CN105123506A - Tobacco seed oil medium pollen and preparation method and purpose thereof. - Google Patents
Tobacco seed oil medium pollen and preparation method and purpose thereof. Download PDFInfo
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- CN105123506A CN105123506A CN201510565007.2A CN201510565007A CN105123506A CN 105123506 A CN105123506 A CN 105123506A CN 201510565007 A CN201510565007 A CN 201510565007A CN 105123506 A CN105123506 A CN 105123506A
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Abstract
The invention provides tobacco seed oil medium pollen and a preparation method and a purpose thereof. According to the tobacco seed oil medium pollen, each milliliter of tobacco seed oil contains 25-50 milligram of pure tobacco pollen; the preparation method comprises the following steps: a physical squeezing method is employed for extracting the tobacco seed oil, impurities are filtered by a 60 mesh sieve, and the tobacco seed oil is placed at 4 DEG C for storage; b. according to a ratio that one milliliter of the tobacco seed oil is added with 25-50 milligram of the pure tobacco pollen, the seed oil and pollen are uniformly mixed for standby; the tobacco seed oil medium pollen is smeared on female parent stigmas of tobacco flowers, thereby carrying out a artificial pollination for tobacco hybridization seed production. Artificial pollination with the tobacco seed oil medium pollen can increase percentage of fertile fruit and improve seed output and quality; the tobacco seed oil has high toughness and good adhesive force to the tobacco stigma, so that the prepared tobacco seed oil medium pollen can be used for artificial pollination, thereby increasing pollination efficiency with good effect, effectively avoiding loss and waste, saving pollen amount, and effectively reducing seed production cost.
Description
Technical field
The invention belongs to plant hybridization pollination technique field, be specifically related to a kind of tobacco seed seed oil medium pollen, also relate to preparation method and the purposes of this medium pollen simultaneously.
Background technology
In tobacco male sterile line and cross breeding seed process, artificial pollination is an important and requisite job.Tobacco pollen amount is few, collect time-consuming taking a lot of work, and is pollen waste when reducing pollination, improves Pollen Activity and effective rate of utilization, in the tobacco hybridization production of hybrid seeds, generally become medium pollen to pollinate with the even mixture of appropriate medium pure for tobacco pollen.
Medium pollen comprises: solid medium pollen and liquid medium pollen pollination.Solid medium pollen pollination technique is comparatively ripe, Patents and application report more.In liquid medium pollen pollination technique field, the technology of employing spreads upon after mixing with pollen with the mixed solution of the composition such as boric acid, sucrose, the technology on plant flower column cap.Adopt this technology, because the plant pollen flow of water in different cultivars, period differs greatly, easily produce pollen plasmolysis or rise brokenly in a liquid, cause Pollen Activity to decline even inactivation; In addition, due to the mixed liquid medium pollen of solution and column cap adhesive force weak, the liquid medium pollen after pollination is easy to depart from from column cap run off, and causes that liquid medium pollen Pollination Effect is poor, seed production is low.
Tobacco seed seed oil is a kind of new vegetable oil in recent years developed, and is only applied to medical treatment, biofuel field at present, prepares the correlative study of medium pollen and apply and there is not been reported as liquid medium.
Summary of the invention
The object of the invention is to in prior art, there is pollen and easily produces plasmolysis at liquid or rise brokenly in liquid medium pollen, causes Pollen Activity to decline the problem of even inactivation; There is liquid medium pollen and column cap adhesive force is weak, liquid medium pollen after pollination is easy to depart from from column cap run off, cause the problem that liquid medium pollen Pollination Effect is poor, seed production is low, a kind of tobacco seed seed oil medium pollen and its production and use is provided, solves problems of the prior art.
Technical solution of the present invention is as follows:
A kind of tobacco seed seed oil medium pollen, contains tobacco pure pollen 25-50 milligram in every 1 milliliter of tobacco seed seed oil in this tobacco seed seed oil medium pollen.
Above-mentioned tobacco seed seed oil medium pollen, its preparation method comprises the following steps:
A. tobacco seed clear water is cleaned, dry to water content lower than 5%, adopt physical squeezing method to squeeze out tobacco seed seed oil, after considering impurity with 60 mesh sieves, save backup under being placed in 4 ° of C;
B. take the pure pollen of tobacco, add in tobacco seed seed oil, add the amount of the pure pollen of 25-50 milligram tobacco according to every 1 milliliter of tobacco seed seed oil, Homogeneous phase mixing.
Above-mentioned tobacco seed seed oil medium pollen, purposes is applied on the maternal column cap of tobacco flower, carries out tobacco hybridization production of hybrid seeds artificial pollination.
Through experimental results demonstrate, the present invention has following outstanding advantages:
1. adopt tobacco seed seed oil medium pollen to carry out artificial pollination, can percentage of fertile fruit be improved, and then improve seed production and quality;
2. tobacco seed seed oil toughness is high, and with tobacco column cap strong adhesion, adopt the tobacco seed seed oil medium pollen configured to carry out artificial pollination, pollination efficiency is high, effective, effectively can avoid waste of running off, and saves pollen consumption, and then effectively reduces breeding cost.
Embodiment
Below in conjunction with embodiment and Application Example to the present invention do technology contents and technique effect further in detail, but they are not limitation of the invention.
embodiment 1
By tobacco seed clean water 3 times, consider after solid carbon dioxide divides, be placed in drying box and be dried to seed moisture content lower than 5% in 35 DEG C, adopt physical squeezing method to squeeze out tobacco seed seed oil, after considering impurity with 60 mesh sieves, save backup under being placed in 4 ° of C.Take the pure pollen of cloud and mist 87, add in tobacco seed seed oil, be mixed with the tobacco seed seed oil medium pollen that pollen amount is 25mg/ml.With little writing brush by the tobacco seed seed oil medium pollen uniform application for preparing in maternal column cap, carry out hybrid seeding artificial pollination.
embodiment 2
Repeat embodiment 1, difference is: be mixed with the tobacco seed seed oil medium pollen that pollen amount is 40mg/ml.
embodiment 3
Repeat embodiment 1, difference is: be mixed with the tobacco seed seed oil medium pollen that pollen amount is 50mg/ml.
embodiment 4
Repeat embodiment 1, difference is: employing be the pure pollen of K326.
embodiment 5
Repeat embodiment 2, difference is: employing be the pure pollen of K326.
embodiment 6
Repeat embodiment 3, difference is: employing be the pure pollen of K326.
application Example 1
By embodiment 1 ~ 3, preparation pollen amount is the cloud and mist 87 tobacco seed seed oil medium pollen of 25mg/ml, 40mg/ml, 50mg/ml, and pollen amount is the cloud and mist 87 tobacco seed seed oil medium pollen of 10mg/ml, 75mg/ml, with the pure pollen of cloud and mist 87 for contrast.With little writing brush by the tobacco seed seed oil medium pollen for preparing and the pure pollen uniform application of cloud and mist 87 in MS cloud and mist 87 column cap, carry out hybrid seeding artificial pollination.Each process arranges 3 repetitions, and each repetition area is 1 mu.Every strain cigarette is pollinated 250 flowers, and pollination terminates latter 7 days statistics percentages of fertile fruit.The field capsule brown ripe later stage gathers, and after adopting same procedure selected, is dried by seed to water content and is less than 6%.Add up every mu of seed average yield, and carry out seed germination experiment under 25 ° of C, statistics germination vigor and germination rate.Result of the test is as table 1.
Table 1 tobacco seed seed oil medium pollen compares MS cloud and mist 87 Pollination Effect
Result of the test shows, adopt the tobacco seed seed oil medium pollen pollination of 25mg/ml, 40mg/ml, 50mg/ml, percentage of fertile fruit all reaches more than 90%, apparently higher than contrast; Seed production is respectively 17.0kg/ mu, 22.6kg/ mu, 22.5kg/ mu, all apparently higher than contrast; Potentiality of seed and germination rate are all a little more than contrast; The pollination of 100mg pollen spends number all significantly to improve.Illustrate, adopting the pollination of cloud and mist 87 tobacco seed seed oil medium pollen, when effectively reducing pollen consumption, improve percentage of fertile fruit and seed production, germination vigor, the germination rate of MS cloud and mist 87.And discovery when adopting tobacco seed seed oil medium pollen to carry out artificial pollination, tobacco seed seed oil toughness is high, and with tobacco column cap strong adhesion, pollination efficiency is high, effectively can avoid waste of running off, and saves recruitment cost.
Adopt the tobacco seed seed oil medium pollen pollination of 10mg/ml, although the pollination of 100mg pollen spends number significantly to improve, saved pollen consumption, reduced seed production.Adopt the tobacco seed seed oil medium pollen pollination of 75mg/ml, indices is all better than contrast, but processes ratio with 25mg/ml, 40mg/ml, 50mg/ml tri-, and Pollination Effect promotes not obvious.
application Example 2
Repeated application embodiment 1, has following difference: employing be K326 tobacco seed seed oil medium pollen and pure pollen, artificial pollination is carried out to MSK326.Result of the test is in table 2.
Table 2 tobacco seed seed oil medium pollen compares MSK326 Pollination Effect
Result shows, adopt the tobacco seed seed oil medium pollen pollination of 25mg/ml, 40mg/ml, 50mg/ml, percentage of fertile fruit is all apparently higher than contrast, and seed production is respectively 16.6kg/ mu, 19.8kg/ mu, 19.2kg/ mu, all apparently higher than contrast; Potentiality of seed and germination rate are all a little more than contrast; The pollination of 100mg pollen spends number all significantly to improve.Show the result similar to Application Example 1.
Illustrating, adopt the pollination of tobacco seed seed oil medium pollen, when effectively reducing pollen consumption, percentage of fertile fruit and seed production, germination vigor, germination rate can be improved.And adopt tobacco seed seed oil medium pollen to carry out artificial pollination, with tobacco column cap strong adhesion, pollination efficiency is high, effectively can avoid waste of running off, and saves recruitment cost.
Claims (3)
1. tobacco seed seed oil medium pollen, is characterized in that: contain tobacco pure pollen 25-50 milligram in every milliliter of tobacco seed seed oil in this tobacco seed seed oil medium pollen.
2. the preparation method of tobacco seed seed oil medium pollen as claimed in claim 1, is characterized in that: comprise the following steps,
A. tobacco seed clear water is cleaned, dry to water content lower than 5%, adopt physical squeezing method to squeeze out tobacco seed seed oil, after considering impurity with 60 mesh sieves, save backup under being placed in 4 ° of C;
B. take the pure pollen of tobacco, add in tobacco seed seed oil, add the amount of the pure pollen of 25-50 milligram tobacco according to every 1 milliliter of tobacco seed seed oil, Homogeneous phase mixing.
3. the purposes of tobacco seed seed oil medium pollen as claimed in claim 1, is characterized in that: purposes is applied on the maternal column cap of tobacco flower, carries out tobacco hybridization production of hybrid seeds artificial pollination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510565007.2A CN105123506A (en) | 2015-09-08 | 2015-09-08 | Tobacco seed oil medium pollen and preparation method and purpose thereof. |
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| CN201510565007.2A CN105123506A (en) | 2015-09-08 | 2015-09-08 | Tobacco seed oil medium pollen and preparation method and purpose thereof. |
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Citations (5)
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| CN101385437A (en) * | 2008-09-26 | 2009-03-18 | 玉溪中烟种子有限责任公司 | Solid medium pollen and preparation method and use thereof |
| CN101554133A (en) * | 2009-05-08 | 2009-10-14 | 云南省烟草农业科学研究院 | Liquid medium pollen, preparing method and application thereof |
| CN101946692A (en) * | 2010-08-13 | 2011-01-19 | 云南省烟草农业科学研究院 | Tobacco compound pollen medium, preparation method and applications thereof |
| CN102124942A (en) * | 2010-11-26 | 2011-07-20 | 云南省烟草农业科学研究院 | Tobacco pollen medium as well as preparation method and application thereof |
| CN103004753A (en) * | 2012-12-11 | 2013-04-03 | 浙江大学 | Long-time watermelon pollen preservation method and corresponding fruit setting promotion method by using early spring facility |
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2015
- 2015-09-08 CN CN201510565007.2A patent/CN105123506A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385437A (en) * | 2008-09-26 | 2009-03-18 | 玉溪中烟种子有限责任公司 | Solid medium pollen and preparation method and use thereof |
| CN101554133A (en) * | 2009-05-08 | 2009-10-14 | 云南省烟草农业科学研究院 | Liquid medium pollen, preparing method and application thereof |
| CN101946692A (en) * | 2010-08-13 | 2011-01-19 | 云南省烟草农业科学研究院 | Tobacco compound pollen medium, preparation method and applications thereof |
| CN102124942A (en) * | 2010-11-26 | 2011-07-20 | 云南省烟草农业科学研究院 | Tobacco pollen medium as well as preparation method and application thereof |
| CN103004753A (en) * | 2012-12-11 | 2013-04-03 | 浙江大学 | Long-time watermelon pollen preservation method and corresponding fruit setting promotion method by using early spring facility |
Non-Patent Citations (5)
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| 几种果树花粉有机溶剂保存的研究;刘国俭等;《河北林果研究》;19981231;第13卷(第4期);第339-342页 * |
| 刘国俭等: "几种果树花粉有机溶剂保存的研究", 《河北林果研究》 * |
| 张继澍: "《植物生理学》", 31 January 2006, 高等教育出版社 * |
| 液体介质花粉授粉对烟草坐果和种子活力及幼苗的影响;郑昀晔等;《浙江农业学报》;20111231;第23卷(第1期);第35-40页 * |
| 郑昀晔等: "液体介质花粉授粉对烟草坐果和种子活力及幼苗的影响", 《浙江农业学报》 * |
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Application publication date: 20151209 |