CN105166568A - Micro-ecological feed for preventing bacillary enteritis of grass carps - Google Patents
Micro-ecological feed for preventing bacillary enteritis of grass carps Download PDFInfo
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- CN105166568A CN105166568A CN201510657998.7A CN201510657998A CN105166568A CN 105166568 A CN105166568 A CN 105166568A CN 201510657998 A CN201510657998 A CN 201510657998A CN 105166568 A CN105166568 A CN 105166568A
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Abstract
The invention relates to micro-ecological feed for preventing bacillary enteritis of grass carps. A feed formulation can completely meet the nutritional requirements of the grass carps. Added active compound lactobacillus powder can effectively improve the intestinal structure and the digestive performance of the intestinal tract and maintain the physiological structure stability of intestinal villus. It is proved through the test that when the grass carps are fed with the micro-ecological feed, the thickness of the intestinal mucosa of the grass carps can be remarkably increased, and the morbidity of bacillary enteritis caused by the fact that the intestinal tissue serves as an infection invasion channel can be effectively controlled.
Description
[technical field]
The present invention relates to a kind of feed of grass carp, particularly relate to a kind of micro-ecological feed preventing grass carp bacillary enteritis, belong to field of animal feed.
[background technology]
Grass carp commercial feed can meet the normal growth physiological requirements of grass carp, and adequate nutrition arranging scheme can effectively reduce grass carp feed coefficient, reduces feed cost.But in disease popularity season, the grass carp bacillary enteritis that frequency of disease development tops the list only has just can realize effective prevention and control by admixing curative drug in feed.
Grass carp bacillary enteritis refers to the class disease being caused enteritis by common bacterial pathogen infection, be mainly in Chun Mo and more period in early summer rainy season, and there is infectiousness, the death rate is generally 5-25%; characteristics of lesion main manifestations is that enteron aisle is congested, hemorrhage, the necrosis of intestinal villus epithelial cell and interstitial inflammation cellular infiltration.The generation of this disease many with breeding environment, the factor such as the virulence of the healthy and bacterial pathogen of grass carp intestinal is closely related.Therefore, when breeding environment deterioration, bacterial pathogen reproductive capacity and virulence strengthen, strengthen grass carp physical health for the probability of happening effectively preventing bacillary enteritis, especially continuative improvement or enhancing grass carp intestinal health, the physical barriers function promoting the disease-resistant former invasion of enteron aisle is significant.
[summary of the invention]
For above problem, the object of the present invention is to provide a kind of micro-ecological feed preventing grass carp bacillary enteritis.
For achieving the above object, the present invention is by the following technical solutions:
A kind of micro-ecological feed preventing grass carp bacillary enteritis; example meter in mass ratio, comprises following component: fish meal 1-8 part, chicken meal 6-12 part, dregs of beans 10-20 part, dish dregs of rice 18-30 part, DDGS5-15 part, wheat 8-18 part, system chaff 4-8 part, soya-bean oil 1-2 part, calcium dihydrogen phosphate 1-3 part, Choline Chloride 0.1-0.2 part, multidimensional premix 0.2 part, many ore deposits premix 0.2 part, taurine 0.01 part, phytase 0.02-0.1 part, compound lactobacillus powder 0.1-0.2 part, flour 4-8 part.
Preferably, described active compound lactobacillus powder, comprises streptococcus lactis, Bacillus acidi lactici, food grade organic acid and maize cob meal.
Preferably, in described active compound lactobacillus powder, nisin bacterial content is 10
12more than cfu/g, Bacillus acidi lactici content is 10
12more than cfu/g, food grade organic acid's content is 15%, maize cob meal 45%-65%.
Described system chaff is commercially available common system chaff, and wherein the ratio of rice bran and rice chaff powder is 2:8 (sixteen unite chaff) or 3:7 (pseudo-ginseng unite chaff).
As a kind of preferred version; the micro-ecological feed of described prevention grass carp bacillary enteritis, is made up of the component of following weight portion: fish meal 6 parts, chicken meal 9 parts, dregs of beans 15 parts, the dish dregs of rice 24 parts, DDGS15 part, wheat 16 parts, system chaff 6 parts, 2 parts, soya-bean oil, calcium dihydrogen phosphate 1 part, 88 Choline Chloride 0.1 part, multidimensional premix 0.2 part, many ore deposits premix 0.2 part, taurine 0.01 part, phytase 0.02 part, 0.17 part, compound lactobacillus powder, 5.3 parts, flour.
Described multidimensional premix and the premix of many ore deposits are common commercially available general vitamin premix and mineral substance premix.
Wherein, a kind of preferred vitamin premix is that every kilogram of vitamin premix comprises: 3-Hydroxyretinol 0g, vitamine D1 5g, vitamin e1 00g, vitamin K1 5g, nicotinic acid 100g, calcium pantothenate 50g, vitamin B12 0g, vitamin B2 25g, vitamin B6 20g, vitamin B12 0.2g, biotin 0.4g, folic acid 5g, vitamin C 100g, inositol 100g, defatted rice bran 419.4g.
A kind of preferred mineral substance premix is that every kilogram of mineral substance premix comprises: five water magnesium sulfate 50g, ferrous sulfate monohydrate 30g, monohydrate zinc sulphate 30g, manganese sulfate monohydrate 10g, sodium selenite 6.7g, CoCL2 6H2O 2.5g, KI 8g, defatted rice bran 862.8g.
Beneficial effect:
Compared with prior art, the present invention has following beneficial effect:
1, feed formula of the present invention meets the nutritional need of grass carp completely, and the active compound lactobacillus powder of interpolation effectively can improve intestinal tissue, promotes enteron aisle digestic property, and the physiological structure maintaining intestinal villus is stablized.Test proves, micro-ecological feed of the present invention of throwing something and feeding, can significantly improve grass carp intestinal mucosa thickness, is that the incidence of disease infecting the bacillary enteritis that invasive channel causes can be controlled effectively with intestinal tissue.
2, the present invention adds active compound lactobacillus powder, contained member lactic acid bacteria is the main probio of fish body alimentary canal and genital tract mucous membrane, there is effects such as maintaining microecological balance, immunity moderation and short digestion, the field planting of exogenous pathogen, displacement and infection can be suppressed, with other raw material reasonable compatibilities, intestinal mucosa thickness can be significantly improved, to the integrality of fish body intestinal mucosa, there is good maintenance effects, effectively prevent the bacillary enteritis that invasive cause of disease causes.
In addition, feed environmentally safe of the present invention, belongs to environmental type product, and preparation method is conventional method, easy to prepare.
Accompanying drawing explanation
Fig. 1 is the intestinal morphology of grass carp when on-test in culture experiment of the present invention in No. 1 pond;
Fig. 2 is the intestinal morphology of grass carp when on-test in culture experiment of the present invention in No. 5 ponds;
Fig. 3 is the intestinal morphology of grass carp when on-test in culture experiment of the present invention in No. 6 ponds;
Fig. 4 is the intestinal morphology of grass carp when off-test in culture experiment of the present invention in No. 1 pond;
Fig. 5 is the intestinal morphology of grass carp when off-test in culture experiment of the present invention in No. 5 ponds;
Fig. 6 is the intestinal morphology of grass carp when off-test in culture experiment of the present invention in No. 6 ponds.
[detailed description of the invention]
Below in conjunction with specific embodiment, the present invention is further described.Unless stated otherwise, the present invention adopts reagent, equipment and method are the conventional commercial reagent of the art, equipment and the conventional method used.
Embodiment 1
By weight, fish meal 1 part, chicken meal 12 parts, dregs of beans 10 parts, the dish dregs of rice 30 parts, DDGS9.09 part, wheat 18 parts, system chaff 8 parts, 1 part, soya-bean oil, calcium dihydrogen phosphate 2 parts, Choline Chloride 0.2 part, multidimensional premix 0.2 part, many ore deposits premix 0.2 part, taurine 0.01 part, phytase 0.1 part, 0.2 part, compound lactobacillus powder, 8 parts, flour is taken respectively.Described system chaff is sixteen system chaffs, and in described compound lactobacillus powder, nisin bacterial content is 10
12cfu/g, Bacillus acidi lactici content is 10
12cfu/g, food grade organic acid's content is 15%, maize cob meal 65%, mixes in proportion after being pulverized by above-mentioned raw materials, by conventional feed machinery, namely can be made into micro-ecological feed of the present invention after extruding cutting.
Wherein, every kilogram of above-mentioned multidimensional premix comprises: 3-Hydroxyretinol 0g, vitamine D1 5g, vitamin e1 00g, vitamin K1 5g, nicotinic acid 100g, calcium pantothenate 50g, vitamin B12 0g, vitamin B2 25g, vitamin B6 20g, vitamin B12 0.2g, biotin 0.4g, folic acid 5g, vitamin C 100g, inositol 100g, defatted rice bran 419.4g.Every kilogram of above-mentioned many ore deposits premix comprises: five water magnesium sulfate 50g, ferrous sulfate monohydrate 30g, monohydrate zinc sulphate 30g, manganese sulfate monohydrate 10g, sodium selenite 6.7g, CoCL2 6H2O 2.5g, KI 8g, defatted rice bran 862.8g.
Embodiment 2
By weight, fish meal 6 parts, chicken meal 9 parts, dregs of beans 15 parts, the dish dregs of rice 24 parts, DDGS15 part, wheat 16 parts, system chaff 6 parts, 2 parts, soya-bean oil, calcium dihydrogen phosphate 1 part, Choline Chloride 0.1 part, multidimensional premix 0.2 part, many ore deposits premix 0.2 part, taurine 0.01 part, phytase 0.02 part, 0.17 part, compound lactobacillus powder, 5.3 parts, flour is taken respectively.Described system chaff is pseudo-ginseng system chaff, and in described compound lactobacillus powder, nisin bacterial content is 10
13cfu/g, Bacillus acidi lactici content is 10
13cfu/g, food grade organic acid's content is 15%, maize cob meal 55%, mixes in proportion after being pulverized by above-mentioned raw materials, by conventional feed machinery, namely can be made into micro-ecological feed of the present invention after extruding cutting.
Wherein, every kilogram of above-mentioned multidimensional premix comprises: 3-Hydroxyretinol 0g, vitamine D1 5g, vitamin e1 00g, vitamin K1 5g, nicotinic acid 100g, calcium pantothenate 50g, vitamin B12 0g, vitamin B2 25g, vitamin B6 20g, vitamin B12 0.2g, biotin 0.4g, folic acid 5g, vitamin C 100g, inositol 100g, defatted rice bran 419.4g.Every kilogram of above-mentioned many ore deposits premix comprises: five water magnesium sulfate 50g, ferrous sulfate monohydrate 30g, monohydrate zinc sulphate 30g, manganese sulfate monohydrate 10g, sodium selenite 6.7g, CoCL2 6H2O 2.5g, KI 8g, defatted rice bran 862.8g.
Embodiment 3
By weight, fish meal 8 parts, chicken meal 6 parts, dregs of beans 20 parts, the dish dregs of rice 29.8 parts, DDGS5 part, wheat 18 parts, system chaff 4 parts, 1.5 parts, soya-bean oil, calcium dihydrogen phosphate 3 parts, Choline Chloride 0.15 part, multidimensional premix 0.2 part, many ore deposits premix 0.2 part, taurine 0.01 part, phytase 0.04 part, 0.1 part, compound lactobacillus powder, 4 parts, flour is taken respectively.Described system chaff is sixteen system chaffs, and in described compound lactobacillus powder, nisin bacterial content is 5 × 10
12cfu/g, Bacillus acidi lactici content is 10
13cfu/g, food grade organic acid's content is 15%, maize cob meal 45%, mixes in proportion after being pulverized by above-mentioned raw materials, by conventional feed machinery, namely can be made into micro-ecological feed of the present invention after extruding cutting.
Embodiment 4
By weight, fish meal 5.18 parts, chicken meal 12 parts, dregs of beans 20 parts, the dish dregs of rice 18 parts, DDGS15 part, wheat 8 parts, system chaff 8 parts, 2 parts, soya-bean oil, calcium dihydrogen phosphate 3 parts, Choline Chloride 0.2 part, multidimensional premix 0.2 part, many ore deposits premix 0.2 part, taurine 0.01 part, phytase 0.08 part, 0.13 part, compound lactobacillus powder, 8 parts, flour is taken respectively.Described system chaff is pseudo-ginseng system chaff, and in described compound lactobacillus powder, nisin bacterial content is 10
13cfu/g, Bacillus acidi lactici content is 6 × 10
13cfu/g, food grade organic acid's content is 15%, maize cob meal 50%, mixes in proportion after being pulverized by above-mentioned raw materials, by conventional feed machinery, namely can be made into micro-ecological feed of the present invention after extruding cutting.
Culture experiment
1, test method:
In cultivation base, sea-gull island one, Fanyu, selection water surface for aquaculture is six pieces of ponds of about 10 mu.These six pieces of pond culture density are identical, and every mu of grass carp, variegated carp and silver carp are respectively 1200 tails, 50 tails and 100 tails, and wherein the initial stocking size of grass carp is the equal 50-80g of tail.Above-mentioned six pieces of ponds respectively random number are 1,2,3,4,5, No. 6 pond, wherein, 1, throw something and feed the micro-ecological feed of prevention grass carp bacillary enteritis prepared by embodiment 1,2,3,4 respectively in 2,3, No. 4 ponds, common grass carp commercial feed A, the B identical with the present invention from the protein tag content of Liang Ge feed producer of throwing something and feeding commercially respectively in 5, No. 6 ponds.60 days test periods, test the April-May (grass carp bacillary enteritis multiple phase) in period.
At the end of experimental period, from six ponds, fish for 10 tail grass carps respectively at random dissect, before, during and after getting, intestines enteron aisle is cut into slices, and measures the grass carp intestinal tissue mucosal thickness in different pond.Duration of test, records the forage volume of throwing something and feeding of every day, grass carp morbidity quantity and dead quantity.On-test and at the end of, from 1,5, No. 6 pond, fish for 10 tail grass carps at random respectively, solution takes anterior intestine and does histotomy, knits form for what respectively organize grass carp group enteron aisle.
2, result of the test:
Each pond grass carp intestinal mucosa thickness is as shown in table 1, compared with the pond grass carp of throw something and feed commercial feed A, B, the grass carp anterior intestine of micro-ecological feed prepared by embodiment of throwing something and feeding 1,2,3,4, middle intestines and hindgut intestinal mucosa thickness all significantly raise, wherein, micro-ecological feed prepared by embodiment 2 improves the most remarkable, this explanation, use micro-ecological feed of the present invention, grass carp intestinal mucosa thickness can be significantly improved, add the surface area of intestinal villus, thus effectively promote digestion surface area and the digestibility of intestinal tissue.
Table 1 each pond grass carp intestinal mucosa thickness
| Group | To throw something and feed feed | Anterior intestine (μm) | Middle intestines (μm) | Hindgut (μm) |
| No. 1 pond | Feed prepared by embodiment 1 | 622.38 | 531.67 | 380.26 |
| No. 2 ponds | Feed prepared by embodiment 2 | 644.23 | 544.52 | 385.12 |
| No. 3 ponds | Feed prepared by embodiment 3 | 632.50 | 531.39 | 379.71 |
| No. 4 ponds | Feed prepared by embodiment 4 | 628.92 | 541.57 | 372.55 |
| No. 5 ponds | Commercial feed A | 495.83 | 493.22 | 311.09 |
| No. 6 ponds | Commercial feed B | 551.45 | 486.33 | 313.36 |
The duration of test each pond death condition of morbidity grass carp through being accredited as bacillary enteritis is as shown in table 2, it can thus be appreciated that, compared with commodity in use feed A, B, using micro-ecological feed of the present invention effectively can reduce the death rate of the onset of bacillary enteritis, is that the bacillary enteritis incidence of disease infecting invasive channel initiation can be controlled effectively with intestinal tissue.
Table 2 duration of test each pond morbidity grass carp is through being accredited as the death condition of bacillary enteritis
| Group | To throw something and feed feed | Morbidity mortality (tail) per mu |
| No. 1 pond | Feed prepared by embodiment 1 | 2 |
| No. 2 ponds | Feed prepared by embodiment 2 | 1 |
| No. 3 ponds | Feed prepared by embodiment 3 | 2 |
| No. 4 ponds | Feed prepared by embodiment 4 | 3 |
| No. 5 ponds | Commercial feed A | 11 |
| No. 6 ponds | Commercial feed B | 8 |
As Figure 1-3, during on-test, the grass carp intestinal tissue in 1,5, No. 6 pond all belongs to normal histology category, and intestinal mucosa, intestinal villus, epithelial cell, goblet cell, submucosa, muscle layer, placenta percreta structural intergrity are good.As Figure 4-Figure 6, when testing the 60th day, No. 5 pond grass carp intestinal mucosa generation focal lesions, main manifestations is that focal intestinal villus end epithelial cell is downright bad, intestinal villus fracture, but intestinal tissue does not observe the phenomenons such as hemorrhage or inflammation; The focal fracture of No. 6 pond grass carp intestinal villus, come off, intestinal villus end epithelial cell is downright bad, and intestinal villus both sides tissue is that obvious zigzag invertibity pathologic changes; And the grass carp intestinal mucosa in No. 1 pond, intestinal villus, epithelial cell, goblet cell, submucosa, muscle layer, placenta percreta structural intergrity are good.This illustrates, micro-ecological feed prepared by embodiment of throwing something and feeding 1, is conducive to grass carp intestinal tissue and maintains normal structure form, promote the lifting of grass carp intestinal digestion performance.
The above; be only the preferably specific embodiment of invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and design thereof and replace or change, all should be encompassed in protection scope of the present invention.
Claims (6)
1. one kind is prevented the micro-ecological feed of grass carp bacillary enteritis; it is characterized in that; example meter in mass ratio, comprises following component: fish meal 1-8 part, chicken meal 6-12 part, dregs of beans 10-20 part, dish dregs of rice 18-30 part, DDGS5-15 part, wheat 8-18 part, system chaff 4-8 part, soya-bean oil 1-2 part, calcium dihydrogen phosphate 1-3 part, Choline Chloride 0.1-0.2 part, multidimensional premix 0.2 part, many ore deposits premix 0.2 part, taurine 0.01 part, phytase 0.02-0.1 part, compound lactobacillus powder 0.1-0.2 part, flour 4-8 part.
2. the micro-ecological feed of prevention grass carp bacillary enteritis according to claim 1, it is characterized in that, described active compound lactobacillus powder, comprises streptococcus lactis, Bacillus acidi lactici, food grade organic acid and maize cob meal.
3. the micro-ecological feed of prevention grass carp bacillary enteritis according to claim 2, it is characterized in that, in described active compound lactobacillus powder, nisin bacterial content is 10
12more than cfu/g, Bacillus acidi lactici content is 10
12more than cfu/g, food grade organic acid's content is 15%, maize cob meal 45%-65%.
4. the micro-ecological feed of prevention grass carp bacillary enteritis according to claim 3; it is characterized in that, the micro-ecological feed of described prevention grass carp bacillary enteritis is made up of the component of following weight portion: fish meal 6 parts, chicken meal 9 parts, dregs of beans 15 parts, the dish dregs of rice 24 parts, DDGS15 part, wheat 16 parts, system chaff 6 parts, 2 parts, soya-bean oil, calcium dihydrogen phosphate 1 part, 88 Choline Chloride 0.1 part, multidimensional premix 0.2 part, many ore deposits premix 0.2 part, taurine 0.01 part, phytase 0.02 part, 0.17 part, compound lactobacillus powder, 5.3 parts, flour.
5. the micro-ecological feed of the prevention grass carp bacillary enteritis according to any one of claim 1-4, it is characterized in that, comprise in multidimensional premix described in every kilogram: 3-Hydroxyretinol 0g, vitamine D1 5g, vitamin e1 00g, vitamin K1 5g, nicotinic acid 100g, calcium pantothenate 50g, vitamin B12 0g, vitamin B2 25g, vitamin B6 20g, vitamin B12 0.2g, biotin 0.4g, folic acid 5g, vitamin C 100g, inositol 100g, defatted rice bran 419.4g.
6. the micro-ecological feed of the prevention grass carp bacillary enteritis according to any one of claim 1-4, it is characterized in that, many ore deposits premix described in every kilogram comprises: five water magnesium sulfate 50g, ferrous sulfate monohydrate 30g, monohydrate zinc sulphate 30g, manganese sulfate monohydrate 10g, sodium selenite 6.7g, CoCL2 6H2O 2.5g, KI 8g, defatted rice bran 862.8g.
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| CN106954761A (en) * | 2017-03-30 | 2017-07-18 | 广州市海维饲料有限公司 | A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof |
| CN107114617A (en) * | 2017-06-25 | 2017-09-01 | 广州市诚水产科技有限公司 | A kind of grass carp compound premixed feed and its application |
| CN107712472A (en) * | 2017-11-27 | 2018-02-23 | 山东海博农牧科技有限公司 | It is a kind of to prevent and treat easy absorption feed of grass carp combined and preparation method thereof |
| CN110506837A (en) * | 2019-09-30 | 2019-11-29 | 江门海大饲料有限公司 | It is a kind of to prevent functional feed additive that grass carp irritability tail fin whitens and functional feed and application |
| CN111758857A (en) * | 2020-07-29 | 2020-10-13 | 四川农业大学 | Dietary composition for preventing and treating bacterial enteritis of grass carps and application thereof |
| CN112335800A (en) * | 2020-12-08 | 2021-02-09 | 通威股份有限公司 | Compound feed for promoting proliferation of intestinal lactic acid bacteria of grass carp and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106954761A (en) * | 2017-03-30 | 2017-07-18 | 广州市海维饲料有限公司 | A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof |
| CN107114617A (en) * | 2017-06-25 | 2017-09-01 | 广州市诚水产科技有限公司 | A kind of grass carp compound premixed feed and its application |
| CN107712472A (en) * | 2017-11-27 | 2018-02-23 | 山东海博农牧科技有限公司 | It is a kind of to prevent and treat easy absorption feed of grass carp combined and preparation method thereof |
| CN110506837A (en) * | 2019-09-30 | 2019-11-29 | 江门海大饲料有限公司 | It is a kind of to prevent functional feed additive that grass carp irritability tail fin whitens and functional feed and application |
| CN111758857A (en) * | 2020-07-29 | 2020-10-13 | 四川农业大学 | Dietary composition for preventing and treating bacterial enteritis of grass carps and application thereof |
| CN112335800A (en) * | 2020-12-08 | 2021-02-09 | 通威股份有限公司 | Compound feed for promoting proliferation of intestinal lactic acid bacteria of grass carp and preparation method thereof |
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Application publication date: 20151223 |
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