CN105410403A - Preparation method of novel food calling constipation-resisting fermented feed - Google Patents
Preparation method of novel food calling constipation-resisting fermented feed Download PDFInfo
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- CN105410403A CN105410403A CN201510891494.1A CN201510891494A CN105410403A CN 105410403 A CN105410403 A CN 105410403A CN 201510891494 A CN201510891494 A CN 201510891494A CN 105410403 A CN105410403 A CN 105410403A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract
The invention belongs to the technical fields of feeds, enzyme preparations and microorganisms and particularly relates to a preparation method of a novel food calling constipation-resisting fermented feed. The novel food calling constipation-resisting fermented feed is prepared by mixing and blending barley, peas, alfalfa meals, molasses and water in proportions, adding saccharomyces cerevisiae strain liquid, lactobacillus plantarum strain liquid, microorganism protease, xylanase and glucanase, carrying out solid anaerobic fermentation enzymolysis and drying. The novel food calling constipation-resisting fermented feed prepared by the preparation method is rich in micro-molecular nutrients such as soluble fiber, small peptide, lactic acid, yeast protein, vitamins and probiotics, and has the characteristics of food calling, constipation resistance, amino acid balance, low anti-nutritional factor content and the like.
Description
Technical field
The invention belongs to feed, enzyme preparation and microbial technology field, be specifically related to the preparation method of a kind of novel food calling, anti-constipation fermented feed.
Background technology
China is populous nation, Ye Shi aquaculture big country.2014, the animal husbandry output value reached 29,250 hundred million yuan, and meat, birds, beasts and eggs, milk and output of aquatic products reach 8,539 ten thousand tons, 2,894 ten thousand tons, 3,725 ten thousand tons and 5,421 ten thousand tons respectively.Along with the development of aquaculture, feed industrial development speed is surprising, and within 2014, national commercial feed output reaches 1.97 hundred million tons, wherein, mixed feed 1.69 hundred million tons, concentrated feed 2,151 ten thousand tons, additive premix 6,410,000 tons, wherein pig feed output 8,616 ten thousand tons.Expect 12 ends, national feed production capacity will reach 3.5 hundred million tons, and output will reach 2.5 hundred million tons.
With regard to pig-breeding, pig-breeding big country of China.Within 2014, China live pig delivers 7.35 hundred million for sale, sow livestock on hand more than 4,500 ten thousand, and pork output reaches 5,671 ten thousand tons, needs nearly 2.0 hundred million tons of pig feed actual year, and wherein sow feed is up to more than 2,000 ten thousand tons.
In sow breeding production, constipation has a strong impact on sow reproductive performance.Sow generation constipation, the lighter can cause sow to lose the appetite, lassitude, and nutrition intake is not enough, cause stillborn foetus, miscarriage, difficult labour and postpartum agalasisa disease etc.Severe one can cause sow to digest and assimilate disorder, insanity, and appetite is absolutely useless, does not rise sleepingly, occasionally has heating.The sow physique that the course of disease is long is weaker, and last physique seriously dies of exhaustion and dies.Therefore, how to prevent in recent years or slow down prevention of sow constipation and receive much concern.
Pea another name cassiae nodosae,semen, garden pea, Mai Dou, also known as honey pea, sweet beans, green soya bean, Shanghai claims " Slight Cold beans " in the neighbourhood.Pea is nutritious, containing protein, fat, and carbohydrate, crude fibre, carrotene, vitamin B1, vitamin B2, calcium, phosphorus, sodium, iron etc.The materials such as the only wooden fork acid contained by pea, gibberellin and the solidifying element of plant, have anti-inflammation, strengthen metabolic function.Containing the dietary fiber compared with horn of plenty in garden pea and bean seedlings, can constipation be prevented, have gut purge effect.
Clover gross protein value is up to 18%-24.8%, and protein contains the amino acid of more than 20 kinds, comprises whole essential amino acids of humans and animals, and some rare amino acids, and wherein the content of lysine is 1.06%-1.38%.Clover also containing the nonprotein nitrogen that a large amount of ruminant domestic animal utilizes, accounts for 33% of clover nitrogen pool.In Alfalfa leaf protein, all kinds of amino acid content is close to animal protein, the composition of amino acid such as lysine, tryptophan is comparatively balanced, its nutritive value and feeding effect are all suitable with fish meal, and higher than soya-bean cake, peanut cake etc., have and significantly increase milk cow body weight and milk yield, improve milk matter, reduce food consumption rate, the feeding effect that the conversion ratio of raising feed etc. are good is a kind of good vegetable protein sources that can regenerate.Use alfalfa hay feeding cow, significantly can strengthen the ability of rumen microorganism synthetic protein, improve the milk yield of cow and the persistence of lactation, reduce the butterfat percnetage of milk.This is because Alfalfa product can balance each seed amino acid of ruminant domestic animal, and provide vitamin and high quality fiber element, make ruminal protein content reach 58% ~ 60% of gross protein, thus greatly improve the result of property of alfalfa protein utilization ratio.In addition in Alfalfa also containing abundant vitamin and mineral matter, be safe, the most most economical crude protein green feed of milk cow.
Barley carbohydrate containing 63.4%, protein 10.2%, dietary fiber 9.9%, also containing B family vitamin etc.Fructus hordei germinatus, mainly containing two kinds of amylase, transforms carbohydrase.Barley also contains maltose, dextrin, B family vitamin, phosphatide, glucose etc.The dietary fiber that in barley, rich conjunction two kinds is important: Soluble Fiber and non-solubility fiber, wherein Soluble Fiber can promote intestines peristalsis, and non-solubility fiber contributes to digestion, and the two all can Constipation.
Molasses are byproducts of sugar industry, be a kind of thickness, pitchy, in semifluid object.Molasses contain a small amount of thick protein, be generally 3%-6%, belong to nonprotein nitrogen class more, as ammonia, acid amides and nitrate etc., and amino-acid nitrogen only accounts for 38%-50%, and nonessential amino acid is as more in asparatate, content of glutamic acid, and therefore protein biological value is lower, but asparatate and glutamic acid are delicious amino acid, therefore for can stimulating animal appetite greatly in animal feed.
Fermented feed is the good feed manufacturing processing method of sow.Feed is through the not only degradable ANFs that ferments, but also small molecule bioactive material can be produced, as little peptide, lactic acid, vitamin, probio and unknown somatomedin etc., but also can promote lactation, be the best substitute of fish meal, PURE WHEY.
Therefore, the present invention intends fermenting to barley, pea, alfalfa meal and molasses, the novel food calling of Development and Production, anti-constipation fermented feed.
Summary of the invention
The present invention is intended to prevent prevention of sow constipation, promote the functions such as lactation, there is provided one to be rich in the Small molecular nutrition such as Soluble Fiber, little peptide, lactic acid, Yeast protein, vitamin and probio, there is novel food calling, the anti-constipation fermented feed of the features such as food calling, anti-constipation, amino acid balance and ANFs content are low.
The technical solution adopted in the present invention is:
A preparation method for novel food calling, anti-constipation fermented feed, step is as follows:
(1), barley, pea, alfalfa meal and molasses, water are mixed modified in proportion, obtained fermentation material wet basis;
(2) in fermentation material wet basis, add saccharomyces cerevisiae bacterial classification fluid strain liquid, Lactobacillus plantarum strain liquid, microbial protease, zytase, dextranase;
(3), solid anaerobic digestion enzymolysis;
(4), obtained novel food calling, anti-constipation fermented feed is dried.
Further, the weight ratio of water in step (1), barley, pea, alfalfa meal and molasses is 100:1 ~ 300:2 ~ 600:2 ~ 600:2 ~ 600.
Preferably, the weight ratio of water in step (1), barley, pea, alfalfa meal and molasses is 100:50 ~ 100:30 ~ 80:30 ~ 80:15 ~ 30.
Further, in step (2), the weight ratio of fermentation material wet basis, saccharomyces cerevisiae bacterial classification fluid strain liquid, Lactobacillus plantarum strain liquid is 100:0.5 ~ 20:0.5 ~ 20; In step (2), the addition of microbial protease, zytase, dextranase is 0.01-1000U/g, 0.005-500U/g, 0.005-500U/g substrate.
Preferably, in step (2), the weight ratio of fermentation material wet basis, saccharomyces cerevisiae bacterial classification fluid strain liquid, Lactobacillus plantarum strain liquid is 100:2 ~ 8:3 ~ 15; In step (2), the addition of microbial protease, zytase, dextranase is 100-300U/g, 50-150U/g, 30-80U/g fermentation material wet basis.
Further, in step (3), fermentation, hydrolysis temperature are 10 ~ 60 DEG C, and fermentation, enzymolysis time are 1 ~ 260h, and the initial pH that ferments is 3-9.
Preferably, in step (3), fermentation, hydrolysis temperature are 25 ~ 35 DEG C, and fermentation, enzymolysis time are 48 ~ 84h, and the initial pH that ferments is 5.0-6.5.
Beneficial effect of the present invention is: novel food calling prepared by the present invention, anti-constipation fermented feed, be rich in the Small molecular nutrition such as Soluble Fiber, little peptide, lactic acid, Yeast protein, vitamin and probio, there is the features such as food calling, anti-constipation, amino acid balance and ANFs content are low.
Detailed description of the invention
Below with reference to embodiment, the present invention is described in detail.
Embodiment 1
20kg pea, 40kg barley, 30kg alfalfa meal and 10kg molasses, 40kg water are mixed modified in proportion, obtained fermentation material wet basis. in fermentation material wet basis, add the addition (180U/g, 80U/g, 50U/g substrate wet basis) of 5kg saccharomyces cerevisiae bacterial classification fluid strain liquid, 8kg Lactobacillus plantarum strain liquid and 0.12kg microbial protease, 0.04kg zytase, 0.06kg dextranase, under 28 ~ 32 DEG C and pH5.8-6.2 condition, after fermentation, enzymolysis time 72h, dry the obtained novel food calling of 98.5kg, anti-constipation fermented feed.
Embodiment 2
15kg pea, 30kg barley, 55kg alfalfa meal and 15kg molasses, 30k water are mixed modified in proportion, obtained fermentation material wet basis. in fermentation material wet basis, add the addition (200U/g, 100U/g, 80U/g substrate wet basis) of 4kg saccharomyces cerevisiae bacterial classification fluid strain liquid, 5kg Lactobacillus plantarum strain liquid and 0.15kg microbial protease, 0.05kg zytase, 0.05kg dextranase, under 30 ~ 32 DEG C and pH6.0-6.2 condition, after fermentation, enzymolysis time 72h, dry the obtained novel food calling of 96kg, anti-constipation fermented feed.
Embodiment 3
200kg pea, 300kg barley, 400kg alfalfa meal and 120kg molasses, 350kg water are mixed modified in proportion, obtained fermentation material wet basis. in fermentation material wet basis, add the addition (250U/g, 70U/g, 60U/g substrate wet basis) of 40kg saccharomyces cerevisiae bacterial classification fluid strain liquid, 60kg Lactobacillus plantarum strain liquid and 0.16kg microbial protease, 0.04kg zytase, 0.06kg dextranase, under 28 ~ 32 DEG C and pH5.8-6.2 condition, after fermentation, enzymolysis time 72h, dry the obtained novel food calling of 960kg, anti-constipation fermented feed.
Embodiment 4
100kg pea, 500kg barley, 400kg alfalfa meal and 150kg molasses, 360kg water are mixed modified in proportion, obtained fermentation material wet basis. in fermentation material wet basis, add the addition (250U/g, 70U/g, 60U/g substrate wet basis) of 50kg saccharomyces cerevisiae bacterial classification fluid strain liquid, 50kg Lactobacillus plantarum strain liquid and 0.16kg microbial protease, 0.05kg zytase, 0.04kg dextranase, under 28 ~ 32 DEG C and pH5.8-6.2 condition, after fermentation, enzymolysis time 72h, dry the obtained novel food calling of 970kg, anti-constipation fermented feed.
Embodiment 5
200kg pea, 200kg barley, 600kg alfalfa meal and 100kg molasses, 500kg water are mixed modified in proportion, obtained fermentation material wet basis. in fermentation material wet basis, add the addition (180U/g, 50U/g, 40U/g substrate wet basis) of 30kg saccharomyces cerevisiae bacterial classification fluid strain liquid, 40kg Lactobacillus plantarum strain liquid and 0.12kg microbial protease, 0.06kg zytase, 0.08kg dextranase, under 28 ~ 32 DEG C and pH5.8-6.2 condition, after fermentation, enzymolysis time 72h, dry the obtained novel food calling of 965kg, anti-constipation fermented feed.
One, the novel food calling of embodiment 1 preparation, the feeding effect of anti-constipation fermented feed
Plant branch, pig farm one in the special agriculture and animal husbandry Development Co., Ltd of Jiangxi Gree and choose body condition, parity, body weight and the expected date of childbirth close healthy Duroc milking sow 160, be divided into 4 groups at random, often organize 5 repetitions, each repetition 8 sows, to study the actual feeding effect of fermented feed, i.e. control group, compound sugar group, 10% fermented feed group, 20% fermented feed group.Test and terminate to during piglet wean in 21 days 108 days from gestation.
4 groups of test sucking pig basal diet compositions are all identical with nutrition.Contrast A group neither adds compound sugar, does not also add fermented feed; Contrast B group interpolation 3% compound sugar, but do not add fermented feed; 10% fermented feed group adds 10% fermented feed; 20% fermented feed group adds 20% fermented feed.Refer to table 1.
Test sow house is roof double-pitch type, cement flooring, and ventilation and lighting is better, natural lighting on daytime, ventilation, night lights illumination, normal immunological and management.Pig house temperature controls to and is not less than 15 DEG C, and humid control is 55-65%.Accomplish that environmental condition is consistent, with at a draft supporting member's management, free choice feeding and drinking-water as far as possible.
Table 1 tests daily ration composition and nutrition
Note: 4% premix contains calcium formate, calcium monohydrogen phosphate, salt, lysine, methionine, vitamin, trace element and medicine etc.
The novel food calling of table 2, anti-constipation fermented feed are on the impact of milking sow production performance
As shown in Table 2, after using compound sugar, 10% fermented feed and 20% fermented feed to feed in lactating swine diets 27-28 days, the feed intake of milking sow improves 3.33%, 9.28% and 9.79% (p < 0.05) than control group respectively, and constipation rate reduces 66.37%, 83.17%, 96.64%.
After using compound sugar, 10% fermented feed and 20% fermented feed to feed in lactating swine diets 27-28 days, the daily gain of piglet improves 6.29%, 22.87% and 25.37% (p < 0.05 or p < 0.01) than control group respectively.
The above results shows: fermented feed can significantly improve the speed of growth of piglet, significantly improves milking sow feed intake, significantly reduces prevention of sow constipation.
Two, novel food calling, the main active substances content in anti-constipation fermented feed prepared of embodiment 1, in table 3.
Main active substances content in the novel food calling of table 3, anti-constipation fermented feed
| Project | Index | Project | Index |
| Color and luster | Yellowish-brown or light yellow | Lactic acid/% | ≥1.5 |
| Moisture/% | ≤12.0 | Crude fibre/% | ≥12.0 |
| Thick protein/% | ≥8.0 | Coarse ash/% | ≤0.4 |
| Crude fat/% | ≥1.2 | Lysine/% | ≥0.30 |
| Little peptide albumen/% | ≥1.5 | Methionine/% | ≥0.10 |
Above content is only citing made for the present invention and explanation; affiliated those skilled in the art make various amendment to described specific embodiment or supplement or adopt similar mode to substitute; only otherwise depart from the design of invention or surmount this scope as defined in the claims, protection scope of the present invention all should be belonged to.
Claims (7)
1. a preparation method for novel food calling, anti-constipation fermented feed, it is characterized in that, step is as follows:
(1), barley, pea, alfalfa meal and molasses, water are mixed modified in proportion, obtained fermentation material wet basis;
(2) in fermentation material wet basis, add saccharomyces cerevisiae bacterial classification fluid strain liquid, Lactobacillus plantarum strain liquid, microbial protease, zytase, dextranase;
(3), solid anaerobic digestion enzymolysis;
(4), obtained novel food calling, anti-constipation fermented feed is dried.
2. the preparation method of novel food calling according to claim 1, anti-constipation fermented feed, it is characterized in that, the weight ratio of water in step (1), barley, pea, alfalfa meal and molasses is 100:1 ~ 300:2 ~ 600:2 ~ 600:2 ~ 600.
3. the preparation method of novel food calling according to claim 2, anti-constipation fermented feed, it is characterized in that, the weight ratio of water in step (1), barley, pea, alfalfa meal and molasses is 100:50 ~ 100:30 ~ 80:30 ~ 80:15 ~ 30.
4. the preparation method of novel food calling according to claim 1, anti-constipation fermented feed, it is characterized in that, in step (2), the weight ratio of fermentation material wet basis, saccharomyces cerevisiae bacterial classification fluid strain liquid, Lactobacillus plantarum strain liquid is 100:0.5 ~ 20:0.5 ~ 20; In step (2), the addition of microbial protease, zytase, dextranase is 0.01-1000U/g, 0.005-500U/g, 0.005-500U/g fermentation material wet basis.
5. the preparation method of novel food calling according to claim 4, anti-constipation fermented feed, it is characterized in that, in step (2), the weight ratio of fermentation material wet basis, saccharomyces cerevisiae bacterial classification fluid strain liquid, Lactobacillus plantarum strain liquid is 100:2 ~ 8:3 ~ 15; In step (2), the addition of microbial protease, zytase, dextranase is 100-300U/g, 50-150U/g, 30-80U/g substrate.
6. the preparation method of novel food calling according to claim 1, anti-constipation fermented feed, is characterized in that, in step (3), fermentation, hydrolysis temperature are 10 ~ 60 DEG C, and fermentation, enzymolysis time are 1 ~ 260h, and the initial pH that ferments is 3-9.
7. the preparation method of novel food calling according to claim 6, anti-constipation fermented feed, is characterized in that, in step (3), fermentation, hydrolysis temperature are 25 ~ 35 DEG C, and fermentation, enzymolysis time are 48 ~ 84h, and the initial pH that ferments is 5.0-6.5.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105876160A (en) * | 2016-04-28 | 2016-08-24 | 安徽五粮泰生物工程股份有限公司 | Method for preparing molasses sugar acidification and glycolysis feed |
| CN105961870A (en) * | 2016-06-15 | 2016-09-28 | 环山集团有限公司 | Batch for improving constipation of sows in lactation period and preparation method thereof |
| CN108497187A (en) * | 2018-03-28 | 2018-09-07 | 周口广安农牧科技有限公司 | A kind of yellow serofluid promotees the preparation method of newborn glycolysis dregs of beans |
| CN112970941A (en) * | 2021-04-19 | 2021-06-18 | 山东新希望六和集团有限公司 | Feeding-attractant glycolysis molasses feed and preparation method thereof |
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| CN1031015A (en) * | 1987-08-24 | 1989-02-15 | 湖南省新化县微生物厂 | Bacterium-containing fermented-feed modifier and preparation thereof |
| CN101288439A (en) * | 2008-06-05 | 2008-10-22 | 赵仲芳 | Production method of straw fermented feed |
| CN104206692A (en) * | 2014-09-19 | 2014-12-17 | 龙岩市益发畜牧发展有限公司 | Preparation method of attractant anti-constipation fermented feed |
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2015
- 2015-12-03 CN CN201510891494.1A patent/CN105410403A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1031015A (en) * | 1987-08-24 | 1989-02-15 | 湖南省新化县微生物厂 | Bacterium-containing fermented-feed modifier and preparation thereof |
| CN101288439A (en) * | 2008-06-05 | 2008-10-22 | 赵仲芳 | Production method of straw fermented feed |
| CN104206692A (en) * | 2014-09-19 | 2014-12-17 | 龙岩市益发畜牧发展有限公司 | Preparation method of attractant anti-constipation fermented feed |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105876160A (en) * | 2016-04-28 | 2016-08-24 | 安徽五粮泰生物工程股份有限公司 | Method for preparing molasses sugar acidification and glycolysis feed |
| CN105961870A (en) * | 2016-06-15 | 2016-09-28 | 环山集团有限公司 | Batch for improving constipation of sows in lactation period and preparation method thereof |
| CN108497187A (en) * | 2018-03-28 | 2018-09-07 | 周口广安农牧科技有限公司 | A kind of yellow serofluid promotees the preparation method of newborn glycolysis dregs of beans |
| CN112970941A (en) * | 2021-04-19 | 2021-06-18 | 山东新希望六和集团有限公司 | Feeding-attractant glycolysis molasses feed and preparation method thereof |
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Address after: 238000 Anhui city of Hefei province Chaohu city Anhui Juchao Wan Road Economic Development Zone No. 18 Applicant after: Hefei Wuliangye Thai Biotechnology Co. Ltd. Address before: 238000 Anhui city of Hefei province Chaohu city Anhui economic open flag Road Juchao Applicant before: HEFEI FLSUGARPEPTIDE BIOLOGY TECHNOLOGY CO., LTD. |
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