CN105018555A - Preparation method of giant salamander skin collagen peptide - Google Patents
Preparation method of giant salamander skin collagen peptide Download PDFInfo
- Publication number
- CN105018555A CN105018555A CN201510476964.8A CN201510476964A CN105018555A CN 105018555 A CN105018555 A CN 105018555A CN 201510476964 A CN201510476964 A CN 201510476964A CN 105018555 A CN105018555 A CN 105018555A
- Authority
- CN
- China
- Prior art keywords
- giant salamander
- collagen peptide
- skin
- molecular weight
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a preparation method of giant salamander skin collagen peptide. The method comprises the steps that degreasing and deodorization are conducted simultaneously on a giant salamander skin, black skin and impure protein are removed through alkali liquor, under the condition that the temperature ranges from 20 DEG C to 25 DEG C and the pressure ranges from 150 MPa to 300 MPa, pressure maintaining is conducted for 10-60 min, pH of the giant salamander skin processed through ultrahigh pressure is regulated to 8-10, alkaline protease with the weight of 2%-3% of the skin is added, at the temperature of 45-50 DEG C, continuous stirring and enzymolysis are conducted for 3-6 hours, the mixture is boiled for 1-2 min and cooled to 40 DEG C, pH of the mixture is regulated to 4-5, activated carbon with the mass of 1% of the mixture is added, and stirring and decoloration are conducted; under the condition that the rotating speed is 3000-4500 r/min, centrifugation is conducted on the processed mixture for 10-15 min, microfiltration through a filter membrane with the thickness of 0.45 micron, ultrafiltration through an ultrafiltration membrane with the molecular weight cut-off of 4-5 kD and nanofiltration through a nanofiltration membrane with the molecular weight cut-off of 2-3 kD are conducted on the mixture in sequence to obtain filter liquor, the filter liquor is concentrated to be one half of an original sample, and the concentrated liquor is freeze-dried and smashed to obtain giant salamander skin collagen powder. According to the preparation method of the giant salamander skin collagen peptide, the molecular weight of the collagen peptide is small, and over 90% of the molecular weight is smaller than 1000 Dalton.
Description
Technical field
The invention belongs to giant salamander field of deep, be specifically related to a kind of preparation method of giant salamander collagen peptide.
Background technology
China be in the world breeding giant salamander and application giant salamander product country the earliest, giant salamander is classified as the history that Chinese medicinal materials has nearly 2000.Because giant salamander has very high economic worth, the quick vigorous growth of China's giant salamander industry is got up, giant salamander industry also once once maintained an edge in the international market, through the development of nearly decades, begin to take shape industrial system, in the breeding, diseases prevention and treatment etc. of giant salamander, have world lead level especially.But since entering 21 century, along with China joined WTO, the competitive power of China's giant salamander industry is but declining year by year, and domestic giant salamander industry also also exists problems.The road of what China's giant salamander industry was gone on along is a small-scale, extensive style, poor benefit, has not given play to its overall efficiency and industrial advantage.Specifically be mainly manifested in: product comprehensive exploitation and utilize level low, rough-wrought product is many, and high-tech, the high value-added product of deep processing are little, and integral benefit level is difficult to improve.Containing more than 70 kind of natural radioactivity biology in giant salamander body, in edible, medicine, makeup, heath food etc., there are unique research and development to be worth, can also promote that Human Physiology is active, improve human body cell metaboilic level, promote human body protein synthesis, improve immune function of human body, strengthen human disease resistance.For giant salamander field of deep, how carry out the natural nutrition element of giant salamander to extract that to make it to apply more easily be the difficult problem needing solution badly.
Collagen protein is the main component in animal connective tissue, is also the functional protein that Mammals in-vivo content is maximum, distribution is the widest, accounts for 25% ~ 30% of total protein.With the transmission of the formation organized, maturation, cell-tocell, and joint lubrication, wound healing, calcification, blood coagulation and aging etc. have close relationship, in all extensive application such as medical material, makeup, foodstuffs industry.It is generally acknowledged that small-molecular-weight collagen protein has better assimilation effect, therefore conventional collagen peptide in makeup, especially molecular weight lower than 1500 micromolecular collagen peptide.Collagen peptide of the prior art is many to be extracted from the livestock skins such as pig ox and bone, this year extracts collagen peptide and is also subject to the people's attention from fish-skin, fish-bone, but the method extracting collagen peptide disclosed in prior art is extract from native organism, by chemical process and recombinant DNA technology synthesis, extracorporeal hydrolysis protein etc.Due to the difference of different proteolytic enzyme optimum temperuture, optimal pH and iso-electric point, all methods all do not have versatility, especially for this kind of protein with multiple iso-electric point of collagen protein, select suitable method to extract collagen peptide and have difficulty.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of preparation method of giant salamander collagen peptide.
For achieving the above object, the invention provides following technical scheme: a kind of preparation method of giant salamander collagen peptide, step is as follows:
1) get giant salamander skin remove residual fat and wash;
2) the aqueous solution immersion 30 ~ 60min be placed in by giant salamander skin containing 14 ~ 16wt% Trisodium Citrate and 14 ~ 16wt% calcium chloride carries out de-raw meat, and described giant salamander skin and aqueous solution mass ratio are 1:3 ~ 8;
3) alkali lye giant salamander skin that de-raw meat completes being placed in 1.4 ~ 1.6wt% soaks 1 ~ 2h, and post rinse removes black film;
4) by step 3) the giant salamander skin that processed blends, be incorporated as the pure water of giant salamander tare weight amount 5 times again, vacuum sealed package, temperature 20 ~ 25 DEG C, pressurize 10 ~ 60min under pressure 150-300MPa condition, regulate giant salamander skin pH to 8 ~ 10 that uhp treatment is crossed, add the Sumizyme MP of cladding weight 2% ~ 3%, under temperature 45 ~ 50 DEG C of conditions, Keep agitation enzymolysis 3 ~ 6 hours, then batch mixing is boiled 1 ~ 2min, be cooled to 40 DEG C, regulate batch mixing pH to 4 ~ 5 again, add the gac of batch mixing quality 1%, stir decolouring;
5) by step 4) batch mixing that processed is under rotating speed 3000-4500r/min condition, centrifugal 10 ~ 15min, through the filter membrane micro-filtration of 0.45 μm, nanofiltration membrane nanofiltration again through molecular weight cut-off to be the ultra-filtration membrane ultrafiltration of 4 ~ 5kD or molecular weight cut-off be 4 ~ 5kD obtains filtrate, again filtrate is concentrated into 1/2nd of former state, concentrated solution freeze-drying is also pulverized and obtained giant salamander collagen peptide.
Preferably, step 2) the described aqueous solution is containing 15wt% Trisodium Citrate and 15wt% calcium chloride.
Preferably, step 3) described alkali lye is one or more in sodium hydroxide, sodium carbonate, water glass, phosphoric acid salt, Sodium Tetraborate.
Preferably, described step 4) carry out as follows: by step 3) the giant salamander skin that processed blends, be incorporated as the pure water of giant salamander tare weight amount 5 times again, vacuum sealed package, temperature 20 ~ 25 DEG C, pressurize 60min under pressure 200MPa condition, the giant salamander skin pH to 9 regulating uhp treatment to cross, adds the Sumizyme MP of cladding weight 3%, under temperature 50 C condition, Keep agitation enzymolysis 4 hours, again batch mixing is boiled 1min, be cooled to 40 DEG C, then regulate batch mixing pH to 4 ~ 5, add the gac of batch mixing quality 1%, stir decolouring.
Preferably, described step 5) described in ultra-filtration membrane molecular weight cut-off be 5kD.
Preferably, described step 5) described in nanofiltration membrane molecular weight cut-off be 2kD.
Beneficial effect of the present invention is: 1, by Trisodium Citrate and calcium chloride defishying, and removing peculiar smell, improves product quality; 2, remove atrament in giant salamander skin by dipping by lye, be conducive to alleviating follow-up decoloration process difficulty, ensure that the good colour of collagen peptide, eliminate a large amount of grease simultaneously; 3, uhp treatment makes collagen protein appropriateness sex change in giant salamander skin, is conducive to the effect of proteolytic enzyme, improves enzymolysis product yield and (calculate with new fresh hide, collagen peptide productive rate reaches more than 15%), improve the biological activity of collagen peptide, raise the efficiency, reduce energy consumption; 4, collagen protein peptide molecular weight little (more than 90% molecular weight is less than 1000 dalton after testing) is prepared in this invention, improves absorption of human body utilization ratio; 5, this invention collagen peptide preparation technology, safety, environmental protection.
Embodiment
Below the preferred embodiments of the present invention are described in detail.
Embodiment 1
Giant salamander collagen peptide is prepared as follows:
1) get 1kg giant salamander skin remove residual fat and wash;
2) aqueous solution be placed in by giant salamander skin containing 15wt% Trisodium Citrate and 15wt% calcium chloride soaks 60min and carries out de-raw meat, and described giant salamander skin and aqueous solution mass ratio are 1:3;
3) sodium hydroxide solution giant salamander skin that de-raw meat completes being placed in 1.4wt% soaks 2h, and post rinse removes black film;
4) by step 3) the giant salamander skin that processed blends, then is incorporated as the pure water of giant salamander tare weight amount 5 times, vacuum sealed package, temperature 20 ~ 25 DEG C, pressurize 60min under pressure 150MPa condition, regulates the giant salamander skin pH to 9 that uhp treatment is crossed, add the Sumizyme MP of cladding weight 2%, under temperature 50 C condition, Keep agitation enzyme 6 hours, then batch mixing is boiled 1min, be cooled to 40 DEG C, regulate batch mixing pH to be 5 again, add the gac of batch mixing quality 1%, stir decolouring;
5) by step 4) batch mixing that processed is under rotating speed 3000r/min condition, centrifugal 15min, again successively through filter membrane micro-filtration, the molecular weight cut-off amount of 0.45 μm be the ultra-filtration membrane ultrafiltration of 5kD, molecular weight cut-off amount is that the nanofiltration membrane nanofiltration of 3kD obtains filtrate, again filtrate is concentrated into 1/2nd of former state, concentrated solution freeze-drying is also pulverized and obtained giant salamander collagen peptide.
Embodiment 2
Giant salamander collagen peptide is prepared as follows:
1) get 1kg giant salamander skin remove residual fat and wash;
2) aqueous solution be placed in by giant salamander skin containing 16wt% Trisodium Citrate and 14wt% calcium chloride soaks 30min and carries out de-raw meat, and described giant salamander skin and aqueous solution mass ratio are 1:5;
3) the sodium carbonate liquid giant salamander skin that de-raw meat completes being placed in 1.5wt% soaks 1h, and post rinse removes black film;
4) by step 3) the giant salamander skin that processed blends, then is incorporated as the pure water of giant salamander tare weight amount 5 times, vacuum sealed package, temperature 20 ~ 25 DEG C, pressurize 10min under pressure 300MPa condition, regulates the giant salamander skin pH to 10 that uhp treatment is crossed, add the Sumizyme MP of cladding weight 3%, under temperature 45 C condition, Keep agitation enzymolysis 5 hours, then batch mixing is boiled 1min, be cooled to 40 DEG C, regulate batch mixing pH value to be 5 again, add the gac of batch mixing quality 1%, stir decolouring;
5) by step 4) batch mixing that processed is under rotating speed 4500r/min condition, centrifugal 10min, again successively through filter membrane micro-filtration, the molecular weight cut-off amount of 0.45 μm be the ultra-filtration membrane ultrafiltration of 4kD, molecular weight cut-off amount is that the nanofiltration membrane nanofiltration of 2kD obtains filtrate, again filtrate is concentrated into 1/2nd of former state, concentrated solution freeze-drying is also pulverized and obtained giant salamander collagen peptide.
Embodiment 3
Giant salamander collagen peptide is prepared as follows:
1) get 1kg giant salamander skin remove residual fat and wash;
2) aqueous solution be placed in by giant salamander skin containing 14wt% Trisodium Citrate and 16wt% calcium chloride soaks 40min and carries out de-raw meat, and described giant salamander skin and aqueous solution mass ratio are 1:8;
3) alkali lye giant salamander skin that de-raw meat completes being placed in 1.6wt% soaks 2h, and post rinse removes black film;
4) by step 3) the giant salamander skin that processed blends, then is incorporated as the pure water of giant salamander tare weight amount 5 times, vacuum sealed package, temperature 20 ~ 25 DEG C, pressurize 40min under pressure 200MPa condition, regulates the giant salamander skin pH to 8 that uhp treatment is crossed, add the Sumizyme MP of cladding weight 2.5%, under temperature 40 DEG C of conditions, Keep agitation enzymolysis 6 hours, then batch mixing is boiled 1.5min, be cooled to 40 DEG C, regulate batch mixing pH to 5 again, add the gac of batch mixing quality 1%, stir decolouring;
5) by step 4) batch mixing that processed is under rotating speed 4500r/min condition, centrifugal 15min, again successively through filter membrane micro-filtration, the molecular weight cut-off amount of 0.45 μm be the ultra-filtration membrane ultrafiltration of 5kD, molecular weight cut-off amount is that the nanofiltration membrane nanofiltration of 3kD obtains filtrate, again filtrate is concentrated into 1/2nd of former state, concentrated solution freeze-drying is also pulverized and obtained giant salamander collagen peptide.
Comparative example 1
Compared with embodiment 1, difference be carry out step 3) base extraction, other steps are consistent.
Comparative example 2
Compared with embodiment 1, difference be carry out step 4) uhp treatment, other steps are consistent.
Collagen peptide analyzing and testing and color and luster judgement are carried out to above embodiment and comparative example:
1, the mensuration of polypeptide molecular weight
Detection method: by gel chromatography determining molecular weight, after selecting the Tris-HCl elutriant Balance Treatment SPhedexG-25 gel column of 0.1mol/L in process of the test, under the wavelength of 220nm, detectable level is the reference material of 2mg/ml: reductive glutathione (307Da), oxytocin (1007Da), hyperglycemic-glycogenolytic factor (3485Da) and Regular Insulin (5808Da), according to reference material molecular weight logarithm and elution volume, matching obtains regression equation and is: y=-40.238x+194.57 (R2=0.9909); Wherein, y is elution volume, and x is the logarithmic value of corresponding molecular weight.It is 5mg/ml that each embodiment and comparative example gained polypeptide are formulated as concentration respectively, distinguish sample introduction wash-out under these conditions, peak volume will be gone out and bring typical curve into, molecular weight analyte and distribution range thereof, by peak area normalization method, determine the percentage contents of different molecular weight protein peptide, acquired results is as shown in table 1.
Table 1: polypeptide molecular weight distribution and apparent color and luster
| Embodiment is numbered | Color and luster | Be greater than 3kD | 2～3kD | 1～2kD | Be less than 1kD |
| Embodiment 1 | Milky white ~ yellow fraction | ─ | 2.1％ | 3.5％ | 94.4％ |
| Embodiment 2 | Milky white ~ yellow fraction | ─ | 2.5％ | 4.6％ | 92.9％ |
| Embodiment 3 | Milky white ~ yellow fraction | ─ | 2.9％ | 5.4％ | 91.7％ |
| Comparative example 1 | Yellow fraction is partially black | ─ | 2.2％ | 3.9％ | 93.9％ |
| Comparative example 2 | Milky white ~ yellow fraction | 11.3％ | 19.2％ | 20.6％ | 48.9％ |
As known from Table 1, its molecular weight of giant salamander collagen peptide prepared by the present invention is mainly concentrated on and is less than within the scope of 1kD, and good colour, normally between milky white and yellow fraction.
2, skin permeation test in vitro
Get the Wistar rat of body weight 150g, anesthesia, picks clean fine hair, careful peeling, is separated subcutaneus adipose tissue and mucous tissue, repeatedly rinses several times with physiological saline; Dispersion device and medium select modified Franz diffusion cells; Dispersive medium selects physiological saline, provides the environment similar in human body.By each embodiment and comparative example 2 gained collagen peptide soluble in water, be mixed with the polypeptide solution that concentration is 0.8%, equal-volume uniform application is in experiment material.Transdermal absorption operation conveniently, under detection 215nm wavelength, the ultraviolet light absorption angle value of polypeptide solution, obtains transmitance by formula.Experimentally result is known: the transmitance of embodiment 1 ~ 3 gained collagen peptide is respectively 83.3%, 79.5% and 81.7%; The transmitance of comparative example 2 gained collagen peptide is respectively 50.9%.Good from the percutaneous absorbability of the known gained collagen egg peptide of the present invention of test example 2.
What finally illustrate is, above preferred embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although by above preferred embodiment to invention has been detailed description, but those skilled in the art are to be understood that, various change can be made to it in the form and details, and not depart from claims of the present invention limited range.
Claims (6)
1. a preparation method for giant salamander collagen peptide, is characterized in that, step is as follows:
1) get giant salamander skin remove residual fat and wash;
2) the aqueous solution immersion 30 ~ 60min be placed in by giant salamander skin containing 14 ~ 16wt% Trisodium Citrate and 14 ~ 16wt% calcium chloride carries out de-raw meat, and described giant salamander skin and aqueous solution mass ratio are 1:3 ~ 8;
3) alkali lye giant salamander skin that de-raw meat completes being placed in 1.4 ~ 1.6wt% soaks 1 ~ 2h, and post rinse removes black film;
4) by step 3) the giant salamander skin that processed blends, be incorporated as the pure water of giant salamander tare weight amount 5 times again, vacuum sealed package, temperature 20 ~ 25 DEG C, pressurize 10 ~ 60min under pressure 150-300MPa condition, regulate giant salamander skin pH to 8 ~ 10 that uhp treatment is crossed, add the Sumizyme MP of cladding weight 2% ~ 3%, under temperature 45 ~ 50 DEG C of conditions, Keep agitation enzymolysis 3 ~ 6 hours, then batch mixing is boiled 1 ~ 2min, be cooled to 40 DEG C, regulate batch mixing pH to 4 ~ 5 again, add the gac of batch mixing quality 1%, stir decolouring;
5) by step 4) batch mixing that processed is under rotating speed 3000-4500r/min condition, centrifugal 10 ~ 15min, again successively through filter membrane micro-filtration, the molecular weight cut-off of 0.45 μm be the ultra-filtration membrane ultrafiltration of 4 ~ 5kD, molecular weight cut-off is that the nanofiltration membrane nanofiltration of 2-3kD obtains filtrate, again filtrate is concentrated into 1/2nd of former state, concentrated solution freeze-drying is also pulverized and obtained giant salamander collagen peptide.
2. the preparation method of giant salamander collagen peptide according to claim 1, is characterized in that, step 2) the described aqueous solution is containing 15wt% Trisodium Citrate and 15wt% calcium chloride.
3. the preparation method of giant salamander collagen peptide according to claim 1, is characterized in that, step 3) described alkali lye is one or more in sodium hydroxide, sodium carbonate, water glass, phosphoric acid salt, Sodium Tetraborate.
4. the preparation method of giant salamander collagen peptide according to claim 1, it is characterized in that, described step 4) carry out as follows: by step 3) the giant salamander skin that processed blends, be incorporated as the pure water of giant salamander tare weight amount 5 times again, vacuum sealed package, temperature 20 ~ 25 DEG C, pressurize 60min under pressure 200MPa condition, regulate the giant salamander skin pH to 9 that uhp treatment is crossed, add the Sumizyme MP of cladding weight 3%, under temperature 50 C condition, Keep agitation enzymolysis 4 hours, again batch mixing is boiled 1min, be cooled to 40 DEG C, regulate batch mixing pH to 4 ~ 5 again, add the gac of batch mixing quality 1%, stir decolouring.
5. the preparation method of giant salamander collagen peptide according to claim 1, is characterized in that, described step 5) described in ultra-filtration membrane molecular weight cut-off be 5kD.
6. the preparation method of giant salamander collagen peptide according to claim 1, is characterized in that, described step 5) described in nanofiltration membrane molecular weight cut-off be 2kD.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510476964.8A CN105018555A (en) | 2015-08-06 | 2015-08-06 | Preparation method of giant salamander skin collagen peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510476964.8A CN105018555A (en) | 2015-08-06 | 2015-08-06 | Preparation method of giant salamander skin collagen peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105018555A true CN105018555A (en) | 2015-11-04 |
Family
ID=54408854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510476964.8A Pending CN105018555A (en) | 2015-08-06 | 2015-08-06 | Preparation method of giant salamander skin collagen peptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105018555A (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105233271A (en) * | 2015-11-19 | 2016-01-13 | 洛阳师范学院 | Anti-scaring andrias skin collagen burn cream and preparation method thereof |
| CN105249312A (en) * | 2015-11-08 | 2016-01-20 | 陕西理工学院 | Color removal method of giant salamander processed meat skin |
| CN106106972A (en) * | 2016-06-27 | 2016-11-16 | 张博 | A kind of collagen product and preparation method thereof |
| CN107142617A (en) * | 2017-06-11 | 2017-09-08 | 成都吱吖科技有限公司 | It is a kind of to detect simultaneously tunica fibrosa of adsorptive nicotine and preparation method thereof |
| CN107326056A (en) * | 2017-07-21 | 2017-11-07 | 重庆贡龙大鲵生物科技有限公司 | The preparation method of giant salamander polypeptide powder |
| CN107951036A (en) * | 2017-11-13 | 2018-04-24 | 凯瑞欧（天津）生物技术有限公司 | A kind of polypeptide product |
| CN109007480A (en) * | 2018-09-14 | 2018-12-18 | 合肥工业大学 | A kind of Functional Polypeptides potato slurry beverage and preparation method thereof |
| CN109096392A (en) * | 2018-09-03 | 2018-12-28 | 艾苛密（上海）健康科技股份有限公司 | Skin repair collagen and preparation method thereof |
| CN109627325A (en) * | 2019-01-04 | 2019-04-16 | 广东海洋大学 | A kind of preparation method and applications of gradient collagen and collagen polypeptide molecule |
| CN111172226A (en) * | 2020-03-09 | 2020-05-19 | 中健智诊(重庆)生物研究院 | Collagen peptide for intestinal absorption and extraction method and application thereof |
| CN111184194A (en) * | 2020-03-19 | 2020-05-22 | 深圳市九然生物科技有限公司 | Giant salamander peptide dried konjac and production method thereof |
| CN111248447A (en) * | 2020-01-16 | 2020-06-09 | 辽宁太爱肽生物工程技术有限公司 | Giant salamander peptide-containing composition for improving human immunity and application thereof |
| CN111772195A (en) * | 2020-07-15 | 2020-10-16 | 张兆熙 | Giant salamander collagen peptide, preparation method and preparation thereof |
| CN113115853A (en) * | 2021-04-26 | 2021-07-16 | 张家界民康生物科技有限公司 | Preparation method of artificially-cultured second-generation giant salamander peptide freeze-dried powder and product thereof |
| CN113136330A (en) * | 2021-05-15 | 2021-07-20 | 德州蓝力生物技术有限公司 | Production and treatment device and process of cod skin collagen peptide |
| CN114794297A (en) * | 2022-05-23 | 2022-07-29 | 甘肃农业大学 | Edible animal skin liquid collagen and liquid collagen jelly prepared from edible animal skin liquid collagen |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1100825A (en) * | 1993-09-25 | 1995-03-29 | C&T科技发展有限公司 | Intelligent translating system |
| CN104068204A (en) * | 2014-06-16 | 2014-10-01 | 四川溪源水产养殖有限公司 | Preparation method of giant salamander polypeptides |
-
2015
- 2015-08-06 CN CN201510476964.8A patent/CN105018555A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1100825A (en) * | 1993-09-25 | 1995-03-29 | C&T科技发展有限公司 | Intelligent translating system |
| CN104068204A (en) * | 2014-06-16 | 2014-10-01 | 四川溪源水产养殖有限公司 | Preparation method of giant salamander polypeptides |
Non-Patent Citations (4)
| Title |
|---|
| 叶欣等: "人工饲养大鲵皮胶原蛋白肽提取工艺研究", 《肉类工业》 * |
| 张慜: "《生鲜食品保质干燥新技术理论与实践》", 30 April 2009, 化学工业出版社 * |
| 胡爱军等: "酶法水解真鲷鱼皮和鱼鳞制取胶原蛋白肽", 《食品工业》 * |
| 董新红等: "超高压技术在蛋白质食品加工中的应用", 《食品工业科技》 * |
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105249312B (en) * | 2015-11-08 | 2019-05-14 | 陕西理工学院 | The discoloration method of giant salamander processing pork skin |
| CN105249312A (en) * | 2015-11-08 | 2016-01-20 | 陕西理工学院 | Color removal method of giant salamander processed meat skin |
| CN105233271A (en) * | 2015-11-19 | 2016-01-13 | 洛阳师范学院 | Anti-scaring andrias skin collagen burn cream and preparation method thereof |
| CN105233271B (en) * | 2015-11-19 | 2018-08-31 | 洛阳师范学院 | A kind of anti-scar salamander collagen burn cream and preparation method thereof |
| CN106106972A (en) * | 2016-06-27 | 2016-11-16 | 张博 | A kind of collagen product and preparation method thereof |
| CN107142617A (en) * | 2017-06-11 | 2017-09-08 | 成都吱吖科技有限公司 | It is a kind of to detect simultaneously tunica fibrosa of adsorptive nicotine and preparation method thereof |
| CN107326056A (en) * | 2017-07-21 | 2017-11-07 | 重庆贡龙大鲵生物科技有限公司 | The preparation method of giant salamander polypeptide powder |
| CN107951036A (en) * | 2017-11-13 | 2018-04-24 | 凯瑞欧（天津）生物技术有限公司 | A kind of polypeptide product |
| CN109096392A (en) * | 2018-09-03 | 2018-12-28 | 艾苛密（上海）健康科技股份有限公司 | Skin repair collagen and preparation method thereof |
| CN109007480A (en) * | 2018-09-14 | 2018-12-18 | 合肥工业大学 | A kind of Functional Polypeptides potato slurry beverage and preparation method thereof |
| CN109627325A (en) * | 2019-01-04 | 2019-04-16 | 广东海洋大学 | A kind of preparation method and applications of gradient collagen and collagen polypeptide molecule |
| CN111248447A (en) * | 2020-01-16 | 2020-06-09 | 辽宁太爱肽生物工程技术有限公司 | Giant salamander peptide-containing composition for improving human immunity and application thereof |
| CN111172226A (en) * | 2020-03-09 | 2020-05-19 | 中健智诊(重庆)生物研究院 | Collagen peptide for intestinal absorption and extraction method and application thereof |
| CN111184194A (en) * | 2020-03-19 | 2020-05-22 | 深圳市九然生物科技有限公司 | Giant salamander peptide dried konjac and production method thereof |
| CN111772195A (en) * | 2020-07-15 | 2020-10-16 | 张兆熙 | Giant salamander collagen peptide, preparation method and preparation thereof |
| CN111772195B (en) * | 2020-07-15 | 2022-05-20 | 张兆熙 | Giant salamander collagen peptide, preparation method and preparation thereof |
| CN113115853A (en) * | 2021-04-26 | 2021-07-16 | 张家界民康生物科技有限公司 | Preparation method of artificially-cultured second-generation giant salamander peptide freeze-dried powder and product thereof |
| CN113136330A (en) * | 2021-05-15 | 2021-07-20 | 德州蓝力生物技术有限公司 | Production and treatment device and process of cod skin collagen peptide |
| CN114794297A (en) * | 2022-05-23 | 2022-07-29 | 甘肃农业大学 | Edible animal skin liquid collagen and liquid collagen jelly prepared from edible animal skin liquid collagen |
| CN114794297B (en) * | 2022-05-23 | 2023-06-06 | 甘肃农业大学 | Edible animal skin liquid collagen and liquid collagen jelly prepared from same |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105018555A (en) | Preparation method of giant salamander skin collagen peptide | |
| CN102533914B (en) | High-purity fishy smell and foreign odor-free fish collagen protein peptide and preparation method thereof | |
| CN104004813B (en) | A kind of preparation of mushroom biologically active peptide | |
| CN103843970B (en) | Production method for preparing ossein oligopeptide meal, bone oil and bone meal | |
| CN104513843B (en) | A kind of combined preparation process of polysaccharide and protein peptides | |
| CN105039483A (en) | Method for preparing fishskin fish-scale collagen protein | |
| CN106282287B (en) | Method for extracting bioactive polypeptide of ginkgo | |
| CN107095313B (en) | Collagen oral liquid | |
| CN102994598B (en) | Weak bitter corn oligopeptide with high content of alanine and leucine, and preparation method thereof | |
| CN101363040A (en) | Method for preparing collagen protein | |
| CN104726527A (en) | Preparation technology for producing collagen through enzymolysis of fish scales | |
| CN107164439A (en) | A kind of large-scale preparation method of fish scale NTx protein peptides suitable for cosmetics | |
| CN105969830A (en) | Method for extracting active collagen peptide from pigskin | |
| CN108165583A (en) | Matrimony vine enzymatic hydrolysis and fermentation liquid and preparation process and the application in cosmetics | |
| CN104357522A (en) | Method for extracting collagen by using ecdysis of giant salamander | |
| CN106359839A (en) | Extraction method of oyster peptides | |
| CN107188990A (en) | The method that chondroitin sulfate is extracted in sturgeon bone | |
| CN105420325A (en) | Placenta polypeptide preparation method | |
| CN109486890A (en) | A kind of technique preparing Fish protein hybrid peptide from crude fish protein | |
| CN104745664A (en) | Preparation process of animal placenta extract | |
| CN103099248A (en) | Method for preparing oyster active component | |
| CN106307532A (en) | Rosa roxburghii tratt composite enzyme and preparation method thereof | |
| CN103952457A (en) | Method for extracting active collagen peptide with low molecular weight from chicken skin | |
| CN110951809A (en) | Process for preparing quinoa wheat polypeptide powder | |
| CN105420324B (en) | A kind of preparation method of high molecular weight silver carp collagen peptide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151104 |