The processing method of the black garlic products of a kind of rich selenium
Technical field
The present invention relates to the processing method of the black garlic products of a kind of rich selenium.
Background technology
The nutritive value of garlic (Allium sativum L.) and health-care efficacy generally obtain common people generally acknowledges, its process requirements amount increases day by day, and product application is increasingly extensive.At present, garlic products is mainly take pickled product as main, in addition also have garlic powder, garlic pearls, garlic rice, garlic soy sauce, Garlic beverage, garlic sausage etc., and above product has acid and stink that uncooked garlic stimulates, restrict to a certain extent the development of social demand and process technology, therefore prepare new type functional garlic products most important to maintenance China garlic industry leading position in the international market with its process technology of development, be also the current problem in the urgent need to address of garlic industry simultaneously.Adopt the black garlic of rich selenium of High biotechnology processing, not only removed the peculiar smell of garlic, strengthened taste, and as a kind of novel selenium-rich function garlic product, nutrition is abundanter, biologically active strengthens, and has more application prospect and economic worth.
In Chinese patent CN101731605A, report a kind of production and processing method of black garlic, take selected high-quality garlic as raw material, adopt fermentation box in fermenting cellar, to carry out stepwise fermentation, temperature is 50~80 ℃, humidity is 50%~80%, fermentation time is 30 d, and water-soluble in product, lipid fractions matter, all higher than Common garlic, has strengthened the health-care efficacy of garlic.But in this process, adopt the zymotechnique of multiple stage temperature adjustments and damping, and raw material adopts Common garlic, complicated operation, and fermentation parameter is wayward, and fermentation period is long, is unfavorable for reducing costs.The preparation method of a kind of black garlic providing in CN102450618A is cleaning and dipping after garlic is selected, adopt the progressively mode of cooling, in surrounding, regulate respectively fermentation temperature and humidity, initial temperature and humidity are respectively 80 ± 2 ℃, 80 ± 2%, reduce weekly 5 ℃ and 5% humidity, after-ripening temperature is 25 ± 2 ℃, and relative humidity is 50 ± 2%.This goods oxidation resistance and activate effect improve greatly.The fermentation parameter of this processing technology is more meticulous, and operation is comparatively complicated, and Zymolysis Equipment is had relatively high expectations, and is unfavorable for large-scale production, has the defects such as raw material is common, fermentation period is long simultaneously.
In addition, garlic is the vegetables that have selenium rich ability most, selenium is the essential material that maintains human normal physiological metabolism, and the nutrients such as vitamin in the black garlic of rich selenium in selenium and the garlic of enrichment, allicin, linoleic acid have collaborative oxidation resistant effect, and its health-care efficacy is better than common black garlic.
In sum, produce for the industrialization deep processing of black garlic, development heath-function raw material, low cost, high benefit, strong operability, parameter be easy to control, process technology with short production cycle is most important.
Summary of the invention
The object of this invention is to provide the processing method of the black garlic products of a kind of rich selenium, can overcome the defect of prior art.The present invention selects novel garlic raw material, and processing technology provides a kind of novel functional black garlic product.This product take plantation rich selenium fresh garlic or dry garlic as raw material, adopt raw material pre-treatment, two-period form temperature control fermentation technique, black Garlic Tissue softness, the sour and sweet palatability of preparation, the content of Organic Selenium is original 2~3 times, it is anti-oxidant and remove the ability of free radical higher than relevant report.That the present invention has is simple to operate, cost is low, fermentation time is short, product health-care efficacy advantages of higher.
The processing method of the black garlic products of a kind of rich selenium provided by the invention is take the rich selenium fresh garlic of high-quality or dry garlic as raw material, through the past base of a fruit clean, saline solution infiltrate drain, low temperature pre-freeze, the operations such as inoculating lactic acid probio, prior fermentation, main fermentation, after-ripening are dry, vacuum packaging are made.Process comprises the steps:
1) raw material sorting, selecting the diameter of high-quality is the selenium-enriched garlic of 2~3cm left and right, removes the root base of a fruit, washs with clear water.
2) garlic pretreatment, puts into the garlic after sorting washing in step 1) 0.2~0.3% saline solution and soaks, and puts into refrigerating chamber and carry out the processing of low temperature pre-freeze after draining.
3) inoculating lactic acid bacterium, for obtaining the soft tart flavour of the black garlic of rich selenium, by step 2) the middle good garlic inoculating lactic acid bacterium leavening agent under aseptic condition of pretreatment, the lactobacillus inoculation of inoculation is the mixed bacteria of lactic acid probiotic bifidobacteria, lactobacillus bulgaricus, streptococcus thermophilus.
4) prior fermentation, puts into airtight glass container by the garlic having inoculated in step 3) and puts into incubator, regulates temperature to carry out cold fermentation in early stage; This can promote lactic fermentation in stage on the one hand, can activate on the other hand levulan polymerase in garlic and the activity of protease, increases reduced sugar and amino acid content, to promote the Maillard reaction (Maillard reaction) of lord ferment period.
5) main fermentation, proceeds to constant temperature and humidity incubator by gained garlic in step 4), regulates temperature and humidity to ferment and can obtain black garlic.
6) after-ripening is dry, reduces fermenting cellar temperature and humidity, loosening container cover, and evaporating surplus moisture, the black garlic of rich selenium that can obtain being dried, goods crust is dry, meat blackout, tissue is soft, sweet mouthfeel.
7) vacuum packaging, carries out vacuum packaging by resulting product in step 6), can obtain the black garlic finished product of rich selenium.
In described pretreatment, the saline sook time is 30~60 min, and low temperature pre-freeze temperature is-25~-20 ℃, times 24~48 h.
Described lactic acid bacteria mixed bacteria ratio: Bifidobacterium: lactobacillus bulgaricus: streptococcus thermophilus is 1:1-2:1.
Described prior fermentation temperature is 40~55 ℃, and the time is 2~4 d.
Described lord ferment period temperature is 65~85 ℃, and humidity is 65%~85%, and the time is 17~20 d.
Described after-ripening drying stage temperature is 35~45 ℃, and humidity is 50% left and right, and the time is 2~3 d.
Described vacuum drying is operating as bag distribution packaging, and vacuum is 0.8~1.0 MPa.
The black garlic of rich selenium that the processing method of the black garlic products of a kind of rich selenium provided by the invention finally obtains had both retained original nutritional labeling and the active ingredient of uncooked garlic, the original acid of raw garlic and garlic odour taste are sloughed again, the content of Organic Selenium increases, total phenol content is brought up to 5~8 times of original left and right, and ability anti-oxidant and removing free radical improves greatly.There is following evident characteristic:
1) take the selenium-enriched garlic of planting as material, Common garlic relatively, the health properties of raw material increases; And select fresh ripe garlic or dry garlic all can, raw material be easy to select, cost is reduced greatly.
2) raw material soaks and pre-freeze processing through 0.2~0.3% saline solution, and the time is short, and energy consumption is low, not only can improve the local flavor of black garlic but also can strengthen black garlic tender mouth feel.
3) utilize two-period form fermentation mode, early stage, temperature was low, was beneficial to the vigor of enzyme in lactic fermentation and activation garlic, had increased Maillard and had reacted content amino acid needed and reduced sugar, latter temperature is high and humidity is high, suitable Maillard reaction, 20~25 d can complete fermentation, for Common garlic 30~60 d can complete fermentation, greatly shorten fermentation period, reduce cost, with respect to multistage temperature-variable fermentation mode, simplified operating procedure.
4) the effective nutritional labeling of the black garlic of rich selenium that the present invention obtains does not change in process, and the content of Organic Selenium reaches 0.50~0.54 μ g/g, for 2~3 times of raw material, total phenol content is brought up to 5~8 times of original left and right, and ability anti-oxidant and removing free radical significantly improves.
5) processing technology of the present invention is simple, workable, cost is low, be easy to control, be of high nutritive value, health-care efficacy is strong.There is very high exploitation, using value and economic worth.
The specific embodiment
Below in conjunction with embodiment, the present invention is further described.Here only point out, raw material, reagent and the testing equipment using in the present invention is the commercially available universal product in the time not indicating especially.Described lactic acid bacteria fermenting agent is all lactobacillus products as food additives that sell in market.Described lactic acid bacteria fermenting agent, selects to adopt streptococcus thermophilus, Bifidobacterium and lactobacillus acidophilus, also can adopt existing known other probiotic lactic acid bacterium leavening agent.
Embodiment 1:
1) selecting the ripe garlic of rich selenium of Xiangcheng City of Henan Province service cooperation for agricultural machinery plantation is raw material (selenium-enriched garlic of other domestic professional production all can), select diameter and be 2~3cm left and right without 200, the only head of garlic that rots to go mouldy, remove the root base of a fruit, wash with clear water;
2) by 1) in garlic after the sorting washing saline solution of putting into 5000 mL0.3% soak 60 min, drain, then garlic is neatly placed in pallet, preservative film sealing, puts into refrigerating chamber and processes 24 h in-20 ℃ of pre-freezes;
3) by 2) in process garlic and carry out room temperature and thaw, get 500 mL sterilized waters, according to operation instruction operation preparation lactic acid bacteria (Lactobacillus) mixed culture fermentation agent of the commercially available lactic acid bacteria fermenting agent of business, make Bifidobacterium (bifidobacterium): lactobacillus bulgaricus (Lactobacillus delbrueckii subsp. bulgaricus): streptococcus thermophilus (Lactococcus thermophilus) mixed proportion is to put into the lactic acid bacteria mixed culture fermentation agent that total amount is 0.5 g in 1:2:1(500 mL sterilized water, wherein Bifidobacterium powder 0.125 g, lactobacillus bulgaricus powder 0.25 g, streptococcus thermophilus 0.125 g), then pack aseptic watering can into, spray lactobacillus suspension to garlic, guarantee that garlic surface all speckles with lactic acid bacteria, the garlic having inoculated is respectively charged in 10 L glass containers to sealing,
4) by 3) in airtight glass container in put into incubator, regulate temperature 45 C, cultivate 72 h;
5) by 4) in gained prior fermentation garlic proceed in constant temperature and humidity incubator, regulate temperature 70 C, humidity 70%, fermentation 20 d, can obtain black garlic;
6) loosening glass container lid, reducing temperature is 40 ℃, regulates humidity 60%, evaporating surplus moisture, the black garlic of rich selenium that can obtain being dried, crust is dry, and tissue is soft, sweet mouthfeel;
7) vacuum packaging.By 6) the first bag of middle resulting product 5, carry out vacuum packaging, vacuum is 0.8 MPa.
The effective nutritional labeling of the rich black garlic of selenium does not change in process, and the content of average selenium reaches 0.52 μ g/g(fluorometric determination), for 2 times of raw material, total phenol content on average reaches 4622.57 μ g/g, be 5~8 times of undressed raw material, ability anti-oxidant and that remove free radical is respectively 8 times and 30 times of raw material.
Embodiment 2:
1) selecting the dry garlic of rich selenium of Xiangcheng City of Henan Province service cooperation for agricultural machinery plantation is raw material, selects without the head of garlic that rots to go mouldy, and is divided into garlic clove 2400 lobes, washs with clear water;
2) by 1) in garlic clove after the sorting washing saline solution of putting into 5000 mL 0.3% soak 30 min, drain, then garlic is neatly placed in pallet, preservative film sealing, puts into refrigerating chamber and processes 24 h in-20 ℃ of pre-freezes;
3) by 2) in process garlic and carry out room temperature and thaw, under aseptic condition, get 500 mL sterilized waters, according to the operation instruction operation preparation lactic acid bacteria mixed culture fermentation agent of the commercially available lactic acid bacteria fermenting agent of business, make Bifidobacterium: lactobacillus bulgaricus: streptococcus thermophilus mixed proportion is 1:1:1; Then pack aseptic watering can into, spray lactobacillus suspension to garlic, guarantee that garlic surface all speckles with lactic acid bacteria, the garlic having inoculated is respectively charged in 10 L glass containers to sealing;
4) by 3) in airtight glass container in put into incubator, regulate temperature 45 C, cultivate 48 h;
5) by 4) in gained prior fermentation garlic proceed in constant temperature and humidity incubator, regulate 80 ℃ of temperature, humidity 80%, fermentation 17 d, can obtain black garlic;
6) loosening glass container lid, reducing temperature is 40 ℃, regulates humidity 60%, evaporating surplus moisture, the black garlic bulblet of rich selenium that can obtain being dried, crust is dry, and tissue is soft, sweet mouthfeel;
7) vacuum packaging.By 6) in mono-bag of resulting product 1500g carry out vacuum packaging, vacuum is 0.9 MPa.
The black garlic clove of rich selenium has kept the effective nutritional labeling of original garlic in process, the content of average selenium reaches 0.50 μ g/g(fluorometric determination), be about 2 times of raw material, total phenol content on average reaches 4952.11 μ g/g, be 7 times of undressed raw material, ability anti-oxidant and that remove free radical is respectively 8 times and 10 times of raw material.
The invention provides the processing method of the black garlic products of a kind of rich selenium, those skilled in the art can be by using for reference content herein, the links such as appropriate change process route realize, although method of the present invention is described by preferred embodiment, person skilled obviously can change or reconfigure method as herein described not departing from content of the present invention and scope, realizes final result.Special needs to be pointed out is, all similar replacements and change apparent to those skilled in the artly, they are all deemed to be included in content of the present invention and scope.