CN112655816A - Feed additive for preventing and treating bovine frog ascites disease and preparation method and application thereof - Google Patents

Feed additive for preventing and treating bovine frog ascites disease and preparation method and application thereof Download PDF

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CN112655816A
CN112655816A CN202011563424.0A CN202011563424A CN112655816A CN 112655816 A CN112655816 A CN 112655816A CN 202011563424 A CN202011563424 A CN 202011563424A CN 112655816 A CN112655816 A CN 112655816A
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涂永芹
贠彪
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Guangdong Hinter Bio Technology Group Co ltd
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

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Abstract

The invention relates to a feed additive for preventing and treating bovine frog ascites disease, a preparation method and application thereof, wherein the feed additive comprises the following components in parts by weight: 15-30 parts of fructus forsythiae, 15-30 parts of radix bupleuri, 1-2 parts of allicin, 2-4 parts of bile acid, 15-25 parts of lactobacillus acidophilus freeze-dried powder, 15-25 parts of bacillus subtilis freeze-dried powder and 10-20 parts of corn starch. According to the invention, the allicin is compatible with the forsythia and the radix bupleuri to enhance the drug effect, and the combination of the bile acid, the forsythia and the radix bupleuri can effectively improve the liver function and reduce the probability of liver pathological changes; on the other hand, the lactobacillus acidophilus and the bacillus subtilis are adopted, so that the regulation and inhibition of flora in digestive tracts such as intestinal tracts and the like are facilitated, pathogenic bacteria in a water body are inhibited, the absorption of animals on nutrient substances is promoted, and the feed conversion rate is improved; finally, the combination of traditional Chinese medicines and beneficial bacteria has no side effect, can prevent and treat ascites disease, reduce feed coefficient and improve weight gain rate, and the combination of the traditional Chinese medicines and the beneficial bacteria obviously enhances the effects and improves economic benefit.

Description

Feed additive for preventing and treating bovine frog ascites disease and preparation method and application thereof
Technical Field
The invention relates to a feed additive, in particular to a feed additive for preventing and treating bovine frog ascites disease and a preparation method and application thereof.
Background
Bullfrog (Rana catesbeian) belongs to Amphinaceae, Anhula, Ranidae, and is a large edible frog because it sounds like a cow. The bullfrog is originally produced in North America, Mexico and other places, is introduced into China from Gouba in 1959, is a meat food with high protein, low fat and low cholesterol, has delicious taste, high growth speed and strong environmental adaptability, and has wide consumption market at home and abroad. In 2019, the breeding yield of bullfrogs in China is close to 50 million tons, the direct sale yield exceeds 50 million yuan per year, and the bullfrogs become important famous and special varieties for aquaculture in China.
In the process of breeding bullfrogs, ascites diseases are easy to occur in high-temperature seasons, and are very common particularly under the conditions of water quality deterioration, high stocking density and poor management. The main symptoms of the bovine frog ascites disease are: the abdomen is enlarged, a large amount of ascites and liver enlargement are dissected; in severe cases, the diseased frog has weakness in limbs, anorexia, large amount of yellowish or reddish ascites, and congestion and inflammation of the intestinal and gastric walls. The pathogeny of the bullfrog ascites disease is mainly aeromonas hydrophila, has strong infectivity, is easy to erupt in the whole pool, causes the death of bullfrogs in a large amount in a short time, and causes serious economic loss.
Generally, farmers use sulfonamides or sars drugs for prevention and treatment, however, the existing drugs have no special effect on ascites disease, and meanwhile, due to the problems of drug resistance, drug residue influencing meat quality, high drug cost, inconvenient operation and the like, the industry needs a green feed additive capable of replacing antibiotics to solve the problems.
Disclosure of Invention
Aiming at the defects, the invention provides the feed additive, which is used for preventing and treating bullfrog ascites disease in the breeding process, reducing morbidity and mortality and improving the breeding benefit.
The invention achieves the above purposes through the following scheme:
in a first aspect, the invention provides a feed additive for preventing and treating bovine frog ascites disease, which comprises the following components in parts by weight: 15-30 parts of fructus forsythiae, 15-30 parts of radix bupleuri, 1-2 parts of allicin, 2-4 parts of bile acid, 15-25 parts of Lactobacillus acidophilus (Lactobacillus acidophilus) freeze-dried powder, 15-25 parts of Bacillus subtilis freeze-dried powder and 10-20 parts of corn starch.
In a preferred embodiment, the feed additive for preventing and treating the bovine frog ascites disease comprises the following components in parts by weight: 15 parts of fructus forsythiae, 30 parts of radix bupleuri, 2 parts of allicin, 2 parts of bile acid, 15 parts of lactobacillus acidophilus freeze-dried powder, 25 parts of bacillus subtilis freeze-dried powder and 11 parts of corn starch.
In a preferred embodiment, the feed additive for preventing and treating the bovine frog ascites disease comprises the following components in parts by weight: 30 parts of fructus forsythiae, 15 parts of radix bupleuri, 1 part of allicin, 4 parts of bile acid, 25 parts of lactobacillus acidophilus freeze-dried powder, 15 parts of bacillus subtilis freeze-dried powder and 10 parts of corn starch.
In a preferred embodiment, the feed additive for preventing and treating the bovine frog ascites disease comprises the following components in parts by weight: 20 parts of fructus forsythiae, 20 parts of radix bupleuri, 1 part of allicin, 2 parts of bile acid, 20 parts of lactobacillus acidophilus freeze-dried powder, 20 parts of bacillus subtilis freeze-dried powder and 17 parts of corn starch.
Preferably, the bile acid comprises 20% of hyocholic acid, 60% of hyodeoxycholic acid and 20% of chenodeoxycholic acid by weight percentage.
Preferably, the number of viable bacteria of the lactobacillus acidophilus freeze-dried powder after resuscitation is not less than 1 multiplied by 109cfu/g。
Preferably, the bacillus subtilis freeze-dried powder is compoundedThe number of viable bacteria after threatened is not less than 1 × 109cfu/g。
The forsythia and the radix bupleuri used in the invention are traditional Chinese herbal medicines, have broad-spectrum antibacterial action, can effectively prevent and control bacterial infection, do not generate drug resistance, have no residue and do not influence the meat quality of bullfrogs. Moreover, the bullfrog ascites is often complicated with liver lesion, and the forsythia and the bupleurum have obvious liver protection effects, can relieve the degeneration and necrosis of liver cells, and can promote the liver glycogen content to recover to normal;
the allicin used in the invention has wide pharmacological activity and broad antibacterial spectrum, has better inhibiting effect on gram-positive bacteria, gram-negative bacteria and fungi, and can enhance the drug effect by being reasonably compatible with Chinese herbal medicines of fructus forsythiae and radix bupleuri; meanwhile, the allicin has better food calling effect on aquatic animals and can promote the intake of the feed containing Chinese herbal medicines.
The bile acid is the main component of endogenous bile of animals, can dredge biliary tract, effectively decompose and remove bacterial endotoxin, mycotoxin and the like accumulated in liver and intestinal tract, and can repair liver and gall injury; the bile acid can be used together with liver-protecting Chinese herbal medicines fructus forsythiae and bupleuri radix to effectively improve liver function and reduce liver pathological change probability.
After entering the intestinal tract, lactobacillus acidophilus can release lactic acid and antibiotics such as acidophilic lactobacillus, acidophilic lactobacillus and lactein, and can generate antagonistic action on intestinal pathogenic bacteria; meanwhile, lactobacillus acidophilus is excreted into water through excrement to form dominant bacteria, and pathogenic bacteria in water can be competitively inhibited.
The bacillus subtilis also has the function of competitively inhibiting the colonization or proliferation of other external pathogenic microorganisms in the digestive tract; in addition, the bacillus subtilis grows and breeds in animal intestinal tracts, can generate various nutrient substances such as vitamins, amino acids, growth promotion factors and the like to promote animal growth, and meanwhile, the bacillus subtilis also has stronger activities of protease, lipase and amylase, can promote the absorption of the nutrient substances by animals, and improves the conversion efficiency of the feed.
In a second aspect, the invention provides a method for preparing the feed additive for preventing and treating bovine frog ascites disease, which comprises the following steps:
(1) pulverizing fructus forsythiae and bupleuri radix respectively, and sieving;
(2) mixing sieved fructus forsythiae and bupleuri radix with garlicin, bile acid, Lactobacillus acidophilus lyophilized powder, Bacillus subtilis lyophilized powder and corn starch, and stirring to obtain mixture;
(3) and (3) performing secondary crushing on the mixture obtained in the step (2), sieving, and uniformly stirring the sieved mixture to obtain the feed additive.
In a preferred embodiment, a method for preparing the feed additive for preventing and treating bovine frog ascites disease comprises the following steps:
(1) pulverizing fructus forsythiae and bupleuri radix respectively, and sieving with 40 mesh sieve;
(2) mixing fructus forsythiae and bupleuri radix sieved by a 40-mesh sieve with garlicin, bile acid, lactobacillus acidophilus lyophilized powder, bacillus subtilis lyophilized powder and corn starch, and stirring uniformly to obtain a mixture;
(3) and (3) performing secondary crushing on the mixture obtained in the step (2), sieving by using a 100-mesh sieve, and uniformly stirring the sieved mixture to obtain the feed additive.
In a third aspect, the invention provides a feed additive prepared by the preparation method.
In a fourth aspect, the invention provides an application of the feed additive for preventing and treating the bovine frog ascites disease, wherein the additive is added into the compound feed of the bovine frog in an amount of 0.5-2% by weight.
In a preferred embodiment, the feed additive for preventing and treating the bovine frog ascites disease is added into the compound feed of the bullfrog in an amount of 1 percent by weight.
The feed additive for preventing and treating the bullfrog ascites disease is used for preventing the bullfrog ascites disease and/or treating the bullfrog ascites disease, and/or increasing the weight gain rate of the bullfrog, and/or reducing the feed coefficient, and/or reducing the death rate including the death rate caused by the bullfrog ascites disease.
Compared with the prior art, the invention has the beneficial effects that:
the forsythia and the radix bupleuri with the liver protection function have the antibacterial function and the allicin for attracting food, the allicin is matched with the forsythia and the radix bupleuri to enhance the drug effect, and the bile acid, the forsythia and the radix bupleuri are used together to more effectively improve the liver function and reduce the probability of liver pathological change; on the other hand, the lactobacillus acidophilus and the bacillus subtilis are adopted, so that the regulation and inhibition of flora in digestive tracts such as intestinal tracts are facilitated, pathogenic bacteria in water are inhibited, animals are promoted to absorb nutrient substances, and the feed conversion rate is improved; finally, the invention adopts the combination of traditional Chinese medicines and beneficial bacteria, has no side effect, can prevent and treat the ascites disease, reduces the feed coefficient, improves the weight gain rate and the like, and the combination of the traditional Chinese medicines and the beneficial bacteria obviously enhances the effects and greatly improves the economic benefit.
Drawings
FIG. 1 is a graph comparing the average daily mortality in comparative test example 2.
FIG. 2 is a graph comparing the average daily mortality in comparative test example 3.
Detailed Description
The present invention is further illustrated by the following specific examples.
The formula of the common bullfrog compound feed used in the invention is as follows (in parts by weight): 16 parts of domestic semi-defatted fish meal, 10 parts of American chicken meal, 8 parts of domestic pork meal, 5 parts of soybean protein concentrate, 30 parts of soybean meal, 23 parts of high gluten flour, 1 part of refined fish oil, 3 parts of soybean oil, 2 parts of monocalcium phosphate, 1.5 parts of vitamin and mineral salt premix, 0.4 part of choline chloride, 0.05 part of antioxidant and 0.05 part of mildew preventive.
The content of crude protein in the common bullfrog compound feed is 42.7 percent, the content of crude fat is 8.8 percent, and the available energy value is 19.5 kJ/g.
Wherein, the antioxidant adopts ethoxyquinoline.
Wherein, the mildew preventive adopts propionic acid type mildew preventive.
Wherein the vitamin and mineral salt premix comprises per kg the following ingredients: 3.2g of vitamin A; vitamin D3, 0.5 g; vitamin E, 24 g; vitamin K3, 1 g; vitamin B1, 2.5 g; riboflavin (80%), 4.5 g; pyridoxine, 2 g; vitamin B12 (1%), 1 g; vitamin C (35%), 200 g; calcium pantothenate, 6g; nicotinic acid, 20 g; inositol, 80 g; biotin (2%), 6 g; folic acid, 2 g; choline chloride (50%), 250 g; FeSO4·H2O，8g；ZnSO4·H2O，5g；CuSO4·5H2O，1g；MnSO4·H2O，4.5g；KI，6g；CoCl2·6H2O(1％)，5g；Na2SeO3(1％)，2g；MgSO4·7H2O, 120 g; NaCl, 100 g; 100g of calcium propionate and 45.8g of cellulose.
Example 1
A feed additive for preventing and treating bovine frog ascites comprises the following components in parts by weight: 15 parts of fructus forsythiae, 30 parts of radix bupleuri, 2 parts of allicin, 2 parts of bile acid, 15 parts of lactobacillus acidophilus freeze-dried powder, 25 parts of bacillus subtilis freeze-dried powder and 11 parts of corn starch.
The using method comprises the following steps: 1 part of the prepared additive and 99 parts of common bullfrog compound feed are uniformly mixed, then are puffed and granulated, and are packaged for later use after being dried, cooled and screened.
Example 2
A feed additive for preventing and treating bovine frog ascites comprises the following components in parts by weight: 30 parts of fructus forsythiae, 15 parts of radix bupleuri, 1 part of allicin, 4 parts of bile acid, 25 parts of lactobacillus acidophilus freeze-dried powder, 15 parts of bacillus subtilis freeze-dried powder and 10 parts of corn starch.
The using method comprises the following steps: 1 part of the prepared additive and 99 parts of common bullfrog compound feed are uniformly mixed, then are puffed and granulated, and are packaged for later use after being dried, cooled and screened.
Example 3
A feed additive for preventing and treating bovine frog ascites comprises the following components in parts by weight: 20 parts of fructus forsythiae, 20 parts of radix bupleuri, 1 part of allicin, 2 parts of bile acid, 20 parts of lactobacillus acidophilus freeze-dried powder, 20 parts of bacillus subtilis freeze-dried powder and 17 parts of corn starch.
The using method comprises the following steps: 1 part of the prepared additive and 99 parts of common bullfrog compound feed are uniformly mixed, then are puffed and granulated, and are packaged for later use after being dried, cooled and screened.
Comparative test example 1
The breeding test is carried out on a certain breeding farm in south pond town of Shanfeng city, Lufeng city, Shanshan province, Guangdong province from 2019, 6 and 20 days to 2019, 10 and 18 days. Selecting 16 thousands of bullfrogs with healthy appearance and uniform specification, respectively transferring the bullfrogs into 16 test pools (15m multiplied by 4m multiplied by 1.0m, the water depth: 0.8m), and temporarily culturing 10000 bullfrogs in each pool for one week. During the temporary rearing period, the common bullfrog compound feed is used for feeding for 2 times (7:00, 17:00) every day, so that the feed is suitable for the rearing environment and feed. Then, the feed was randomly divided into 4 groups, and the feed was fed to comparative example 1 (normal bullfrog mixed feed), example 1, example 2 and example 3, 4 times per group, and manually fed at a timing of 7:00 and 17:00 per day, so that the bullfrogs were apparently satiated. The daily food intake, number of dead frogs in ascites disease and weight of dead frogs were recorded. The test period was 120 days, and the frog in each pond was weighed at the end of the test, and the test results are shown in table 1.
Table 1: comparative test example 1 Bullfrog mortality and growth Performance data
Example 1 Example 2 Example 3 Comparative example 1
Initial average amount (g) 23.5±0.3 23.1±0.2 23.2±0.2 23.7±0.4
Final average dose (g) 249.1±3.7a 256.1±4.5a 253.2±3.6a 224.6±2.8b
Weight gain (%) 960.0±14.3a 1008.7±17.7a 991.4±14.1a 847.7±10.6b
Feed factor (weight of dead frog in calculation) 0.93±0.01a 0.90±0.02a 0.92±0.02a 1.02±0.01b
Cost of frog feed (Yuan/jin frog) 3.82±0.04ab 3.70±0.06a 3.78±0.08ab 3.92±0.03b
Total mortality (%) 1.7±0.2a 1.3±0.2a 1.5±0.1a 4.6±0.6b
Ascites disease mortality (%) 0.8±0.0a 0.6±0.1a 0.7±0.1a 3.3±0.5b
Note: 1. the difference is significant (P <0.05) for different lower case letters after the same row of data in the table; 2. the feed prices of examples 1 to 3 were calculated to 8200 yuan/ton and the feed price of comparative example 1 was calculated to 7700 yuan/ton.
As can be seen from Table 1, the final body weight and the weight gain rate of the samples 1 to 3 are all significantly higher than those of the sample 1, and the feed coefficient, the total death rate and the ascites disease death rate are all significantly lower than those of the sample 1; the above indexes are not significantly different from example 1 to example 3. The cost of the rana grahami feed in example 2 is significantly lower than that in comparative example 1, but example 1 and example 3 have no significant difference from comparative example 1, and no significant difference exists between example 1 and example 3.
The results of the comparative experiment show that the feed additive can obviously reduce the total death rate, the ascites death rate and the feed coefficient of the bullfrogs, and simultaneously obviously improve the growth speed of the bullfrogs, and has obvious overall economic benefit.
Comparative test example 2
The breeding contrast test is carried out on a certain farm in Chenghai district, Yanhong town of Shantou city, Guangdong province from 8 months 6 days in 2019 to 8 months 20 days in 2019. The farm has 6 culture ponds with the same specification (20m multiplied by 6m multiplied by 1m, the water depth is 0.8m, the tail of each pond is placed with a frog 18000 without loss before the pond), a small amount of bullfrogs in each pond are found to have abdominal dropsy at the bottom of 7 months, then the water change amount is increased every day and the whole pond is disinfected, the improvement is still not good by 5 days after 8 months, and the morbidity and the mortality are continuously increased. The comparative treatment protocol was started at 6.8 months and 6 ponds were randomly divided into 2 groups of 3 replicates each, the first group was fed with norfloxacin mix (comparative example 2) at 2g/kg feed and the second group was fed with the feed of example 2, and the bullfrogs were apparently satiated by manual feeding at 7:00 and 17:00 times per day. And (4) the comparison period is 15 days, the death number of each pool is recorded every day, water samples of each pool are collected at the beginning, the end and the 7 th day of the comparison, and the number of aeromonas hydrophila in the water body is detected. The test results are shown in FIG. 1 and Table 2.
Table 2: comparative test example 2 the number of Aeromonas hydrophila in Water
Figure BDA0002861007300000061
Note: the difference is significant for different lower case letters after the same row data in the table (P <0.05)
As can be seen from fig. 1, the average daily mortality in example 1 was significantly lower than that in comparative example 1, and death had ceased at day 8-19, whereas the mortality in comparative example 1 did not change significantly after day 8-14 and continued to be high.
As can be seen from Table 2, the number of Aeromonas hydrophila in the water body on the 7 th day of the comparison and at the end of the comparison in the comparison period of example 2 was also significantly lower than that in comparative example 2.
The results of the test show that the additive can effectively prevent and treat the bovine frog ascites disease by adding into the feed, and the effect is obviously better than that of norfloxacin.
Comparative test example 3
The breeding contrast test is carried out on a certain breeding farm in the south town of the Chenghai district xi of Shantou city of Guangdong province from 9 months 9 to 19 months 9 and 2019 in 2019. The farm has 10 culture ponds with the same specification (15m multiplied by 6m multiplied by 1m, the water depth is 0.8m, 10000 frogs are placed in each pond without loss before the pond), a small amount of frogs in each pond are found to have ascites disease in the early 9 months, then the water change amount is increased every day and the whole pond is disinfected, the disease rate and the death rate are continuously increased after 9 months and 8 days. The comparative treatment scheme was started on 9 months and 9 days, 10 breeding ponds were randomly divided into 2 groups, each group was repeated 5 times, the first group was fed with the compound sulfamethoxazole mix (comparative example 3) at a dose of 3g/kg feed, and the second group was fed with the feed of example 3 at regular times of 7:00 and 17:00 per day to apparently satiate bullfrogs. And (3) the comparison period is 11 days, the death number of each pool is recorded every day, water samples of each pool are collected at the beginning, the end and the 7 th day of the comparison, and the number of aeromonas hydrophila in the water body is detected. The test results are shown in fig. 2 and table 3.
Table 3: comparative test example 3 the number of Aeromonas hydrophila in Water
Figure BDA0002861007300000062
Note: the difference is significant for different lower case letters after the same row data in the table (P <0.05)
As can be seen from fig. 2, the average daily mortality in example 3 was significantly lower than that of comparative example 3, and death had ceased at 19 days 9 months, whereas the mortality in comparative example 3 did not change significantly after 15 days 9 months and continued to stay high.
As can be seen from Table 3, the number of Aeromonas hydrophila in the water body on the 7 th day of the comparison and at the end of the comparison in example 3 was also significantly lower than in comparative example 3 during the comparison period.
The results of the test show that the additive can effectively prevent and treat the bovine frog ascites disease by adding into the feed, and the effect is obviously better than that of the compound sulfamethoxazole.
Comparative test example 4
To show the synergistic effect of the components in the additive of the present invention, 6 additives lacking a single component were prepared on the basis of example 1. The 1 st lacks fructus forsythiae, the 2 nd lacks radix bupleuri, the 3 rd lacks bile acid, the 4 th lacks allicin, the 5 th lacks lactobacillus acidophilus freeze-dried powder and the 6 th lacks bacillus subtilis freeze-dried powder. The 6 single component missing additive formulations are shown in table 4.
Table 4: example 1 and deficiency formulations
Figure BDA0002861007300000071
The breeding test is carried out on a certain breeding farm in south pond town of Shanfeng city, Lufeng city, Shanshan province, Guangdong province from 2019, 6 and 20 days to 2019, 10 and 18 days. Selecting 28 thousands of bullfrogs with healthy appearance and uniform specification, respectively transferring the bullfrogs into 28 test ponds (15m multiplied by 4m multiplied by 1.0m, the water depth: 0.8m), and temporarily culturing 10000 bullfrogs in each pond for one week. During the temporary rearing period, the common bullfrog compound feed is used for feeding for 2 times (7:00, 17:00) every day, so that the feed is suitable for the rearing environment and feed. Then, the bullfrog is randomly divided into 7 groups, each group is repeated for 4 times, the feeds of the embodiment 1 and the deletion examples 1-6 are respectively fed, and the bullfrog is artificially fed at the fixed time of 7:00 and 17:00 every day to ensure that the bullfrog is apparently satiated. The daily food intake, number of dead frogs in ascites disease and weight of dead frogs were recorded. The test period was 120 days, and the frog in each pond was weighed at the end of the test, and the test results are shown in table 5.
Table 5: comparative experiment 4 for bullfrog mortality and growth performance data
Figure BDA0002861007300000072
Figure BDA0002861007300000081
As can be seen from table 5, the terminal body weight, the weight gain rate and the feed factor of example 1 are not significantly different from those of deletion examples 1 and 2, but the terminal body weight and the weight gain rate of example 1 are significantly higher than those of deletion examples 3 to 6, and the feed factor is significantly lower than those of deletion examples 3 to 6; the total mortality rate and ascites disease mortality rate of example 1 were significantly lower than those of deletion examples 1-6.
The results of the comparative experiments show that the feed additive can obviously reduce the total death rate, the ascites death rate and the feed coefficient of bullfrogs, and simultaneously obviously improve the growth speed of the bullfrogs, and the components of the feed additive have synergistic action.
In addition, the inventor further researches the effect of the weight ratio of the components in the feed additive disclosed by the invention by using similar experiments, and proves that the mortality rate, the ascites disease mortality rate and other effects are better by using the weight ratio disclosed by the invention, so that the effect of the feed additive is remarkably improved by using the weight ratio disclosed by the invention.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and their concepts should be considered to be equivalent or modified within the technical scope of the present invention.

Claims (10)

1. The feed additive for preventing and treating the bovine frog ascites disease is characterized by comprising the following components in parts by weight: 15-30 parts of fructus forsythiae, 15-30 parts of radix bupleuri, 1-2 parts of allicin, 2-4 parts of bile acid, 15-25 parts of lactobacillus acidophilus freeze-dried powder, 15-25 parts of bacillus subtilis freeze-dried powder and 10-20 parts of corn starch.
2. The feed additive for preventing and treating bovine frog ascites disease according to claim 1, which is characterized by comprising the following components in parts by weight: 15 parts of fructus forsythiae, 30 parts of radix bupleuri, 2 parts of allicin, 2 parts of bile acid, 15 parts of lactobacillus acidophilus freeze-dried powder, 25 parts of bacillus subtilis freeze-dried powder and 11 parts of corn starch.
3. The feed additive for preventing and treating bovine frog ascites disease according to claim 1, which is characterized by comprising the following components in parts by weight: 30 parts of fructus forsythiae, 15 parts of radix bupleuri, 1 part of allicin, 4 parts of bile acid, 25 parts of lactobacillus acidophilus freeze-dried powder, 15 parts of bacillus subtilis freeze-dried powder and 10 parts of corn starch.
4. The feed additive for preventing and treating bovine frog ascites disease according to claim 1, which is characterized by comprising the following components in parts by weight: 20 parts of fructus forsythiae, 20 parts of radix bupleuri, 1 part of allicin, 2 parts of bile acid, 20 parts of lactobacillus acidophilus freeze-dried powder, 20 parts of bacillus subtilis freeze-dried powder and 17 parts of corn starch.
5. The feed additive for preventing and treating bovine frog ascites as claimed in any one of claims 1 to 4, wherein the bile acid comprises 20% by weight of hyocholic acid, 60% by weight of hyodeoxycholic acid, and 20% by weight of chenodeoxycholic acid; and/or the viable count of the lactobacillus acidophilus freeze-dried powder after recovery is not less than 1 multiplied by 109cfu/g; and/or the number of viable bacteria after the bacillus subtilis freeze-dried powder recovers is not less than 1 multiplied by 109cfu/g。
6. A method for preparing the feed additive for preventing and treating bovine frog ascites disease of any one of claims 1 to 5, comprising the following steps:
(1) pulverizing fructus forsythiae and bupleuri radix respectively, and sieving;
(2) mixing sieved fructus forsythiae and bupleuri radix with garlicin, bile acid, Lactobacillus acidophilus lyophilized powder, Bacillus subtilis lyophilized powder and corn starch, and stirring to obtain mixture;
(3) and (3) performing secondary crushing on the mixture obtained in the step (2), sieving, and uniformly stirring the sieved mixture to obtain the feed additive.
7. The method of claim 6, comprising the steps of:
(1) pulverizing fructus forsythiae and bupleuri radix respectively, and sieving with 40 mesh sieve;
(2) mixing fructus forsythiae and bupleuri radix sieved by a 40-mesh sieve with garlicin, bile acid, lactobacillus acidophilus lyophilized powder, bacillus subtilis lyophilized powder and corn starch, and stirring uniformly to obtain a mixture;
(3) and (3) performing secondary crushing on the mixture obtained in the step (2), sieving by using a 100-mesh sieve, and uniformly stirring the sieved mixture to obtain the feed additive.
8. A feed additive prepared by the preparation method according to claim 6 or 7.
9. The application of the feed additive for preventing and treating the bullfrog ascites is characterized in that the additive is the additive disclosed in any one of claims 1-5 and 6-7, and the additive is added into a bullfrog compound feed in an amount of 0.5-2% in percentage by weight; preferably, the additive is added into the bullfrog compound feed in a weight percentage of 1%.
10. Use of a feed additive for the prevention and treatment of bullfrog ascites disease, characterised in that the additive is a feed additive according to any one of claims 1 to 5 and 6 to 7 for the prevention and/or treatment of bullfrog ascites disease and/or for increasing the rate of weight gain and/or for reducing the feed factor and/or for reducing the mortality rate, including mortality due to ascites disease.
CN202011563424.0A 2020-12-25 2020-12-25 Feed additive for preventing and treating bovine frog ascites disease and preparation method and application thereof Pending CN112655816A (en)

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