CN105198508B - A kind of preparation method and application method of bamboo grove organic fertilizer - Google Patents
A kind of preparation method and application method of bamboo grove organic fertilizer Download PDFInfo
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- CN105198508B CN105198508B CN201510727644.5A CN201510727644A CN105198508B CN 105198508 B CN105198508 B CN 105198508B CN 201510727644 A CN201510727644 A CN 201510727644A CN 105198508 B CN105198508 B CN 105198508B
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Abstract
The present invention provides a kind of preparation methods of bamboo grove organic fertilizer, this method is by selecting bamboo grove dry branches and fallen leaves to obtain nutrient solution, by the process of strain separating, purifying, culture, breeding, obtain the dominant bacteria of bamboo grove litter, the dominant bacteria of acquisition is inoculated into the compost of bamboo grove dry branches and fallen leaves production and is fermented, and then obtains bamboo grove organic fertilizer.Bamboo grove organic fertilizer prepared by the method for this offer can change the physical structure of soil, improvement soil hardening, retain water and nutrients, increase soil fertility, increases quantity of useful microbe in soil, murder soil nocuousness miscellaneous bacteria according to the present invention, and then the growth of promotion bamboo class root system.Invention additionally provides the application method of bamboo grove organic fertilizer, and the application method of the bamboo grove organic fertilizer provided through the invention can make bamboo grove hair bamboo shoot rate improve 27.5%, improves 10% at bamboo rate, Growth of young bamboo speed improves 15%.
Description
Technical field
The present invention relates to the technical fields of organic fertilizer preparation, more specifically, are related to a kind of bamboo grove organic fertilizer
Preparation method and application method.
Background technique
Bamboo generic gramineae plant is the important forest plants in the areas such as China torrid zone and subtropical zone.Bamboo class is due to having
Special growth vigor and important economic value and the attention increasingly by countries in the world.Simultaneously because bamboo class is with higher
Ornamental value, thus the cultivation of bamboo grove also increasingly attracts people's attention.The existing bamboo grove area about 7,200,000 in China according to statistics
Hectare, wherein the area for manually managing bamboo grove reaches 4,200,000 hectares, this illustrates the quality of artificial culture bamboo class situation by direct shadow
The rate of growth of bamboo class, rate of growing into forest are rung, and then influences the ornamental value of bamboo class.Therefore, in manually culture bamboo grove, how bamboo is improved
Hair bamboo shoot rate, rate of growth and the rate of growing into forest of woods become the problem that Garden Greening Work person faces.
During bamboo class sends out bamboo shoot and growth, the application of fertilizer plays an important role.In general, bamboo class hair bamboo shoot and life
The fertilizer applied in long process is chemical fertilizer.Chemical fertilizer is easy to use, and fertilizer efficiency is fast, thus by universal application.
But chemical fertilizer needs to consume mass energy in production, and then improves the high production cost of chemical fertilizer, meanwhile, change
Learning fertilizer all can generate pollution to water and atmosphere in production and use, cause fertilizer to be largely lost, and then make plant utilization rate
It reduces.At the same time, chemical fertilizer element is single, is difficult to rational proportion when collocation mutually, so that hair bamboo shoot are reduced, even if bamboo class is sent out
Bamboo shoot can not grow up to healthy and strong bamboo.
Summary of the invention
The object of the present invention is to provide the preparation methods and application method of a kind of bamboo grove organic fertilizer, to solve background technique
Described can make the problem of sending out bamboo shoot reduction using chemical fertilizer.
In order to solve the above technical problem, the present invention provides following technical solutions:
The present invention provides a kind of preparation method of bamboo grove organic fertilizer, the preparation method includes:
S01: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S02: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S03: the bamboo grove dry branches and fallen leaves being cut into small pieces is inoculated into improvement potato dextrose agar plate culture medium (improvement
PDA plate culture medium) in, it is cultivated under the conditions of temperature is 22-26 DEG C, until growing bacterium colony；
S04: dominant colony is chosen, the mycelia at dominant colony edge is transferred to new improvement potato dextrose agar and is put down
Continue to cultivate in plate culture medium, until growing pure mycelia；
S05: choosing eugonic pure mycelia, by pure mycelium inoculation to improvement potato dextrose agar fluid nutrient medium
It is cultivated in (improvement PDA liquid medium), obtains liquid bacterial agent；
S06: aforesaid liquid microbial inoculum is inoculated into and continues to cultivate in solid culture medium, obtains solid fungicide；
S07: above-mentioned solid fungicide being inoculated into and is expanded in compost, and is alternately put using solid fungicide and expansion compost
The mode set is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer.
Preferably, the production method of the improvement PDA plate culture medium are as follows: boil bamboo grove dry branches and fallen leaves and horse respectively with boiling water
Each 20-30min of bell potato, and bamboo grove dry branches and fallen leaves water cooking liquid and 150-200g/L that concentration is 150-200g/L are obtained by filtration respectively
Potato fruit；The bamboo grove dry branches and fallen leaves water cooking liquid being obtained by filtration, water and potato fruit are mixed, mixed liquor is obtained；To
Agar is added in mixed liquor, and heats while stirring and melts completely to agar, agar adds glucose after melting completely, and stirs
Uniformly；Using concentration to adjust pH value for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly is 5-6, and is gone out
Bacterium processing；It is cooling after sterilizing to obtain improvement PDA plate culture medium.
Preferably, the volume ratio of the bamboo grove dry branches and fallen leaves water cooking liquid, water and potato fruit is 1-2:4-6:3-4.
Preferably, the mass ratio of the potato, glucose and agar is 15-20:1-2:1-2.
Preferably, it includes: every for pure mycelium inoculation being carried out culture into improvement PDA liquid medium described in step S05
It rises the culture solution in improvement PDA liquid medium and is inoculated with the pure mycelia that 30-50 diameter is 6-9mm, and be 22-28 in temperature
DEG C, revolving speed be 120-150r/min under conditions of cultivate 12-15 days.
Preferably, aforesaid liquid microbial inoculum is inoculated into solid culture medium described in step S06 continue culture include: every thousand
Gram solid culture medium is inoculated with 400-600ml liquid bacterial agent, and continues to cultivate liquid bacterial agent under conditions of temperature is 22-28 DEG C.
Preferably, the solid culture medium includes the bamboo grove dry branches and fallen leaves and corn flour that mass ratio is 6-8:2-4.
Preferably, above-mentioned solid fungicide is inoculated into expansion compost described in step S07 includes: every kilogram of expansion training
100-200g solid fungicide is inoculated in nutriment.
Preferably, the compost that expands includes the bamboo grove dry branches and fallen leaves and trees dry branches and fallen leaves that mass ratio is 7-8:2-3,
And the water content for expanding compost is 45-60%.
The present invention also provides a kind of application method of bamboo grove organic fertilizer, which includes:
It applies fertilizer in January December to next year；Scattered bamboos apply bamboo grove organic fertilizer 40- using the method cultivated again is spread fertilizer over the fields per acre
50kg, chemical fertilizer 20-25kg；The method that Cluster Bamboo is applied using the outer cave of pocket, every clump of application bamboo grove organic fertilizer 1-2kg, chemistry
Fertilizer 1-2kg.
The preparation method of bamboo grove organic fertilizer provided by the invention selects bamboo grove dry branches and fallen leaves to obtain nutrient solution, by strain
The process of separation, purifying, culture, breeding obtains the dominant bacteria of bamboo grove litter, the dominant bacteria of acquisition is inoculated into
It ferments in the compost of bamboo grove dry branches and fallen leaves production, and then obtains bamboo grove organic fertilizer.Bamboo grove provided by the present invention has
Machine fertilizer, which is added in soil, can reach the physical structure for changing soil, improvement soil hardening, retain water and nutrients, improve soil fertilizer
The effect of power, and then promote the growth of bamboo class root system.In addition, bamboo grove organic fertilizer provided by the present invention is added in soil also
The quantity of useful microbe in soil can be increased, murder soil nocuousness miscellaneous bacteria, and greatly reduce the application of chemical fertilizer.This hair
The preparation method of bamboo grove organic fertilizer provided by bright is easy to operate, easily controllable, can be used in being mass produced.The present invention is also
The application method of bamboo grove organic fertilizer is provided, the application method of the bamboo grove organic fertilizer provided through the invention can make bamboo grove
Send out bamboo shoot time advance, improve the survival rate of bamboo shoots, accelerate the speed of growth of young bamboo, increase bamboo grove at bamboo rate.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, for those of ordinary skills, do not making the creative labor
Under the premise of, it can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the flow diagram of the preparation method of bamboo grove organic fertilizer provided in an embodiment of the present invention.
Specific embodiment
The preparation method and application method of bamboo grove organic fertilizer provided in an embodiment of the present invention, solve using chemical fertilizer
It can make to send out the problem of bamboo shoot are reduced.
Technical solution in embodiment in order to enable those skilled in the art to better understand the present invention, and make of the invention real
The above objects, features, and advantages for applying example can be more obvious and easy to understand, with reference to the accompanying drawing to the technology in the embodiment of the present invention
Scheme is described in further detail.
Attached drawing 1 is please referred to, the process that attached drawing 1 shows bamboo grove organic fertilizer preparation method provided in an embodiment of the present invention is shown
It is intended to.In embodiment provided by the invention, improvement PDA liquid medium is not add fine jade in improvement PDA plate culture medium
Rouge, and remaining condition it is constant on the basis of be prepared.Trees dry branches and fallen leaves in the present invention is Poplar leaves, Folium Pterocaryae, Chinese scholartree
One of dry branches and fallen leaves of tree species such as leaf and phoenix tree leaf is a variety of.The present invention does not carry out the specific type of trees dry branches and fallen leaves
Limitation.
Embodiment 1
S101: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S102: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S103: the bamboo grove dry branches and fallen leaves being cut into small pieces is inoculated into improvement PDA plate culture medium, is 22 DEG C of items in temperature
It is cultivated under part, until growing bacterium colony；
S104: choosing dominant colony, and the mycelia at dominant colony edge is transferred to new improvement PDA plate culture medium and is relayed
Continuous culture, until growing pure mycelia；
S105: choosing eugonic pure mycelia, and pure mycelium inoculation is cultivated into improvement PDA liquid medium,
Obtain liquid bacterial agent；
S106: aforesaid liquid microbial inoculum is inoculated into and continues to cultivate in solid culture medium, obtains solid fungicide；
S107: above-mentioned solid fungicide being inoculated into and is expanded in compost, and using solid fungicide and expands compost alternating
The mode of placement is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 40kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 20kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 1kg；Chemical fertilizer 1kg.
Embodiment 2
S201: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S202: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S203: the bamboo grove dry branches and fallen leaves being cut into small pieces is inoculated into improvement PDA plate culture medium, is 26 DEG C of items in temperature
It is cultivated under part, until growing bacterium colony；
S204: choosing dominant colony, and the mycelia at dominant colony edge is transferred to new improvement PDA plate culture medium and is relayed
Continuous culture, until growing pure mycelia；
S205: choosing eugonic pure mycelia, and pure mycelium inoculation is cultivated into improvement PDA liquid medium,
Obtain liquid bacterial agent；
S206: aforesaid liquid microbial inoculum is inoculated into and continues to cultivate in solid culture medium, obtains solid fungicide；
S207: above-mentioned solid fungicide being inoculated into and is expanded in compost, and using solid fungicide and expands compost alternating
The mode of placement is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 50kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 25kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 2kg；Chemical fertilizer 2kg.
Embodiment 3
S301: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S302: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S303: boiling bamboo grove dry branches and fallen leaves and each 20min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 150g/L and the potato fruit of 150g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 1:4:3 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly, wherein potato, glucose and
The mass ratio of agar is 15:1:1；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 5, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S304: choosing dominant colony, and the mycelia at dominant colony edge is transferred to new improvement PDA plate culture medium and is relayed
Continuous culture, until growing pure mycelia；
S305: choosing eugonic pure mycelia, and pure mycelium inoculation is cultivated into improvement PDA liquid medium,
Obtain liquid bacterial agent；
S306: aforesaid liquid microbial inoculum is inoculated into and continues to cultivate in solid culture medium, obtains solid fungicide；
S307: above-mentioned solid fungicide being inoculated into and is expanded in compost, and using solid fungicide and expands compost alternating
The mode of placement is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 40kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 20kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 1kg；Chemical fertilizer 1kg.
Embodiment 4
S401: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S402: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S403: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 2:6:4 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 20:2:2；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S404: choosing dominant colony, and the mycelia at dominant colony edge is transferred to new improvement PDA plate culture medium and is relayed
Continuous culture, until growing pure mycelia；
S405: choosing eugonic pure mycelia, and pure mycelium inoculation is cultivated into improvement PDA liquid medium,
Obtain liquid bacterial agent；
S406: aforesaid liquid microbial inoculum is inoculated into and continues to cultivate in solid culture medium, obtains solid fungicide；
S407: above-mentioned solid fungicide being inoculated into and is expanded in compost, and using solid fungicide and expands compost alternating
The mode of placement is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 50kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 25kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 2kg；Chemical fertilizer 2kg.
Embodiment 5
S501: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S502: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S503: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 1:6:4 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 15:2:2；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S504: choosing dominant colony, and the mycelia at dominant colony edge is transferred to new improvement PDA plate culture medium and is relayed
Continuous culture, until growing pure mycelia；
S505: choosing eugonic pure mycelia, and the culture solution in every liter of improvement PDA liquid medium is inoculated with 30 diameters
It for the pure mycelia of 6mm, and is cultivated 12 days under conditions of temperature is 22 DEG C, revolving speed is 120r/min, obtains liquid bacterial agent；
S506: aforesaid liquid microbial inoculum is inoculated into and continues to cultivate in solid culture medium, obtains solid fungicide；
S507: above-mentioned solid fungicide being inoculated into and is expanded in compost, and using solid fungicide and expands compost alternating
The mode of placement is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 40kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 20kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 1kg；Chemical fertilizer 1kg.
Embodiment 6
S601: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S602: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S603: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 2:5:3 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 20:1:1；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S604: choosing dominant colony, and the mycelia at dominant colony edge is transferred to new improvement PDA plate culture medium and is relayed
Continuous culture, until growing pure mycelia；
S605: choosing eugonic pure mycelia, and the culture solution in every liter of improvement PDA liquid medium is inoculated with 50 diameters
It for the pure mycelia of 9mm, and is cultivated 15 days under conditions of temperature is 28 DEG C, revolving speed is 150r/min, obtains liquid bacterial agent；
S606: aforesaid liquid microbial inoculum is inoculated into and continues to cultivate in solid culture medium, obtains solid fungicide；
S607: above-mentioned solid fungicide being inoculated into and is expanded in compost, and using solid fungicide and expands compost alternating
The mode of placement is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 50kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 25kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 2kg；Chemical fertilizer 2kg.
Embodiment 7
S701: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S702: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S703: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 1:6:3 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 15:2:2；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S704: choosing dominant colony, and the mycelia at dominant colony edge is transferred to new improvement PDA plate culture medium and is relayed
Continuous culture, until growing pure mycelia；
S705: choosing eugonic pure mycelia, and the culture solution in every liter of improvement PDA liquid medium is inoculated with 50 diameters
It for the pure mycelia of 9mm, and is cultivated 15 days under conditions of temperature is 28 DEG C, revolving speed is 150r/min, obtains liquid bacterial agent；
S706: every kilogram above-mentioned solid culture medium is inoculated with 400ml liquid bacterial agent, and continues under conditions of temperature is 22 DEG C
Liquid bacterial agent is cultivated, solid fungicide is obtained；
S707: above-mentioned solid fungicide being inoculated into and is expanded in compost, and using solid fungicide and expands compost alternating
The mode of placement is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 40kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 20kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 1kg；Chemical fertilizer 1kg.
Embodiment 8
S801: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S802: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S803: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 2:6:4 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 20:2:2；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S804: choosing dominant colony, and the mycelia at dominant colony edge is transferred to new improvement PDA plate culture medium and is relayed
Continuous culture, until growing pure mycelia；
S805: choosing eugonic pure mycelia, and the culture solution in every liter of improvement PDA liquid medium is inoculated with 50 diameters
It for the pure mycelia of 9mm, and is cultivated 15 days under conditions of temperature is 28 DEG C, revolving speed is 150r/min, obtains liquid bacterial agent；
S806: every kilogram above-mentioned solid culture medium is inoculated with 600ml liquid bacterial agent, and continues under conditions of temperature is 28 DEG C
Liquid bacterial agent is cultivated, solid fungicide is obtained；
S807: above-mentioned solid fungicide being inoculated into and is expanded in compost, and using solid fungicide and expands compost alternating
The mode of placement is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 50kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 25kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 2kg；Chemical fertilizer 2kg.
Embodiment 9
S901: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S902: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S903: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 2:6:4 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 20:2:2；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S904: choosing dominant colony, and the mycelia at dominant colony edge is transferred to new improvement PDA plate culture medium and is relayed
Continuous culture, until growing pure mycelia；
S905: choosing eugonic pure mycelia, and the culture solution in every liter of improvement PDA liquid medium is inoculated with 50 diameters
It for the pure mycelia of 9mm, and is cultivated 15 days under conditions of temperature is 28 DEG C, revolving speed is 150r/min, obtains liquid bacterial agent；
S906: bamboo grove dry branches and fallen leaves and corn flour being mixed according to mass ratio for 6:2, solid culture medium is obtained, will
Every kilogram of above-mentioned solid culture medium is inoculated with 600ml liquid bacterial agent, and continues to cultivate liquid bacterial agent under conditions of temperature is 28 DEG C,
Obtain solid fungicide；
S907: above-mentioned solid fungicide being inoculated into and is expanded in compost, and using solid fungicide and expands compost alternating
The mode of placement is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 40kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 20kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 1kg；Chemical fertilizer 1kg.
Embodiment 10
S1001: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S1002: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S1003: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 2:6:4 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 20:2:2；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S1004: dominant colony is chosen, the mycelia at dominant colony edge is transferred in new improvement PDA plate culture medium
Continue to cultivate, until growing pure mycelia；
S1005: choosing eugonic pure mycelia, and the culture solution in every liter of improvement PDA liquid medium is inoculated with 50 directly
Diameter is the pure mycelia of 9mm, and cultivates 15 days under conditions of temperature is 28 DEG C, revolving speed is 150r/min, obtains liquid bacterial agent；
S1006: bamboo grove dry branches and fallen leaves and corn flour being mixed according to mass ratio for 8:4, solid culture medium is obtained, will
Every kilogram of above-mentioned solid culture medium is inoculated with 600ml liquid bacterial agent, and continues to cultivate liquid bacterial agent under conditions of temperature is 28 DEG C,
Obtain solid fungicide；
S1007: above-mentioned solid fungicide being inoculated into and is expanded in compost, and using solid fungicide and expands compost alternating
The mode of placement is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 50kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 25kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 2kg；Chemical fertilizer 2kg.
Embodiment 11
S1101: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S1102: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S1103: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 2:6:4 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 20:2:2；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S1104: dominant colony is chosen, the mycelia at dominant colony edge is transferred in new improvement PDA plate culture medium
Continue to cultivate, until growing pure mycelia；
S1105: choosing eugonic pure mycelia, and the culture solution in every liter of improvement PDA liquid medium is inoculated with 50 directly
Diameter is the pure mycelia of 9mm, and cultivates 15 days under conditions of temperature is 28 DEG C, revolving speed is 150r/min, obtains liquid bacterial agent；
S1106: bamboo grove dry branches and fallen leaves and corn flour being mixed according to mass ratio for 7:3, solid culture medium is obtained, will
Every kilogram of above-mentioned solid culture medium is inoculated with 600ml liquid bacterial agent, and continues to cultivate liquid bacterial agent under conditions of temperature is 28 DEG C,
Obtain solid fungicide；
S1107: every kilogram expands inoculation 100g solid fungicide in compost, and the water content for expanding compost is
45%, and placed in such a way that solid fungicide and expansion compost are alternately placed, fermentation to expansion compost is decomposed
Completely, bamboo grove organic fertilizer is obtained.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 40kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 20kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 1kg；Chemical fertilizer 1kg.
Embodiment 12
S1201: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S1202: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S1203: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 2:6:4 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 20:2:2；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S1204: dominant colony is chosen, the mycelia at dominant colony edge is transferred in new improvement PDA plate culture medium
Continue to cultivate, until growing pure mycelia；
S1205: choosing eugonic pure mycelia, and the culture solution in every liter of improvement PDA liquid medium is inoculated with 50 directly
Diameter is the pure mycelia of 9mm, and cultivates 15 days under conditions of temperature is 28 DEG C, revolving speed is 150r/min, obtains liquid bacterial agent；
S1206: bamboo grove dry branches and fallen leaves and corn flour being mixed according to mass ratio for 6:4, solid culture medium is obtained, will
Every kilogram of above-mentioned solid culture medium is inoculated with 600ml liquid bacterial agent, and continues to cultivate liquid bacterial agent under conditions of temperature is 28 DEG C,
Obtain solid fungicide；
S1207: every kilogram expands inoculation 200g solid fungicide in compost, and the water content for expanding compost is
60%, and placed in such a way that solid fungicide and expansion compost are alternately placed, fermentation to expansion compost is decomposed
Completely, bamboo grove organic fertilizer is obtained.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 50kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 25kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 2kg；Chemical fertilizer 2kg.
Embodiment 13
S1301: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S1302: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S1303: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 2:6:4 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 20:2:2；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S1304: dominant colony is chosen, the mycelia at dominant colony edge is transferred in new improvement PDA plate culture medium
Continue to cultivate, until growing pure mycelia；
S1305: choosing eugonic pure mycelia, and the culture solution in every liter of improvement PDA liquid medium is inoculated with 50 directly
Diameter is the pure mycelia of 9mm, and cultivates 15 days under conditions of temperature is 28 DEG C, revolving speed is 150r/min, obtains liquid bacterial agent；
S1306: bamboo grove dry branches and fallen leaves and corn flour being mixed according to mass ratio for 8:2, solid culture medium is obtained, will
Every kilogram of above-mentioned solid culture medium is inoculated with 600ml liquid bacterial agent, and continues to cultivate liquid bacterial agent under conditions of temperature is 28 DEG C,
Obtain solid fungicide；
S1307: bamboo grove dry branches and fallen leaves and trees dry branches and fallen leaves are mixed according to mass ratio for 7:2, obtain expanding training
Nutriment, and the water content for expanding compost is made to be 45%, 100g solid fungicide will be inoculated in every kilogram of expansion compost,
And placed in such a way that solid fungicide and expansion compost are alternately placed, fermentation to expansion compost is decomposed completely,
Obtain bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 40kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 20kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 1kg；Chemical fertilizer 1kg.
Embodiment 14
S1401: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S1402: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S1403: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 2:6:4 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 20:2:2；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S1404: dominant colony is chosen, the mycelia at dominant colony edge is transferred in new improvement PDA plate culture medium
Continue to cultivate, until growing pure mycelia；
S1405: choosing eugonic pure mycelia, and the culture solution in every liter of improvement PDA liquid medium is inoculated with 50 directly
Diameter is the pure mycelia of 9mm, and cultivates 15 days under conditions of temperature is 28 DEG C, revolving speed is 150r/min, obtains liquid bacterial agent；
S1406: bamboo grove dry branches and fallen leaves and corn flour being mixed according to mass ratio for 8:2, solid culture medium is obtained, will
Every kilogram of above-mentioned solid culture medium is inoculated with 600ml liquid bacterial agent, and continues to cultivate liquid bacterial agent under conditions of temperature is 28 DEG C,
Obtain solid fungicide；
S1407: bamboo grove dry branches and fallen leaves and trees dry branches and fallen leaves are mixed according to mass ratio for 8:3, obtain expanding training
Nutriment, and the water content for expanding compost is made to be 60%, 200g solid fungicide will be inoculated in every kilogram of expansion compost,
And placed in such a way that solid fungicide and expansion compost are alternately placed, fermentation to expansion compost is decomposed completely,
Obtain bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 50kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 25kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 2kg；Chemical fertilizer 2kg.
Embodiment 15
S1501: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S1502: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S1503: boiling bamboo grove dry branches and fallen leaves and each 30min of potato with boiling water respectively, and concentration is obtained by filtration respectively and is
The bamboo grove dry branches and fallen leaves water cooking liquid of 200g/L and the potato fruit of 200g/L；The bamboo grove dry branches and fallen leaves boiling that will be obtained by filtration
Liquid, water and potato fruit are 2:6:4 mixing according to volume ratio, obtain mixed liquor；Agar is added into mixed liquor, and side is heated
Melt completely while stirring to agar, glucose is added in agar after melting completely, and stirs evenly；Wherein, potato, glucose and
The mass ratio of agar is 20:2:2；Concentration is used to adjust pH for the NaOH of the HCl of 0.1mol/L and 0.1mol/L after mixing evenly
Value is 6, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement PDA plate culture medium.The bamboo grove dry branches and fallen leaves that will be cut into small pieces
It is inoculated into improvement PDA plate culture medium, is cultivated under the conditions of temperature is 26 DEG C, until growing bacterium colony；
S1504: dominant colony is chosen, the mycelia at dominant colony edge is transferred in new improvement PDA plate culture medium
Continue to cultivate, until growing pure mycelia；
S1505: choosing eugonic pure mycelia, and the culture solution in every liter of improvement PDA liquid medium is inoculated with 50 directly
Diameter is the pure mycelia of 9mm, and cultivates 15 days under conditions of temperature is 28 DEG C, revolving speed is 150r/min, obtains liquid bacterial agent；
S1506: bamboo grove dry branches and fallen leaves and corn flour being mixed according to mass ratio for 8:2, solid culture medium is obtained, will
Every kilogram of above-mentioned solid culture medium is inoculated with 600ml liquid bacterial agent, and continues to cultivate liquid bacterial agent under conditions of temperature is 28 DEG C,
Obtain solid fungicide；
S1507: bamboo grove dry branches and fallen leaves and trees dry branches and fallen leaves are mixed according to mass ratio for 8:2, obtain expanding training
Nutriment, and the water content for expanding compost is made to be 60%, 200g solid fungicide will be inoculated in every kilogram of expansion compost,
And placed in such a way that solid fungicide and expansion compost are alternately placed, fermentation to expansion compost is decomposed completely,
Obtain bamboo grove organic fertilizer.
The present invention also provides the application method of bamboo grove organic fertilizer, this method is to apply fertilizer in January December to next year；It is scattered
Bamboo applies bamboo grove organic fertilizer 50kg using the method cultivated again is spread fertilizer over the fields per acre；Chemical fertilizer 25kg；Cluster Bamboo is applied using the outer cave of pocket
Method, every clump of application bamboo grove organic fertilizer 2kg；Chemical fertilizer 2kg.
Test is compared using bamboo grove organic fertilizer and conventional chemical fertilizers prepared by embodiment 15, tests bamboo grove
Shooting quantity, Growth of young bamboo rate and rate of growth.Comparative test content is as follows:
1, it chooses to bamboo grove
Test bamboo grove: yellow stalk crow feeds chicken bamboo 667m2；
Compare bamboo grove: yellow stalk crow feeds chicken bamboo 667m2。
2, using fertilizer and quality
It tests bamboo grove: using bamboo grove organic fertilizer 30kg, chemical fertilizer 10kg per acre；
It compares bamboo grove: using chemical fertilizer 40kg per acre.
Bamboo grove and control bamboo grove are tested according to above-mentioned application fertilizer mode in triplicate, and test result is listed in Table 1 below.
Table 1: test result
By table 1, it can be seen that the shooting quantity of test bamboo grove, growth rate and the hair bamboo shoot for being higher than control bamboo grove at bamboo rate
Quantity, growth rate and at bamboo rate, and can be learnt by calculating, test the hair bamboo shoot rate in forest land, young bamboo rate of growth and at bamboo rate with
It compares the hair bamboo shoot rate of bamboo grove, young bamboo rate of growth and is respectively increased 27.5%, 15% and 10% with comparing at bamboo rate.Meanwhile testing bamboo
The hair bamboo shoot time of woods shifts to an earlier date 3-5 days than compareing the hair bamboo shoot time of bamboo grove.It is possible thereby to illustrate, by preparation provided by the present invention
Bamboo grove organic fertilizer prepared by method can effectively make bamboo grove send out bamboo shoot time advance, improve the survival rate of bamboo shoots, accelerate children
The speed of growth of bamboo, increase bamboo grove at bamboo rate, while can also change soil physical structure, improvement soil hardening, water conservation protect
Fertilizer increases soil fertility, increases quantity of useful microbe in soil, murders soil nocuousness miscellaneous bacteria, and then reaches promotion bamboo class
The purpose of root growth.
The embodiments of the present invention described above are not intended to limit the scope of the present invention.It is any in the present invention
Spirit and principle within made modifications, equivalent substitutions and improvements etc., should all be included in the protection scope of the present invention.
Claims (9)
1. the preparation method of bamboo grove organic fertilizer, which is characterized in that the preparation method includes:
S01: acquisition has the bamboo grove dry branches and fallen leaves of fungi in the litter of healthy bamboo grove；
S02: collected bamboo grove dry branches and fallen leaves is cut into small pieces；
S03: the bamboo grove dry branches and fallen leaves being cut into small pieces is inoculated into improvement potato dextrose agar plate culture medium, in temperature
It is cultivated under the conditions of being 22-26 DEG C, until growing bacterium colony；
S04: choosing dominant colony, and the mycelia at dominant colony edge is transferred to new improvement potato dextrose agar plate and is trained
It supports and continues to cultivate in base, until growing pure mycelia；
S05: choosing eugonic pure mycelia, by pure mycelium inoculation into improvement potato dextrose agar fluid nutrient medium into
Row culture, obtains liquid bacterial agent；
S06: aforesaid liquid microbial inoculum is inoculated into and continues to cultivate in solid culture medium, obtains solid fungicide；
S07: above-mentioned solid fungicide being inoculated into and is expanded in compost, and using solid fungicide and expands what compost was alternately placed
Mode is placed, and fermentation is decomposed completely to compost is expanded, and obtains bamboo grove organic fertilizer；
The production method of the improvement potato dextrose agar plate culture medium are as follows:
Boil bamboo grove dry branches and fallen leaves and each 20-30min of potato respectively with boiling water, and it is 150-200g/L that concentration is obtained by filtration respectively
Bamboo grove dry branches and fallen leaves water cooking liquid and 150-200g/L potato fruit；By the bamboo grove dry branches and fallen leaves water cooking liquid being obtained by filtration,
Water and potato fruit mixing, obtain mixed liquor；Agar is added into mixed liquor, and heats while stirring and melts completely to agar
Change, agar adds glucose after melting completely, and stirs evenly；Use concentration for the HCl of 0.1mol/L after mixing evenly and
It is 5-6 that the NaOH of 0.1mol/L, which adjusts pH value, and carries out sterilization treatment；It is cooling after sterilizing to obtain improvement potato dextrose agar
Plating medium.
2. the preparation method of bamboo grove organic fertilizer according to claim 1, which is characterized in that the bamboo grove dry branches and fallen leaves water
The volume ratio of boil liquid, water and potato fruit is 1-2:4-6:3-4.
3. the preparation method of bamboo grove organic fertilizer according to claim 1, which is characterized in that the potato, glucose
Mass ratio with agar is 15-20:1-2:1-2.
4. the preparation method of bamboo grove organic fertilizer according to claim 1, which is characterized in that will be pure described in step S05
Mycelium inoculation carries out culture into improvement potato dextrose agar fluid nutrient medium
Culture solution in every liter of improvement potato dextrose agar fluid nutrient medium is inoculated with the pure bacterium that 30-50 diameter is 6-9mm
Silk, and cultivated 12-15 days under conditions of temperature is 22-28 DEG C, revolving speed is 120-150r/min.
5. the preparation method of bamboo grove organic fertilizer according to claim 1, which is characterized in that will be upper described in step S06
It states liquid bacterial agent and is inoculated into solid culture medium to continue to cultivate and include:
Every kilogram of solid culture medium is inoculated with 400-600ml liquid bacterial agent, and continues culture solution under conditions of temperature is 22-28 DEG C
Body microbial inoculum.
6. the preparation method of bamboo grove organic fertilizer according to claim 5, which is characterized in that the solid culture medium includes
Mass ratio is the bamboo grove dry branches and fallen leaves and corn flour of 6-8:2-4.
7. the preparation method of bamboo grove organic fertilizer according to claim 1, which is characterized in that will be upper described in step S07
It states solid fungicide and is inoculated into expand in compost and include:
100-200g solid fungicide is inoculated in every kilogram of expansion compost.
8. the preparation method of bamboo grove organic fertilizer according to claim 7, which is characterized in that the expansion compost includes
Mass ratio is the bamboo grove dry branches and fallen leaves and trees dry branches and fallen leaves of 7-8:2-3, and the water content for expanding compost is 45-
60%.
9. the application method of bamboo grove organic fertilizer, which is characterized in that the bamboo grove as prepared by any one of claim 1-8 has
The application method of machine fertilizer includes:
It applies fertilizer in January December to next year；Scattered bamboos apply bamboo grove organic fertilizer 40-50kg using the method cultivated again is spread fertilizer over the fields per acre,
Chemical fertilizer 20-25kg；The method that Cluster Bamboo is applied using the outer cave of pocket, every clump of application bamboo grove organic fertilizer 1-2kg, chemical fertilizer 1-
2kg。
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