CN108260524A - A kind of selection of radish cell nucleo-cytoplasmic interreaction male sterility maintainer line - Google Patents
A kind of selection of radish cell nucleo-cytoplasmic interreaction male sterility maintainer line Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention discloses a kind of selections of radish cell nucleo-cytoplasmic interreaction male sterility maintainer line, utilize the maintainer dog head tank B of Ogura cytoplasmic male sterility system dog head tank, the white B in the fertile plant stone village in the white dual purpose lines in the emasculation Hou Yushi village is hybridized, and obtains first-filial generation F1Group；Using the white A in the sterile plant stone village in the white dual purpose lines in the stone village to be maternal, the first-filial generation F1Group carries out test cross, while first-filial generation F for male parent1Group is selfed, observe test cross offspring's fertility performance's situation, screen the combination of offspring's infertility test cross, the male parent self progeny of offspring's infertility test cross combination is continued to be returned with the white B in the stone village, a new holding through-stone dog B is obtained after multiple test cross, backcrossing, the cytoplasm for keeping through-stone dog B contains the holding gene from dog head tank, and character and the Yuan Shi village are similar in vain.
Description
Technical field
The present invention relates to technical field of crop propagation, more particularly to a kind of radish cell nucleo-cytoplasmic interreaction male is not
Educate the selection of maintainer.
Background technology
Crucifer is mostly allogamy crop, there is apparent hybrid vigour, but due to their floral organ
Smaller, artificial emasculation operation is time-consuming and laborious, and the hybrid seed that primary pollination obtains is seldom, to solve this problem, breeding work
Author selects self incompatible line breeding, but self incompatible line is easily influenced by factors such as environment, and compatibility is unstable, often
There is the phenomenon that seed production purity is not high.Hybrid seeding is carried out using male sterility line of plants, it is not affine preferably to solve selfing
The problem of being the production of hybrid seeds, and the purity of hybrid seed can be improved, increase the yield of crops, oneself becomes the main development of international seed industry
Direction.
The radish male sterile used in production at present, it is typically jointly mutual by cytogene and cell nucleus gene
Make, a kind of male sterility phenomenon of formation, the sterile cytoplasm of radish generally has Ogura-CMS (orf138), Kos-CMS
(orf125), the types such as DCGMS-CMS and NWB-CMS, but the sterile types of most common or Ogura；By chondriogen
Two pairs of recessive genes control in orf138 and nucleus, sterile line genotype is Sms1ms1ms2ms2, and maintainer genotype is
Nms1ms1ms2ms2, (S is sterile type cytoplasm, and N is normal cell matter).Radish male sterility shows as Anther degeneration or not
The pollen with normal function can be formed, and Stamen development is normal.Maintainer then has normal male and female organ, selfing
Can be normal solid, when hybridizing with sterile line, the sterility of sterile line can be kept.With the continuous development of biotechnology, section
Radish Ogura male sterility genes are successively introduced into a variety of Cruciferae such as rape, wild cabbage, Chinese cabbage, cauliflower by skilled worker author
On crop.The radish male sterile that China has now been found that and uses is similarly Ogura cytoplasmic male sterilities, at present the whole world
In the range of the radish male sterile almost all that uses be Ogura cytoplasm types, with male sterile material use it is more next
More universal, the cytoplasm type of radish and other crop in cruciferae is by serious unification.
In crop in cruciferae, nuclear male sterility phenomenon will be lacked relative to cytoplasmic male sterility, only exist at present
Dominant or recessive nucleus male sterility material is found that in the natural population of the crops such as Chinese cabbage, wild cabbage, rape, pakchoi.But
In the research of radish, in natural population by karyogene control the rare report of male sterile material, in the prior art,
In the white AB groups in the dual-purpose through-stone village of radish nuclear male sterility the ratio of sterile plant and fertile plant respectively account for 50% (《China radish is male
Property infertility study summarize》Li Dechao《The Changjiang river vegetables》6th phase in 2006), it is maternal when utilizing it as maternal progress hybrid seeding
Application rate, sown area, transplanting area and plantation density will the 100% male sterile line production of hybrid seeds of Billy when double with
On, and coming into bloom, 50% has powder strain in the maternal row that choose in time, seed production purity could be kept, in female parent of choosing
When having powder strain in row, on the one hand since remaining sterile plant will appear the phenomenon that density is uneven in field, field plant is caused to fall
Volt, farmland running to weeds reduce hybrid seed yield and influence seed quality, on the other hand since powder strain has been gone not in time in field, can cause pollen
Pollution reduces the purity of cenospecies.
Therefore, a kind of radish nuclear male sterility is established using the white AB groups in the dual-purpose through-stone village of radish nuclear male sterility
The selection of the crossbreeding of maintainer makes the cell nucleo-cytoplasmic interreaction male sterility maintainer line 100% that selection and breeding obtain keep not
It educates, and the florescence does not need to manually pull out in female parent and has the problem of powder strain is those skilled in the art's urgent need to resolve during the production of hybrid seeds.
Invention content
In view of this, the present invention provides a kind of selection of radish cell nucleo-cytoplasmic interreaction male sterility maintainer line, the party
Method selection and breeding after mostly generation hybridization, test cross and backcrossing form, and obtained radish nuclear male sterility maintainer energy 100% is kept not
The characteristics of educating, and heredity can be stablized.
The northern kind of dog head tank radish system, becomes dog head through Wuhan Vegetable Research Institute by the selection and breeding of male sterility program
Tank cytoplasmic male sterile line dog A and dog B, the variety characteristic are：Floral leaf, plant type are compacter, leaf green, fleshy root silo shape,
It is up-small and down-big, blue or green head, root long 9-10cm, the thick thickness of root be 5-6cm, Mid-late ripening, 60-70 days breeding time.Stone village ternip system comes
A local varieties derived from Shijiazhuang Region, Hebei Province press radish nuclear male sterility through Wuhan Vegetable Research Institute
Program selection and breeding form a white AB in the genie male sterile line stone village, wherein sterile population be stone A, fertile group be stone B, the product
Kind plate leaf, green, the white skin plain boiled pork of meat, oval tubular, root long 18-22cm, the not thick 4-6cm of root, the chaff heart, Mid-late ripening, breeding time
60 days, variable rate technology was disease-resistant.
To achieve these goals, the present invention adopts the following technical scheme that：
A kind of selection of radish cell nucleo-cytoplasmic interreaction male sterility maintainer line, utilizes Ogura cytoplasmic male sterility system dog head
The maintainer dog head tank B of tank, the white B in the fertile plant stone village in the white dual purpose lines in the emasculation Hou Yushi village are hybridized, and obtain first-filial generation
F1Group；Using the white A in the sterile plant stone village in the white dual purpose lines in the stone village to be maternal, the first-filial generation F1Group carries out test cross for male parent,
First-filial generation F as male parent simultaneously1Group is selfed；Test cross offspring's fertility performance's situation is observed, screens offspring's infertility test cross group
It closes, the male parent self progeny of offspring's infertility test cross combination is continued to be returned with the white B in the stone village, by multiple test cross, return
A new radish cell nucleo-cytoplasmic interreaction male sterility maintainer line stone dog B is obtained after friendship；Its specific steps includes：
1) using as maternal, the Yi Shi village is dual-purpose in vain after the maintainer dog head tank B emasculations of Ogura cytoplasmic male sterility system dog head tank
The white B in the fertile plant stone village in system is hybridized for male parent, obtains first-filial generation F1Group；
2) from the first-filial generation F1Group stone dog-n is selected in group as male parent, using in the radish dual purpose lines
Sterile plant A makees maternal progress test cross, obtains test cross F1Group, and plant the test cross F1Group；
3) the test cross F is observed1Offspring's Fertility segregation situation in group；Select test cross combination FSurvey 1- n, the test cross group
Close FSurvey 1The male parent selfing of-n obtains stone dog-n-n, the stone dog-n-n and combines F with the test crossSurvey 1Sterile plant hybridization in-n, shape
The sterile plant stone dog A-n-n of Cheng Xin；Stone dog-the n-n and white the B in the stone village is returned simultaneously, forms first backcross generation BC1F1Group,
Plant the first backcross generation BC1F1Group；
4) from the first backcross generation BC1F1Shi Gou A-n-n-n groups are selected in group, and as male parent, the stone dog A-n-n makees
Test cross is carried out for female parent, obtains test cross F2Group；
5) observation test cross F2Offspring's Fertility segregation situation in group；Select test cross combination FSurvey 2- n, the test cross combination
FSurvey 2The male parent selfing of-n obtains stone dog-n-n-n-n, the stone dog-n-n-n-n and is used as male parent, and the test cross combines FSurvey 2In-n
Sterile plant F2- 1 is hybridized as female parent；Stone dog-the n-n-n-n and white the B in the stone village is returned simultaneously, forms second backcross generation
BC2F1Group；
6) step 4) and 5) is repeated, until being bred as the radish cell nucleo-cytoplasmic interreaction that sterile plant rate can be kept to reach 100% male
Infertility keeps through-stone dog B.
The present invention is finally obtained one using test cross, backcrossing and field Phenotypic Selection had both had dog head tank B can 100% guarantor
The characteristics of holding infertility, but with almost with the radish cell nucleo-cytoplasmic interreaction male sterility maintainer line stone of the white similar features in the stone village
The dog B and new sterile line stone dog A mating with stone dog B.
Further, in step 2), group's stone dog-n characters are biased to the white dual purpose lines in the stone village.
Further, in step 3), the test cross combines FSurvey 1- n characters are partial to the white dual purpose lines in the stone village, sterile rate
Height, and trait segregation is small after corresponding male parent selfing.
Further, the A-n-n-n of stone dog described in step 4) population characteristics are biased to the white dual purpose lines in the stone village.
Further, the combination of test cross described in step 5) FSurvey 2- n characters are partial to the white dual purpose lines in the stone village, sterile rate
Height, and trait segregation is small after corresponding male parent selfing.
The present disclosure provides a kind of selection of radish cell nucleo-cytoplasmic interreaction male sterility maintainer line, at least with
Lower advantage：
(1) stone dog A infertility rates can be kept up to 100%, and heredity can be stablized；
(2) when carrying out hybrid seeding using sterile through-stone dog A, production cost, the sowing face of sterile through-stone dog A are reduced
Product and plantation density AB dual purpose lines whiter than the Yuan Shi village reduce half；
(3) florescence does not need to manually pull out in female parent and has powder strain during the production of hybrid seeds, and purity of hybrid can reach more than 98%.
(4) breeding objective of the invention is clear and definite, and the procedure of breeding is simple, and breeding research and utilization are convenient.
Description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The embodiment of invention, for those of ordinary skill in the art, without creative efforts, can also basis
The attached drawing of offer obtains other attached drawings.
Fig. 1 attached drawings are the stream of the selection of the radish cell nucleo-cytoplasmic interreaction male sterility maintainer line in the embodiment of the present invention 1
Cheng Tu.
Specific embodiment
The technical solution in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel all other embodiments obtained without making creative work belong to the model that the present invention protects
It encloses.
Embodiment 1
A kind of selection of white skin radish nuclear male sterility maintainer, Breeding Process are as follows：
First Year spring selects the white fertile plant in the stone village from the white white B in the A He Shi village in the white resource garden Zhong Jishi village, the dual-purpose through-stone village
That is stone B makees male parent with stone B；From sterile line dog head tank resource garden, several plants of dogs head tank maintainers of selection, dog B-1, dog B-2,
Dog B-1, dog B-2, dog B-3 ... dogs B-n are carried out emasculation, make female parent with dog B, F is obtained after hybridization by dog B-3 ... dog B-n1
Group, autumn current year field planting.
The next spring, from F1The single plant that several shapes are partial to " Shi Zhuanbai " radish varieties, respectively stone are chosen in group
Dog -1, stone dog -2, stone dog -3 ... ... stone dog-n are used as male parent, while the infertility in selecting the dual-purpose through-stone village white from resource garden
Strain is that stone A is used as female parent, carries out a large amount of test crosses.Test cross generation seed is obtained, in addition its stone dog -1, stone dog -2, stone dog -3 ... ...
Stone dog-n selfings are reserved seed for planting, the combination of autumn current year plantation test cross and its corresponding male parent.The purpose of test cross is to obtain to contain in cytoplasm
Have fertile gene and the single plant containing sterile gene in nucleus.
Spring in third year, observation test cross combination offspring's Fertility segregation situation combine F to there is the test cross of infertilitySurvey 1- n,
Male parent self progeny is labeled as stone dog-n-1, stone dog-n-2, stone dog-n-3 ... stone dog-n-n, stone dog-n-1, stone dog-n-2, stone
On the one hand dog-n-3 ... stone dogs-n-n continues corresponding sterile plant F1- 1 hybridization, is on the one hand returned with stone B, is respectively formed
New sterile plant offspring：Stone dog A-n-1, stone dog A-n-2, stone dog A-n-3 ... stone dog A-n-n and first backcross generation BC1F1Group,
After the plantation of autumn current year, therefrom selection traits are partial to several Plant colonizations of the white B radish varieties of the white A in the stone village or the stone village.
4th year spring, the first backcross generation BC after chosen1F1It chooses several single plants and makees male parent, labeled as stone dog-n-n-
1, stone dog-n-n-2, stone dog-n-n-3 ... stone dog-n-n-n make maternal progress test cross, while stone dog-n- using stone dog A-n-n
N-1, stone dog-n-n-2, stone dog-n-n-3 ... stone dogs-n-n-n selfings, obtain test cross progeny seed and male parent selfed seed, when
The plantation of autumn in year.
5th year spring observed test cross offspring's Fertility segregation situation, and test cross offspring is selected the combination F of infertility occurSurvey 2- n,
Its male parent self progeny, labeled as stone dog-n-n-n-1, stone dog-n-n-n-2, stone dog-n-n-n-3 ... A-n-n-n-n, a side
Face is continued and infertility female parent i.e. stone dog A-n-n-1, stone dog A-n-n-2, stone dog A-n-n-n test crosses, while on the other hand, a stone
Dog-n-n-n-1, stone dog-n-n-n-2, stone dog-n-n-n-3 ... stone dogs-n-n-n-n continue to be returned with stone B, form backcrossing two
For BC2F1Group, autumn current year plant, therefrom the selection traits several Plant colonizations similar to " the white AB in the stone village ".
6th year spring, from the second backcross generation group BC after character determination2F1It chooses several single plants and makees male parent, mark
For stone dog-n-n-n-n-1, stone dog-n-n-n-n-2, stone dog-n-n-n-n-3 ... stone dog-n-n-n-n-n, sterile female parent is utilized
That is stone dog A-n-n-n-1, stone dog A-n-n-n-2, stone dog A-n-n-n-n make female parent and carry out test cross to it；Stone dog-n-n-n- simultaneously
N-1, stone dog-n-n-n-n-2, stone dog-n-n-n-n-3 ... stone dogs-n-n-n-n-n selfings, obtain test cross progeny seed and father
This selfed seed, the plantation of autumn current year.
7th year spring observed test cross offspring's Fertility segregation situation, and test cross offspring is selected the high combination of sterile rate occur,
Male parent self progeny be labeled as stone dog-n-n-n-n-n-1, stone dog-n-n-n-n-n-2, stone dog-n-n-n-n-n-3 ... stones dog-
N-n-n-n-n-n, it is stone dog A-n-n-n-n-1, stone dog A-n-n-n-n-2, stone dog A-n-n- on the one hand to continue maternal with infertility
N-n-n hybridizes, on the other hand, stone dog-n-n-n-n-n-1, stone dog-n-n-n-n-n-2, stone dog-n-n-n-n-n-3 ... stones
Dog-n-n-n-n-n-n continues to be returned with stone B, forms third backcross generation BC3F1Group, autumn current year plantation, therefrom selection traits with
" the white AB in the stone village " similar several Plant colonizations.
8th year spring continued to repeat above work, until " new sterile line stone dog A " group's sterile plant rate reaches 100%,
Its corresponding Agronomic trait for keeping through-stone dog B and " the white AB in the stone village " are almost consistent.
Embodiment 2：
A kind of selection of white skin radish nuclear male sterility maintainer, Breeding Process are as follows：
The fertile plant stone B of the white dual purpose lines in the plantation dog head tank maintainer dog B He Shi village respectively, treats that radish fleshy root is fully ripe
Afterwards, by variety characteristic, to being isolated in solarium, the florescence chooses several plants of dog B-n from dog B groups for field planting after stringent selection,
It will hybridize after dog B-n emasculations with stone B, dog B-n makees female parent, and stone B makees male parent, prepares 10 pairs of combination altogether, is obtained after hybridization and hybridizes one
For F1All combination plantation observations are planted the sterile plant stone A in the white AB dual purpose lines in the stone village by group, autumn.
From prepared 10 couples combination F1In group and sterile plant stone A, several Plant colonizations are selected respectively to isolation solarium,
Florescence using stone A sterile plants as female parent, from hybridization F1Several plants of stone dog-n is selected to carry out test cross as male parent in group, prepares survey altogether
20 pairs of combination is handed over, is individually harvested after seed maturity, number is test cross F1-1、F1-2、F1-3、......F1-20.While stone dog-n is certainly
Friendship is reserved seed for planting.
Second step：Field planting observation test cross F1-1—F1- 20 combinations and its corresponding male parent self progeny, plant simultaneously
The white AB in the stone village, after fleshy root fully expands, therefrom selection traits are biased to the white single plant in the stone village, field planting to isolation solarium, flower respectively
Phase identifies Fertility segregation situation, and 2 pairs of combination for selecting sterile rate high therefrom selects sterile plant to be carried out with corresponding male parent self progeny
Hybridization, number stone dog A-2-1 and stone dog B-2-1, stone dog A-5-1 and stone dog B-5-1 respectively, male parent self progeny at the same respectively with
Fertile plant stone B in the white AB in the stone village be returned primary, singly receipts preservation after seed maturity.
Third walks：Field planting observes filial generation and male parent backcross progeny, select sterile rate high from filial generation and
Character is biased to combination stone dog A-5-1 × stone dog B-5-1 of the stone white AB in the village, and selection traits are close to the white B's in the stone village from backcross progeny
Single plant continues test cross, prepares test cross and combines 10 pairs, while male parent is selfed, the fertility performance of profit observation test cross offspring, selection
High combination stone dog A-5-1-3 × stone dog B-5-1-3, stone dog A-5-1-7 × the stone dog B-5-1-7 of sterile rate, it is corresponding using it
Male parent self progeny hybridization reserves seed for planting, preserves, while corresponding male parent continuation is returned with the white B in the stone village.
4th step：Field planting observation filial generation, backcross progeny and male parent self progeny, continue to repeat test cross,
Backcrossing, being finally obtained one can keep stone A infertility rates to reach 100% and stablize that heredity, character differs not in vain with the stone village completely
Big holding through-stone dog B-5-1-3-4 and its mating new sterile line stone dog A-5-1-3-4, abbreviation stone dog A and stone dog B.
In conclusion so carrying out test cross, backcrossing and field Phenotypic Selection, the nucleus of the white B in the Jiang Shi village is replaced into infertility
Gene, while the feature for keeping the Yuan Shi village white, finally selected a kind of new nucleo-cytoplasmic interreaction male sterility through-stone dog A and
It is corresponding to keep through-stone dog B, their genes both containing dog head tank maintainer, while has the characteristic of the white sterile line in the stone village,
It is measured by combining ability, the combination performance that the combination dual purpose lines white with the Yuan Shi village of preparation are prepared is suitable, after Plant experimental demonstration, greatly
Area promotes and applies.
Each embodiment is described by the way of progressive in this specification, the highlights of each of the examples are with other
The difference of embodiment, just to refer each other for identical similar portion between each embodiment.For method disclosed in embodiment
For, since it is corresponded to the methods disclosed in the examples, so description is fairly simple, related part is said referring to method part
It is bright.
The foregoing description of the disclosed embodiments enables professional and technical personnel in the field to realize or use the present invention.
A variety of modifications of these embodiments will be apparent for those skilled in the art, it is as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, it is of the invention
The embodiments shown herein is not intended to be limited to, and is to fit to and the principles and novel features disclosed herein phase one
The most wide range caused.
Claims (5)
1. a kind of selection of radish cell nucleo-cytoplasmic interreaction male sterility maintainer line, which is characterized in that utilize radish cytoplasmic male
The maintainer dog head tank B of sterile line dog head tank, the white B in the fertile plant stone village in the white dual purpose lines in the emasculation Hou Yushi village are hybridized, are obtained
Obtain first-filial generation F1Group；Using the white A in the sterile plant stone village in the white dual purpose lines in the stone village to be maternal, the first-filial generation F1Group is father
This progress test cross, while the first-filial generation F as male parent1Group is selfed；Test cross offspring's fertility performance's situation is observed, screens offspring
Sterile test cross combination, the male parent self progeny of offspring's infertility test cross combination is continued to be returned with the white B in the stone village, through excessive
A new holding through-stone dog B is obtained after secondary test cross, backcrossing, specific steps include：
1) using after the maintainer dog head tank B emasculations of Ogura cytoplasmic male sterility system dog head tank as maternal, in the white dual purpose lines in the Yi Shi village
The white B in the fertile plant stone village hybridized for male parent, obtain first-filial generation F1Group；
2) from the first-filial generation F1Group stone dog-n is selected to utilize the sterile plant in the radish dual purpose lines as male parent in group
A makees maternal progress test cross, obtains test cross F1Group, and plant the test cross F1Group；
3) the test cross F is observed1Offspring's Fertility segregation situation in group；Select test cross combination FSurvey 1- n, the test cross combination
FSurvey 1The male parent selfing of-n obtains stone dog-n-n, the stone dog-n-n and combines F with the test crossSurvey 1Sterile plant hybridization in-n, forms
New sterile plant stone dog A-n-n；Stone dog-the n-n and white the B in the stone village is returned simultaneously, forms first backcross generation BC1F1Group, kind
Plant the first backcross generation BC1F1 groups；
4) from the first backcross generation BC1F1Shi Gou A-n-n-n groups are selected in group, and as male parent, the stone dog A-n-n is as female
This progress test cross obtains test cross F2Group；
5) observation test cross F2Offspring's Fertility segregation situation in group；Select test cross combination FSurvey 2- n, the test cross combine FSurvey 2- n's
Male parent selfing obtains stone dog-n-n-n-n, the stone dog-n-n-n-n and is used as male parent, and the test cross combines FSurvey 2Sterile plant in-n
F2- 1 is hybridized as female parent；Stone dog-the n-n-n-n and white the B in the stone village is returned simultaneously, forms second backcross generation BC2F1Group
Body；
6) step 4) and 5) is repeated, until being bred as that the radish cell nucleo-cytoplasmic interreaction male sterility that sterile plant rate reaches 100% can be kept
Keep through-stone dog B.
2. a kind of selection of radish cell nucleo-cytoplasmic interreaction male sterility maintainer line according to claim 1, feature exist
In in step 2), group's stone dog-n characters are biased to the white dual purpose lines in the stone village.
3. a kind of selection of radish cell nucleo-cytoplasmic interreaction male sterility maintainer line according to claim 1, feature exist
In in step 3), the test cross combines FSurvey 1- n characters are partial to the white dual purpose lines in the stone village, and sterile rate is high, and corresponding male parent
Trait segregation is small after selfing.
4. a kind of selection of radish cell nucleo-cytoplasmic interreaction male sterility maintainer line according to claim 1, feature exist
In the A-n-n-n of stone dog described in step 4) population characteristics are biased to the white dual purpose lines in the stone village.
5. a kind of selection of radish cell nucleo-cytoplasmic interreaction male sterility maintainer line according to claim 1, feature exist
In the combination of test cross described in step 5) FIt surveys2-n characters are partial to the white dual purpose lines in the stone village, sterile rate height, and corresponding male parent
Trait segregation is small after selfing.
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| CN112535099A (en) * | 2020-12-04 | 2021-03-23 | 四川省农业科学院水稻高粱研究所 | Breeding and utilizing method of black radish germplasm resources |
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