writes and improves.
3.6 separate sources branches cultivate the influence to Chinese torreya growth of cuttage seedlings
Summer Chinese torreya cuttage discovery, the branch rooting rate and quality of rooting of three types are higher, respectively band heel branch, tree
Label portion, middle and lower part branch.Therefore after transplanting 9 months, these processing cuttage seeding root systems and growing state are observed with very heavy
The meaning wanted.
The cuttage seeding that Chinese torreya separate sources branch is cultivated, significant difference between Root Characters different type seedling after cultivating 14 months
(P < 0.05) (table 11).For A class seedling other than root average diameter, remaining Root Characters index is significantly higher than other processing；B and D
The root system surface area of class seedling, root system volume are significantly higher than C；Root average diameter B is significantly higher than C；Root system overall length, tip of a root number are then poor
Different not significant (P < 0.05).Therefore, A root traits are best, and B and D take second place, and handle worst.
Several A that sprout are substantially less than other types, and storage rate and pumping tip rate difference is not significant (P < 0.05).Increment D is significant
Higher than other types；On the ground and dry mass A in root is significantly higher than other types, and C is significantly less than B and D；Root/shoot ratio A and C is significantly high
In B and D(P < 0.05).
Comprehensive analysis, the ground A, root biomass and root/shoot ratio highest, root traits are good, but the number that sprouts is few, takes out tip rate
It is low；B root character is taken second place；D aerial amount is high, and ground biomass is also high, but root traits are poor, and root/shoot ratio is minimum；The ground C,
Root biomass is minimum, and root traits are poor, but root/shoot ratio is high.
Claims (2)
1. a kind of Chinese torreya cuttage breeding method, it is characterised in that: mainly include the following steps:
1) slotting machine prepares: building slotting machine in greenhouse, the sunshade net of light transmittance 50% is laid with above cutting bed, loads skewer in slotting machine
Insert matrix；
2) cuttings acquires: summer acquires current year raw one section of 3cm of semi-lignified branch and base portion band from 10-12a raw Chinese torreya female plant
The branch of the raw branch section of long 2a makees cuttings, wraps up notch with wet towel immediately, to prevent cuttings dehydration, oxidation, it is desirable that robust growth,
The branch of no disease and pests harm and mechanical damage；Remove the blade in the raw branch section of 2a；Entire cuttings is immersed in 0.125% carbendazim solution
Middle disinfection about 2min, it is spare to be placed on shady place after draining；In the raw branch section of the long 13-15cm of cuttings, thick 0.2-0.3cm, and the 2a
Retain the current-year branch of 3 tool terminal buds；
3) cuttings processing and cuttage: using sharp cutter truncation cuttings base portion before cuttage, pay attention to keeping smooth cut smooth, use
2.5mg·L-1Marking cuttage after the dedicated taking root liquid of 3721 cuttage of allusion quotation impregnates cuttings base portion 15s, in the matrix of slotting machine, cuttage depth is
The raw branch section of 2a is placed in matrix；It waters immediately after cuttage, guarantees that cuttings base portion comes into full contact with matrix；
4) cuttage final-period management: mainly including water management, fertilising and the prevention and control of plant diseases, pest control；
5) it transplants: cuttage seeding being transplanted in 5 months after cuttage；Matrix is peat by volume: perlite: vermiculite=1:1:1,
Container is non-woven fabrics Seedling bag；
6) it transplants final-period management: applying No. 5 Controlled Release Fertilizers of Osmocote, every basin 2g after transplanting March in next year；
Slotting machine in the step 1) is width x length × high 0.3m × 1.1m × 0.2m foam tank composition, is fixed with iron wire, bottom
Portion has rhone and drainage hole；It sprays 0.03% liquor potassic permanganate in foam rooved face before cuttage and covers diaphragm seal and disappear
Malicious 14d；
The matrix loaded in slotting machine in the step 1) is the mixed-matrix of peat, perlite and vermiculite, volume proportion are as follows: mud
Charcoal: perlite: vermiculite=1:1:1；Slotting machine bottom first spreads the haydite drainage blanket that one layer of 3cm thickness radius is 1-2cm when loading matrix；
2d sprays 0.125% carbendazim solution before cuttage, carries out disinfection to matrix；
Water management method is as follows in the step 4): cuttage initial stage, is carried out using auto spraying equipment and sunshade net to cuttings
Moisturizing temperature control, spray intervals are that spraying 30s stops 5min, runing time 8:30-17:00, keep slotting machine humidity 70-90%, temperature
33 DEG C or less；After cuttings callus forms and is flourishing, adjustment spray intervals are that spraying 30s stops 20min, runing time 8:
30-17:00；
It applies fertilizer in the step 4) as follows with pest control method: spraying 0.125% carbendazim solution every 10-15d, with
0.01% Mancozeb wheel is used, to prevent generating resistance；After callus forms and is flourishing, spray 0.2% potassium dihydrogen phosphate and
0.15% urea mixed solution is as foliar fertilizer, interval 10-15d；
The step 5) carries out point processing of being completely cured before transplanting.
2. a kind of Chinese torreya cuttage breeding method according to claim 1, it is characterised in that: cuttings is long in the step 2
14cm, thick 0.27cm, and retain the current-year branch of 3 tool terminal buds in the raw branch section of 2a.
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| CN106069562A (en) * | 2016-06-17 | 2016-11-09 | 枞阳县白云生态园林有限责任公司 | A kind of method of quickly breeding Fructus Vitis viniferae cutting seedling |
| CN106212236A (en) * | 2016-08-03 | 2016-12-14 | 江西省·中国科学院庐山植物园 | A kind of Rhododendron fortuneilindl． Callus formation and technology taken root of promoting |
| CN108093902B (en) * | 2017-12-01 | 2020-11-10 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting cuttage rooting of kiwi fruits by using momordica grosvenori lixivium |
| CN108419550A (en) * | 2018-03-20 | 2018-08-21 | 武龙保 | A kind of Chinese yew old branch high-survival rate cuttage breeding method |
| CN112930891A (en) * | 2021-02-04 | 2021-06-11 | 浙江省林业科学研究院 | Method for full-exposure cutting seedling raising of Chinese torreya |
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