CN106508970B - A kind of combination sterilization composition and its application - Google Patents
A kind of combination sterilization composition and its application Download PDFInfo
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- CN106508970B CN106508970B CN201610980421.4A CN201610980421A CN106508970B CN 106508970 B CN106508970 B CN 106508970B CN 201610980421 A CN201610980421 A CN 201610980421A CN 106508970 B CN106508970 B CN 106508970B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
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Abstract
The invention discloses a kind of combination sterilization composition and its application, the composition is prepared by the raw material of following mass percent: eugenol 0.1%-5%, toVerticilliumdahliaActivitie Activitie S of Relative albumen 0.01%-8%, and surplus is customary adjuvant additive.The present invention by indoor toxicity determination test and field trial determine biogenic pesticide eugenol and toVerticilliumdahliaActivitie Activitie S of Relative albumen be used alone and compounding after to the control effect of downy mildew of garpe, establish the optimal proportion of two biogenic pesticides, for producing and using, to achieve the purpose that reduce Pesticide environment pollution and germ drug resistance occurrence and development；Wherein, synergistic effect is shown in the ratio range of eugenol and toVerticilliumdahliaActivitie Activitie S of Relative albumen 1:15-25:1, wherein the proportion synergy with 1:6 is maximum, coefficient of synergism 1.89；Biogenic pesticide eugenol is different with the toVerticilliumdahliaActivitie Activitie S of Relative albumen mode of action, after compounding is not easy that germ is made to develop drug resistance, does not easily cause environmental pollution.
Description
Technical field
The present invention relates to biological source agricultural bactericide fields, and in particular to a kind of combination sterilization composition and its application.
Background technique
Downy mildew of garpe is Major Diseases on grape, when grape leaf curtain is overstocked, rainfall is more, vineyard impeded drainage when it is easy
Morbidity, when the continuous rainy weather of season in spring and autumn is that morbidity is heavier.Downy mildew of garpe main harm grape leave and fruit, morbidity are tight
The obvious underproduction is caused when weight.
Production at present is upper mainly to use chemical pesticide control downy mildew, and the application of a large amount of chemical pesticides leads to agro-ecosystem
The destruction of system, at the same bring controlling object drug resistance, chemical pesticide residual the problems such as.With food-safe more next of the public
More pay attention to, the increasingly gradually high cry of global protection environment, developing and utilizing has favorable compatibility with environment, harmless to the mankind
Biological pesticide resource also become the research emphasis and hot spot of scientific research personnel.
Biogenic pesticide is also known as biological pesticide or biological source natural products pesticide, refers to and directly utilizes biological living or biology
The biologically active substance generated in metabolic process or the pesticide from organism extraction of substance as prevention and treatment pest and disease damage, have
Effect ingredient is naturally occurring, with less toxic, pollution-free, specificity is strong, be easy to degrade, accumulation toxicity may in ecological environment
Property is small, has the characteristics that Environmental compatibility is good.Biogenic pesticide has guarantee effect to organic food and pollution-free food production, keeps away
Exempt from or mitigate to adversely affect using chemical pesticide, and have many advantages, such as that pathogen is made to be difficult to develop drug resistance, is ideal
Pesticide type.Eugenol belongs to botanical pesticide and is present in caryophyllus oil, cinnamon leaves oil, cassia oil, camphorated oil, mace oil
Etc. various plants oil in.Eugenol can have upward conduction function and safe and environment-friendly by the leaf, stem, root absorption of plant
Noresidue, medicine effect for treating is rapid, and the lasting period is long.ToVerticilliumdahliaActivitie Activitie S of Relative activator protein belongs to microprotein pesticide, mainly passes through
Improve the immune system of plant itself and activated plant to the resistance of plant disease.
It in agricultural production real process, is compounded by different cultivars ingredient, is controlling disease, reduces the normal of resistance
Square method.In most cases, the compounding effect of pesticide is all additive effect, really has the compounding of synergistic effect seldom.Through
Inventor's research is crossed, discovery compounds mutually eugenol with toVerticilliumdahliaActivitie Activitie S of Relative activator protein, has in a certain range well
Synergistic effect, improve grape to downy mildew of garpe anti pathologic immunity power while play good therapeutic effect, and at present about
Eugenol compounding related to toVerticilliumdahliaActivitie Activitie S of Relative activator protein is for preventing and treating downy mildew of garpe, at present both at home and abroad there is not yet related
Report.
Summary of the invention
To solve the above problems, the composition has height the present invention provides a kind of combination sterilization composition and its application
It the advantages that effect, low toxicity, low-residual, is prevented and treated especially suitable for downy mildew of garpe.
To achieve the above object, the technical scheme adopted by the invention is as follows:
A kind of combination sterilization composition is prepared by the raw material of following mass percent: eugenol 0.1%-5%,
ToVerticilliumdahliaActivitie Activitie S of Relative albumen 0.01%-8%, surplus are customary adjuvant additive.
Preferably, the eugenol and toVerticilliumdahliaActivitie Activitie S of Relative albumen quality proportion be 25:1-1:25, enhancing proportions 1:
15-25:1。
Preferably, the eugenol and toVerticilliumdahliaActivitie Activitie S of Relative albumen quality proportion are 1:6.
Above-mentioned combination sterilization composition can be used for preparing the drug of prevention and treatment downy mildew of garpe.
The invention has the following advantages:
Biogenic pesticide eugenol and toVerticilliumdahliaActivitie Activitie S of Relative egg are determined by indoor toxicity determination test and field trial
To the control effect of downy mildew of garpe after white exclusive use and compounding, the optimal proportion of two biogenic pesticides is established, for production
It uses, to achieve the purpose that reduce Pesticide environment pollution and germ drug resistance occurrence and development；Wherein, eugenol and superfine chain
Synergistic effect is shown in the ratio range of lattice spore albumen 1:15-25:1, wherein the proportion synergy with 1:6 is maximum, synergy system
Number is 1.89；Biogenic pesticide eugenol is different with the toVerticilliumdahliaActivitie Activitie S of Relative albumen mode of action, is not easy to generate germ after compounding
Drug resistance does not easily cause environmental pollution.
Specific embodiment
In order to which objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
It is described in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
The embodiment of the invention provides a kind of combination sterilization compositions, are prepared by the raw material of following mass percent:
Eugenol 0.1%-5%, toVerticilliumdahliaActivitie Activitie S of Relative albumen 0.01%-8%, surplus are customary adjuvant additive.
The eugenol and toVerticilliumdahliaActivitie Activitie S of Relative albumen quality proportion are 25:1-1:25, enhancing proportions 1:15-25:
1, the optimum quality proportion of the eugenol and toVerticilliumdahliaActivitie Activitie S of Relative albumen is 1:6.
Embodiment 1
Indoor bioassay test
Test target
Plasmopara viticola (Plasmopara viticola), strain is picked up from field.
Test material prepares
Grape susceptible variety rarity is selected, from up to down 4 leaf positions~6 leaf positions, clip same area, growing way are consistent, have
The blade of 1cm~2cm petiole is placed in culture dish with wet cotton balls package petiole, and moisturizing is spare.
Test medicine
ToVerticilliumdahliaActivitie Activitie S of Relative albumen raw medicine, Institute of Plant Protection, the Chinese Academy of Agricultural Sciences Langfang pesticide pilot plant provide, 98% eugenol, river
Soviet Union's Jintan City Wade dragon Chemical Co., Ltd. product；1.4% eugenol toVerticilliumdahliaActivitie Activitie S of Relative albumen composite bactericide, Shanxi Province
Academy of agricultural sciences's fruit tree research is prepared.
Comparison medicament
98% dimethomorph raw medicine, ShenYang, Liaoning Province good harvest pesticide Co., Ltd product.
Test procedure
S1, sporangia suspension are prepared
Field natural occurrence blade is selected, inferior lobe piece back side Pseudoperonospora cubensis sporangium is washed with 4 DEG C of distillations, is made into suspension
(concentration is controlled at every milliliter 1 × 105A~1 × 107A sporangium), it stores at 4 DEG C spare.
S2, medicament are prepared
By several to 0.05% Tween 80 aqueous solution dissolved dilution of reagent agent, it is spare to be made into 10000mg/L mother liquor.Often
5 series mass concentration are arranged in a medicament.
S3, chemicals treatment
Medical fluid is uniformly sprayed on vacuum side of blade, after medical fluid natural air drying, by each processing blade and blade backwards to upper, by processing
It is emitted in moisture preservation box after label；Test sets the processing without medicament and makees blank control.
S4, inoculation and culture
Vacuum side of blade is inoculated in ready 10 microlitres of Fresh spores capsule suspension drop.Every blade inoculation 4 drips, every place
Reason is no less than 5 leaves.Protectiveness test is inoculated with for 24 hours after pharmaceutical treatment, and curative tests are inoculated with for 24 hours before chemicals treatment.It connects
Ware lid is covered after kind, growth cabinet is placed in or has the humidistat of illumination, in daily continuous illumination/dark 12h alternating, temperature is
It 22 DEG C, cultivates under conditions of 90% or more relative humidity.5~7d experimental investigation result.
Record lesion diameter is measured depending on blank control incidence, unit is millimeter (mm).
Data statistics and analysis
According to survey data, control efficiency is calculated by formula (1), is indicated with percentage (%), calculated result retains decimal
Two after point.
In formula: P-control efficiency；D0- blank control lesion diameter；D1- chemicals treatment lesion diameter.
Make regression analysis according to each drug concentration logarithm and corresponding preventive effect probit value, calculates the EC of each medicament50、EC90
Equivalent and its 95% confidence limit.
When carrying out drug combination toxicity test, according to Wadley method or Sun Yunpei's Method calculate mixture coefficient of synergism (SR) or
Co-toxicity coefficient (CTC) evaluates the synergy type of mixture.
Wadley method: evaluating the synergistic effect of pharmacy mix according to coefficient of synergism (SR), i.e. SR < 0.5 is antagonism,
0.5≤SR≤1.5 are summation action, and SR > 1.5 are synergistic effect.Coefficient of synergism (SR) is calculated by formula (2), (3):
In formula:
X1The EC of-mixture50Theoretical value, unit are milligrams per liter (mg/L)；
PAThe percentage composition of A in-mixture, unit are percentage (%)；
PBThe percentage composition of B in-mixture, unit are percentage (%)；
The EC of A in A-mixture50Value, unit are milligrams per liter (mg/L)；
The EC of B in B-mixture50Value, unit are milligrams per liter (mg/L).
In formula:
SR-mixture coefficient of synergism；
X1-mixture EC50Theoretical value, unit are milligrams per liter (mg/L)；
X2-mixture EC50Measured value, unit are milligrams per liter (mg/L).
Test result
Pass through two kinds of single doses of Laboratory Evaluation eugenol and toVerticilliumdahliaActivitie Activitie S of Relative albumen and its different ratio mixture system
Control efficiency of the agent to downy mildew of garpe, the results showed that show good inhibition effect in two kinds of single dose rooms to downy mildew of garpe
Fruit, EC50Respectively 0.6912mg/L and 2.4564mg/L.The EC of the common medicament dimethomorph of field control downy mildew50For
5.4923mg/L.Both eugenol and toVerticilliumdahliaActivitie Activitie S of Relative albumen effective component ratio show synergy when being 1:15-25:1
Effect, especially the most significant with 1:6 proportion synergy, EC50For 0.7932mg/L, coefficient of synergism is that 1.89. illustrates 1.4% fourth
Fragrant phenol toVerticilliumdahliaActivitie Activitie S of Relative albumen composite bactericide has very high bactericidal activity to downy mildew of garpe.
1 eugenol of table and toVerticilliumdahliaActivitie Activitie S of Relative albumen optimum proportioning and comparison medicament Antibacterial Activity
Embodiment 2
Field control effectiveness test
According to eugenol and toVerticilliumdahliaActivitie Activitie S of Relative albumen optimum compound proportion example 1:6 that lab screening goes out, it is configured with 1.4% fourth
Eugenol and toVerticilliumdahliaActivitie Activitie S of Relative albumen composite bactericide, wherein containing 0.2% eugenol and 1.2% toVerticilliumdahliaActivitie Activitie S of Relative albumen.Field
Between with 3% toVerticilliumdahliaActivitie Activitie S of Relative albumen WP 800 mixed, the 50% dimethomorph WP 1500 with 0.3% eugenol SL, 800 times of buckets again
Carry out field control effectiveness test as comparison medicament again.
Subjects are downy mildew.
Trial crops are grape, and test grape variety selects rarity.
Environmental condition
The cultivation of garden trellis type is tested, age of tree life in 9 years, downy mildew over the years occurs universal and uniform.(August 1 during test
Day-August 30 days) grape breeding time to young sprout growth period.All experimental plot cultivation conditions and tree body growing way are consistent, and with work as
The cultivation step on ground is consistent.
Experimental design and arrangement
Test medicine: 1.4% eugenol toVerticilliumdahliaActivitie Activitie S of Relative albumen composite bactericide, academy of agricultural sciences, Shanxi Province fruit tree research
It is prepared.
Comparison medicament:
3% toVerticilliumdahliaActivitie Activitie S of Relative albumen WP, Institute of Plant Protection, the Chinese Academy of Agricultural Sciences Langfang pesticide pilot plant product；
0.3% eugenol SL, Shandong Province hundred million praise agrochemical Co., Ltd product；
50% dimethomorph WP, German BASF Trading Co., Ltd product.
Cell arrangement
Test medicine, comparison medicament and the processing of the cell of blank control use random alignment.
The area of cell and repetition
Every cell vine is 15 plants, is repeated 4 times.
The design of 2 field control effectiveness test of table
| Processing number | Medicament | It is administered dosage |
| 1 | 1.4% eugenol toVerticilliumdahliaActivitie Activitie S of Relative albumen composite bactericide | 1000 times |
| 2 | 1250 times | |
| 3 | 1500 times | |
| 4 | 3% SL barrels of WP+0.3% eugenol of toVerticilliumdahliaActivitie Activitie S of Relative albumen is mixed | 800 times |
| 5 | 50% dimethomorph WP | 1500 times |
| 6 | Blank control |
Insecticide-applying way
Usage mode
It is watered by spraying, fills up institute's water requirement after first being dissolved with a small amount of water again；Make when spraying leave dual sides uniformly medicine, drop
Until water.
Use the type of instrument: backpack sprayer, injection diameter 0.8mm
The time of application and number
Field downy mildew of garpe occur early period be administered for the first time, spray altogether 3 times, specific spraying time be on August 1st, 2015,8
On the moon 8, August 18th.
Dosage and capacity: the medical fluid dosage 2249L/hm of per hectare2。
The data requirement for preventing and treating other pest and disease damage medicaments: during test, each cell does not spray it in addition to spraying test medicament
His any fungicide；Record the accurate data of this kind of pharmacy application.
20 current year pumpings of every cell random searching are newly climing, investigate whole blades from top to bottom, remember by following stage division
Record the sick numbers of sheets and total number of sheets at different levels.Blade stage division: 0 grade: disease-free spot；1 grade: lesion area account for the 5% of entire leaf area with
Under；3 grades: lesion area accounts for the 6%~25% of entire leaf area；5 grades: lesion area accounts for the 26%~50% of entire leaf area；7
Grade: lesion area accounts for the 51%~75% of entire leaf area；9 grades: lesion area accounts for 75% or more of entire leaf area.
Control time and number
Without downy mildew before medicine, 10d (August 28 days) investigates a drug effect after application.
Drug effect calculation method:
To the other influences of crop
Test medicine, comparison medicament to for examination grape variety safety, without phytotoxicity.
Test result
1.4% 1000 times of eugenol toVerticilliumdahliaActivitie Activitie S of Relative composite bactericide, 1250 times show good preventive effect, prevent
Effect is respectively 96.78%, 91.20%, is significantly higher than 1500 times of 50% dimethomorph WP, 3% toVerticilliumdahliaActivitie Activitie S of Relative in 5% level
The preventive effect of albumen SL barrels of mixtures of WP+0.3% eugenol, 1.4% 1500 times of eugenol toVerticilliumdahliaActivitie Activitie S of Relative composite bactericide
86.34% and two is not significantly different between control group, absolutely proves, 1.4% eugenol toVerticilliumdahliaActivitie Activitie S of Relative composite bactericide
To downy mildew of garpe have good bactericidal activity, control efficiency is suitable with chemical pesticide, can part substituting chemical pesticide, have
Have broad application prospects and Development volue.It is investigated during this, each processing is to for examination host plant safety, no phytotoxicity.
Table 3
Note: data are to manage average data everywhere in table.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the principle of the present invention, it can also make several improvements and retouch, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (2)
1. a kind of combination sterilization composition, which is characterized in that be prepared by the raw material of following mass percent: eugenol
0.1%-5%, toVerticilliumdahliaActivitie Activitie S of Relative albumen 0.01%-8%, surplus are customary adjuvant additive；The eugenol and superfine chain
Lattice spore albumen quality proportion is 1:6.
2. a kind of application of combination sterilization composition as described in claim 1, which is characterized in that be used to prepare prevention and treatment oenin
The drug of mildew.
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| CN111449065B (en) * | 2020-05-11 | 2021-03-26 | 枣庄学院 | Biopesticide for preventing and treating downy mildew |
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