CN105165310A - Cultivation method for improving output of ligusticum wallichii - Google Patents
Cultivation method for improving output of ligusticum wallichii Download PDFInfo
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- CN105165310A CN105165310A CN201510427678.2A CN201510427678A CN105165310A CN 105165310 A CN105165310 A CN 105165310A CN 201510427678 A CN201510427678 A CN 201510427678A CN 105165310 A CN105165310 A CN 105165310A
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- ligusticum wallichii
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- quicklime
- siberian cocklebur
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Abstract
The invention discloses a cultivation method for improving the output of ligusticum wallichii. The method comprises the following steps: a, soil treatment: uniformly mixing quicklime with surface soil, wherein the usage amount of quicklime is 50-200 kg/mu; and b, cultivating ligusticum wallichii seeds. The method disclosed by the invention can be used for effectively improving the output of ligusticum wallichii (the improved range is 19.04-66.08%) and is good in application prospect.
Description
Technical field
The present invention relates to a kind of method improving Ligusticum wallichii output.
Background technology
Ligusticum wallichii begins to be loaded in Shennong's Herbal, name rhizome of chuanxiong Qiong, " the pungent temperature of taste.Main apoplexy enters brain, headache, arthritis, muscle contraction, emergency, and gold wound, married woman's blood closes, s.m.p.Raw Chuan Gu." " Wu Pu Bencao " growing environment of Ligusticum wallichii, medicinal plants form and harvest time done described in more detail " or raw Hu Wutao ubac, or Taishan, leaf is fragrant thin blue or green black, Wen Chiru Jehol Ligusticum Rhizome, summer in winter grows thickly, and May, China was red, and July is real black, stem end two leaf, March adopts, and root has joint, like gag bit shape." carried out supplementing " saying: a Hu to the processing method of Ligusticum wallichii in " Mingyi Bielu "
, a fragrant fruit, its leaf name seedling of xiongqiong, raw acrobatic skill diagonal valley Xi Ling, in April in March, adopts root suddenly and violently dry." cultivation history of rhizome of chuanxiong Qiong, can review here beam period, " Tang materia medica " is said: " shape block is large for rhizome of chuanxiong Qiong, Jin Chu Qin Zhou, its human world kind person, and heavy real, greasiness moistens, and in mountain, the person of adopting is carefully thin." with Ligusticum wallichii be rectification of name the earliest when " medicine origin " that pushes away Zhang Yuansu.Although ancient times, various places had rhizome of chuanxiong Qiong medicinal material to produce, its quality generally acknowledges with Shu Chuan to be victory from the Song dynasty, the dry rhizome of its former plant and present Ligusticum wallichii LigusticumchuanxiongHort..
Ligusticum wallichii happiness is had a moderate climate, rainfall is abundant, sunshine is sufficient and more moistening, the environment of the subtropics monsoon climate that day and night temperature is large.Deep at soil layer, loose fertile, draining is good, the content of organic matter is abundant, well-grown in neutrality or slightly acidic sand loam.Ligusticum wallichii is planted in the beginning of autumn of around in early August usually, gathers about the first tenday period of a month May next year, 280 ~ 290 days vegetative period.
In recent years the demand of market to Ligusticum chuanxiong Hort is increase tendency, but, because Ligusticum chuanxiong Hort market price fluctuations causes greatly the enthusiasm of Cotton Varieties by Small Farming Households Ligusticum wallichii to decline, and then cause Ligusticum wallichii output can not meet the demand in market, be badly in need of wanting finding method to improve the output of Ligusticum wallichii.
Summary of the invention
In order to solve the problem that existing Ligusticum wallichii yields poorly, the invention provides a kind of method improving Ligusticum wallichii output.
The present invention improves the cultivation method of Ligusticum wallichii output, comprises the following steps:
A, soil treatment: preparing quick lime, mix with table soil, the usage amount of quicklime is 50 ~ 200kg/ mu;
B, get Ligusticum wallichii Siberian cocklebur kind, cultivation, results.
Table soil, refer to the top of earth, thickness is 5 ~ 10cm.
Preferably, in step a, the usage amount of quicklime is 50kg/ mu or 150kg/ mu.
Preferably, in step b, the step of described cultivation is as follows:
(1) Ligusticum wallichii Siberian cocklebur kind is got, sowing;
(2) fill the gaps with seedlings, intertill and clean tillage, fertilising;
(3) gather, dry.
Preferably, in step (1), the time of described sowing is mid or late August.
Preferably, in step (1), the amount of described Ligusticum wallichii Siberian cocklebur kind sowing is every mu of sowing 30 ~ 40kg.
Preferably, in step (2), the mode of fertilising is: rise from mid-September, uses 1 excrement clear water every 15 ~ 20 days, and totally 3 times, amount of application is respectively: 1000kg/ mu, 1000kg/ mu, 2000kg/ mu; After turning green in 2 ~ March of next year, then enrich 1 excrement clear water, consumption is 1000kg/ mu.
Excrement clear water: for farmyard manure becomes thoroughly decomposed the liquid fertilizer of retting completely.Farmyard manure: the fecaluria of people, the fecaluria of livestock breeding and barnyard manure, green manure etc.
Turn green, refer to plant seedling replanting or after surviving the winter, become green from yellow, and restoration ecosystem.
Preferably, in step b, the region of cultivation is Ao Ping town, Pengzhou City, Sichuan Province, and its longitude is east longitude 103 ° 59 ' 16.53 ", latitude is north latitude 31 ° 0.4 ' 52.33 ".
The inventive method can effectively improve Ligusticum wallichii output, and increase rate is 19.04% ~ 66.08%, and application prospect is good.
The embodiment of form by the following examples, is described in further detail foregoing of the present invention.But this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following embodiment.All technology realized based on foregoing of the present invention all belong to scope of the present invention.
Accompanying drawing explanation
The chromatogram of Fig. 1 forulic acid reference substance
The chromatogram of Fig. 2 Ligusticum wallichii test sample
Embodiment
Embodiment 1 Ligusticum wallichii cultivation method of the present invention
1 material
Ligusticum wallichii Siberian cocklebur kind derives from small fish hole town, Pengzhou City, Sichuan Province.Quicklime is purchased from Emeishan City nine Li Xingmin pulverized limestone factory.Excrement clear water is from local farmers'.
2. region
Ao Ping town, Pengzhou City, Sichuan Province, longitude is east longitude 103 ° 59 ' 16.53 ", latitude is north latitude 31 ° 0.4 ' 52.33 ".
3 methods
3.1 experiment groupings
Be divided into 5 groups, each group of area under cultivation is plot area a: 10m
2, often group establish 3 times parallel.Before Ligusticum wallichii sowing, quicklime and table soil (are shown soil and referred to the top of earth, thickness is 5 ~ 10cm) Homogeneous phase mixing, wherein the addition of quicklime is respectively 50kg/ mu, 100kg/ mu, 150kg/ mu, 200kg/ mu, not add the soil of quicklime for control group.
3.2 cultivation method
3.2.1 field seeding
3.2.1.1 carefully reject again before Siberian cocklebur (Siberian cocklebur i.e. Siberian cocklebur kind) process sowing and have upper Siberian cocklebur sprouted without bud or bud of worm channel, joint dish hollow and joint, directly plant down.
3.2.1.2 mid or late August in sowing time.
3.2.1.3 before sowing, quicklime mixes with table soil by land treatment systems.
3.2.1.4 1.5m spacing railway carriage or compartment is pressed in type of seeding plot, by seeding row spacing 25cm × 30cm, is upwards buried by have gentle hands light press by Siberian cocklebur bud head, touches soil and is advisable, expose half and show at soil to save dish.After cultivation, with the joint dish of rice straw mulching Siberian cocklebur, and water excrement clear water (1000kg/ mu).Every mu with Siberian cocklebur 30 ~ 40kg.
3.2.2 field management
3.2.2.1 fill the gaps with seedlings for being short of seedling, bad seedling, can the cloudy day be selected, take Siberian cocklebur for subsequent use and fill the gaps with seedlings, balled transplanting, must water after benefit.The work of filling the gaps with seedlings must terminate before the Autumnal Equinox.
3.2.2.2 after intertill and clean tillage early September neat seedling, shallow hoe intertillage 1 time and will Ligusticum wallichii in the ranks mulching straw (shallow hoe intertillage refers to the surface tillage adopting the instruments such as hand hoe to carry out between Ligusticum wallichii plant, have loosen the soil, weeding, the effect that heats, preserve soil moisture).In 1 month next year, the last ten-days period, when ground cauline leaf starts the withered and yellow time of falling seedling, withering cauline leaf in cleaning field, and earths up, and producing region claims " ulling up winter medicine ".
3.2.2.3 rich water quality management controls to rise from mid-September with growth, and used 1 excrement clear water every 15 ~ 20 days and carry seedling, totally 3 times, amount of application is respectively: 1000kg/ mu, 1000kg/ mu, 2000kg/ mu; After turning green in 2 ~ March of next year, then enrich 1 excrement clear water, consumption is 1000kg/ mu.
Early April pinches to the acrial part pinching that growing way is prosperous.
3.2.3 pick and process
3.2.3.1 late April of Second Year after cultivation of gathering gathers.Select fine day, dig out rhizome, shake off earth, removing cauline leaf.Slightly to dry in the air in field aqueous vapor, transport drying back.
3.2.3.2 Habitat producing dries.
3.3 detect
1 increment product are adopted in each community, and every increment product get 30 strains at random.Unification selects impurity elimination after gathering, section, dries, and pulverizes, and crosses 60 mesh sieves, makes medicinal powder, carried out mensuration and the analysis of biological yield.
Data acquisition MicrosoftExce12013 and SPSS13 carries out adding up and Treatment Analysis.
Ethanol soluble extractives assay measures according to method under " Chinese Pharmacopoeia " 2010 editions Ligusticum wallichii items.
Volatile oil content testing extracts with reference to " Chinese Pharmacopoeia " 2010 editions one annex XD second method steam distillation and measures.
Ferulaic acid content measures and measures with reference to method under " Chinese Pharmacopoeia " 2010 editions Ligusticum wallichii items.
Chromatographic condition chromatographic column is KromasilC18 (5 μm, 250mm × 4.6mm): mobile phase is methyl alcohol-1% acetum (30:70); Determined wavelength 321nm; Flow velocity 1.0mlmin
-1, column temperature 30 DEG C, sample size 10 μ l.The chromatogram of reference substance solution, need testing solution is shown in Fig. 1 ~ 2.
The preparation precision of reference substance solution takes forulic acid reference substance 100 μ g, puts in the brown volumetric flask of 5ml, adds 70% methyl alcohol and dissolve and be settled to scale, shake up, and obtaining concentration is 20.0 μ gml
-1reference substance solution.
Each sample medicinal powder (crossing No. four sieves) 0.5g is got in the preparation of sample solution, accurately weighed, puts in tool plug conical flask, precision adds 70% methyl alcohol 50ml, weighed weight after close plug, heating water bath backflow 30min, let cool, more weighed weight, the weight of less loss is supplied with 70% methyl alcohol, shake up, leave standstill, get supernatant, miillpore filter (0.45 μm) filters, get subsequent filtrate, to obtain final product.
The drafting precision of calibration curve draws reference substance solution 2,5,10,15,20 μ l sample introduction, and with forulic acid integrating peak areas value for ordinate, its content is abscissa, drawing standard curve.Regression equation is y=27.8848648x-1.1489417, r=0.9999, and ferulaic acid content is good linear relation with integrating peak areas value within the scope of 0.04 ~ 0.50 μ g.
Sample size measures accurate pipette samples solution 10 μ l sample introduction, by above-mentioned chromatographic condition working sample content.
The chromatogram of forulic acid reference substance and the chromatogram of Ligusticum wallichii test sample are as Fig. 1 ~ Fig. 2.
4 testing results
Through detecting, the Ligusticum wallichii output of each group is as shown in the table:
Table 1 quicklime process experimental result (
n=3)
Note: not alphabetical representative exists significant difference between processing under 0.05 level, and it is not remarkable that same letter then represents difference between process.
The results show, adopt the present invention to add the mode of the quicklime of 50 ~ 200kg/ mu, can significantly improve the output of Ligusticum wallichii, increase rate is 19.04% ~ 66.08%; Within the scope of addition of the present invention, Ligusticum wallichii output is along with the increase of quicklime, after first reducing, rising reduces again, wherein, the addition of quicklime be 50kg/ mu or 150kg/ mu time, output increased is the most obvious, and increase rate is respectively 66.08% and 62.15%, in conjunction with the active constituent content of Ligusticum wallichii, most preferred quicklime addition is 150kg/ mu.
Comparative example
1 material
With embodiment 1.
2. region
With embodiment 1.
3 cultivation methods
Addition except quicklime is except 250kg/ mu, and all the other steps are identical with embodiment 1.
4 testing results
Through detecting, when the addition of quicklime is 250kg/ mu, the output of Ligusticum wallichii is 193.29kg667m
-2, and control group is 206.67kg667m
-2, when the addition that quicklime is described is 250kg/ mu, the output of Ligusticum wallichii can be reduced on the contrary.
To sum up, the present invention, by adding the quicklime of specified quantitative, can significantly improve the output of Ligusticum wallichii, wherein, the addition of quicklime be 50kg/ mu or 150kg/ mu time, output increased is the most obvious, and the quicklime added outside particular range of the present invention, then cannot improve output, can output be reduced on the contrary.
Claims (7)
1. improve a cultivation method for Ligusticum wallichii output, it is characterized in that: comprise the following steps:
A, soil treatment: preparing quick lime, mix with table soil, the usage amount of quicklime is 50 ~ 200kg/ mu;
B, get Ligusticum wallichii Siberian cocklebur kind, cultivation, results.
2. cultivation method according to claim 1, is characterized in that: in step a, and the usage amount of quicklime is 50kg/ mu or 150kg/ mu.
3. cultivation method according to claim 1, is characterized in that: in step b, and the step of described cultivation is as follows:
(1) Ligusticum wallichii Siberian cocklebur kind is got, sowing;
(2) fill the gaps with seedlings, intertill and clean tillage, fertilising;
(3) gather, dry.
4. cultivation method according to claim 3, is characterized in that: in step (1), and the time of described sowing is mid or late August.
5. cultivation method according to claim 3, is characterized in that: in step (1), and the seeding quantity of described Ligusticum wallichii Siberian cocklebur kind is every mu of 30 ~ 40kg.
6. cultivation method according to claim 3, it is characterized in that: in step (2), the mode of fertilising is: rise from mid-September, used 1 excrement clear water every 15 ~ 20 days, totally 3 times, amount of application is respectively: 1000kg/ mu, 1000kg/ mu, 2000kg/ mu; After turning green in 2 ~ March of next year, then enrich 1 excrement clear water, consumption is 1000kg/ mu.
7. cultivation method according to claim 1, is characterized in that: in step b, and the region of cultivation is Ao Ping town, Pengzhou City, Sichuan Province, and its longitude is east longitude 103 ° 59 ' 16.53 ", latitude is north latitude 31 ° 0.4 ' 52.33 ".
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CN101322974A (en) * | 2008-07-04 | 2008-12-17 | 安徽省农业科学院土壤肥料研究所 | Soil heavy metal passivation agent for vegetable field |
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CN101322974A (en) * | 2008-07-04 | 2008-12-17 | 安徽省农业科学院土壤肥料研究所 | Soil heavy metal passivation agent for vegetable field |
CN102220136A (en) * | 2011-03-30 | 2011-10-19 | 中国科学院亚热带农业生态研究所 | Soil heavy metal composite passivator with humic acid minerals and preparation method thereof |
CN104478058A (en) * | 2014-11-04 | 2015-04-01 | 沈礼群 | Pond-lake heavy-metal pesticide fertilizer pollutant solidifying removing agent and preparation method thereof |
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