CN106954761A - A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof - Google Patents
A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof Download PDFInfo
- Publication number
- CN106954761A CN106954761A CN201710200953.6A CN201710200953A CN106954761A CN 106954761 A CN106954761 A CN 106954761A CN 201710200953 A CN201710200953 A CN 201710200953A CN 106954761 A CN106954761 A CN 106954761A
- Authority
- CN
- China
- Prior art keywords
- parts
- grass carp
- intestinal villus
- extract
- feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of ecological feed for repairing grass carp intestinal villus, include the component of following parts by weight：10 17 parts of chicken meal, 13 parts of squid liver paste, 12 22 parts of fermented bean dregs, 20 35 parts of rapeseed meal, 5 12 parts of expanded cotton dregs, 5 15 parts of DGGS, 8 18 parts of wheat, 14 parts of soy sauce residues, 12 parts of soya-bean oil, 13 parts of calcium dihydrogen phosphate, 0.1 0.2 parts of Choline Chloride, 0.15 0.2 parts of multidimensional premix, 0.15 0.2 parts of many ore deposit premixes, 0.15 0.2 parts of endotoxin adsorbent, 48 parts of flour, 0.1 0.5 parts of lactobacillus-fermented product complex, 13 parts of intestinal villus tissue repair activator.It is a kind of as present invention offer effectively to repair the ecological feed of grass carp intestinal villus, by repairing grass carp intestinal villus, strengthen enteron aisle digestive function, effectively improve the feed conversion rate of grass carp, reduce feed coefficient, reduce the enteritis incidence of disease.
Description
Technical field
The present invention relates to a kind of grass carp ecological feed, in particular it relates to a kind of ecological feed for repairing grass carp intestinal villus and
Its preparation method.
Background technology
Grass carp is first of the large Chinese carp of China four, to propagate region artificially and be distributed mainly on south China, Central China, East China and southwest ground
Area, is one of important large freshwater aquiculture kind of China.According to 2014 China Fisheries statistical yearbooks, grass carp cultured output is about 507
Ten thousand tons, first is sure to occupy in Fish-water Fish Farming yield.
With the development and upgrading of perfect compound feed technology, whole feed has been fully achieved in the grass carp culture-cycle
Feed, and within the different breeding stage, the feed product of Different Nutrition class enriches very much.However, different feed products
Or but there are different influences to grass carp health status in the feed product of Different Nutrition class.
Grass carp alimentary canal is the important place that feed is stored, digests, absorbs and drain, and its health degree directly affects feed
Conversion ratio and fish body physical health etc..
The content of the invention
In a first aspect, the present invention provides a kind of ecological feed for repairing grass carp intestinal villus.
A kind of ecological feed for repairing grass carp intestinal villus, includes the component of following parts by weight：10-17 parts of chicken meal, squid
1-3 parts of cream, 12-22 parts of fermented bean dregs, 20-35 parts of rapeseed meal, expanded cotton dregs 5-12 parts, distiller's dried grain and its DDGS (DDGS) 5-15
Part, 8-18 parts of wheat, 1-4 parts of soy sauce residues, 1-2 parts of soya-bean oil, 1-3 parts of calcium dihydrogen phosphate, 0.1-0.2 parts of Choline Chloride, multidimensional are pre-
0.15-0.2 parts of batch mixing, 0.15-0.2 parts of many ore deposit premixes, 0.15-0.2 parts of endotoxin adsorbent, 4-8 parts of flour, lactobacillus-fermented
0.1-0.5 parts of product complex, 1-3 parts of intestinal villus tissue repair activator.
It is preferred that, a kind of ecological feed for repairing grass carp intestinal villus includes the component of following parts by weight：15 parts of chicken meal,
2 parts of squid liver paste, 20 parts of fermented bean dregs, 30 parts of rapeseed meal, 10 parts of expanded cotton dregs, distiller's dried grain and its 5 parts of DDGS (DDGS), wheat 8
Part, 1 part of soy sauce residues, 1 part of soya-bean oil, 1.7 parts of calcium dihydrogen phosphate, 0.2 part of Choline Chloride, 0.2 part of multidimensional premix, many ore deposit premixes
0.2 part, 0.2 part of endotoxin adsorbent, 4 parts of flour, 0.5 part of lactobacillus-fermented product complex, intestinal villus tissue repair activator 1
Part.
It is preferred that, a kind of ecological feed for repairing grass carp intestinal villus includes the component of following parts by weight：13 parts of chicken meal,
3 parts of squid liver paste, 18 parts of fermented bean dregs, 35 parts of rapeseed meal, 7 parts of expanded cotton dregs, distiller's dried grain and its 4 parts of DDGS (DDGS), wheat 8
Part, 1 part of soy sauce residues, 1 part of soya-bean oil, 1.7 parts of calcium dihydrogen phosphate, 0.2 part of Choline Chloride, 0.2 part of multidimensional premix, many ore deposit premixes
0.2 part, 0.2 part of endotoxin adsorbent, 4 parts of flour, 0.3 part of lactobacillus-fermented product complex, intestinal villus tissue repair activator
1.2 parts.
It is preferred that, above-mentioned lactobacillus-fermented product complex includes following parts by weight of component：Streptococcus cremoris tunning
40-45 parts, 30-35 parts of lactic acid leukonid tunning, 20-25 parts of bifidobacterium fermentation product.
It is further preferred that above-mentioned lactobacillus-fermented product complex includes following parts by weight of component：Streptococcus cremoris is sent out
45 parts of ferment product, 35 parts of lactic acid leukonid tunning, 20 parts of bifidobacterium fermentation product.
It is further preferred that above-mentioned lactobacillus-fermented product complex is prepared by the method comprised the following steps：
Streptococcus cremoris, lactic acid leukonid and Bifidobacterium are inoculated in into 30-35 DEG C of progress in lactic acid bacteria nutrient medium respectively to detest
Aerobe fermentation, for example, 35 DEG C of anaerobic fermentations 24 hours, are collected by centrifugation the 5-15% that original volume is concentrated into after zymotic fluid, by three kinds of work
Property lactobacillus-fermented product mix in proportion, then mixed tunning and maize cob meal are pressed into 2-4：1 volume mass ratio
Mixing.
It is preferred that, the lactic acid bacteria nutrient medium can be commercially available lactic acid bacteria nutrient medium, and for example (MRS is cultivated
Base).
It is preferred that, the intestinal villus tissue repair activator includes following parts by weight of component：1-3 parts of citric acid, purple sweet potato powder
2-5 parts, 3-5 parts of pumpkin digested tankage, 15-25 parts of mulberry leaf, 20-25 parts of yam extract, 20-35 parts of Rhizoma Smilacis Glabrae extract, the radix paeoniae rubrathe carries
Take 12-15 parts of thing.
It is further preferred that the intestinal villus tissue repair activator includes following parts by weight of component：3 parts of citric acid, purple
5 parts of potato powder, 5 parts of pumpkin digested tankage, 15 parts of mulberry leaf, 25 parts of yam extract, 35 parts of Rhizoma Smilacis Glabrae extract, 12 parts of red paeonia extract.
It is further preferred that the pumpkin digested tankage is the drying micro mist of pumpkin pulp, after the drying of preferably fresh pumpkin pulp
Comminuted powder, such as be to crush obtained powder after 5-8% by 37 DEG C of constant temperature dryings of pumpkin pulp to moisture.Specifically, at one
In preferred embodiment, the step of preparing the pumpkin digested tankage includes：Fresh pluck after pumpkin removes the peel remove seed is obtained into pumpkin pulp, by south
37 DEG C of constant temperature dryings of melon meat to moisture is 5-8%, crosses 80 mesh pulverizers and is crushed, the micro mist of acquisition is pumpkin digested tankage.
It is further preferred that the yam extract is the ether extract of Chinese yam, specifically, using ether to crushing after
Chinese yam carry out circumfluence distillation after be evaporated the extract being dried to obtain.
It is further preferred that the yam extract is prepared by the method comprised the following steps：Chinese yam is crushed
The ether of 8-12 times of volume is added afterwards, and circumfluence distillation is 3-6 times, such as 5 times, each 1-3 hours, such as 2.5 hours, merges
Extract solution, filtering, is concentrated under reduced pressure, less than 70 DEG C are evaporated acquisition medicinal extract, such as 55 DEG C, medicinal extract are drained at steam through low-temp low-pressure
Reason obtains the powder that water content is 3-5%, as yam extract.
It is further preferred that the yam extract is prepared by the method comprised the following steps：Chinese yam is crushed
Cross 80 eye mesh screens afterwards and be made yam flour, add 10 times of volume ether, circumfluence distillation 5 times, 2.5 hours every time, merge extract solution,
Filter, be concentrated under reduced pressure, 55 DEG C of constant temperature are evaporated acquisition medicinal extract, it is 3- that medicinal extract is drained into steam treatment formation moisture content through low-temp low-pressure
5% solid state powder, the solid state powder obtained as yam extract.
It is further preferred that the Rhizoma Smilacis Glabrae extract is by the way that the residue after smilax extracted by ether is used into n-butanol
Extract is evaporated after extraction.
It is further preferred that the Rhizoma Smilacis Glabrae extract is prepared by the method comprised the following steps：By 1:8-10
W/v ether is added into smilax, be heated to reflux, filter, filtrate is evaporated, is dissolved in water into residue, then with just
Butanol is extracted 1-3 times, is merged extract solution, is washed with water 2-4 times, is evaporated, the material of acquisition is Rhizoma Smilacis Glabrae extract.
It is further preferred that Rhizoma Smilacis Glabrae extract is prepared by the method comprised the following steps：Volume ratio by weight
1:10 add ether into smilax, and 45min is heated to reflux in rotary distillation instrument, filtration, and filtrate is evaporated, according to smilax：
The w/v 1 of water:20 are dissolved in water, and are extracted 2 times with n-butanol, merge extract solution, are washed with water 3 times, are evaporated, acquisition
Material is Rhizoma Smilacis Glabrae extract.
It is further preferred that above-mentioned red paeonia extract is by the residue after the absolute ethyl alcohol extract by the radix paeoniae rubrathe, using chlorine
The extract that is evaporated after imitative extraction is obtained.
It is further preferred that red paeonia extract is prepared by the method comprised the following steps：Volume ratio 1 by weight:
8-10 adds absolute ethyl alcohol into the radix paeoniae rubrathe, is heated to reflux, filtration, and filtrate is evaporated, and residue is dissolved in water, with chloroform recovery 1-3 times,
Merge extract solution, extract and separate 2-4 times is evaporated, and the material of acquisition is red paeonia extract.
It is further preferred that the red paeonia extract is prepared by the method comprised the following steps：Volume by weight
Than 1:8 add absolute ethyl alcohol into the radix paeoniae rubrathe, and 45min is heated to reflux in rotary distillation instrument, filtration, and filtrate is evaporated, residue according to
The radix paeoniae rubrathe：The w/v 1 of water:18 are dissolved in water, then with chloroform recovery 2 times, merge extract solution, and extract and separate 3 times is evaporated,
The material of acquisition is red paeonia extract.
It is preferred that, the multidimensional premix can be commercially available conventional multidimensional premix, the special fresh-water fishes multidimensional of such as glycolylurea
Premix.
It is preferred that, many ore deposit premixes can be commercially available conventional many ore deposit premixes, for example many ore deposits of the special fresh-water fishes of glycolylurea
Premix.
It is preferred that, the endotoxin adsorbent can be conventional endotoxin adsorbent, for example, take off mould montmorillonite.
Applicant has found by the detection for many years to grass carp alimentary canal tissue morphology, special for the grass carp of whole feedstuff feeding
It is not two age grass carps, when average daily feeding rate is more than 3%, different degrees of atrophy or development occurs in rear intestinal segment intestinal villus in grass carp
Bad phenomenon, its probability of happening is up to 45%.The lasting presence of this phenomenon produces bad to intake bait in enteron aisle digestion
Influence, easily causes feed to be arranged outside with the migration in digestion in the case where not digesting and assimilating completely, not only easily causes
Feed coefficient virtual height, also easily increases emptily single jin of grass carp breeding feed use cost, while impaired intestinal villus site easily causes
The generation of grass carp Secondary cases enteritis.Therefore, grass carp is persistently repaired to be damaged intestinal villus or promote intestinal villus grow can be effective
Lifting grass carp feed conversion rate, reduce feed coefficient, reduce Secondary cases enteritis occurrence risk, particularly when grass carp enters high
During the feeding rate stage, effect is more notable.
It is a kind of as present invention offer effectively to repair the ecological feed of grass carp intestinal villus, by repairing grass carp intestines suede
Hair, can effectively improve the feed conversion rate of grass carp, reduce feed coefficient, and reduce grass carp Ctenopharyngodon idellus incidence.
In second aspect, the present invention provides a kind of preparation method of the ecological feed of above-mentioned reparation grass carp intestinal villus.
A kind of method for the ecological feed for repairing grass carp intestinal villus, the component that the feed is included is crushed, is mixed to get.
It is preferred that, a kind of method for the ecological feed for repairing grass carp intestinal villus, the component that the feed is included is crushed, mixed
Close, then extrude cutting after obtain.
Compared with prior art, the present invention has the advantages that：
1st, feed formula of the invention is matched by the protein content and lipid content of optimization, the lactobacillus-fermented production of addition
Thing compound is composited by the fermentate of various active lactic acid bacteria, be intestinal villus grow offer it is necessary trace element and
The various active factor, has good growth regulating effect to grass carp intestinal villus epithelial cell, may additionally facilitate epithelial cell and be damaged
Close connection between autonomous cover reparation afterwards, enhancing intestinal villus epithelial cell；
2nd, the intestinal villus tissue repair activator added in feed of the present invention, rich in various active amino acid and active polysaccharide
Class material, the activation of zymogen factor and cell differentiation promotive factor necessary to being repaired after being also damaged containing a small amount of intestinal tissue, can be strong
Power repairs the atrophy of grass carp intestinal villus or undergrowth than more serious tissue site, can not only maintain intestinal villus Stability Analysis of Structures, also
Intestinal villus can be promoted to grow, bait conversion absorption efficiency is improved；
3rd, the synergy of the invention by lactobacillus-fermented product complex and intestinal villus tissue repair activator, to grass
The reparation of fish intestinal villus has unexpected effect, then has to the growth of grass carp, feed conversion rate, enteritis incidence of disease aspect
There is prominent wholesome effect.
Embodiment
The present invention is further described below in conjunction with specific embodiment.
Embodiment 1：
A kind of ecological feed for repairing grass carp intestinal villus, by weight, constituent is as follows：15 parts of chicken meal, squid
5 parts of 2 parts of cream, 20 parts of fermented bean dregs, 30 parts of rapeseed meal, 10 parts of expanded cotton dregs, distiller's dried grain and its DDGS (DDGS), 8 parts of wheat, sauce
1 part of the dregs of fat, 1 part of soya-bean oil, 1.7 parts of calcium dihydrogen phosphate, 0.2 part of Choline Chloride, 0.2 part of multidimensional premix, many ore deposit premixes 0.2
Part, endotoxin adsorbent take off mould 0.2 part of montmorillonite, 4 parts of flour, 0.5 part of lactobacillus-fermented product complex, intestinal villus tissue repair
1 part of activator.The component that the feed is included is crushed, mixing, is obtained after extruding cutting.
Wherein, the component of lactobacillus-fermented product complex is：45 parts of streptococcus cremoris tunning, lactic acid leukonid
35 parts of tunning, 20 parts of bifidobacterium fermentation product, the preparation method of the tunning compound is：Respectively by cheese hammer
Bacterium, lactic acid leukonid and Bifidobacterium are inoculated in 35 DEG C of progress anaerobic fermentation 24 hours in lactic acid bacteria nutrient medium, centrifugation
The 10% of original volume is concentrated into after collecting zymotic fluid, three kinds of active lactobacillus fermented products are mixed in proportion, then by after mixing
Tunning and maize cob meal press 2：1 volume mass is than mixing.
Wherein, the component of intestinal villus tissue repair activator is：3 parts of citric acid, 5 parts of purple sweet potato powder, 5 parts of pumpkin digested tankage, mulberry
15 parts of leaf, 25 parts of yam extract, 35 parts of Rhizoma Smilacis Glabrae extract, 12 parts of red paeonia extract.Wherein, pumpkin digested tankage is prepared as：
Fresh pluck after pumpkin removes the peel remove seed is obtained into pumpkin pulp, is 5-8% by 37 DEG C of constant temperature dryings of pumpkin pulp to moisture, crosses 80 mesh pulverizers
Crushed, the powder of acquisition is pumpkin digested tankage.Wherein, the preparation method of yam extract is as follows：Mistake after Chinese yam is crushed
Yam flour is made in 80 eye mesh screens, adds 10 times of volume ether, and circumfluence distillation 5 times 2.5 hours every time, merges extract solution, mistake
Filter, be concentrated under reduced pressure, 55 DEG C of constant temperature are evaporated acquisition medicinal extract, it is 3-5% that medicinal extract is drained into steam treatment formation moisture content through low-temp low-pressure
Solid state powder, the solid state powder obtained as yam extract；Wherein, the preparation method of Rhizoma Smilacis Glabrae extract is as follows：By weight
Measure volume ratio 1:10 add ether into smilax, and 45min is heated to reflux in rotary distillation instrument, and filtration, filtrate is evaporated, according to
Smilax：The w/v 1 of water:20 are dissolved in water, and are extracted 2 times with n-butanol, merge extract solution, are washed with water 3 times, steam
Dry, the material of acquisition is Rhizoma Smilacis Glabrae extract；Wherein, the preparation method of red paeonia extract is as follows：Volume ratio 1 by weight:8 to
Absolute ethyl alcohol is added in the radix paeoniae rubrathe, 45min is heated to reflux in rotary distillation instrument, is filtered, filtrate is evaporated, and residue is according to the radix paeoniae rubrathe：Water
W/v 1:18 are dissolved in water, then with chloroform recovery 2 times, merge extract solution, and extract and separate 3 times is evaporated, the thing of acquisition
Matter is red paeonia extract.
Example 2
A kind of ecological feed of grass carp intestinal villus growth-promoting elongated, by weight, constituent is as follows：13 parts of chicken meal,
3 parts of squid liver paste, 18 parts of fermented bean dregs, 35 parts of rapeseed meal, 7 parts of expanded cotton dregs, distiller's dried grain and its 4 parts of DDGS (DDGS), wheat 8
Part, 1 part of soy sauce residues, 1 part of soya-bean oil, 1.7 parts of calcium dihydrogen phosphate, 0.2 part of Choline Chloride, 0.2 part of multidimensional premix, many ore deposit premixes
0.2 part, 0.2 part of endotoxin adsorbent, 4 parts of flour, 0.3 part of lactobacillus-fermented product complex, intestinal villus tissue repair activator
1.2 parts.The component that the feed is included is crushed, mixing, is alternatively obtained after extruding cutting.
Wherein, the component of lactobacillus-fermented product complex is：40 parts of streptococcus cremoris tunning, lactic acid leukonid
35 parts of tunning, 25 parts of bifidobacterium fermentation product, the preparation method of the tunning compound is：Respectively by cheese hammer
Bacterium, lactic acid leukonid and Bifidobacterium are inoculated in 35 DEG C of anaerobic fermentations of progress in lactic acid bacteria nutrient medium, and hair is collected by centrifugation
The 15% of original volume is concentrated into after zymotic fluid, three kinds of active lactobacillus fermented products are mixed in proportion, then by mixed fermentation
Product presses 4 with maize cob meal：1 volume mass is than mixing.
Wherein, the component of intestinal villus tissue repair activator is：3 parts of citric acid, 5 parts of purple sweet potato powder, 5 parts of pumpkin digested tankage, mulberry
20 parts of leaf, 20 parts of yam extract, 32 parts of Rhizoma Smilacis Glabrae extract, 15 parts of red paeonia extract.Wherein, pumpkin digested tankage, Chinese yam are extracted
Thing, Rhizoma Smilacis Glabrae extract, red paeonia extract preparation as described in Example 1.
Test example 1
Using the grass carp of embodiment 1, ecological feed prepared by embodiment 2 and identical nutrition class commercially
Pellet carries out grass carp and feeds experiment, and numbering is 1 group, 2 groups and control group respectively.In the healthy grass carp temporarily supported from 3000 tails with
Machine selects 1800 tails, and grass carp average out to 100g ± 5g, feed all carries out dispensing by 2.5% average daily feeding rate.
The grass carp pellet of identical nutrition class its trophic component commercially is：Crude protein 28%, fat
6%th, crude fibre 18%, lysine 2.5%, calcium 0.5-4%, coarse ash 12%.
Intestinal villus tissue conformal analysis, intestinal villus length measurment and goblet cell are carried out to 1 group, 2 groups, the grass carp of control group
Quantitative measurement.
Intestinal villus tissue conformal analysis：Stochastical sampling is carried out in experiment the 30th, 60 days grass carps to each group respectively, every group is adopted
Collect the tail of grass carp 5, by taking middle intestines sample after dissection respectively, by midgut tissue by fixing, being put into full-automatic slicing machine, through leaching
The making of histotomy is completed after wax, embedding, dehydration, dyeing, dehydration, transparent and mounting step, observes different in microscope
Group grass carp midgut tissue form, com-parison and analysis intestinal villus integrality, intestinal villus epithelial cell, goblet cell and chyle are vasculolymphatic
Morphological integrity.
Intestinal villus length measurment：The villus length of different groups of intestinal tissue samples is measured by IPP6.0 softwares, and
Significance difference analysis is carried out by SPSS15 softwares.
Goblet cell quantitative measurement：Different groups of intestinal tissue fine hair goblet cells are counted by IPP6.0 softwares.
Analysis and measurement result are as follows：
(1) intestinal villus histomorphometric analysis
Observation analysis understands, during experiment the 0th day, 1 group, 2 groups, the grass carp midgut tissue morphological integrity of control group it is good,
Locally there is the phenomenon of epithelial cell shedding in middle intestinal villus, and inflammatory cell infiltration is not found, and free red blood cell infiltration is not found.It is whole
Body analyze 1 group, 2 groups, the grass carp midgut tissue form of control group meet normal histology's category.
When testing the 30th day, 1 group, 2 groups of grass carp midgut tissue morphological integrity is good, middle intestinal villus topical epithelial cell
The phenomenon come off obtains significant improvement, without any visible inflammatory lesion or hemorrhagic lesions, meets normal histology's category.It is right
There is obvious fine hair end according to group grass carp midgut tissue to come off, middle lacteal vessel partial structurtes are damaged, and intestinal wall muscle layer goes out current situation
Portion's lytic necrosis, thus infers, control group grass carp occurs with the fine hair necrotic lesion of locality, with obvious hypofunction
Process.
When testing the 60th day, 1 group, 2 groups of grass carp midgut tissue morphological integrities it is good, middle intestinal villus topical epithelial cell takes off
The phenomenon fallen obtains significant improvement, without any visible inflammatory lesion or hemorrhagic lesions, meets normal histology's category.To group
There is obvious fine hair end and come off in grass carp midgut tissue, and middle lacteal vessel partial structurtes are damaged, and intestinal wall muscle layer occurs local molten
The necrosis of solution property, thus infers, control group grass carp occurs with the fine hair necrotic lesion of locality, with obvious hypofunction
Journey.
(2) intestinal villus length measurment result
In different time, the middle intestinal villus length to three groups is measured, as a result as shown in table 1.
Table 1：
As can be seen from the above table, when testing the 0th day, three test group grass carp midgut tissue intestinal villus differences in length are not notable
(p>0.05)；When extending to 30 days with Feeding time, intestinal villus length is significantly higher than control group (p in 1 group and 2 groups of grass carp<
0.05)；When Feeding time extends to 60 days, intestinal villus length is significantly higher than control group (p in 1 group and 2 groups of grass carp<
0.05).As can be seen here, intestinal digestion suction in grass carp intestinal villus length, increase grass carp can be obviously improved by feeding feed of the present invention
Contact surface area is received, promotes the enhancing of digestive function.
(3) intestinal villus goblet cell count results
Goblet cell is the one of intestinal villus important composition point, how much close phases of health degree with intestinal villus of its quantity
Close, the power with fish body digestive function is also closely related.Therefore, the quantity of goblet cell has reflected intestinal villus to a certain extent
Health degree.Grass carp midgut tissue fine hair both sides goblet cell quantity result never with timing node collection is as shown in table 2.
Table 2:
From table 2 it can be seen that when testing the 0th day, three test group grass carp midgut tissue intestinal villus both sides goblet cell numbers
Measure difference not significantly (p>0.05)；When extending to 30 days with Feeding time, intestinal villus both sides cup-shaped in 1 group and 2 groups of grass carp
Cell quantity is significantly higher than control group (p<0.05)；When Feeding time extends to 60 days, intestinal villus in 1 group and 2 groups of grass carp
Both sides goblet cell quantity is significantly higher than control group (p<0.05).As can be seen here, feeding feed of the present invention can be significant
Promote intestinal digestion absorbing function in the Proliferation, Differentiation of grass carp intestinal villus both sides goblet cell, increase grass carp.
Influence of the test example 2 to growth performance index of correlation
Using the grass carp of embodiment 1, ecological feed prepared by embodiment 2 and identical nutrition class commercially
Pellet carries out grass carp and feeds experiment, and numbering is 1 group, 2 groups and control group respectively.In the healthy grass carp temporarily supported from 3000 tails with
Machine selects 1800 tails, and grass carp average out to 100g ± 5g, feed all carries out dispensing by 2.5% average daily feeding rate.
The grass carp pellet of identical nutrition class its trophic component commercially is：Crude protein 28%, fat
6%th, crude fibre 18%, lysine 2.5%, calcium 0.5-4%, coarse ash 12%.
In test period, each group grass carp is weighed at experiment the 0th, 61 days respectively, with reference to the net weight for feeding feed
Amount, calculates each group grass carp feed coefficient, rate of body weight gain, as a result as shown in table 3 according to below equation.
Feed coefficient=always feed net gain (kg) during feed relative (kg)/grass carp experiment
Rate of body weight gain=(experiment latter stage grass carp weight (kg)-experiment grass carp weight at initial stage (kg))/60 days
Table 3：
| Group | Feed coefficient | Rate of body weight gain |
| 1 group | 1.23±0.01a | 10.32±0.02b |
| 2 groups | 1.22±0.01a | 10.33±0.03b |
| Control group | 1.41±0.01b | 9.23±0.01a |
As can be known from Table 3, in the test period, 1 group and 2 groups of feed coefficient and rate of body weight gain are significantly higher than control group (p<
0.05).As can be seen here, compared with common grass carp feed, feeding ecological feed prepared by the embodiment of the present invention 1, embodiment 2 can be with
Grass carp feed coefficient is effectively reduced, grass carp rate of body weight gain is improved.
Test example 3
Using the grass carp of embodiment 1, ecological feed prepared by embodiment 2 and identical nutrition class commercially
Pellet carries out grass carp and feeds experiment, and numbering is 1 group, 2 groups and control group respectively.In the healthy grass carp temporarily supported from 3000 tails with
Machine selects 1800 tails, and grass carp average out to 100g ± 5g, feed all carries out dispensing by 2.5% average daily feeding rate.
The grass carp pellet of identical nutrition class its set of tags commercially is grouped into：Crude protein 28%,
Fat 6%, crude fibre 18%, lysine 2.5%, calcium 0.5-4%, coarse ash 12%.
Within the test period, continuous observation and the dying grass carp of salvaging each test group morbidity, according to morbidity grass carp morbidity disease
The survival rate and the enteritis death rate of grass carp between shape, Pathogen identification result, statistics different tests group, as a result as shown in table 4.
Table 4
| Group | Survival rate | Overall mortality rate | The enteritis death rate |
| 1 group | 100% | 0 | 0 |
| 2 groups | 100% | 0 | 0 |
| Control group | 91% | 4% | 3% |
As can be known from the results of Table 4, there is zero morbidity zero death in continuous 60 days, 1 group and 2 groups of grass carps, and control group grass carp
The death rate 4%, it is 3% to range the dead ratio of enteritis.As can be seen here, compared with common grass carp feed, feed of the invention real
The death rate of grass carp Ctenopharyngodon idellus can be effectively reduced by applying example 1, the ecological feed of the preparation of embodiment 2.
Summary result of the test understands that compared with control group, 1 group and 2 groups can be obviously improved intestinal villus in grass carp
Length, increase intestinal villus both sides goblet cell quantity have significant effect, simultaneously for the complete of maintenance midgut tissue form
Property also have stable effect, be also beneficial to reduce enteritis onset risk.Compared to the using effect of commercially available same class commercial feed,
There is ecological feed prepared by the present invention significant repairing effect and enteritis to occur preventive effect to grass carp intestinal villus, also can be notable
Reduction feed coefficient and improve rate of body weight gain.
The above, is only the preferably specific embodiment of the present invention, but protection scope of the present invention is not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Design is subject to equivalent or change, should all cover within the scope of the present invention.
Claims (10)
1. a kind of ecological feed for repairing grass carp intestinal villus, it is characterised in that include the component of following parts by weight：Chicken meal 10-
17 parts, 1-3 parts of squid liver paste, 12-22 parts of fermented bean dregs, 20-35 parts of rapeseed meal, expanded cotton dregs 5-12 parts, distiller's dried grain and its DDGS
5-15 parts, it is 8-18 parts of wheat, 1-4 parts of soy sauce residues, 1-2 parts of soya-bean oil, 1-3 parts of calcium dihydrogen phosphate, 0.1-0.2 parts of Choline Chloride, many
Tie up 0.15-0.2 parts of premix, 0.15-0.2 parts of many ore deposit premixes, 0.15-0.2 parts of endotoxin adsorbent, 4-8 parts of flour, lactic acid bacteria
0.1-0.5 parts of tunning compound, 1-3 parts of intestinal villus tissue repair activator.
2. a kind of ecological feed for repairing grass carp intestinal villus according to claim 1, it is characterised in that include following weight
The component of part：15 parts of chicken meal, 2 parts of squid liver paste, 20 parts of fermented bean dregs, 30 parts of rapeseed meal, 10 parts of expanded cotton dregs, distiller's dried grain and its can
Molten 5 parts of thing, 8 parts of wheat, 1 part of soy sauce residues, 1 part of soya-bean oil, 1.7 parts of calcium dihydrogen phosphate, 0.2 part of Choline Chloride, multidimensional premix 0.2
Part, 0.2 part of many ore deposit premixes, 0.2 part of endotoxin adsorbent, 4 parts of flour, 0.5 part of lactobacillus-fermented product complex, intestinal villus group
Knit 1 part of activator of reparation.
3. a kind of ecological feed for repairing grass carp intestinal villus according to claim 1, it is characterised in that include following weight
The component of part：13 parts of chicken meal, 3 parts of squid liver paste, 18 parts of fermented bean dregs, 35 parts of rapeseed meal, 7 parts of expanded cotton dregs, distiller's dried grain and its can
Molten 4 parts of thing, 8 parts of wheat, 1 part of soy sauce residues, 1 part of soya-bean oil, 1.7 parts of calcium dihydrogen phosphate, 0.2 part of Choline Chloride, multidimensional premix 0.2
Part, 0.2 part of many ore deposit premixes, 0.2 part of endotoxin adsorbent, 4 parts of flour, 0.3 part of lactobacillus-fermented product complex, intestinal villus group
Knit 1.2 parts of activator of reparation.
4. a kind of ecological feed of reparation grass carp intestinal villus according to any one of claim 1-3, it is characterised in that breast
Acid bacteria fermentation product complex includes following parts by weight of component：40-45 parts of streptococcus cremoris tunning, lactic acid leukonid hair
30-35 parts of ferment product, 20-25 parts of bifidobacterium fermentation product.
5. a kind of ecological feed for repairing grass carp intestinal villus according to claim 4, it is characterised in that the lactic acid bacteria hair
Ferment product complex is prepared by the method comprised the following steps：Respectively by streptococcus cremoris, lactic acid leukonid and double
Discrimination bacillus is inoculated in 30-35 DEG C of anaerobic fermentation of progress in lactic acid bacteria nutrient medium, is collected by centrifugation after zymotic fluid and is concentrated into substance
Long-pending 5-15%, after three kinds of active lactobacillus fermented products are mixed, then by mixed tunning and maize cob meal by 2-
4：1 volume mass is than mixing.
6. a kind of ecological feed of reparation grass carp intestinal villus according to any one of claim 1-3, it is characterised in that intestines
Chorionic villi repairs activator and includes following parts by weight of component：1-3 parts of citric acid, 2-5 parts of purple sweet potato powder, 3-5 parts of pumpkin digested tankage, mulberry
15-25 parts of leaf, 20-25 parts of yam extract, 20-35 parts of Rhizoma Smilacis Glabrae extract, 12-15 parts of red paeonia extract.
7. a kind of ecological feed for repairing grass carp intestinal villus according to claim 6, it is characterised in that the Chinese yam is extracted
Thing is prepared by the method comprised the following steps：8-12 times of ether of addition after Chinese yam is crushed, circumfluence distillation, 3-6 times,
1-3 hours every time, merge extract solution, filtering is concentrated under reduced pressure, less than 70 DEG C are evaporated acquisition medicinal extract, and medicinal extract is taken out through low-temp low-pressure
Dry saturated steam processing obtains the powder that water content is 3-5%, as yam extract.
8. a kind of ecological feed for repairing grass carp intestinal villus according to claim 6, it is characterised in that the smilax is carried
Thing is taken to be prepared by the method comprised the following steps：By 1:8-10 w/v adds ether into smilax, plus
Heat backflow, filtration, filtrate is evaporated, is dissolved in water into residue, then is extracted 1-3 times with n-butanol, merges extract solution, is washed with water
2-4 times, it is evaporated, the material of acquisition is Rhizoma Smilacis Glabrae extract.
9. a kind of ecological feed for repairing grass carp intestinal villus according to claim 6, it is characterised in that red paeonia extract leads to
The method comprised the following steps is crossed to prepare：Volume ratio 1 by weight:8-10 adds absolute ethyl alcohol into the radix paeoniae rubrathe, is heated to reflux,
Filtration, filtrate is evaporated, and residue is dissolved in water, and with chloroform recovery 1-3 time, merges extract solution, extract and separate 2-4 times is evaporated, acquisition
Material be red paeonia extract.
10. a kind of a kind of side of the ecological feed of the reparation grass carp intestinal villus prepared according to any one of claim 1-9
Method, it is characterised in that the component for including the feed is crushed, mixing, alternatively obtains the ecological feed after extruding cutting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710200953.6A CN106954761A (en) | 2017-03-30 | 2017-03-30 | A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710200953.6A CN106954761A (en) | 2017-03-30 | 2017-03-30 | A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106954761A true CN106954761A (en) | 2017-07-18 |
Family
ID=59471836
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710200953.6A Pending CN106954761A (en) | 2017-03-30 | 2017-03-30 | A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106954761A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109198266A (en) * | 2018-09-27 | 2019-01-15 | 清远海贝生物技术有限公司 | A kind of compound premix and its preparation method and application increasing strong fish enteron aisle |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101983582A (en) * | 2010-11-24 | 2011-03-09 | 广州市海维饲料有限公司 | Traditional Chinese medicine microecological preparation for preventing and treating enteritis of grass carp and preparation method thereof |
| CN102150757A (en) * | 2011-02-14 | 2011-08-17 | 北京伟嘉人生物技术有限公司 | Aquatic product feed additive composition, preparation method and application thereof |
| WO2013167659A1 (en) * | 2012-05-09 | 2013-11-14 | Evonik Industries Ag | Animal feed additive, containing l-amino acid, in the form of a granular material based on fermentation broth and method for production |
| CN103550354A (en) * | 2013-10-30 | 2014-02-05 | 福建农林大学 | Compound stomach-invigorating and intestine-treating granules for fishes and preparation method thereof |
| CN105076713A (en) * | 2015-09-30 | 2015-11-25 | 通威股份有限公司 | Composite feed capable for improving intestinal health of tilapia and preparation method of composite feed |
| CN105166568A (en) * | 2015-10-12 | 2015-12-23 | 清远海贝生物技术有限公司 | Micro-ecological feed for preventing bacillary enteritis of grass carps |
| CN105795111A (en) * | 2016-03-17 | 2016-07-27 | 青岛根源生物技术集团有限公司 | Intestinal barrier function improving compound micro-ecological feed additive and application thereof |
-
2017
- 2017-03-30 CN CN201710200953.6A patent/CN106954761A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101983582A (en) * | 2010-11-24 | 2011-03-09 | 广州市海维饲料有限公司 | Traditional Chinese medicine microecological preparation for preventing and treating enteritis of grass carp and preparation method thereof |
| CN102150757A (en) * | 2011-02-14 | 2011-08-17 | 北京伟嘉人生物技术有限公司 | Aquatic product feed additive composition, preparation method and application thereof |
| WO2013167659A1 (en) * | 2012-05-09 | 2013-11-14 | Evonik Industries Ag | Animal feed additive, containing l-amino acid, in the form of a granular material based on fermentation broth and method for production |
| CN103550354A (en) * | 2013-10-30 | 2014-02-05 | 福建农林大学 | Compound stomach-invigorating and intestine-treating granules for fishes and preparation method thereof |
| CN105076713A (en) * | 2015-09-30 | 2015-11-25 | 通威股份有限公司 | Composite feed capable for improving intestinal health of tilapia and preparation method of composite feed |
| CN105166568A (en) * | 2015-10-12 | 2015-12-23 | 清远海贝生物技术有限公司 | Micro-ecological feed for preventing bacillary enteritis of grass carps |
| CN105795111A (en) * | 2016-03-17 | 2016-07-27 | 青岛根源生物技术集团有限公司 | Intestinal barrier function improving compound micro-ecological feed additive and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109198266A (en) * | 2018-09-27 | 2019-01-15 | 清远海贝生物技术有限公司 | A kind of compound premix and its preparation method and application increasing strong fish enteron aisle |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101912066B (en) | Meat sheep feed containing traditional Chinese medicine extraction residue components and application thereof | |
| CN102204639B (en) | Chinese traditional medicine feed for feeding beef cattle and method for preparing same | |
| CN103070306A (en) | Meat rabbit feed and preparation method thereof | |
| CN103109972A (en) | Method for processing inapplicable tobacco leaves by musca domestica larva to prepare biological protein feed | |
| CN107319148A (en) | A kind of growth promotion ecological immunity type sika deer special feed and preparation method thereof | |
| CN101914472A (en) | Eucommia leaf extract and preparation method and application thereof | |
| CN105053602A (en) | Mulberry pomace chicken feed and preparation method thereof | |
| CN102204625A (en) | Chinese medicine forage for feeding cow, and preparation method thereof | |
| CN105104756B (en) | A kind of mushroom bran protein peptides of alternative fish meal and preparation method thereof and the application in aquaculture | |
| CN102987137A (en) | Compound fattening feed for mutton sheep and preparation method of feed | |
| CN108450411A (en) | A kind of coypu cultural method | |
| CN103947871B (en) | A kind of meat goose mixed feed and preparation method thereof | |
| CN104522387A (en) | Heat-clearing beef cattle feed containing white poplar barks and preparation method of heat-clearing beef cattle feed | |
| CN106720936A (en) | A kind of fermented feed additive containing dalbergia wood leaf composition and its preparation method and application | |
| CN109329614A (en) | A kind of beef cattle fully mixed diet fermented feed and preparation method thereof | |
| CN108935998A (en) | Mutton sheep fully mixed diet and its preparation method and application | |
| CN106954761A (en) | A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof | |
| CN107373045A (en) | One kind prevention leukodermal solid feed of lobster and preparation method thereof | |
| CN111296659A (en) | Efficient grain-saving immune type fermented compound feed for weaned piglets and preparation method thereof | |
| CN106721194B (en) | Additive for increasing beef intramuscular fat and flavor | |
| CN107712386A (en) | Lay eggs feed for ostrich and preparation method thereof | |
| CN107691772A (en) | Swimming crab feed and preparation method thereof | |
| CN103947833B (en) | A kind of egg goose mixed feed and preparation method thereof | |
| CN112088839B (en) | Feeding method for improving meat quality and flavor of cage-cultured laying ducks | |
| CN109043208A (en) | Utilize the method for salt-soda soil plant feed additive feeding laying hen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170718 |
|
| RJ01 | Rejection of invention patent application after publication |