CN105145602A - Pyraclostrobin and propineb compound wettable powder and preparation method thereof - Google Patents
Pyraclostrobin and propineb compound wettable powder and preparation method thereof Download PDFInfo
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- CN105145602A CN105145602A CN201510623953.8A CN201510623953A CN105145602A CN 105145602 A CN105145602 A CN 105145602A CN 201510623953 A CN201510623953 A CN 201510623953A CN 105145602 A CN105145602 A CN 105145602A
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Abstract
The invention discloses pyraclostrobin and propineb compound wettable powder and a preparation method thereof, and relates to the technical field of downy mildew prevention and treatment of grapes and ornamental flowers. The pyraclostrobin and propineb compound wettable powder is prepared from 5 parts of pyraclostrobin, 40 parts of propineb, 10 parts of fatty alcohol-polyoxyethylene ether, 10 parts of pesticide emulsifier 1600# and 35 parts of light calcium carbonate. The raw materials are mixed, coarse grinding and comminution by gas stream are conducted in sequence, then sufficient mixing is carried out, sampling analysis is performed, metering is conducted after products are qualified, and the products are packaged and put in storage. According to the pyraclostrobin and propineb compound wettable powder and the preparation method thereof, the pyraclostrobin and the propineb are mixed with the compounded 45% pyraclostrobin and propineb compound wettable powder at the ratio of 1 to 8, an obvious synergistic effect can be achieved, and the obvious synergistic effect cannot be achieved through other ratio mixed preparations.
Description
Technical field
The present invention relates to the downy mildew Prevention Technique field of ornamental flower, specifically relate to a kind of pyraclostrobin and Propineb built wettable powder and preparation method thereof.
Background technology
Downy mildew is the plant disease caused by the Peronosporaceae in fungi, obligatory parasitic bacteria, mainly through air-flow, water, farming and insect transmission.Germ spore germination temperature is 6 ~ 10 DEG C, is suitable for infecting temperature 15 ~ 17 DEG C, the flowers such as tree peony, Chinese herbaceous peony, Chinese rose, chrysanthemum of mainly causing harm, it cause harm wide in variety, the scope that infects is wide, almost there is generation in the whole nation.Downy mildew is general first falls ill from lower blade, and their early stage produces the water stain shape point of light green, and scab edge is not obvious, and Later development is yellow irregular scab, and during high humidity, blade back produces the mould layer of canescence, gradually becomes Dark grey.
At present, the bactericide that the existing downy mildew for ornamental flower is prevented and treated, such as Chinese patent " CN101647446A " discloses one " bactericidal composition containing fluopicolide and pyraclostrobin ", adopts weight ratio to be that the fluopicolide of 1:30 ~ 10:1 and pyraclostrobin carry out composite." CN102239864A " discloses one " bactericidal composition containing Propineb and pyraclostrobin ", adopts weight ratio to be that the Propineb of 50:1 ~ 1:80 and pyraclostrobin carry out composite separately Chinese patent.
Inventor verifies through long term test, and this above-mentioned several bactericide mainly exists following defect:
1), by several concrete proportioning disclosed in above-mentioned several bactericide test, find cannot play good control efficiency to the control of the downy mildew of ornamental flower.
2), for several formulations disclosed in above-mentioned several bactericide, find through verification experimental verification the defect that all there is different problem.Such as, the wetting powder not easily dispersion suspension in water of preparation, or blocking shower nozzle, the phenomenon such as reason precipitation in sprayer, cause spray irregular.
3), composite problem, by several concrete proportioning disclosed in above-mentioned several bactericide carry out virulence test, heat storage stability test and mixture co-toxicity coefficient measure find, all cannot reach obvious synergistic effect.
Summary of the invention
An object of the present invention is to provide a kind of pyraclostrobin and Propineb built wettable powder, and this wetting powder is reasonable in design, good to the control efficiency of the downy mildew of ornamental flower, and can realize the notable synergistic effect of pyraclostrobin and Propineb.
For achieving the above object, present invention employs following technical scheme:
A kind of pyraclostrobin and Propineb built wettable powder, be made up of the component of following weight portion:
Pyraclostrobin is New-type wide-spectrum bactericide, and its mechanism of action is mitochondrial respiratory inhibitor, namely by stoping electro transfer in cytochrome synthesis, has protection, treatment, blade infiltration conduction.Pyraclostrobin missible oil shows there is good control efficiency to powdery mildew of cucumber, downy mildew and banana freckle, leaf spot, stalk break etc. through field control effectiveness test result.
Propineb is a kind of wide spectrum, quick-acting protective fungicides; its bactericidal mechanism is the oxidation suppressing pyruvic acid in cause of disease thalline; it is the protective fungicide of long, wide spectrum of a kind of quick-acting, longevity of residure; morning of the downy mildew of the crop such as vine, vegetables and tomato and potato, late blight are all had to good protective effect, and also have certain inhibitory action to the germ disease of powdery mildew, rust and Botrytis.To crop safety under recommended dose.By Chinese pesticide toxicity grading criteria, Propineb belongs to low toxicity bactericide.
Pyraclostrobin of the present invention and Propineb built wettable powder, its beneficial effect shows:
1) wetting powder that, prepared by the present invention is all obviously better than the product prepared by other proportionings in suspensibility, wet screening test etc. and heat storage stability etc.
2), verify by experiment, pyraclostrobin and Propineb with 1:8 proportioning mixed composite 45% time pyraclostrobin and Propineb built wettable powder, notable synergistic sphere of action can be played, and the preparation that other proportioning is mixed, all cannot reach notable synergistic sphere of action.Meanwhile, the actual toxicity of different ratio does not promote along with the enhancing of theoretical toxicity, there is no regular change between actual toxicity and theoretical toxicity.
Another object of the present invention is to the preparation method that a kind of pyraclostrobin and Propineb built wettable powder are provided, raw material is mixed, successively through coarse crushing and air-flow crushing, more fully mixes, sample analysis, qualified rear metering, packaging, warehouse-in.
The preparation method of pyraclostrobin of the present invention and Propineb built wettable powder, preparation technology is comparatively easy, is easy to suitability for industrialized production.
Embodiment
Below with reference to embodiment, the present invention is described in detail.But; embodiment content is only citing made for the present invention and explanation; affiliated those skilled in the art make various amendment to described specific embodiment or supplement or adopt similar mode to substitute; only otherwise depart from the design of invention or surmount this scope as defined in the claims, protection scope of the present invention all should be belonged to.
One, the preparation of pyraclostrobin and Propineb built wettable powder, and testing result, the heat storage stability result of the test of all technical of wetting powder prepared by each embodiment.
Embodiment 1
45% pyraclostrobin and Propineb built wettable powder, each component and weight portion as follows:
| Composition | Weight portion |
| Pyraclostrobin | 5 |
| Propineb | 40 |
| Fatty alcohol-polyoxyethylene ether | 10 |
| Phenethyl phenol polyoxyethylene poly-oxygen propylene aether | 10 |
| Precipitated calcium carbonate | 35 |
Preparation method: raw material is mixed, successively through coarse crushing and air-flow crushing, more fully mixes, sample analysis, qualified rear metering, packaging, warehouse-in.
Embodiment 2
46% pyraclostrobin and Propineb built wettable powder, each component and weight portion as follows:
| Composition | Weight portion |
| Pyraclostrobin | 5 |
| Propineb | 41 |
| Fatty alcohol-polyoxyethylene ether | 11 |
| Phenethyl phenol polyoxyethylene poly-oxygen propylene aether | 9 |
| Precipitated calcium carbonate | 34 |
Preparation method is with embodiment 1.
Embodiment 3
44% pyraclostrobin and Propineb built wettable powder, each component and weight portion as follows:
| Composition | Weight portion |
| Pyraclostrobin | 6 |
| Propineb | 38 |
| Fatty alcohol-polyoxyethylene ether | 11 |
| Phenethyl phenol polyoxyethylene poly-oxygen propylene aether | 11 |
| Precipitated calcium carbonate | 34 |
Preparation method is with embodiment 1.
Embodiment 4
43% pyraclostrobin and Propineb built wettable powder, each component and weight portion as follows:
| Composition | Weight portion |
| Pyraclostrobin | 4 |
| Propineb | 39 |
| Fatty alcohol-polyoxyethylene ether | 9 |
| Phenethyl phenol polyoxyethylene poly-oxygen propylene aether | 11 |
| Precipitated calcium carbonate | 37 |
Preparation method is with embodiment 1.
Embodiment 5
47% pyraclostrobin and Propineb built wettable powder, each component and weight portion as follows:
| Composition | Weight portion |
| Pyraclostrobin | 5 |
| Propineb | 42 |
| Fatty alcohol-polyoxyethylene ether | 11 |
| Phenethyl phenol polyoxyethylene poly-oxygen propylene aether | 9 |
| Precipitated calcium carbonate | 33 |
Preparation method is with embodiment 1.
Pyraclostrobin prepared by embodiment 1 ~ 5 and Propineb built wettable powder, the testing result of product all technical is as shown in table 1, and product heat storage stability result of the test is as shown in table 2.
The testing result of a table 15 embodiment preparing product all technical
A table 25 embodiment preparing product heat storage stability result of the test
Can be found out by table 1 and 2, product prepared by embodiment 1 is all obviously better than the product prepared by other embodiments in suspensibility, wet screening test etc. and heat storage stability etc.
Two, toxicological information
Rat acute Oral toxicity is tested:
Female male SD rat acute per os LD
50all be greater than 5000mg/kgb.wt..The toxicity of this sample to female male SD rat belongs to hypotoxicity.
Rat acute percutaneous toxicity test:
Female male SD rat acute is through skin LD
50value is all greater than 2000mg/kgb.wt..This sample all belongs to the toxicity such as low to female male SD rat.
Rat acute toxicity on inhalation is tested:
Female male SD rat acute sucks LC
50all be greater than 2000mg/m
3.This sample all belongs to the toxicity such as low to female male SD rat.
Guinea pig skin irritant test:
Skin irritatin mean scores is 0, and cavy is detected district and has no any skin reaction, and this sample is to guinea pig skin nonirritant.
Rabbits with Acute eye irritation is tested:
The former medicine of submitted sample is 3 to rabbit eye irritation score index, and average eye irritation is that 0 (after 48h), 48h recover normal, to eye nonirritant.
Guinea pig skin allergy is tested
Adopt partial closure to be coated with pico farad, detection zone has no any skin reaction, sensitization rate (%)=0, and according to sensitization strength grading standard, this sample belongs to weak sensitizer.
According to China's pesticide toxicity grading criteria, 45% pyraclostrobin Wettable propineb powder belongs to hypotoxicity agricultural chemicals.
Three, measure for the composite indoor biological activity (virulence) of the pyraclostrobin and Propineb of viewing and admiring chrysanthemum downy mildew
1, experiment purpose
Be intended to measure pyraclostrobin, Propineb and the two different proportion proportioning to the virulence of viewing and admiring chrysanthemum downy mildew, to judge that the two different ratio is viewed and admired chrysanthemum downy mildew to suppression and whether had synergistic effect.
2, experimental condition
2.1 for examination target
View and admire chrysanthemum Pseudoperonospora cubensis (PowderyMildews) test strain, provided by Zhi Bing teaching and research room of Agricultural University Of Anhui.
2.2 condition of culture
By the Pseudoperonospora cubensis strain transfer of indoor 4 DEG C of Refrigerator stores on PSA medium, cultivate in 25 DEG C of incubators and make it activate, for subsequent use after twice switching.
2.3 instrument and equipment
Electronic balance (sensibility reciprocal 0.1mg), test tube, culture dish, pressure steam sterilizer, superclean bench, biochemical cultivation case, pipettor, inoculator, card punch (internal diameter is 5mm) etc.
3, experimental scheme
3.1 examination materials prepare
Pseudoperonospora cubensis pathogen is placed in biochemical cultivation case and cultivates, for subsequent use.
3.2 medicament
The former medicine of pyraclostrobin of content 97.5%, the former medicine of Propineb of content 85%, provides by former medicine portion of Anhui Meilan Agricultural Development Co., Ltd..
3.3 medicament preparations
5 series mass concentration are diluted to the medicament that embodiment 1-5 is prepared by acetone.
4, test method
Carry out with reference to " farm-chemical indoor determination test rule----bactericide " (NY/T1156.6-2006).In order to grope the activity of each medicament to strains tested, first carry out preliminary experiment.Cultivate be placed on the medium containing higher and low concentration medicament by mycelia under, estimate EC
50, and then according to the EC estimated
50value, is made into its EC by medium
50the pastille medium of the variable concentrations gradient of left and right is cultivated.
This test adopts plate mycelial growth rate method to measure medicament to the virulence of Pseudoperonospora cubensis.Concrete grammar is as follows: through the Pseudoperonospora cubensis bacterial strain PSA medium culture of switching activation, when bacterium colony grows to culture dish 3/4ths size, punch from edge with the card punch that internal diameter is 5mm, the mycelia block broken into is as inoculum.Respectively medicament mother liquor is added in the PSA medium of people's sterilizing thawing, fully shake up, make medicament ultimate density (calculating by active ingredient) reach variable concentrations gradient (table 1).About 15mL pastille medium poured into by every ware, if not dosing is contrast, often processes 4 repetitions.Move the mycelia block (diameter 5mm) connecing new growth central in flat board, be placed in 25 DEG C of incubators and cultivate 2d (Pseudoperonospora cubensis), 7d (Pseudoperonospora cubensis) and 15d (Pseudoperonospora cubensis).Measure colony diameter by right-angled intersection method, calculate each process net growth, mycelial growth inhibition rate.
In formula: bacterium dish diameter is 5mm.
5, data statistic analysis
Mycelial growth inhibition rate is converted into probit value (y), and drug concentration (μ g/mL) converts logarithm value (x) to, tries to achieve virulence regression equation (y=a+bx) with method of least squares.With DPS statistical software, regression analysis is carried out to the log concentration value of each single dose and different mixture and corresponding inhibiting rate probit value, calculate EC
50value and 95% confidence limit.
According to EC
50calculate the actual measurement toxicity index (ATI) of built agent, theoretical toxicity index (TTI) and co-toxicity coefficient (CTC).
Built agent actual measurement toxicity index (ATI)=single dose EC
50/ built agent EC
50× 100
Theoretical toxicity index (TTI)=A toxicity index × A content (%)+B toxicity index × B content (the %)+C toxicity index × C content (%) in built agent in built agent in built agent of built agent
Co-toxicity coefficient (CTC)=ATI/TTI × 100
Calculate medicament different ratio synergy ratio (CTC) according to the abundant method of Sun Yun, CTC≤80 are antagonism, and 80 < CTC < 120 are summation action, and CTC >=120 are synergistic effect.
6, results and analysis
Wetting powder prepared by embodiment 1 shows as notable synergistic effect (co-toxicity coefficient reaches 275) to the co-toxicity viewing and admiring chrysanthemum downy mildew, and wetting powder prepared by embodiment 2-5 only shows as addition (co-toxicity coefficient is followed successively by 80,92,87,95) to the co-toxicity viewing and admiring chrysanthemum downy mildew.
Claims (2)
1. pyraclostrobin and a Propineb built wettable powder, is characterized in that: this wetting powder is made up of the component of following weight portion:
2. prepare a method for pyraclostrobin and Propineb built wettable powder as claimed in claim 1, it is characterized in that: raw material is mixed, successively through coarse crushing and air-flow crushing, more fully mixes, sample analysis, qualified rear metering, packaging, warehouse-in.
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|---|---|---|---|
| CN201510623953.8A CN105145602A (en) | 2015-09-25 | 2015-09-25 | Pyraclostrobin and propineb compound wettable powder and preparation method thereof |
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|---|---|
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|---|---|---|---|
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102217620A (en) * | 2011-05-06 | 2011-10-19 | 浙江泰达作物科技有限公司 | Water dispersible granules of bactericide compounded by propineb and pyraclostrobin and preparation method thereof |
| CN102283228A (en) * | 2010-06-19 | 2011-12-21 | 海利尔药业集团股份有限公司 | Sterilization composite containing pyraclostrobin and propineb |
| CN102302025A (en) * | 2011-03-12 | 2012-01-04 | 陕西汤普森生物科技有限公司 | Bactericidal composition containing pyraclostrobin and thiocarbamate |
| CN103260410A (en) * | 2010-12-08 | 2013-08-21 | 巴斯夫欧洲公司 | Pesticidal mixtures |
| CN103548857A (en) * | 2013-08-21 | 2014-02-05 | 陕西康禾立丰生物科技药业有限公司 | Pyraclostrobin-containing pesticide composition |
-
2015
- 2015-09-25 CN CN201510623953.8A patent/CN105145602A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102283228A (en) * | 2010-06-19 | 2011-12-21 | 海利尔药业集团股份有限公司 | Sterilization composite containing pyraclostrobin and propineb |
| CN103260410A (en) * | 2010-12-08 | 2013-08-21 | 巴斯夫欧洲公司 | Pesticidal mixtures |
| CN102302025A (en) * | 2011-03-12 | 2012-01-04 | 陕西汤普森生物科技有限公司 | Bactericidal composition containing pyraclostrobin and thiocarbamate |
| CN102217620A (en) * | 2011-05-06 | 2011-10-19 | 浙江泰达作物科技有限公司 | Water dispersible granules of bactericide compounded by propineb and pyraclostrobin and preparation method thereof |
| CN103548857A (en) * | 2013-08-21 | 2014-02-05 | 陕西康禾立丰生物科技药业有限公司 | Pyraclostrobin-containing pesticide composition |
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Application publication date: 20151216 |
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