CN105420354B - 基于InDel标记的常规稻品种淮稻5号和淮稻18号的鉴定方法 - Google Patents
基于InDel标记的常规稻品种淮稻5号和淮稻18号的鉴定方法 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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Abstract
Description
编号 | 引物对名称 | 正向引物序列 (5'->3') | 反向引物序列(5'->3') |
1_1 | chr01-1 | gttgttcagtcaaaagtttcagc | tccttatgtgaagtggaagtataac |
1_2 | chr01-2 | atcggtagcactaaatctttcc | gatagggttttaggtttttcgag |
2_1 | chr02-1 | gtaagactagctgtttcaatcacag | gtatgaggtatagacatgggagaac |
2_2 | chr02-2 | agttgagatgaatagctagatggag | aacttgaggaggatcggtatc |
3_1 | chr03-1 | ggatcaagtagcacgataagc | acctattggttgtgtgtcttgtag |
3_2 | chr03-2 | tgtctcattcagagtatggagttc | ttagattggagctatagttgaggac |
4_1 | chr04-1 | cattgacttttcaacacattgg | cataaatttccaggccatatactac |
4_2 | chr04-2 | aacaaccgaaaggtatatgacac | gaatccttaaaattgtaccgtagtg |
5_1 | chr05-1 | gaactcttgctcttcatagaaaatg | taatactcatcctctccgatcc |
5_2 | chr05-2 | cctaaaacctttatcccaaagc | gatcatgtagaaatgaacggc |
6_1 | chr06-1 | cacttagacagcacaaaaatatacc | actaattaaagtcgacacatgacag |
6_2 | chr06-2 | ctaatttgctacacttctgtcgtc | tgtaaagcatatagcaccaagttac |
7_1 | chr07-1 | gtgtgccagttatatttcactttag | ctgagttgacattgagaaaacaatc |
7_2 | chr07-2 | gcgatcaaagtctataaaacatctg | atatctgcagtagctgtcaaaaag |
8_1 | chr08-1 | ttctattaaagtcacaaggctcac | aatacaggcatatctaaaggttgtc |
8_2 | chr08-2 | gtaaagtttgtgtgttcaaaggag | agtcgagtacgtctatcacacatc |
9_1 | chr09-1 | tagggtgttgtataaatggtattgc | ccattagtttacatgtggaatgtc |
9_2 | chr09-2 | cacatttcgttgtcttatctgag | atctttgacctggcactcttac |
10_1 | chr10-1 | ggtaaaactaatcatatcgaggaag | tactgttatatgttggatggtgaac |
10_2 | chr10-2 | gacgatcttaaactaaaacatgagc | atccaacaacaggatattactatgg |
11_1 | chr11-1 | agtgagaaccaatgttgagatg | cattataaaagaacccatagagagc |
11_2 | chr11-2 | aactaaacccataagtcagtgtagc | ctattcactactcacctaccatgtc |
12_1 | chr12-1 | atcctatatcatgtgtttcacacc | atatcactttgacctttttccctc |
12_2 | chr12-2 | cttagcctcttctgttgaaaattg | gcaactgaaactacatgactagaag |
编号 | 引物对名称 | 正向引物序列 (5'->3') | 反向引物序列(5'->3') |
1-1 | chr01-1 | gttgttcagtcaaaagtttcagc | tccttatgtgaagtggaagtataac |
1-2 | chr01-2 | atcggtagcactaaatctttcc | gatagggttttaggtttttcgag |
2-1 | chr02-1 | gtaagactagctgtttcaatcacag | gtatgaggtatagacatgggagaac |
2-2 | chr02-2 | agttgagatgaatagctagatggag | aacttgaggaggatcggtatc |
3-1 | chr03-1 | ggatcaagtagcacgataagc | acctattggttgtgtgtcttgtag |
3-2 | chr03-2 | tgtctcattcagagtatggagttc | ttagattggagctatagttgaggac |
4-1 | chr04-1 | cattgacttttcaacacattgg | cataaatttccaggccatatactac |
4-2 | chr04-2 | aacaaccgaaaggtatatgacac | gaatccttaaaattgtaccgtagtg |
5-1 | chr05-1 | gaactcttgctcttcatagaaaatg | taatactcatcctctccgatcc |
5-2 | chr05-2 | cctaaaacctttatcccaaagc | gatcatgtagaaatgaacggc |
6-1 | chr06-1 | cacttagacagcacaaaaatatacc | actaattaaagtcgacacatgacag |
6-2 | chr06-2 | ctaatttgctacacttctgtcgtc | tgtaaagcatatagcaccaagttac |
7-1 | chr07-1 | gtgtgccagttatatttcactttag | ctgagttgacattgagaaaacaatc |
7-2 | chr07-2 | gcgatcaaagtctataaaacatctg | atatctgcagtagctgtcaaaaag |
8-1 | chr08-1 | ttctattaaagtcacaaggctcac | aatacaggcatatctaaaggttgtc |
8-2 | chr08-2 | gtaaagtttgtgtgttcaaaggag | agtcgagtacgtctatcacacatc |
9-1 | chr09-1 | tagggtgttgtataaatggtattgc | ccattagtttacatgtggaatgtc |
9-2 | chr09-2 | cacatttcgttgtcttatctgag | atctttgacctggcactcttac |
10-1 | chr10-1 | ggtaaaactaatcatatcgaggaag | tactgttatatgttggatggtgaac |
10-2 | chr10-2 | gacgatcttaaactaaaacatgagc | atccaacaacaggatattactatgg |
11-1 | chr11-1 | agtgagaaccaatgttgagatg | cattataaaagaacccatagagagc |
11-2 | chr11-2 | aactaaacccataagtcagtgtagc | ctattcactactcacctaccatgtc |
12-1 | chr12-1 | atcctatatcatgtgtttcacacc | atatcactttgacctttttccctc |
12-2 | chr12-2 | cttagcctcttctgttgaaaattg | gcaactgaaactacatgactagaag |
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CN201510804539.7A CN105420354B (zh) | 2015-11-20 | 2015-11-20 | 基于InDel标记的常规稻品种淮稻5号和淮稻18号的鉴定方法 |
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CN201510804539.7A CN105420354B (zh) | 2015-11-20 | 2015-11-20 | 基于InDel标记的常规稻品种淮稻5号和淮稻18号的鉴定方法 |
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CN105420354B true CN105420354B (zh) | 2018-12-25 |
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CN109666758B (zh) * | 2019-02-14 | 2023-03-07 | 四川省农业科学院经济作物育种栽培研究所 | 基于InDel标记的道地川芎鉴定用引物及鉴定方法 |
CN113308563A (zh) * | 2021-05-28 | 2021-08-27 | 扬州大学 | 一种快速发展澳洲野生稻特异性分子标记的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102031253A (zh) * | 2010-12-16 | 2011-04-27 | 复旦大学 | 用一粒稻谷鉴定籼稻和粳稻的分子标记方法 |
CN104789671A (zh) * | 2015-04-10 | 2015-07-22 | 上海交通大学 | 基于InDel标记的杂交稻品种交源优69的鉴定方法 |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102031253A (zh) * | 2010-12-16 | 2011-04-27 | 复旦大学 | 用一粒稻谷鉴定籼稻和粳稻的分子标记方法 |
CN104789671A (zh) * | 2015-04-10 | 2015-07-22 | 上海交通大学 | 基于InDel标记的杂交稻品种交源优69的鉴定方法 |
Non-Patent Citations (1)
Title |
---|
新一代遗传标记——InDel研究进展;孙宽 等;《法医学杂志》;20130430;第29卷(第2期);134-139、143页 * |
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