CN104686790A - Preparation method of peptidoglycolipid for feed - Google Patents
Preparation method of peptidoglycolipid for feed Download PDFInfo
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Abstract
The invention belongs to the technical field of feeds, enzyme preparations and microorganisms, and in particular relates to a preparation method of peptidoglycolipid for a feed. The preparation method comprises the following steps: liquifying and saccharifying a grain feed through alpha-amylase so as to obtain a grain polysaccharide liquid and a grain solid wet basis; mixing a little grain polysaccharide liquid or clean water, a composite oil for the feed, an emulsifying agent, lipase, an antioxidant and lipovitamin, and performing enzymolysis, emulsification and homogenizing so as to obtain emulsified thick fat liquid; mixing the grain solid wet basis, a protein feed, the clean water, mixed bacterial liquid and protease, and performing fermentation and enzymolysis so as to obtain enzymolysis dreg peptide; adsorbing and mixing two or three kinds of materials in the grain polysaccharide liquid, the emulsified thick fat liquid, the enzymolysis dreg peptide and an adsorbing carrier feed, baking the mixed mixture, and adding an anticaking agent in a mixing manner so as to obtain the peptidoglycolipid for the feed. The peptidoglycolipid for the feed, prepared by the preparation method disclosed by the invention, is rich in emulsified fat, short chain dextrin, glucose, oligosaccharide, small peptide, lactic acid and probiotics, and has the characteristics of being balanced in amino acid, being rich in nutrition, being easy to digest, being good in palatability, being low in the content of anti-nutrition factors and the like.
Description
Technical field
The invention belongs to feed, enzyme preparation and microbial technology field, be specifically related to a kind of preparation method of feeding glycolipidpeptide.
Background technology
China is populous nation, Ye Shi aquaculture big country.2012, the animal husbandry output value reached 22,870 hundred million yuan, and meat, birds, beasts and eggs, milk and output of aquatic products reach 7,925 ten thousand tons, 2,865 ten thousand tons, 3,744 ten thousand tons and 5,373 ten thousand tons respectively.Along with the development of aquaculture, feed industrial development speed is surprising, and within 2012, national commercial feed output reaches 1.94 hundred million tons, wherein, and mixed feed 1.63 hundred million tons, concentrated feed 2,450 ten thousand tons, additive premix 6,190,000 tons.To 12 ends, national mixed feed Straight Run production capacity about reaches 200,000,000 tons, and actual production reaches 1.5 hundred million tons; Concentrated feed output reaches 4,000 ten thousand tons; Additive premix output reaches 8,000,000 tons.According to Ministry of Agriculture's statistics, Chinese pig feed output 7,600 ten thousand tons in 2012, fowl material output 8,733 ten thousand tons, aquatic feeds 1,883 ten thousand tons, 7,800,000 tons, cattle and sheep feed, 3,200,000 tons, the feeds such as rabbit racoon dog pet, wherein, children's animal and fowl fodder in age is up to 2,500 ten thousand tons, and the feed for piglet is more than 6,000,000 tons.
As everyone knows, the exploitation of feed product, the difficult point of development are children's animal and fowl fodder in age, and how improving children's animal and fowl fodder digestibility in age and reduction or reducing ANFs becomes the problem that Animal nutrition scholar in recent years actively inquires into.With regard to children livestock and poultry in age particularly newborn piglet, its metabolism is vigorous, relative growth is rapid, high to the requirement of diet nutrient material, but its gastral function is unsound, gastral weight and volume is less and to send out the region between the heart and the diaphragm incomplete, gastric acid secretion is not enough, gastrointestinal micro-flora is variable, and it is unsound that Digest enzyme sends out the region between the heart and the diaphragm, digestive ferment hyposecretion.Such as, newborn piglet Major Secretory trypsase, pancreatic lipase, lactase are used for the digestion of breast milk, and the activity of 0 ~ 25 age in days lactase is very high, but maltose and invertase secretion deficiency, before 21 ages in days, protease, pancreatic lipase and amylase secretion amount are all not enough; Breast piglet protein matter indigestion, protein easily produces some spoilage product at hindgut, cadaverine, putrescine, histamine etc., stimulates intestinal mucosa, and infringement intestinal villi film, causes intestinal contents osmotic pressure to raise, and enteron aisle dehydration causes diarrhoea.Irritated to vegetable protein, the piglet before 8 week age, causes intestinal mucosa impaired to the easy allergy of vegetable protein, affects the absorption to nutrient, and bacterium is easily invaded and causes diarrhoea.
Undoubtedly, fermented feed palatability, digestibility will obviously improve, but energy often step-down after feed fermentation enzymolysis, how improving fermented feed energy is the difficult problem that must solve.In recent years, newborn piglet lipid nutrition receives much attention, and adipose energy is higher, but the digestibility of newborn piglet to fat such as soybean oil, corn oil, palm oil, rice bran oil and fish oil is very low.Research shows, after fat emulsification, digestibility can increase substantially.Therefore, add chyle fat age in livestock and poultry fermented feed children to be worth inquiring into.
Before this, the preparation method of the granted patent of applicant " a kind of preparation method of children's animal and fowl fodder in age " (patent No.: CN201110421495.1) elaboration livestock and poultry in young age fermentation, enzymolysis feed.But cereal solid content wet basis and fermented bean dregs are not mixed fermentation by this patent, grain polysaccharide liquid is not mixed with grease, fermented bean dregs, emulsification, homogeneous, be not set forth in the method for adding chyle fat in fermentation, enzymolysis feed yet.Meanwhile, this patent adopts sulfuric acid and NaOH to regulate pH, can produce bitter taste, and then affect product palatability, need to use lactic acid, citric acid and calcium hydroxide instead to regulate pH.
Before this, the granted patent of applicant " preparation method of a kind of children livestock and poultry glycolysis in age and emulsification feed " (patent No.: CN201210488914.8), cereal solid content wet basis and fermented bean dregs are successfully mixed fermentation by this patent, success grain polysaccharide liquid is mixed with grease, fermented bean dregs, emulsification, homogeneous, and success with the addition of chyle fat in fermentation, enzymolysis feed, successfully adopts lactic acid, citric acid and calcium hydroxide to regulate pH.But this patent selects to there is certain deficiency in technique and raw material, has occurred many problems in the large production of actual industrialization more than a year.
Now according to the industrialization knowhow of applicant, perfect below having done:
1., due to cellulose can slow down sow and piglet constipation, therefore this patent adds the starch-containing matter such as paddy, barley, oat, Ipomoea batatas and the technical matters that the feed that enriches of content of cellulose and dilated product thereof carry out enzymolysis is set forth.
2., because high, the cotton dish dregs of rice of beans fat content are cheap, animal protein good feeding effect, albumen powder are conducive to amino acid balance, therefore this patent adds protein feeds and the dilated products thereof such as soybean, pea, red bean, cotton dregs, rapeseed dregs, fish meal, blood meal, plasma proteins, wheat gluten flour, rice protein powder, corn protein powder, DDGS, combine the technical matters of carrying out fermenting after even with cereal solid content wet basis mixture and set forth.
3., because feeding effect needs and absorption oven dry needs, therefore this patent adds absorption carrier feed by syrup, after breast solution fat slurry directly adsorbs, the technical matters of drying is set forth, described absorption carrier feed is corn, paddy, crack rice or rice, wheat, wheat bran, rice bran, flour, oat, barley, Ipomoea batatas, soybean, dregs of beans, pea, red bean, cotton dregs, rapeseed dregs, fish meal, blood meal, plasma proteins, hyperglobulinemia, lactose, whey powder, DDDS, corn protein powder, rice protein powder, wheat gluten flour, soybean protein isolate, FSPC, fermentation grouts (as fermented bean dregs), starch, maltodextrin, one or two or more kinds combination product of the feedstuff such as pasture powder and Chinese herbal medicine and dilated product thereof.
4., due to glycolysis slag dregs of rice peptide homogeneous, emulsification get up often blocking, practical operation is very difficult, therefore this patent does not re-use glycolysis slag dregs of rice peptide in grease homogeneous, emulsification in producing.
5., owing to not re-using glycolysis slag dregs of rice peptide in fat emulsification, homogenization, therefore this patent no longer needs grinder to grind at fat emulsification, homogeneous in producing.
6., because fat does not have enzymolysis, lipochondrion is still bigger than normal, and digestibility is still on the low side, therefore this patent interpolation uses lipase to carry out enzymolysis, generates Small molecular aliphatic acid.
7., due to fatty oil vitamin, particularly vitamin E, can be anti-oxidant, particularly can improve breeder performance, therefore this patent adds use liposoluble vitamin.
8., due to the easy moisture absorption caking of product, had a strong impact on product promotion use, therefore this patent adds anti-caking agent adding technology, as tricalcium phosphate, white carbon etc.
9., due to grease easy oxidative rancidity in summer, therefore this patent adds antioxidant adding technology.
Summary of the invention
The present invention is intended to overcome that above-mentioned children's its gastral function of livestock and poultry in age is unsound, gastric acid secretion is not enough, gastrointestinal micro-flora is variable, digestive ferment hyposecretion, the protein digestibility bad intestinal contents osmotic pressure that easily causes raises, and enteron aisle dewaters and causes the difficult problems such as diarrhoea.There is provided one to be rich in chyle fat, short chain dextrin, glucose, compound sugar, little peptide, lactic acid and probio, have that nutrition is high, easy to digest, good palatability and the low feeding glycolipidpeptide of ANFs content.
The technical solution adopted in the present invention is: a kind of preparation method of feeding glycolipidpeptide, and step is as follows:
1), by the grain trough containing starch through α-amylaseliquefied, saccharification, Separation of Solid and Liquid obtains grain polysaccharide liquid and cereal solid content wet basis;
2), by enzymolysis, emulsification, homogeneous after a small amount of grain polysaccharide liquid or the mixing of clean water, compound feeding oil, emulsifying agent, lipase, antioxidant and liposoluble vitamin, obtained breast separates fat slurry;
3), by the mixing of cereal solid content wet basis, protein feeds, clean water, mixed bacteria liquid and protease, fermentation, enzymolysis, obtained glycolysis slag dregs of rice peptide;
4), the preparation of feeding glycolipidpeptide
By grain polysaccharide liquid full dose prepared by grain polysaccharide liquid (when adopting water preparation breast to separate fat slurry, grain polysaccharide liquid is herein then step 1).When adopting a small amount of grain polysaccharide liquid preparation breast to separate fat slurry, grain polysaccharide liquid is herein then step 1) surplus of grain polysaccharide liquid prepared.Below illustrate no longer separately), breast separates fat slurry, two or three absorption, mixing in glycolysis slag dregs of rice peptide, absorption carrier feed, dry after, then be mixed into anti-caking agent and obtain feeding glycolipidpeptide (ferment, enzymolysis and emulsification feed);
Or;
Grain polysaccharide liquid and breast separated fat slurry adsorbs separately with glycolysis slag dregs of rice peptide, mixes, after drying, then be mixed into anti-caking agent and obtain feeding glycolipidpeptide;
Or;
By grain polysaccharide liquid, breast separates fat slurry, glycolysis slag dregs of rice peptide adsorbs, mixes together with absorption carrier feed, after drying, then be mixed into anti-caking agent and obtain feeding glycolipidpeptide;
Or;
Grain polysaccharide liquid and breast are separated fat starch drying respectively and make Icing Sugar and cosmetics, glycolysis slag dregs of rice Gly-His-Lys is made in the drying of glycolysis slag dregs of rice peptide, then glycolysis slag dregs of rice Gly-His-Lys, Icing Sugar and cosmetics are mixed, then be mixed into anti-caking agent and obtain feeding glycolipidpeptide;
Or;
After grain polysaccharide liquid and breast being separated the mixing of fat slurry, glycolipid powder is made in drying, and glycolysis slag dregs of rice Gly-His-Lys is made in the drying of glycolysis slag dregs of rice peptide, then by after glycolysis slag dregs of rice Gly-His-Lys and the mixing of glycolipid powder, then is mixed into anti-caking agent and obtains feeding glycolipidpeptide.
Preferably, step 1) in the preparation process of grain polysaccharide liquid and cereal solid content wet basis as follows:
1. the grain trough impurity elimination, by containing starch, pulverizing, be 100: 100 ~ 10000 add clean water by the weight ratio of grain trough and water, be modulated into enzymolysis slurries, be warming up to 50 ~ 150 DEG C of liquefaction, with 20% aqua calcis, pH be adjusted to 2.0 ~ 12.0;
Grain trough containing starch is corn, wheat, crack rice or the dilated product of rice, paddy, flour, oat, barley and Ipomoea batatas or each cereal, and weight ratio is 100: 0 ~ 300: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 500.Certainly, food or feed that other kind contains starch can also be comprised.
2., by 0.1 ~ 100U/g substrate butt dosage, AMS is added, liquefaction enzymolysis 0.05 ~ 24h;
3., be cooled to 30 ~ 90 DEG C, with 80% lactic acid solution or citric acid solution, pH be adjusted to 1.0 ~ 8.0;
4., carbohydrase is added by 0.05 ~ 10000U/g substrate butt dosage, zytase is added by 0.05 ~ 1200U/g substrate butt dosage, dextranase is added by 0.05 ~ 1200U/g substrate butt dosage, after enzymatic saccharification 0.05 ~ 96h, isolate cereal solid content wet basis and grain polysaccharide liquid through solid-liquid separating equipment.
Further preferred, step corn 1., wheat, crack rice or rice, paddy, flour, oat, barley and Ipomoea batatas weight ratio be 100: 0 ~ 30: 0 ~ 50: 0 ~ 50: 0 ~ 50: 0 ~ 50: 0 ~ 50: 0 ~ 50;
Step condensing temperature is 1. 65 ~ 105 DEG C, and liquefaction enzymolysis pH is 5.8 ~ 6.6;
Step AMS addition is 2. 8.0 ~ 12U/g substrate butt, and liquefaction enzymolysis time is 2.0 ~ 4h;
Step 3. be cooled to 60 ~ 70 DEG C, pH is adjusted to 4.0 ~ 5.0;
The step enzymatic saccharification time is 4. 2 ~ 48h.
Preferably, step 2) in compound feeding oil be soybean oil, corn oil, palm oil, rice bran oil and fish oil, by a small amount of grain polysaccharide liquid or clean water, soybean oil, corn oil, palm oil, rice bran oil, fish oil, emulsifying agent, lipase, antioxidant and liposoluble vitamin are 100: 0 ~ 600: 0 ~ 600: 0 ~ 600: 0 ~ 600: 0 ~ 600: 0.005 ~ 50: 0.00005 ~ 5.0: 0.00001 ~ 0.5: 0.005 ~ 50 to mix by weight, make fatty sugared slag liquid, enzymolysis is carried out after being filtered by sugared for fat slag liquid, emulsification, homogeneous, make breast and separate fat slurry.
Preferred further, a small amount of grain polysaccharide liquid or clean water, soybean oil, corn oil, palm oil, rice bran oil, fish oil, emulsifying agent, lipase, antioxidant and liposoluble vitamin are 100: 20 ~ 500: 20 ~ 200: 10 ~ 100: 10 ~ 100: 10 ~ 100: 0.5 ~ 10: 0.001 ~ 2.0: 0.0001 ~ 0.1: 0.05 ~ 5 by weight.
Preferably, step 3) in the preparation process of glycolysis slag dregs of rice peptide as follows:
A, by after protein feeds impurity elimination, pulverizing, be 100: 0 ~ 3000: 0 ~ 6000: 0.01 ~ 100 mixing by the weight ratio of cereal solid content wet basis, protein feeds, clean water and mixed bacteria liquid, puddle evenly and be warmed up to 15 ~ 50 DEG C;
Protein feeds is the dilated product of dregs of beans, soybean, pea, red bean, cotton dregs, rapeseed dregs, fish meal, blood meal, plasma proteins, wheat gluten flour, corn protein powder, rice protein powder, DDGS or each feed, and weight ratio is 100: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 300: 0 ~ 300: 0 ~ 300: 0 ~ 300: 0 ~ 300: 0 ~ 300: 0 ~ 300.Certainly, other grouts or protein feeds can also be comprised.
Yeast seeds liquid in mixed bacteria liquid, lactic acid bacteria culturers liquid, Bacillus subtilis strain liquid containing spore number of viable ratio be 100: 0 ~ 800: 0 ~ 800;
B, by 0.005 ~ 2000U/g substrate wet basis dosage, add microbial protease;
C, mix after fermentation and enzymolysis 8 ~ 132h, make glycolysis slag dregs of rice peptide.
Further preferred, the weight ratio of the dregs of beans of steps A, soybean, pea, red bean, cotton dregs, rapeseed dregs, fish meal, blood meal, plasma proteins, wheat gluten flour, corn protein powder, rice protein powder and DDGS is 100: 0 ~ 30: 0 ~ 50: 0 ~ 50: 0 ~ 50: 0 ~ 50: 0 ~ 30: 0 ~ 30: 0 ~ 30: 0 ~ 30: 0 ~ 50: 0 ~ 50: 0 ~ 50;
The weight ratio of the cereal solid content wet basis of steps A, protein feeds, clean water and mixed bacteria liquid is 100: 0 ~ 500: 0 ~ 1000: 5 ~ 20 mixing, puddles evenly and is warmed up to 28 ~ 35 DEG C;
The yeast seeds liquid of steps A, lactic acid bacteria culturers liquid, Bacillus subtilis strain liquid be 100: 200 ~ 300: 50 ~ 100 containing spore number of viable ratio;
The microbial protease additive capacity of step B is 60.0 ~ 120U/g substrate wet basis;
The fermentation of step C and enzymolysis time are 36 ~ 84h.
Preferably, step 4) in absorption carrier feed be corn, paddy, crack rice or rice, wheat, wheat bran, rice bran, flour, oat, barley, Ipomoea batatas, soybean, dregs of beans, pea, red bean, cotton dregs, rapeseed dregs, fish meal, blood meal, plasma proteins, hyperglobulinemia, lactose, whey powder, DDDS, corn protein powder, rice protein powder, wheat gluten flour, soybean protein isolate, FSPC, fermentation grouts, starch, maltodextrin, pasture powder and Chinese herbal medicine and each raw material thereof dilated product in one or more mixture.
Feeding glycolipidpeptide (fermentation, enzymolysis and emulsification feed prepared by the present invention, trade name five grain peptide), be rich in chyle fat, short chain dextrin, glucose, compound sugar, little peptide, lactic acid and probio, have that amino acid balance, nutrition are high, easy to digest, good palatability and the feature such as ANFs content is low.
Detailed description of the invention
Below with reference to embodiment, the present invention is described in detail.
Embodiment 1
By 600kg corn, 400kg cracks rice or rice impurity elimination is pulverized, mix, add 1600kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, by 20% aqua calcis (mass percent, lower same) pH is adjusted to 6.0 ~ 6.2, by adding 2kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, by 20% lactic acid solution (mass percent, lower same) pH is adjusted to 4.5 ~ 4.6, add 2kg carbohydrase (2U/g substrate butt), 1kg zytase (10U/g substrate butt), 0.5kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 2000 ~ 2100kg grain polysaccharide liquid and 500 ~ 600kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 100kg clean water, 500 ~ 600kg cereal solid content (wet basis) and 100kg dregs of beans, the impurity elimination of 100kg soybean are pulverized, access 120kg mixed bacteria liquid (all prepared by prior art by each strain liquid, lower same), add microbial protease 0.5kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 800 ~ 900kg glycolysis slag dregs of rice peptide (wet basis).
After being mixed with 120kg grain polysaccharide liquid, 0.2kg emulsifying agent, 0.1kg lipase, 0.01kg liquid antioxidant, 0.12kg liposoluble vitamin by 60kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil), separate fat slurry via making 180kg breast after mixing, enzymolysis, emulsification, homogeneous.
After 1880 ~ 1980kg grain polysaccharide liquid, 800 ~ 900kg glycolysis slag dregs of rice peptide, 180kg breast are separated fat slurry three mixing, convection drying, after being mixed into anti-caking agent 15kg, make feeding glycolipidpeptide.
Embodiment 2
By 700kg corn, 300kg wheat (or flour) impurity elimination is pulverized, mix, add 1600kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 2kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, with 20% lactic acid solution, pH is adjusted to 4.5 ~ 4.6, add 2kg carbohydrase (2U/g substrate butt), 1kg zytase (10U/g substrate butt), 0.5kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 2000 ~ 2100kg grain polysaccharide liquid and 500 ~ 600kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 20kg clean water, 500 ~ 600kg cereal solid content (wet basis) and 80kg dregs of beans, 80kg soybean and the impurity elimination of 20kg pea are pulverized, access 80kg mixed bacteria liquid, add microbial protease 0.5kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 780 ~ 880kg glycolysis slag dregs of rice peptide (wet basis).
After being mixed with 120kg water, 0.2kg emulsifying agent, 0.3kg lipase, 0.01kg liquid antioxidant, 0.20kg liposoluble vitamin by 60kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil), separate fat slurry via making 180kg breast after mixing, enzymolysis, emulsification, homogeneous.
After 2000 ~ 2100kg grain polysaccharide liquid, 180kg breast are separated the mixing of fat slurry, glycolipid powder is made after first drying, simultaneously by after the drying of 780 ~ 880kg glycolysis slag dregs of rice peptide, be mixed into anti-caking agent 12kg and make glycolysis slag dregs of rice Gly-His-Lys, then make feeding glycolipidpeptide after three being mixed.
Embodiment 3
The impurity elimination of 1000kg corn is pulverized, mix, add 1600kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 2kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, with 20% lactic acid solution, pH is adjusted to 4.5 ~ 4.6, add 2kg carbohydrase (2U/g substrate butt), 1kg zytase (10U/g substrate butt), 0.5kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 2000 ~ 2100kg grain polysaccharide liquid and 500 ~ 600kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 60kg clean water, 500 ~ 600kg cereal solid content (wet basis) and 120kg dregs of beans, the impurity elimination of 60kg soybean are pulverized, access 100kg mixed bacteria liquid, add microbial protease 0.5kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 780 ~ 880kg glycolysis slag dregs of rice peptide (wet basis).
After being mixed with 50kg grain polysaccharide liquid, 0.2kg emulsifying agent, 0.1kg lipase, 0.01kg liquid antioxidant, 0.15kg liposoluble vitamin by 60kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil), separate fat slurry via making 160kg breast after mixing, enzymolysis, emulsification, homogeneous.
After 1950 ~ 2050kg grain polysaccharide liquid, 160kg breast are separated the mixing of fat slurry, make glycolipid powder after first drying, make glycolysis slag dregs of rice Gly-His-Lys by after the drying of 780 ~ 880kg glycolysis slag dregs of rice peptide simultaneously, then make feeding glycolipidpeptide after mixing with 12kg anti-caking agent.
Embodiment 4
By 600kg corn, 200kg wheat, 200kg cracks rice or rice impurity elimination is pulverized, mix, add 1500kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 2kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, with 20% lactic acid solution, pH is adjusted to 4.5 ~ 4.6, add 2kg carbohydrase (2U/g substrate butt), 1kg zytase (10U/g substrate butt), 0.5kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 1900 ~ 2000kg grain polysaccharide liquid and 500 ~ 600kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 500 ~ 600kg cereal solid content (wet basis) and 100kg dregs of beans, the impurity elimination of 80kg cotton dregs are pulverized, access 120kg mixed bacteria liquid, add microbial protease 0.5kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 780 ~ 880kg glycolysis slag dregs of rice peptide (wet basis).
After being mixed with 50kg water, 0.2kg emulsifying agent, 0.2kg lipase, 0.01kg liquid antioxidant, 0.18kg liposoluble vitamin by 60kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil), separate fat slurry via making 140kg breast after mixing, enzymolysis, emulsification, homogeneous.
After 1850 ~ 1950kg grain polysaccharide liquid, 140kg breast solution fat slurry and 780 ~ 880kg glycolysis slag dregs of rice peptide (wet basis) three are mixed, directly dry, then make feeding glycolipidpeptide after mixing with 10kg anti-caking agent.
Embodiment 5
By 500kg corn, the impurity elimination of 500kg oat is pulverized, mix, add 1600kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 2kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, with 20% lactic acid solution, pH is adjusted to 4.5 ~ 4.6, add 2kg carbohydrase (2U/g substrate butt), 1kg zytase (10U/g substrate butt), 0.5kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 2000 ~ 2100kg grain polysaccharide liquid and 500 ~ 600kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 50kg clean water, 500 ~ 600kg cereal solid content (wet basis) and the impurity elimination of 180kg dregs of beans are pulverized, access 100kg mixed bacteria liquid, add microbial protease 0.5kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 780 ~ 880kg glycolysis slag dregs of rice peptide (wet basis).
After being mixed with 800kg water, 1.2kg emulsifying agent, 0.9kg lipase, 0.06kg liquid antioxidant, 0.50kg liposoluble vitamin by 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil), separate fat slurry via making 1100kg breast after mixing, enzymolysis, emulsification, homogeneous.
1200 ~ 1300kg grain polysaccharide liquid, 780 ~ 880kg glycolysis slag dregs of rice peptide (wet basis), 1100kg breast are separated fat slurry three and mixed rear, convection drying, then make feeding glycolipidpeptide after mixing with 20kg anti-caking agent.
Embodiment 6
By 600kg corn, 400kg wheat (or secondary powder) impurity elimination is pulverized, mix, add 1600kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 2kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, with 20% lactic acid solution, pH is adjusted to 4.5 ~ 4.6, add 2kg carbohydrase (2U/g substrate butt), 1kg zytase (10U/g substrate butt), 0.5kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 2000 ~ 2100kg grain polysaccharide liquid and 500 ~ 600kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 500kg clean water, 500 ~ 600kg cereal solid content (wet basis) and 100kg soybean, the impurity elimination of 80kg rapeseed dregs are pulverized, access 100kg mixed bacteria liquid, add microbial protease 0.5kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 780 ~ 880kg glycolysis slag dregs of rice peptide (wet basis).
After being mixed with 800kg grain polysaccharide liquid, 1.2kg emulsifying agent, 1.0kg lipase, 0.06kg liquid antioxidant, 0.6kg liposoluble vitamin by 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil), separate fat slurry via making 1100kg breast after mixing, enzymolysis, emulsification, homogeneous.
After 1200 ~ 1300kg grain polysaccharide liquid, 1100kg breast are separated the mixing of fat slurry, make glycolipid powder after first drying, make glycolysis slag dregs of rice Gly-His-Lys by after the drying of 780 ~ 880kg glycolysis slag dregs of rice peptide simultaneously, then make feeding glycolipidpeptide after mixing with 15kg anti-caking agent.
Embodiment 7
The impurity elimination of 1000kg wheat is pulverized, mix, add 1600kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 2kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, with 20% lactic acid solution, pH is adjusted to 4.5 ~ 4.6, add 2kg carbohydrase (2U/g substrate butt), 1kg zytase (10U/g substrate butt), 0.5kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 2000 ~ 2100kg grain polysaccharide liquid and 500 ~ 600kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 300kg clean water, 500 ~ 600kg cereal solid content (wet basis) and 100kg dregs of beans, the impurity elimination of 80kg pea are pulverized, access 100kg mixed bacteria liquid, add microbial protease 0.5kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 780 ~ 880kg glycolysis slag dregs of rice peptide (wet basis).
By 2000 ~ 2100kg grain polysaccharide liquid and 780 ~ 880kg glycolysis slag dregs of rice peptide (wet basis) mixing, directly dry, then after mixing with 20kg anti-caking agent, make children's livestock and poultry glycolysis in age feed.
Embodiment 8
By 800kg corn, the impurity elimination of 200kg Ipomoea batatas is pulverized, mix, add 1600kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 2kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, with 20% lactic acid solution, pH is adjusted to 4.5 ~ 4.6, add 2kg carbohydrase (2U/g substrate butt), 1kg zytase (10U/g substrate butt), 0.5kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 2000 ~ 2100kg grain polysaccharide liquid and 500 ~ 600kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 600kg clean water, 500 ~ 600kg cereal solid content (wet basis) and 100kg dregs of beans, the impurity elimination of 80kg soybean are pulverized, access 100kg mixed bacteria liquid, add microbial protease 0.5kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 780 ~ 880kg glycolysis slag dregs of rice peptide (wet basis).
Make Icing Sugar by after first for 2000 ~ 2100kg grain polysaccharide liquid drying, make glycolysis slag dregs of rice Gly-His-Lys by after the drying of 780 ~ 880kg glycolysis slag dregs of rice peptide simultaneously, then make feeding glycolipidpeptide after mixing after the two being mixed with 10kg anti-caking agent.
Embodiment 9
By 100kg corn, the impurity elimination of 100kg flour is pulverized, mix, add 300kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 0.5kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, by 20% citric acid solution (mass percent, lower same) pH is adjusted to 4.5 ~ 4.6, add 0.4kg carbohydrase (2U/g substrate butt), 0.2kg zytase (10U/g substrate butt), 0.1kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 360 ~ 400kg grain polysaccharide liquid and 110 ~ 120kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 60kg clean water, 110 ~ 120kg cereal solid content (wet basis) and the impurity elimination of 30kg dregs of beans are pulverized, access 15kg mixed bacteria liquid, add microbial protease 0.1kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 155 ~ 165kg glycolysis slag dregs of rice peptide (wet basis).
By 200 ~ 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil) with, 0.6kg emulsifying agent, 0.8kg lipase, 0.05kg liquid antioxidant, 0.50kg liposoluble vitamin mix, make 355 ~ 395kg breast after adding suitable quantity of water mixing, enzymolysis, emulsification, homogeneous and separate fat slurry.
After 355 ~ 395kg breast being separated fat slurry and the mixing of 360 ~ 400kg grain polysaccharide liquid, directly dry, then make feeding glycolipidpeptide after mixing with 3kg anti-caking agent.
Embodiment 10
By 100kg corn, 80kg cracks rice or rice impurity elimination is pulverized, mix, add 300kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 0.4kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, with 20% citric acid solution, pH is adjusted to 4.5 ~ 4.6, add 0.4kg carbohydrase (2U/g substrate butt), 0.2kg zytase (10U/g substrate butt), 0.1kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 360 ~ 400kg grain polysaccharide liquid and 110 ~ 120kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 120kg clean water, 110 ~ 120kg cereal solid content (wet basis) and the impurity elimination of 30kg dregs of beans are pulverized, access 15kg mixed bacteria liquid, add microbial protease 0.1kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 155 ~ 165kg glycolysis slag dregs of rice peptide (wet basis).
After 200 ~ 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil) is mixed with 0.6kg emulsifying agent, 0.8kg lipase, 0.05kg liquid antioxidant, 0.50kg liposoluble vitamin, make 355 ~ 465kg breast after adding suitable quantity of water mixing, enzymolysis, emulsification, homogeneous and separate fat slurry.
Make fat dregs of rice powder after 355 ~ 465kg breast being separated the first drying of fat slurry, make polysaccharide powder by after the drying of 360 ~ 400kg grain polysaccharide liquid simultaneously, then make feeding glycolipidpeptide after the two being mixed with 2kg anti-caking agent.
Embodiment 11
By 100kg corn, the impurity elimination of 100kg oat is pulverized, mix, add 300kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 0.4kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, with 20% citric acid solution, pH is adjusted to 4.5 ~ 4.6, add 0.4kg carbohydrase (2U/g substrate butt), 0.2kg zytase (10U/g substrate butt), 0.1kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 12 ~ 24h, 350 ~ 390kg grain polysaccharide liquid and 120 ~ 130kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 120 ~ 130kg cereal solid content (wet basis) and 20kg soybean, the impurity elimination of 20kg cotton dregs are pulverized, access 15kg mixed bacteria liquid, add microbial protease 0.1kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 175 ~ 185kg glycolysis slag dregs of rice peptide (wet basis).
After 200 ~ 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil) is mixed with 0.6kg emulsifying agent, 0.8kg lipase, 0.05kg liquid antioxidant, 0.60kg liposoluble vitamin, make 375 ~ 485kg breast after adding suitable quantity of water mixing, enzymolysis, emulsification, homogeneous and separate fat slurry.
Make fat dregs of rice powder after 375 ~ 485kg breast being separated the first drying of fat slurry, make polysaccharide powder by after the drying of 350 ~ 390kg grain polysaccharide liquid simultaneously, then make feeding glycolipidpeptide after the two being mixed with 3kg anti-caking agent.
Embodiment 12
By 100kg barley, 100kg cracks rice or rice impurity elimination is pulverized, mix, add 300kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 0.5kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, by 20% citric acid solution (mass percent, lower same) pH is adjusted to 4.5 ~ 4.6, add 0.4kg carbohydrase (2U/g substrate butt), 0.2kg zytase (10U/g substrate butt), 0.1kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 360 ~ 380kg grain polysaccharide liquid and 130 ~ 140kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 100kg clean water, 130 ~ 140kg cereal solid content (wet basis) and 30kg pea, the impurity elimination of 20kg soybean are pulverized, access 20kg mixed bacteria liquid, add microbial protease 0.1kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 170 ~ 180kg glycolysis slag dregs of rice peptide (wet basis).
200 ~ 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil) is mixed with 0.6kg emulsifying agent, 0.8kg lipase, 0.05kg liquid antioxidant, 0.6kg liposoluble vitamin, makes 370 ~ 410kg breast after adding suitable quantity of water mixing, enzymolysis, emulsification, homogeneous and separate fat slurry.
After 370 ~ 410kg breast being separated fat slurry and the mixing of 360 ~ 380kg grain polysaccharide liquid, directly dry, then make feeding glycolipidpeptide after mixing with 1.5kg anti-caking agent.
Embodiment 13
The impurity elimination of 1000kg corn is pulverized, mix, add 300kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 0.5kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, by 20% citric acid solution (mass percent, lower same) pH is adjusted to 4.5 ~ 4.6, add 0.4kg carbohydrase (2U/g substrate butt), 0.2kg zytase (10U/g substrate butt), 0.1kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 360 ~ 380kg grain polysaccharide liquid and 130 ~ 140kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
By 200kg clean water, 130 ~ 140kg cereal solid content (wet basis) and 50kg dregs of beans (impurity elimination is pulverized rear), access 20kg mixed bacteria liquid, add microbial protease 0.1kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 170 ~ 180kg glycolysis slag dregs of rice peptide (wet basis).
By 200 ~ 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil), 0.6kg emulsifying agent, 0.8kg lipase, the mixing of 0.15kg liposoluble vitamin, make 200 ~ 300kg breast after adding suitable quantity of water mixing, enzymolysis, emulsification, homogeneous and separate fat slurry.
After 200 ~ 300kg breast is separated fat slurry, 360 ~ 380kg grain polysaccharide liquid, 170 ~ 180kg glycolysis slag dregs of rice peptide and 500kg popcorn mixing and absorption, directly dry, then make feeding glycolipidpeptide after mixing with 15kg anti-caking agent.
Embodiment 14
By 500kg corn, the impurity elimination of 500kg wheat is pulverized, mix, add 350kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 0.5kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, by 20% citric acid solution (mass percent, lower same) pH is adjusted to 4.5 ~ 4.6, add 0.4kg carbohydrase (2U/g substrate butt), 0.2kg zytase (10U/g substrate butt), 0.1kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 360 ~ 380kg grain polysaccharide liquid and 130 ~ 140kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
By 500kg clean water, 130 ~ 140kg cereal solid content (wet basis) and 50kg dregs of beans (impurity elimination is pulverized rear), access 20kg mixed bacteria liquid, add microbial protease 0.1kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 170 ~ 180kg glycolysis slag dregs of rice peptide (wet basis).
By 200 ~ 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil), 0.8kg emulsifying agent, 0.8kg lipase, the mixing of 0.20kg liposoluble vitamin, make 200 ~ 300kg breast after adding suitable quantity of water mixing, enzymolysis, emulsification, homogeneous and separate fat slurry.
After 200 ~ 300kg breast is separated fat slurry, 360 ~ 380kg grain polysaccharide liquid, 170 ~ 180kg glycolysis slag dregs of rice peptide and 500kg swelling soya dreg mixing and absorption, directly dry, then make feeding glycolipidpeptide after mixing with 15kg anti-caking agent.
Embodiment 15
By 100kg corn, 100kg cracks rice or rice impurity elimination is pulverized, mix, add 400kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 0.5kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, by 20% citric acid solution (mass percent, lower same) pH is adjusted to 4.5 ~ 4.6, add 0.4kg carbohydrase (2U/g substrate butt), 0.2kg zytase (10U/g substrate butt), 0.1kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 360 ~ 400kg grain polysaccharide liquid and 110 ~ 120kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
After 110 ~ 120kg cereal solid content (wet basis) and the impurity elimination of 30kg dregs of beans are pulverized, access 15kg mixed bacteria liquid, add microbial protease 0.1kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 155 ~ 165kg glycolysis slag dregs of rice peptide (wet basis).
By 200 ~ 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil) with, 0.6kg emulsifying agent, 0.8kg lipase, 0.05kg liquid antioxidant, 0.50kg liposoluble vitamin mix, make 355 ~ 395kg breast after adding suitable quantity of water mixing, enzymolysis, emulsification, homogeneous and separate fat slurry.
After 355 ~ 395kg breast being separated fat slurry, 360 ~ 400kg grain polysaccharide liquid, 155 ~ 165kg glycolysis slag dregs of rice peptide (wet basis) and 600kg popcorn absorption mixing, directly dry, then make feeding glycolipidpeptide after mixing with 5kg anti-caking agent.
Embodiment 16
By 500kg corn, the impurity elimination of 500kg wheat is pulverized, mix, add 350kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 0.5kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, by 20% citric acid solution (mass percent, lower same) pH is adjusted to 4.5 ~ 4.6, add 0.4kg carbohydrase (2U/g substrate butt), 0.2kg zytase (10U/g substrate butt), 0.1kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 360 ~ 380kg grain polysaccharide liquid and 130 ~ 140kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
130 ~ 140kg cereal solid content (wet basis) is sold.
By 200 ~ 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil), 0.8kg emulsifying agent, 0.8kg lipase, the mixing of 0.20kg liposoluble vitamin, make 200 ~ 300kg breast after adding suitable quantity of water mixing, enzymolysis, emulsification, homogeneous and separate fat slurry.
After 200 ~ 300kg breast is separated fat slurry, 360 ~ 380kg grain polysaccharide liquid and 500kg swelling soya dreg mixing and absorption, directly dry, then make feeding glycolipidpeptide after mixing with 15kg anti-caking agent.
Embodiment 17
By 100kg corn, 100kg cracks rice or rice impurity elimination is pulverized, mix, add 400kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 0.5kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, by 20% citric acid solution (mass percent, lower same) pH is adjusted to 4.5 ~ 4.6, add 0.4kg carbohydrase (2U/g substrate butt), 0.2kg zytase (10U/g substrate butt), 0.1kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 360 ~ 400kg grain polysaccharide liquid and 110 ~ 120kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
110 ~ 120kg cereal solid content (wet basis) is sold.
By 200 ~ 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil) with, 0.6kg emulsifying agent, 0.8kg lipase, 0.05kg liquid antioxidant, 0.50kg liposoluble vitamin mix, make 355 ~ 395kg breast after adding suitable quantity of water mixing, enzymolysis, emulsification, homogeneous and separate fat slurry.
After 355 ~ 395kg breast being separated fat slurry, 360 ~ 400kg grain polysaccharide liquid and 600kg popcorn absorption mixing, directly dry, then make feeding glycolipidpeptide after mixing with 5kg anti-caking agent.
Embodiment 18
By 800kg corn, the impurity elimination of 200kg flour is pulverized, mix, add 350kg water again, be modulated into enzymolysis slurries, be warming up to 90 ~ 95 DEG C of liquefaction, with 20% aqua calcis, pH is adjusted to 6.0 ~ 6.2, by adding 0.5kg AMS (0.5 ~ 25U/g substrate butt), enzymolysis 1 ~ 1.5h, by 20% citric acid solution (mass percent, lower same) pH is adjusted to 4.5 ~ 4.6, add 0.4kg carbohydrase (2U/g substrate butt), 0.2kg zytase (10U/g substrate butt), 0.1kg dextranase (0.5U/g substrate butt), under temperature is 40 DEG C of conditions after enzymatic saccharification 24 ~ 36h, 360 ~ 380kg grain polysaccharide liquid and 130 ~ 140kg cereal solid content (wet basis) is isolated via solid-liquid separating equipment.
By 150kg clean water, 100 ~ 110kg cereal solid content (wet basis) and 70kg dregs of beans (impurity elimination is pulverized rear), access 20kg mixed bacteria liquid, add microbial protease 0.1kg (500U/g substrate butt), puddle evenly and be warmed up to 38 ~ 40 DEG C, after mixing after fermentation and enzymolysis 36h, make 170 ~ 180kg glycolysis slag dregs of rice peptide (wet basis).
By 200 ~ 300kg compound feeding oil (soybean oil, corn oil, palm oil, rice bran oil and fish oil), 0.8kg emulsifying agent, 0.8kg lipase, the mixing of 0.20kg liposoluble vitamin, make 200 ~ 300kg breast after adding suitable quantity of water mixing, enzymolysis, emulsification, homogeneous and separate fat slurry.
After 200 ~ 300kg breast is separated fat slurry, 360 ~ 380kg grain polysaccharide liquid, 170 ~ 180kg glycolysis slag dregs of rice peptide and 500kg corn protein powder mixing and absorption, directly dry, then make feeding glycolipidpeptide after mixing with 15kg anti-caking agent.
One, the feeding glycolipidpeptide Middle nutrition content of material prepared of embodiment 1-4, as shown in table 1.
Feeding glycolipidpeptide Middle nutrition content of material prepared by table 1 embodiment 1-4
Two, in the feeding glycolipidpeptide that prepared by embodiment 1-4, main solvable active material 7 measured values, as shown in table 2.
Main solvable active material 7 measured values in feeding glycolipidpeptide prepared by table 2 embodiment 1-4
| Numbering | Crude fat | Short chain dextrin | Glucose | Lactic acid (mg/g) | The heavy albumen of acid | Probio quantity |
| (mg／g) | (mg／g) | (mg／g) | (mg／g) | (CFU／g) | ||
| 1 | 109.77 | 62.21 | 356.20 | 22.70 | 22.98 | 0.36×10 7 |
| 2 | 112.23 | 59.98 | 365.17 | 30.71 | 24.56 | 0.31×10 7 |
| 3 | 107.17 | 60.19 | 378.28 | 29.55 | 28.87 | 0.33×10 7 |
| 4 | 106.24 | 67.24 | 371.96 | 28.77 | 30.09 | 0.35×10 7 |
| 5 | 111.22 | 59.90 | 383.50 | 30.19 | 29.78 | 0.32×10 7 |
| 6 | 123.01 | 58.86 | 370.91 | 29.79 | 30.81 | 0.36×10 7 |
| 7 | 107.91 | 60.12 | 377.42 | 27.80 | 29.82 | 0.34×10 7 |
| On average | 111.08 | 61.21 | 371.92 | 28.50 | 28.13 | 0.34×10 7 |
Note: probio quantity is containing Bacillus globigii spores number.
Three, the feeding effect of the feeding glycolipidpeptide of embodiment 1 preparation
Choose 28 age in days weanling pig 300 on Agricultural University Of Anhui's test pig farm, close by body weight, parity, the principle of sex half and half is divided into 5 groups at random, often organizes 6 repetitions, each repetition 10 pigs.Feeding glycolipidpeptide (fermentation, enzymolysis and emulsification feed, hereinafter referred to as five grain peptides) is direct and basal diet mixture in 8.0%, 12.0%, 16.0% and 20.0% ratio, and feed after extra interpolation, 40 days experimental periods, result of the test as shown in Tables 3 and 4.
Table 3 basal diet composition and nutrition
Table 4 additionally adds feeding experiment Comparative result
Can be found out by table 4: five grain peptides are direct and basal diet mixture in 8.0%, 12.0%, 16.0% and 20.0% ratio, feed after extra interpolation, the daily gain of piglet improves 4.42%, 6.30%, 10.09% and 11.55% than control group respectively; Feed intake improves 5.16%, 7.05%, 9.31% and 10.72% than control group respectively; But each group feedstuff-meat ratio is there are no significant change.
Diarrhea rate significantly reduces 33.33%, 44.47%, 66.67% and 66.67% than control group respectively; Ileum lactic acid bacterium number significantly improves 2.82%, 6.04%, 7.34% and 9.86% than control group respectively.
Result shows: five grain peptides directly with basal diet mixture, extra interpolation use amount, when 8.0-20.0%, obviously can improve the production performance of weanling pig, promote weanling pig growth, improve daily gain, increase feed intake and enteron aisle lactic acid bacterium number, and reduce Escherichia coli quantity.
Four, the feeding glycolipidpeptide feeding effect of embodiment 3 preparation
In Anhui, body condition, parity, body weight and the expected date of childbirth close healthy Duroc milking sow 160 is chosen in branch, safe and sound farm bloc commodity pig farm one, be divided into 4 groups at random, often organize 5 repetitions, each repetition 8 sows, to study the actual feeding effect of five grain peptides, i.e. control group, glucose group, 10% 5 grain peptide group, 20% 5 grain peptide group.Test and terminate to during piglet wean in 21 days 108 days from gestation.
4 groups of test sucking pig basal diet compositions are all identical with nutrition.Contrast A group neither adds glucose, does not also add five grain peptides; Contrast B group interpolation 3% glucose, but do not add five grain peptides; 10% 5 grain peptide group adds 10% 5 grain peptide; 20% 5 grain peptide group adds 20% 5 grain peptide.Refer to table 5.
Test sow house is roof double-pitch type, cement flooring, and ventilation and lighting is better, natural lighting on daytime, ventilation, night lights illumination, normal immunological and management.Pig house temperature controls to and is not less than 15 DEG C, and humid control is at 55-65%.Accomplish that environmental condition is consistent, with at a draft supporting member's management, free choice feeding and drinking-water as far as possible.
Table 5 tests daily ration composition and nutrition
Note: 4% premix contains calcium formate, calcium monohydrogen phosphate, salt, lysine, methionine, vitamin, trace element and medicine etc.
Table 6 five grain peptide is on the impact of milking sow production performance
From table 5 and 6, after using glucose, 10% 5 grain peptide and 20% 5 grain peptide to feed in lactating swine diets 27-28 days, the daily gain of piglet improves 4.05%, 13.79% and 16.32% (p < 0.05 or p < 0.01) than control group respectively, and within 21 days, age in days survival rate improves 4.02%, 7.09% and 7.67% (p < 0.05) respectively.
The feed intake of milking sow improves 5.89%, 11.31% and 13.16% (p < 0.05) than control group respectively, and sow postweaning estrus interval shortens 4.00%, 21.33% and 18.67% (p < 0.05) than control group.Result shows: five grain peptides can significantly improve the reproductive performance of milking sow.
Five, embodiment 5 and 6 prepare feeding glycolipidpeptide in main active substances content, in table 7.
Feeding glycolipidpeptide Middle nutrition content of material prepared by table 7 embodiment 5 and 6
Six, embodiment 7 and 8 prepare feeding glycolipidpeptide in main active substances content, in table 8.
Feeding glycolipidpeptide Middle nutrition content of material prepared by table 8 embodiment 7 and 8
Seven, embodiment 14 prepare feeding glycolipidpeptide in main active substances content, in table 9.
Feeding glycolipidpeptide Middle nutrition content of material prepared by table 9 embodiment 14
Eight, embodiment 15 prepare feeding glycolipidpeptide in main active substances content, in table 10.
Feeding glycolipidpeptide Middle nutrition content of material prepared by table 10 embodiment 15
Above content is only citing made for the present invention and explanation; affiliated those skilled in the art make various amendment to described specific embodiment or supplement or adopt similar mode to substitute; only otherwise depart from the design of invention or surmount this scope as defined in the claims, protection scope of the present invention all should be belonged to.
Claims (8)
1. a preparation method for feeding glycolipidpeptide, is characterized in that, step is as follows:
1), by the grain trough containing starch through α-amylaseliquefied, saccharification, Separation of Solid and Liquid obtains grain polysaccharide liquid and cereal solid content wet basis;
2), by enzymolysis, emulsification, homogeneous after a small amount of grain polysaccharide liquid or the mixing of clean water, compound feeding oil, emulsifying agent, lipase, antioxidant and liposoluble vitamin, obtained breast separates fat slurry;
3), by the mixing of cereal solid content wet basis, protein feeds, clean water, mixed bacteria liquid and protease, fermentation, enzymolysis, obtained glycolysis slag dregs of rice peptide;
4), the preparation of feeding glycolipidpeptide
After grain polysaccharide liquid, breast being separated fat slurry, two or three absorption in glycolysis slag dregs of rice peptide, absorption carrier feed, mixing, oven dry, then be mixed into anti-caking agent and obtain feeding glycolipidpeptide;
Or;
Grain polysaccharide liquid and breast separated fat slurry adsorbs separately with glycolysis slag dregs of rice peptide, mixes, after drying, then be mixed into anti-caking agent and obtain feeding glycolipidpeptide;
Or;
By grain polysaccharide liquid, breast separates fat slurry, glycolysis slag dregs of rice peptide adsorbs, mixes together with absorption carrier feed, after drying, then be mixed into anti-caking agent and obtain feeding glycolipidpeptide;
Or;
Grain polysaccharide liquid and breast are separated fat starch drying respectively and make Icing Sugar and cosmetics, glycolysis slag dregs of rice Gly-His-Lys is made in the drying of glycolysis slag dregs of rice peptide, then glycolysis slag dregs of rice Gly-His-Lys, Icing Sugar and cosmetics are mixed, then be mixed into anti-caking agent and obtain feeding glycolipidpeptide;
Or;
After grain polysaccharide liquid and breast being separated the mixing of fat slurry, glycolipid powder is made in drying, and glycolysis slag dregs of rice Gly-His-Lys is made in the drying of glycolysis slag dregs of rice peptide, then by after glycolysis slag dregs of rice Gly-His-Lys and the mixing of glycolipid powder, then is mixed into anti-caking agent and obtains feeding glycolipidpeptide.
2. the preparation method of feeding glycolipidpeptide according to claim 1, is characterized in that, step 1) in the preparation process of grain polysaccharide liquid and cereal solid content wet basis as follows:
1. the grain trough impurity elimination, by containing starch, pulverizing, be 100: 100 ~ 10000 add clean water by the weight ratio of grain trough and water, be modulated into enzymolysis slurries, be warming up to 50 ~ 150 DEG C of liquefaction, with 20% aqua calcis, pH be adjusted to 2.0 ~ 12.0;
Grain trough containing starch is corn, wheat, crack rice or the dilated product of rice, paddy, flour, oat, barley and Ipomoea batatas or each cereal, and weight ratio is 100: 0 ~ 300: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 500;
2., by 0.1 ~ 100U/g substrate butt dosage, AMS is added, liquefaction enzymolysis 0.05 ~ 24h;
3., be cooled to 30 ~ 90 DEG C, with 80% lactic acid solution or citric acid solution, pH be adjusted to 1.0 ~ 8.0;
4., carbohydrase is added by 0.05 ~ 10000U/g substrate butt dosage, zytase is added by 0.05 ~ 1200U/g substrate butt dosage, dextranase is added by 0.05 ~ 1200U/g substrate butt dosage, after enzymatic saccharification 0.05 ~ 96h, isolate cereal solid content wet basis and grain polysaccharide liquid through solid-liquid separating equipment.
3. the preparation method of feeding glycolipidpeptide according to claim 2, is characterized in that,
Step corn 1., wheat, crack rice or rice, paddy, flour, oat, barley and Ipomoea batatas weight ratio be 100: 0 ~ 30: 0 ~ 50: 0 ~ 50: 0 ~ 50: 0 ~ 50: 0 ~ 50: 0 ~ 50;
Step condensing temperature is 1. 65 ~ 105 DEG C, and liquefaction enzymolysis pH is 5.8 ~ 6.6;
Step AMS addition is 2. 8.0 ~ 12U/g substrate butt, and liquefaction enzymolysis time is 2.0 ~ 4h;
Step 3. be cooled to 60 ~ 70 DEG C, pH is adjusted to 4.0 ~ 5.0;
The step enzymatic saccharification time is 4. 2 ~ 48h.
4. the preparation method of feeding glycolipidpeptide according to claim 1, it is characterized in that, step 2) in compound feeding oil be soybean oil, corn oil, palm oil, rice bran oil and fish oil, by a small amount of grain polysaccharide liquid or clean water, soybean oil, corn oil, palm oil, rice bran oil, fish oil, emulsifying agent, lipase, antioxidant and liposoluble vitamin are 100: 0 ~ 600: 0 ~ 600: 0 ~ 600: 0 ~ 600: 0 ~ 600: 0.005 ~ 50: 0.00005 ~ 5.0: 0.00001 ~ 0.5: 0.005 ~ 50 to mix by weight, make fatty sugared slag liquid, enzymolysis is carried out after being filtered by sugared for fat slag liquid, emulsification, homogeneous, make breast and separate fat slurry.
5. the preparation method of feeding glycolipidpeptide according to claim 4, it is characterized in that, a small amount of grain polysaccharide liquid or clean water, soybean oil, corn oil, palm oil, rice bran oil, fish oil, emulsifying agent, lipase, antioxidant and liposoluble vitamin are 100: 20 ~ 500: 20 ~ 200: 10 ~ 100: 10 ~ 100: 10 ~ 100: 0.5 ~ 10: 0.001 ~ 2.0: 0.0001 ~ 0.1: 0.05 ~ 5 by weight.
6. the preparation method of feeding glycolipidpeptide according to claim 1, is characterized in that, step 3) in the preparation process of glycolysis slag dregs of rice peptide as follows:
A, by after protein feeds impurity elimination, pulverizing, be 100: 0 ~ 3000: 0 ~ 6000: 0.01 ~ 100 mixing by the weight ratio of cereal solid content wet basis, protein feeds, clean water and mixed bacteria liquid, puddle evenly and be warmed up to 15 ~ 50 DEG C;
Protein feeds is the dilated product of dregs of beans, soybean, pea, red bean, cotton dregs, rapeseed dregs, fish meal, blood meal, plasma proteins, wheat gluten flour, corn protein powder, rice protein powder, DDGS or each feed, and weight ratio is 100: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 500: 0 ~ 300: 0 ~ 300: 0 ~ 300: 0 ~ 300: 0 ~ 300: 0 ~ 300: 0 ~ 300;
Yeast seeds liquid in mixed bacteria liquid, lactic acid bacteria culturers liquid, Bacillus subtilis strain liquid are 100: 0 ~ 800: 0 ~ 800 containing spore number of viable ratio;
B, by 0.005 ~ 2000U/g substrate wet basis dosage, add microbial protease;
C, mix after fermentation and enzymolysis 8 ~ 132h, make glycolysis slag dregs of rice peptide.
7. the preparation method of feeding glycolipidpeptide according to claim 6, is characterized in that,
The weight ratio of the dregs of beans of steps A, soybean, pea, red bean, cotton dregs, rapeseed dregs, fish meal, blood meal, plasma proteins, wheat gluten flour, corn protein powder, rice protein powder and DDGS is 100: 0 ~ 30: 0 ~ 50: 0 ~ 50: 0 ~ 50: 0 ~ 50: 0 ~ 30: 0 ~ 30: 0 ~ 30: 0 ~ 30: 0 ~ 50: 0 ~ 50: 0 ~ 50;
The weight ratio of the cereal solid content wet basis of steps A, protein feeds, clean water and mixed bacteria liquid is 100: 0 ~ 500: 0 ~ 1000: 5 ~ 20 mixing, puddles evenly and is warmed up to 28 ~ 35 DEG C;
The yeast seeds liquid of steps A, lactic acid bacteria culturers liquid, Bacillus subtilis strain liquid be 100: 200 ~ 300: 50 ~ 100 containing spore number of viable ratio;
The microbial protease additive capacity of step B is 60.0 ~ 120U/g substrate wet basis;
The fermentation of step C and enzymolysis time are 36 ~ 84h.
8. the preparation method of feeding glycolipidpeptide according to claim 1, it is characterized in that, step 4) in absorption carrier feed be corn, paddy, crack rice or rice, wheat, wheat bran, rice bran, flour, oat, barley, Ipomoea batatas, soybean, dregs of beans, pea, red bean, cotton dregs, rapeseed dregs, fish meal, blood meal, plasma proteins, hyperglobulinemia, lactose, whey powder, DDDS, corn protein powder, rice protein powder, wheat gluten flour, soybean protein isolate, FSPC, fermentation grouts, starch, maltodextrin, one or more mixture in the dilated product of pasture powder and Chinese herbal medicine and each raw material thereof.
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