CN105219823A - 一种酶法制备谷胱甘肽的方法 - Google Patents
一种酶法制备谷胱甘肽的方法 Download PDFInfo
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Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
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Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
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WO2018228246A1 (zh) * | 2017-06-15 | 2018-12-20 | 安徽古特生物科技有限公司 | 一种酶法制备谷胱甘肽的方法 |
WO2018228247A1 (zh) * | 2017-06-15 | 2018-12-20 | 安徽古特生物科技有限公司 | 一种利用腺苷代替atp进行酶促反应的生产方法 |
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CN110343651A (zh) * | 2019-06-11 | 2019-10-18 | 张家港市华天药业有限公司 | 一种用于合成谷胱甘肽的重组菌及其制备方法 |
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CN112779173A (zh) * | 2021-01-06 | 2021-05-11 | 江南大学 | 一种高产谷胱甘肽毕赤酵母菌株g3-sf及其应用 |
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-
2015
- 2015-11-10 CN CN201510762184.XA patent/CN105219823B/zh active Active
Non-Patent Citations (1)
Title |
---|
陈阳: "偶联ATP再生用于谷胱甘肽的酶法合成", 《2013中国化工学会年会论文集》 * |
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