CN105393807A - Lithocarpusamygdalifolius(Skan)Hayata-QuercusacutissimaCarruth container seedling grafting breeding method - Google Patents
Lithocarpusamygdalifolius(Skan)Hayata-QuercusacutissimaCarruth container seedling grafting breeding method Download PDFInfo
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- CN105393807A CN105393807A CN201510871324.7A CN201510871324A CN105393807A CN 105393807 A CN105393807 A CN 105393807A CN 201510871324 A CN201510871324 A CN 201510871324A CN 105393807 A CN105393807 A CN 105393807A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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Abstract
The invention discloses a Lithocarpusamygdalifolius(Skan)Hayata-QuercusacutissimaCarruth container seedling grafting breeding method comprising the steps of scion acquisition, grafting time, grating, and post-grafting management; selecting QuercusacutissimaCarruth container nursery stock as stock; cutting strong semi lignifications-lignifying branches with no insect disease from Lithocarpusamygdalifolius(Skan)Hayata seed tree to serve as scion; using cut-grafting method for grafting; carrying out post-grafting management until next spring so as to obtain Lithocarpusamygdalifolius(Skan)Hayata grafted seedlings for afforestation. The method is a breakthrough in Lithocarpusamygdalifolius(Skan)Hayata asexual breeding technology; QuercusacutissimaCarruth stock is used for grafting breeding; QuercusacutissimaCarruth container nursery stocks with excellent female parent economic characters are fast and massively breed, and the stock is prosperous in root system; the QuercusacutissimaCarruth stock has strong adaptation capability for natural dry, wet and cold conditions, the Lithocarpusamygdalifolius(Skan)Hayata seedling can utilize the advantages, so the scion and stock can fast heal and germinate new shoots; the germinated new shoots can grow fast, thus promoting high quality, efficiency and fast development of the Lithocarpusamygdalifolius(Skan)Hayata, and providing better economic benefits, social benefits and ecology benefits.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, relate to the propagation method of a kind of apricot leaf Ke grafting Quercus acutissima container seedling.
Background technology
Apricot leaf Ke (
lithocarpusamygdalifolius (Skan) Hayata) have another name called apricot leaf lithocarpus glaber, red C.-glauca, ridge plum, be Fagaceae (Fagaceae) Lithocarpus (Lithocarpus) arbor, the shoot that spring and summer extracts out and the tender leaf back side is close is rolled up pubescence by yellowish-brown, hair all comes off after autumn.The life of tassel locusta list fringe armpit or many fringes line up panicle, and rhachis is close by pubescence; Every 3 clusters of female flower, sometimes have concurrently single scattered.The nearly spheroidal of acorn-cup, full bag nut, in discontinuous ring-type; The fruit wall of nut is slightly thicker than acorn-cup wall, and nut top is by micro-pubescence.Be distributed in Central Taiwan and south, south Fujian, the southeast, Guangdong be about tropic of cancer areas to the south, Hainan.Being born in height above sea level 1100-2300 rice evergreen broad-leaved forest or needle, Broad-leaved Mixed Forest, is often upper canopy species.
Quercus acutissima (QuercusacutissimaCarruth) has another name called Qinggang, rubber Bowl sets, and be that Fagaceae (Fagaceae) oak belongs to (Quercus) arbor, up to 30 meters, the diameter of a cross-section of a tree trunk 1.3 meters above the ground reaches 1 meter, the dark-grey brown of bark, dark lobe.Be born in the mountain region tailo of height above sea level 60-2200 rice, become small pieces pure forest or mixed forest, positive happiness light, happiness humid climate.Cold-resistant, drought-resistant barren, not water-fast wet, not Salt And Alkali Tolerance, moistening fertile deep, well-drained neutrality to subacidity sandy loam grows best, impeded drainage or chaor should not be planted.Mix with other seeds and hand over and can form good form, deep-rootedness, rudiment power is strong, but not resistance to transplanting.Antipollution, antidusting soil, wind loading rating are all stronger.Life-span is long, can reach 500 ~ 600 years.Produce the provinces and regions such as Liaoning, Hebei, Shanxi, Shandong, Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Henan, Hubei, Hunan, Guangdong, Hainan, Guangxi, Sichuan, Guizhou, Yunnan.
At present, apricot leaf Ke resource is still in wild distribution, but along with keeping the demand of Plant Diversity, large-scale artificial cultivation apricot leaf Ke resource is imperative.Because apricot leaf Ke seed belongs to hardseed, have several factors to cause seed germination success rate low in natural germination process, the reduction of resource is not supported in the sprouting naturally of seed far away.And the apricot leaf Ke percentage of seedgermination without any hormone agents process in traditional mode of production often only has about 50%, and germinating time evening, emerge irregular, limit the large-scale planting of apricot leaf Ke.
Summary of the invention
The invention provides a kind of characteristic of Quercus acutissima and apricot leaf Ke can being combined, obtain well developed root system, growth is fast, and survival rate is high, the propagation method of apricot leaf Ke grafting Quercus acutissima container seedling of wide adaptability.
To achieve these goals, technical scheme of the present invention is as follows:
The propagation method of a kind of apricot leaf Ke grafting Quercus acutissima container seedling, comprise the operation of scion collection, grafting time, grafting and managing after grafting, Quercus acutissima container seedling carpentery workshop is selected to be stock, from the healthy and strong semi-lignified without damage by disease and insect of apricot leaf Ke elite stand clip to lignification branch as scion, cut-grafting method is adopted to carry out grafting, carry out managing after grafting, the apricot leaf Ke grafting obtained to spring next year can go out garden afforestation; Concrete operation step is as follows:
(1) scion collection: from the healthy and strong semi-lignified without damage by disease and insect of apricot leaf Ke elite stand clip to lignification branch as scion; Blade of often opening on fringe bar cuts off half, and every 50 a bundles wrapping, carries out moisturizing with wet towel or plastic foil;
(2) grafting time: the four seasons all can carry out grafting;
(3) grafting: the Quercus acutissima container seedling nursery stock that leading thread is more than 0.3cm in garden, as stock, adopts cut-grafting method to carry out grafting;
(4) managing after grafting:
Take shed after grafting and cover overlay moisturizing, and cover sunshade net to nursery stock shading, keep canopy humidity 85 ~ 90%, after young sprout is extracted in scion out, open plastic film, the rudiment in time removing stock grown; After young sprout is aging, opens sunshade net gradually, under full light, cultivates grafting; Strengthen water and fertilizer management and the extermination of disease and insect pest seedling stage, stop fertilising, make the abundant lignification of grafting after entering the winter, the apricot leaf Ke grafting obtained to spring next year can go out garden afforestation.
Preferably, above-described stock breeding method is as follows:
(1) seedbed and container prepare: select leeward on the sunny side, the place of good water permeability builds seedbed, container for plant growth is filled Nutrition Soil, is neatly placed on seedbed, sow first 1 day and with the potassium permanganate that weight concentration is 0.2%, Nutrition Soil is carried out disinfection routinely;
(2) seed treatment: select common Quercus acutissima seed with Seed soaking 1 day before sowing, then is the carbendazim solution seed dressing of 0.2% by weight concentration, and covers plastic film and carry out desinsection, and then with wet husky stored seeds, volume ratio that is husky and seed is 2:1;
(3) sow: germinate February next year to April after seed storage vernalization, the seed of germination is taken out from wet sand sowing and cultivate stock in the container for plant growth filling Nutrition Soil, each container for plant growth sows 1, and 1 ~ 2cm is thick for surface cover Nutrition Soil;
(4) young anvil management: after planting regularly trickle moisturizing, nursery stock strengthens water and fertilizer management and the extermination of disease and insect pest after being unearthed, and Quercus acutissima seedling leading thread reaches more than 0.3cm and can be used for grafting.
Preferably, the formula of above-described Nutrition Soil by volume feed is: laterite: humus soil=1 ~ 2: 1; Described container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 8 ~ 10cm × 15 ~ 20cm.
Preferably, above-described water and fertilizer management and the extermination of disease and insect pest, start fertilising after nursery stock is unearthed 20 days, based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for the 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control.
Preferably, first the grafting of above-described cut-grafting method is cutting off stock from stock base portion 10 ~ 12cm place with secateurs, vertically cut with the cross section of grafting knife at stock, the degree of depth reaches 1.5cm, then fringe bar is cut into the long scion remaining with 1 ~ 2 bud of 3 ~ 4cm, angle at 45 ° is all cut at scion two ends, angle at 45 ° is only cut in lower end by the top tip, the wound face that a 1.5cm is long is cut in the long face of scion lower end, cut the degree of depth and reach xylem, in the otch of rapid insertion stock, scion is alignd mutually with the formation layer of stock, binds with plastic film tape.
Relative to prior art, the advantage that the present invention has and good effect as follows:
1, the present invention is the once breakthrough of apricot leaf Ke vegetative propagation technique, utilizes Quercus acutissima stock to carry out propagation by grafiting, and a large amount of Fast-propagation maintains the Quercus acutissima container seedling nursery stock of maternal excellent economic characters, and seedling root is flourishing; Apricot leaf Ke Mu seedling relies on stock Quercus acutissima to the advantageous feature of the stronger adaptive capacity of the natural environment such as non-irrigated, wet, cold, and fringe bar is with stock energy quickly-healing and sprout young sprout, and the young sprout of sprouting grows rapid.
2, the present invention selects Quercus acutissima as stock, and cultivates with carrying out garden, the good characteristics such as Quercus acutissima plant strain growth is rapid, strong, and well developed root system has branch many, and rudiment power is strong, and ground, garden convenient management, simple to operate, survival rate is high.
3, cost of the present invention is low, simple to operate, expands soon numerous, reproduction coefficient is high, and apricot leaf Ke nursery stock quantity of acquisition is large, and survival rate is high, and be applicable to downgrading intensive cultivation, promote apricot leaf Ke high-quality, efficient, fast-developing to there are good economic benefit, social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1:
Select leeward on the sunny side, the place of good water permeability builds seedbed, container for plant growth is filled Nutrition Soil, is neatly placed on seedbed, sow first 1 day and with the potassium permanganate that weight concentration is 0.2%, Nutrition Soil is carried out disinfection routinely.Selecting common Quercus acutissima seed, with Seed soaking 1 day before sowing, then is the carbendazim solution seed dressing of 0.2% by weight concentration, and covers plastic film and carry out desinsection, and then with wet husky stored seeds, volume ratio that is husky and seed is 2:1.Germinate February next year to March after seed storage vernalization, the seed of germination is taken out from wet sand sowing and cultivate stock in the container for plant growth filling Nutrition Soil, each container for plant growth sows 1, and 1 ~ 2cm is thick for surface cover Nutrition Soil.Nutrition Soil by volume feed is: laterite: humus soil=1 ~ 2: 1 mix obtained.Described container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 8cm × 15cm.After planting regularly trickle moisturizing, starts fertilising after nursery stock is unearthed 20 days, and based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for the 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control.Quercus acutissima seedling leading thread reaches more than 0.3cm and can be used for grafting.
Spring or summer, from the healthy and strong semi-lignified without damage by disease and insect of apricot leaf Ke elite stand clip to lignification branch as scion; Blade of often opening on fringe bar cuts off half, and every 50 a bundles wrapping, carries out moisturizing with wet towel or plastic foil.The Quercus acutissima seedling that leading thread is more than 0.3cm in garden, as stock, adopts cut-grafting method to carry out grafting.First the grafting of cut-grafting method is cutting off stock from stock base portion 10 ~ 12cm place with secateurs, vertically cut with the cross section of grafting knife at stock, the degree of depth reaches 1.5cm, then fringe bar is cut into the long scion remaining with 1 ~ 2 bud of 3 ~ 4cm, angle at 45 ° is all cut at scion two ends, angle at 45 ° is only cut in lower end by the top tip, the wound face that a 1.5cm is long is cut in the long face of scion lower end, cut the degree of depth and reach xylem, in the otch of rapid insertion stock, scion is alignd mutually with the formation layer of stock, binds with plastic film tape.Take shed after grafting and cover overlay moisturizing, and cover sunshade net to nursery stock shading, keep canopy humidity 85 ~ 90%, after young sprout is extracted in scion out, open plastic film, the rudiment in time removing stock grown; After young sprout is aging, opens sunshade net gradually, under full light, cultivates grafting; Nursery stock is unearthed and starts fertilising after 20 days, and based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for the 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control, stops fertilising, make the abundant lignification of grafting after entering the winter, and the apricot leaf Ke grafting obtained to spring next year can go out garden afforestation.
Embodiment 2:
Select leeward on the sunny side, the place of good water permeability builds seedbed, container for plant growth is filled Nutrition Soil, is neatly placed on seedbed, sow first 1 day and with the potassium permanganate that weight concentration is 0.2%, Nutrition Soil is carried out disinfection routinely.Selecting common Quercus acutissima seed, with Seed soaking 1 day before sowing, then is the carbendazim solution seed dressing of 0.2% by weight concentration, and covers plastic film and carry out desinsection, and then with wet husky stored seeds, volume ratio that is husky and seed is 2:1.Germinate March next year to April after seed storage vernalization, the seed of germination is taken out from wet sand sowing and cultivate stock in the container for plant growth filling Nutrition Soil, each container for plant growth sows 1, and 1 ~ 2cm is thick for surface cover Nutrition Soil.Nutrition Soil by volume feed is: laterite: humus soil=2: 1 mix obtained.Described container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 10cm × 20cm.After planting regularly trickle moisturizing, starts fertilising after nursery stock is unearthed 20 days, and based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for the 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control.Quercus acutissima seedling leading thread reaches more than 0.3cm and can be used for grafting.
Autumn or winter, from the healthy and strong semi-lignified without damage by disease and insect of apricot leaf Ke elite stand clip to lignification branch as scion; Blade of often opening on fringe bar cuts off half, and every 50 a bundles wrapping, carries out moisturizing with plastic foil.The Quercus acutissima seedling that leading thread is more than 0.3cm in garden, as stock, adopts cut-grafting method to carry out grafting.First the grafting of cut-grafting method is cutting off stock from stock base portion 10 ~ 12cm place with secateurs, vertically cut with the cross section of grafting knife at stock, the degree of depth reaches 1.5cm, then fringe bar is cut into the long scion remaining with 1 ~ 2 bud of 3 ~ 4cm, angle at 45 ° is all cut at scion two ends, angle at 45 ° is only cut in lower end by the top tip, the wound face that a 1.5cm is long is cut in the long face of scion lower end, cut the degree of depth and reach xylem, in the otch of rapid insertion stock, scion is alignd mutually with the formation layer of stock, binds with plastic film tape.Take shed after grafting and cover overlay moisturizing, and cover sunshade net to nursery stock shading, keep canopy humidity 85 ~ 90%, after young sprout is extracted in scion out, open plastic film, the rudiment in time removing stock grown; After young sprout is aging, opens sunshade net gradually, under full light, cultivates grafting; Nursery stock is unearthed and starts fertilising after 20 days, and based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for the 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control, stops fertilising, make the abundant lignification of grafting after entering the winter, and the apricot leaf Ke grafting obtained to spring next year can go out garden afforestation.
Claims (5)
1. the propagation method of apricot leaf Ke grafting Quercus acutissima container seedling, it is characterized in that: the operation comprising scion collection, grafting time, grafting and managing after grafting, Quercus acutissima container seedling carpentery workshop is selected to be stock, from the healthy and strong semi-lignified without damage by disease and insect of apricot leaf Ke elite stand clip to lignification branch as scion, cut-grafting method is adopted to carry out grafting, carry out managing after grafting, the apricot leaf Ke grafting obtained to spring next year can go out garden afforestation; Concrete operation step is as follows:
(1) scion collection: from the healthy and strong semi-lignified without damage by disease and insect of apricot leaf Ke elite stand clip to lignification branch as scion; Blade of often opening on fringe bar cuts off half, and every 50 a bundles wrapping, carries out moisturizing with wet towel or plastic foil;
(2) grafting time: the four seasons all can carry out grafting;
(3) grafting: the Quercus acutissima container seedling nursery stock that leading thread is more than 0.3cm in garden, as stock, adopts cut-grafting method to carry out grafting;
(4) managing after grafting:
Take shed after grafting and cover overlay moisturizing, and cover sunshade net to nursery stock shading, keep canopy humidity 85 ~ 90%, after young sprout is extracted in scion out, open plastic film, the rudiment in time removing stock grown; After young sprout is aging, opens sunshade net gradually, under full light, cultivates grafting; Strengthen water and fertilizer management and the extermination of disease and insect pest seedling stage, stop fertilising, make the abundant lignification of grafting after entering the winter, the apricot leaf Ke grafting obtained to spring next year can go out garden afforestation.
2. the propagation method of a kind of apricot leaf Ke grafting Quercus acutissima container seedling according to claim 1, is characterized in that: described stock breeding method is as follows:
(1) seedbed and container prepare: select leeward on the sunny side, the place of good water permeability builds seedbed, container for plant growth is filled Nutrition Soil, is neatly placed on seedbed, sow first 1 day and with the potassium permanganate that weight concentration is 0.2%, Nutrition Soil is carried out disinfection routinely;
(2) seed treatment: select common Quercus acutissima seed with Seed soaking 1 day before sowing, then is the carbendazim solution seed dressing of 0.2% by weight concentration, and covers plastic film and carry out desinsection, and then with wet husky stored seeds, volume ratio that is husky and seed is 2:1;
(3) sow: germinate February next year to April after seed storage vernalization, the seed of germination is taken out from wet sand sowing and cultivate stock in the container for plant growth filling Nutrition Soil, each container for plant growth sows 1, and 1 ~ 2cm is thick for surface cover Nutrition Soil;
(4) young anvil management: after planting regularly trickle moisturizing, nursery stock strengthens water and fertilizer management and the extermination of disease and insect pest after being unearthed, and Quercus acutissima seedling leading thread reaches more than 0.3cm and can be used for grafting.
3. the propagation method of a kind of apricot leaf Ke grafting Quercus acutissima container seedling according to claim 2, is characterized in that: the formula of described Nutrition Soil by volume feed is: laterite: humus soil=1 ~ 2: 1; Described container for plant growth adopts the degradable nonwoven fabrics Seedling bag of 8 ~ 10cm × 15 ~ 20cm.
4. according to the propagation method of the arbitrary described a kind of apricot leaf Ke grafting Quercus acutissima container seedling of claim 1 or 2, it is characterized in that: described water and fertilizer management and the extermination of disease and insect pest, fertilising is started after nursery stock is unearthed 20 days, based on the aqueous solution of conventional composite fertilizer, every weeding in 15 days and fertilising 1 time, the weight concentration spraying composite fertilizer for 1st time is 0.5%, and weight concentration of applying fertilizer subsequently is increased to 1.5% gradually; Frequent trickle keeps Nutrition Soil moistening, monthly adds water 800 times with the trichlorphon original powder of weight concentration 90% and sprays pest control.
5. the propagation method of a kind of apricot leaf Ke grafting Quercus acutissima container seedling according to claim 1, it is characterized in that: first described cut-grafting method grafting is cutting off stock from stock base portion 10 ~ 12cm place with secateurs, vertically cut with the cross section of grafting knife at stock, the degree of depth reaches 1.5cm, then fringe bar is cut into the long scion remaining with 1 ~ 2 bud of 3 ~ 4cm, angle at 45 ° is all cut at scion two ends, angle at 45 ° is only cut in lower end by the top tip, the wound face that a 1.5cm is long is cut in the long face of scion lower end, cut the degree of depth and reach xylem, in the otch of rapid insertion stock, scion is alignd mutually with the formation layer of stock, bind with plastic film tape.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106416911A (en) * | 2016-10-17 | 2017-02-22 | 柳州三农科技有限公司 | Lithocarpus amygdalifolius tissue culture explant obtaining method |
| CN107258511A (en) * | 2017-06-29 | 2017-10-20 | 郴州市林业科学研究所 | Quercus acutissima bud anvil seeding cultivating method and Quercus acutissima method for culturing seedlings |
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| RU2146440C1 (en) * | 1998-11-26 | 2000-03-20 | Научно-Производственное Объединение "Виноград" | Grape graft obtaining method |
| CN103975739A (en) * | 2014-05-16 | 2014-08-13 | 广西壮族自治区林业科学研究院 | Propagation method of lithocarpus polysachyus rehd young stock grafting container-grown seedling |
| CN104303770A (en) * | 2014-10-13 | 2015-01-28 | 金华市永根杜鹃花培育有限公司 | Seedling scion grafting method |
| CN104737829A (en) * | 2015-04-10 | 2015-07-01 | 安徽省林业科学研究院 | Masson pine grafting method |
| CN104920146A (en) * | 2015-06-15 | 2015-09-23 | 广西壮族自治区林业科学研究院 | Propagation method for rapid spikelet breeding of ithocarpus polysachyus rehd |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2146440C1 (en) * | 1998-11-26 | 2000-03-20 | Научно-Производственное Объединение "Виноград" | Grape graft obtaining method |
| CN103975739A (en) * | 2014-05-16 | 2014-08-13 | 广西壮族自治区林业科学研究院 | Propagation method of lithocarpus polysachyus rehd young stock grafting container-grown seedling |
| CN104303770A (en) * | 2014-10-13 | 2015-01-28 | 金华市永根杜鹃花培育有限公司 | Seedling scion grafting method |
| CN104737829A (en) * | 2015-04-10 | 2015-07-01 | 安徽省林业科学研究院 | Masson pine grafting method |
| CN104920146A (en) * | 2015-06-15 | 2015-09-23 | 广西壮族自治区林业科学研究院 | Propagation method for rapid spikelet breeding of ithocarpus polysachyus rehd |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106416911A (en) * | 2016-10-17 | 2017-02-22 | 柳州三农科技有限公司 | Lithocarpus amygdalifolius tissue culture explant obtaining method |
| CN107258511A (en) * | 2017-06-29 | 2017-10-20 | 郴州市林业科学研究所 | Quercus acutissima bud anvil seeding cultivating method and Quercus acutissima method for culturing seedlings |
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