CN105198967B - 蛋白质VdAL在提高植物产品品质中的应用 - Google Patents
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- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
标签 | 残基 | 序列 |
Poly-Arg | 5-6(通常为5个) | RRRRR |
Poly-His | 2-10(通常为6个) | HHHHHH |
FLAG | 8 | DYKDDDDK |
Strep-tag II | 8 | WSHPQFEK |
c-myc | 10 | EQKLISEEDL |
指标 | 未处理 | VdLA处理 |
含水量(%) | 90.22 | 91.43 |
干物率(%) | 9.78 | 8.57 |
Vc(mg/100g) | 12.1 | 18.2 |
可溶性糖(%) | 6.83 | 7.44 |
可滴定酸(%) | 0.09 | 0.09 |
可溶性固形物(%) | 9.1 | 9.1 |
瓜皮厚(cm) | 0.97 | 1.23 |
蛋白质(%DW) | 5.54 | 6.33 |
蛋白质(%FW) | 0.54 | 0.54 |
粗纤维(%DW) | 2.06 | 2.02 |
粗纤维(%FW) | 0.2 | 0.17 |
编号 | 指标 | 未处理 | VdAL处理 |
1 | 含水率(%) | 90.22 | 91.43 |
2 | Ca(mg/L) | 20.13 | 22.70 |
3 | Ca含量(mg/100gFw) | 9.83 | 9.71 |
4 | Cu(mg/L) | 0.13 | 0.15 |
5 | Cu含量(mg/100gFw) | 0.06 | 0.06 |
6 | Fe(mg/L) | 0.50 | 0.60 |
7 | Fe含量(mg/100gFw) | 2.50 | 2.98 |
8 | K(mg/L) | 158.00 | 184.00 |
9 | K含量(mg/100gFw) | 77.19 | 78.64 |
10 | Mg(mg/L) | 22.27 | 22.50 |
11 | Mg含量(mg/100gFw) | 10.88 | 9.63 |
12 | Mn(mg/L) | 0.10 | 0.12 |
13 | Mn含量(mg/100gFw) | 0.05 | 0.05 |
14 | Na(mg/L) | 7.20 | 7.35 |
15 | Na含量(mg/100gFw) | 3.52 | 3.14 |
16 | P(mg/L) | 18.30 | 20.13 |
17 | P含量(mg/100gFw) | 8.93 | 8.65 |
18 | Zn(mg/L) | 0.25 | 0.30 |
19 | Zn含量(mg/100gFw) | 0.12 | 0.13 |
Claims (11)
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
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CN201510691295.6A CN105198967B (zh) | 2015-10-22 | 2015-10-22 | 蛋白质VdAL在提高植物产品品质中的应用 |
CA3002881A CA3002881A1 (en) | 2015-10-22 | 2016-07-01 | Applications of protein vdal in improving output, product quality and drought resistance of plant and in improving fruit coloring of plant |
PCT/CN2016/088074 WO2017067214A1 (zh) | 2015-10-22 | 2016-07-01 | 蛋白质VdAL在提高植物产量、产品品质和抗旱性及促进植物果实着色中的应用 |
US15/769,746 US10806145B2 (en) | 2015-10-22 | 2016-07-01 | Applications of protein VdAL in improving output, product quality and drought resistance of plant and in improving fruit coloring of plant |
EP16856654.5A EP3351555A4 (en) | 2015-10-22 | 2016-07-01 | APPLICATIONS OF PROTEIN-VDAL TO IMPROVE PERFORMANCE, PRODUCT QUALITY AND RESISTANCE TO DRY PLANT HEALTH AND IMPROVED FRUIT COLORATION OF PLANTS |
CL2018001026A CL2018001026A1 (es) | 2015-10-22 | 2018-04-20 | Aplicaciones de la proteína vdal en el mejoramiento de el rendimiento, calidad del producto y resistencia a la sequía de plantas y en la promoción de la coloración de los frutos de la planta |
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CN201510691295.6A CN105198967B (zh) | 2015-10-22 | 2015-10-22 | 蛋白质VdAL在提高植物产品品质中的应用 |
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CN104558130B (zh) | 2015-01-06 | 2017-12-15 | 中国农业大学 | 抗病相关蛋白及其编码基因与它们在调控植物抗病性中的应用 |
EP3351555A4 (en) * | 2015-10-22 | 2019-08-21 | Pherobio Technology Co., Ltd | APPLICATIONS OF PROTEIN-VDAL TO IMPROVE PERFORMANCE, PRODUCT QUALITY AND RESISTANCE TO DRY PLANT HEALTH AND IMPROVED FRUIT COLORATION OF PLANTS |
CN115413678A (zh) * | 2022-09-20 | 2022-12-02 | 中捷四方生物科技股份有限公司 | 一种含大丽轮枝孢激活蛋白的可湿性粉剂和应用 |
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CN104558130A (zh) * | 2015-01-06 | 2015-04-29 | 中国农业大学 | 抗病相关蛋白及其编码基因与它们在调控植物抗病性中的应用 |
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CN104558130A (zh) * | 2015-01-06 | 2015-04-29 | 中国农业大学 | 抗病相关蛋白及其编码基因与它们在调控植物抗病性中的应用 |
Non-Patent Citations (4)
Title |
---|
allergen Asp f2-like protein [Verticillium dahliae],Accession No: AY524789.1;Wang,J. Y. et al;《Genbank数据库》;20060130;全文 |
NPP1,a phytophthora-associated trigger of plant defense in parsley and Arabidopsis;Guido Fellbrich et al.;《The plant jouranl》;20021231;第32卷;第375-390页 |
Purification of protein from culture filtrates of Fusarium oxysporum that induces ethylene and necrosis in leaves of Erythroxylum coca;Bryan A. Bailey;《Phytopatholgogy》;19951231;第85卷(第10期);第1250-1255页 |
VdNEP,an elicitor from Verticillium dahliae,induces cotton plant wilting;Jian-Ying Wang et al.;《Applied and environmental microbiology》;20040831;第70卷(第8期);摘要,第4990页左栏第5段,右栏第8段,第4992页左栏第2-3段,第4994页左栏第3段,图3D |
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