CN103190335B - Simple method for artificially synthesizing cabbage type rape - Google Patents
Simple method for artificially synthesizing cabbage type rape Download PDFInfo
- Publication number
- CN103190335B CN103190335B CN2013101175530A CN201310117553A CN103190335B CN 103190335 B CN103190335 B CN 103190335B CN 2013101175530 A CN2013101175530 A CN 2013101175530A CN 201310117553 A CN201310117553 A CN 201310117553A CN 103190335 B CN103190335 B CN 103190335B
- Authority
- CN
- China
- Prior art keywords
- type rape
- cabbage
- hours
- ovary
- artificially synthesized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a simple method for artificially synthesizing a cabbage type rape. The simple method comprises the following steps of: when wild cabbage and Chinese cabbage are hybridized, carrying out fertilization on the stigma of a female parent; after 16-144 hours, directly spraying a colchicine aqueous solution with percent concentration of 0.05%-0.15% on young ovary; and after the ovary grows to naturally-matured silique, harvesting full seeds, i.e., obtaining artificially-synthesized wild cabbage type rape seeds. Therefore, a new means with convenience, fastness and high efficiency is provided for obtaining the new germplasm of the cabbage type rape.
Description
Technical field
The present invention relates to field of crop genetic breeding, particularly a kind of method of artificially synthesized Brassica type rape.
Background technology
Artificially synthesized Brassica type rape is the effective way of widening genetics breeding of rape germ plasm resource, is also research rape species origin and one of the important means that develop.In the process of Synthetic rapeseed, because wild cabbage (CC=20) is not identical with incompatibility and the chromosome number of Chinese cabbage (AA=18) interspecific cross, heterozygote, heterozygote embryo are difficult to develop into normal embryo; Even obtained hybrid F1(AC=19) seed also can not educate, need to could obtain the cabbage type rape (AACC=38) of the allotetraploid that can educate by chromosome doubling.At present, in order to obtain artificially synthesized Brassica type rape, often take following methods: 1) directly extensive hybrid method, in wild cabbage and Chinese cabbage, make male parent with one, another of emasculation done maternal, once hybridizes 1000 and spends above, to obtaining the species hybrid cabbage type rape, the probability that this method obtains species hybrid is very low; 2) repeat the pollination method, or claim mentor method, take Chinese cabbage as male parent, wild cabbage is that female parent is example, successively pollinates twice, first on the chapiter of wild cabbage, awards the pollen with self, approximately every five hours, award again the pollen with Chinese cabbage, once must be species hybrid in this way later; 3) the rescue method of ovary or embryo culture, pollinate latter about 15 days, get ovary or embryo is cultivated, then the monoploid hybrid seedling is being processed about 10 days containing preculture in the MS medium of 0.01% colchicin, carry out chromosome doubling, finally obtain artificial synthetic cabbage type rape.
By these methods, although obtained some artificial Synthetic rapeseed, all there are the following problems for the method for acquisition artificially synthesized Brassica type rape at present: workload is large, length consuming time, and complexity, efficiency is low.The hereditary basis of the main oil crop cabbage type rape in the horn of plenty world, lay good basis for obtaining higher cabbage type rape heterosis utilization, simple to operate in the urgent need to developing a kind of energy, workload is little, the new method of the acquisition artificially synthesized Brassica type rape that efficiency is high.
Summary of the invention
Technical problem to be solved by this invention is: incompatibility and workload for wild cabbage in prior art and Chinese cabbage interspecific cross are large, length consuming time, complexity, inefficient problem, provide a kind of simple to operate, workload is little, the method for the artificially synthesized Brassica type rape that efficiency is high.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of method of artificially synthesized Brassica type rape, it is by wild cabbage and Chinese cabbage hybridization the time, in maternal column cap, pollinate between latter 16 hours-144 hours, directly to the children, tender ovary sprays the colchicine solution that mass percent concentration is 0.05%-0.15%, after the angle fruit that becomes physical maturity until ovary development, full seed in the fruit of results angle, obtain.
One preferred embodiment of such scheme is, latter 16 hours of pollination directly to the children tender ovary to spray mass percent concentration be 0.15% colchicine solution 1 time.
One preferred embodiment of such scheme is, latter 24 hours of pollination directly to the children tender ovary to spray mass percent concentration be 0.1% colchicine solution 1 time, every 24 hours, spray 1 time afterwards, spray altogether 6 times.
One preferred embodiment of such scheme is, at pollination 24 hours and 48 hours two time points, directly to the children, tender ovary sprays respectively the colchicine solution that mass percent concentration is 0.05% and 0.1%.
The described female parent of such scheme is wild cabbage or Chinese cabbage.
The Chinese cabbage of mentioning herein is turnip type rape.
Compared with prior art, the beneficial effect that the present invention has is: in the appropriate time of the present invention after wild cabbage and Chinese cabbage hybridization pollination, the tender ovary of children after pollination is directly sprayed to colchicine solution, the incompatibility and the workload that have so not only overcome the wild cabbage that exists in the prior art and Chinese cabbage interspecific cross are large, length consuming time, complexity, inefficient problem, and the present invention is for artificially synthesized Brassica type rape, simple to operate, workload is little, and efficiency is high.
The accompanying drawing explanation
The PCR of the artificially synthesized Brassica type rape that Fig. 1 is wild cabbage G3 * Chinese cabbage B1 acquisition detects figure.
The PCR of the artificially synthesized Brassica type rape that Fig. 2 is wild cabbage G2 * Chinese cabbage B2-2 acquisition detects figure.
The PCR of the artificially synthesized Brassica type rape that Fig. 3 is wild cabbage G4 * Chinese cabbage B1 acquisition detects figure.
Embodiment
Embodiment 1: utilize wild cabbage G3 strain and Chinese cabbage B1 strain artificially synthesized Brassica type rape
1. material plantation: on October 10th, 2010, wild cabbage G3 strain and Chinese cabbage B1 strain are planted in the rape experimental field, on March 10th, 2011, Chinese cabbage B1 enters flowering stage, and March 23, rigan blue G 3 entered flowering stage.
2. hybridization: the Chinese cabbage B1 of blooming morning of take is male parent, and the relatively wild cabbage G3 in evening of blooming is female parent.Between morning 9-10 point, on the inflorescence of wild cabbage G3, choose that petal will launch but 11, the bud do not opened, other bud of opening and not opening all removes; Then thoroughly remove the stamen of 11 flowers, the pollen of Chinese cabbage B1 is awarded on the column cap of wild cabbage G3, with sulfuric acid paper bag sack bagging, in case the pollen in other source is fallen on column cap.
3. colchicine is processed: after pollinating 16 hours, remove sack, the tender ovary of the children of 11 flowers is sprayed to the colchicine solution that mass percent concentration is 0.15%.Wait afterwards ovary development to become rear sowing of angle fruit of physical maturity, shrivelled seed and full seed are wherein arranged.
4. the detection of artificially synthesized Brassica type rape: 1 full planting seed that will obtain is in the nutritive cube with native, after seed germination has grown into three true leaves, extract the total DNA in blade, utilization can be distinguished wild cabbage, the PCR method of Chinese cabbage and cabbage type rape is identified, the pcr amplification result shows, only amplify a less band from wild cabbage G3, only amplify a larger band from Chinese cabbage B1, filial generation size 2 bands all increase out (Fig. 1), illustrate after hybridization that what obtain is real artificial synthetic cabbage type rape, consequently obtain the artificial synthetic cabbage type rape GB-0 of 1 strain.
Embodiment 2: utilize wild cabbage G2 strain and Chinese cabbage B2-2 strain artificially synthesized Brassica type rape
The material plantation: on October 10th, 2011, wild cabbage G2 strain and Chinese cabbage B2-2 strain are planted in the rape experimental field, on March 10th, 2012, Chinese cabbage B2-2 enters flowering stage, and March 23, G2 entered flowering stage.
Hybridization: the Chinese cabbage B2-2 of blooming morning of take is male parent, and the relatively wild cabbage G2 in evening of blooming is female parent.Between morning 9-10 point, on the inflorescence of wild cabbage G2, choose that petal will launch but 18, the bud do not opened, other bud of opening and not opening all removes; Then thoroughly remove the stamen of 18 flowers, the pollen of Chinese cabbage B2-2 is awarded on the column cap of wild cabbage G2, with sulfuric acid paper bag sack bagging, in case the pollen in other source is fallen on column cap.
Colchicine is processed: after pollinating 24 hours, remove sack, the tender ovary of the children of 18 flowers is sprayed to the colchicine solution that mass percent concentration is 0.1%.Sprayed once every 24 hours afterwards, spray altogether 6 times, wait ovary development to become rear sowing of angle fruit of physical maturity, shrivelled seed and full seed are wherein arranged.
The detection of artificially synthesized Brassica type rape: 2 full planting seeds that will obtain are in the nutritive cube with native, after seed germination has grown into three true leaves, extract the total DNA in blade, utilization can be distinguished wild cabbage, the PCR method of Chinese cabbage and cabbage type rape is identified, the pcr amplification result shows, only amplify a less band from wild cabbage G2, only amplify a larger band from Chinese cabbage B2-2, filial generation size 2 bands all increase out (Fig. 2), illustrate after hybridization that what obtain is real artificial synthetic cabbage type rape, its result obtains artificial synthetic cabbage type rape GB-1 and the GB-2 of 2 strains.
Embodiment 3: utilize wild cabbage G4 strain and Chinese cabbage B1 strain artificially synthesized Brassica type rape
The material plantation: on October 10th, 2011, wild cabbage G4 strain and Chinese cabbage B1 strain are planted in the rape experimental field, on March 10th, 2012, Chinese cabbage B1 enters flowering stage, and March 23, G4 entered flowering stage.
Hybridization: the Chinese cabbage B1 of blooming morning of take is male parent, and the relatively wild cabbage G4 in evening of blooming is female parent.Between morning 9-10 point, on the inflorescence of wild cabbage G4, choose that petal will launch but 23, the bud do not opened, other bud of opening and not opening all removes; Then thoroughly remove the stamen of 23 flowers, the pollen of Chinese cabbage B1 is awarded on the column cap of wild cabbage G4, with sulfuric acid paper bag sack bagging, in case the pollen in other source is fallen on column cap.
Colchicine is processed: after pollinating 24 hours, remove sack, the tender ovary of the children of 23 flowers is sprayed to the colchicine solution that mass percent concentration is 0.05%, the colchicine solution that the mass percent concentration of filling spray again after pollinating 48 hours is 0.1%.Allow afterwards ovary development become rear sowing of angle fruit of physical maturity, shrivelled seed and full seed are wherein arranged.
The detection of artificially synthesized Brassica type rape: 3 full planting seeds that will obtain are in the nutritive cube with native, after seed germination has grown into three true leaves, extract the total DNA in blade, utilization can be distinguished wild cabbage, the PCR method of Chinese cabbage and cabbage type rape is identified, the pcr amplification result shows, only amplify a less band from wild cabbage G4, only amplify a larger band from Chinese cabbage B1, filial generation size 2 bands all increase out (as shown in Figure 3), illustrate after hybridization that what obtain is real artificial synthetic cabbage type rape, its result obtains the artificial synthetic cabbage type rape GB4 of 3 strains, GB5 and GB6.
Claims (4)
1. the method for an artificially synthesized Brassica type rape, it is characterized in that: the method is by wild cabbage and Chinese cabbage hybridization the time, in maternal column cap, pollinate latter 16 hours-144 hours, directly to the children, tender ovary sprays the colchicine solution that mass percent concentration is 0.05%-0.15%, after the angle fruit that becomes physical maturity until ovary development, full seed in the fruit of results angle, obtain.
2. the method for artificially synthesized Brassica type rape as claimed in claim 1 is characterized in that: latter 16 hours of pollination directly to the children tender ovary to spray mass percent concentration be 0.15% colchicine solution 1 time.
3. the method for artificially synthesized Brassica type rape as claimed in claim 1, it is characterized in that: latter 24 hours of pollination directly to the children tender ovary to spray mass percent concentration be 0.1% colchicine solution 1 time, sprayed 1 time every 24 hours afterwards, spray altogether 6 times.
4. the method for artificially synthesized Brassica type rape as claimed in claim 1 is characterized in that: at pollination 24 hours and 48 hours two time points, directly to the children, tender ovary sprays respectively the colchicine solution that mass percent concentration is 0.05% and 0.1%.5, the method for artificially synthesized Brassica type rape as claimed in claim 1 or 2 or 3 or 4, it is characterized in that: described female parent is wild cabbage or Chinese cabbage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101175530A CN103190335B (en) | 2013-04-07 | 2013-04-07 | Simple method for artificially synthesizing cabbage type rape |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013101175530A CN103190335B (en) | 2013-04-07 | 2013-04-07 | Simple method for artificially synthesizing cabbage type rape |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103190335A CN103190335A (en) | 2013-07-10 |
| CN103190335B true CN103190335B (en) | 2013-12-11 |
Family
ID=48713304
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013101175530A Active CN103190335B (en) | 2013-04-07 | 2013-04-07 | Simple method for artificially synthesizing cabbage type rape |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103190335B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718824B (en) * | 2016-11-16 | 2019-04-23 | 西南大学 | The method for preparing high erucic acid cabbage type rape using artificial synthesis Brassica napus |
| CN106718823B (en) * | 2016-11-16 | 2019-04-23 | 西南大学 | A method of high oleic acid rape material is prepared using artificial synthesis Brassica napus |
| CN109042291A (en) * | 2018-07-28 | 2018-12-21 | 湖南科技大学 | A method of novel vegetable is created using Chinese cabbage and wild cabbage distant hybridization |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102301946B (en) * | 2011-07-26 | 2012-12-26 | 江苏省农业科学院 | Method for creating yellow-seeded brassica napus germplasm |
-
2013
- 2013-04-07 CN CN2013101175530A patent/CN103190335B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102301946B (en) * | 2011-07-26 | 2012-12-26 | 江苏省农业科学院 | Method for creating yellow-seeded brassica napus germplasm |
Non-Patent Citations (1)
| Title |
|---|
| 陆莉.一种快速生长的新型甘蓝型油菜的合成及其小孢子培养的研究.《扬州大学硕士学位论文》.2008, * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103190335A (en) | 2013-07-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101422133B (en) | Cabbage type rape self-incompatibility two-line hybrid seed-selection method | |
| AU2016410430B2 (en) | Method for breeding cruciferous vegetable materials and varieties with double haploid induction line of rapeseed | |
| CN104429929B (en) | Maninot esculenta crantz. hermaphrodite flower breeding method | |
| CN103081797B (en) | Method for inducing corn haploid | |
| CN106069718B (en) | The method of rape dihaploid induction system selection and breeding cabbage type rape cytoplasmic male sterile line | |
| CN105104168B (en) | The close hybrid rice breeding method of parent's duration from seeding to heading | |
| CN105052724A (en) | Method for improving self-pollination seed setting percentage of diploid potatoes and application of method | |
| CN103416296B (en) | A kind of method improving water lily self-fruitful rate | |
| CN109349099A (en) | Brassica napus recessive gms line is close, distant hybridization selectively breeding hybrid rape selection | |
| CN103190335B (en) | Simple method for artificially synthesizing cabbage type rape | |
| CN103548672A (en) | Breeding, reproduction and seed production method for cytoplasmic male sterile line of Chinese flowering cabbage | |
| CN105075853A (en) | Breeding method of sightseeing white flower brassica napus | |
| CN104429918A (en) | Method for improving brassica napus type temporary maintainer line of nuclear recessive epistatic interaction sterility in rapes | |
| Yu et al. | Characterization of a new temperature-sensitive male sterile line SP2S in rapeseed (Brassica napus L.) | |
| CN104521740B (en) | Synchronous transform-breeding method for wuta-cai homozygous inbred line and cytoplasmic male sterile line | |
| CN105724244A (en) | Method for conducting cross breeding through homozygous parent prepared through selfing of bisexual plant and supermale | |
| CN103392606A (en) | Cultivation method of polyploid pomegranate | |
| CN103190336A (en) | Method for transforming wild cabbage into male sterility line of kohlrabi and breeding hybrids | |
| CN107660462B (en) | Method for creating hot pepper cytoplasmic male sterile line and simultaneously matching three lines | |
| CN109691387B (en) | Breeding method of male sterile cabbage type rape hybrid | |
| CN107410015A (en) | A kind of selection of leafy mustard cytoplasmic male sterile line | |
| CN107410011A (en) | A kind of method of efficiently quick separating cabbage type rape A and C subgenome | |
| CN108935068B (en) | Method for improving fertility temperature sensitivity of brassica napus pol CMS sterile line | |
| CN107484655B (en) | A kind of selection of anti-clubroot preserved sichuan pickle cytoplasm male sterility hybrid | |
| CN102919116B (en) | Selection breeding method of new homozygous sterile line of brassica napus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |