CN105410166A - Method for storing prunussalicina by lactic acid bacteria fermentation method and prunussalicina fruit semifinished products - Google Patents
Method for storing prunussalicina by lactic acid bacteria fermentation method and prunussalicina fruit semifinished products Download PDFInfo
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- CN105410166A CN105410166A CN201510697876.0A CN201510697876A CN105410166A CN 105410166 A CN105410166 A CN 105410166A CN 201510697876 A CN201510697876 A CN 201510697876A CN 105410166 A CN105410166 A CN 105410166A
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- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 95
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 74
- 238000000034 method Methods 0.000 title claims abstract description 45
- 241000894006 Bacteria Species 0.000 title claims abstract description 39
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 37
- 239000004310 lactic acid Substances 0.000 title claims abstract description 37
- 238000000855 fermentation Methods 0.000 title abstract description 5
- 230000004151 fermentation Effects 0.000 title abstract description 5
- 239000011265 semifinished product Substances 0.000 title abstract description 5
- 240000005049 Prunus salicina Species 0.000 title abstract 4
- 235000012904 Prunus salicina Nutrition 0.000 title abstract 4
- 235000003681 Prunus ussuriensis Nutrition 0.000 title abstract 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims abstract description 17
- NCXMLFZGDNKEPB-FFPOYIOWSA-N natamycin Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C[C@@H](C)OC(=O)/C=C/[C@H]2O[C@@H]2C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 NCXMLFZGDNKEPB-FFPOYIOWSA-N 0.000 claims abstract description 17
- 229960003255 natamycin Drugs 0.000 claims abstract description 17
- 235000010199 sorbic acid Nutrition 0.000 claims abstract description 17
- 239000004334 sorbic acid Substances 0.000 claims abstract description 17
- 229940075582 sorbic acid Drugs 0.000 claims abstract description 17
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 16
- 239000008103 glucose Substances 0.000 claims abstract description 16
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 14
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 14
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 14
- 239000000203 mixture Substances 0.000 claims abstract description 10
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 3
- 241000555675 Gentiana triflora Species 0.000 claims description 49
- 238000004321 preservation Methods 0.000 claims description 48
- 150000003839 salts Chemical class 0.000 claims description 21
- 235000010298 natamycin Nutrition 0.000 claims description 16
- 239000004311 natamycin Substances 0.000 claims description 16
- 239000002054 inoculum Substances 0.000 claims description 15
- 244000199866 Lactobacillus casei Species 0.000 claims description 9
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 9
- 229940017800 lactobacillus casei Drugs 0.000 claims description 9
- 238000005303 weighing Methods 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 235000013305 food Nutrition 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- 238000003860 storage Methods 0.000 abstract description 4
- 238000005554 pickling Methods 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- 235000002639 sodium chloride Nutrition 0.000 abstract 2
- 239000011780 sodium chloride Substances 0.000 abstract 2
- 241000186660 Lactobacillus Species 0.000 abstract 1
- NCXMLFZGDNKEPB-UHFFFAOYSA-N Pimaricin Natural products OC1C(N)C(O)C(C)OC1OC1C=CC=CC=CC=CCC(C)OC(=O)C=CC2OC2CC(O)CC(O)(CC(O)C2C(O)=O)OC2C1 NCXMLFZGDNKEPB-UHFFFAOYSA-N 0.000 abstract 1
- 229960001031 glucose Drugs 0.000 abstract 1
- 229940039696 lactobacillus Drugs 0.000 abstract 1
- 241000235342 Saccharomycetes Species 0.000 description 7
- 239000010495 camellia oil Substances 0.000 description 7
- 239000012530 fluid Substances 0.000 description 7
- 240000008574 Capsicum frutescens Species 0.000 description 5
- 235000015110 jellies Nutrition 0.000 description 5
- 239000008274 jelly Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- LSNNMFCWUKXFEE-UHFFFAOYSA-L sulfite Chemical compound [O-]S([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-L 0.000 description 5
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 4
- 239000005864 Sulphur Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 229930002877 anthocyanin Natural products 0.000 description 3
- 235000010208 anthocyanin Nutrition 0.000 description 3
- 239000004410 anthocyanin Substances 0.000 description 3
- 150000004636 anthocyanins Chemical class 0.000 description 3
- 235000012055 fruits and vegetables Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 235000015067 sauces Nutrition 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 235000002566 Capsicum Nutrition 0.000 description 2
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical group O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000001390 capsicum minimum Substances 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 235000015598 salt intake Nutrition 0.000 description 2
- 235000012976 tarts Nutrition 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000021110 pickles Nutrition 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
- A23B7/155—Microorganisms; Enzymes; Antibiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the technical field of food processing, and discloses a method for storing prunussalicina by a lactic acid bacteria fermentation method and prunussalicina fruit semifinished products. The method disclosed by the invention comprises the following steps of uniformly mixing fruits and water in a scientific ratio to obtain a mixture, adding sorbic acid, pimaricin, glucose and table salt, then inoculating lactobacillus plantarum and/or lactobacillus caseia, and performing fermentative storage. The fruit semifinished products stored by the method disclosed by the invention are dark red in color and intact in fruits, the hardness meets requirements, the safety of the fruit semifinished products can be improved, the consumption of the table salt is reduced, the production cost is reduced, and besides, environmental pollution caused by drainage of pickling liquid with high salinity is reduced.
Description
Technical field
The present invention relates to food processing technology field, more specifically, relate to a kind of method and Gentiana triflora fruit base of fermented by lactic acid bacteria preservation Gentiana triflora.
Background technology
Gentiana triflora meat production is high, and pulp is aubergine, is the first-class fruit of processing fruit jelly.By disposable for Gentiana triflora making fruit base on producing, as semi-finished product storage, for the preserved fruit process anniversary.Easily there is brown stain in fruits and vegetables, not only affect organoleptic quality, and cause nutritive loss in storage and processing.The color of red plum fruit, mainly determined by the content of anthocyanin, the degraded of anthocyanin is very large to Gentiana triflora Color influences.Current production adopts sulphite preservation fruit base, but the method misapplication can cause the sulfur dioxide residue in product to exceed standard, this is the underproof main cause of current fruit jelly product quality.A large amount of uses of sulphite also can destroy anthocyanin, reduce the nutritive value of fruit base.The salt of more than 20% to be added to strengthen preservation effect while sulphite preservation fruit base, cause salt content in pickling liquid high, increase cost, during discharge, severe contamination is produced to environment simultaneously.
Lactic acid bacteria is that a class can be fermented and utilized glucide and produce the bacterium of a large amount of lactic acid, and it is the flora that a class is useful to human body.Lactobacillus-fermented can produce the metabolins such as a large amount of organic acids, alcohols and each seed amino acid, and research shows to have suppression spoilage organisms, improve the physiological function such as digestibility, anti-cancer.Nowadays, it has been recognized that the physiological function of lactic acid bacteria, lactobacillus-fermented is widely used in food processing, the aspects such as such as dairy products, cream, vegetable deep-processing.
Food boils by the lactic acid bacteria application in fruits and vegetables usually, shred or carry out lactobacillus-fermented after fragmentation again pickles or squeezes the juice.Practical application effect and concrete application, application conditions and yeasting closely related, under different application, application conditions and yeasting, the fermentation of acquisition is significantly different.
Application publication number be CN104543965A patent discloses a kind of method utilizing lactobacillus-fermented and tea oil preservation thick chilli sauce, mix after capsicum fragmentation with other auxiliary materials and streptococcus acidi lactici fermented solution, sealing, ferment and add tea oil after 1 ~ 2 month thick chilli sauce is covered, added a tea oil every 3 months, the thick chilli sauce color obtained is good, quality is crisp, long shelf-life.Technical scheme disclosed in this patent based on the synergy of tea oil and lactic acid bacteria, and needs capsicum broken, and covers with tea oil, regularly adds tea oil.Because the preservation of fruit base requires that fruit is complete, can not be broken, its inside and outside all has preservation requirement, and in hardness, have strict requirement, and fruit base is not suitable for adopting tea oil, is not suitable for the fermentative preservation carrying out fruit base in this way.
Summary of the invention
The technical problem to be solved in the present invention overcomes the deficiency that preservation technology produced by existing Gentiana triflora fruit base, a kind of method of fermented by lactic acid bacteria preservation Gentiana triflora is provided, the traditional sulphur adopting this method to substitute fruit base hides method, the security of fruit base can be improved well, reduce salt consumption, reduce costs, reduce the pollution of discharging of waste liquid.
Another technical problem that the present invention will solve is to provide the Gentiana triflora fruit base that described method prepares.
Object of the present invention is achieved through the following technical solutions:
A kind of method of fermented by lactic acid bacteria preservation Gentiana triflora is provided, specifically comprises the following steps:
S1. Gentiana triflora is cleaned, drain the water after mix with water, add sorbic acid and natamycin;
S2. in step S1 mixture, add glucose and salt, the addition of described salt is 2.5% ~ 5.0% of Gentiana triflora weight;
S3. in step S2 mixture, access lactic acid bacteria, by the weight of Gentiana triflora, total inoculum concentration is mixing after 2 ~ 6% inoculations, sealing, 37 DEG C of fermentative preservations.
Preferably, mixing with water described in step S1 be mass ratio according to Gentiana triflora and water is 1:1 ~ 1:2, described ratio more preferably 1:1.8.
Preferably, the addition of sorbic acid described in step S1, for press Gentiana triflora weighing scale, adds 0.05% sorbic acid; The addition of described natamycin, according to Gentiana triflora weighing scale, adds the natamycin of 0.02%.Under the scientific and reasonable interpolation sorbic acid of the present invention and natamycin condition, both can suppress varied bacteria growing well, the remarkable result of fermentative preservation can be ensured again.
Preferably, the addition of glucose described in step S2 is 3% ~ 5% of Gentiana triflora weight.The addition of described glucose more preferably 3.34%, the addition of described salt more preferably 4.97%.The glucose sugar adding reasonable amount provides fermentation substrate for the fermentation of follow-up lactic acid bacteria; Add the salt of reasonable amount, both can suppress varied bacteria growing, fruit base hardness can be maintained again well.The present invention, by the optimization of overall technical architecture, reduces the consumption of salt significantly.
Preferably, lactic acid bacteria described in step S3 is Lactobacillus plantarum and/or Lactobacillus casei, and according to the two mixture, the ratio of Lactobacillus plantarum and Lactobacillus casei is 3:1.The cultural method of described lactic acid bacteria is that lactic acid bacteria is accessed MRS culture medium, 37 DEG C of quiescent culture 24h; Inoculum concentration is 2%.
Preferably, total inoculum concentration of step S3 is 3.54%.
The invention provides one preferably embodiment: according to fruit: water=1:1.8(mass ratio) ratio mixing, according to Gentiana triflora weighing scale, add 0.05% sorbic acid, 0.02% natamycin, 3.34% glucose, 4.97% salt, then be 3:1 and total inoculum concentration according to the ratio of Lactobacillus plantarum and Lactobacillus casei be 3.54% inoculating lactic acid bacterium, fermentative preservation.
Preferably, the method for the invention adopts PA/PE packaging bag to carry out preservation, in 37 DEG C of storage fermentative preservations after sealing.
The invention provides the Gentiana triflora fruit base that described method prepares.
The present invention has following beneficial effect:
Current production adopts sulphite preservation fruit base, but the method misapplication, the sulfur dioxide residue in product can be caused to exceed standard, and this is the underproof main cause of current fruit jelly product quality.The excessive use of sulphite also causes the natural colouring matter heavy damage in fruit, reduces the nutritive value of fruit.And in current method for preserving, the use amount of salt is very large, both affects mouthfeel, causes again waste and pollutes, also bringing hidden danger to the health of eater.
The present invention selects suitable material combination, builds the yeasting optimized, and inoculates suitable lactic acid bacteria and ferments, play the effect extending fruit base preservation term in Lee fruit base.In the process of lactobacillus-fermented fruits and vegetables, produce multiple organic acid, vitamin, amino acid and enzyme material, come soft tart flavour and unique fragrance to fruit blank strip, the growth of spoilage organisms and pathogen can be suppressed, extend the shelf-life of vegetable product.
The present invention is especially for Gentiana triflora, sum up and obtain scientific and reasonable technology and condition, achieve and adopt fermented by lactic acid bacteria to carry out preservation Gentiana triflora, add without sulphur in method, the fruit base color of preservation is dark red, fruit is intact, hardness meets the requirements, and improves the security of fruit base, reduces salt consumption, reduce production cost, reduce high salinity pickling liquid simultaneously and discharge the environmental pollution caused.Concrete beneficial effect is summarized as follows:
(1) the present invention adds without sulphur, the security of significant increase fruit jelly fruit base.And the use amount of salt is only 9.94% of traditional sulphur preserving process, avoids waste and pollute, meanwhile, avoiding excessive use salt brings healthy aspect hidden danger to eater.
(2) adopt the Gentiana triflora color of the inventive method preservation dark red, fruit is intact, obtains desirable hardness and keeps effect, meet related request.The inventive method is applied to the preservation of fruit jelly fruit base, safe and reliable, simple.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.Unless stated otherwise, the raw material that adopts of the present invention and equipment are raw material and the equipment of this area usual sources and use.
The lactic acid bacteria that the present embodiment adopts is conventional commercial, and can be Lactobacillus plantarum and/or Lactobacillus casei, the cultural method of described lactic acid bacteria be that lactic acid bacteria is accessed MRS culture medium, 37 DEG C of quiescent culture 24h; Inoculum concentration is 2%.Described MRS culture medium is the MRS culture medium that this area routine uses.
embodiment 1
S1. Gentiana triflora is cleaned and drain the water, to mix with mass ratio 1:1.8 with water and pack.Heavy by fruit, add 0.05% sorbic acid and 0.02% natamycin respectively.
S2. heavy by fruit, add 3% glucose and 5.0% salt.
S3. then access Lactobacillus plantarum, heavy by fruit, inoculum concentration is 2%.After mixing, bag is sealed, 37 DEG C of fermentative preservations.
Fruit base a value initial value is 29.87, and hardness is 3.21N.Adopt the fruit base after the present embodiment method preservation 30d, a value is 16.39, and hardness is 1.86N.Fruit base color is redder, and fruit is intact, and hardness meets the requirements.In zymotic fluid, saccharomycete quantity is 4.5 × 10
4cFU/mL.
embodiment 2
(1) Gentiana triflora is cleaned drain the water, to mix with mass ratio 1:1.8 with water and pack.Heavy by fruit, add 0.05% sorbic acid and 0.02% natamycin respectively.
(2) heavy by fruit, add 3% glucose and 5.0% salt.
(3) then access Lactobacillus plantarum, heavy by fruit, total inoculum concentration is 4%.
(4), after mixing, bag is sealed, 37 DEG C of fermentative preservations.
Fruit base a value initial value is 29.87, and hardness is 3.21N.Fruit base after this method preservation 30d, a value is 17.05, and hardness is 1.36N.Fruit base color is redder, and fruit is intact, and hardness meets the requirements.In zymotic fluid, saccharomycete quantity is 2.5 × 10
4cFU/mL.
embodiment 3
(1) Gentiana triflora is cleaned drain the water, to mix with mass ratio 1:1.8 with water and pack.Heavy by fruit, add 0.05% sorbic acid and 0.02% natamycin respectively.
(2) heavy by fruit, add 3% glucose and 5.0% salt.
(3) then access Lactobacillus plantarum, heavy by fruit, total inoculum concentration is 6%.
(4), after mixing, bag is sealed, 37 DEG C of fermentative preservations.
Fruit base a value initial value is 29.87, and hardness is 3.21N.Fruit base after this method preservation 30d, a value is 19.35, and hardness is 1.25N.Fruit base color is redder, and fruit is intact, and hardness meets the requirements.In zymotic fluid, saccharomycete quantity is 2.2 × 10
3cFU/mL.
embodiment 4
(1) Gentiana triflora is cleaned drain the water, to mix with mass ratio 1:1.8 with water and pack.Heavy by fruit, add 0.05% sorbic acid and 0.02% natamycin respectively.
(2) heavy by fruit, add 3% glucose and 2.5% salt.
(3) then access Lactobacillus plantarum, heavy by fruit, total inoculum concentration is 4%.
(4), after mixing, bag is sealed, 37 DEG C of fermentative preservations.
Fruit base a value initial value is 29.87, and hardness is 3.21N.Fruit base after this method preservation 30d, a value is 18.01, and hardness is 1.32N.Fruit base color is redder, and fruit is intact, and hardness meets the requirements.In zymotic fluid, saccharomycete quantity is 5.0 × 10
4cFU/mL.
embodiment 5
(1) Gentiana triflora is cleaned drain the water, to mix with mass ratio 1:1.8 with water and pack.Heavy by fruit, add 0.05% sorbic acid and 0.02% natamycin respectively.
(2) heavy by fruit, add 5% glucose and 5% salt.
(3) then access Lactobacillus plantarum, heavy by fruit, total inoculum concentration is 4%.
(4), after mixing, bag is sealed, 37 DEG C of fermentative preservations.
Fruit base a value initial value is 29.87, and hardness is 3.21N.Fruit base after this method preservation 30d, a value is 18.56, and hardness is 1.25.Fruit base color is redder, and fruit is intact, and hardness meets the requirements.In zymotic fluid, saccharomycete quantity is 3.5 × 10
4cFU/mL.
embodiment 6
(1) Gentiana triflora is cleaned drain the water, to mix with mass ratio 1:1.8 with water and pack.Heavy by fruit, add 0.05% sorbic acid and 0.02% natamycin respectively.
(2) heavy by fruit, add 3% glucose and 5% salt.
(3) then access Lactobacillus casei, heavy by fruit, total inoculum concentration is 4%.
(4), after mixing, bag is sealed, 37 DEG C of fermentative preservations.
Fruit base a value initial value is 29.87, and hardness is 3.21N.Fruit base after this method preservation 30d, a value is 13.21, and hardness is 2.13.Fruit base color is redder, and fruit is intact, and hardness meets the requirements.In zymotic fluid, saccharomycete quantity is 7.5 × 10
5cFU/mL.
embodiment 7
(1) Gentiana triflora is cleaned drain the water, to mix with mass ratio 1:1.8 with water and pack.Heavy by fruit, add 0.05% sorbic acid and 0.02% natamycin respectively.
(2) heavy by fruit, add 3.34% glucose and 4.97% salt.
(3) then access Lactobacillus plantarum and Lactobacillus casei, the two ratio is 3:1, and heavy by fruit, total inoculum concentration is 3.54%.
(4), after mixing, bag is sealed, 37 DEG C of fermentative preservations.
Fruit base a value initial value is 29.87, and hardness is 3.21N.Fruit base after this method preservation 90d, a value is 17.00, and hardness is 1.51N.Fruit base color is dark red, and fruit is intact, and hardness meets the requirements, and fruit blank strip comes soft tart flavour and unique fragrance.In zymotic fluid, saccharomycete quantity is 6.0 × 10
4cFU/mL.
Claims (10)
1. a method for fermented by lactic acid bacteria preservation Gentiana triflora, is characterized in that, specifically comprises the following steps:
S1. Gentiana triflora is cleaned after draining the water and mix with water, add sorbic acid and natamycin;
S2. in step S1 mixture, add glucose and salt, the addition of described salt is 2.5% ~ 5.0% of Gentiana triflora weight;
S3. in step S2 mixture, access lactic acid bacteria, by the weight of Gentiana triflora, total inoculum concentration is mixing after 2 ~ 6% inoculations, sealing, 37 DEG C of fermentative preservations.
2.
according to claim 1, the method for fermented by lactic acid bacteria preservation Gentiana triflora, is characterized in that, mixing with water described in step S1 is be that 1:1 ~ 1:2 mixes according to Gentiana triflora and the mass ratio of water; Be preferably that 1:1.8 mixes according to Gentiana triflora and the mass ratio of water.
3.
according to claim 1, the method for fermented by lactic acid bacteria preservation Gentiana triflora, is characterized in that, the addition of sorbic acid described in step S1, for press Gentiana triflora weighing scale, adds 0.05% sorbic acid.
4.
according to claim 1, the method for fermented by lactic acid bacteria preservation Gentiana triflora, is characterized in that, the addition of described natamycin, according to Gentiana triflora weighing scale, adds the natamycin of 0.02%.
5.
according to claim 1, the method for fermented by lactic acid bacteria preservation Gentiana triflora, is characterized in that, the addition of glucose described in step S2 is 3% ~ 5% of Gentiana triflora weight.
6.
according to claim 1, the method for fermented by lactic acid bacteria preservation Gentiana triflora, is characterized in that, lactic acid bacteria described in step S3 is Lactobacillus plantarum and/or Lactobacillus casei.
7.
according to claim 6, the method for fermented by lactic acid bacteria preservation Gentiana triflora, is characterized in that, lactic acid bacteria described in step S3 is Lactobacillus plantarum and Lactobacillus casei is proportionally the mixture of 3:1 mixing.
8.
according to claim 6 or 7, the method for fermented by lactic acid bacteria preservation Gentiana triflora, is characterized in that, the cultural method of described lactic acid bacteria is that lactic acid bacteria is accessed MRS culture medium, 37 DEG C of quiescent culture 24h; Inoculum concentration is 2%.
9.
according to the method for the arbitrary described fermented by lactic acid bacteria preservation Gentiana triflora of claim 1 to 8, it is characterized in that, according to fruit: water=1:1.8(mass ratio) ratio mixing, according to Gentiana triflora weighing scale, add 0.05% sorbic acid, 0.02% natamycin, 3.34% glucose, 4.97% salt, then be 3:1 and total inoculum concentration according to the ratio of Lactobacillus plantarum and Lactobacillus casei be 3.54% inoculating lactic acid bacterium, fermentative preservation.
10.
the Gentiana triflora fruit base that described in any one of claim 1 to 9, method prepares.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111296560A (en) * | 2020-02-28 | 2020-06-19 | 华南农业大学 | Fermentation preservation method of Sanhua plum fruit blank |
CN111793582A (en) * | 2020-07-27 | 2020-10-20 | 广东省农业科学院蚕业与农产品加工研究所 | Sanhua plum wine deacidification microbial inoculum and application thereof and Sanhua plum wine production method |
CN112674161A (en) * | 2020-12-23 | 2021-04-20 | 广东康辉集团有限公司 | Preparation method of fruit blank color protection preservation, high-anthocyanin fruit blank and application |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102630796A (en) * | 2012-05-10 | 2012-08-15 | 陈鹏 | Technology for processing glace fruit through biological method |
CN102726515A (en) * | 2012-07-17 | 2012-10-17 | 广东石油化工学院 | Fresh-keeping method of Sanhua plums |
CN104543965A (en) * | 2015-01-15 | 2015-04-29 | 湖南农业大学 | Method for preserving chilli sauce by employing lactobacillus fermentation and tea oil |
-
2015
- 2015-10-26 CN CN201510697876.0A patent/CN105410166A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102630796A (en) * | 2012-05-10 | 2012-08-15 | 陈鹏 | Technology for processing glace fruit through biological method |
CN102726515A (en) * | 2012-07-17 | 2012-10-17 | 广东石油化工学院 | Fresh-keeping method of Sanhua plums |
CN104543965A (en) * | 2015-01-15 | 2015-04-29 | 湖南农业大学 | Method for preserving chilli sauce by employing lactobacillus fermentation and tea oil |
Non-Patent Citations (2)
Title |
---|
刘书亮等: "《纳他霉素与山梨酸复配改良MRS培养基的研究》", 《中国酿造》 * |
韩新锋等: "《发酵肉制品中乳酸菌计数培养基的优化》", 《中国酿造》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111296560A (en) * | 2020-02-28 | 2020-06-19 | 华南农业大学 | Fermentation preservation method of Sanhua plum fruit blank |
CN111296560B (en) * | 2020-02-28 | 2023-03-14 | 华南农业大学 | Fermentation preservation method of Sanhua plum fruit blank |
CN111793582A (en) * | 2020-07-27 | 2020-10-20 | 广东省农业科学院蚕业与农产品加工研究所 | Sanhua plum wine deacidification microbial inoculum and application thereof and Sanhua plum wine production method |
CN112674161A (en) * | 2020-12-23 | 2021-04-20 | 广东康辉集团有限公司 | Preparation method of fruit blank color protection preservation, high-anthocyanin fruit blank and application |
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