CN101948753A - Novel composite bacterial agent for degrading crop straw - Google Patents
Novel composite bacterial agent for degrading crop straw Download PDFInfo
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- CN101948753A CN101948753A CN2010102648909A CN201010264890A CN101948753A CN 101948753 A CN101948753 A CN 101948753A CN 2010102648909 A CN2010102648909 A CN 2010102648909A CN 201010264890 A CN201010264890 A CN 201010264890A CN 101948753 A CN101948753 A CN 101948753A
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- sacc
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Abstract
The invention discloses a fungal composite bacterial agent. The fungal composite bacterium agent is prepared by the following steps of: separating and purifying soil and a plant sample to obtain different fungal strains; selecting the fungal strains with high cellulose and ligninolytic enzyme generating capacity from the fungal strains; and optimally combining and proportioning to obtain cladosporium sphaerospermum fungus, auricularia polytricha and champgnon which can effectively degrade the lignin or the cellulose of the straw. The composite bacterial agent is applied to composting of the crop straw and can compost the crop straw in 10 to 15 days without the condition of high temperature.
Description
One, technical field
The invention belongs to technical field of microbe application, relate to complex microbial inoculum and produce the agricultural fertilizer technology, be used in particular for the rotten also fungi composite fungus agent of a kind of agricultural crop straw of degrading in field of stalk heap.
Two, technical background
Agricultural crop straw is a kind of renewable biological source and the non-competing resource of potential that is rich in effective constituent during farm crop produce.The photosynthetic product of farm crop has over half being present in the stalk, except most carbon, the organic matter that contains has Mierocrystalline cellulose, hemicellulose, xylogen, protein, fat and ash to grade, mineral element has nitrogen (0.5%-1.5%), phosphorus (0.1%-0.5%), potassium (0.74%-2.4%), magnesium (0.01%-0.4%), calcium (0.2%-0.5%), silicon (1%-5%) and sulphur (0.11%-0.35%) etc., is the renewable biological source that serves many purposes.Agricultural crop straw not only is rich in effective constituent, and output and total amount are very big, 1 kilogram of paddy rice or 1 kilogram of wheat can produce 1.5 kilograms of stalks, 1 kilogram of corn can produce 4 kilograms of maize straws, the annual all kinds of agricultural crop straw ultimate productions of China reach more than 700,000,000 tons, account for about 30% of the total stalk amount in the world, and wherein rice straw is about 2.3 hundred million tons, about 2.2 hundred million tons of maize straw, about 1.0 hundred million tons of stalks such as peanut and potato class tendril, beet leaves.Add up according to United Nations Environment Programme (UNEP) (UNEP), nearly 2,000,000,000 tons of the annual agricultural crop straw output in the whole world, major part all is not utilized effectively, in China, agricultural crop straw has less than 10% to be used as feed approximately, the stalk less than 3.0% that application technical processing utilizes, all the other most of stalks adopt burning disposal, and the whole nation is annual to have 2/3 stalk directly to be incinerated approximately.High temperature when stalk burns in the field can cause surface water to evaporate in a large number, destroy the drought-resistant moisture-preserving ability of soil, useful polypide in the soil and the survival of microorganism are impaired, thereby influence the benign cycle of soil plough horizon ecotope, moreover, the potential danger of bringing out fire of high temperature during burning, the smog of generation is all influential down to civil aviaton's landing to highway, railway, simultaneously the CO that produces behind the crop straw burning
2, CO, SO
2, NO
2, N
2Obnoxious flavoures such as O can cause serious air environmental pollution.Since the nineties in 20th century, along with increasing substantially of crop yield prediction, the stalk total amount is increasing sharply, and how this is abundant, reproducible Biological resources fully utilize agricultural crop straw, has been a subject matter in the farm crop production that faced jointly of the whole world.
In many research fields that agricultural straw resource utilizes, the rotten also technical study in field of stalk heap occupies important one seat.The rotten method of stalk heap mainly contains physics, chemistry and biological three major types, wherein best with applied microbiology method decomposition effect, just we pile rotten method by the normal stalk microbe of saying, this method is to utilize microbial decomposition, impel fermenting crops straws to become thoroughly decomposed, make elements such as organic matter contained in the stalk and nitrogen, phosphorus, potassium become the required nutrition of plant-growth, and breed a large amount of probiotics microorganisms, become good green and pile rotten fertilizer.Utilize microorganism to pile rotten method and make straw-returning, required nutrition of plant-growth and probiotics microorganism not only can be provided, and can improve guarantor's accumulation of energy power of soil moisture, improve soil property, increase soil aggregate, promote the increase of the soil organism and nitrogen, phosphorus, potassium equal size, improve tree characteristics.There is data to show, go back field maize straw 500kg for every mu, equal to use farmyard manure 2500kg, carbon ammonium 11.7kg, calcium superphosphate 6.2kg, vitriolate of tartar 4.75kg, soil organic matter content relatively increases by 0.05%～0.23% after 1 year, full phosphorus on average increases by 0.03%, available potassium increases 31.2mg/kg, the soil weight reduces by 0.03～0.16g/cm3, soil porosity increases 2%-4%.Continue straw-returning for many years, can improve phosphate fertilizer utilization efficiency, replenish the potash fertilizer deficiency, soil fertility can improve 0.5%～1% grade, and average amount of increase in production is more than 10%.Yet agricultural crop straw heap corruption is not for many years also used in the field fully, and wherein the reason of most critical is no matter be that any method all exists straw degradative to become thoroughly decomposed the time than long and very difficult problem completely.At present, the microbiobacterial agent used of many researchs of stalk microbe degraded and dropping into is mostly with the major objective that is degraded to of stalk cellulose.The research of decomposer and enzyme concentrates on the screening of cellulase strain, and as wooden enzyme, blue or green enzyme, inulinase and white-rot fungi cellulase etc., the microbial inoculum product is assert rotten bar spirit, CM bacterium, decomposition agent etc.But, Mierocrystalline cellulose, hemicellulose and xylogen all occupy larger proportion in the agricultural crop straw, its cellulose comprises 40%, hemicellulose and xylogen respectively account for about 20%, (Tengerdy R P, Szakacs G.Bioconversionof lignnocellulose in solid substrate fermentation[J] .Biochemical Engineering, 2003,13 (2-3): 167-179).And, cellulosic structure complexity in the stalk, it combines " lignocellulose " that formation is difficult to hydrolysis with xylogen, make the cellulase of microbiological degradation can't directly contact Mierocrystalline cellulose, fiber is usually mutually compound with the material of some more difficult decomposition in compost material, be difficult to by the most of microbe trans-utilization, add that hemicellulose links to each other with the tight of xylogen, the degradation effect of single microorganism is just more undesirable.(Hang Yiqiong etc. " white-rot fungi is to the degraded of rice straw and the variation of relevant enzymic activity "-" fungus system " 2001.20).The research of decomposing xylogen and cellulosic microorganism and enzyme at present focuses mostly on and is relying on traditional " enzyme is lived " screening, the enzyme of producing is widely used in washing, printing and dyeing and food-processing etc., fail to solve decomposing fast naturally of lignocellulose always, be used for rotten still less (the Zhang YHP of stalk heap, Himmel ME, MielenzJR.Outlook for cellu-lase improvement:Screening and selection strategies.Biotechnology Advances, 2006,24 (5): 452-481).People begin to note the research of flora and composite fungus agent in recent years, but much only be confined to laboratory test, and in the prior art, most of only the screening at a kind of in xylogen and the Mierocrystalline cellulose is used for degrading straw, cause in the straw degradative or cellulose degradation rate height and the lignin degradation rate is low, or lignin degradation rate height and cellulose degradation rate is low, can not satisfy the rotten actual demand of agricultural crop straw heap far away.Making full use of the conspiracy relation of the multiple microorganism of nature, filter out the compound system of efficient stable bacterial strain, with Mierocrystalline cellulose, hemicellulose and xylogen energy in the rotten process of the heap purpose synchronous, that degrade fast that reaches agricultural crop straw, is people's common objective.
Three, summary of the invention
The objective of the invention is to utilize the conspiracy relation between the microorganism, filter out and be applicable to rotten efficient degradation straw lignin and the cellulosic fungi composite fungus agent of agricultural crop straw heap.
The present invention adds soil and plant materials sample in the enrichment medium, obtain different fungal bacterial strains through separation and purification, therefrom filter out the fungal bacterial strain of high cellulase-producing or lignoenzyme, obtain the Auricularia polytricha (Mout) Sacc. Auricularia polytricha 916-1 and the mushroom Lentinula lateritia 939 of efficient degradation agricultural crop straw xylogen by the optimum combination proportioning, and the ball spore cladosporium Cladosporium sphaerospermum GC2-2 of efficient degradation crop branch fiber element, combine a kind of fungi composite fungus agent of novel degraded agricultural crop straw.Fungi composite fungus agent composition volume proportion is ball spore cladosporium 20%～25%, Auricularia polytricha (Mout) Sacc. 20%～25%, mushroom 50%～60%.
The present invention finishes by following technological line:
1, the collection of bacterial strain, separation and purification
Collected specimens on soil and the plant materials is preserved moisture and is transported the laboratory back, places sterilized water after 30 minutes in sample, adds fungi enrichment medium (peptone 10g, CMC-Na 10g, K
2HPO
41g, Na
2CO
35g, MgSO
47H
2O 0.1g, FeSO
47H
2O 0.015g, MnSO
40.05mg, yeast extract paste 10g, pH 6.0) in enrichment culture, obtain mixed bacteria liquid.
Adopt the method to 10 of gradient dilution step by step
-6, get 10
-3-10
-5On potato substratum (peeling potato 200g, sucrose 20g, agar 15-20g, pH nature), cultivate 4-5d under 30 ℃ of conditions, obtain different fungal colonies, further separation and purification obtains pure culture, obtains 15 fungus strains altogether.
2, the screening of bacterial strain
Adopt Congo red Mierocrystalline cellulose substratum (K
2HPO
40.50g, MgSO
40.25g, cellulose powder 1.88g, Congo red 0.20g, agar 14.00g, gelatin 2.00g, soil soaks juice 100ml, water 900ml, pH7.0) fungal bacterial strain of screening cellulase-producing, select in the size that Congo red Mierocrystalline cellulose substratum produces transparent circle according to bacterium colony, cellulase-producing high fungi transparent circle alive is big.Then the fungi of selecting is joined bacterial strain pure culture base (CMC-Na 10g, peptone 3.0g, KH
2PO
44.0g, MgSO
47H
2O 0.03g, water 1000ml, pH 6) middle shaking table cultivation, 28～30 ℃, 180r/min, time 3～4d obtains bacterium liquid.Draw 4ml bacterium liquid and add fermention medium (CMC-Na 10g, (NH
4)
28O
44g, KH
2PO
42g, MgSO
47H
2O0.5g, peptone 10g, extractum carnis 5g, water 1000ml) cultivate 48h, the method that every then 24h adopts filter paper enzyme activity and CMC enzyme to live is measured the plain enzyme of primary fiber and is lived, and surveys altogether 6 times.Repeatedly three times as stated above, finally obtain the fungi of 2 plant height cellulase-producings: a strain is that ball spore cladosporium Cladosporiumsphaerospermum GC2-2 (is called for short: ball spore cladosporium GC2-2, down together), an other strain is that aspergillus niger Aspergillus niger GC1-2 (is called for short: aspergillus niger GC1-2, down together), wherein the gene order of ball spore cladosporium GC2-2 is logined on the GenBank, and obtains accession number and be respectively HM347335.
Adopt PDA-Bavendamm color developing culture medium (peeling potato 200g, sucrose 20g, 0.4mmolL
-1Tannic acid, agar 15-20g, pH nature) screening can produce the fungal bacterial strain of lignoenzyme.The fungi that can produce lignoenzyme produces brown colour circle on the PDA-Bavendamm substratum, enzyme while still alive the big more generation of property to obtain the brown ring big more.Size screening according to the brown ring obtains the fungi that 4 plant heights produce lignoenzyme.The fungi that selects is joined bacterial strain pure culture base (Semen Maydis powder 2%, wheat bran 2%, glucose 1%, yeast extract paste 0.3%, potassium primary phosphate 0.1%, sal epsom 0.1%) shaking table is cultivated in, and 25-28 ℃, 180r/min, time 5～6d, obtain bacterium liquid, get 4-6ml bacterium liquid and join fermention medium (peeling horse bell 200g, KH
2PO
43g, MgSO
47H
2O3.1g, ammonium tartrate 5g, straw powder (crossing 60 orders) 20g, micro-50ml, pH5.0 (uses H
2SO
4Transfer)), cultivate 30d, adopt lignin peroxidase every three days, the method of manganese peroxidase and laccase activity is measured lignoenzyme and is lived, repeatedly three times as stated above, finally obtain 2 strains and produce the fungal bacterial strain of lignoenzyme, all belong to whiterot fungi, one strain is that Auricularia polytricha (Mout) Sacc. Auricularia polytricha916-1 (is called for short: Auricularia polytricha (Mout) Sacc. 916-1, down together), another strain is that mushroom Lentinula lateritia 939 (is called for short: mushroom 939, down together), wherein the gene order of Auricularia polytricha (Mout) Sacc. 916-1 and mushroom 939 has all been logined on the GenBank, and accession number is respectively HM347336 and HM347337.
3, fungi composite fungus agent proportioning
Ball spore cladosporium GC2-2 and aspergillus niger GC1-2 join bacterial strain pure culture base (CMC-Na10g, peptone 3.0g, KH respectively
2PO
44.0g, MgSO
47H
2O 0.03g, water 1000ml, pH 6) middle shaking table cultivation, 28 ℃～30 ℃, 180r/min, time 3～4d obtains ball spore cladosporium GC2-2 bacterium liquid and aspergillus niger GC1-2 bacterium liquid.
Auricularia polytricha (Mout) Sacc. 916-1 and mushroom 939 join bacterial strain pure culture base (Semen Maydis powder 2% respectively, wheat bran 2%, glucose 1%, yeast extract paste 0.3%, potassium primary phosphate 0.1%, sal epsom 0.1%) middle shaking table cultivation, 25-28 ℃, 180r/min, time 5～6d obtains Auricularia polytricha (Mout) Sacc. 916-1 bacterium liquid and mushroom 939 bacterium liquid.
Above-mentioned 4 kinds of bacterium liquid are carried out the proportioning combination cultivate, according to the various combination processing that every group of bacterium liquid thorough mixing is even, insert enzymatic production substratum (5% peptone, 0.5% Mierocrystalline cellulose, 0.5%NaCl, 0.2%CaCO respectively
3, 0.1% yeast powder, pH nature) in cultivate, measured an enzyme every 2 days and live, determine that best composite fungus agent forms.9 groups of combinations of 4 kinds of bacterium liquid are as table 1.
Table 1. proportioning combination table
By enzyme property mensuration while still alive, detect the 6th group of microbial inoculum and have antagonism, combination is produced the enzyme enzyme not as single bacterial strain alive and is lived high, the microbial inoculum of other 8 combinations all demonstrates synergetic property: the work of CMC enzyme enzyme is about 35 times that single bacterial strain enzyme is lived, filter paper enzyme activity is about 3 times that single bacterial strain enzyme is lived, and laccase activity and single bacterium colony are roughly the same.In these 8 combinations, record lignoenzyme during the single strain screening and have only a kind of laccase, after assembly, two new lignin-degrading enzymes-lignin peroxidases and manganese peroxidase have all been produced, infer that according to the bacterial strain that does not contain the white rot Pseudomonas in the 6th group of microbial inoculum appearance of these two kinds of lignin-degrading enzymes may specially to produce the characteristic of lignoenzyme relevant with whiterot fungi.
Select enzyme property is the highest while still alive the 7th group (ball spore cladosporium GC2-2, mushroom 939, auricularia auriculajudae 916-1) and combine the fungi composite fungus agent from these 8 combinations, the proportioning of three kinds of compositions is ball spore cladosporium GC2-220%～25%, Auricularia polytricha (Mout) Sacc. 916-120%～25%, mushroom 93950%～60%.After measured, the work of this fungi composite fungus agent CMC enzyme enzyme is up to 179U, and the work of filter paper enzyme enzyme is up to 121.4U, lignin peroxidase enzyme 44U alive, and manganese peroxidase enzyme 59.6U alive, laccase activity is 1.1U.
4. straw degradative test
The inoculum size of fungi composite fungus agent with 10%-20% joined in the straw powder, in about 30 ℃, observe and cultivated 30 days, measured an enzyme every 5 days and live, measure the degradation rate of Mierocrystalline cellulose and xylogen after 30 days.The mensuration of degradation rate adopts neutral detergent method, hydrochloric acid hydrolysis method, sulphuric acid hydrolysis method and ashing method, and measurement result is that cellulose degradation rate is 64.5%, and the degradation rate of xylogen is 27.8%.
5. the stalk stack retting test of becoming thoroughly decomposed
Stalk (corn, wheat stalk etc.) is broken into segment, press 1 ‰ of stalk weight～1.5 ‰ and add the fungi composite fungus agent, add 3 ‰～10 ‰ urea again to supply with nitrogenous source, water spray turns and keeps moisture 60%～75%, the thin plastic cloth of lid layer on the stalk heap covers the thin dust of giving birth to and macerates on the plastic cloth, turning keeps the humidity of compost for 3 times simultaneously during the stack retting, controlled trial does not connect the fungi composite fungus agent, and all the other operations are identical.Pile 30～35 ℃ of rotten temperature, the 10th, 15,20, the 30d sampling and measuring piles rotten index, each handles triplicate.Pile rotten index and weigh with the T value, the T value defined is the ratio of terminal point C/N ratio with initial C/N ratio, and the T value was less than 0.6 o'clock, and the heap corruption reaches the requirement of becoming thoroughly decomposed.By the T pH-value determination pH, use the fungi composite fungus agent to pile to reach in rotten agricultural crop straw 10-15 days and become thoroughly decomposed.
Fungi composite fungus agent of the present invention has synergistic effect, every cellulase activity and lignoenzyme are lived and all are better than single bacterial strain, optimized combination matching make its various enzymes while still alive property reach optimizing, and produced the enzyme-lignin peroxidase and the manganese peroxidase of two degradable straw lignins again, further increased degradation effect.
In the prior art, most of only the screening at a kind of in xylogen and the Mierocrystalline cellulose is used for degrading straw, cause in the straw degradative or cellulose degradation rate height and the lignin degradation rate is low, or lignin degradation rate height and cellulose degradation rate is low, the complexity of cellulosic structure in the stalk in addition, xylogen usually and hemicellulose lump together with chemical bonded refractory and be difficult for decomposition, so degradation effect is difficult to desirable, the bacterial strain of white rot Pseudomonas can produce efficient lignin-degrading enzymes in the fungi composite fungus agent of the present invention, promoted the degraded of straw lignin greatly, impel Mierocrystalline cellulose and xylogen to reach high degradation rate simultaneously, make the rotten effect of agricultural crop straw heap very good.
Bacterium source in the fungi composite fungus agent of the present invention is extensive, and preparation technology is simple, and preparation process is pollution-free and the three wastes produce, and application operating is convenient, and cost is low, and this fungi composite fungus agent uses wide, is easy to apply in agriculture production.The corruption of stalk heap does not need pyroprocessing when using, and just can reach the rotten effect of good heap about 30～35 ℃, piles the rotten time about 10-15 days, shortens than prior art and piles about the rotten 5 days time.
Four, embodiment
Embodiment 1
1. the preparation of composite fungus agent
Ball spore cladosporium GC2-2 joins bacterial strain pure culture base (CMC-Na 10g, peptone 3.0g, KH
2PO
44.0g, MgSO
47H
2O 0.03g, water 1000ml, pH 6) middle shaking table cultivation, 30 ℃, 180r/min, time 4d obtains ball spore cladosporium GC2-2 bacterium liquid.
Auricularia polytricha (Mout) Sacc. 916-1 and mushroom 939 join bacterial strain pure culture base (Semen Maydis powder 2%, wheat bran 2%, glucose 1%, yeast extract paste 0.3% respectively, potassium primary phosphate 0.1%, sal epsom 0.1%) middle shaking table cultivation, 26 ℃, 180r/min, time 6d obtains Auricularia polytricha (Mout) Sacc. 916-1 bacterium liquid and mushroom 939 bacterium liquid.
Above three kinds of bacterium liquid are even according to 22% ball spore cladosporium GC2-2,23% Auricularia polytricha (Mout) Sacc. 916-1,55% mushroom, 939 ratio thorough mixing, obtain composite fungus agent.
2. the heap corruption of stalk
The 500kg maize straw is ground into segment, add the fungi composite fungus agent by 1.2 ‰, add 4 ‰ urea again, add water management humidity about 65%, stalk stacks, and covers one deck plastic film then, one deck thin dust of giving birth in plastic film upper berth is macerated, turning keeps the humidity of compost for 3 times simultaneously during the stack retting, and 13 days left and right sides T values are less than 0.6, and stalk reaches and becomes thoroughly decomposed.
Embodiment 2
1. the preparation of composite fungus agent
Ball spore cladosporium GC2-2 joins bacterial strain pure culture base (CMC-Na 10g, peptone 3.0g, KH
2PO
44.0g, MgSO
47H
2O 0.03g, water 1000ml, pH 6) middle shaking table cultivation, 30 ℃, 180r/min, time 4d obtains ball spore cladosporium GC2-2 bacterium liquid.
Auricularia polytricha (Mout) Sacc. 916-1 and mushroom 939 join bacterial strain pure culture base (Semen Maydis powder 2%, wheat bran 2%, glucose 1%, yeast extract paste 0.3% respectively, potassium primary phosphate 0.1%, sal epsom 0.1%) middle shaking table cultivation, 26 ℃, 180r/min, time 6d obtains Auricularia polytricha (Mout) Sacc. 916-1 bacterium liquid and mushroom 939 bacterium liquid.
Above three kinds of bacterium liquid are even according to 25% ball spore cladosporium GC2-2,25% Auricularia polytricha (Mout) Sacc. 916-1,50% mushroom, 939 ratio thorough mixing, obtain composite fungus agent.
2. the heap corruption of stalk
The 500kg maize straw is ground into segment, add the fungi composite fungus agent by 1.4 ‰, add 4 ‰ urea again, add water management humidity about 65%, stalk stacks, and covers one deck plastic film then, one deck thin dust of giving birth in plastic film upper berth is macerated, turning keeps the humidity of compost for 3 times simultaneously during the stack retting, and 11 days left and right sides T values are less than 0.6, and stalk reaches and becomes thoroughly decomposed.
Claims (3)
1. the composite fungus agent of a novel degraded agricultural crop straw, it is characterized in that, described composite fungus agent is obtained by ball spore cladosporium, Auricularia polytricha (Mout) Sacc., Xianggu mushroom strain proportioning, wherein ball spore cladosporium: Auricularia polytricha (Mout) Sacc.: the volume ratio of mushroom is 20%～25%: 20%～25%: 50%～60%.
2. the composite fungus agent of the novel degraded agricultural crop straw of claim 1, it is characterized in that, described Auricularia polytricha (Mout) Sacc., mushroom, ball spore cladosporium are respectively Auricularia polytricha (Mout) Sacc. Auricularia polytricha 916-1, mushroom Lentinula lateritia 939, ball spore cladosporium Cladosporium sphaerospermum GC2-2.
3. the composite fungus agent of the described novel degraded agricultural crop straw of claim 2, it is characterized in that, described Auricularia polytricha (Mout) Sacc., mushroom, ball spore cladosporium are that soil and plant materials sample are added the fungi enrichment medium, through the fungal bacterial strain that separation and purification screening obtains, the consisting of of fungi enrichment medium: peptone 10g, CMC-Na 10g, K
2HPO
41g, Na
2CO
35g, MgSO
47H
2O0.1g, FeSO
47H
2O 0.015g, MnSO
40.05mg, yeast extract paste 10g, the pH value is 6.
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| CN102911877A (en) * | 2012-08-27 | 2013-02-06 | 浙江工业大学 | Marine fungi cladosporium sphaerospermum and application thereof |
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| CN103540573B (en) * | 2013-10-19 | 2015-10-07 | 沅江浣溪沙酶技术有限公司 | A kind of lignoenzyme and production method |
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| CN103951477B (en) * | 2014-03-28 | 2016-07-20 | 甘肃富民生态农业科技有限公司 | A kind of straw decomposing inoculant and production method thereof |
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| CN105198508B (en) * | 2015-10-29 | 2019-01-22 | 湖南省森林植物园 | A kind of preparation method and application method of bamboo grove organic fertilizer |
| CN107500811A (en) * | 2017-08-30 | 2017-12-22 | 河南师范大学 | A kind of preparation method of glutinous rehmannia root swelling tailored version microbial-bacterial fertilizer |
| CN109805158A (en) * | 2019-01-16 | 2019-05-28 | 中国农业科学院北京畜牧兽医研究所 | A kind of method and application using fungal biodegradation corn stalk fiber |
| CN109626555A (en) * | 2019-01-21 | 2019-04-16 | 四川清和科技有限公司 | A kind of method of fast degradation water body withered fallen leaf |
| CN109837218A (en) * | 2019-03-29 | 2019-06-04 | 南京晓庄学院 | A kind of mould SP02001 of rice straw efficient degrading bacteria branch spore and its application |
| CN109837218B (en) * | 2019-03-29 | 2023-06-27 | 南京晓庄学院 | Rice straw efficient degradation fungus cladosporium SP02001 and application thereof |
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