CN105028175A - Breeding method of selfing lines of waxy corns and white waxy corns - Google Patents
Breeding method of selfing lines of waxy corns and white waxy corns Download PDFInfo
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Abstract
The invention discloses a breeding method of selfing lines of waxy corns and white waxy corns. A breeding method of the selfing line of the waxy corns comprise the following steps: hybridizing by taking one of a plurality of waxy corn species as a female parent, and the other waxy corn species as a male parent, so as to form a plurality of hybridized combinations; selecting one of the hybridized combinations, collecting pollen of the other hybridized combinations, and uniformly mixing at equal amount; pollinating the selected hybridized combination by using the uniformly mixed pollen, and thus obtaining a target hybrid seed of the pollen and the selected hybridized combinations; and carrying out selfing breeding on the target hybrid seed according to the preset target characters, and thus obtaining a stable waxy corn selfing line. In the breeding process, by increasing the step of selecting and remaining white grains, the breeding of the selfing line of the white waxy corns can be realized. Through hybridization and pollination by adopting the mixed pollen, excellent gene resources can be collected, so that the bred selfing line can adapt to various ecological forms, and has outstanding high temperature resistance and low temperature resistance, and strong disease resistance and stress resistance.
Description
Technical field
The present invention relates to plant breeding technique field, particularly relate to the selection of a kind of waxy corn and white Waxy Corn Inbred Lines.
Background technology
Along with the raising of living standards of the people and the reinforcement of health care consciousness, people propose new requirement to the kind of Waxy Maize product, nutrition, the aspect such as appearance luster and health care.Fresh, good to eat waxy corn product is due to features such as its unique flavor, nutritious, health leisure, and dark liking by consumers in general, add that suitable store and processing can year-round supply, the market demand constantly expands.
In addition, because Waxy Maize breeding time is shorter, cultivation relative ease, remarkable in economical benefits, its area under cultivation is constantly expanded.
But changeable along with weather in recent years, adds new demand to Glutinous Corn Varieties, not only require best in quality, output is high, more needs the feature possessing wide adaptability, strong stress resistance.
For this reason, cultivate can be mixed with high-quality, high yield, good stress resistance, wide adaptability the demand of inbred line of Waxy Maize crossbreed more and more stronger.But the waxy corn landrace of existing eurytopicity, high-quality, high yield is deficienter, the glutinous corn variety that selection selects is difficult to meet the need of market.
Therefore, prior art awaits development.
Summary of the invention
In view of above-mentioned the deficiencies in the prior art part, the object of the present invention is to provide the selection of a kind of waxy corn and white Waxy Corn Inbred Lines, the glutinous corn variety being intended to solve the acquisition of existing glutinous corn variety selection is difficult to the problem of meeting the need of market.
In order to achieve the above object, this invention takes following technical scheme:
A kind of Waxy Corn Inbred Lines selection, wherein, comprises the steps:
Select some glutinous corn varieties, be maternal by one of them glutinous corn variety in some glutinous corn varieties, other glutinous corn varieties are that male parent is hybridized, and form multiple hybrid combination;
Select one of them hybrid combination in described multiple hybrid combination, collect other hybrid combinations except the hybrid combination chosen pollen and by even for the pollen mixed in equal amounts of each hybrid combination;
The pollen mixed described in using is pollinated to the hybrid combination chosen, and obtains pollen and the target hybrid seed of hybrid combination chosen;
According to the objective trait preset, selfed breeding is carried out to described target hybrid seed, obtains stable Waxy Corn Inbred Lines.
Described Waxy Corn Inbred Lines selection, wherein, described some glutinous corn varieties comprise: Su Yu is glutinous 901, it is glutinous 930 to change, osmanthus is glutinous 519, Su Yu glutinous 639 and beautiful agriculture crystalline substance glutinous; Select glutinous for Su Yu 901 as maternal.
Described Waxy Corn Inbred Lines selection, wherein, described in choose be combined as Su Yu glutinous 901 and Chongqing glutinous 930.
Described Waxy Corn Inbred Lines selection, wherein, the step of described collection pollen of other hybrid combinations except the hybrid combination chosen is specially: respectively the seed of 4 hybrid combinations is respectively planted 5 strains, after forming plant, obtains the some grams of pollen of each hybrid combination plant.
Described Waxy Corn Inbred Lines selection, wherein, described some grams of pollen are specially 5g.
Described Waxy Corn Inbred Lines selection, wherein, described default objective trait comprises: head progeny row and plant resistance, fruit ear commodity.
Described Waxy Corn Inbred Lines selection, wherein, described selfed breeding method specifically comprises the steps: described target hybrid seed to plant some strains, bagging selfing after formation plant; And neat kernel arrangement of selecting and remain after fruit ear results, tassel row number are the fruit ear of 14 row and full seed, a point single fringe threshing obtains single fringe seed; Divide head progeny row to plant in the single fringe seed obtained, according to the objective trait preset, select corresponding plant selfing 5-6 generation, obtain stable Waxy Corn Inbred Lines.
Described Waxy Corn Inbred Lines selection, wherein, described method also comprises: divide head progeny row to plant in the single fringe seed obtaining stable Waxy Corn Inbred Lines, each head progeny row plants some strains, select the selfing of fine individual plant bagging, according to inbred line conventional herd breeding method continuous selfing 5 ~ 6 generation.
A kind of selection applying the white Waxy Corn Inbred Lines of inbred line breeding method seed selection as above, wherein, the selection of described white Waxy Corn Inbred Lines is selected and remain white-kernel respectively after the step of the step of above-mentioned " forming multiple hybrid combination " and " obtaining pollen and the target hybrid seed of hybrid combination chosen ".
Beneficial effect: the selection of a kind of waxy corn provided by the invention and white Waxy Corn Inbred Lines, selects the excellent glutinous corn variety of performance to hybridize, then mixes getting equivalent pollen respectively after hybrid combination plantation.Then use mixed pollen to pollinate, thus the excellence gene of separate sources resource are flocked together, provide the abundanter waxy corn assortment of genes for seed selection.The method of last conveniently seed selection is again bred as stable inbred line, and preparation possesses the excellent New Waxy Maize Hybrids of eurytopicity, high-quality, high yield characteristics, effectively meets the need of market.
Accompanying drawing explanation
Fig. 1 is the method flow diagram of the Waxy Corn Inbred Lines selection of the specific embodiment of the invention.
Embodiment
The invention provides the selection of a kind of waxy corn and white Waxy Corn Inbred Lines.For making object of the present invention, technical scheme and effect clearly, clearly, developing simultaneously referring to accompanying drawing, the present invention is described in more detail for embodiment.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
As shown in Figure 1, be a kind of Waxy Corn Inbred Lines selection of the specific embodiment of the invention.Described selection comprises the steps:
S1, select some glutinous corn varieties, be maternal by one of them glutinous corn variety in some glutinous corn varieties, other glutinous corn varieties are that male parent is hybridized, and form multiple hybrid combination.
Described some glutinous corn varieties can be any suitable Good Quality Glutinous Corn kind or strain, also can the glutinous corn variety suitable according to the selection that actual demand is purposive and quantity.In the present invention's specific embodiment, can glutinous 519, the Su Yu glutinous 639 in glutinous 901, glutinous 930, the osmanthus of changing of choice for use Su Yu and beautiful agriculture crystalline substance glutinous, select glutinous for Su Yu 901 as female parent.Above-mentioned glutinous corn variety is existing general types, and the parameters such as concrete proterties are well known to those skilled in the art, and therefore not to repeat here.
Material based on the excellent glutinous corn variety of above-mentioned selection different geographical, by the mode that following hybridization and pollen mixture are pollinated, favorable genes resource can be assembled, cause the inbred line of incubation to adapt to multiple ecotype, high temperature resistant and resistance to low temperature is given prominence to, and disease-resistant resistance is stronger.
The plant that the hybrid combination of described formation is formed after specifically can referring to the seed that formed by two inter breed crossings or the plantation of this seed or possess the genotypic life entity that these two inter breed crossings are formed.Such as, described hybrid combination can be leaf, fruit or waxy corn plant etc.
S2, one of them hybrid combination selected in described multiple hybrid combination, collect other hybrid combinations except the hybrid combination chosen pollen and by even for the pollen mixed in equal amounts of each hybrid combination.
The selection of concrete hybrid combination can be selected according to specific situation, and the hybrid combination chosen should have more excellence (or target) proterties, because its genotype ratio in the gene pool of follow-up formation is higher.In one particular embodiment of the present invention, the hybrid combination of selection is Su Yu glutinous 901 and Chongqing glutinous 930.
S3, use described in the pollen that mixes the hybrid combination chosen is pollinated, obtain the target hybrid seed of pollen and the hybrid combination chosen.
Obtain the seed of multiple hybrid combination in step S1 after, need to be planted formation plant.Choose one of them in multiple hybrid combination as Pollon receiver (female parent).Obtain other hybrid combination plant pollen and by its mixed in equal amounts evenly after the plant of the hybrid combination chosen is pollinated.Choose hybrid combination plant quantity and the pollen quantity of concrete use can be determined according to actual needs.Such as, when having planted more hybrid combination plant, need the pollen quantity of acquisition then more.The method of concrete pollen mixing can use existing conventional mixed method, such as, stir etc.
The mode of hybridizing is carried out with choosing hybrid combination again after adopting the pollen of each hybrid combination of above-mentioned mixing, the excellence gene of separate sources (i.e. the glutinous corn variety of above-mentioned use) can be flocked together, for follow-up selfed breeding provides a huge gene pool, the genetic integration of multiple excellence can be realized, there is the possibility of the glutinous corn variety selecting more greatly suitable trait.
The objective trait that S4, foundation are preset, carries out selfed breeding to described target hybrid seed, obtains stable Waxy Corn Inbred Lines.
After obtaining target hybrid seed (being formed by pollen mixture and the hybrid combination chosen), plantation is its concrete proterties of observable after forming plant.The method of selfed breeding can be adopted to obtain finally stable Waxy Corn Inbred Lines.Concrete selfed breeding method can use the selfed breeding method of existing routine, also can be for further adjustments to the selfed breeding method of routine according to specific needs.
Described objective trait refers to the concrete character requirement of target seed selection plant, turgor of such as tassel row number, plant resistance, fruit ear etc.In the present invention's specific embodiment, described default objective trait specifically comprises: the commodity of tassel row number, plant resistance, fruit ear.Wherein, the commodity of fruit ear is well known to those skilled in the art, and refer to the concrete proterties such as the exterior of commodity affecting fruit ear, the neat degree of such as fruit ear, whether full, whether size is consistent.
It should be noted that the sequence number before above-mentioned steps only in order to represent the logical relation between step, and be not used in the operating sequence limited between step.Those skilled in the art can exchange the order of above-mentioned steps or increase/other suitable steps of economization in practical operation.
Concrete, the method for described selfed breeding specifically comprises:
First, described target hybrid seed is planted some strains, bagging selfing after formation plant; And neat kernel arrangement of selecting and remain after fruit ear results, tassel row number are the fruit ear of 14 row and full seed, a point single fringe threshing obtains single fringe seed.
Then, by the single fringe seed obtained divide head progeny row to plant, according to the objective trait preset, select corresponding plant selfing 5-6 generation, obtain stable Waxy Corn Inbred Lines.
It is wherein, described that described target hybrid seed is planted some strains is 20 strains.In conjunction with some glutinous corn varieties that above-mentioned specific embodiment is selected, final stable Waxy Corn Inbred Lines wide adaptability, the strong stress resistance obtained.Economical character is excellent, and plant strain growth stalwartness, graceful, the middle upper punch of plant type, leaf look dark green, bract consolidation, suitable dense planting.And fruit ear commodity is good, quality is good, the pure white look of fruit ear seed, head progeny row marshalling, full seed, fruit ear are well-balanced, nutritious, mouthfeel is unique, and pericarp is thin, by force waxy.
Certainly, this stable Waxy Corn Inbred Lines as outstanding basic material, can carry out further seed selection or the glutinous corn variety needed for preparation acquisition.Such as, further the Waxy Corn Inbred Lines of aforementioned stable and purplish red Waxy Corn Inbred Lines can be prepared the Hybrid grain look obtained red and white, not fade after boiling, red white clearly demarcated, the demand meeting market that can be good.
It is preferred that described selection can also comprise: divide head progeny row to plant in the single fringe seed obtaining stable Waxy Corn Inbred Lines, each head progeny row plants some strains, select the selfing of fine individual plant bagging, according to inbred line conventional herd breeding method continuous selfing 5 ~ 6 generation.More outstanding Waxy Corn Inbred Lines can be selected further by the method for above-mentioned further inbred line breeding.Wherein, each head progeny row is planted some strains and is specially 15 strains.
Present invention also offers a kind of method applying the white Waxy Corn Inbred Lines of above-mentioned selection seed selection.The method of the white waxy corn of described seed selection is specially respectively in " obtaining the target hybrid seed of pollen and the hybrid combination chosen " white-kernel of only selecting and remain afterwards of step S1 " forming multiple hybrid combination " and step S2.White-kernel of selecting and remain can ensure in follow-up waxy corn selfed breeding process, is white waxy corn thus realizes the seed selection of white Waxy Corn Inbred Lines.
A specific embodiment of selection of the present invention, described in specific as follows:
First, in March, 2010 ~ June, plant 10 strain Su Yu glutinous 901 in Nanjing, glutinous 930, the osmanthus of changing be glutinous 519, Su Yu glutinous 639 and brilliant glutinous each 5 strains of beautiful agriculture.Be maternal with Su Yu glutinous 901,, Su Yu glutinous 639 glutinous 519 with glutinous 930, the osmanthus of changing and beautiful agriculture is brilliant glutinously hybridizes 2 fringes respectively for male parent respectively, obtain the glutinous 901X of Su Yu and change glutinous 930, Su Yu glutinous 901X osmanthus glutinous 519, the seed of brilliant glutinous 4 hybrid combinations of the beautiful agriculture of Su Yu glutinous 901X the Su Yu glutinous 639 and glutinous 901X of Su Yu, separately threshing, white-kernel of selecting and remain.
Secondly, in July, 2010 ~ September, 4 each 5 strains of hybrid combination are planted in Nanjing, Post flowering gets Su Yu glutinous 901X osmanthus glutinous 519 respectively, the brilliant glutinous pollen 5 grams of the beautiful agriculture of Su Yu glutinous 901X the Su Yu glutinous 639 and glutinous 901X of Su Yu, combination Su Yu glutinous 901X glutinous 930 hybridization pollination 2 ~ 3 strains in Chongqing are given, white-kernel of selecting and remain in the target hybrid seed of results after mixing.
Again, in October, 2010 ~ 2011 year February, target seed above-mentioned steps obtained at Sanya, Hainan plants 20 strains, whole bagging selfing, 2 ~ 3, the fruit ear of neat kernel arrangement of selecting and remain after seed maturity, tassel row number 14 row, full seed, point single fringe threshing.
Finally, in March, 2011 ~ 2013 year February, in Nanjing and the Sanya, Hainan method according to corn inbred line conventional herd breeding, and select fine individual plant continuous selfing 5 ~ 6 generation in conjunction with plant resistance, fruit ear commodity etc. in head progeny row, be bred as stable white Waxy Corn Inbred Lines (the white Waxy Corn Inbred Lines QN-51 of called after) in February, 2013.And by selfing or the mixed method breeding original seed handed over.
The feature of the above-mentioned white Waxy Corn Inbred Lines QN-51 selected is specially: to sow in spring the time of infertility in In Nanjing 88 days, autumn sowing about 71 days.Seedling leaf sheath aubergine, dark green leaf color, cane is green, and aerial root is flourishing, capacity for the resistance to lodging is strong.Plant height 156 centimetres, Ear height 51 centimetres, total number of sheets 17 ~ 18, fringe upper blade number is 5, tassel branch number 15-18 is individual, pollen amount is large, the green white of filigree, fruit ear cartridge type, spike length 16 ~ 17 centimetres, tassel row number 14 ~ 16 row, several 32 of average row grain, grain look pure white look, thousand fresh weights 329 grams, dry weight 228 grams.Variable rate technology: the diseases such as the Chinese People's Anti-Japanese Military and Political College, helminthosporium maydis, head smut, banded sclerotial blight.
A kind of selection applying the white Waxy Corn Inbred Lines of inbred line breeding method seed selection as above, wherein, the selection of described white Waxy Corn Inbred Lines is selected and remain white-kernel respectively after the step of the step of above-mentioned " forming multiple hybrid combination " and " obtaining pollen and the target hybrid seed of hybrid combination chosen ".
Be understandable that, for those of ordinary skills, can be equal to according to technical scheme of the present invention and the present invention's design and replace or change, and all these change or replace the protection domain that all should belong to the claim appended by the present invention.
Claims (9)
1. a selection for Waxy Corn Inbred Lines, is characterized in that, comprises the steps:
Select some glutinous corn varieties, be maternal by one of them glutinous corn variety in some glutinous corn varieties, other glutinous corn varieties are that male parent is hybridized, and form multiple hybrid combination;
Select one of them hybrid combination in described multiple hybrid combination, collect other hybrid combinations except the hybrid combination chosen pollen and by even for the pollen mixed in equal amounts of each hybrid combination;
The pollen mixed described in using is pollinated to the hybrid combination chosen, and obtains pollen and the target hybrid seed of hybrid combination chosen;
According to the objective trait preset, selfed breeding is carried out to described target hybrid seed, obtains stable Waxy Corn Inbred Lines.
2. Waxy Corn Inbred Lines selection according to claim 1, is characterized in that, described some glutinous corn varieties comprise: Su Yu is glutinous 901, it is glutinous 930 to change, osmanthus is glutinous 519, Su Yu glutinous 639 and beautiful agriculture crystalline substance glutinous; Select glutinous for Su Yu 901 as maternal.
3. Waxy Corn Inbred Lines selection according to claim 2, is characterized in that, described in choose be combined as Su Yu glutinous 901 and Chongqing glutinous 930.
4. Waxy Corn Inbred Lines selection according to claim 2, it is characterized in that, the step of described collection pollen of other hybrid combinations except the hybrid combination chosen is specially: respectively the seed of 4 hybrid combinations is respectively planted 5 strains, after forming plant, obtain the some grams of pollen of each hybrid combination plant.
5. Waxy Corn Inbred Lines selection according to claim 4, is characterized in that, described some grams of pollen are specially 5g.
6. Waxy Corn Inbred Lines selection according to claim 1, is characterized in that, described default objective trait comprises: head progeny row and plant resistance, fruit ear commodity.
7. Waxy Corn Inbred Lines selection according to claim 1, is characterized in that, described selfed breeding method specifically comprises the steps:
Described target hybrid seed is planted some strains, bagging selfing after formation plant; And neat kernel arrangement of selecting and remain after fruit ear results, tassel row number are the fruit ear of 14 row and full seed, a point single fringe threshing obtains single fringe seed; Divide head progeny row to plant in the single fringe seed obtained, according to the objective trait preset, select corresponding plant selfing 5-6 generation, obtain stable Waxy Corn Inbred Lines.
8. Waxy Corn Inbred Lines selection according to claim 1, it is characterized in that, described method also comprises: divide head progeny row to plant in the single fringe seed obtaining stable Waxy Corn Inbred Lines, each head progeny row plants some strains, select the selfing of fine individual plant bagging, according to inbred line conventional herd breeding method continuous selfing 5 ~ 6 generation.
9. an application as arbitrary in claim 1-8 as described in the selection of the white Waxy Corn Inbred Lines of inbred line breeding method seed selection, it is characterized in that, white-kernel of selecting and remain after the step of " forming multiple hybrid combination " and the step of " obtaining pollen and the target hybrid seed of hybrid combination chosen " respectively.
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