CN104938205A - Cultivation method capable of reducing cadmium content in ligusticum wallichii - Google Patents
Cultivation method capable of reducing cadmium content in ligusticum wallichii Download PDFInfo
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- CN104938205A CN104938205A CN201510428524.5A CN201510428524A CN104938205A CN 104938205 A CN104938205 A CN 104938205A CN 201510428524 A CN201510428524 A CN 201510428524A CN 104938205 A CN104938205 A CN 104938205A
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- ligusticum wallichii
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a cultivation method capable of reducing cadmium content in ligusticum wallichii. The cultivation method comprises the following steps: a, soil treatment: taking caustic lime to be uniformly mixed with surface soil, wherein the usage amount of caustic lime is 200 kg per mu; b, taking ligusticum wallichii stems, and then cultivating. The cultivation method capable of reducing cadmium content in ligusticum wallichii, provided by the invention, can effectively reduce the cadmium content in ligusticum wallichii, and at the same time can effectively increase the contents of extract of effective constituents and ferulic acid of the ligusticum wallichii and has a good application prospect.
Description
Technical field
The present invention relates to a kind of cultivation method reducing Ligusticum wallichii cadmium content.
Background technology
Ligusticum wallichii is the dry rhizome of samphire Ligusticum wallichii Ligusticum chuanxiong Hort..For chest impediment and cardialgia, the shouting pain of the chest side of body, tumbling and swelling, irregular menstruation, through closing dysmenorrhoea ， Disorder lump in the abdomen stomachache, headache, arthralgia due to wind-dampness.Modern pharmacodynamic study proves that Ligusticum wallichii mainly has affects female reproductive system, cardiovascular system, nervous system, respiratory system, digestive system, analgesia, anti-inflammatory, antipyretic, effect such as antitumor grade.Forulic acid is also one of main active of Ligusticum wallichii, has platelet aggregation-against, suppresses platelet 5-HT release, suppresses the generation of platelet thrombus element a2 (txa2), strengthen prostaglandin activity, analgesia, the effects such as alleviating vascular spasm.Therefore, the content improving the active component forulic acid of Ligusticum wallichii when cultivating is significant.
In addition, along with people more and more pay close attention to food and pharmaceutical safety, test stone for food and medicine also becomes all the more harsh, and the bulk medicinal materials outlet of Ligusticum wallichii all the time as China's Chinese herbal medicine class is American-European, but because its heavy metal cadmium content exceeds standard since the nineties in 20th century, the drug safety that have impact on Ligusticum wallichii is kept outside of the door by multiple country, has also had a strong impact on its foreign exchange earning.Therefore, the cultivation method of research reduction Ligusticum wallichii cadmium content is very urgent.
The happiness of Ligusticum wallichii plant is had a moderate climate, rainfall is abundant, sunshine is sufficient and more moistening, the environment of the subtropics monsoon climate that day and night temperature is large.Deep at soil layer, loose fertile, draining is good, the content of organic matter is abundant, well-grown in neutrality or slightly acidic sand loam.Ligusticum wallichii is planted in the beginning of autumn of around in early August usually, gathers about the first tenday period of a month May next year, 280 ~ 290 days vegetative period.
Summary of the invention
In order to solve the high problem of existing Ligusticum wallichii cadmium content, the invention provides a kind of method reducing Ligusticum wallichii cadmium content.
The present invention reduces the cultivation method of Ligusticum wallichii cadmium content, comprises the following steps:
A, soil treatment: preparing quick lime, mix with table soil, the usage amount of quicklime is 200kg/ mu;
B, get Ligusticum wallichii Siberian cocklebur kind, cultivation.
Table soil, refer to the top of earth, thickness is 5 ~ 10cm.
Preferably, in step b, the step of described cultivation is as follows:
(1) Ligusticum wallichii Siberian cocklebur kind is got, sowing;
(2) fill the gaps with seedlings, intertill and clean tillage, fertilising;
(3) gather, dry.
Preferably, in step (1), the time of described sowing is mid or late August.
Preferably, in step (1), the amount of described Ligusticum wallichii Siberian cocklebur kind sowing is every mu of sowing 30 ~ 40kg.
Preferably, in step (2), the mode of fertilising is: rise from mid-September, uses 1 excrement clear water every 15 ~ 20 days, and totally 3 times, amount of application is respectively: 1000kg/ mu, 1000kg/ mu, 2000kg/ mu; After turning green in 2 ~ March of next year, then enrich 1 excrement clear water, consumption is 1000kg/ mu.
Excrement clear water: for farmyard manure becomes thoroughly decomposed the liquid fertilizer of retting completely.Farmyard manure: the fecaluria of people, the fecaluria of livestock breeding and barnyard manure, green manure etc.
Turn green, refer to plant seedling replanting or after surviving the winter, become green from yellow, and restoration ecosystem.
Preferably, in step b, the region of cultivation is Ao Ping town, Pengzhou City, Sichuan Province, and its longitude is east longitude 103 ° 59 ' 16.53 ", latitude is north latitude 31 ° 0.4 ' 52.33 ".
The inventive method significantly can reduce the cadmium content of Ligusticum wallichii, and effectively can improve the active ingredient extract of Ligusticum wallichii and the content of forulic acid, application prospect is good simultaneously.
The embodiment of form by the following examples, is described in further detail foregoing of the present invention.But this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following embodiment.All technology realized based on foregoing of the present invention all belong to scope of the present invention.
Accompanying drawing explanation
The chromatogram of Fig. 1 forulic acid reference substance
The chromatogram of Fig. 2 Ligusticum wallichii test sample
Embodiment
Embodiment 1 Ligusticum wallichii cultivation method of the present invention
1 material
Ligusticum wallichii Siberian cocklebur kind derives from small fish hole town, Pengzhou City, Sichuan Province.Quicklime is purchased from Emeishan City nine Li Xingmin pulverized limestone factory.Excrement clear water is from local farmers'.
2. region
Ao Ping town, Pengzhou City, Sichuan Province, longitude is east longitude 103 ° 59 ' 16.53 ", latitude is north latitude 31 ° 0.4 ' 52.33 ".
3 methods
3.1 experiment groupings
Be divided into 2 groups, each group of area under cultivation is plot area a: 10m
2, often group establish 3 times parallel.By quicklime and table soil (show soil and refer to the top of earth, thickness is 5 ~ 10cm) Homogeneous phase mixing before Ligusticum wallichii sowing, wherein the addition of quicklime is respectively 200kg/ mu, not add the soil of quicklime for control group.
3.2 cultivation method
3.2.1 field seeding
3.2.1.1 carefully reject again before Siberian cocklebur (Siberian cocklebur i.e. Siberian cocklebur kind) process sowing and have upper Siberian cocklebur sprouted without bud or bud of worm channel, joint dish hollow and joint, directly plant down.
3.2.1.2 mid or late August in sowing time.
3.2.1.3 before sowing, quicklime mixes with table soil by land treatment systems.
3.2.1.4 1.5m spacing railway carriage or compartment is pressed in type of seeding plot, by seeding row spacing 25cm × 30cm, is upwards buried by have gentle hands light press by Siberian cocklebur bud head, touches soil and is advisable, expose half and show at soil to save dish.After cultivation, with the joint dish of rice straw mulching Siberian cocklebur, and water excrement clear water (1000kg/ mu).Every mu with Siberian cocklebur 30 ~ 40kg.
3.2.2 field management
3.2.2.1 fill the gaps with seedlings for being short of seedling, bad seedling, can the cloudy day be selected, take Siberian cocklebur for subsequent use and fill the gaps with seedlings, balled transplanting, must water after benefit.The work of filling the gaps with seedlings must terminate before the Autumnal Equinox.
3.2.2.2 after intertill and clean tillage early September neat seedling, shallow hoe intertillage 1 time and will Ligusticum wallichii in the ranks mulching straw (shallow hoe intertillage refers to the surface tillage adopting the instruments such as hand hoe to carry out between Ligusticum wallichii plant, have loosen the soil, weeding, the effect that heats, preserve soil moisture).In 1 month next year, the last ten-days period, when ground cauline leaf starts the withered and yellow time of falling seedling, withering cauline leaf in cleaning field, and earths up, and producing region claims " ulling up winter medicine ".
3.2.2.3 rich water quality management controls to rise from mid-September with growth, and used 1 excrement clear water every 15 ~ 20 days and carry seedling, totally 3 times, amount of application is respectively: 1000kg/ mu, 1000kg/ mu, 2000kg/ mu; After turning green in 2 ~ March of next year, then enrich 1 excrement clear water, consumption is 1000kg/ mu.
Early April pinches to the acrial part pinching that growing way is prosperous.
3.2.3 pick and process
3.2.3.1 late April of Second Year after cultivation of gathering gathers.Select fine day, dig out rhizome, shake off earth, removing cauline leaf.Slightly to dry in the air in field aqueous vapor, transport drying back.
3.2.3.2 Habitat producing dries.
3.3 detect
1 increment product are adopted in each community, and every increment product get 30 strains at random.Unification selects impurity elimination after gathering, section, dries, and pulverizes, and crosses 60 mesh sieves, makes medicinal powder, and trust academy of agricultural sciences of Sichuan Province has carried out heavy metal Cadmium detrmination.
Ethanol soluble extractives assay measures according to method under " Chinese Pharmacopoeia " 2010 editions Ligusticum wallichii items.
Ferulaic acid content measures and measures with reference to method under " Chinese Pharmacopoeia " 2010 editions Ligusticum wallichii items.
Chromatographic condition chromatographic column is KromasilC18 (5 μm, 250mm × 4.6mm): mobile phase is methyl alcohol-1% acetum (30: 70); Determined wavelength 321nm; Flow velocity 1.0mlmin
-1, column temperature 30 DEG C, sample size 10 μ l.
The preparation precision of reference substance solution takes forulic acid reference substance 100 μ g, puts in the brown volumetric flask of 5ml, adds 70% methyl alcohol and dissolve and be settled to scale, shake up, and obtaining concentration is 20.0 μ gml
-1reference substance solution.
Each sample medicinal powder (crossing No. four sieves) 0.5g is got in the preparation of sample solution, accurately weighed, puts in tool plug conical flask, precision adds 70% methyl alcohol 50ml, weighed weight after close plug, heating water bath backflow 30min, let cool, more weighed weight, the weight of less loss is supplied with 70% methyl alcohol, shake up, leave standstill, get supernatant, miillpore filter (0.45 μm) filters, get subsequent filtrate, to obtain final product.
The drafting precision of calibration curve draws reference substance solution 2,5,10,15,20 μ l sample introduction, and with forulic acid integrating peak areas value for ordinate, its content is abscissa, drawing standard curve.Regression equation is y=27.8848648x-1.1489417, r=0.9999, and ferulaic acid content is good linear relation with integrating peak areas value within the scope of 0.04 ~ 0.50 μ g.
Sample size measures accurate pipette samples solution 10 μ l sample introduction, by above-mentioned chromatographic condition working sample content.
The chromatogram of forulic acid reference substance and the chromatogram of Ligusticum wallichii test sample are as Fig. 1 ~ Fig. 2.
Data acquisition Microsoft Excel 2013 and SPSS 13 carries out adding up and Treatment Analysis.
4 testing results
Through detecting, the Ligusticum wallichii cadmium content of each group is as shown in the table:
Table 1 quicklime process experimental result (
n=3)
The results show, adopt the present invention to add the mode of the quicklime of 200kg/ mu, significantly can reduce the cadmium content of Ligusticum wallichii, reduction amplitude is 8.7%, effectively can improve the active ingredient extract of Ligusticum wallichii and the content of forulic acid simultaneously.
Comparative example 1
1 material
With embodiment 1.
2. region
With embodiment 1.
3 cultivation methods
Addition except quicklime is except 50kg/ mu, and all the other steps are identical with embodiment 1.
4 testing results
Testing result is as shown in table 2 below:
Table 2 quicklime process experimental result (
n=3)
Through detecting, when the addition of quicklime is 50kg/ mu, the cadmium content of Ligusticum wallichii can rise on the contrary, and the content of active ingredient extract and forulic acid all can decline.
Comparative example 2
1 material
With embodiment 1.
2. region
With embodiment 1.
3 cultivation methods
Addition except quicklime is except 150kg/ mu, and all the other steps are identical with embodiment 1.
4 testing results
Testing result is as shown in table 3 below:
Table 3 quicklime process experimental result (
n=3)
Through detecting, when the addition of quicklime is 150kg/ mu, the cadmium content of Ligusticum wallichii can rise on the contrary, and the content of active ingredient extract slightly declines, and the content of active ingredient forulic acid raises.
Comparative example 3
1 material
With embodiment 1.
2. region
With embodiment 1.
3 cultivation methods
Addition except quicklime is except 250kg/ mu, and all the other steps are identical with embodiment 1.
4 testing results
Testing result is as shown in table 4 below:
Table 4 quicklime process experimental result (
n=3)
Through detecting, when the addition of quicklime is 250kg/ mu, the cadmium content of Ligusticum wallichii can rise on the contrary, the slightly excellent decline of content of active ingredient extract, and the content of active ingredient forulic acid raises.
To sum up, the present invention, by adding the quicklime of specified quantitative, significantly can reduce the cadmium content of Ligusticum wallichii, effectively can improve the active ingredient extract of Ligusticum wallichii and the content of forulic acid simultaneously, and add the quicklime outside particular range of the present invention, then cannot reduce cadmium content.
Claims (6)
1. reduce a cultivation method for Ligusticum wallichii cadmium content, it is characterized in that: comprise the following steps:
A, soil treatment: preparing quick lime, mix with table soil, the usage amount of quicklime is 200kg/ mu;
B, get Ligusticum wallichii Siberian cocklebur kind, cultivation.
2. cultivation method according to claim 1, is characterized in that: in step b, and the step of described cultivation is as follows:
(1) Ligusticum wallichii Siberian cocklebur kind is got, sowing;
(2) fill the gaps with seedlings, intertill and clean tillage, fertilising;
(3) gather, dry.
3. cultivation method according to claim 2, is characterized in that: in step (1), and the time of described sowing is mid or late August.
4. cultivation method according to claim 2, is characterized in that: in step (1), and the seeding quantity of described Ligusticum wallichii Siberian cocklebur kind is every mu of 30 ~ 40kg.
5. cultivation method according to claim 2, it is characterized in that: in step (2), the mode of fertilising is: rise from mid-September, used 1 excrement clear water every 15 ~ 20 days, totally 3 times, amount of application is respectively: 1000kg/ mu, 1000kg/ mu, 2000kg/ mu; After turning green in 2 ~ March of next year, then enrich 1 excrement clear water, consumption is 1000kg/ mu.
6. cultivation method according to claim 1, is characterized in that: in step b, and the region of cultivation is Ao Ping town, Pengzhou City, Sichuan Province, and its longitude is east longitude 103 ° 59 ' 16.53 ", latitude is north latitude 31 ° 0.4 ' 52.33 ".
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114793807A (en) * | 2022-05-07 | 2022-07-29 | 四川省中医药科学院 | Planting method for reducing cadmium and improving quality of ligusticum wallichii |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102027855A (en) * | 2010-10-25 | 2011-04-27 | 赤峰天奇制药有限责任公司 | Technology for processing coastal glehnia root medicinal materials in planting and producing area and quality standard |
-
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- 2015-07-20 CN CN201510428524.5A patent/CN104938205A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102027855A (en) * | 2010-10-25 | 2011-04-27 | 赤峰天奇制药有限责任公司 | Technology for processing coastal glehnia root medicinal materials in planting and producing area and quality standard |
Non-Patent Citations (4)
| Title |
|---|
| 宁梓君等: "《改良酸性土壤对土壤活性态镉及川芎镉含量影响的研究》", 《中药材》 * |
| 宁梓君等: "《改良酸性土壤对川芎药用质量的影响》", 《中国当代医药》 * |
| 熊丙全等: "《川产道地药材川芎优质高产栽培技术》", 《四川农业科技》 * |
| 韩春梅: "四川地区川芎的高产栽培技术》", 《中药材》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114793807A (en) * | 2022-05-07 | 2022-07-29 | 四川省中医药科学院 | Planting method for reducing cadmium and improving quality of ligusticum wallichii |
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